WO2003048783A3 - Monocyte adhesion - Google Patents

Monocyte adhesion Download PDF

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Publication number
WO2003048783A3
WO2003048783A3 PCT/GB2002/005223 GB0205223W WO03048783A3 WO 2003048783 A3 WO2003048783 A3 WO 2003048783A3 GB 0205223 W GB0205223 W GB 0205223W WO 03048783 A3 WO03048783 A3 WO 03048783A3
Authority
WO
WIPO (PCT)
Prior art keywords
monocytes
cell line
solid phase
quantitating
hsp
Prior art date
Application number
PCT/GB2002/005223
Other languages
French (fr)
Other versions
WO2003048783A2 (en
Inventor
Robin Poston
Original Assignee
Robin Poston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robin Poston filed Critical Robin Poston
Priority to AU2002343048A priority Critical patent/AU2002343048A1/en
Publication of WO2003048783A2 publication Critical patent/WO2003048783A2/en
Publication of WO2003048783A3 publication Critical patent/WO2003048783A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method of assaying leukocyte binding to solid phase heat shock protein (HSP) which comprises contacting HSP on a solid support with a suspension in a suitable medium of monocytes or a cell line having adhesion properties similar to monocytes and quantitating the number of cells bound to the support. The invention also relates to a method of assaying leukocyte binding via the CD14/TLR signalling complex to a target ligand which comprises contacting the ligand on a solid phase with a suspension in a suitable medium of monocytes or a cell line having adhesion properties similar to monocytes or a cell line transfected with complex components and quantitating the number of cells bound to the solid phase. Agents identified as inhibitors in these assays may be useful in the treatment or prevention of atherosclerosis or related clinical disease and/or for the treatment of conditions where stressed cells in a diseased tissue are targeted by chronic inflammatory responses.
PCT/GB2002/005223 2001-11-30 2002-11-21 Monocyte adhesion WO2003048783A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002343048A AU2002343048A1 (en) 2001-11-30 2002-11-21 Monocyte adhesion

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0128712.7 2001-11-30
GB0128712A GB0128712D0 (en) 2001-11-30 2001-11-30 Monocyte adhesion

Publications (2)

Publication Number Publication Date
WO2003048783A2 WO2003048783A2 (en) 2003-06-12
WO2003048783A3 true WO2003048783A3 (en) 2004-02-26

Family

ID=9926760

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/005223 WO2003048783A2 (en) 2001-11-30 2002-11-21 Monocyte adhesion

Country Status (3)

Country Link
AU (1) AU2002343048A1 (en)
GB (1) GB0128712D0 (en)
WO (1) WO2003048783A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058949A1 (en) * 2003-12-19 2005-06-30 Agency For Science, Technology And Research Protein separation device
ES2379113T3 (en) * 2006-05-17 2012-04-20 Eberhard-Karls-Universität Tübingen Universitätsklinikum Anti-inflammatory fusion protein

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994029720A1 (en) * 1993-06-15 1994-12-22 Robin Poston Leukocyte adhesion assay
WO2001065257A2 (en) * 2000-03-03 2001-09-07 Gerd Schmitz Method for the analysis of exogenic and endogenic cell activation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994029720A1 (en) * 1993-06-15 1994-12-22 Robin Poston Leukocyte adhesion assay
WO2001065257A2 (en) * 2000-03-03 2001-09-07 Gerd Schmitz Method for the analysis of exogenic and endogenic cell activation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KOL A ET AL: "Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cells.", JOURNAL OF IMMUNOLOGY (BALTIMORE, MD.: 1950) UNITED STATES 1 JAN 2000, vol. 164, no. 1, 1 January 2000 (2000-01-01), pages 13 - 17, XP002262385, ISSN: 0022-1767 *

Also Published As

Publication number Publication date
WO2003048783A2 (en) 2003-06-12
AU2002343048A8 (en) 2003-06-17
GB0128712D0 (en) 2002-01-23
AU2002343048A1 (en) 2003-06-17

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