WO2002096928A3 - Peptide vector for transferring molecules of interest to eurocaryotic cells - Google Patents

Peptide vector for transferring molecules of interest to eurocaryotic cells Download PDF

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Publication number
WO2002096928A3
WO2002096928A3 PCT/FR2002/001772 FR0201772W WO02096928A3 WO 2002096928 A3 WO2002096928 A3 WO 2002096928A3 FR 0201772 W FR0201772 W FR 0201772W WO 02096928 A3 WO02096928 A3 WO 02096928A3
Authority
WO
WIPO (PCT)
Prior art keywords
interest
eurocaryotic
cells
peptide vector
transferring molecules
Prior art date
Application number
PCT/FR2002/001772
Other languages
French (fr)
Other versions
WO2002096928A2 (en
Inventor
Antoine Kichler
Christian Leborgne
Burkhard Bechinger
Original Assignee
Genethon
Max Planck Gesellschaft
Antoine Kichler
Christian Leborgne
Burkhard Bechinger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genethon, Max Planck Gesellschaft, Antoine Kichler, Christian Leborgne, Burkhard Bechinger filed Critical Genethon
Priority to AU2002314255A priority Critical patent/AU2002314255A1/en
Publication of WO2002096928A2 publication Critical patent/WO2002096928A2/en
Publication of WO2002096928A3 publication Critical patent/WO2002096928A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The invention relates to the use of a amphipathic cationic peptide vector having an absolute charge higher than or equal to pH2 to pH7,4 and comprising at least one hydrophilic portion, said portion comprising at least three residues which are capable of being protonized at a pH of less than 7, 4 in order to transfer a substance of interest to a eucaryotic cell.
PCT/FR2002/001772 2001-05-29 2002-05-27 Peptide vector for transferring molecules of interest to eurocaryotic cells WO2002096928A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002314255A AU2002314255A1 (en) 2001-05-29 2002-05-27 Peptide vector for transferring molecules of interest to eurocaryotic cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0106959A FR2825364A1 (en) 2001-05-29 2001-05-29 PEPTIIDIC VECTOR FOR THE TRANSFER OF NUCLEIC ACIDS IN EUKARYOTIC CELLS
FR01/06959 2001-05-29

Publications (2)

Publication Number Publication Date
WO2002096928A2 WO2002096928A2 (en) 2002-12-05
WO2002096928A3 true WO2002096928A3 (en) 2004-02-26

Family

ID=8863702

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/001772 WO2002096928A2 (en) 2001-05-29 2002-05-27 Peptide vector for transferring molecules of interest to eurocaryotic cells

Country Status (3)

Country Link
AU (1) AU2002314255A1 (en)
FR (1) FR2825364A1 (en)
WO (1) WO2002096928A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2639307T3 (en) * 2011-06-30 2017-10-26 Genethon Peptides with properties that enhance viral infection and their use
US9783576B2 (en) 2013-06-11 2017-10-10 Sanford-Burnham Medical Research Institute Compositions and methods for targeted endometriosis treatment

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993019768A1 (en) * 1992-04-03 1993-10-14 The Regents Of The University Of California Self-assembling polynucleotide delivery system
WO1995021931A1 (en) * 1994-02-08 1995-08-17 Rhone-Poulenc Rorer S.A. Nucleic acid-containing composition, its preparation and use
WO1998013026A1 (en) * 1997-06-10 1998-04-02 Genzyme Corporation Cationic amphiphile compositions for intracellular delivery of therapeutic molecules
WO1999006440A1 (en) * 1997-07-31 1999-02-11 Academisch Ziekenhuis Bij De Universiteit Van Amsterdam Novel synthetic peptides with antimicrobial and endotoxin neutralizing properties for management of the sepsis syndrome
WO1999042091A2 (en) * 1998-02-19 1999-08-26 Massachusetts Institute Of Technology Use of polycations as endosomolytic agents
EP1161957A1 (en) * 2000-05-26 2001-12-12 Transgene S.A. Complex for transferring an anionic substance of interest into a cell

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830730A (en) * 1997-05-08 1998-11-03 The Regents Of The University Of California Enhanced adenovirus-assisted transfection composition and method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993019768A1 (en) * 1992-04-03 1993-10-14 The Regents Of The University Of California Self-assembling polynucleotide delivery system
WO1995021931A1 (en) * 1994-02-08 1995-08-17 Rhone-Poulenc Rorer S.A. Nucleic acid-containing composition, its preparation and use
WO1998013026A1 (en) * 1997-06-10 1998-04-02 Genzyme Corporation Cationic amphiphile compositions for intracellular delivery of therapeutic molecules
WO1999006440A1 (en) * 1997-07-31 1999-02-11 Academisch Ziekenhuis Bij De Universiteit Van Amsterdam Novel synthetic peptides with antimicrobial and endotoxin neutralizing properties for management of the sepsis syndrome
WO1999042091A2 (en) * 1998-02-19 1999-08-26 Massachusetts Institute Of Technology Use of polycations as endosomolytic agents
EP1161957A1 (en) * 2000-05-26 2001-12-12 Transgene S.A. Complex for transferring an anionic substance of interest into a cell

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GOTTSCHALK S ET AL: "A NOVEL DNA-PEPTIDE COMPLEX FOR EFFICIENT GENE TRANSFER AND EXPRESSION IN MAMMALIAN CELLS", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 3, no. 5, 1 May 1996 (1996-05-01), pages 448 - 457, XP000577712, ISSN: 0969-7128 *
MIDOUX P AND MONSIGNY M: "Efficient gene transfer by histidylated polylysine/pDNA complexes", BIOCONJUG CHEM, vol. 10, no. 3, May 1999 (1999-05-01), pages 406 - 411, XP002191011 *
MIDOUX P ET AL: "Membrane permeabilization and efficient gene transfer by a peptide containing several histidines", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 9, no. 2, March 1998 (1998-03-01), pages 260 - 267, XP002108845, ISSN: 1043-1802 *
MIDOUX P ET AL: "SPECIFIC GENE TRANSFER MEDIATED BY LACTOSYLATED POLY-L-LYSINE INTO HEPATOMA CELLS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 4, 25 February 1993 (1993-02-25), pages 871 - 878, XP000371764, ISSN: 0305-1048 *
VOGT T ET AL: "The interactions of histidine-containing amphipathic helical peptide antibiotics with lipid bilayers. The effects of charges and pH", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 274, no. 41, 8 October 1999 (1999-10-08), pages 29115 - 29121, XP001053772 *
WYMAN ET AL: "DESIGN, SYNTHESIS, AND CHARACTERIZATION OF A CATIONIC PEPTIDE THAT BINDS TO NUCLEIC ACIDS AND PERMEABILIZES BILAYERS", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 36, 1997, pages 3008 - 3017, XP002088731, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
WO2002096928A2 (en) 2002-12-05
AU2002314255A1 (en) 2002-12-09
FR2825364A1 (en) 2002-12-06

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