WO2002090992A3 - Screening method - Google Patents

Screening method Download PDF

Info

Publication number
WO2002090992A3
WO2002090992A3 PCT/GB2002/001946 GB0201946W WO02090992A3 WO 2002090992 A3 WO2002090992 A3 WO 2002090992A3 GB 0201946 W GB0201946 W GB 0201946W WO 02090992 A3 WO02090992 A3 WO 02090992A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell array
agents
trap
genes
identification
Prior art date
Application number
PCT/GB2002/001946
Other languages
French (fr)
Other versions
WO2002090992A2 (en
Inventor
Peter Andrews
Jon Draper
James Walsh
Original Assignee
Axordia Ltd
Peter Andrews
Jon Draper
James Walsh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axordia Ltd, Peter Andrews, Jon Draper, James Walsh filed Critical Axordia Ltd
Priority to AU2002249467A priority Critical patent/AU2002249467A1/en
Publication of WO2002090992A2 publication Critical patent/WO2002090992A2/en
Publication of WO2002090992A3 publication Critical patent/WO2002090992A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A screening method is disclosed which enables the identification of biologically active agents which mediate their effects through the activation of genes. The method utilises promoter trap, enhancer trap and gene trap constructs, comprising a reporter molecule, which are transfected into cells which are then cloned into a cell array. The cell array is exposed to an agent(s) to be tested and the response of the cell array to the agent monitored. The method enables both the identification of agents and the genes through which the agents act.
PCT/GB2002/001946 2001-05-04 2002-04-29 Screening method WO2002090992A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002249467A AU2002249467A1 (en) 2001-05-04 2002-04-29 Screening method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0111004.8A GB0111004D0 (en) 2001-05-04 2001-05-04 Screening method
GB0111004.8 2001-05-04

Publications (2)

Publication Number Publication Date
WO2002090992A2 WO2002090992A2 (en) 2002-11-14
WO2002090992A3 true WO2002090992A3 (en) 2003-12-04

Family

ID=9914069

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/001946 WO2002090992A2 (en) 2001-05-04 2002-04-29 Screening method

Country Status (3)

Country Link
AU (1) AU2002249467A1 (en)
GB (1) GB0111004D0 (en)
WO (1) WO2002090992A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2453804A1 (en) 2001-07-17 2003-04-10 Massachusetts Institute Of Technology Unique properties of stem cells
US7193069B2 (en) 2002-03-22 2007-03-20 Research Association For Biotechnology Full-length cDNA
DE10319478A1 (en) * 2003-04-30 2004-11-18 Universität Zu Köln siRNA selection process
US7618936B2 (en) 2004-05-21 2009-11-17 The Regents Of The University Of California Methods for treating and diagnosing cancer with WNT inhibitory Factor-1 (WIF-1)
US9045553B2 (en) 2004-05-27 2015-06-02 Acceleron Pharma, Inc. Cerberus/Coco derivatives and uses thereof
US20100183695A1 (en) 2004-05-27 2010-07-22 John Knopf Cerberus/coco derivatives and uses thereof
WO2005121323A2 (en) 2004-06-07 2005-12-22 Massachusetts Institute Of Technology Methods for ex vivo propagation of somatic hair follicle stem cells
US20080152632A1 (en) * 2005-06-22 2008-06-26 Roslin Institute Promoter-reporter cells for determining drug metabolism, drug interactions, and the effects of allotype variation
WO2007002228A1 (en) 2005-06-23 2007-01-04 Massachusetts Institute Of Technology Methods for ex vivo propagation of adult hepatic stem cells
EP2099471A2 (en) 2006-12-08 2009-09-16 Acceleron Pharma, Inc. Uses of cerberus, coco and derivatives thereof
US20160160224A1 (en) * 2013-08-02 2016-06-09 University Of Georgia Research Foundation, Inc. Cryptosporidium transfection methods and transfected cryptosporidium cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO2001007891A2 (en) * 1999-07-27 2001-02-01 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
WO2001019855A2 (en) * 1999-09-13 2001-03-22 American Home Products Corporation Pharmaceutical compositions and methods of using secreted frizzled related protein

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO2001007891A2 (en) * 1999-07-27 2001-02-01 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
WO2001019855A2 (en) * 1999-09-13 2001-03-22 American Home Products Corporation Pharmaceutical compositions and methods of using secreted frizzled related protein

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BAFICO ANNA ET AL: "Interaction of frizzled related protein (FRP) with Wnt ligands and the frizzled receptor suggests alternative mechanisms for FRP inhibition of Wnt signaling.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 23, 4 June 1999 (1999-06-04), pages 16180 - 16187, XP002243190, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2002090992A2 (en) 2002-11-14
GB0111004D0 (en) 2001-06-27
AU2002249467A1 (en) 2002-11-18

Similar Documents

Publication Publication Date Title
WO2002090992A3 (en) Screening method
WO2002077264A3 (en) Arrayed transfection method and uses related thereto
DE60031997D1 (en) UMKEHRTRANSFEKTIONSVERFAHREN
DE60014166T2 (en) METHOD FOR MEASURING DEACETYLASE ACTIVITY AND METHOD FOR SCREENING INHIBITORS OR PROMOTERS OF THIS ENZYME
WO2001019859A3 (en) LEPIDOPTERAN-ACTIVE BACILLUS THURINGIENSIS δ-ENDOTOXIN COMPOSITIONS AND METHODS OF USE
ATE260989T1 (en) INSIGHTS FROM GENOME RESEARCH FOR THE SEARCH FOR NOVEL ACTIVE INGREDIENTS
WO2005022113A3 (en) Ion channel assay methods
AU4007401A (en) Function homology screening
EP1801220A3 (en) Insect-resistant transgenic plants and methods for improving delta-endotoxin activity against insects
WO2002074969A3 (en) Matrix attachment regions and methods for use thereof
PT981637E (en) METHOD FOR INTEGRATING GENES IN SPECIFIC SITES IN MAMIFERO CELLS THROUGH RECOMBINATION APPROVAL AND VECTORS FOR THE REALIZATION OF THE SAME
DK1244808T3 (en) Method for screening multiple transformed cells using bicistronic expression of fluorescent proteins
AU1265097A (en) Nucleic acid constructs for the cell cycle-regulated expression of genes, cells containing such constructs and their use for preparing drugs
DK1066392T3 (en) Vectors for gene mutagenesis and gene discovery
WO2005003315A3 (en) Compositions and methods for peptide-assisted transfection
CA2356458A1 (en) Plastid transformation of brassica
Liu et al. Direct electrochemistry of hemoglobin in dimethyldioctadecyl ammonium bromide film and its electrocatalysis to nitric oxide
GB9720693D0 (en) Assay
AU3107699A (en) Novel forms of t cell costimulatory proteins, nucleic acid molecules, and uses thereof
WO2003006947A3 (en) Cell-screening assay and composition
CA2411649A1 (en) Ires enabled gene trapping in plants
WO2004108898A3 (en) Transfection agents
WO2000061809A3 (en) Methods and nucleic acid vectors for rapid and parallel assay development, for characterization of biological response modifiers
CN108717076A (en) A kind of electrochemica biological sensor and preparation method thereof of detection zeatin
HK1063645A1 (en) Bioassay for myostatin

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP