WO2002092757A3 - Enzyme over-producing transgenic microorganisms - Google Patents

Enzyme over-producing transgenic microorganisms Download PDF

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Publication number
WO2002092757A3
WO2002092757A3 PCT/US2001/044233 US0144233W WO02092757A3 WO 2002092757 A3 WO2002092757 A3 WO 2002092757A3 US 0144233 W US0144233 W US 0144233W WO 02092757 A3 WO02092757 A3 WO 02092757A3
Authority
WO
WIPO (PCT)
Prior art keywords
galactosidase
produce
supernatant
dried
producing transgenic
Prior art date
Application number
PCT/US2001/044233
Other languages
French (fr)
Other versions
WO2002092757A2 (en
Inventor
Donald M Mattson Jr
Friedhelm Brinkhaus
Randy L Peters
Steven Siembieda
Michelle P Moore
Nugzar N Nutsibidze
Original Assignee
Kemin Ind Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kemin Ind Inc filed Critical Kemin Ind Inc
Priority to AU2001297819A priority Critical patent/AU2001297819A1/en
Publication of WO2002092757A2 publication Critical patent/WO2002092757A2/en
Publication of WO2002092757A3 publication Critical patent/WO2002092757A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A strain of Trichoderma reesei transformed to over-produce α-galactosidase. PCR primers are designed and used to link the α-galactosidase gene to the cellobiohydrolase promoter and terminator so that the α-galactosidase is expressed and expelled from the organism. Fermentation of the transformed organism produces α-galactosidase in the supernatant that is at a level of a least 20 IU per ml. Use of soybean hulls as an inducer increases the amount of α-galactosidase produced. The supernatant is filtered and concentrated to produce a liquid product that may be added to feed and food to improve conversion. The liquid concentrate may be dried, for example by spray drying, to produce a dried α-galactosidase product with an extended shelf life.
PCT/US2001/044233 2000-11-15 2001-11-14 Enzyme over-producing transgenic microorganisms WO2002092757A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001297819A AU2001297819A1 (en) 2000-11-15 2001-11-14 Enzyme over-producing transgenic microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71358800A 2000-11-15 2000-11-15
US09/713,588 2000-11-15

Publications (2)

Publication Number Publication Date
WO2002092757A2 WO2002092757A2 (en) 2002-11-21
WO2002092757A3 true WO2002092757A3 (en) 2003-03-13

Family

ID=24866707

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/044233 WO2002092757A2 (en) 2000-11-15 2001-11-14 Enzyme over-producing transgenic microorganisms

Country Status (3)

Country Link
CN (1) CN1524120A (en)
AU (1) AU2001297819A1 (en)
WO (1) WO2002092757A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5192700B2 (en) * 2007-01-19 2013-05-08 サントリーホールディングス株式会社 Novel glycosylation enzyme and polynucleotide encoding the same
JP2010520323A (en) * 2007-02-28 2010-06-10 ダニスコ・ユーエス・インク Detergent composition containing alpha-galactosidase
US8093016B2 (en) 2007-05-21 2012-01-10 Danisco Us Inc. Use of an aspartic protease (NS24) signal sequence for heterologous protein expression
US8450098B2 (en) * 2007-05-21 2013-05-28 Danisco Us Inc. Method for introducing nucleic acids into fungal cells
CN103031289A (en) * 2012-11-30 2013-04-10 青岛蔚蓝生物集团有限公司 Lactase and recombinant expression engineering bacterium thereof
CN116334111B (en) * 2023-03-03 2024-02-09 上海市农业科学院 Straw mushroom cellobiose hydrolase and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634670A (en) * 1983-07-27 1987-01-06 Shin Nenryoyu Kaihatsu Gijutsu Kenkyu Kumiai Process for the preparation of cellulase
WO1998023764A1 (en) * 1996-11-29 1998-06-04 Röhm Enzyme Finland OY Truncated cbh i promoter from trichoderma reesei and use thereof
US5919690A (en) * 1993-03-31 1999-07-06 Novo Nordisk A/S α-galactosidase enzyme
US6090574A (en) * 1989-07-07 2000-07-18 Unilever Patent Holdings, B.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634670A (en) * 1983-07-27 1987-01-06 Shin Nenryoyu Kaihatsu Gijutsu Kenkyu Kumiai Process for the preparation of cellulase
US6090574A (en) * 1989-07-07 2000-07-18 Unilever Patent Holdings, B.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
US5919690A (en) * 1993-03-31 1999-07-06 Novo Nordisk A/S α-galactosidase enzyme
WO1998023764A1 (en) * 1996-11-29 1998-06-04 Röhm Enzyme Finland OY Truncated cbh i promoter from trichoderma reesei and use thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MARGOLLES-CLARK ET AL.: "Three alpha-galactosidase genes of trichoderma reesei cloned by expression in yeast", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 240, 1996, pages 104 - 111, XP001052713 *
PALOHEIMO ET AL.: "Enzyme production by trichoderma reesei using the cbh1 promoter", PROCEEDINGS OF THE SECOND TRICEL SYMPOSIUM ON TRICHODERMA REESEI CELLULASES AND OTHER HYDROLASES (EDITED BY P. SULOMINEN & T. REINIKAINEN, FOUNDATION FOR BIOTECHNICAL AND INDUSTRIAL FERMENTATION RESEARCH), vol. 8, 1993, ESPOO, FINLAND, pages 229 - 238, XP001069763 *

Also Published As

Publication number Publication date
AU2001297819A1 (en) 2002-11-25
WO2002092757A2 (en) 2002-11-21
CN1524120A (en) 2004-08-25

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