WO2003014382B1 - Device for analyzing nucleic acid - Google Patents
Device for analyzing nucleic acidInfo
- Publication number
- WO2003014382B1 WO2003014382B1 PCT/AT2002/000239 AT0200239W WO03014382B1 WO 2003014382 B1 WO2003014382 B1 WO 2003014382B1 AT 0200239 W AT0200239 W AT 0200239W WO 03014382 B1 WO03014382 B1 WO 03014382B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- oligonucleotide primer
- dna sequence
- primer consisting
- nucleic acid
- amplification
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims 8
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 20
- 108091034117 Oligonucleotide Proteins 0.000 claims 16
- 230000003321 amplification Effects 0.000 claims 7
- 238000000034 method Methods 0.000 claims 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims 7
- 239000002773 nucleotide Substances 0.000 claims 7
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 230000001580 bacterial effect Effects 0.000 claims 4
- 241000894007 species Species 0.000 claims 4
- 108020004465 16S ribosomal RNA Proteins 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 241000606208 Campylobacter gracilis Species 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 238000007834 ligase chain reaction Methods 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 239000013641 positive control Substances 0.000 claims 2
- 238000012340 reverse transcriptase PCR Methods 0.000 claims 2
- 241000186066 Actinomyces odontolyticus Species 0.000 claims 1
- 241000186044 Actinomyces viscosus Species 0.000 claims 1
- 241000606749 Aggregatibacter actinomycetemcomitans Species 0.000 claims 1
- 108091093088 Amplicon Proteins 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 241000589873 Campylobacter concisus Species 0.000 claims 1
- 241000589996 Campylobacter rectus Species 0.000 claims 1
- 241000190888 Capnocytophaga gingivalis Species 0.000 claims 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims 1
- 241000588878 Eikenella corrodens Species 0.000 claims 1
- 241000605986 Fusobacterium nucleatum Species 0.000 claims 1
- 241001464887 Parvimonas micra Species 0.000 claims 1
- 241000605862 Porphyromonas gingivalis Species 0.000 claims 1
- 241001135221 Prevotella intermedia Species 0.000 claims 1
- 241001135225 Prevotella nigrescens Species 0.000 claims 1
- 241001291896 Streptococcus constellatus Species 0.000 claims 1
- 241000194026 Streptococcus gordonii Species 0.000 claims 1
- 241001134658 Streptococcus mitis Species 0.000 claims 1
- 241001135235 Tannerella forsythia Species 0.000 claims 1
- 241000589892 Treponema denticola Species 0.000 claims 1
- 241001148135 Veillonella parvula Species 0.000 claims 1
- 241001494431 [Eubacterium] nodatum Species 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 235000013351 cheese Nutrition 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 208000028169 periodontal disease Diseases 0.000 claims 1
- 201000001245 periodontitis Diseases 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 230000002285 radioactive effect Effects 0.000 claims 1
- 239000013598 vector Substances 0.000 claims 1
- 239000012491 analyte Substances 0.000 abstract 2
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00693—Calibration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Quality & Reliability (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a device for analyzing at least one analyte from a sample. The inventive device comprises a support (1) with a surface (2) on which at least one predefined analytical zone (3) and at least one predefined control zone (4) are arranged. At least one first binding partner which specifically binds to at least one analyte is immobilized in the predefined analytical zone (3). At least one further binding partner is disposed in the at least one control zone (4) and allows at least one control for determining the quality of analysis.
Claims
40 40
GEÄNDERTE ANSPRÜCHECHANGED CLAIMS
[beim Internationalen Büro am 12. August 2003 (12.08.03) eingegangen, ursprüngliche Ansprüche 22, 23, 28 geändert][received by the International Bureau on 12 August 2003 (12.08.03), original claims 22, 23, 28 amended]
21. Vorrichtung nach Anspruch 20, dadurch gekennzeichnet, daß das mit Parodontitis, insbesondere Periodontitis, assoziierte Bakterium aus einer Gruppe umfassend Actinobacillus actinomycetemcomitans, Actinomyces odontolyticus, Actinomyces viscosus, Bacteroides forsythus, Campylobacter concisus, Campylobacter gracilis (Bacteroides gracilis), Campylo- bacter rectus, Capnocytophaga gingivalis, Eikenella corrodens, Eubacterium nodatum, Fuso- bacterium nucleatum, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella in- termedia, Prevotella nigrescens, Streptococcus constellatus, Streptococcus gordonii, Streptococcus mitis, Treponema denticola oder Veillonella parvula ausgewählt ist.21. The device according to claim 20, characterized in that associated with periodontal disease, in particular periodontitis, bacterium from a group comprising Actinobacillus actinomycetemcomitans, Actinomyces odontolyticus, Actinomyces viscosus, Bacteroides forsythus, Campylobacter concisus, Campylobacter gracilis (Bacteroides gracilis), Campylobacter rectus , Capnocytophaga gingivalis, Eikenella corrodens, Eubacterium nodatum, Fusobacterium nucleatum, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Streptococcus constellatus, Streptococcus gordonii, Streptococcus mitis, Treponema denticola or Veillonella parvula.
