WO2002095419A3 - Peptide analysis using a solid support - Google Patents

Peptide analysis using a solid support Download PDF

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Publication number
WO2002095419A3
WO2002095419A3 PCT/US2002/016247 US0216247W WO02095419A3 WO 2002095419 A3 WO2002095419 A3 WO 2002095419A3 US 0216247 W US0216247 W US 0216247W WO 02095419 A3 WO02095419 A3 WO 02095419A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
solid support
identifying
peptide analysis
peptide
Prior art date
Application number
PCT/US2002/016247
Other languages
French (fr)
Other versions
WO2002095419A2 (en
Inventor
Ramagauri Bhikhabhai
Maria Liminga
Jean-Luc Maloisel
Ronnie Palmgren
Thomas W Keough
Robert S Youngquist
Harold L Vaughn
Ken E Yelm
Original Assignee
Amersham Biosciences Ab
Procter & Gamble
Ramagauri Bhikhabhai
Maria Liminga
Jean-Luc Maloisel
Ronnie Palmgren
Thomas W Keough
Robert S Youngquist
Harold L Vaughn
Ken E Yelm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/863,786 external-priority patent/US7074570B2/en
Application filed by Amersham Biosciences Ab, Procter & Gamble, Ramagauri Bhikhabhai, Maria Liminga, Jean-Luc Maloisel, Ronnie Palmgren, Thomas W Keough, Robert S Youngquist, Harold L Vaughn, Ken E Yelm filed Critical Amersham Biosciences Ab
Priority to JP2002591841A priority Critical patent/JP2005518521A/en
Priority to AU2002311995A priority patent/AU2002311995A1/en
Priority to EP02739342A priority patent/EP1434999A2/en
Priority to US10/478,910 priority patent/US20040171070A1/en
Priority to CA002448534A priority patent/CA2448534A1/en
Publication of WO2002095419A2 publication Critical patent/WO2002095419A2/en
Publication of WO2002095419A3 publication Critical patent/WO2002095419A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.
PCT/US2002/016247 2001-05-23 2002-05-20 Peptide analysis using a solid support WO2002095419A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2002591841A JP2005518521A (en) 2001-05-23 2002-05-20 Peptide analysis using solid supports
AU2002311995A AU2002311995A1 (en) 2001-05-23 2002-05-20 Peptide analysis using a solid support
EP02739342A EP1434999A2 (en) 2001-05-23 2002-05-20 Peptide analysis using a solid support
US10/478,910 US20040171070A1 (en) 2002-05-20 2002-05-20 Peptide analysis using a solid support
CA002448534A CA2448534A1 (en) 2001-05-23 2002-05-20 Peptide analysis using a solid support

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/863,786 US7074570B2 (en) 2001-05-23 2001-05-23 Peptide fragmentation
US09/863,786 2001-05-23
US34301901P 2001-12-20 2001-12-20
US60/343,019 2001-12-20

Publications (2)

Publication Number Publication Date
WO2002095419A2 WO2002095419A2 (en) 2002-11-28
WO2002095419A3 true WO2002095419A3 (en) 2003-04-24

Family

ID=26993298

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/016247 WO2002095419A2 (en) 2001-05-23 2002-05-20 Peptide analysis using a solid support

Country Status (5)

Country Link
EP (1) EP1434999A2 (en)
JP (1) JP2005518521A (en)
AU (1) AU2002311995A1 (en)
CA (1) CA2448534A1 (en)
WO (1) WO2002095419A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPR116100A0 (en) 2000-11-01 2000-11-23 University Of Sydney, The P450 gene regulation
GB0320415D0 (en) * 2003-08-30 2003-10-01 Shimadzu Res Lab Europe Ltd Analysis of macromolecules
US7371514B2 (en) 2004-07-16 2008-05-13 Agilent Technologies, Inc. Serial derivatization of peptides for de novo sequencing using tandem mass spectrometry
WO2008074067A1 (en) * 2006-12-18 2008-06-26 Macquarie University Detection and quantification of polypeptides using mass spectrometry
HRP20100044B1 (en) 2010-01-25 2016-11-18 Institut Ruđer Bošković Protein identification using mass spectrometry
JP7070671B2 (en) * 2018-04-16 2022-05-18 株式会社島津製作所 Mass spectrometric kit, microbial identification kit, sample preparation method, analysis method and microbial identification method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3478013A (en) * 1966-08-22 1969-11-11 Abbott Lab Carbohydrate derivatives of erythromycin
US4296109A (en) * 1978-10-04 1981-10-20 Schering, Aktiengesellschaft Corticoid 21-sulfopropionates and the salts thereof, a process for the production thereof and pharmaceutical preparations thereof
WO2000043792A2 (en) * 1999-01-20 2000-07-27 The Procter & Gamble Company Methods and kits for sequencing polypeptides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3478013A (en) * 1966-08-22 1969-11-11 Abbott Lab Carbohydrate derivatives of erythromycin
US4296109A (en) * 1978-10-04 1981-10-20 Schering, Aktiengesellschaft Corticoid 21-sulfopropionates and the salts thereof, a process for the production thereof and pharmaceutical preparations thereof
WO2000043792A2 (en) * 1999-01-20 2000-07-27 The Procter & Gamble Company Methods and kits for sequencing polypeptides

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL BIOCHEMISTRY, vol. 108, no. 2, 1980, pages 394 - 401, ISSN: 0003-2697 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1980, FINK G ET AL: "REAGENTS SUITABLE FOR THE CROSS LINKING OF NUCLEIC-ACIDS TO PROTEINS", XP002227343, Database accession no. PREV198171050036 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1983, KEANA J F W ET AL: "DETERGENTS CONTAINING A 1 3 DIENE GROUP IN THE HYDROPHOBIC SEGMENT FACILE CHEMICAL MODIFICATION BY A DIELS ALDER REACTION WITH HYDROPHILIC DIENOPHILES IN AQUEOUS SOLUTION", XP002227344, Database accession no. PREV198477032524 *
JOURNAL OF ORGANIC CHEMISTRY, vol. 48, no. 16, 1983, pages 2661 - 2666, ISSN: 0022-3263 *
KEOUGH ET AL: "A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionization mass spectrometry", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, June 1999 (1999-06-01), pages 7131 - 7136, XP002141709, ISSN: 0027-8424 *
KEOUGH T ET AL: "Solid-phase derivatization of tryptic peptides for rapid protein identification by matrix-assisted laser desorption/ionization mass spectrometry.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 16, no. 11, 2002, pages 1003 - 1015, XP009004084, ISSN: 0951-4198 *

Also Published As

Publication number Publication date
JP2005518521A (en) 2005-06-23
EP1434999A2 (en) 2004-07-07
AU2002311995A1 (en) 2002-12-03
WO2002095419A2 (en) 2002-11-28
CA2448534A1 (en) 2002-11-28

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