WO2003042658A3 - Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents - Google Patents

Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents Download PDF

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Publication number
WO2003042658A3
WO2003042658A3 PCT/US2002/035893 US0235893W WO03042658A3 WO 2003042658 A3 WO2003042658 A3 WO 2003042658A3 US 0235893 W US0235893 W US 0235893W WO 03042658 A3 WO03042658 A3 WO 03042658A3
Authority
WO
WIPO (PCT)
Prior art keywords
probes
target
tag
detection
compositions
Prior art date
Application number
PCT/US2002/035893
Other languages
French (fr)
Other versions
WO2003042658A2 (en
Inventor
Ahmed Chenna
Sharat Singh
Original Assignee
Aclara Biosciences Inc
Ahmed Chenna
Sharat Singh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/011,201 external-priority patent/US7037654B2/en
Application filed by Aclara Biosciences Inc, Ahmed Chenna, Sharat Singh filed Critical Aclara Biosciences Inc
Priority to US10/494,879 priority Critical patent/US20050053939A1/en
Priority to AU2002363725A priority patent/AU2002363725A1/en
Publication of WO2003042658A2 publication Critical patent/WO2003042658A2/en
Publication of WO2003042658A3 publication Critical patent/WO2003042658A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals

Abstract

Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region, both linked to a target-binding moiety. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Also described are biopolymers and nucleosides containing such interactive functionalities.
PCT/US2002/035893 2001-11-09 2002-11-08 Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents WO2003042658A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/494,879 US20050053939A1 (en) 2001-11-09 2002-11-08 Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
AU2002363725A AU2002363725A1 (en) 2001-11-09 2002-11-08 Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33798201P 2001-11-09 2001-11-09
US10/011,201 2001-11-09
US60/337,982 2001-11-09
US10/011,201 US7037654B2 (en) 1999-04-30 2001-11-09 Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents

Publications (2)

Publication Number Publication Date
WO2003042658A2 WO2003042658A2 (en) 2003-05-22
WO2003042658A3 true WO2003042658A3 (en) 2003-12-04

Family

ID=29586142

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/035893 WO2003042658A2 (en) 2001-11-09 2002-11-08 Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents

Country Status (2)

Country Link
AU (1) AU2002363725A1 (en)
WO (1) WO2003042658A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes
JP3929949B2 (en) * 2003-08-08 2007-06-13 独立行政法人科学技術振興機構 Nucleoside releasing functional unit by oxidation and method for producing oligonucleotide containing the same
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US10416162B2 (en) 2007-12-20 2019-09-17 Monogram Biosciences, Inc. Her2 diagnostic methods
WO2010065568A2 (en) 2008-12-01 2010-06-10 Laboratory Corporation Of America Holdings METHODS AND ASSAYS FOR MEASURING p95 AND/OR p95 IN A SAMPLE AND ANTIBODIES SPECIFIC FOR p95
ES2535723T3 (en) 2009-01-15 2015-05-14 Laboratory Corporation Of America Holdings Methods for determining the patient's response by measuring Her-3
EP3403095B1 (en) 2016-03-15 2019-08-07 Laboratory Corporation of America Holdings Methods of assessing protein interactions between cells
WO2019006455A1 (en) 2017-06-30 2019-01-03 Solstice Biologics, Ltd. Chiral phosphoramidite auxiliaries and methods of their use

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470705A (en) * 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470705A (en) * 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K

Also Published As

Publication number Publication date
AU2002363725A1 (en) 2003-05-26
WO2003042658A2 (en) 2003-05-22

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