22. Vorrichtung nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, daß die Sequenz der zumindest einen Positivkontrolle aus einer Gruppe von Sequenzen des Gens für eine 16S-rRNA der Bakterienarten gemäß Anspruch 21 oder Teilsequenzen daraus ausgewählt ist, die mit einer Nukleinsäuresequenz hybridisierbar ist, die mittels zumindest einem Oligonukleotidprimer bestehend aus der DNA-Sequenz 5 '-AACAGGATTAGATACCCTGGTAGTCC - 3 ' und/oder einem Oligonukleotidprimer bestehend aus der DNA-Sequenz 5'- CAYYTCACGACACGAGCTGACGACA - 3' und/oder einem Oligonukleotidprimer bestehend aus zumindest 15 aufeinanderfolgenden Nukleotiden der DNA-Sequenz 5'- GGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACG - 3' und/oder einem Oligonukleotidprimer bestehend aus zumindest 15 aufeinanderfolgenden Nukleotiden der DNA-Sequenz22. Device according to one of the preceding claims, characterized in that the sequence of the at least one positive control from a group of sequences of the gene for a 16S rRNA of the bacterial species according to claim 21 or partial sequences thereof is selected, which is hybridizable with a nucleic acid sequence, the by means of at least one oligonucleotide primer consisting of the DNA sequence 5 '-AACAGGATTAGATACCCTGGTAGTCC - 3' and / or an oligonucleotide primer consisting of the DNA sequence 5'-CAYYTCACGACACGAGCTGACGACA - 3 'and / or an oligonucleotide primer consisting of at least 15 consecutive nucleotides of the DNA sequence 5'-GGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACG - 3 'and / or an oligonucleotide primer consisting of at least 15 contiguous nucleotides of the DNA sequence
5'- CCCAACAYYTCACGACACGAGCTGACGACAGCCAT - 3' amplifizierbar ist.5'-CCCAACAYYTCACGACACGAGCTGACGACAGCCAT - 3 'is amplifiable.
23. Vorrichtung nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, daß die Zielnukleinsäuresequenz aus Sequenzen des Gens für eine 16S-rRNA der Bakterien- arten gemäß Anspruch 21 oder Teilsequenzen daraus ausgewählt ist, die mit zumindest einem23. Device according to one of the preceding claims, characterized in that the target nucleic acid sequence is selected from sequences of the gene for a 16S rRNA of the bacterial species according to claim 21 or subsequences thereof, which with at least one
Oligonukleotidprimer bestehend aus der DNA-SequenzOligonucleotide primer consisting of the DNA sequence
5'-AACAGGATTAGATACCCTGGTAGTCC - 3' und oder einem Oligonukleotidprimer bestehend aus der DNA-Sequenz 5'- CAYYTCACGACACGAGCTGACGACA - 3' und/oder einem Oligonukleotidprimer bestehend aus zumindest 15 aufeinanderfolgenden Nukleotiden der DNA-Sequenz 5'- GGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACG - 3' und/oder einem Oligonukleotidprimer bestehend aus zumindest 15 aufeinanderfolgenden Nukleotiden der DNA-Sequenz 5'- CCCAACAYYTCACGACACGAGCTGACGACAGCCAT - 3' amplifizierbar ist.5'-AACAGGATTAGATACCCTGGTAGTCC - 3 'and / or an oligonucleotide primer consisting of the DNA sequence 5'-CAYYTCACGACACGAGCTGACGACA - 3' and / or an oligonucleotide primer consisting of at least 15 consecutive nucleotides of the DNA sequence 5'GGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACG - 3 'and / or Oligonucleotide primer consisting of at least 15 consecutive nucleotides of the DNA sequence 5'-CCCAACAYYTCACGACACGAGCTGACGACAGCCAT - 3 'is amplified.
24. Verfahren zur Amplifikation von zumindest einer Zielnukleinsäuresequenz, insbe -
4124. A method for amplifying at least one target nucleic acid sequence, in particular 41
sondere mittels Polymerase-Kettenreaktion (PCR), Ligase-Kettenreaktion (LCR), Transkrip¬ tion vermittelte Amplifikation (TMA), Reverse Transkriptase PCR (RT-PCR), Q-beta Repli-
sondere by polymerase chain reaction (PCR), ligase chain reaction (LCR), Transkrip ¬ tion mediated amplification (TMA), reverse transcriptase PCR (RT-PCR), Q-beta replicants
4242
käse Amplifizierung, Einzelstrang-Displacement Amplifizierung oder Amplicon- Vektoren, dadurch gekennzeichnet, daß zur Amplifikation zumindest ein Oligonukleotidprimer bestehend aus der DNA-Sequenz 5'- AACAGGATTAGATACCCTGGTAGTCC - 3' und/oder ein Oligonukleotidprimer bestehend aus der DNA- SequenzCheese Amplification, single-stranded displacement amplification or amplicon vectors, characterized in that for amplification at least one oligonucleotide primer consisting of the DNA sequence 5'AACAGGATTAGATACCCTGGTAGTCC - 3 'and / or an oligonucleotide primer consisting of the DNA sequence
5'- CAYYTCACGACACGAGCTGACGACA - 3' und/oder ein Oligonukleotidprimer bestehend aus zumindest 15 aufeinanderfolgenden Nukleotiden der DNA-Sequenz 5'- GGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACG - 3' und/oder ein Oligonukleotidprimer bestehend aus zumindest 15 aufeinanderfolgenden Nukleotiden der DNA- Sequenz 5'- CCCAACAYYTCACGACACGAGCTGACGACAGCCAT - 3' verwendet wird.5'-CAYYTCACGACACGAGCTGACGACA-3 'and / or an oligonucleotide primer consisting of at least 15 contiguous nucleotides of the DNA sequence 5'-GGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACG-3' and / or an oligonucleotide primer consisting of at least 15 consecutive nucleotides of the DNA sequence 5'-CCCAACAYYTCACGACACGAGCTGACGACAGCCAT-3 ' is used.
25. Verfahren nach Anspruch 25, dadurch gekennzeichnet, daß mehrere Zielnukleinsäu- resequenzen für verschiedene Bakterienarten gleichzeitig amplifiziert werden.25. The method according to claim 25, characterized in that several Zielnukleinsäu- resequenzen for different bacterial species are amplified simultaneously.
26. Verfahren nach Anspruch 24 oder 25, dadurch gekennzeichnet, daß eine Zielnuklein- säuresequenz des Gens für eine 16S-rRNA der Bakterienarten oder Teilsequenzen daraus amplifiziert werden.26. The method according to claim 24 or 25, characterized in that a target nucleic acid sequence of the gene for a 16S rRNA of the bacterial species or partial sequences thereof are amplified.
27. Verfahren nach einem der Ansprüche 24 bis 26, dadurch gekennzeichnet, daß für die Amplifikation Digoxigenin-, Biotin-, radioaktive Markierungen, wie z. B. 33P, Floureszenz- farbstoff-markierte Oligonukleotidprimer bzw. Nukleotide und/oder Markierungen, die mit optischen, elektrischen, elektrochemischen, magnetischen, photochemischen, photoelektrischen oder enzymatischen Detektionsverfahren nachgewiesen werden, verwendet werden.27. The method according to any one of claims 24 to 26, characterized in that for the amplification digoxigenin, biotin, radioactive labels, such as. B. 33 P, fluorescent dye-labeled oligonucleotide primer or nucleotides and / or labels, which are detected by optical, electrical, electrochemical, magnetic, photochemical, photoelectric or enzymatic detection methods can be used.
28. Verfahren zur Identifikation von zumindest einer Zielnukleinsäuresequenz umfas- send die Schritte Probenaufbereitung, Amplifikation, Markierung, Hybridisierung komplementärer Sequenzen an ein Oligonukleotid und Dete tion des Hybridisierungssignals, dadurch gekennzeichnet, daß Amplifikate erzeugt werden, die mittels einer Amplifikation mit einem Verfahren nach einem der Ansprüche 24 bis 27 hergestellt werden.28. A method for the identification of at least one target nucleic acid sequence comprises the steps of sample preparation, amplification, labeling, hybridization of complementary sequences to an oligonucleotide and dete tion of the hybridization signal, characterized in that amplificates are generated, which by means of an amplification with a method according to one of Claims 24 to 27 are produced.
29. Verfahren nach Anspruch 28, dadurch gekennzeichnet, daß die Amplifikate an zumindest ein Oligonukleotid für zumindest eine Positivkontrolle und zumindest eine Zielnukleinsäuresequenz auf demselben Träger binden.29. The method according to claim 28, characterized in that the amplificates bind to at least one oligonucleotide for at least one positive control and at least one Zielnukleinsäuresequenz on the same carrier.
