WO2002086150A3 - Method for detecting a reaction by measuring a pm change - Google Patents

Method for detecting a reaction by measuring a pm change Download PDF

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Publication number
WO2002086150A3
WO2002086150A3 PCT/FR2002/001436 FR0201436W WO02086150A3 WO 2002086150 A3 WO2002086150 A3 WO 2002086150A3 FR 0201436 W FR0201436 W FR 0201436W WO 02086150 A3 WO02086150 A3 WO 02086150A3
Authority
WO
WIPO (PCT)
Prior art keywords
substrate
product
measuring
detecting
metal ion
Prior art date
Application number
PCT/FR2002/001436
Other languages
French (fr)
Other versions
WO2002086150A2 (en
Inventor
Jean-Louis Reymond
Gerard Klein
Hong-Khanh Nguyen
Original Assignee
Proteus
Jean-Louis Reymond
Gerard Klein
Hong-Khanh Nguyen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus, Jean-Louis Reymond, Gerard Klein, Hong-Khanh Nguyen filed Critical Proteus
Priority to AU2002302697A priority Critical patent/AU2002302697A1/en
Publication of WO2002086150A2 publication Critical patent/WO2002086150A2/en
Publication of WO2002086150A3 publication Critical patent/WO2002086150A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

The invention concerns a method for detecting the transformation of a substrate (S) into a product (P), characterised in that it consists in measuring the pM modification resulting from the difference of the chelating power of the substrate (S) and of the product (P) relative to a metal ion. Advantageously, the detection is carried out by measuring the modification of the spectral properties of a chemical detector (L), chelating agent for a metal ion, resulting from the metal ion exchanges between the product (P) and the chemical detector (L), when the product (P) has a chelating power higher than that of the substrate (S), or between the substrate (S) and the chemical detector (L), when the substrate (S) has a chelating power higher than that of the product (P).
PCT/FR2002/001436 2001-04-25 2002-04-25 Method for detecting a reaction by measuring a pm change WO2002086150A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002302697A AU2002302697A1 (en) 2001-04-25 2002-04-25 Method for detecting a reaction by measuring a pm change

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0105574A FR2824141B1 (en) 2001-04-25 2001-04-25 METHOD FOR DETECTING TRANSFORMATION OF SUBSTRATE BY MEASURING A CHANGE OF PM, PRODUCTS USED AND DETECTED ACCORDING TO SAID METHOD
FR01/05574 2001-04-25

Publications (2)

Publication Number Publication Date
WO2002086150A2 WO2002086150A2 (en) 2002-10-31
WO2002086150A3 true WO2002086150A3 (en) 2003-01-23

Family

ID=8862681

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/001436 WO2002086150A2 (en) 2001-04-25 2002-04-25 Method for detecting a reaction by measuring a pm change

Country Status (3)

Country Link
AU (1) AU2002302697A1 (en)
FR (1) FR2824141B1 (en)
WO (1) WO2002086150A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077200A (en) * 1987-09-30 1991-12-31 Behringwerke Aktiengesellschaft Azo dye chromogenic substrates
US5252462A (en) * 1988-05-05 1993-10-12 Pharmacia Ab Enzyme activity determinations method characterized by the using of substrates whose fluorescent properties differs those of the converted products

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077200A (en) * 1987-09-30 1991-12-31 Behringwerke Aktiengesellschaft Azo dye chromogenic substrates
US5252462A (en) * 1988-05-05 1993-10-12 Pharmacia Ab Enzyme activity determinations method characterized by the using of substrates whose fluorescent properties differs those of the converted products

Also Published As

Publication number Publication date
AU2002302697A1 (en) 2002-11-05
FR2824141A1 (en) 2002-10-31
FR2824141B1 (en) 2005-12-23
WO2002086150A2 (en) 2002-10-31

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