WO2002098824A3 - Improvements in enzyme stability - Google Patents

Improvements in enzyme stability Download PDF

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Publication number
WO2002098824A3
WO2002098824A3 PCT/AU2002/000746 AU0200746W WO02098824A3 WO 2002098824 A3 WO2002098824 A3 WO 2002098824A3 AU 0200746 W AU0200746 W AU 0200746W WO 02098824 A3 WO02098824 A3 WO 02098824A3
Authority
WO
WIPO (PCT)
Prior art keywords
cellulase
enzyme
amino acid
acid residue
elevated temperature
Prior art date
Application number
PCT/AU2002/000746
Other languages
French (fr)
Other versions
WO2002098824A2 (en
Inventor
Khawar Sohail Siddiqui
Ricardo Cavicchioli
Original Assignee
Unisearch Ltd
Khawar Sohail Siddiqui
Ricardo Cavicchioli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPR5532A external-priority patent/AUPR553201A0/en
Priority claimed from AUPR9689A external-priority patent/AUPR968901A0/en
Application filed by Unisearch Ltd, Khawar Sohail Siddiqui, Ricardo Cavicchioli filed Critical Unisearch Ltd
Priority to AU2002256572A priority Critical patent/AU2002256572A1/en
Publication of WO2002098824A2 publication Critical patent/WO2002098824A2/en
Publication of WO2002098824A3 publication Critical patent/WO2002098824A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

An enzyme for cleaving β-1,4 -glucosidic bonds of cellulose comprising a cellulase having an aromatic group linked to a side chain of an amino acid residue or to a terminal amino acid residue of the cellulase wherein the enzyme functions at an elevated temperature and/or has an extended half-life at elevated temperature compared to the corresponding unmodified cellulose.
PCT/AU2002/000746 2001-06-07 2002-06-07 Improvements in enzyme stability WO2002098824A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002256572A AU2002256572A1 (en) 2001-06-07 2002-06-07 Improvements in enzyme stability

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AUPR5532 2001-06-07
AUPR5532A AUPR553201A0 (en) 2001-06-07 2001-06-07 Improvements in enzyme stability
AUPR9689A AUPR968901A0 (en) 2001-12-21 2001-12-21 Improvements in enzyme stability
AUPR9689 2001-12-21

Publications (2)

Publication Number Publication Date
WO2002098824A2 WO2002098824A2 (en) 2002-12-12
WO2002098824A3 true WO2002098824A3 (en) 2003-01-30

Family

ID=25646721

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2002/000746 WO2002098824A2 (en) 2001-06-07 2002-06-07 Improvements in enzyme stability

Country Status (1)

Country Link
WO (1) WO2002098824A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010038501A1 (en) 2010-07-27 2012-02-02 Henkel Ag & Co. Kgaa Stabilized liquid enzyme-containing surfactant preparation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866526A (en) * 1993-10-04 1999-02-02 Novo Nordisk A/S Enzyme preparation comprising a modified enzyme

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866526A (en) * 1993-10-04 1999-02-02 Novo Nordisk A/S Enzyme preparation comprising a modified enzyme

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BOKHARI S. ET AL.: "Coupling of surface carboxyls of carboxymethylcellulase with aniline vis chemical modification: extreme thermostabilization in aqueous and water-miscible organic mixtures", BIOTECHNOL. PROG., vol. 18, no. 2, 2002, pages 276 - 281 *

Also Published As

Publication number Publication date
WO2002098824A2 (en) 2002-12-12

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