WO2003054207A3 - Fermentation process for the preparation of l-amino acids using coryneform bacteria - Google Patents

Fermentation process for the preparation of l-amino acids using coryneform bacteria Download PDF

Info

Publication number
WO2003054207A3
WO2003054207A3 PCT/EP2002/013287 EP0213287W WO03054207A3 WO 2003054207 A3 WO2003054207 A3 WO 2003054207A3 EP 0213287 W EP0213287 W EP 0213287W WO 03054207 A3 WO03054207 A3 WO 03054207A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
bacteria
preparation
amino acids
coryneform bacteria
Prior art date
Application number
PCT/EP2002/013287
Other languages
French (fr)
Other versions
WO2003054207A2 (en
Inventor
Mike Farwick
Brigitte Bathe
Jennifer Brehme
Natalie Schischka
Walter Pfefferle
Original Assignee
Degussa
Mike Farwick
Brigitte Bathe
Jennifer Brehme
Natalie Schischka
Walter Pfefferle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa, Mike Farwick, Brigitte Bathe, Jennifer Brehme, Natalie Schischka, Walter Pfefferle filed Critical Degussa
Priority to AU2002356730A priority Critical patent/AU2002356730A1/en
Priority to EP02805292A priority patent/EP1456392A2/en
Publication of WO2003054207A2 publication Critical patent/WO2003054207A2/en
Publication of WO2003054207A3 publication Critical patent/WO2003054207A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a process for the preparation of L-amino acids in which the following steps are carried out: a) fermentation of coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for a small integral C4-dicarboxylate membrane transport protein and/or the gene coding for the 2-oxoglutarate/malate translocator are attenuated; b) enrichment of the desired L-amino acid in the medium or in the cells of the bacteria; and c) isolation of the L-amino acid, and optionally bacteria are used in which other genes of the biosynthetic pathway of the desired L-amino acid are additionally enhanced, or bacteria are used in which the metabolic pathways that decrease the formation of the desired L-amino acid are at least partially switched off.
PCT/EP2002/013287 2001-12-21 2002-11-26 Fermentation process for the preparation of l-amino acids using coryneform bacteria WO2003054207A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002356730A AU2002356730A1 (en) 2001-12-21 2002-11-26 Fermentation process for the preparation of l-amino acids using coryneform bacteria
EP02805292A EP1456392A2 (en) 2001-12-21 2002-11-26 Fermentation process for the preparation of l-amino acids using coryneform bacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2001163167 DE10163167A1 (en) 2001-12-21 2001-12-21 Process for the fermentative production of L-amino acids and use of coryneform bacteria
DE10163167.7 2001-12-21

Publications (2)

Publication Number Publication Date
WO2003054207A2 WO2003054207A2 (en) 2003-07-03
WO2003054207A3 true WO2003054207A3 (en) 2004-01-29

Family

ID=7710306

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/013287 WO2003054207A2 (en) 2001-12-21 2002-11-26 Fermentation process for the preparation of l-amino acids using coryneform bacteria

Country Status (4)

Country Link
EP (1) EP1456392A2 (en)
AU (1) AU2002356730A1 (en)
DE (1) DE10163167A1 (en)
WO (1) WO2003054207A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2007873B1 (en) 2006-04-18 2015-11-18 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE
US10428359B2 (en) * 2016-10-03 2019-10-01 Ajinomoto Co, Inc. Method for producing L-amino acid

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4420782C1 (en) * 1994-06-15 1995-08-17 Fluegge Ulf Ingo Prof Dr New DNA encoding a 2-oxoglutarate-malate translocator
WO2000031281A2 (en) * 1998-11-21 2000-06-02 Basf Aktiengesellschaft Dna sequence encoding a glutamate/malate translocator, plasmids, bacteria, yeasts and plants containing said transporter
WO2001000805A2 (en) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
WO2001002584A1 (en) * 1999-07-02 2001-01-11 Ajinomoto Co.,Inc. Dna encoding sucrose pts enzyme ii
EP1087015A2 (en) * 1999-09-15 2001-03-28 Degussa-Hüls Aktiengesellschaft Cloning and uses of a novel nucleotide sequence coding for glucose-6-phosphate isomerase (gpi) from bacteria
EP1108790A2 (en) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Novel polynucleotides
WO2002024915A1 (en) * 2000-09-19 2002-03-28 Degussa Ag Dcta (c4-dicarboxylate transporter) from corynebacterium glutamicum

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4420782C1 (en) * 1994-06-15 1995-08-17 Fluegge Ulf Ingo Prof Dr New DNA encoding a 2-oxoglutarate-malate translocator
WO2000031281A2 (en) * 1998-11-21 2000-06-02 Basf Aktiengesellschaft Dna sequence encoding a glutamate/malate translocator, plasmids, bacteria, yeasts and plants containing said transporter
WO2001000805A2 (en) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
WO2001002584A1 (en) * 1999-07-02 2001-01-11 Ajinomoto Co.,Inc. Dna encoding sucrose pts enzyme ii
EP1197555A1 (en) * 1999-07-02 2002-04-17 Ajinomoto Co., Inc. Dna encoding sucrose pts enzyme ii
EP1087015A2 (en) * 1999-09-15 2001-03-28 Degussa-Hüls Aktiengesellschaft Cloning and uses of a novel nucleotide sequence coding for glucose-6-phosphate isomerase (gpi) from bacteria
EP1108790A2 (en) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Novel polynucleotides
WO2002024915A1 (en) * 2000-09-19 2002-03-28 Degussa Ag Dcta (c4-dicarboxylate transporter) from corynebacterium glutamicum

