WO2003027640A3 - Polymorphism detection and separation - Google Patents

Polymorphism detection and separation Download PDF

Info

Publication number
WO2003027640A3
WO2003027640A3 PCT/US2002/031073 US0231073W WO03027640A3 WO 2003027640 A3 WO2003027640 A3 WO 2003027640A3 US 0231073 W US0231073 W US 0231073W WO 03027640 A3 WO03027640 A3 WO 03027640A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
separation
detection
polymorphism detection
permit
Prior art date
Application number
PCT/US2002/031073
Other languages
French (fr)
Other versions
WO2003027640A2 (en
Inventor
Eric B Kmiec
Michael C Rice
Original Assignee
Univ Delaware
Eric B Kmiec
Michael C Rice
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2002/009691 external-priority patent/WO2002079495A2/en
Application filed by Univ Delaware, Eric B Kmiec, Michael C Rice filed Critical Univ Delaware
Priority to AU2002341898A priority Critical patent/AU2002341898A2/en
Priority to EP02776053A priority patent/EP1446502A4/en
Publication of WO2003027640A2 publication Critical patent/WO2003027640A2/en
Publication of WO2003027640A3 publication Critical patent/WO2003027640A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and compositions for polymorphism detection and separation. The methods are readily multiplexed, can be adapted to a variety of existing detection systems, and permit target amplification without PCR. The methods permit allelic variants selectively to be isolated, with or without contemporaneous detection, and finds particular utility in facilitating the construction of coisogenic cell collections in which the cells differ genotypically by single nucleotide changes targeted to defined loci.
PCT/US2002/031073 2001-09-28 2002-09-27 Polymorphism detection and separation WO2003027640A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002341898A AU2002341898A2 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation
EP02776053A EP1446502A4 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32582801P 2001-09-28 2001-09-28
US60/325,828 2001-09-28
USPCT/US02/09691 2002-03-27
PCT/US2002/009691 WO2002079495A2 (en) 2001-03-27 2002-03-27 Genomics applications for modified oligonucleotides

Publications (2)

Publication Number Publication Date
WO2003027640A2 WO2003027640A2 (en) 2003-04-03
WO2003027640A3 true WO2003027640A3 (en) 2003-07-31

Family

ID=26680615

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/031073 WO2003027640A2 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation

Country Status (3)

Country Link
EP (1) EP1446502A4 (en)
AU (1) AU2002341898A2 (en)
WO (1) WO2003027640A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945845B2 (en) 2002-02-21 2015-02-03 Alere San Diego Inc. Recombinase polymerase amplification
US8962255B2 (en) 2002-02-21 2015-02-24 Alere San Diego, Inc. Recombinase polymerase amplification
US9340825B2 (en) 2002-02-21 2016-05-17 Alere San Diego, Inc. Compositions for recombinase polymerase amplification
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10353419B3 (en) * 2003-11-09 2005-06-30 Epigenomics Ag Method for studying cytosine methylations in DNA sequences using hemimethylation-sensitive restriction enzymes
EP2336361B1 (en) * 2004-06-01 2019-01-23 Alere San Diego, Inc. Kit for recombinase polymerase amplification
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US20100280084A1 (en) * 2005-09-12 2010-11-04 Government of the US, as represented by the Secret ary, Department of Health and Human Services Cyp1B1 Genotype
WO2008035205A2 (en) 2006-05-04 2008-03-27 Asm Scientific, Inc. Recombinase polymerase amplification
US9057097B2 (en) 2009-06-05 2015-06-16 Alere San Diego Inc. Recombinase polymerase amplification reagents and kits
WO2012138989A1 (en) 2011-04-07 2012-10-11 Alere San Diego Inc. Monitoring recombinase polymerase amplification mixtures
EP2992115B1 (en) * 2013-04-30 2020-03-04 California Institute of Technology Multiplex labeling of molecules by sequential hybridization barcoding

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2116215C (en) * 1990-05-07 1998-12-22 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double d-loop formation
US5929043A (en) * 1995-01-31 1999-07-27 Pangene Corporation Recombinase mediated DNA therapies
AU712873B2 (en) * 1996-08-29 1999-11-18 Tapestry Pharmaceuticals, Inc. Methods for targeting, enriching, detecting and/or isolating target nucleic acidsequence using reca-like recombinase
US20020061530A1 (en) * 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
CA2439098A1 (en) * 2001-02-21 2002-10-03 Gene Check, Inc. Mutation detection using muts and reca
WO2002079495A2 (en) * 2001-03-27 2002-10-10 University Of Delaware Genomics applications for modified oligonucleotides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1446502A4 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945845B2 (en) 2002-02-21 2015-02-03 Alere San Diego Inc. Recombinase polymerase amplification
US8962255B2 (en) 2002-02-21 2015-02-24 Alere San Diego, Inc. Recombinase polymerase amplification
US9309502B2 (en) 2002-02-21 2016-04-12 Alere San Diego Inc. Recombinase polymerase amplification
US9340825B2 (en) 2002-02-21 2016-05-17 Alere San Diego, Inc. Compositions for recombinase polymerase amplification
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates

Also Published As

Publication number Publication date
EP1446502A2 (en) 2004-08-18
EP1446502A4 (en) 2005-02-09
WO2003027640A2 (en) 2003-04-03
AU2002341898A2 (en) 2003-04-07

Similar Documents

Publication Publication Date Title
WO2003027640A3 (en) Polymorphism detection and separation
WO2006074222A3 (en) Primer for nucleic acid detection
WO2007070482A3 (en) Microarray-based preimplantation genetic diagnosis of chromosomal abnormalities
WO2002018639A3 (en) Detection of cyp2c19 polymorphisms
WO2007001986A3 (en) Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
AU2001245945A1 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
EP1350841A3 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
EP1791959A4 (en) Delivering functional nucleic acids to mammalian cells via bacterially derived, intact minicells
WO2001023583A3 (en) Thermostable enzyme promoting the fidelity of thermostable dna polymerases- for improvement of nucleic acid synthesis and amplification in vitro
EP1431303A3 (en) Composition for linear isothermal amplification of polynucleotide sequences
WO2006017134A3 (en) Preprimitive streak and mesendoderm cells
WO2005118853A3 (en) Recombinase polymerase amplification
WO2005035545A3 (en) Single nucleotide polymorphism analysis of highly polymorphic target sequences
AU1491401A (en) Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
WO2011070441A3 (en) Categorization of dna samples
WO2008064730A3 (en) Method for carrying out an enzymatic reaction
WO2006005064A3 (en) 5-methylcytosine detection, compositions and methods therefor
WO2001092569A3 (en) Formulation for polymerase chain reaction and vessel containing same
WO2009074882A3 (en) One-step target detection assay using a tagged and blocked target-specific pcr primer and an anti-tag capture complex
WO2007002031A3 (en) Dna polymerase blends and mutant dna polymerases
WO2004090132A3 (en) Method for isolating nucleic acids
WO2002034883A3 (en) A one-well assay for high throughput detection of single nucleotide polymorphisms
WO2004068112A3 (en) Hairpin primer amplification
WO2003071861A3 (en) Vanillin biosynthetic pathway enzyme from vanilla planifolia
WO2006039673A3 (en) Primers, methods and kits for amplifying or detecting human leukocyte antigen alleles

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BY BZ CA CH CN CO CR CU CZ DE DM DZ EC EE ES FI GB GD GE GH HR HU ID IL IN IS JP KE KG KP KR LC LK LR LS LT LU LV MA MD MG MN MW MX MZ NO NZ OM PH PL PT RU SD SE SG SI SK SL TJ TM TN TR TZ UA UG US UZ VC VN YU ZA ZM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ UG ZM ZW AM AZ BY KG KZ RU TJ TM AT BE BG CH CY CZ DK EE ES FI FR GB GR IE IT LU MC PT SE SK TR BF BJ CF CG CI GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002341898

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2002776053

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2002776053

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2002776053

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP