WO2003012129A3 - Shikimate kinase from s. aureus for drug discovery - Google Patents

Shikimate kinase from s. aureus for drug discovery Download PDF

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Publication number
WO2003012129A3
WO2003012129A3 PCT/GB2002/003424 GB0203424W WO03012129A3 WO 2003012129 A3 WO2003012129 A3 WO 2003012129A3 GB 0203424 W GB0203424 W GB 0203424W WO 03012129 A3 WO03012129 A3 WO 03012129A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
shikimate kinase
polypeptide
kinase activity
aureus
Prior art date
Application number
PCT/GB2002/003424
Other languages
French (fr)
Other versions
WO2003012129A2 (en
Inventor
Ian George Charles
Original Assignee
Arrow Therapeutics Ltd
Ian George Charles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arrow Therapeutics Ltd, Ian George Charles filed Critical Arrow Therapeutics Ltd
Priority to AU2002319475A priority Critical patent/AU2002319475A1/en
Publication of WO2003012129A2 publication Critical patent/WO2003012129A2/en
Publication of WO2003012129A3 publication Critical patent/WO2003012129A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01071—Shikimate kinase (2.7.1.71)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Abstract

A method for identification of an inhibitor of shikimate kinase activity and/or expression, which method comprises determining whether a test substance can inhibit: (I) activity of a polypeptide which comprises: (i) the amino acid sequence of SEQ ID NO:2; (ii) a variant thereof which is capable of shikimate kinase activity; or (iii) a fragment of (i) or (ii); and/or (II) transcription and/or translation of a polynucleotide encoding a polypeptide of (I); and/or (III) transcription and/or translation of a polynucleotide encoding a polypeptide capable of shikimate kinase activity and which polynucleotide comprises: (a) the nucleic acid sequence of SEQ ID NO: 1 and/or a sequence complementary thereto; (b) a sequence which hybridises under stringent conditions to a sequence as defined in (a); (c) a sequence that is degenerate as a result of the genetic code to a sequence as defined in (a) or (b); or (d) a sequence having at least 60 % identity to a sequence as defined in (a), (b) or (c). Inhibitors identified by such a method are useful in therapy, in particular in the treatment of microbial infections.
PCT/GB2002/003424 2001-07-25 2002-07-25 Shikimate kinase from s. aureus for drug discovery WO2003012129A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002319475A AU2002319475A1 (en) 2001-07-25 2002-07-25 Shikimate kinase from s. aureus for drug discovery

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0118136.1 2001-07-25
GBGB0118136.1A GB0118136D0 (en) 2001-07-25 2001-07-25 Target for drug discovery

Publications (2)

Publication Number Publication Date
WO2003012129A2 WO2003012129A2 (en) 2003-02-13
WO2003012129A3 true WO2003012129A3 (en) 2003-05-08

Family

ID=9919163

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/003424 WO2003012129A2 (en) 2001-07-25 2002-07-25 Shikimate kinase from s. aureus for drug discovery

Country Status (3)

Country Link
AU (1) AU2002319475A1 (en)
GB (1) GB0118136D0 (en)
WO (1) WO2003012129A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10330234A1 (en) * 2003-07-04 2005-01-20 Bayer Cropscience Ag A method for identifying fungicidally active compounds based on fungi mevalonate kinases
CN101200483B (en) * 2007-05-11 2011-01-12 中国人民解放军第四军医大学 Antisense nucleic acid of resistant and tolerant dimethoxyphenecillin staphylococcus aureus drug resistant gene

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
10 June 1999 (1999-06-10), pages 1 - 3, XP002229664, Retrieved from the Internet <URL:www.biochem.arizona.edu/classes/bioc462/462bH2002/462bHonorsProjects/462bHonors1999/oks/Enzym.htm> [retrieved on 20030203] *
ACTA CRYSTALLOGRAPHICA SECTION D BIOLOGICAL CRYSTALLOGRAPHY, vol. 53, no. 5, 1997, pages 612 - 614, ISSN: 0907-4449 *
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 55, no. 3, 1991, pages 769 - 774, ISSN: 0002-1369 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1979, BOWEN J R ET AL: "IN-VIVO ACTIVITY PURIFICATION CULTIVAR SORDAN 70A AND CHARACTERIZATION OF SHIKIMATE KINASE EC-2.7.1.71 FROM SORGHUM-BICOLOR", XP002229666, Database accession no. PREV198069026108 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1979, KOSHIBA T: "ALI CYCLIC ACID METABOLISM IN PLANTS PART 12 PARTIAL PURIFICATION AND SOME PROPERTIES OF SHIKIMATE KINASE FROM PHASEOLUS-MUNGO SEEDLINGS", XP002229667, Database accession no. PREV197968063334 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1991, TAGUCHI H ET AL: "ENZYMATIC ALTERATION IN THE SHIKIMATE PATHWAY DURING DERIVATION OF MENAQUINONE-4-PRODUCING MUTANTS OF FLAVOBACTERIUM-SP 238-7", XP002229668, Database accession no. PREV199192045216 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, KRELL TINO ET AL: "Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi.", XP002232036, Database accession no. PREV199799798766 *
DATABASE EBI [online] Database accession no. Q99TV6 *
DATABASE EBI [online] XP002229669, Database accession no. IPR000623 *
KURODA M ET AL: "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.", LANCET. ENGLAND 21 APR 2001, vol. 357, no. 9264, 21 April 2001 (2001-04-21), pages 1225 - 1240, XP002229662, ISSN: 0140-6736 *
MURRAY ROBERT W ET AL: "Staphylococcus aureus cell extract transcription-translation assay: Firefly luciferase reporter system for evaluating protein translation inhibitors.", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 45, no. 6, June 2001 (2001-06-01), pages 1900 - 1904, XP002229663, ISSN: 0066-4804 *
pages 3 - 14, XP002229665, Retrieved from the Internet <URL:medical.faculty.ncl.ac.uk/biomed/sbg/Publications/HomePages/ARH> [retrieved on 20030203] *
PLANT AND CELL PHYSIOLOGY, vol. 20, no. 4, 1979, EN, pages 803 - 810, ISSN: 0032-0781 *
PLANT PHYSIOLOGY (BETHESDA), vol. 64, no. 3, 1979, pages 382 - 386, ISSN: 0032-0889 *

Also Published As

Publication number Publication date
AU2002319475A1 (en) 2003-02-17
WO2003012129A2 (en) 2003-02-13
GB0118136D0 (en) 2001-09-19

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