WO2002090947A3 - Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und verfahren zur fluoreszenzfluktuationsmessung sowie verfahren und vorrichtung zur justage eines fluoreszenzfluktuationsmikroskops - Google Patents

Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und verfahren zur fluoreszenzfluktuationsmessung sowie verfahren und vorrichtung zur justage eines fluoreszenzfluktuationsmikroskops Download PDF

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Publication number
WO2002090947A3
WO2002090947A3 PCT/DE2002/001649 DE0201649W WO02090947A3 WO 2002090947 A3 WO2002090947 A3 WO 2002090947A3 DE 0201649 W DE0201649 W DE 0201649W WO 02090947 A3 WO02090947 A3 WO 02090947A3
Authority
WO
WIPO (PCT)
Prior art keywords
microscope
fluorescence fluctuation
fluorescence
fluctuation
module
Prior art date
Application number
PCT/DE2002/001649
Other languages
English (en)
French (fr)
Other versions
WO2002090947A2 (de
WO2002090947A8 (de
Inventor
Joerg Langowski
Malte Wachsmuth
Original Assignee
Deutsches Krebsforsch
Joerg Langowski
Malte Wachsmuth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforsch, Joerg Langowski, Malte Wachsmuth filed Critical Deutsches Krebsforsch
Priority to EP02724136A priority Critical patent/EP1386139A2/de
Priority to DE10291983T priority patent/DE10291983D2/de
Priority to US10/477,153 priority patent/US20040238730A1/en
Publication of WO2002090947A2 publication Critical patent/WO2002090947A2/de
Publication of WO2002090947A3 publication Critical patent/WO2002090947A3/de
Publication of WO2002090947A8 publication Critical patent/WO2002090947A8/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/024—Modular construction

Landscapes

  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

Ein Fluoreszenzfluktuationsmikroskop, bei welchem Anregungslicht und Detektionslicht über einen gemeinsamen Strahlengang in einem Mikroskop eingekoppelt bzw. aus diesem ausgekoppelt werden, weist eine closed-loop-Scanning-Einheit (22, 23) auf.
PCT/DE2002/001649 2001-05-07 2002-05-07 Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und verfahren zur fluoreszenzfluktuationsmessung sowie verfahren und vorrichtung zur justage eines fluoreszenzfluktuationsmikroskops WO2002090947A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02724136A EP1386139A2 (de) 2001-05-07 2002-05-07 Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und verfahren zur fluoreszenzfluktuationsmessung sowie verfahren und vorrichtung zur justage eines fluoreszenzfluktuationsmikroskops
DE10291983T DE10291983D2 (de) 2001-05-07 2002-05-07 Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und Verfahren zur Fluoreszenzfluktuationsmessung sowie Verfahren und Vorrichtung zur Justage eines Fluoreszenzfluktuationsmikroskops
US10/477,153 US20040238730A1 (en) 2001-05-07 2002-05-07 Fluorescence fluctuation microscope analytical module or scanning module, method for measurement of fluorescence fluctuation and method and device for adjustment of a fluorescence fluctuation microscope

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10122046.4 2001-05-07
DE10122046 2001-05-07

Publications (3)

Publication Number Publication Date
WO2002090947A2 WO2002090947A2 (de) 2002-11-14
WO2002090947A3 true WO2002090947A3 (de) 2003-04-10
WO2002090947A8 WO2002090947A8 (de) 2005-03-17

Family

ID=7683852

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/001649 WO2002090947A2 (de) 2001-05-07 2002-05-07 Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und verfahren zur fluoreszenzfluktuationsmessung sowie verfahren und vorrichtung zur justage eines fluoreszenzfluktuationsmikroskops

Country Status (4)

Country Link
US (1) US20040238730A1 (de)
EP (1) EP1386139A2 (de)
DE (1) DE10291983D2 (de)
WO (1) WO2002090947A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10327531B4 (de) * 2003-06-17 2006-11-30 Leica Microsystems Cms Gmbh Verfahren zur Messung von Fluoreszenzkorrelationen in Gegenwart von langsamen Signalschwankungen
DE102009013147A1 (de) * 2009-03-05 2010-09-16 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Spektroskopie heterogener Proben
JP6800845B2 (ja) * 2014-10-29 2020-12-16 ルーメンコア インコーポレイテッド 統合型蛍光スキャニングシステム
JP2020502558A (ja) 2016-11-10 2020-01-23 ザ トラスティーズ オブ コロンビア ユニバーシティ イン ザ シティ オブ ニューヨーク 大型試料のための高速・高解像度イメージング方法

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995030167A1 (en) * 1994-04-30 1995-11-09 Medical Research Council Scanning confocal optical microscope
WO1997011355A1 (en) * 1995-09-19 1997-03-27 Cornell Research Foundation, Inc. Multi-photon laser microscopy
DE19733195A1 (de) * 1997-08-01 1999-02-04 Zeiss Carl Jena Gmbh Hoch-Kompaktes Laser-Scanning-Mikroskop
WO1999047041A1 (en) * 1998-03-19 1999-09-23 Board Of Regents, The University Of Texas System Fiber-optic confocal imaging apparatus and methods of use
US6057546A (en) * 1997-02-28 2000-05-02 Thermomicroscopes Corp. Kinematically mounted probe holder for scanning probe microscope
US6072624A (en) * 1992-01-09 2000-06-06 Biomedical Photometrics Inc. Apparatus and method for scanning laser imaging of macroscopic samples
DE19910955A1 (de) * 1999-03-12 2000-09-28 Evotec Analytical Sys Gmbh Chemosensitivitätsmessung über Phosphatidylserin
US6134002A (en) * 1999-01-14 2000-10-17 Duke University Apparatus and method for the rapid spectral resolution of confocal images
WO2001008081A1 (en) * 1999-07-21 2001-02-01 Surromed, Inc. System for microvolume laser scanning cytometry

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035476A (en) * 1990-06-15 1991-07-30 Hamamatsu Photonics K.K. Confocal laser scanning transmission microscope
US5535052A (en) * 1992-07-24 1996-07-09 Carl-Zeiss-Stiftung Laser microscope
DE59405644D1 (de) * 1993-01-18 1998-05-14 Evotec Biosystems Gmbh Verfahren und vorrichtung zur bewertung der fitness von biopolymeren
EP0734539B1 (de) * 1994-08-25 2002-11-13 Leica Microsystems Heidelberg GmbH Vorrichtung zum einkoppeln des lichtstrahls eines uv-lasers in ein laser-scanmikroskop
US5981956A (en) * 1996-05-16 1999-11-09 Affymetrix, Inc. Systems and methods for detection of labeled materials
DE19649605A1 (de) * 1996-11-29 1998-06-04 Deutsches Krebsforsch Fluoreszenzkorrelationsspektroskopiemodul für ein Mikroskop
US6376843B1 (en) * 1999-06-23 2002-04-23 Evotec Oai Ag Method of characterizing fluorescent molecules or other particles using generating functions
US6603546B1 (en) * 2000-07-21 2003-08-05 I.S.S. (Usa) Inc. Rapid high throughput spectrometer and method

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6072624A (en) * 1992-01-09 2000-06-06 Biomedical Photometrics Inc. Apparatus and method for scanning laser imaging of macroscopic samples
WO1995030167A1 (en) * 1994-04-30 1995-11-09 Medical Research Council Scanning confocal optical microscope
WO1997011355A1 (en) * 1995-09-19 1997-03-27 Cornell Research Foundation, Inc. Multi-photon laser microscopy
US6057546A (en) * 1997-02-28 2000-05-02 Thermomicroscopes Corp. Kinematically mounted probe holder for scanning probe microscope
DE19733195A1 (de) * 1997-08-01 1999-02-04 Zeiss Carl Jena Gmbh Hoch-Kompaktes Laser-Scanning-Mikroskop
WO1999047041A1 (en) * 1998-03-19 1999-09-23 Board Of Regents, The University Of Texas System Fiber-optic confocal imaging apparatus and methods of use
US6134002A (en) * 1999-01-14 2000-10-17 Duke University Apparatus and method for the rapid spectral resolution of confocal images
DE19910955A1 (de) * 1999-03-12 2000-09-28 Evotec Analytical Sys Gmbh Chemosensitivitätsmessung über Phosphatidylserin
WO2001008081A1 (en) * 1999-07-21 2001-02-01 Surromed, Inc. System for microvolume laser scanning cytometry

Also Published As

Publication number Publication date
EP1386139A2 (de) 2004-02-04
DE10291983D2 (de) 2004-04-15
US20040238730A1 (en) 2004-12-02
WO2002090947A2 (de) 2002-11-14
WO2002090947A8 (de) 2005-03-17

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