WO2003033096A3 - Verfahren und trennmodul zum abtrennen von partikeln aus einer dispersion, insbesondere von blutkörperchen aus blut - Google Patents

Verfahren und trennmodul zum abtrennen von partikeln aus einer dispersion, insbesondere von blutkörperchen aus blut Download PDF

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Publication number
WO2003033096A3
WO2003033096A3 PCT/EP2002/010336 EP0210336W WO03033096A3 WO 2003033096 A3 WO2003033096 A3 WO 2003033096A3 EP 0210336 W EP0210336 W EP 0210336W WO 03033096 A3 WO03033096 A3 WO 03033096A3
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WO
WIPO (PCT)
Prior art keywords
blood
dispersion
particles
separation
branching
Prior art date
Application number
PCT/EP2002/010336
Other languages
English (en)
French (fr)
Other versions
WO2003033096A2 (de
Inventor
Carlo Effenhauser
Gregor Ocvirk
Wolfgang Fiedler
Original Assignee
Roche Diagnostics Gmbh
Hoffmann La Roche
Carlo Effenhauser
Gregor Ocvirk
Wolfgang Fiedler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Hoffmann La Roche, Carlo Effenhauser, Gregor Ocvirk, Wolfgang Fiedler filed Critical Roche Diagnostics Gmbh
Priority to EP02774618A priority Critical patent/EP1434637B1/de
Priority to DE50205804T priority patent/DE50205804D1/de
Priority to JP2003535890A priority patent/JP4005022B2/ja
Priority to CA002463223A priority patent/CA2463223C/en
Priority to AU2002340906A priority patent/AU2002340906A1/en
Priority to US10/492,256 priority patent/US7527740B2/en
Publication of WO2003033096A2 publication Critical patent/WO2003033096A2/de
Publication of WO2003033096A3 publication Critical patent/WO2003033096A3/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/05—Investigating sedimentation of particle suspensions in blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/05—Investigating sedimentation of particle suspensions in blood
    • G01N2015/055—Investigating sedimentation of particle suspensions in blood for hematocrite determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Vascular Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Dispersion Chemistry (AREA)
  • Anesthesiology (AREA)
  • Urology & Nephrology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Cardiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Verfahren zum Abtrennen von Partikeln aus einer flüssigen Dispersion, insbesondere zum Abtrennen von korpuskulären Bestandteilen aus biologischen Proben, vor allem aus Blut. Ein zur Durchführung des Verfahrens geeignetes Trennmodul weist ein Substrat (5) mit Strömungskanälen (3), umfassend einen Zuflusskanal (9) zum Zuführen der Dispersion zu einer Verzweigung (10), einen ersten Abflusskanal (11) zum Ableiten von Fluid mit verminderter Partikelkonzentration von der Verzweigung (10) weg and einen zweiten Abflusskanal (12) zum Ableiten von Fluid mit erhöhter Partikelkonzentration von der Verzweigung (10) weg, auf. Das Fluid strömt in dem zweiten Abflusskanal so viel schneller als in dem ersten Abflusskanal (12), dass die dispergierten Partikel an der Verzweigung (10) aufgrund der unterschiedlichen Strömungsgeschwindigkeit bevorzugt in dem zweiten Abflusskanal (12) weiterströmen.
PCT/EP2002/010336 2001-10-12 2002-09-14 Verfahren und trennmodul zum abtrennen von partikeln aus einer dispersion, insbesondere von blutkörperchen aus blut WO2003033096A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP02774618A EP1434637B1 (de) 2001-10-12 2002-09-14 Verfahren und trennmodul zum abtrennen von partikeln aus einer dispersion, insbesondere von blutkörperchen aus blut
DE50205804T DE50205804D1 (de) 2001-10-12 2002-09-14 Verfahren und trennmodul zum abtrennen von partikeln aus einer dispersion, insbesondere von blutkörperchen aus blut
JP2003535890A JP4005022B2 (ja) 2001-10-12 2002-09-14 分散体からの粒子、とくに血液からの血球の分離方法および分離モジュール
CA002463223A CA2463223C (en) 2001-10-12 2002-09-14 Method and separating module for separating particles from a dispersion, particularly blood corpuscles from blood
AU2002340906A AU2002340906A1 (en) 2001-10-12 2002-09-14 Method and separating module for the separation of particles from a dispersion, in particular of blood corpuscles from blood
US10/492,256 US7527740B2 (en) 2001-10-12 2002-09-14 Method and separating module for the separation of particles from a dispersion, in particular of blood corpuscles from blood

