PROCESS FOR THE PRODUCTION OF AMORPHOUS ATORVASTATIN CALCIUM
FIELD OF THE INVENTION
The present invention relates to a novel process for the production of atorvastatin calcium. Particularly, the present invention relates to a novel process for the production of amorphous atorvastatin calcium. More particularly, the present invention relates to a novel process for the production of amorphous atorvastatin calcium from a diol protected tert-butyl ester (a) BACKGROUND OF THE INVENTION
Atorvastatin is chemically [R-(R*,R*)]-2-(4-FLUOROPHENYL)-β,δ- DIHYDROXY-5-(l-METHYLETHYL)-3-PHENYL-4-[(PHENYLAMINO) CARBONYL]-lH-PYRROLE-l-HEPTANOIC ACID. Atorvastatin calcium, a synthetic HMG-CoA reductase inhibitor, is used for the treatment of hyperlipidemia and hypercholesterolemia, both of which are risk factors for arteriosclerosis and coronary heart disease. Open dihydroxy carboxylic acid, lactone and various salt forms of atorvastatin have been synthesized.
According to the disclosure contained in the United States Patent 5,273,995, describes that R-form of the ring opened acid form has surprising inhibition of the biosynthesis of cholesterol. Atorvastatin in its calcium salt form, i.e. [R-(R*,R*)]-2-(4-fluorophenyl)-β,δ-dihydroxy-5-(l-methylethyl)-3-phenyl-4- [(phenylamino)carbonyl]-lH-pyrrole-l-heρtanoic acid calcium salt (2:1) having formula 1:
is more suited to formulations and has been recommended as a drug.
United states patents 5,003,080; 5,097,045; 5,103,024; 5,124,482; 5,149,837;
5,155,251; 5,216,174; 5,245,047; 5,248,793; 5,273,995; 5,280,126; 5,298,627; 5,342,952;
5,385,929; 5,397,792; European Patent 409,281; and PCT publication No.
8,907,598 describe various processes and key intermediates for preparing atorvastatin.
Atorvastatin is, preferably, prepared as its calcium salt, i.e. [R-(R*,R*)]-2-
(4-fluorophenyl)-β,δ-dihydroxy-5-(l-methylethyl)-3-phenyl-4- [(phenylamino)carbonyl]-lH-ρyrrole-l-heptanoic acid calcium salt (2 : 1) since the calcium salt is desirable and it, enables easy formulation of atorvastatin for example, tablets, capsules, lozenges, powder and the Uke for oral administration.
One of the major drawbacks of the prior art processes referred to above is that none of these processes consistently produce amorphous atorvastatin calcium but generally gives a mixture of its crystalUne and amorphous forms with unsuitable filtration and drying characteristics, rendering them unsuitable for large-scale production.
PCT appUcation, WO 97/03958 and WO 97/03959 disclose novel crystalline forms of atorvastatin calcium designated as Form I, Form II, Form III and Form IV and method for their preparation which provide more favorable filtration and drying characteristics. PCT application, WO 97/03960 and US Patent 6087511 describe the procedures for converting the crystaUine form of atorvastatin calcium to the amorphous form. The process disclosed therein involve dissolving form I atorvastatin calcium in a non hydroxylic solvent Uke tetrahydrofuran or a mixture of tetrahydrofuran and toluene. None of these processes disclosed therein is suitable for large scale production as solvent has to be removed at high temperature about 85 - 90°C and under high vacuum (5 - 10 mm of mercury) and the product thus obtained is in the form of a brittle glassy foam which has to be broken into a free flowing powder. The process disclosed therein also takes very long time for the removal of solvents PCT application WO 00/71116 describes the procedure for converting the crystalline form-I by dissolving it in a non-hydroxylic solvent like tetrahydrofuran and precipitating amorphous atorvastatin calcium by the
addition of nonpolar hydrocarbon solvents like, n-hexane, cyclohexane or n- heptane. The method disclosed in this PCT application is not suitable for large scale production of amorphous atorvastatin calcium as the process requires very large amount of non polar hydrocarbon solvents, i.e about 20 - 40 times as that of crystalUne atorvastatin calcium and the mixture of tetrahydrofuran and non- polar hydrocarbon solvent obtained from the mother liquor would not be of any use for further recycUng of the solvents. Secondly the solvents used therein have very low flash points (tetrahydrofuran -^ -17°C, n-hexane -^ -23°C, n- heptane -> -1°C) making the process very unsafe on the commercial scale. Objects of the invention
It is therefore, an object of the present invention to provide a process for the preparation of amorphous atorvastatin calcium which avoids aU the disadvantages of the prior art
It is a further object of the present invention to provide a process for the preparation of amorphous atorvastatin calcium consistently and which avoids production of a mixture of amorphous and crystaUine forms.
