WO2003027640A3 - Polymorphism detection and separation - Google Patents

Polymorphism detection and separation Download PDF

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Publication number
WO2003027640A3
WO2003027640A3 PCT/US2002/031073 US0231073W WO03027640A3 WO 2003027640 A3 WO2003027640 A3 WO 2003027640A3 US 0231073 W US0231073 W US 0231073W WO 03027640 A3 WO03027640 A3 WO 03027640A3
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WO
Grant status
Application
Patent type
Prior art keywords
methods
separation
detection
polymorphism detection
permit
Prior art date
Application number
PCT/US2002/031073
Other languages
French (fr)
Other versions
WO2003027640A2 (en )
Inventor
Eric B Kmiec
Michael C Rice
Original Assignee
Eric B Kmiec
Michael C Rice
Univ Delaware
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means

Abstract

Methods and compositions for polymorphism detection and separation. The methods are readily multiplexed, can be adapted to a variety of existing detection systems, and permit target amplification without PCR. The methods permit allelic variants selectively to be isolated, with or without contemporaneous detection, and finds particular utility in facilitating the construction of coisogenic cell collections in which the cells differ genotypically by single nucleotide changes targeted to defined loci.
PCT/US2002/031073 2001-03-27 2002-09-27 Polymorphism detection and separation WO2003027640A3 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US32582801 true 2001-09-28 2001-09-28
US60/325,828 2001-09-28
PCT/US2002/009691 WO2002079495A3 (en) 2001-03-27 2002-03-27 Genomics applications for modified oligonucleotides
USPCT/US02/09691 2002-03-27

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20020776053 EP1446502A4 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation
AU2002341898A AU2002341898A2 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation

Publications (2)

Publication Number Publication Date
WO2003027640A2 true WO2003027640A2 (en) 2003-04-03
WO2003027640A3 true true WO2003027640A3 (en) 2003-07-31

Family

ID=26680615

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/031073 WO2003027640A3 (en) 2001-03-27 2002-09-27 Polymorphism detection and separation

Country Status (2)

Country Link
EP (1) EP1446502A4 (en)
WO (1) WO2003027640A3 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945845B2 (en) 2002-02-21 2015-02-03 Alere San Diego Inc. Recombinase polymerase amplification
US9340825B2 (en) 2002-02-21 2016-05-17 Alere San Diego, Inc. Compositions for recombinase polymerase amplification
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2336361A3 (en) * 2004-06-01 2011-07-06 ASM Scientific, Inc. Kit for recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
DE10353419B3 (en) * 2003-11-09 2005-06-30 Epigenomics Ag A method for the examination of cytosine methylations in DNA sequences by means of restriction enzymes hemimethylierungssensitiver
WO2007032984A3 (en) * 2005-09-12 2007-08-30 William D Figg Cyp1b1 genotype
WO2008035205A3 (en) 2006-05-04 2009-01-15 Niall Antony Armes Recombinase polymerase amplification
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
EP2694666B1 (en) 2011-04-07 2018-05-23 Alere San Diego, Inc. Monitoring recombinase polymerase amplification mixtures

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2116215C (en) * 1990-05-07 1998-12-22 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double d-loop formation
US5929043A (en) * 1995-01-31 1999-07-27 Pangene Corporation Recombinase mediated DNA therapies
US20020061530A1 (en) * 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
US20020132259A1 (en) * 2001-02-21 2002-09-19 Wagner Robert E. Mutation detection using MutS and RecA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1446502A4 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945845B2 (en) 2002-02-21 2015-02-03 Alere San Diego Inc. Recombinase polymerase amplification
US9309502B2 (en) 2002-02-21 2016-04-12 Alere San Diego Inc. Recombinase polymerase amplification
US9340825B2 (en) 2002-02-21 2016-05-17 Alere San Diego, Inc. Compositions for recombinase polymerase amplification
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates

Also Published As

Publication number Publication date Type
WO2003027640A2 (en) 2003-04-03 application
EP1446502A4 (en) 2005-02-09 application
EP1446502A2 (en) 2004-08-18 application

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