WO2003050501A3 - Indirect fluctuation screening - Google Patents

Indirect fluctuation screening Download PDF

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Publication number
WO2003050501A3
WO2003050501A3 PCT/US2002/025261 US0225261W WO03050501A3 WO 2003050501 A3 WO2003050501 A3 WO 2003050501A3 US 0225261 W US0225261 W US 0225261W WO 03050501 A3 WO03050501 A3 WO 03050501A3
Authority
WO
WIPO (PCT)
Prior art keywords
indirect
outside
cells
products
activity
Prior art date
Application number
PCT/US2002/025261
Other languages
French (fr)
Other versions
WO2003050501A2 (en
Inventor
Andrei V Gudkov
Original Assignee
Univ Illinois
Andrei V Gudkov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Illinois, Andrei V Gudkov filed Critical Univ Illinois
Priority to AU2002365031A priority Critical patent/AU2002365031A1/en
Publication of WO2003050501A2 publication Critical patent/WO2003050501A2/en
Publication of WO2003050501A3 publication Critical patent/WO2003050501A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention describes a general method of isolation genetic elements, encoding bioproducts that exert their biological activity from the outside the target cell. The method allows the effective identification of the desired gene products (and the factors induced by the activity of such products in target cells) that can act from the outside of the cells and, therefore, do not require additional delivery systems for their in vivo applications.
PCT/US2002/025261 2001-08-10 2002-08-08 Indirect fluctuation screening WO2003050501A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002365031A AU2002365031A1 (en) 2001-08-10 2002-08-08 Indirect fluctuation screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31166001P 2001-08-10 2001-08-10
US60/311,660 2001-08-10

Publications (2)

Publication Number Publication Date
WO2003050501A2 WO2003050501A2 (en) 2003-06-19
WO2003050501A3 true WO2003050501A3 (en) 2004-02-26

Family

ID=23207894

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/025261 WO2003050501A2 (en) 2001-08-10 2002-08-08 Indirect fluctuation screening

Country Status (2)

Country Link
AU (1) AU2002365031A1 (en)
WO (1) WO2003050501A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018138271A1 (en) * 2017-01-26 2018-08-02 Biomillenia Sas High-throughput microfluidic screening in droplets

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995023855A2 (en) * 1994-03-02 1995-09-08 Board Of Trustees Of The University Of Illinois Genes and genetic elements associated with control of neoplastic transformation in mammalian cells
US5665550A (en) * 1990-10-19 1997-09-09 Board Of Trustees Of The University Of Illinois-Urbana Genes and genetic elements associated with sensitivity to chemotherapeutic drugs
US6326488B1 (en) * 1990-10-19 2001-12-04 Board Of Trustees Of University Of Illinois Gene and genetic elements associated with sensitivity to chemotherapeutic drugs

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665550A (en) * 1990-10-19 1997-09-09 Board Of Trustees Of The University Of Illinois-Urbana Genes and genetic elements associated with sensitivity to chemotherapeutic drugs
US6326488B1 (en) * 1990-10-19 2001-12-04 Board Of Trustees Of University Of Illinois Gene and genetic elements associated with sensitivity to chemotherapeutic drugs
WO1995023855A2 (en) * 1994-03-02 1995-09-08 Board Of Trustees Of The University Of Illinois Genes and genetic elements associated with control of neoplastic transformation in mammalian cells

Also Published As

Publication number Publication date
WO2003050501A2 (en) 2003-06-19
AU2002365031A1 (en) 2003-06-23
AU2002365031A8 (en) 2003-06-23

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