WO2003008631A3 - Tagging of microorganisms - Google Patents

Tagging of microorganisms Download PDF

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Publication number
WO2003008631A3
WO2003008631A3 PCT/GB2002/003343 GB0203343W WO03008631A3 WO 2003008631 A3 WO2003008631 A3 WO 2003008631A3 GB 0203343 W GB0203343 W GB 0203343W WO 03008631 A3 WO03008631 A3 WO 03008631A3
Authority
WO
WIPO (PCT)
Prior art keywords
microorganism
labelled
label
microorganisms
labelling
Prior art date
Application number
PCT/GB2002/003343
Other languages
French (fr)
Other versions
WO2003008631A2 (en
Inventor
Richard Vipond
Nigel Peter Minton
Desmond Purdy
Original Assignee
Microbiological Res Authority
Richard Vipond
Nigel Peter Minton
Desmond Purdy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0117787A external-priority patent/GB0117787D0/en
Priority claimed from GB0207605A external-priority patent/GB0207605D0/en
Application filed by Microbiological Res Authority, Richard Vipond, Nigel Peter Minton, Desmond Purdy filed Critical Microbiological Res Authority
Priority to AU2002317972A priority Critical patent/AU2002317972A1/en
Publication of WO2003008631A2 publication Critical patent/WO2003008631A2/en
Publication of WO2003008631A3 publication Critical patent/WO2003008631A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of labelling a microorganism comprises introducing a label into a first microorganism, wherein the phenotype of the labelled first microorganism is the same as that of a second microorganism is of the same strain as the first but is not labelled and, separately, mutating the first microorganism. In one method, the label is introduced into a phage integration site in the genome of the microorganism. In another the label is in an IStron. Gene identification and assays for gene function are carried out using microorganisms labelled in this way, and the invention also provides vectors for carrying out the labelling.
PCT/GB2002/003343 2001-07-20 2002-07-22 Tagging of microorganisms WO2003008631A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002317972A AU2002317972A1 (en) 2001-07-20 2002-07-22 Tagging of microorganisms

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0117787A GB0117787D0 (en) 2001-07-20 2001-07-20 Tagging of microorganisms
GB0117787.2 2001-07-20
GB0125102A GB0125102D0 (en) 2001-07-20 2001-10-18 Tagging of microorganisms
GB0125102.4 2001-10-18
GB0207605A GB0207605D0 (en) 2001-07-20 2002-04-02 Tagging of microorganisms
GB0207605.7 2002-04-02

Publications (2)

Publication Number Publication Date
WO2003008631A2 WO2003008631A2 (en) 2003-01-30
WO2003008631A3 true WO2003008631A3 (en) 2003-08-14

Family

ID=27256228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/003343 WO2003008631A2 (en) 2001-07-20 2002-07-22 Tagging of microorganisms

Country Status (1)

Country Link
WO (1) WO2003008631A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2373586T3 (en) 2006-05-19 2012-02-06 Danisco A/S MARKED MICROORGANISMS AND METHODS TO MARK.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996017951A2 (en) * 1994-12-09 1996-06-13 Rpms Technology Limited Identification of genes responsible for in vivo survival of microorganisms
WO1999007861A1 (en) * 1997-08-06 1999-02-18 Laboratório Medinfar-Produtos Farmacêuticos, Lda. DNA INTEGRATION INTO 'MYCOBACTERIUM spp.' GENOME BY TRANS-COMPLEMENTATION USING A SITE-SPECIFIC INTEGRATION SYSTEM
US6025190A (en) * 1992-03-13 2000-02-15 Institut National De La Recherche Agronomique Temperature-sensitive plasmid
WO2001002555A1 (en) * 1999-07-06 2001-01-11 Institut Pasteur Method of making and identifying attenuated microorganisms, compositions utilizing the sequences responsible for attenuation, and preparations containing attenuated microorganisms

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025190A (en) * 1992-03-13 2000-02-15 Institut National De La Recherche Agronomique Temperature-sensitive plasmid
WO1996017951A2 (en) * 1994-12-09 1996-06-13 Rpms Technology Limited Identification of genes responsible for in vivo survival of microorganisms
WO1999007861A1 (en) * 1997-08-06 1999-02-18 Laboratório Medinfar-Produtos Farmacêuticos, Lda. DNA INTEGRATION INTO 'MYCOBACTERIUM spp.' GENOME BY TRANS-COMPLEMENTATION USING A SITE-SPECIFIC INTEGRATION SYSTEM
WO2001002555A1 (en) * 1999-07-06 2001-01-11 Institut Pasteur Method of making and identifying attenuated microorganisms, compositions utilizing the sequences responsible for attenuation, and preparations containing attenuated microorganisms

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BRAUN VEIT ET AL: "A chimeric ribozyme in Clostridium difficile combines features of group I introns and insertion elements.", MOLECULAR MICROBIOLOGY, vol. 36, no. 6, June 2000 (2000-06-01), pages 1447 - 1459, XP002231557, ISSN: 0950-382X *
GROTH A C ET AL: "A phage integrase directs efficient site-specific integration in human cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 97, no. 11, 23 May 2000 (2000-05-23), pages 5995 - 6000, XP002221880, ISSN: 0027-8424 *
MACHOWSKI E E ET AL: "Construction and application of mycobacterial reporter transposons", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 253, no. 1, 25 July 2000 (2000-07-25), pages 67 - 75, XP004229159, ISSN: 0378-1119 *
PASCOPELLA L ET AL: "USE OF IN VIVO COMPLEMENTATION IN MYCOBACTERIUM TUBERCULOSIS TO IDENTIFY A GENOMIC FRAGMENT ASSOCIATED WITH VIRULENCE", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, US, vol. 62, no. 4, 1 April 1994 (1994-04-01), pages 1313 - 1319, XP002900090, ISSN: 0019-9567 *
SPRINGER B ET AL: "INSTABILITY AND SITE-SPECIFIC EXCISION OF INTEGRADTION-PROFICIENT MYCOBACTERIOPHAGE L5 PLASMIDS: DEVELOPMENT OF STABLY MAINTAINED INTEGRATIVE VECTORS", INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, URBAN UND FISCHER, DE, vol. 290, March 2001 (2001-03-01), pages 669 - 675, XP001069857, ISSN: 1438-4221 *
THYAGARAJAN BHASKAR ET AL: "Site-specific genomic integration in mammalian cells mediated by phage phiC31 integrase.", MOLECULAR AND CELLULAR BIOLOGY, vol. 21, no. 12, June 2001 (2001-06-01), pages 3926 - 3934, XP002231556, ISSN: 0270-7306 *

Also Published As

Publication number Publication date
WO2003008631A2 (en) 2003-01-30

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