30. Oligonukleotidprimer mit einer Nukleinsäuresequenz, dadurch gekennzeichnet, daß die Nukleinsäuresequenz eine DNA-Sequenz aus einer Gruppe bestehend aus den Se-
30. Oligonucleotide primer with a nucleic acid sequence, characterized in that the nucleic acid sequence is a DNA sequence selected from a group consisting of
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02794485A EP1415003A2 (en) | 2001-08-09 | 2002-08-08 | Device for analyzing nucleic acid |
| AU2002332940A AU2002332940A1 (en) | 2001-08-09 | 2002-08-08 | Device for analyzing nucleic acid |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0124701A AT411174B (en) | 2001-08-09 | 2001-08-09 | METHOD AND CHIP FOR ANALYZING NUCLEIC ACIDS |
| ATA1247/2001 | 2001-08-09 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| WO2003014382A2 WO2003014382A2 (en) | 2003-02-20 |
| WO2003014382A3 WO2003014382A3 (en) | 2003-10-23 |
| WO2003014382B1 true WO2003014382B1 (en) | 2004-02-12 |
| WO2003014382A8 WO2003014382A8 (en) | 2004-04-08 |
Family
ID=3687796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/AT2002/000239 WO2003014382A2 (en) | 2001-08-09 | 2002-08-08 | Device for analyzing nucleic acid |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1415003A2 (en) |
| AT (1) | AT411174B (en) |
| AU (1) | AU2002332940A1 (en) |
| WO (1) | WO2003014382A2 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20040121309A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues |
| US7217510B2 (en) | 2001-06-26 | 2007-05-15 | Isis Pharmaceuticals, Inc. | Methods for providing bacterial bioagent characterizing information |
| US8073627B2 (en) | 2001-06-26 | 2011-12-06 | Ibis Biosciences, Inc. | System for indentification of pathogens |
| JP2006516193A (en) | 2002-12-06 | 2006-06-29 | アイシス・ファーマシューティカルス・インコーポレーテッド | Rapid identification of pathogens in humans and animals |
| US8057993B2 (en) | 2003-04-26 | 2011-11-15 | Ibis Biosciences, Inc. | Methods for identification of coronaviruses |
| US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US8097416B2 (en) | 2003-09-11 | 2012-01-17 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| US8546082B2 (en) | 2003-09-11 | 2013-10-01 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| US20120122099A1 (en) | 2003-09-11 | 2012-05-17 | Rangarajan Sampath | Compositions for use in identification of bacteria |
| US8163895B2 (en) | 2003-12-05 | 2012-04-24 | Ibis Biosciences, Inc. | Compositions for use in identification of orthopoxviruses |
| DE10357677A1 (en) | 2003-12-10 | 2005-07-14 | Greiner Bio-One Gmbh | Primers and probes for detecting genital HPV genotypes |
| US7666592B2 (en) | 2004-02-18 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
| ES2641832T3 (en) | 2004-05-24 | 2017-11-14 | Ibis Biosciences, Inc. | Mass spectrometry with selective ion filtration by setting digital thresholds |
| US20050266411A1 (en) | 2004-05-25 | 2005-12-01 | Hofstadler Steven A | Methods for rapid forensic analysis of mitochondrial DNA |
| US7811753B2 (en) | 2004-07-14 | 2010-10-12 | Ibis Biosciences, Inc. | Methods for repairing degraded DNA |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989006704A1 (en) * | 1988-01-11 | 1989-07-27 | Microprobe Corporation | Oligonucleotide probes for detection of periodontal pathogens |
| CA2150986C (en) * | 1994-06-17 | 1999-11-02 | Mary Kathryn Meyer | Oligonucleotide primers and probes for detection of bacteria |
| US6054278A (en) * | 1997-05-05 | 2000-04-25 | The Perkin-Elmer Corporation | Ribosomal RNA gene polymorphism based microorganism identification |
| DE19822108A1 (en) * | 1998-05-12 | 2000-02-03 | Schering Ag | Method for the detection of microorganisms in products, in particular in medicines and cosmetics |
| DE19944168A1 (en) * | 1999-09-15 | 2000-03-23 | Ulrich Bohr | Helicobacter and Wolinella species-specific detection and identification using new oligonucleotide primers directed to 16S rRNA consensus sequences |
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2002
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- 2002-08-08 EP EP02794485A patent/EP1415003A2/en not_active Withdrawn
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| AU2002332940A1 (en) | 2003-02-24 |
| AT411174B (en) | 2003-10-27 |
| WO2003014382A8 (en) | 2004-04-08 |
| WO2003014382A3 (en) | 2003-10-23 |
| WO2003014382A2 (en) | 2003-02-20 |
| EP1415003A2 (en) | 2004-05-06 |
| ATA12472001A (en) | 2003-03-15 |
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