WO2002090550A3 - Method for preparing recombinant poxvirus - Google Patents

Method for preparing recombinant poxvirus Download PDF

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Publication number
WO2002090550A3
WO2002090550A3 PCT/GB2002/002190 GB0202190W WO02090550A3 WO 2002090550 A3 WO2002090550 A3 WO 2002090550A3 GB 0202190 W GB0202190 W GB 0202190W WO 02090550 A3 WO02090550 A3 WO 02090550A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant poxvirus
preparing recombinant
cell sorting
activated cell
fluorescence activated
Prior art date
Application number
PCT/GB2002/002190
Other languages
French (fr)
Other versions
WO2002090550A2 (en
Inventor
Sarah Gilbert
Original Assignee
Isis Innovation
Sarah Gilbert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation, Sarah Gilbert filed Critical Isis Innovation
Priority to AU2002307938A priority Critical patent/AU2002307938A1/en
Publication of WO2002090550A2 publication Critical patent/WO2002090550A2/en
Publication of WO2002090550A3 publication Critical patent/WO2002090550A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24161—Methods of inactivation or attenuation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to use of fluorescence activated cell sorting in the production of recombinant poxvirus, in particular to the use of fluorescence activated cell sorting in the separation of cells containing recombinant poxvirus from cells not containing recombinant poxvirus.
PCT/GB2002/002190 2001-05-10 2002-05-10 Method for preparing recombinant poxvirus WO2002090550A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002307938A AU2002307938A1 (en) 2001-05-10 2002-05-10 Method for preparing recombinant poxvirus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0111442A GB0111442D0 (en) 2001-05-10 2001-05-10 Method for preparing recombinant virus
GB0111442.0 2001-05-10

Publications (2)

Publication Number Publication Date
WO2002090550A2 WO2002090550A2 (en) 2002-11-14
WO2002090550A3 true WO2002090550A3 (en) 2003-05-22

Family

ID=9914386

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/002190 WO2002090550A2 (en) 2001-05-10 2002-05-10 Method for preparing recombinant poxvirus

Country Status (3)

Country Link
AU (1) AU2002307938A1 (en)
GB (1) GB0111442D0 (en)
WO (1) WO2002090550A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1594970T3 (en) * 2003-02-18 2008-11-17 Helmholtz Zentrum Muenchen Method for Generating Recombinant MVA
US10663468B2 (en) * 2015-07-24 2020-05-26 Boehringer Ingelheim Animal Health USA Inc. High throughput methods for virus quantification

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0334301A1 (en) * 1988-03-21 1989-09-27 Chiron Viagene, Inc. Recombinant retroviruses
US5597688A (en) * 1992-03-27 1997-01-28 Ortho Diagnostic Systems Inc. Cell fixative and method of analyzing virally infected cells
DE19709512A1 (en) * 1997-03-08 1998-09-10 Gerd Prof Dr Dr Hobom Recombinant influenza virus
EP1016418A2 (en) * 1994-10-03 2000-07-05 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Composition comprising a recombinant virus expressing an antigen and a recombinant virus expressing an immunostimulatory molecule
US6197531B1 (en) * 1997-01-22 2001-03-06 Center For Blood Research, Inc. Method for determining the immunocompetence of a mammal and therapies related thereto
WO2001072995A2 (en) * 2000-03-28 2001-10-04 University Of Rochester Methods of producing a library and methods of selecting polynucletides

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0334301A1 (en) * 1988-03-21 1989-09-27 Chiron Viagene, Inc. Recombinant retroviruses
US5597688A (en) * 1992-03-27 1997-01-28 Ortho Diagnostic Systems Inc. Cell fixative and method of analyzing virally infected cells
EP1016418A2 (en) * 1994-10-03 2000-07-05 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Composition comprising a recombinant virus expressing an antigen and a recombinant virus expressing an immunostimulatory molecule
US6197531B1 (en) * 1997-01-22 2001-03-06 Center For Blood Research, Inc. Method for determining the immunocompetence of a mammal and therapies related thereto
DE19709512A1 (en) * 1997-03-08 1998-09-10 Gerd Prof Dr Dr Hobom Recombinant influenza virus
WO2001072995A2 (en) * 2000-03-28 2001-10-04 University Of Rochester Methods of producing a library and methods of selecting polynucletides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHAKRABARTI S ET AL: "VACCINIA VIRUS EXPRESSION VECTOR: COEXPRESSION OF BETA-GALACTOSIDASE PROVIDES VISUAL SCREENING OF RECOMBINANT VIRUS PLAQUES", MOLECULAR AND CELLULAR BIOLOGY, WASHINGTON, DC, US, vol. 5, no. 12, December 1985 (1985-12-01), pages 3403 - 3409, XP001017918, ISSN: 0270-7306 *
DEL MAR LORENZO MARIA ET AL: "PCR-based method for the introduction of mutations in genes cloned and expressed in vaccinia virus.", BIOTECHNIQUES, vol. 24, no. 2, February 1998 (1998-02-01), pages 308 - 313, XP001121540, ISSN: 0736-6205 *
DOMINGUEZ J ET AL: "Green fluorescent protein expressed by a recombinant vaccinia virus permits early detection of infected cells by flow cytometry", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 220, no. 1-2, 1 November 1998 (1998-11-01), pages 115 - 121, XP004144108, ISSN: 0022-1759 *
MOSS B: "VACCINIA VIRUS: A TOOL FOR RESEARCH AND VACCINE DEVELOPMENT", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 252, no. 5013, 21 June 1991 (1991-06-21), pages 1662 - 1667, XP000381754, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
WO2002090550A2 (en) 2002-11-14
AU2002307938A1 (en) 2002-11-18
GB0111442D0 (en) 2001-07-04

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