WO2003020877A3 - Coloration et tri de genomes et de chromosomes au moyen de polyamides specifiques de sequences - Google Patents

Coloration et tri de genomes et de chromosomes au moyen de polyamides specifiques de sequences Download PDF

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Publication number
WO2003020877A3
WO2003020877A3 PCT/US2002/021841 US0221841W WO03020877A3 WO 2003020877 A3 WO2003020877 A3 WO 2003020877A3 US 0221841 W US0221841 W US 0221841W WO 03020877 A3 WO03020877 A3 WO 03020877A3
Authority
WO
WIPO (PCT)
Prior art keywords
sorting
chromosomes
staining
sequence
cells
Prior art date
Application number
PCT/US2002/021841
Other languages
English (en)
Other versions
WO2003020877A2 (fr
Inventor
Bradley Didion
James Bashkin
Scott Woodard
Original Assignee
Pharmacia Corp
Bradley Didion
James Bashkin
Scott Woodard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Corp, Bradley Didion, James Bashkin, Scott Woodard filed Critical Pharmacia Corp
Priority to AU2002320399A priority Critical patent/AU2002320399A1/en
Publication of WO2003020877A2 publication Critical patent/WO2003020877A2/fr
Publication of WO2003020877A3 publication Critical patent/WO2003020877A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne des ADN génomiques de cellules reproductrices, y compris le sperme et d'autres cellules importantes en matière de reproduction, qui sont colorés et éventuellement triés par ciblage de polyamides oligomériques susceptibles de fluorescence pour cibler des séquences d'ADN, puis par tri des cellules, celles, par exemple, qui sont activées par fluorescence ou par d'autres techniques de séparation cellulaire.
PCT/US2002/021841 2001-09-01 2002-08-15 Coloration et tri de genomes et de chromosomes au moyen de polyamides specifiques de sequences WO2003020877A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002320399A AU2002320399A1 (en) 2001-09-01 2002-08-15 Staining and sorting genomes and chromosomes using sequence-specific polyamides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31672901P 2001-09-01 2001-09-01
US60/316,729 2001-09-01

Publications (2)

Publication Number Publication Date
WO2003020877A2 WO2003020877A2 (fr) 2003-03-13
WO2003020877A3 true WO2003020877A3 (fr) 2003-12-11

Family

ID=23230389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021841 WO2003020877A2 (fr) 2001-09-01 2002-08-15 Coloration et tri de genomes et de chromosomes au moyen de polyamides specifiques de sequences

Country Status (2)

Country Link
AU (1) AU2002320399A1 (fr)
WO (1) WO2003020877A2 (fr)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
EP2258172B1 (fr) 2000-05-09 2017-04-19 Xy, Llc Cytométre de flux pour différencier des populations de spermatozoïdes portant un chromosome X et un chromosome Y
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
CA2822983C (fr) 2000-11-29 2017-05-09 Xy, Llc Systeme de dissociation de spermatozoides congeles-decongeles porteurs d'un chromosome x de ceux porteurs d'un chromosome y
JP4595067B2 (ja) 2002-08-01 2010-12-08 エックスワイ，エルエルシー 低圧精子細胞分離システム
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
CN100570360C (zh) 2002-08-15 2009-12-16 Xy公司 一种流式细胞仪及流式细胞计数方法
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305171T3 (da) 2003-03-28 2022-03-21 Inguran Llc Apparat og fremgangsmåder til tilvejebringelse af kønssorteret dyresæd
ES2541121T3 (es) 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
US8017599B2 (en) 2003-10-07 2011-09-13 University Of Western Sydney Sequence selective pyrrole and imidazole polyamide metallocomplexes
US7892725B2 (en) 2004-03-29 2011-02-22 Inguran, Llc Process for storing a sperm dispersion
AU2005266930B2 (en) 2004-07-22 2010-09-16 Inguran, Llc Process for enriching a population of sperm cells
US7709244B2 (en) 2007-01-08 2010-05-04 The Invention Science Fund I, Llc Systems for genome selection
US7648823B2 (en) 2007-01-08 2010-01-19 Searete Llc Systems for genome selection
US7947457B2 (en) 2007-01-08 2011-05-24 The Invention Science Fund I, Llc Systems for genome selection
US7718418B2 (en) 2007-01-08 2010-05-18 The Invention Science Fund I, Llc Systems for genome selection
US8012740B2 (en) 2007-01-08 2011-09-06 The Invention Science Fund I, Llc Systems for genome selection
US8521440B2 (en) 2007-01-08 2013-08-27 The Invention Science Fund I, Llc Systems for genome selection
US8003371B2 (en) 2007-01-08 2011-08-23 The Invention Science Fund I, Llc Systems for genome selection
US7709245B2 (en) 2007-01-08 2010-05-04 The Invention Science Fund I, Llc Systems for genome selection
US7947455B2 (en) 2007-01-08 2011-05-24 The Invention Science Fund I, Llc System for genome selection
US7985578B2 (en) 2007-01-08 2011-07-26 The Invention Science Fund I, Llc Systems for genome selection
US7713728B2 (en) 2007-01-08 2010-05-11 The Invention Sciencefund I, Llc Systems for genome selection
AU2008338530A1 (en) * 2007-12-14 2009-06-25 Minitube Of America, Inc. Gender-specific separation of sperm cells and embryos
WO2014055112A1 (fr) 2012-10-05 2014-04-10 Inguran, Llc Procédés à haute efficacité de tri de spermes par sexe

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BITO ET AL.: "Differences in the DNA-stainability of spermatozoa from fertile and suspected infertile men", INTERNATIONAL JOURNAL OF ANDROLOGY, vol. 22, 1999, pages 275 - 281, XP002967851 *

Also Published As

Publication number Publication date
WO2003020877A2 (fr) 2003-03-13
AU2002320399A1 (en) 2003-03-18

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