WO2003044533A3 - Capture and detection phase of a target biological material for elisa tests - Google Patents

Capture and detection phase of a target biological material for elisa tests Download PDF

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Publication number
WO2003044533A3
WO2003044533A3 PCT/FR2002/003999 FR0203999W WO03044533A3 WO 2003044533 A3 WO2003044533 A3 WO 2003044533A3 FR 0203999 W FR0203999 W FR 0203999W WO 03044533 A3 WO03044533 A3 WO 03044533A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological material
target biological
phase
capture
hapten
Prior art date
Application number
PCT/FR2002/003999
Other languages
French (fr)
Other versions
WO2003044533A2 (en
Inventor
Francois Mallet
Thierry Delair
Valerie Cheynet
Guy Oriol
Laurent Veron
Marie-Helene Charles
Laure Allard
Original Assignee
Biomerieux Sa
Francois Mallet
Thierry Delair
Valerie Cheynet
Guy Oriol
Laurent Veron
Marie-Helene Charles
Laure Allard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux Sa, Francois Mallet, Thierry Delair, Valerie Cheynet, Guy Oriol, Laurent Veron, Marie-Helene Charles, Laure Allard filed Critical Biomerieux Sa
Priority to AU2002356260A priority Critical patent/AU2002356260A1/en
Publication of WO2003044533A2 publication Critical patent/WO2003044533A2/en
Publication of WO2003044533A3 publication Critical patent/WO2003044533A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention concerns a method for obtaining a capture phase of a target biological material, comprising a modified protein of interest, capable of binding with said target biological material and immobilized on an immobilization phase which consists in providing at least two different peptide sequences, including the peptide sequence of the protein of interest, and a succession of at least six lysine residues and a succession of at least six histidine residues at their N- and C- termini, immobilizing each of the two peptide sequences obtained on the immobilization phase, determining the efficacy of the coupling between each of the two sequences and the immobilization phase, selecting as capture phase, the peptide sequence more efficiently coupled to the immobilization phase. The invention also concerns a method for obtaining a detection phase of a target biological material, comprising a modified protein of interest capable of binding with said target biological material and coupled to a hapten, which consists in providing at least two different peptide sequences, including the peptide sequence of the protein of interest, and a succession of at least six lysine residues and a succession of at least six histidine residues at their N- and C- termini, coupling each of the two peptide sequences obtained to the hapten, determining the efficacy of the coupling between each of the two sequences to the hapten, selecting as detection phase, the peptide sequence more efficiently coupled to the hapten, and the use of the capture and detection phases obtained for detecting a target biological material, in ELISA.
PCT/FR2002/003999 2001-11-21 2002-11-21 Capture and detection phase of a target biological material for elisa tests WO2003044533A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002356260A AU2002356260A1 (en) 2001-11-21 2002-11-21 Capture and detection phase of a target biological material for elisa tests

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR01/15087 2001-11-21
FR0115087A FR2832508A1 (en) 2001-11-21 2001-11-21 Preparing selective capture and detection phases, useful in immunological assay of biomolecules, using binding molecules modified by polyhistidine and polylysine tags

Publications (2)

Publication Number Publication Date
WO2003044533A2 WO2003044533A2 (en) 2003-05-30
WO2003044533A3 true WO2003044533A3 (en) 2004-02-26

Family

ID=8869649

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/003999 WO2003044533A2 (en) 2001-11-21 2002-11-21 Capture and detection phase of a target biological material for elisa tests

Country Status (3)

Country Link
AU (1) AU2002356260A1 (en)
FR (1) FR2832508A1 (en)
WO (1) WO2003044533A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113588833B (en) * 2021-08-04 2023-04-28 谱天（天津）生物科技有限公司 Reference for monitoring proteolysis efficiency and miscut rate in proteome sample preparation and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0319012A2 (en) * 1987-12-04 1989-06-07 The Du Pont Merck Pharmaceutical Company Immobilized interleukin 2 and interleukin 2 containing a carboxyl-terminal extension
US5723344A (en) * 1993-06-25 1998-03-03 Bio Merieux Device for the capture of target molecules, and capturing process using the device
FR2764988A1 (en) * 1997-06-20 1998-12-24 Bio Merieux CONJUGATED COMPOUND, REAGENT AND KIT CONTAINING THE SAME, AND USES IN A METHOD OF FIXING BIOLOGICAL MATERIAL

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0319012A2 (en) * 1987-12-04 1989-06-07 The Du Pont Merck Pharmaceutical Company Immobilized interleukin 2 and interleukin 2 containing a carboxyl-terminal extension
US5723344A (en) * 1993-06-25 1998-03-03 Bio Merieux Device for the capture of target molecules, and capturing process using the device
FR2764988A1 (en) * 1997-06-20 1998-12-24 Bio Merieux CONJUGATED COMPOUND, REAGENT AND KIT CONTAINING THE SAME, AND USES IN A METHOD OF FIXING BIOLOGICAL MATERIAL

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG T ET AL: "COVALENT IMMOBLIZATION OF IMMUNOGLOBULIN TO POLYSTYRENE AND SILICONNITRIDE CHIPS", AMERICAN CHEMICAL SOCIETY. ABSTRACTS OF PAPER. AT THE NATIONAL MEETING, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 192, 7 September 1986 (1986-09-07), pages ABSTRNO13, XP000563391, ISSN: 0065-7727 *

Also Published As

Publication number Publication date
FR2832508A1 (en) 2003-05-23
WO2003044533A2 (en) 2003-05-30
AU2002356260A1 (en) 2003-06-10
AU2002356260A8 (en) 2003-06-10

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