WO2002077185A2 - Methods for modifying flowering phenotypes - Google Patents

Methods for modifying flowering phenotypes Download PDF

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WO2002077185A2
WO2002077185A2 PCT/US2002/009141 US0209141W WO02077185A2 WO 2002077185 A2 WO2002077185 A2 WO 2002077185A2 US 0209141 W US0209141 W US 0209141W WO 02077185 A2 WO02077185 A2 WO 02077185A2
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sequence
plant
nucleotide sequence
polypeptide
protein
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PCT/US2002/009141
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French (fr)
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WO2002077185A3 (en
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T. Lynne Reuber
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Mendel Biotechnology, Inc.
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Priority to AU2002245718A priority Critical patent/AU2002245718A1/en
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Publication of WO2002077185A3 publication Critical patent/WO2002077185A3/en

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214Plastid transformation

Definitions

  • This invention relates to the field of plant biology. More particularly, the present invention pertains to compositions and methods for phenotypically modifying a plant.
  • Transcription factors can modulate gene expression, either increasing or decreasing (inducing or repressing) the rate of transcription. This modulation results in differential levels of gene expression at various developmental stages, in different tissues and cell types, and in response to different exogenous (e.g., environmental) and endogenous stimuli throughout the life cycle of the organism. Because transcription factors are key controlling elements of biological pathways, altering the expression levels of one or more transcription factors can change entire biological pathways in an organism. For example, manipulation of the levels of selected transcription factors may result in increased expression of economically useful proteins or metabolic chemicals in plants or in improvement in other agriculturally relevant characteristics. Conversely, blocked or reduced expression of a transcription factor may reduce biosynthesis of unwanted compounds or remove an undesirable trait.
  • manipulating transcription factor levels in a plant offers tremendous potential in agricultural biotechnology for modifying a plant's traits.
  • plants In order to maximize reproductive success, plants have evolved complex mechanisms to ensure that flowering occurs under favorable conditions.
  • Analysis of late flowering mutants and ecotypes in Arabidopsis has revealed that such mechanisms are based upon several genetic pathways which may contain 80 or more loci. Together these loci co-ordinate flowering time with environmental variables (e.g. day-length, temperature, light quality, and nutrient availability) and with the developmental stage of the plant .
  • transcription factors that regulate flowering phenotypes and in particular that regulate timing of the onset of reproductive development, the duration of the phase in which floral meristems are initiated, or the duration of time for which floral organs persist prior to their abscission, or the number of flowers generated on a plant. These transcription factors therefore are useful to manipulate flowering phenotypes of a plant.
  • the invention relates to a recombinant polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence encoding a polypeptide comprising a sequence selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 and 26, or a complementary nucleotide sequence thereof; (b) a nucleotide sequence encoding a polypeptide comprising a conservatively substituted variant of a polypeptide of (a); (c) a nucleotide sequence comprising a sequence selected from those of SEQ ID NO: 1, 3, 5, 7, 9, 11 , 13, 15, 17, 19, 21, 23 and 25, or a complementary nucleotide sequence thereof; (d) a nucleotide sequence comprising silent substitutions in a nucleotide sequence of (c); (e) a nucleotide sequence which hybridizes under stringent conditions over substantially the entire length of a nucleotide sequence of one
  • polynucleotides and polypeptides of the invention are also specifically included in the polynucleotides and polypeptides of the invention.
  • the recombinant polynucleotide may further comprise a constitutive, inducible, or tissue-specific promoter operably linked to the nucleotide sequence.
  • the invention also relates to compositions comprising at least two of the above described polynucleotides.
  • the invention is an isolated or recombinant polypeptide comprising a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotide described above.
  • polynucleotides and polypeptides are useful for modifying the flowering phenotypes of a plant, and in particular for modifying timing of the onset of reproductive development, the duration of the phase in which floral meristems are initiated, the duration of time for which floral organs persist prior to their abscission, or the number of flowers generated on a plant.
  • the invention is a transgenic plant comprising one or more of the above described recombinant polynucleotides.
  • the invention is a plant with altered expression levels of a polynucleotide described above or a plant with altered expression or activity levels of an above described polypeptide.
  • the invention relates to a cloning or expression vector comprising the isolated or recombinant polynucleotide described above or cells comprising the cloning or expression vector.
  • the invention relates to a composition produced by incubating a polynucleotide of the invention with a nuclease, a restriction enzyme, a polymerase, a polymerase and a primer, a cloning vector, or with a cell.
  • the invention relates to a method for producing a plant having a modified flowering phenotype, such as flowering time or flowering period. The method comprises altering the expression of an isolated or recombinant polynucleotide of the invention or altering the expression or activity of a polypeptide of the invention in a plant to produce a modified plant, and selecting the modified plant for a modified flowering time or flowering period phenotype.
  • the invention in another aspect, relates to a method of identifying a factor that is modulated by or interacts with a polypeptide encoded by a polynucleotide of the invention.
  • the method comprises expressing a polypeptide encoded by the polynucleotide in a plant and identifying at least one factor that is modulated by or interacts with the polypeptide.
  • the method for identifying modulating or interacting factors is by detecting binding by the polypeptide to a promoter sequence, or by detecting interactions between an additional protein and the polypeptide in a yeast two hybrid system, or by detecting expression of a factor by hybridization to a microarray, subtractive hybridization or differential display.
  • the invention is a method of identifying a molecule that modulates activity or expression of a polynucleotide or polypeptide of interest.
  • the method comprises placing the molecule in contact with a plant comprising the polynucleotide or polypeptide encoded by the polynucleotide of the invention and monitoring one or more of the expression level of the polynucleotide in the plant, the expression level of the polypeptide in the plant, and modulation of an activity of the polypeptide in the plant.
  • the invention relates to an integrated system, computer or computer readable medium comprising one or more character strings corresponding to a polynucleotide of the invention, or to a polypeptide encoded by the polynucleotide.
  • the integrated system, computer or computer readable medium may comprise a link between one or more sequence strings to a modified plant flowering phenotype.
  • the invention is a method for identifying a sequence similar or homologous to one or more polynucleotides of the invention, or one or more polypeptides encoded by the polynucleotides.
  • the method comprises providing a sequence database and querying the sequence database with one or more target sequences corresponding to the one or more polynucleotides or to the one or more polypeptides to identify one or more sequence members of the database that display sequence similarity or homology to one or more of the target sequences.
  • the method may further comprise linking the one or more polynucleotides of the invention, or encoded polypeptides, to a modified plant flowering phenotype.
  • Sequence Listing provides exemplary polynucleotide and polypeptide sequences of the invention. These sequences may be employed to modify the flowering time or the flowering period of a plant.
  • the present invention relates to polynucleotides and polypeptides, e.g. for modifying phenotypes of plants.
  • the polynucleotides or polypeptides are useful for modifying traits associated with a plant's flowering time or flowering period when the expression levels of the polynucleotides or expression levels or activity levels of the polypeptides are altered compared with those found in a wild type plant.
  • the flowering time of plants can be either decreased, increased or made inducible under specific conditions using the polynucleotides or polypeptides of this invention.
  • polynucleotides and polypeptides are also useful for modifying the duration of the phase in which floral meristems are initiated, the duration of time for which floral organs persist prior to their abscission, or the number of flowers generated on a plant. Additionally, the polynucleotides and polypeptides are useful for modifying traits associated with modified vernalization requirements or flowering time characteristics, such as changes in flowering time in response to day-length, in response to temperature, in response to light quality, nutrient availability, and development stage of the plant, the length of flowering time which delays senescence and the like.
  • the polynucleotides of the invention encode plant transcription factors.
  • the plant transcription factors are derived, e.g., from Arabidopsis thaliana and can belong, e.g., to one or more of the following transcription factor families: the AP2
  • the polynucleotides and polypeptides of the invention have a variety of additional uses. These uses include their use in the recombinant production (i.e, expression) of proteins; as regulators of plant gene expression, as diagnostic probes for the presence of complementary or partially complementary nucleic acids (including for detection of natural coding nucleic acids), as substrates for further reactions, e.g., mutation reactions, PCR reactions, or the like, as substrates for cloning e.g., including digestion or ligation reactions, and for identifying exogenous or endogenous modulators of the transcription factors.
  • additional uses include their use in the recombinant production (i.e, expression) of proteins; as regulators of plant gene expression, as diagnostic probes for the presence of complementary or partially complementary nucleic acids (including for detection of natural coding nucleic acids), as substrates for further reactions, e.g., mutation reactions, PCR reactions, or the like, as substrates for cloning e.g., including digestion or ligation
  • a "polynucleotide” is a nucleic acid sequence comprising a plurality of polymerized nucleotide residues, e.g., at least about 15 consecutive polymerized nucleotide residues, optionally at least about 30 consecutive nucleotides, or at least about 50 consecutive nucleotides.
  • a polynucleotide comprises a nucleotide sequence encoding a polypeptide (or protein) or a domain or fragment thereof.
  • the polynucleotide may comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 5' or 3' untranslated regions, a reporter gene, a selectable marker, a purification tag, or the like.
  • the polynucleotide can be single stranded or double stranded DNA or RNA.
  • the polynucleotide optionally comprises modified bases or a modified backbone.
  • the polynucleotide can be, e.g., genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like.
  • the polynucleotide can comprise a sequence in either sense or antisense orientations.
  • a "recombinant polynucleotide” is a polynucleotide that is not in its native state, e.g., the polynucleotide comprises a nucleotide sequence not found in nature, or the polynucleotide is in a context other than that in which it is naturally found, e.g., separated from nucleotide sequences with which it typically is in proximity in nature, or adjacent (or contiguous with) nucleotide sequences with which it typically is not in proximity.
  • the sequence at issue can be cloned into a vector, or otherwise recombined with one or more additional nucleic acid.
  • isolated polynucleotide is a polynucleotide whether naturally occurring or recombinant, that is present outside the cell in which it is typically found in nature, whether purified or not.
