WO2003025561A1 - METHOD OF SIMULTANEOUSLY MEASURING Rb VALUE AND PLASMA PROTEIN-BINDING RATIO - Google Patents

METHOD OF SIMULTANEOUSLY MEASURING Rb VALUE AND PLASMA PROTEIN-BINDING RATIO Download PDF

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Publication number
WO2003025561A1
WO2003025561A1 PCT/JP2002/009503 JP0209503W WO03025561A1 WO 2003025561 A1 WO2003025561 A1 WO 2003025561A1 JP 0209503 W JP0209503 W JP 0209503W WO 03025561 A1 WO03025561 A1 WO 03025561A1
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Prior art keywords
value
ratio
formula
plasma
simultaneously measuring
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PCT/JP2002/009503
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French (fr)
Japanese (ja)
Inventor
Masatomi Watanabe
Motohiro Kato
Original Assignee
Chugai Seiyaku Kabushiki Kaisha
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Application filed by Chugai Seiyaku Kabushiki Kaisha filed Critical Chugai Seiyaku Kabushiki Kaisha
Publication of WO2003025561A1 publication Critical patent/WO2003025561A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method of simultaneously measuring the Rb value and the plasma protein-binding ratio of a test compound in two blood samples having different dilution ratios which comprises the step of calculating the Rb value of the test compound in the samples and the hematocrit values of the samples in accordance with the following formula (I): Rb=(aVh+(xb+1)Vp)/(Vh+Vp)/(xb+1) formula (I) [wherein a represents the distribution ratio to hemocytes; b represents the maximum binding concentration/dissociation constant (nPt/Kd); Vh represents the hemocyte volume; Vp represents the plasma volume; and x represents the dilution ratio of plasma;] determining a and b in the above formula by the non-linear minimum square method, and then calculating using the following formula (II): protein-binding ratio=100b/(1+b) formula (II).
PCT/JP2002/009503 2001-09-14 2002-09-17 METHOD OF SIMULTANEOUSLY MEASURING Rb VALUE AND PLASMA PROTEIN-BINDING RATIO WO2003025561A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001-279975 2001-09-14
JP2001279975 2001-09-14

Publications (1)

Publication Number Publication Date
WO2003025561A1 true WO2003025561A1 (en) 2003-03-27

Family

ID=19104062

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/009503 WO2003025561A1 (en) 2001-09-14 2002-09-17 METHOD OF SIMULTANEOUSLY MEASURING Rb VALUE AND PLASMA PROTEIN-BINDING RATIO

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WO (1) WO2003025561A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2664920A1 (en) 2012-05-18 2013-11-20 Debreceni Egyetem Dilution based inhibition assay
CN115856060A (en) * 2023-02-17 2023-03-28 昭衍（苏州）新药研究中心有限公司 Method for rapidly detecting binding rate of oligonucleotide drugs and human and experimental animal plasma proteins

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09236603A (en) * 1995-12-26 1997-09-09 Wako Pure Chem Ind Ltd Stabilization method
JPH09288105A (en) * 1996-04-19 1997-11-04 Eiken Chem Co Ltd Reagent for measuring biological component

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09236603A (en) * 1995-12-26 1997-09-09 Wako Pure Chem Ind Ltd Stabilization method
JPH09288105A (en) * 1996-04-19 1997-11-04 Eiken Chem Co Ltd Reagent for measuring biological component

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2664920A1 (en) 2012-05-18 2013-11-20 Debreceni Egyetem Dilution based inhibition assay
CN115856060A (en) * 2023-02-17 2023-03-28 昭衍（苏州）新药研究中心有限公司 Method for rapidly detecting binding rate of oligonucleotide drugs and human and experimental animal plasma proteins
CN115856060B (en) * 2023-02-17 2023-09-29 昭衍（苏州）新药研究中心有限公司 Method for rapidly detecting binding rate of oligonucleotide drug and human and experimental animal plasma protein

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