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DAVIES S J ET AL.: "Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli.", JOURNAL OF BACTERIOLOGY, vol. 181, no. 18, September 1999 (1999-09-01), pages 5624 - 5635, XP002245249, ISSN: 0021-9193 *
EGGELING L ET AL.: "Transport mutants and transport genes of Corynebacterium glutamicum", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 782, 15 May 1996 (1996-05-15), pages 191 - 201, XP000675250, ISSN: 0077-8923 *
HUA Q ET AL.: "Metabolic control analysis for lysine synthesis using Corynebacterium glutamicum and experimental verification.", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 90, no. 2, August 2000 (2000-08-01), pages 184 - 192, XP002245250, ISSN: 1389-1723 *
JETTEN M S M ET AL.: "Recent advances in the physiology and genetics of amino acid-producing bacteria", CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, vol. 15, no. 1, 1995, pages 73 - 103, XP000613291, ISSN: 0738-8551 *
KRAMER R: "Genetic and physiological approaches for the production of amino acids", JOURNAL OF BIOTECHNOLOGY, vol. 45, no. 1, 12 February 1996 (1996-02-12), pages 1 - 21, XP004036833, ISSN: 0168-1656 *
POS K M ET AL: "The Escherichia coli citrate carrier CitT: a member of a novel eubacterial transporter family related to the 2-oxoglutarate/malate translocator from spinach chloroplasts.", JOURNAL OF BACTERIOLOGY, vol. 180, no. 16, August 1998 (1998-08-01), pages 4160 - 4165, XP002254180, ISSN: 0021-9193 *
RABUS R ET AL.: "TRAP transporters: An ancient family of extracytoplasmic solute-receptor-dependent secondary active transporters", MICROBIOLOGY, vol. 145, no. 12, December 1999 (1999-12-01), pages 3431 - 3445, XP002245252, ISSN: 1350-0872 *
SAIER M H ET AL.: "Phylogenetic characterization of novel transport protein families revealed by genome analyses", BIOCHIMICA ET BIOPHYSICA ACTA. REVIEWS ON BIOMEMBRANES, vol. 1422, no. 1, 25 February 1999 (1999-02-25), pages 1 - 56, XP004281727, ISSN: 0304-4157 *
SOMERVILLE S C ET AL.: "An Arabidopsis thaliana mutant defective in chloroplast dicarboxylate transport", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 80, March 1983 (1983-03-01), pages 1290 - 1294, XP002137009, ISSN: 0027-8424 *
WYBORN N R ET AL: "Topological analysis of DctQ, the small integral membrane protein of the C4-dicarboxylate TRAP transporter of Rhodobacter capsulatus.", FEMS MICROBIOLOGY LETTERS, vol. 194, no. 1, 1 January 2001 (2001-01-01), pages 13 - 17, XP002245251, ISSN: 0378-1097 *

Also Published As

Publication number Publication date
AU2002356730A1 (en) 2003-07-09
DE10163167A1 (en) 2003-07-03
EP1456392A2 (en) 2004-09-15
WO2003054207A2 (en) 2003-07-03

Similar Documents

Publication Publication Date Title
WO2003008608A3 (en) Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an enhanced phoe gene
WO2002029080A3 (en) Fermentation process for the preparation of l-amino acids using strains of the family enterobacteriaceae
CN100529057C (en) Method for producing L-amino acid by colibacillus bacteria
WO2002081721A3 (en) Process for the production of l-amino acids using strains of the family enterobacteriaceae that contain an attenuated dgsa gene
WO2003008603A3 (en) Process for the preparation of l-amino acids using strains of the enterobacteri acea family which contain an attenuated aspa gene
EP1266966A3 (en) Method for producing L-lysine or L-arginine by using methanol assimilating bacterium
PL1608764T3 (en) A process for the production of l-amino acids using strains of the enterobacteriaceae family which overexpress the galp gene, coding for a galactose proton symporter
WO2002036797A3 (en) Process for the fermentative preparation of l-amino acids using strains of the enterobacteriaceae family
AU4727797A (en) Microbial preparation of substances from aromatic metabolism/i
WO2002086137A3 (en) Process for the production of l-amino acids by fermentation using coryneform bacteria
MX244419B (en)
WO2003023044A3 (en) Process for the production of l-amino acids using coryneform bacteria
WO2003054207A3 (en) Fermentation process for the preparation of l-amino acids using coryneform bacteria
ATE274575T1 (en) PROCESS FOR THE FERMENTATIVE PRODUCTION OF D-PANTOTHENIC ACID USING CORYNEFORM BACTERIA
AU6431600A (en) Process for the fermentative preparation of l-amino acids with amplification of the gnd gene
ATE357532T1 (en) METHOD FOR PRODUCING L-LYSINE USING CORYNEFORMER BACTERIA THAT CONTAIN AN ATTENUATED MALATE ENZYME GENE
EP1788075A4 (en) Industrially useful microorganism
AU2003290053A1 (en) Process for the fermentative preparation of l-amino acids using strains of the enterobacteriaceae family which contain an attenuated yjgf orf sequence
WO2002038788A3 (en) Process for the fermentative preparation of l-amino acids using coryneform bacteria
PL358913A1 (en) Process for the fermentative preparation of l-amino acids with amplification of the tkt gene
WO2003014370A3 (en) Process for the fermentative preparation of l-amino acids using coryneform bacteria
WO2002074966A3 (en) Process for the preparation of l-amino acids by using coryneform bacteria
WO2003076636A3 (en) Process for the preparation of l-amino acids using strains of the enterobacteriaceae family
WO2003076629A3 (en) Process for the preparation of l-amino acids using strains of the enterobacteriaceae family
EP1077261A3 (en) A process for the fermentative preparation of metabolic products and for the nucleotide sequences encoding sod

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002805292

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2002805292

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2002805292

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP