WO2002087769A3 - Verfahren zur parallelen synthese und übertragung von molekülen auf ein substrat - Google Patents

Verfahren zur parallelen synthese und übertragung von molekülen auf ein substrat Download PDF

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Publication number
WO2002087769A3
WO2002087769A3 PCT/EP2002/004670 EP0204670W WO02087769A3 WO 2002087769 A3 WO2002087769 A3 WO 2002087769A3 EP 0204670 W EP0204670 W EP 0204670W WO 02087769 A3 WO02087769 A3 WO 02087769A3
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WO
WIPO (PCT)
Prior art keywords
molecules
matrix
production
libraries
substrate
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PCT/EP2002/004670
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English (en)
French (fr)
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WO2002087769A2 (de
Inventor
Andre Bernard
Stefan Duebel
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Lifebits Ag
Andre Bernard
Stefan Duebel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2001123644 external-priority patent/DE10123644A1/de
Application filed by Lifebits Ag, Andre Bernard, Stefan Duebel filed Critical Lifebits Ag
Priority to DE50210762T priority Critical patent/DE50210762D1/de
Priority to US10/475,801 priority patent/US20040203085A1/en
Priority to EP02724299A priority patent/EP1397216B1/de
Priority to AU2002254994A priority patent/AU2002254994A1/en
Publication of WO2002087769A2 publication Critical patent/WO2002087769A2/de
Publication of WO2002087769A3 publication Critical patent/WO2002087769A3/de

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
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    • B01J2219/00277—Apparatus
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/0061—The surface being organic
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    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
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    • B01J2219/00623—Immobilisation or binding
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Abstract

Die vorliegende Erfindung bezieht sich auf ein Verfahren zur hochparallelen Herstellung komplexer Molekül-Bibliotheken auf einer Substrat-Oberfläche mit hilfe regenerierbarer Matrizen-Stempel. Nach dem Stand der Technik erfordert die Herstellung von Molekül-Bibliotheken auf einem Träger einen hohen apparativen sowie Zeit- und Kostenaufwand. Durch die Verwendung regenerierbarer Matrizen-Stempel kann die Herstellung von Molekül-Bibliotheken, insbesondere von DNA und Protein-Bibliotheken, stark vereinfacht und beschleunigt werden. Der Matrizen-10 Stempel aus einem elastischen Material wie Polydimethylsiloxan trägt auf der Oberfläche ortsgebundene Matrizen-Moleküle, die als Vorlage zur Synthese von Abbild-Molekülen dienen. Die Abbild-Moleküle werden durch ein Kontaktdruck-Verfahren auf die Ziel-Oberfläche übertragen. Die Matrizen-Moleküle bleiben auf dem Stempel für weitere Synthese- und Druckzyklen, die mehrfach wiederholt werden können, zurück. Das Verfahren eignet sich auch zum Kopieren sowie zur Vervielfältigung anderweitig hergestellter Molekül-Arrays.
PCT/EP2002/004670 2001-04-26 2002-04-26 Verfahren zur parallelen synthese und übertragung von molekülen auf ein substrat WO2002087769A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE50210762T DE50210762D1 (de) 2001-04-26 2002-04-26 Verfahren zur parallelen synthese und übertragung von molekülen auf ein substrat
US10/475,801 US20040203085A1 (en) 2001-04-26 2002-04-26 Method for parallel synthesis and transfer of molecules to a substrates
EP02724299A EP1397216B1 (de) 2001-04-26 2002-04-26 Verfahren zur parallelen synthese und übertragung von molekülen auf ein substrat
AU2002254994A AU2002254994A1 (en) 2001-04-26 2002-04-26 Method for parallel synthesis and transfer of molecules to a substrate

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10121574.6 2001-04-26
DE10121574 2001-04-26
DE2001123644 DE10123644A1 (de) 2001-05-04 2001-05-04 Verfahren zum Aufbringen von Proteinen auf einen Träger
DE10123644.1 2001-05-04

Publications (2)

Publication Number Publication Date
WO2002087769A2 WO2002087769A2 (de) 2002-11-07
WO2002087769A3 true WO2002087769A3 (de) 2003-12-31

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FR2921002B1 (fr) * 2007-09-13 2010-11-12 Innopsys Procede de depot simultane d'un ensemble de motifs sur un substrat par un macro timbre
DE102014102434B3 (de) * 2014-02-25 2015-07-30 Karlsruher Institut für Technologie Verfahren, Vorrichtung und Verwendung zum Übertragen von Molekülarrays
US11517873B2 (en) 2019-10-29 2022-12-06 International Business Machines Corporation Controlled chemical synthesis using polymer substrates and nanofluidic separation systems

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DE19543232A1 (de) * 1995-11-07 1997-05-15 Knoell Hans Forschung Ev Herstellung einer Matrix-gebundenen miniaturisierten kombinatorischen Poly- und Oligomerbibliothek
US6096386A (en) * 1996-09-06 2000-08-01 International Business Machines Corporation Method of oriented depositing chemically defined bodies

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19543232A1 (de) * 1995-11-07 1997-05-15 Knoell Hans Forschung Ev Herstellung einer Matrix-gebundenen miniaturisierten kombinatorischen Poly- und Oligomerbibliothek
US6096386A (en) * 1996-09-06 2000-08-01 International Business Machines Corporation Method of oriented depositing chemically defined bodies

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WO2002087769A2 (de) 2002-11-07
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AU2002254994A1 (en) 2002-11-11
EP1397216B1 (de) 2007-08-22
DE50210762D1 (de) 2007-10-04

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