WO2003029425A3 - Whole cell engineering using real-time metabolic flux analysis - Google Patents

Whole cell engineering using real-time metabolic flux analysis Download PDF

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Publication number
WO2003029425A3
WO2003029425A3 PCT/US2002/031380 US0231380W WO03029425A3 WO 2003029425 A3 WO2003029425 A3 WO 2003029425A3 US 0231380 W US0231380 W US 0231380W WO 03029425 A3 WO03029425 A3 WO 03029425A3
Authority
WO
WIPO (PCT)
Prior art keywords
real
cell
modified
flux analysis
metabolic flux
Prior art date
Application number
PCT/US2002/031380
Other languages
French (fr)
Other versions
WO2003029425A2 (en
Inventor
Pencheng Fu
Jay M Short
Original Assignee
Diversa Corp
Pencheng Fu
Jay M Short
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp, Pencheng Fu, Jay M Short filed Critical Diversa Corp
Priority to CA002462641A priority Critical patent/CA2462641A1/en
Priority to JP2003532643A priority patent/JP2005506840A/en
Priority to DE2002786364 priority patent/DE02786364T1/en
Priority to US10/491,358 priority patent/US20050202426A1/en
Priority to EP02786364A priority patent/EP1446495A4/en
Publication of WO2003029425A2 publication Critical patent/WO2003029425A2/en
Publication of WO2003029425A3 publication Critical patent/WO2003029425A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Abstract

The invention provides methods for whole cell engineering of new and modified phenotypes by using 'on-line' or 'real-time' metabolic flux analysis. The invention provides a method for whole cell engineering of new or modified phenotypes by using real-time metabolic flux analysis by making a modified cell by modifying the genetic composition of a cell and culturing the modified cell to generate a plurality of modified cells and measuring at least one metabolic parameter of the cell by monitoring the cell culture of in real time. The invention also provides articles comprising machine-readable medium including machine-executable instructions and systems, e.g., computer systems, to practice the methods of the invention.
PCT/US2002/031380 2001-10-01 2002-10-01 Whole cell engineering using real-time metabolic flux analysis WO2003029425A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002462641A CA2462641A1 (en) 2001-10-01 2002-10-01 Whole cell engineering using real-time metabolic flux analysis
JP2003532643A JP2005506840A (en) 2001-10-01 2002-10-01 Whole cell manipulation using real-time metabolic flux analysis
DE2002786364 DE02786364T1 (en) 2001-10-01 2002-10-01 CONSTRUCTION OF WHOLE CELLS USING A REAL-TIME ANALYSIS OF THE METABOLIC RIVER
US10/491,358 US20050202426A1 (en) 2001-10-01 2002-10-01 Whole cell engineering using real-time metabolic flux analysis
EP02786364A EP1446495A4 (en) 2001-10-01 2002-10-01 Whole cell engineering using real-time metabolic flux analysis

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US32665301P 2001-10-01 2001-10-01
US32665501P 2001-10-01 2001-10-01
US32665401P 2001-10-01 2001-10-01
US60/326,653 2001-10-01
US60/326,655 2001-10-01
US60/326,654 2001-10-01
US33752601P 2001-11-09 2001-11-09
US60/337,526 2001-11-09
US32665402P 2002-10-01 2002-10-01
US32665502P 2002-10-01 2002-10-01
US32665302P 2002-10-01 2002-10-01

Publications (2)

Publication Number Publication Date
WO2003029425A2 WO2003029425A2 (en) 2003-04-10
WO2003029425A3 true WO2003029425A3 (en) 2003-08-21

Family

ID=27739537

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/031380 WO2003029425A2 (en) 2001-10-01 2002-10-01 Whole cell engineering using real-time metabolic flux analysis

Country Status (1)

Country Link
WO (1) WO2003029425A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4894134B2 (en) * 2003-07-29 2012-03-14 味の素株式会社 Methods for determining metabolic fluxes that affect substance production
EP1705482B1 (en) 2003-12-11 2017-09-27 Kazuhiro Imai Method of detection, separation and identification for expressed trace protein/peptide
JP4654592B2 (en) * 2004-04-02 2011-03-23 味の素株式会社 Determination of metabolic flux
US20090035807A1 (en) * 2007-07-31 2009-02-05 Wyeth Analysis of polypeptide production
JP5267491B2 (en) * 2010-03-15 2013-08-21 三菱化学株式会社 Sugar chain separation method, sample analysis method, liquid chromatography apparatus, sugar chain analysis method and sugar chain analysis apparatus
WO2011123479A1 (en) * 2010-03-29 2011-10-06 Academia Sinica Quantitative measurement of nano / micro particle endocytosis with cell mass spectrometry
WO2017040498A1 (en) * 2015-08-31 2017-03-09 Yale University Techniques of mass spectrometry for isotopomer analysis and ralated systems and methods
WO2019067558A1 (en) 2017-09-28 2019-04-04 Precision Fermentation, Inc. Methods, devices and computer program products for yeast performance monitoring in fermentation systems
CA3075591A1 (en) * 2017-09-29 2019-04-04 Unibio A/S Optimization of fermentation processes
CN110196288A (en) * 2018-02-26 2019-09-03 中国科学院上海生命科学研究院 A kind of foundation and application of dynamic Metabolic flux analysis technology
EP4298200A1 (en) 2021-02-24 2024-01-03 Precision Fermentation, Inc. Devices and methods for monitoring

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077708A (en) * 1997-07-18 2000-06-20 Collins; Paul C. Method of determining progenitor cell content of a hematopoietic cell culture
US6147055A (en) * 1994-11-28 2000-11-14 Vical Incorporated Cancer treatment method utilizing plasmids suitable for IL-2 expression
US6184440B1 (en) * 1997-07-27 2001-02-06 Yissum Research Development Company Of The Hebrew University Of Jerusalem Transgenic plants of altered morphology
US6248876B1 (en) * 1990-08-31 2001-06-19 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US6281412B1 (en) * 1995-03-27 2001-08-28 Suntory Limited Method for creating osmotic-pressure-tolerant plant

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6248876B1 (en) * 1990-08-31 2001-06-19 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US6147055A (en) * 1994-11-28 2000-11-14 Vical Incorporated Cancer treatment method utilizing plasmids suitable for IL-2 expression
US6281412B1 (en) * 1995-03-27 2001-08-28 Suntory Limited Method for creating osmotic-pressure-tolerant plant
US6077708A (en) * 1997-07-18 2000-06-20 Collins; Paul C. Method of determining progenitor cell content of a hematopoietic cell culture
US6184440B1 (en) * 1997-07-27 2001-02-06 Yissum Research Development Company Of The Hebrew University Of Jerusalem Transgenic plants of altered morphology

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1446495A4 *

Also Published As

Publication number Publication date
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