It is yet another object of the present invention to provide a process for the preparation of amorphous atorvastatin calcium, which is economical and capable of being practiced on a commercial scale. It is a further object of the present invention to provide a process for the conversion of crystalUne atorvastatin calcium in to its amorphous form, which is economical and is capable of being practiced on a commercial scale. Summary of the invention
The above and other objects of the present invention are achieved by a novel process for the preparation of amorphous form of atorvastatin calcium directly from the intermediate (4R-cis)-l,l-dimethylethyl-6-[2-[2-(4- fluorophenyl)-5-(l-methylethyl)-3-phenyl-4-[(phenylamino)-carbonyl]-lH- pyrrol-l-yl]-ethyl]-2,2-dimethyl-l,3-dioxane-4-acetate, diol protected tert-butyl ester (a) which is an intermediate in the synthesis of atorvastatin lactone, a starting material from which different polymorphs of atorvastatin calcium such as Form I and Form IV may be prepared. Similarly, preparation of Form II, Form III, and amorphous form by interconversion has been reported in the prior
arts. However, to the applicants' knowledge, not a single process is available to prepare amorphous form of atorvastatin calcium directly from the above mentioned intermediate (a), without employing corresponding lactone compound. Accordingly, the present invention discloses a novel process for the preparation of amorphous atorvastatin calcium from the above mentioned intermediate (a) on commercial scale, while in earlier prior arts, crystaUine form - I of atorvastatin calcium is employed for the preparation of amorphous atorvastatin calcium. The present invention also discloses a novel process of converting form - I of atorvastatin calcium into amorphous form, which is suitable for converting all the crystalline forms of atorvastatin calcium in to amorphous form on commercial scale. Detailed description
The process of the present invention eUminates the problems of prior arts. The present invention also discloses for the first time a process of manufacturing amorphous atorvastatin calcium directly from diol protected tertbutyl ester (a). The crude stage may contain some amount of calcium hydroxide which is easily removed completely in the subsequent purification stage. At both the stages of preparation, atorvastatin calcium prepared by the process of the present invention was found to be amorphous as revealed by X-ray powder diffraction data (figure -1 & 2). The present invention does not make use of any crystalline form to get amorphous atorvastatin calcium like other prior arts. However conversion of form- 1 of atorvastatin calcium in to amorphous atorvastatin calcium disclosed herein is an additional procedure within the scope of the present invention to exploit on commercial scale and does not in any way deviate from the basic purpose of this invention of getting amorphous atorvastatin calcium without employing any crystaUine form. In the present invention, purification stage of atorvastatin calcium is conveniently used to convert crystalline forms of atorvastatin calcium into amorphous atorvastatin calcium. The present invention does not employ corresponding lactone compound to convert it into atorvastatin calcium like other prior arts. The present invention makes the use of relatively safer solvent i.e. methanol with higher flash point -> +11°C.
Accordingly, the present invention provides a process for the preparation of atorvastatin calcium in amorphous form which comprises treating diol protected tert-butyl ester of following structure (a) below
(a) with a methanolic solution in the presence of a dilute acid and subsequently treating the reaction mixture with dilute hydroxide solution and then with dilute calcium chloride solutions to obtain a white slurry of the material in the solution, removing the material from the solution to obtain crude amorphous atorvastatin calcium and purifying said crude amorphous atorvastatin calcium to obtain pure amorphous atorvastatin calcium. Preferably, the crude material is removed from the solution centrifuging the solution and drying the product so obtained at 50 - 60 °C for several hours till water content 4 - 6% is achieved. The crude amorphous atorvastatin calcium so obtained contains some amount of calcium hydroxide formed during the reaction of calcium chloride with free sodium hydroxide present in the reaction mixture. The crude material is purified by dissolving it in methanol (10% w/v) giving opalescence due to calcium hydroxide. Preferably, the crude material is dissolved in methanol at 30 - 35 °C (10% w/v) and treated with activated carbon and filtered through hyflow bed over nutsch filter. The clear solution thus obtained is again filtered through 5-micron candle filter to get absolutely clear and transparent methanolic solution of atorvastatin calcium. This solution is added slowly to fine filtered D.M. Water at 50 - 55°C (the amount of water is taken twice as that of methanolic solution v/v), resulting in the free flowing precipitation of pure amorphous atorvastatin calcium with normal agitation (70 rpm) which is cooled to 10 - 15°C and then centrifuged and dried at 50 - 60°C for several hours tiU water content 4 - 6% is achieved.
