WO2003025218A3 - Methods and yeast strains for screening antifungal agents - Google Patents

Methods and yeast strains for screening antifungal agents Download PDF

Info

Publication number
WO2003025218A3
WO2003025218A3 PCT/BE2002/000146 BE0200146W WO03025218A3 WO 2003025218 A3 WO2003025218 A3 WO 2003025218A3 BE 0200146 W BE0200146 W BE 0200146W WO 03025218 A3 WO03025218 A3 WO 03025218A3
Authority
WO
WIPO (PCT)
Prior art keywords
gpr1
yeast
methods
protein
yeast strains
Prior art date
Application number
PCT/BE2002/000146
Other languages
French (fr)
Other versions
WO2003025218A2 (en
Inventor
Katleen Lemaire
Rop Larissa De
Dijck Patrick Van
Johan Thevelein
Original Assignee
Leuven K U Res & Dev
Katleen Lemaire
Rop Larissa De
Dijck Patrick Van
Johan Thevelein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0122698A external-priority patent/GB0122698D0/en
Priority claimed from GB0218220A external-priority patent/GB0218220D0/en
Application filed by Leuven K U Res & Dev, Katleen Lemaire, Rop Larissa De, Dijck Patrick Van, Johan Thevelein filed Critical Leuven K U Res & Dev
Priority to AU2002342447A priority Critical patent/AU2002342447A1/en
Publication of WO2003025218A2 publication Critical patent/WO2003025218A2/en
Publication of WO2003025218A3 publication Critical patent/WO2003025218A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Oncology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Botany (AREA)
  • Communicable Diseases (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)

Abstract

The Gpr1 G-protein coupled receptor protein is used as a target for antifungals against pathogenic fungi. The invention constitutes an assay for selecting compounds that modulate or decrease the sugar-receptor function of GPR1 in hyphae forming yeast. Hyphae forming yeast strains which are deleted for one allele of the GPR1 gene are used to screen for inhibitors of the Gpr1 protein. The invention also features strains of originally filamentous virulent yeast transformed to render said yeast to be nonfilamentous and avirulent to control fungal infectious diseases.
PCT/BE2002/000146 2001-09-21 2002-09-23 Methods and yeast strains for screening antifungal agents WO2003025218A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002342447A AU2002342447A1 (en) 2001-09-21 2002-09-23 Methods and yeast strains for screening antifungal agents

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0122698.4 2001-09-21
GB0122698A GB0122698D0 (en) 2001-09-21 2001-09-21 Antifungal drugs
GB0218220A GB0218220D0 (en) 2002-08-06 2002-08-06 Antifungal drugs
GB0218220.2 2002-08-06

Publications (2)

Publication Number Publication Date
WO2003025218A2 WO2003025218A2 (en) 2003-03-27
WO2003025218A3 true WO2003025218A3 (en) 2003-12-11

Family

ID=26246567

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BE2002/000146 WO2003025218A2 (en) 2001-09-21 2002-09-23 Methods and yeast strains for screening antifungal agents

Country Status (2)

Country Link
AU (1) AU2002342447A1 (en)
WO (1) WO2003025218A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005080588A2 (en) * 2004-02-19 2005-09-01 F2G Ltd 2031 oxidoreductase
WO2010010027A1 (en) * 2008-07-23 2010-01-28 Vib Vzw Antifungal compounds
US20130130305A1 (en) * 2010-05-17 2013-05-23 Reeta Prusty Rao Indentifying antifungal agents that inhibit iaa or a yap family

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999063094A2 (en) * 1998-06-01 1999-12-09 Mount Sinai School Of Medicine Of New York University Nucleotide and protein sequences of gpr1 and methods based thereon
WO2001016357A2 (en) * 1999-08-30 2001-03-08 K.U. Leuven Research & Development Novel target for antiparasitic agents and inhibitors thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999063094A2 (en) * 1998-06-01 1999-12-09 Mount Sinai School Of Medicine Of New York University Nucleotide and protein sequences of gpr1 and methods based thereon
WO2001016357A2 (en) * 1999-08-30 2001-03-08 K.U. Leuven Research & Development Novel target for antiparasitic agents and inhibitors thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KRAAKMAN LEON ET AL: "A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose.", MOLECULAR MICROBIOLOGY, vol. 32, no. 5, June 1999 (1999-06-01), pages 1002 - 1012, XP002246256, ISSN: 0950-382X *
VAN DIJCK PATRICK ET AL: "Characterization of a new set of mutants deficient in fermentation-induced loss of stress resistance for use in frozen dough applications.", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, vol. 55, no. 1-3, 10 April 2000 (2000-04-10), pages 187 - 192, XP002246257, ISSN: 0168-1605 *
YUN CHEOL-WON ET AL: "Gpr1p, a putative G-protein coupled receptor, regulates glucose-dependent cellular cAMP level in yeast Saccharomyces cerevisiae.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 252, no. 1, 9 November 1998 (1998-11-09), pages 29 - 33, XP002246255, ISSN: 0006-291X *

Also Published As

Publication number Publication date
WO2003025218A2 (en) 2003-03-27
AU2002342447A1 (en) 2003-04-01

Similar Documents

Publication Publication Date Title
Escudero et al. Effects on plant growth and root-knot nematode infection of an endophytic GFP transformant of the nematophagous fungus Pochonia chlamydosporia
Niimi et al. Overexpression of Candida albicans CDR1, CDR2, or MDR1 does not produce significant changes in echinocandin susceptibility
Niimi et al. Chemosensitization of fluconazole resistance in Saccharomyces cerevisiae and pathogenic fungi by a D-octapeptide derivative
WO2001049823A3 (en) Assays for detection of bacillus anthracis
Leroch et al. Living colors in the gray mold pathogen Botrytis cinerea: codon-optimized genes encoding green fluorescent protein and mCherry, which exhibit bright fluorescence
Stichternoth et al. Sch9 kinase integrates hypoxia and CO2 sensing to suppress hyphal morphogenesis in Candida albicans
BR0014282A (en) Vaccines
Shewmaker et al. Ure2p function is enhanced by its prion domain in Saccharomyces cerevisiae
WO2014086750A3 (en) Composition comprising a biological control agent and an insecticide
EP1291823A3 (en) Methods for determining intersections
Sakoh-Nakatogawa et al. Localization of Atg3 to autophagy-related membranes and its enhancement by the Atg8-family interacting motif to promote expansion of the membranes
Zhou et al. β1 tubulin rather than β2 tubulin is the preferred binding target for carbendazim in Fusarium graminearum
Burt et al. Isolation and partial characterization of Hsp90 from Candida albicans
WO2003025218A3 (en) Methods and yeast strains for screening antifungal agents
Zahradnik et al. A new immunoassay to quantify fungal antigens from the indoor mould Aspergillus versicolor
WO2003016568A3 (en) Yeast arrays, methods of making such arrays, and methods of analyzing such arrays
Rupp Proteomics on its way to study host–pathogen interaction in Candida albicans
WO1997038293A3 (en) Assays and reagents for identifying anti-fungal agents, and uses related thereto
WO2005123966A3 (en) Compositions and methods for diagnosis and treatment of orthopoxviruses
AU2002210982A1 (en) Method of screening antiplatelet
Buurman et al. Pyridines and pyrimidines mediating activity against an efflux-negative strain of Candida albicans through putative inhibition of lanosterol demethylase
Shaw et al. Ions as regulators of growth and development
DE69432422D1 (en) METHODS FOR DETECTING ANTI-ESTROGEN COMPOUNDS
WO2001061019A3 (en) A dominant selectable marker for gene transformation and disruption in yeasts
WO2004087070A3 (en) METHODS OF IDENTIFYING COMPOUNDS THAT TARGET tRNA SPLICING ENDONUCLEASE AND USES OF SAID COMPOUNDS AS ANTI-FUNGAL AGENTS

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BY BZ CA CH CN CO CR CU CZ DE DM DZ EC EE ES FI GB GD GE GH HR HU ID IL IN IS JP KE KG KP KR LC LK LR LS LT LU LV MA MD MG MN MW MX MZ NO NZ OM PH PL PT RU SD SE SG SI SK SL TJ TM TN TR TZ UA UG US UZ VN YU ZA ZM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ UG ZM ZW AM AZ BY KG KZ RU TJ TM AT BE BG CH CY CZ DK EE ES FI FR GB GR IE IT LU MC PT SE SK TR BF BJ CF CG CI GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP