WO2002082078A3 - Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes - Google Patents

Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes Download PDF

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Publication number
WO2002082078A3
WO2002082078A3 PCT/EP2002/003890 EP0203890W WO02082078A3 WO 2002082078 A3 WO2002082078 A3 WO 2002082078A3 EP 0203890 W EP0203890 W EP 0203890W WO 02082078 A3 WO02082078 A3 WO 02082078A3
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WO
WIPO (PCT)
Prior art keywords
analytes
utilized
molecules
affinity
quantifying
Prior art date
Application number
PCT/EP2002/003890
Other languages
French (fr)
Other versions
WO2002082078A2 (en
Inventor
Reinhard Bredehorst
Rainer Hintsche
Anton Heuberger
Original Assignee
Fraunhofer Ges Forschung
Reinhard Bredehorst
Rainer Hintsche
Anton Heuberger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Ges Forschung, Reinhard Bredehorst, Rainer Hintsche, Anton Heuberger filed Critical Fraunhofer Ges Forschung
Priority to EP02724275A priority Critical patent/EP1448991A2/en
Publication of WO2002082078A2 publication Critical patent/WO2002082078A2/en
Publication of WO2002082078A3 publication Critical patent/WO2002082078A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/586—Liposomes, microcapsules or cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Analytes bound to immobilized capture molecules by molecular biological or immunochemical reactions are detected or quantified with affinity liposomes containing encapsulated enzyme activators and surface-attached affinity components capable of specific binding to captured analytes or derivatives thereof in a structure restricted manner. Enzyme activators encapsulated in analyte-bound affinity liposomes are released by temperature- or detergent-mediated mechanisms and utilized for activation of solid phase-immobilized inactive enzyme molecules which in turn are utilized to generate colorimetric, fluorescent, chemiluminescent, or bioluminescent reporter molecules the quantity of which is a proportiona measure of the amount of analyte in the specimen. For amplified assay procedures polymeric carrier molecules capable of binding multiple affinity liposomes and/or preformed complexes of affinity liposomes are utilized.
PCT/EP2002/003890 2001-04-09 2002-04-08 Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes WO2002082078A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02724275A EP1448991A2 (en) 2001-04-09 2002-04-08 Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens, antibodies and other analytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28208801P 2001-04-09 2001-04-09
US60/282,088 2001-04-09

Publications (2)

Publication Number Publication Date
WO2002082078A2 WO2002082078A2 (en) 2002-10-17
WO2002082078A3 true WO2002082078A3 (en) 2004-06-10

Family

ID=23080057

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/003890 WO2002082078A2 (en) 2001-04-09 2002-04-08 Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes

Country Status (2)

Country Link
EP (1) EP1448991A2 (en)
WO (1) WO2002082078A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602004011198T2 (en) * 2003-03-25 2008-12-24 Biacore Ab IMMOBILIZATION PROCESS AND KIT THEREFOR
US8741577B2 (en) * 2003-04-07 2014-06-03 Bio-Rad Laboratories Inc. Surface immobilised multilayer structure of vesicles
EP1611256B1 (en) * 2003-04-07 2016-03-16 Bio-Rad Laboratories, Inc. Surface immobilised multilayer structure of vesicles
DE602004030623D1 (en) * 2003-09-02 2011-01-27 Systagenix Wound Man Ip Co Bv Signal amplification using synthetic zymogen
FR2918755B1 (en) * 2007-07-09 2009-10-30 Commissariat Energie Atomique METHOD FOR DETECTING AND QUANTIFYING SOLID - SUPPORTED ANALYTES WITH FLUORESCENT MOLECULES ENCAPSULATED IN IMMUNOLIPOSOMES.
BRPI0822092A8 (en) * 2007-12-20 2016-03-22 Koninklijke Philips Electronics Nv MICROELECTRONIC SENSOR DEVICE FOR THE EXAMINATION OF TARGET PARTICLES, METHOD FOR THE EXAMINATION OF TARGET PARTICLES, AND, USE OF THE MICROELECTRONIC SENSOR DEVICE
DE102008027038A1 (en) 2008-06-06 2009-12-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method for detecting chemical or biological species and electrode arrangement therefor
ES2568252T3 (en) 2008-07-11 2016-04-28 Universal Biosensors, Pty. Ltd. Enhanced Immunoassay Sensor
US8383337B2 (en) * 2008-07-18 2013-02-26 General Electric Company Methods using metal oxide particles for analyte detection
CN103645185A (en) * 2013-12-17 2014-03-19 武汉大学 Method for visually detecting alkaline phosphatase
EP3252082A1 (en) * 2016-05-31 2017-12-06 Galderma S.A. Method for deacetylation of biopolymers
SI3397651T1 (en) * 2015-12-29 2020-11-30 Galderma S.A. Carbohydrate crosslinker
PT3623390T (en) * 2016-05-31 2023-10-27 Galderma Sa Carbohydrate crosslinker
EP4069753A1 (en) 2019-12-02 2022-10-12 Galderma Holding SA High molecular weight esthetic compositions
CN111579613B (en) * 2020-05-12 2022-07-08 台州学院 Photoelectric chemical sensing-based fatty acid binding protein detection method
EP4200341A1 (en) * 2020-08-19 2023-06-28 The Regents of the University of California Diagnostic methods and compositions
CN116948169A (en) * 2023-06-14 2023-10-27 华南师范大学 Method for rapidly synthesizing self-enhanced electrochemiluminescence probe in water phase based on polylysine and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4939098A (en) * 1985-05-31 1990-07-03 Hitachi, Ltd. Immunoassay and measurement kit used therefor
WO1999010743A1 (en) * 1997-08-29 1999-03-04 Regents Of The University Of California Sol-gel matrices for direct colorimetric detection of analytes
EP0926497A2 (en) * 1997-12-26 1999-06-30 Hogy Medical Co., Ltd. Colorimetric sensor employing polydiacetylene membrane
WO1999060399A1 (en) * 1998-05-21 1999-11-25 Cornell Research Foundation, Inc. Liposome-enhanced test device and method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4939098A (en) * 1985-05-31 1990-07-03 Hitachi, Ltd. Immunoassay and measurement kit used therefor
WO1999010743A1 (en) * 1997-08-29 1999-03-04 Regents Of The University Of California Sol-gel matrices for direct colorimetric detection of analytes
EP0926497A2 (en) * 1997-12-26 1999-06-30 Hogy Medical Co., Ltd. Colorimetric sensor employing polydiacetylene membrane
WO1999060399A1 (en) * 1998-05-21 1999-11-25 Cornell Research Foundation, Inc. Liposome-enhanced test device and method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BAEUMNER A J ET AL: "Development of a new immunosensor for pesticide detection: a disposable system with liposome-enhancement and amperometric detection", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 13, 1998, pages 519 - 529, XP002217793, ISSN: 0956-5663 *
WARSINKE A ET AL: "Electrochemical immunoassays", FRESENIUS J ANAL CHEM, SPRINGER-VERLAG, HEIDELBERG, DE, vol. 366, no. 6-7, March 2000 (2000-03-01), pages 622 - 634, XP002267091 *

Also Published As

Publication number Publication date
EP1448991A2 (en) 2004-08-25
WO2002082078A2 (en) 2002-10-17

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