WO2002077293A3 - Detection of fungal pathogens using the polymerase chain reaction - Google Patents

Detection of fungal pathogens using the polymerase chain reaction Download PDF

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Publication number
WO2002077293A3
WO2002077293A3 PCT/EP2002/002581 EP0202581W WO02077293A3 WO 2002077293 A3 WO2002077293 A3 WO 2002077293A3 EP 0202581 W EP0202581 W EP 0202581W WO 02077293 A3 WO02077293 A3 WO 02077293A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
polymerase chain
fungal pathogens
chain reaction
tissue
Prior art date
Application number
PCT/EP2002/002581
Other languages
French (fr)
Other versions
WO2002077293A2 (en
Inventor
James Joseph Beck
Charles Jason Barnett
Christy Violet Perry
Original Assignee
Syngenta Participations Ag
James Joseph Beck
Charles Jason Barnett
Christy Violet Perry
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, James Joseph Beck, Charles Jason Barnett, Christy Violet Perry filed Critical Syngenta Participations Ag
Priority to EP02737879A priority Critical patent/EP1379699A2/en
Priority to IL15730502A priority patent/IL157305A0/en
Priority to JP2002575333A priority patent/JP2004520843A/en
Priority to AU2002312759A priority patent/AU2002312759B9/en
Publication of WO2002077293A2 publication Critical patent/WO2002077293A2/en
Publication of WO2002077293A3 publication Critical patent/WO2002077293A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to the use of primers in polymerase chain assays for the detection of fungal pathogens Colletotrichum acutatum, Alternaria spp., and Cladosporium carpophilum. Specific primers are indentified as being useful for the identification of fungal isolates using PCR based techniques. Also described are novel extraction buffer solutions for use in isolating DNA from an organism, methods of extracting DNA from tissue, and methods of performing PCR analysis on DNA extracted from tissue.
PCT/EP2002/002581 2001-03-09 2002-03-08 Detection of fungal pathogens using the polymerase chain reaction WO2002077293A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02737879A EP1379699A2 (en) 2001-03-09 2002-03-08 Detection of fungal pathogens using the polymerase chain reaction
IL15730502A IL157305A0 (en) 2001-03-09 2002-03-08 Detection of fungal pathogens using the polymerase chain reaction
JP2002575333A JP2004520843A (en) 2001-03-09 2002-03-08 Detection of fungal pathogens using polymerase chain reaction
AU2002312759A AU2002312759B9 (en) 2001-03-09 2002-03-08 Detection of fungal pathogens using the polymerase chain reaction

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US27454001P 2001-03-09 2001-03-09
US60/274,540 2001-03-09
US09/939,379 US6645720B2 (en) 2001-03-09 2001-08-24 Detection of fungal pathogens using the polymerase chain reaction
US09/939,379 2001-08-24

Publications (2)

Publication Number Publication Date
WO2002077293A2 WO2002077293A2 (en) 2002-10-03
WO2002077293A3 true WO2002077293A3 (en) 2003-11-20

Family

ID=26956887

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/002581 WO2002077293A2 (en) 2001-03-09 2002-03-08 Detection of fungal pathogens using the polymerase chain reaction

Country Status (6)

