WO2002092794A3 - Isolation method of mesenchymal cells - Google Patents

Isolation method of mesenchymal cells

Info

Publication number
WO2002092794A3
WO2002092794A3 PCT/NL2002/000305 NL0200305W WO02092794A3 WO 2002092794 A3 WO2002092794 A3 WO 2002092794A3 NL 0200305 W NL0200305 W NL 0200305W WO 02092794 A3 WO02092794 A3 WO 02092794A3
Authority
WO
WIPO (PCT)
Prior art keywords
mesenchymal cells
isolation method
digest
dermal tissue
centrifuging
Prior art date
Application number
PCT/NL2002/000305
Other languages
French (fr)
Other versions
WO2002092794A2 (en
Inventor
Marion Bertrand-De-Haas
Evert Nicolaas Lamme
Blitterswijk Clemens Anton Van
Original Assignee
Isotis Nv
Marion Bertrand-De-Haas
Evert Nicolaas Lamme
Blitterswijk Clemens Anton Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isotis Nv, Marion Bertrand-De-Haas, Evert Nicolaas Lamme, Blitterswijk Clemens Anton Van filed Critical Isotis Nv
Priority to AU2002309330A priority Critical patent/AU2002309330A1/en
Publication of WO2002092794A2 publication Critical patent/WO2002092794A2/en
Publication of WO2002092794A3 publication Critical patent/WO2002092794A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a method for isolating mesenchymal cells from dermal tissue comprising the steps of : - incubating dermal tissue in a buffer containing collagenase type II to produce a digest; - filtering the digest; and - centrifuging the digest.
PCT/NL2002/000305 2001-05-17 2002-05-10 Isolation method of mesenchymal cells WO2002092794A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002309330A AU2002309330A1 (en) 2001-05-17 2002-05-10 Isolation method of mesenchymal cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01201863 2001-05-17
EP01201863.6 2001-05-17

Publications (2)

Publication Number Publication Date
WO2002092794A2 WO2002092794A2 (en) 2002-11-21
WO2002092794A3 true WO2002092794A3 (en) 2003-02-13

Family

ID=8180329

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2002/000305 WO2002092794A2 (en) 2001-05-17 2002-05-10 Isolation method of mesenchymal cells

Country Status (2)

Country Link
AU (1) AU2002309330A1 (en)
WO (1) WO2002092794A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2384618C2 (en) * 2008-03-27 2010-03-20 Общество С Ограниченной Ответственностью "Лаборатория Клеточных Технологий" Method for making fibroblast-like cells of umbilical cord of newborn
US9677042B2 (en) 2010-10-08 2017-06-13 Terumo Bct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US9617506B2 (en) 2013-11-16 2017-04-11 Terumo Bct, Inc. Expanding cells in a bioreactor
EP3613841B1 (en) 2014-03-25 2022-04-20 Terumo BCT, Inc. Passive replacement of media
EP3198006B1 (en) 2014-09-26 2021-03-24 Terumo BCT, Inc. Scheduled feed
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
EP3656842A1 (en) 2017-03-31 2020-05-27 Terumo BCT, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424208A (en) * 1993-04-16 1995-06-13 Baxter International Inc. Method for isolating cells from tissue with a composition containing collagenase and chymopapin
WO1996000283A1 (en) * 1994-06-24 1996-01-04 Boehringer Mannheim Corporation A purified mixture of collagenases and two other proteases obtained from clostridium histolyticum
US5830741A (en) * 1996-12-06 1998-11-03 Boehringer Mannheim Corporation Composition for tissue dissociation containing collagenase I and II from clostridium histolyticum and a neutral protease
WO2001025402A1 (en) * 1999-10-06 2001-04-12 Tigenix N.V. Isolation of precursor cells and their use for tissue repair
WO2001032129A2 (en) * 1999-11-05 2001-05-10 Gerigene Medical Corporation Augmentation and repair of age-related soft tissue defects

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424208A (en) * 1993-04-16 1995-06-13 Baxter International Inc. Method for isolating cells from tissue with a composition containing collagenase and chymopapin
WO1996000283A1 (en) * 1994-06-24 1996-01-04 Boehringer Mannheim Corporation A purified mixture of collagenases and two other proteases obtained from clostridium histolyticum
US5830741A (en) * 1996-12-06 1998-11-03 Boehringer Mannheim Corporation Composition for tissue dissociation containing collagenase I and II from clostridium histolyticum and a neutral protease
WO2001025402A1 (en) * 1999-10-06 2001-04-12 Tigenix N.V. Isolation of precursor cells and their use for tissue repair
WO2001032129A2 (en) * 1999-11-05 2001-05-10 Gerigene Medical Corporation Augmentation and repair of age-related soft tissue defects

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KLAGSBRUN M.: "Large-scale Preparation of Chondrocytes", METHODS IN ENZYMOLOGY, vol. LVIII, 1979, pages 560 - 564, XP001069943 *
STENN K S ET AL: "DISPASE A NEUTRAL PROTEASE FROM BACILLUS-POLYMYXA IS A POWERFUL FIBRONECTINASE AND TYPE IV COLLAGENASE", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 93, no. 2, 1989, pages 287 - 290, XP001070905, ISSN: 0022-202X *

Also Published As

Publication number Publication date
WO2002092794A2 (en) 2002-11-21
AU2002309330A1 (en) 2002-11-25

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