Preferably, the aqueous acid is selected from hydrochloric acid , sulphuric acid , and formic acid; aqueous hydrochloric acid is being most preferred. The aqueous hydroxide solution is selected from sodium hydroxide, potassium hydroxide, and lithium hydroxide. In a most preferred embodiment, aqueous sodium hydroxide is employed.
In another preferred embodiment, the crude salt as well as the purified products are isolated by filtration and then dried.
In another preferred embodiment, the diol protected tert-butyl ester of the structure (a) is treated with 20 - 40 times w/v methanol. More preferably, the amount of methanol employed is 28 times w/v.
The HC1 employed is preferably 2 -6% aqueous w/v, more preferably 4% w/v in a molar ratio of 1.5 - 4, preferably 2.0. The reaction temperature is preferably maintained at a range of from 20-40°C preferably, 30 -35°C for 8 - 20 hours preferably for 15 hours. In another preferred embodiment, the dilute aqueous sodium hydroxide solution employed ranges from 5 - 20% w/v, preferably 10% w/v in molar ratio of 1 - 1.5, more preferably, 1.35, (after calculating the amount of sodium hydroxide required for neutraUzation of hydrochloric acid present in the reaction mixture). The reaction mass is stirred for 2 - 6 hours, preferably, for 5 hours
The pH of the reaction mixture is preferably maintained 7.0 - 9.5, more preferably 8.5 by addition of 4% w/v aqueous hydrochloric acid. In preferred embodiment, pH is always maintained at a level > 7.0.
The aqueous calcium chloride solution is preferably employed in the range of 2 - 6% w/v preferably 4 - 5 % in the molar ratio of 1 - 2, more preferably 1.65. The reaction temperature is maintained at a temperature in the range of 50 - 55°C for 15 - 60 minutes preferably for 60 minutes.
The precipitated material is preferably cooled to 25 - 40°C, more preferably to 30 - 35°C, which is further cooled to 0 - 20°C preferably, to 10 -
15°C. The stirring is continued for 30 - 120 minutes preferably, for 60 minutes at
10 - 15°C The material is centrifuged easily and is preferably washed with D.M. Water to remove excess calcium chloride.
The present invention describes the method of converting diol protected tert-butyl ester (a) of atorvastatin directly into crude amorphous atorvastatin calcium which contains some amount of calcium hydroxide which is removed in subsequent purification step. The whole process consists of following key operations
1) Treating of diol protected tert-butyl ester (a, scheme - 1) in methanol 20 - 40 w/v times methanol, as that of diol protected tert-butyl ester, preferably 28 times with 2 -6% aqueous w/v hydrochloric acid preferably 4% w/v hydrochloric acid solution in a molar ratio of 1.5 - 4 preferably 2.0 at the temperature range of 20-40°C preferably at 30 - 35°C for 8 - 20 hours preferably for 15 hours. HPLC analysis of reaction mixture after 15 hours shows the presence of unreacted diol protected tert-butyl ester (a) (0.56%), as in scheme - 1. HPLC analysis also shows formation of 4 intermediates (b,c,d and e) as shown in scheme - 1 with distribution pattern as follows. This is an illustrative pattern. The percentage and distribution may vary depending on the reaction conditions, (i) atorvastatin diol tert-butyl ester (b) - 72.00% (ii) atorvastatin diol methyl ester (c) - 21.16%
(in) atorvastatin lactone (d) - 2.52%
(iv) atorvastatin diol acid (e) - 0.96%
2) The above solution is treated with dilute aqueous sodium hydroxide solution ranging from 5 - 20% w/v preferably 10% w/v in molar ratio of 1 - 1.5 preferably 1.35 (after calculating the amount of sodium hydroxide required for neutralization of hydrochloric acid present in the reaction mixture). The reaction mass is stirred for 2 - 6 hours preferably for 5 hours when HPLC analysis shows the complete conversion of aU the intermediates (b,c,d,and e) as mentioned in scheme - 1 into a single product, atorvastatin sodium (f) in the solution.
3) pH of the reaction mixture is adjusted between 7.0 - 9.5, preferably 8.5 by addition of 4% w/v aqueous hydrochloric acid, pH lower than 7.0 results in
the formation of lactone compound (d) in the final product, this also results in the decrease of the calcium content below the required amount i.e. 3.50 % w/w on dry basis.
4) The volume of the reaction mixture is then reduced to approximately 50% by distiUation under reduced pressure below 60°C
5) The volume of the reaction mixture is measured and the content of methanol and water are determined v/v.
6) The volume of the reaction mixture is then adjusted so that it contains 5 -15 times, preferably 10 times methanol and 5 - 10 times, preferably 7 times water with respect to diol protected tert-butyl ester (a) initially taken.
7) The reaction mixture is then washed with 5 - 15 times preferably 10 times as that of diol protected tert-butyl ester (a) taken for reaction with organic solvents insoluble in water such as toluene, xylene, dnsopropyl ether, diethyl ether, dichloromethane preferably diisopropyl ether to remove starting material i.e. unreacted diol protected tert-butyl ester (a) from the reaction mixture.
8) Aqueous methanolic layer after extraction with diisopropyl ether is charged into another S.S.Reactor, finaUy pH is checked and if necessary, is adjusted to 8.5, the reaction mixture is heated to 40 - 60°C preferably to 50 - 55°C.
9) Aqueous calcium chloride solution in the range of 2 - 6% w/v preferably 4 - 5% in the molar ratio of 1 - 2 preferably 1.65 is added during 30 - 90 minutes preferably 60 minutes at 50 - 55°C with the smooth and uniform precipitation of atorvastatin calcium. If mode of addition is reversed, a sticky material is obtained under similar condition of operations.
10) The precipitated material is stirred at 50 - 55°C for 15 - 60 minutes preferably for 60 minutes.
11) The precipitated material is cooled to 25 - 40°C preferably to 30 - 35°C, which is further cooled to 0 - 20°C preferably to 10 - 15°C. 12) The stirring is continued for 30 - 120 minutes preferably for 60 minutes at 10 - 15°C
13) The material is centrifuged easily and is washed with D.M. Water to remove excess calcium chloride.
14) The wet cake of crude amorphous atorvastatin calcium which is 3 - 4 times as that of dried material is dried in a vacuum dryer at 40 - 70°C preferably at 50 - 60°C for several hours preferably for 8 - 10 hours tiU the water content in the range of 2 - 8% preferably 4 - 6% is achieved.
15) The dried material was found to contain traces of calcium hydroxide which is formed during the calcium chloride addition (excess sodium hydroxide in the reaction mixture reacts with calcium chloride to produce calcium hydroxide) which is insoluble in water and hence can not be removed even washing with plenty of D.M.Water. The next operations afterwards are being carried out to remove calcium hydroxide present in the crude amorphous atorvastatin calcium.
16) X-Ray powder diffraction study shows crude atorvastatin calcium to be amorphous (Fig. - 1).
17) Crude amorphous atorvastatin calcium is dissolved in methanol at 30 - 60°C preferably at 30 - 35°C in 5 - 15 times in volume preferably 7 times in volume as that of crude atorvastatin calcium and treated with activated carbon 2 - 10% w/w preferably 5% w/w at 30 - 35°C for 15 - 60 minutes preferably for 30 minutes.
18) Methanolic solution of atorvastatin calcium is then filtered through hyflow bed contained in a nutsche filter under vacuum and the bed is washed with fresh methanol.
19) The solution is finally filtered through 5 micron candle filter to get absolutely clear and transparent solution, free from any suspended particles. The filtration stages (18) and (19) completely remove calcium hydroxide present in the crude product.
20) The final volume of methanolic atorvastatin calcium is adjusted to 8 - 12 times in volume preferably 10 times in volume as that of crude atorvastatin calcium by addition of fine filtered methanol.
21) In another S.S.Reactor D.M.Water is charged 1 - 3 times preferably 2 times in volume as that of methanolic solution of atorvastatin calcium through 5 micron candle filter which is heated to 40 - 60°C preferably to 50- 55°C.
22) Methanolic solution of atorvastatin calcium is added slowly during 15 - 60 minutes preferably in 30 minutes at 50 - 55°C with free flowing and uniform formation of amorphous atorvastatin calcium in the reactor.
23) The precipitated material is stirred at 50 - 55°C for 10 - 40 minutes preferably for 20 - 30 minutes.
24) The precipitated material is cooled to 25 - 40°C preferably to 30 - 35°C which is further cooled to 0 - 20°C preferably to 10 - 15°C.
25) The stirring is continued for 30 - 120 minutes preferably for 60 minutes at 10 - 15°C
26) The material is centrifuged easily and is washed with plenty of D.M.Water.
27) The wet cake of pure amorphous atorvastatin calcium which is 3 - 4 times in weight as that of dried material is dried in a vacuum dryer at 40 - 80°C preferably at 50 - 60°C for several hours preferably for 8 - 10 hours till the water content in the range of 2 - 8% preferably 4 - 6% is achieved. 28) X-Ray powder diffraction study shows pure atorvastatin calcium to be amorphous and the product is free from calcium hydroxide. Major advantages of the present invention compared to the prior art processes are:-
1. Direct conversion of diol protected tert-butyl ester (a, scheme - 1) into amorphous atorvastatin calcium in a hydroxyUc solvents without preparation and isolation of lactone compound. 2. Purification stage removes calcium hydroxide present in atorvastatin calcium completely.
3. Crystalline forms of atorvastatin calcium are conveniently converted into amorphous atorvastatin calcium using purification procedures disclosed herein exemplified by conversion of form - I of atorvastatin calcium in to amorphous atorvastatin calcium.
4. The process disclosed herein requires no high agitation or vigorous stirring like prior arts.
5. Avoiding the need to remove the solvent at higher temperature and under high vacuum.
6. Avoiding the need of using the solvents with lower flash points Uke tetrahydrofuran, n-hexane and n-heptane 7. Avoiding the need of using an uneconomical solvent like tetrahydrofuran.
8. Avoiding the need of recycling and separating a mixture of solvents obtained from mother Uquor.
9. The process disclosed herein employs only one solvent in the manufacturing process i.e methanol that can be easily recovered, and recycled. The process disclosed herein produces amorphous product consistently on commercial scale with allowable Umits of residual solvent (methanol ■ 500 - 1000 ppm)
10. The process disclosed herein is carried out by using relatively safer solvent (methanol) with flash point of +11°C. 11. The process disclosed herein gives amorphous form directly without interconversion of any crystalUne form into amorphous form.
The present invention wiU now be described in greater detatf with reference to the accompanying drawings and examples in which
Figure 1 depicts X-ray powder diffractogram of amorphous atorvastatin calcium crude. The horizontal axis presents 2Θ and the vertical axis corresponds to peak intensity. Figure 2 shows X-ray powder diffractogram of amorphous atorvastatin calcium pure. The horizontal axis presents 2Θ and the vertical axis corresponds to peak intensity.
Figure 3 shows X-ray powder diffractogram of form - I of atorvastatin calcium. The horizontal axis presents 2Θ and the vertical axis corresponds to peak intensity. Figure 4 illustratesX-ray powder diffractogram of amorphous atorvastatin calcium prepared by converting form - I of atorvastatin
calcium. The horizontal axis presents 2Θ and the vertical axis corresponds to peak intensity.
Scheme 1 describes Schematic representation and distribution of the intermediates formed during the treatment of methanolic solution of diol protected tert-butyl ester (a) with dilute hydrochloric acid and subsequent conversion of aU the intermediates into a single product atorvastatin sodium and then to atorvastatin calcium.
The present invention will now be illustrated by the following examples, which are not intended to Umit the effective scope of the claims. Consequently, any variations of the invention described above are not to be regarded as departure from the spirit and scope of the invention as claimed. The present invention has been described in terms of its specific embodiments and various modifications and equivalents will be apparent to those skiUed in the art and are intended to be included within the scope of present invention.
