WO2002084271A3 - Methode et capteurs de detection et d'analyse luminescente et optoelectronique de polynucleotides - Google Patents

Methode et capteurs de detection et d'analyse luminescente et optoelectronique de polynucleotides Download PDF

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Publication number
WO2002084271A3
WO2002084271A3 PCT/US2002/012176 US0212176W WO02084271A3 WO 2002084271 A3 WO2002084271 A3 WO 2002084271A3 US 0212176 W US0212176 W US 0212176W WO 02084271 A3 WO02084271 A3 WO 02084271A3
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WO
WIPO (PCT)
Prior art keywords
methods
polynucleotide
sensor
sensor polynucleotide
polynucleotides
Prior art date
Application number
PCT/US2002/012176
Other languages
English (en)
Other versions
WO2002084271A2 (fr
Inventor
Daniel E Morse
Galen D Stucky
Jennifer N Cha
Original Assignee
Univ California
Daniel E Morse
Galen D Stucky
Jennifer N Cha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Daniel E Morse, Galen D Stucky, Jennifer N Cha filed Critical Univ California
Publication of WO2002084271A2 publication Critical patent/WO2002084271A2/fr
Publication of WO2002084271A3 publication Critical patent/WO2002084271A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)

Abstract

L'invention concerne des méthodes, des compositions et des articles d'analyse d'un échantillon à la recherche d'un polynucléotide cible. Un échantillon susceptible de contenir le polynucléotide cible est mis en contact avec un polynucléotide capteur monocaténaire complémentaire du polynucléotide cible et un agent permettant au polynucléotide capteur lui-même, lorsqu'il est présent sous forme monocaténaire, de devenir fluorescent avec l'excitation. Le polynucléotide capteur est éventuellement conjugué à un substrat, qui peut être un dispositif de détection optoélectronique, et peut être micro ou nano adressable. Un chromophore peut être utilisé pour absorber l'énergie du polynucléotide capteur excité et émettre de la lumière. Les méthodes de l'invention peuvent être utilisées sous forme multiplexe. L'invention concerne également des dispositifs de détection incorporant le polynucléotide capteur et éventuellement le chromophore. L'invention concerne enfin des kits comprenant des réactifs permettant de mettre en oeuvre lesdites méthodes.
PCT/US2002/012176 2001-04-16 2002-04-16 Methode et capteurs de detection et d'analyse luminescente et optoelectronique de polynucleotides WO2002084271A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83657901A 2001-04-16 2001-04-16
US09/836,579 2001-04-16

Publications (2)

Publication Number Publication Date
WO2002084271A2 WO2002084271A2 (fr) 2002-10-24
WO2002084271A3 true WO2002084271A3 (fr) 2002-12-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/012176 WO2002084271A2 (fr) 2001-04-16 2002-04-16 Methode et capteurs de detection et d'analyse luminescente et optoelectronique de polynucleotides

Country Status (1)

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WO (1) WO2002084271A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7914984B2 (en) 2003-09-17 2011-03-29 The Regents Of The University Of California Conformationally flexible cationic conjugated polymers

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9371559B2 (en) 2002-06-20 2016-06-21 The Regents Of The University Of California Compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US10001475B2 (en) 2002-06-20 2018-06-19 The Regents Of The University Of California Light harvesting multichromophore compositions and methods of using the same
WO2004092324A2 (fr) 2002-08-26 2004-10-28 The Regents Of The University Of California Procedes et compositions de detection et d'analyse de polynucleotides au moyen de multichromophores collecteurs de lumiere
EP1549772B1 (fr) * 2002-08-26 2013-01-02 The Regents of The University of California Procedes et compositions de detection et d'analyse de polynucleotides au moyen de multichromophores collecteurs de lumiere
AU2004215006A1 (en) 2003-02-13 2004-09-10 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotide-binding protein interactions using light harvesting multichromophores
EP1838752B1 (fr) 2005-01-10 2017-10-04 The Regents of The University of California Polymeres conjugues cationiques appropries pour la detection de polynucleotides specifiques de brins dans des essais a l'etat solide et homogenes
WO2006074482A2 (fr) 2005-01-10 2006-07-13 The Regents Of The University Of California Procedes et articles pour la detection de polynucleotides specifiques de brin a multichromophores cationiques
US7666594B2 (en) 2005-01-31 2010-02-23 The Regents Of The University Of California Methods for assaying a sample for an aggregant

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4840892A (en) * 1984-05-15 1989-06-20 Smithkline Beckman Corporation Polynucleotide hybridization probes
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
US5998597A (en) * 1996-10-30 1999-12-07 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US6015880A (en) * 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6110339A (en) * 1995-06-08 2000-08-29 Visible Genetics Inc. Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same
US6258606B1 (en) * 1996-07-09 2001-07-10 Nanogen, Inc. Multiplexed active biologic array
US6262241B1 (en) * 1990-08-13 2001-07-17 Isis Pharmaceuticals, Inc. Compound for detecting and modulating RNA activity and gene expression
US6274323B1 (en) * 1999-05-07 2001-08-14 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4840892A (en) * 1984-05-15 1989-06-20 Smithkline Beckman Corporation Polynucleotide hybridization probes
US6262241B1 (en) * 1990-08-13 2001-07-17 Isis Pharmaceuticals, Inc. Compound for detecting and modulating RNA activity and gene expression
US6015880A (en) * 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US6110339A (en) * 1995-06-08 2000-08-29 Visible Genetics Inc. Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6258606B1 (en) * 1996-07-09 2001-07-10 Nanogen, Inc. Multiplexed active biologic array
US5998597A (en) * 1996-10-30 1999-12-07 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US6274323B1 (en) * 1999-05-07 2001-08-14 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MORRISON L.E. ET AL.: "Solution-phase detection of polynucleotides using interacting fluorescent labels and competitive hybridization", ANALYTICAL BIOCHEMISTRY, vol. 183, June 1989 (1989-06-01), pages 231 - 244, XP002113097 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7914984B2 (en) 2003-09-17 2011-03-29 The Regents Of The University Of California Conformationally flexible cationic conjugated polymers

Also Published As

Publication number Publication date
WO2002084271A2 (fr) 2002-10-24

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