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10150549A DE10150549A1 (de) 2001-10-12 2001-10-12 Verfahren und Trennmodul zum Abtrennen von Partikeln aus einer Dispersion, insbesondere von Blutkörperchen aus Blut
DE10150549.3 2001-10-12

Publications (2)

Publication Number Publication Date
WO2003033096A2 WO2003033096A2 (de) 2003-04-24
WO2003033096A3 true WO2003033096A3 (de) 2003-12-18

Family

ID=7702370

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/010336 WO2003033096A2 (de) 2001-10-12 2002-09-14 Verfahren und trennmodul zum abtrennen von partikeln aus einer dispersion, insbesondere von blutkörperchen aus blut

Country Status (9)

Country Link
US (1) US7527740B2 (de)
EP (1) EP1434637B1 (de)
JP (1) JP4005022B2 (de)
AT (1) ATE317281T1 (de)
AU (1) AU2002340906A1 (de)
CA (1) CA2463223C (de)
DE (2) DE10150549A1 (de)
ES (1) ES2257574T3 (de)
WO (1) WO2003033096A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10313201A1 (de) * 2003-03-21 2004-10-07 Steag Microparts Gmbh Mikrostrukturierte Trennvorrichtung und mikrofluidisches Verfahren zum Abtrennen von flüssigen Bestandteilen aus einer Flüssigkeit, die Partikel enthält
TW200702292A (en) * 2005-02-28 2007-01-16 Careside Medical Llc A micro-fluidic fluid separation device and method
JP2007021465A (ja) * 2005-07-12 2007-02-01 Minoru Seki 粒子を連続的に濃縮・分離するための流路構造および方法
DE102005050167B4 (de) * 2005-10-19 2009-02-19 Advalytix Ag Konzentrationsverfahren, Konzentrationsvorrichtung und Reaktionsverfahren
EP2020598A4 (de) * 2006-05-24 2012-12-19 Univ Kyoto Mikrokanal zur trennung von blutplasma
US8075468B2 (en) * 2008-02-27 2011-12-13 Fenwal, Inc. Systems and methods for mid-processing calculation of blood composition
US8685258B2 (en) * 2008-02-27 2014-04-01 Fenwal, Inc. Systems and methods for conveying multiple blood components to a recipient
CL2009000671A1 (es) * 2008-03-19 2011-02-18 Ocnosis Pharma Aie Dispositivo para separar particulas en una dispersion de fluido, que comprende un canal de entrada y un canal de salida contiguos entre si a traves de un canal de contricion, presentando una primera y una segunda zona de flujo con una concentracion de particulas inferior que la primera, y un canal de recogida; y procedimiento para separar particulas.
JP2010071857A (ja) * 2008-09-19 2010-04-02 Sekisui Chem Co Ltd 血漿分離装置
US8980106B2 (en) 2010-12-15 2015-03-17 Abbott Laboratories Apparatus and method for separation of whole blood into plasma or serum and cells
DE102012206371A1 (de) * 2012-04-18 2013-10-24 Siemens Ag Durchflusssystem mit einer Rückhaltestruktur für Partikel und dessen Verwendung
WO2014128960A1 (ja) * 2013-02-25 2014-08-28 株式会社メニコン マイクロ流体デバイス

Citations (4)

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EP0057907A1 (de) * 1981-02-05 1982-08-18 Asahi Kasei Kogyo Kabushiki Kaisha Vorrichtung zur Trennung von Blutkomponenten
JPS60201253A (ja) * 1984-03-26 1985-10-11 Jeol Ltd 血漿採取装置
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JPS60201253A (ja) * 1984-03-26 1985-10-11 Jeol Ltd 血漿採取装置
US5922210A (en) * 1995-06-16 1999-07-13 University Of Washington Tangential flow planar microfabricated fluid filter and method of using thereof
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Also Published As

Publication number Publication date
EP1434637A2 (de) 2004-07-07
US7527740B2 (en) 2009-05-05
JP4005022B2 (ja) 2007-11-07
ATE317281T1 (de) 2006-02-15
CA2463223A1 (en) 2003-04-24
US20050029190A1 (en) 2005-02-10
CA2463223C (en) 2009-08-25
AU2002340906A1 (en) 2003-04-28
DE10150549A1 (de) 2003-04-17
DE50205804D1 (de) 2006-04-20
ES2257574T3 (es) 2006-08-01
JP2005505770A (ja) 2005-02-24
WO2003033096A2 (de) 2003-04-24
EP1434637B1 (de) 2006-02-08

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