Example 1 [R-(R*,R*)]-2-(4-fluorophenyl)-β/δ-dihydroxy-5-(l-methylethyl)-3-phenyl-4- [(phenylamino) carbonyl]-lH pyrrole-1-heptanoic acid hemicalcium salt (Crude amorphous atorvastatin calcium). 20 Kg. of diol protected tert-butyl ester (a) is suspended in 560 It. methanol in GLR and treated with 55 It. of 4% aqueous w/v hydrochloric acid for 15 hours at 30 - 35°C then a solution of 4 Kg. sodium hydroxide in 40 It. D.M.Water is added in 1 hour and stirring is continued for 5 hours. The pH of reaction mixture is adjusted to 8.5 by addition of 1 It. of 4% w/v aqueous hydrochloric acid. The volume of the reaction mixture is reduced to approximately 50% by distiUing below 60°C under vacuum (distiUed volume 270 - 275 It), the analysis of the contents left behind in the reactor shows water content to be 83 It. and methanol content to be 178 It. Then 23 It. methanol and 57 It. water are added to the reaction mixture, reaction mixture is washed with 200 It. of diisopropylether. Aqueous methanolic solution containing atorvastatin sodium is charged into another S.S.Reactor and finaUy pH is checked and if necessary adjusted to 8.5 and the contents are heated to 50 - 55°C to which a
aqueous solution of 2.8 Kg. calcium chloride in 60 It. water is added in 1 hour at 50 - 55°C with the precipitation of atorvastatin calcium, the precipitated mass is stirred for 1 hr at 50 - 55°C which is cooled to 30 - 35°C (within 60 minutes), then cooled to 10 - 15°C (within 60 minutes), the precipitated material is further stirred for 1 hr at 10 - 15°C . The solid material is centrifuged and washed with D.M.Water. (Wet weight = 55 - 60 Kg.) The material is dried at 50 - 60°C for 8 hours till water content 4 - 6% is achieved giving 17.50 Kg. crude amorphous atorvastatin calcium. X-ray powder diffraction data confirmed the amorphous nature of the ρroduct(Fig-l). Example 2
The example disclosed herein is a purification stage in which calcium hydroxide in atorvastatin calcium is being removed.
17.0 Kg. crude amorphous atorvastatin calcium so obtained in example 1 is dissolved in 120 Lt. methanol and 0.85 Kg. activated carbon is added and stirred at 30 - 35°C for 30 minutes. Methanolic solution of atorvastatin calcium is filtered through hyflow bed over nutsche filter and washed with 20 lt methanol. Filtered methanolic solution is passed through 5 micron candle filter, and the volume of this solution is adjusted to 170 lt. by addition of fresh fine filtered methanol. In another S.S.Reactor 340 lt. D.M.Water is taken through 5 micron candle filter and heated to 50 - 55°C, filtered methanolic solution of atorvastatin calcium is added in 30 minutes at 50 - 55°C in the reactor containing water with precipitation of pure atorvastatin calcium, which is stirred for another 20 minutes, at 50 - 55°C, the contents in reactor are cooled to 30 - 35°C (within 90 minutes) then cooled to 10 - 15°C (within 2 hours), the precipitated material is further stirred at 10 - 15°C for 1 hour. Pure amorphous atorvastatin calcium is centrifuged and washed with 40 lt D.M.Water. (Wet weight = 45 - 50 Kg.) The material is dried at 50 - 60°C for 8 hours till water content 4 - 6% is achieved to give dry weight of 15 Kg. of pure amorphous atorvastatin calcium. X-ray powder diffraction data confirmed the amorphous nature of the product and the material thus obtained is free from calcium hydroxide.
Example 3
The example disclosed herein is a convenient procedure to convert crystalline forms of atorvastatin calcium in to amorphous atorvastatin calcium, exemplified here by converting form - I of atorvastatin calcium in to amorphous form. 20 gm atorvastatin calcium form - I (figure 3) is dissolved in 160 ml methanol at 45 - 50°C and then is treated with 2 gm activated carbon for 15 minutes at 45 - 50°C. the solution is cooled to 25 -35°C and is filtered through hyflow bed with 2 x 20 ml methanol wash. The volume of filtrate is adjusted to 200 ml by addition of fresh methanol. FinaUy filtrate is passed through 5 micron filter pad to get absolutely clear and transparent solution of atorvastatin calcium in methanol. The methanolic solution of atorvastatin calcium is added to 400 ml of fine filtered D.M.Water at 50 - 55°C in 30 minutes, with stirring, resulting in the precipitation of atorvastatin calcium, stirring is continued for another 30 minutes at 50 - 55°C. The precipitated material is cooled to 30 - 35°C then to 10 - 15°C stirring is continued for 1 hr at 10 -15°C The material is filtered and washed with 2 x 50 ml of D.M.Water, dried at 50 -60°C for 8 hours till the water content 4 - 6 % is achieved, giving 18.0 gm dried atorvastatin calcium. X-ray powder diffraction data suggests the material to be amorphous (figure 4)