Country Link
US (3) US6645720B2 (en)
EP (1) EP1379699A2 (en)
JP (1) JP2004520843A (en)
AU (1) AU2002312759B9 (en)
IL (1) IL157305A0 (en)
WO (1) WO2002077293A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003085378A2 (en) * 2002-04-03 2003-10-16 Syngenta Participations Ag Detection of wheat and barley fungal pathogens which are resistant to certain fungicides using the polymerase chain reaction
US7291465B2 (en) * 2004-02-20 2007-11-06 Karaolis David K R Method for direct detection of fungal pathogens
KR100744699B1 (en) 2005-11-03 2007-08-01 충남대학교산학협력단 Specific primer for identifying and detecting Alternaria panax in Panax ginseng
EP2019687B1 (en) 2006-03-29 2014-03-19 Merial Limited Vaccine against streptococci
ES2326022B1 (en) * 2008-03-25 2010-06-07 Neuron Biopharma, S.A. IMPROVED PROCEDURE FOR BIODIESEL PRODUCTION.
CN102559923B (en) * 2012-03-20 2014-01-08 南京农业大学 Method of quickly identifying category of pathogenic bacteria of fungal diseases in chrysanthemum
WO2017205957A1 (en) * 2016-06-01 2017-12-07 9087-4405 Quebec Inc. Remote access system and method for plant pathogen management
RU2662930C1 (en) * 2017-06-01 2018-07-31 Федеральное государственное бюджетное учреждение науки Институт клеточного и внутриклеточного симбиоза Уральского отделения Российской академии наук System of monitoring of pathogenic potential of enterobacteria by polymerase chain reaction
FR3083248A1 (en) * 2018-06-28 2020-01-03 Anova-Plus KIT FOR DETECTION OF ALTERNARIOSIS AND DISCRIMINATION BETWEEN ALTERNARIA STRAINS OF THE ALTERNARIA SECTION AND THOSE OF THE PORRI SECTION
CN109852722A (en) * 2019-03-22 2019-06-07 浙江理工大学 Detect the method and the primer of dendrobium candidum black spot disease fungus dynamic change
JP7399451B2 (en) * 2019-10-02 2023-12-18 国立大学法人九州大学 Identification method and kit for rosaceans

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827695A (en) * 1997-08-04 1998-10-27 Novartis Finance Corporation Detection of wheat fungal pathogens using the polymerase chain reaction
WO1999042609A1 (en) * 1998-02-20 1999-08-26 Novartis Pharma Ag Detection of wheat and barley fungal pathogens using the polymerase chain reaction
EP0955381A2 (en) * 1994-04-25 1999-11-10 Novartis AG Detection of fungal pathogens using the polymerase chain reaction
WO2000052202A2 (en) * 1999-03-01 2000-09-08 Syngenta Participations Ag Detection of monilinia spp. using the polymerase chain reaction

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5800997A (en) 1996-11-01 1998-09-01 Novartis Finance Corporation Detection of maize fungal pathogens using the polymerase chain reaction
US5780271A (en) * 1996-11-13 1998-07-14 North Carolina State University PCR assays for phytophthora species

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0955381A2 (en) * 1994-04-25 1999-11-10 Novartis AG Detection of fungal pathogens using the polymerase chain reaction
US5827695A (en) * 1997-08-04 1998-10-27 Novartis Finance Corporation Detection of wheat fungal pathogens using the polymerase chain reaction
WO1999042609A1 (en) * 1998-02-20 1999-08-26 Novartis Pharma Ag Detection of wheat and barley fungal pathogens using the polymerase chain reaction
WO2000052202A2 (en) * 1999-03-01 2000-09-08 Syngenta Participations Ag Detection of monilinia spp. using the polymerase chain reaction

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 6 May 1994 (1994-05-06), "C. acutatum DNA for ITS1", XP002226078, retrieved from EBI Database accession no. Z32907 *
DATABASE EMBL [online] 6 May 1994 (1994-05-06), "C. acutatum DNA for ITS2", XP002226079, retrieved from EBI Database accession no. Z32914 *
SREENIVASAPRASAD, S. ET AL.: "Nucleotide sequence of the rDNA spacer 1 enables identification of isolates of Colletotrichum as C. acutatum", MYCOLOGICAL RESEARCH, vol. 98, no. 2, 1994, pages 186 - 188, XP009003482 *
SREENIVASAPRASAD, S. ET AL.: "Phylogeny and systematics of 18 Colletotrichum species based on ribosomal DNA spacer sequences", GENOME, vol. 39, no. 3, 1996, pages 499 - 512, XP001121466 *

Also Published As

Publication number Publication date
WO2002077293A2 (en) 2002-10-03
US6645720B2 (en) 2003-11-11
JP2004520843A (en) 2004-07-15
AU2002312759B2 (en) 2006-05-11
US20060166246A1 (en) 2006-07-27
AU2002312759B9 (en) 2006-06-08
US20030099946A1 (en) 2003-05-29
US7060816B2 (en) 2006-06-13
IL157305A0 (en) 2004-02-19
EP1379699A2 (en) 2004-01-14
US20040029255A1 (en) 2004-02-12

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