WO2003029485A3 - Specific differential display arrays - Google Patents

Specific differential display arrays Download PDF

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Publication number
WO2003029485A3
WO2003029485A3 PCT/DK2002/000653 DK0200653W WO03029485A3 WO 2003029485 A3 WO2003029485 A3 WO 2003029485A3 DK 0200653 W DK0200653 W DK 0200653W WO 03029485 A3 WO03029485 A3 WO 03029485A3
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WO
WIPO (PCT)
Prior art keywords
differential display
specific differential
display arrays
stressed
identifying
Prior art date
Application number
PCT/DK2002/000653
Other languages
French (fr)
Other versions
WO2003029485A2 (en
Inventor
Jens Bitsch-Noerhave
Kenneth Thirstrup
Rene Hummel
Original Assignee
Azign Bioscience As
Jens Bitsch-Noerhave
Kenneth Thirstrup
Rene Hummel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Azign Bioscience As, Jens Bitsch-Noerhave, Kenneth Thirstrup, Rene Hummel filed Critical Azign Bioscience As
Priority to EP02800000A priority Critical patent/EP1434883A2/en
Publication of WO2003029485A2 publication Critical patent/WO2003029485A2/en
Publication of WO2003029485A3 publication Critical patent/WO2003029485A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for identifying a gene fragment that is differentially expressed in a non-stressed and stressed state of a biological source, said method comprising i) identifying a gene that is differentially expressed in a non-stressed and stressed state, and ii) identifying a fragment of the identified gene, said fragment comprising a non-conserved region.
PCT/DK2002/000653 2001-10-02 2002-10-02 Specific differential display arrays WO2003029485A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02800000A EP1434883A2 (en) 2001-10-02 2002-10-02 Specific differential display arrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101438 2001-10-02
DKPA200101438 2001-10-02

Publications (2)

Publication Number Publication Date
WO2003029485A2 WO2003029485A2 (en) 2003-04-10
WO2003029485A3 true WO2003029485A3 (en) 2004-03-04

Family

ID=8160744

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000653 WO2003029485A2 (en) 2001-10-02 2002-10-02 Specific differential display arrays

Country Status (2)

Country Link
EP (1) EP1434883A2 (en)
WO (1) WO2003029485A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946177B2 (en) 2004-11-12 2015-02-03 Mima Therapeutics, Inc Methods and compositions involving miRNA and miRNA inhibitor molecules

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EP2290074B1 (en) 2004-05-28 2014-12-17 Asuragen, Inc. Methods and compositions involving microRNA
AU2007299828C1 (en) 2006-09-19 2014-07-17 Interpace Diagnostics, Llc MicroRNAs differentially expressed in pancreatic diseases and uses thereof
CA2663962A1 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir-31,mir-145, mir-147, mir-188, mir-215, mir-216, mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
EP2198050A1 (en) 2007-09-14 2010-06-23 Asuragen, INC. Micrornas differentially expressed in cervical cancer and uses thereof
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
WO2011108930A1 (en) 2010-03-04 2011-09-09 Interna Technologies Bv A MiRNA MOLECULE DEFINED BY ITS SOURCE AND ITS DIAGNOSTIC AND THERAPEUTIC USES IN DISEASES OR CONDITIONS ASSOCIATED WITH EMT
WO2012005572A1 (en) 2010-07-06 2012-01-12 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
AU2011329772B2 (en) 2010-11-17 2017-05-04 Interpace Diagnostics, Llc miRNAs as biomarkers for distinguishing benign from malignant thyroid neoplasms
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
US20140057295A1 (en) 2011-02-28 2014-02-27 Barbara J. Stegmann Anti-mullerian hormone changes in pregnancy and prediction of adverse pregnancy outcomes and gender
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
US20130157884A1 (en) 2011-10-26 2013-06-20 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
WO2013063544A1 (en) 2011-10-27 2013-05-02 Asuragen, Inc. Mirnas as diagnostic biomarkers to distinguish benign from malignant thyroid tumors
EP2870263A1 (en) 2012-07-03 2015-05-13 InteRNA Technologies B.V. Diagnostic portfolio and its uses
US20140100124A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
CA2907377A1 (en) 2013-03-15 2014-09-18 Baylor Research Institute Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
ES2935257T3 (en) 2013-03-15 2023-03-03 Univ Chicago Methods and Compositions Related to T Cell Activity
WO2014151551A1 (en) 2013-03-15 2014-09-25 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
EP3704250A1 (en) 2017-11-03 2020-09-09 InteRNA Technologies B.V. Mirna molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
CN108663462B (en) * 2018-08-06 2021-04-13 通标标准技术服务(上海)有限公司 Method for measuring vitamin A, D and E in milk powder
KR20210151944A (en) 2019-04-12 2021-12-14 더 리전트 오브 더 유니버시티 오브 캘리포니아 Compositions and methods for increasing muscle mass and oxidative metabolism
WO2024028794A1 (en) 2022-08-02 2024-02-08 Temple Therapeutics BV Methods for treating endometrial and ovarian hyperproliferative disorders

