WO2002103022A3 - Transgenic cells expressing clucosyltransferases which glucosylate parabenzoic acid - Google Patents

Transgenic cells expressing clucosyltransferases which glucosylate parabenzoic acid Download PDF

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Publication number
WO2002103022A3
WO2002103022A3 PCT/GB2002/002610 GB0202610W WO02103022A3 WO 2002103022 A3 WO2002103022 A3 WO 2002103022A3 GB 0202610 W GB0202610 W GB 0202610W WO 02103022 A3 WO02103022 A3 WO 02103022A3
Authority
WO
WIPO (PCT)
Prior art keywords
clucosyltransferases
glucosylate
transgenic cells
cells expressing
parabenzoic acid
Prior art date
Application number
PCT/GB2002/002610
Other languages
French (fr)
Other versions
WO2002103022A2 (en
Inventor
Eng Kiat Lim
Dianna Joy Bowles
Original Assignee
Univ York
Eng Kiat Lim
Dianna Joy Bowles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ York, Eng Kiat Lim, Dianna Joy Bowles filed Critical Univ York
Priority to AU2002345150A priority Critical patent/AU2002345150A1/en
Publication of WO2002103022A2 publication Critical patent/WO2002103022A2/en
Publication of WO2002103022A3 publication Critical patent/WO2002103022A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Abstract

The invention relates to transgenic cells which have been transformed with glucosyltransferase (GTase) nucleic acids which encode GTases capable of glucosylating p-hydroxybenzoic acid, or derivatives thereof.
PCT/GB2002/002610 2001-06-19 2002-06-18 Transgenic cells expressing clucosyltransferases which glucosylate parabenzoic acid WO2002103022A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002345150A AU2002345150A1 (en) 2001-06-19 2002-06-18 Transgenic cells expressing clucosyltransferases which glucosylate parabenzoic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0114855A GB0114855D0 (en) 2001-06-19 2001-06-19 Transgenic Cells
GB0114855.0 2001-06-19

Publications (2)

Publication Number Publication Date
WO2002103022A2 WO2002103022A2 (en) 2002-12-27
WO2002103022A3 true WO2002103022A3 (en) 2003-09-18

Family

ID=9916838

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/002610 WO2002103022A2 (en) 2001-06-19 2002-06-18 Transgenic cells expressing clucosyltransferases which glucosylate parabenzoic acid

Country Status (3)

Country Link
AU (1) AU2002345150A1 (en)
GB (1) GB0114855D0 (en)
WO (1) WO2002103022A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7662583B2 (en) 2003-05-27 2010-02-16 The University Of York Bioreactor containing cells expressing glycosyltransferase nucleic acids isolatable from Arabidopsis
US7056742B2 (en) 2003-06-16 2006-06-06 E. I. Du Pont De Nemours And Company High Level production of arbutin in green plants
US7846697B2 (en) 2003-06-19 2010-12-07 BLMaps Method of producing a low molecular weight organic compound in a cell

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
WO2002016655A2 (en) * 2000-08-24 2002-02-28 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
WO2002016655A2 (en) * 2000-08-24 2002-02-28 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BECHTHOLD A ET AL: "PARTIAL PURIFICATION PROPERTIES AND KINETIC STUDIES OF UDP-GLUCOSE P HYDROXYBENZOATE GLUCOSYLTRANSFERASE FROM CELL CULTURES OF LITHOSPERMUM-ERYTHRORHIZON", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 288, no. 1, 1991, pages 39 - 47, XP009009022, ISSN: 0003-9861 *
DATABASE EMBL [online] EBI; 22 March 2001 (2001-03-22), "Arabidopsis thaliana putative flavonol glucosyltransferase (At4g01070)", XP002237710, retrieved from EMBL Database accession no. AF360262 *
DATABASE EMBL [online] EBI; 24 June 1997 (1997-06-24), "O04622: similar to UTP-Glucose Glucosyltransferase", XP002237708, retrieved from EMBL Database accession no. AF007269 *
DATABASE EMBL [online] EBI; 27 September 2002 (2002-09-27), "Sequence 2692 from WO02/16655", XP002237709, retrieved from EMBL Database accession no. AX507997 *
LEE HYUNG-IL ET AL: "Purification, cloning, and expression of a pathogen inducible UDP-glucose: Salicylic acid glucosyltransferase from tobacco.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 51, 17 December 1999 (1999-12-17), pages 36637 - 36642, XP002237705, ISSN: 0021-9258 *
LI YI ET AL: "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 276, no. 6, 9 February 2001 (2001-02-09), pages 4338 - 4343, XP002172234, ISSN: 0021-9258 *
LIM ENG-KIAT ET AL: "The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 1, 4 January 2002 (2002-01-04), January 4, 2002, pages 586 - 592, XP002237707, ISSN: 0021-9258 *
ROSS J ET AL: "Higher plant glycosyltransferases.", GENOME BIOLOGY ENGLAND 2001, vol. 2, no. 2, 7 February 2001 (2001-02-07), pages REVIEWS3004, XP009008884, ISSN: 1465-6914 *

Also Published As

Publication number Publication date
AU2002345150A1 (en) 2003-01-02
GB0114855D0 (en) 2001-08-08
WO2002103022A2 (en) 2002-12-27

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