Citations (8)

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Publication number Priority date Publication date Assignee Title
WO1998051789A2 (en) * 1997-05-13 1998-11-19 Display Systems Biotech A/S A METHOD TO CLONE mRNAs AND DISPLAY OF DIFFERENTIALLY EXPRESSED TRANSCRIPTS (DODET)
WO1999049894A1 (en) * 1998-04-01 1999-10-07 Genentech, Inc. Antagonists to growth arrest specific gene 6 to treat insulin-resistant disorders
WO1999055913A2 (en) * 1998-04-27 1999-11-04 Sidney Kimmel Cancer Center Reduced complexity nucleic acid targets and methods of using same
WO2000000646A1 (en) * 1998-06-30 2000-01-06 The Scripps Research Institute METHOD FOR SIMULTANEOUS IDENTIFICATION OF DIFFERENTIALLY EXPRESSED mRNAs AND MEASUREMENT OF RELATIVE CONCENTRATIONS
GB2350676A (en) * 1999-06-01 2000-12-06 Proteome Sciences Plc Method of screening for an agent for treating insulin-related disorders
WO2001002560A1 (en) * 1999-06-29 2001-01-11 Autogen Research Pty. Ltd. Novel genes and their use in the modulation of obesity, diabetes and energy imbalance
WO2001063295A2 (en) * 2000-02-24 2001-08-30 Oxford Glycosciences (Uk) Ltd. Dpi-6, a therapeutic biomarker in neurological disorders
WO2002095064A1 (en) * 2001-05-18 2002-11-28 Azign Bioscience A/S Transporter arrays and methods and kits for producing and using the arrays

Patent Citations (8)

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Publication number Priority date Publication date Assignee Title
WO1998051789A2 (en) * 1997-05-13 1998-11-19 Display Systems Biotech A/S A METHOD TO CLONE mRNAs AND DISPLAY OF DIFFERENTIALLY EXPRESSED TRANSCRIPTS (DODET)
WO1999049894A1 (en) * 1998-04-01 1999-10-07 Genentech, Inc. Antagonists to growth arrest specific gene 6 to treat insulin-resistant disorders
WO1999055913A2 (en) * 1998-04-27 1999-11-04 Sidney Kimmel Cancer Center Reduced complexity nucleic acid targets and methods of using same
WO2000000646A1 (en) * 1998-06-30 2000-01-06 The Scripps Research Institute METHOD FOR SIMULTANEOUS IDENTIFICATION OF DIFFERENTIALLY EXPRESSED mRNAs AND MEASUREMENT OF RELATIVE CONCENTRATIONS
GB2350676A (en) * 1999-06-01 2000-12-06 Proteome Sciences Plc Method of screening for an agent for treating insulin-related disorders
WO2001002560A1 (en) * 1999-06-29 2001-01-11 Autogen Research Pty. Ltd. Novel genes and their use in the modulation of obesity, diabetes and energy imbalance
WO2001063295A2 (en) * 2000-02-24 2001-08-30 Oxford Glycosciences (Uk) Ltd. Dpi-6, a therapeutic biomarker in neurological disorders
WO2002095064A1 (en) * 2001-05-18 2002-11-28 Azign Bioscience A/S Transporter arrays and methods and kits for producing and using the arrays

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FRED REGNIER ET AL: "Multidimensional Chromatography and the Signature Peptide Approach to Proteomics", LCGC, vol. 19, no. 2, February 2001 (2001-02-01), pages 200 - 213, XP002232798, Retrieved from the Internet <URL:http:www.chromatographyonline.com> [retrieved on 20030226] *
GYGI STEVEN P ET AL: "Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.", NATURE BIOTECHNOLOGY, vol. 17, no. 10, October 1999 (1999-10-01), pages 994 - 999, XP002232658, ISSN: 1087-0156 *
YAMANO MAYUMI ET AL: "Corticotropin-releasing hormone1 receptors mediate consensus interferon-alpha YM643-induced depression-like behavior in mice.", JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 292, no. 1, January 2000 (2000-01-01), pages 181 - 187, XP002232660, ISSN: 0022-3565 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8946177B2 (en) 2004-11-12 2015-02-03 Mima Therapeutics, Inc Methods and compositions involving miRNA and miRNA inhibitor molecules

Also Published As

Publication number Publication date
EP1434883A2 (en) 2004-07-07
WO2003029485A2 (en) 2003-04-10

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