  • an isolated polynucleotide is subject to one or more enrichment or purification procedures, e.g., cell lysis, extraction, centrifugation, precipitation, or the like.
  • a “recombinant polypeptide” is a polypeptide produced by translation of a recombinant polynucleotide.
  • the isolated polypeptide is separated from other cellular components with which it is typically associated, e.g., by any of the various protein purification methods herein.
  • the term "transgenic plant” refers to a plant that contains genetic material, not found in a wild type plant of the same species, variety or cultivar.
  • the genetic material may include a transgene, an insertional mutagenesis event (such as by transposon or T-DNA insertional mutagenesis), an activation tagging sequence, a mutated sequence, a homologous recombination event or a sequence modified by chimeraplasty.
  • the foreign genetic material has been introduced into the plant by human manipulation.
  • a transgenic plant may contain an expression vector or cassette.
  • the expression cassette typically comprises a polypeptide-encoding sequence operably linked (i.e., under regulatory control of) to appropriate inducible or constitutive regulatory sequences that allow for the expression of polypeptide.
  • the expression cassette can be introduced into a plant by transformation or by breeding after transformation of a parent plant.
  • a plant refers to a whole plant as well as to a plant part, such as seed, fruit, leaf, or root, plant tissue, plant cells or any other plant material, e.g., a plant explant, as well as to progeny thereof, and to in vitro systems that mimic biochemical or cellular components or processes in a cell.
  • ectopically expression or altered expression indicates that the pattern of expression in, e.g., a transgenic plant or plant tissue, is different from the expression pattern in a wild type plant or a reference plant of the same species.
  • the polynucleotide or polypeptide is expressed in a cell or tissue type other than a cell or tissue type in which the sequence is expressed in the wild type plant, or by expression at a time other than at the time the sequence is expressed in the wild type plant, or by a response to different inducible agents, such as hormones or environmental signals, or at different expression levels (either higher or lower) compared with those found in a wild type plant.
  • the term also refers to altered expression patterns that are produced by lowering the levels of expression to below the detection level or completely abolishing expression.
  • the resulting expression pattern can be transient or stable, constitutive or inducible.
  • the term "ectopic expression or altered expression” may further relate to altered activity levels resulting from the interactions of the polypeptides with exogenous or endogenous modulators or from interactions with factors or as a result of the chemical modification of the polypeptides.
  • fragment refers to a subsequence of the polypeptide.
  • the fragment or domain is a subsequence of the polypeptide which performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide.
  • a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA binding domain that binds to a DNA promoter region, an activation domain or a domain for protein-protein interactions.
  • Fragments can vary in size from as few as 5, 6 or 8 amino acids to the full length of the intact polypeptide, but are preferably at least about 30 amino acids in length and more preferably at least about 60 amino acids in length.
  • a fragment refers to any subsequence of a polynucleotide, typically, of at least consecutive about 15 nucleotides, encoding 5, 6, 8, or 10 amino acids for example, preferably at least about 30 nucleotides, more preferably at least about 50, of any of the sequences provided herein.
  • a fragment can consist of or comprise nucleotides encoding amino acids outside of a conserved domain known to exist in a particular transcription factor belonging to a transcription factor family, for example.
  • trait refers to a physiological, morphological, biochemical or physical characteristic of a plant or particular plant material or cell. In some instances, this characteristic is visible to the human eye, such as seed or plant size, or can be measured by available biochemical techniques, such as the protein, starch or oil content of seed or leaves or by the observation of the expression level of genes, e.g., by employing Northern analysis, RT-PCR, microarray gene expression assays or reporter gene expression systems, or by agricultural observations such as stress tolerance, yield or pathogen tolerance.
  • Trait modification refers to a detectable difference in a characteristic in a plant ectopically expressing a polynucleotide or polypeptide of the present invention relative to a plant not doing so, such as a wild type plant.
  • the trait modification can be evaluated quantitatively.
  • the trait modification can entail at least about a 2% increase or decrease in an observed trait (difference), at least about a 5% difference, at least about a 10% difference, at least about a 20% difference, at least about a 30%, at least about a 50%, at least about a 70%, or at least about a 100%, or an even greater difference. It is known that there can be a natural variation in the modified trait. Therefore, the trait modification observed entails a change of the normal distribution of the trait in the plants compared with the distribution observed in wild type plant.
  • Trait modifications of particular interest include those to seed (such as embryo or endosperm), fruit, root, flower, leaf, stem, shoot, seedling or the like, including: enhanced tolerance to environmental conditions including freezing, chilling, heat, drought, water saturation, radiation and ozone; improved tolerance to microbial, fungal or viral diseases; improved tolerance to pest infestations, including nematodes, mollicutes, parasitic higher plants or the like; decreased herbicide sensitivity; improved tolerance of heavy metals or enhanced ability to take up heavy metals; improved growth under poor photoconditions (e.g., low light and/or short day length); or changes in expression levels of genes of interest.
  • seed such as embryo or endosperm
  • fruit root, flower, leaf, stem, shoot, seedling or the like
  • enhanced tolerance to environmental conditions including freezing, chilling, heat, drought, water saturation, radiation and ozone
  • improved tolerance to microbial, fungal or viral diseases improved tolerance to pest infestations, including nematodes, mollicutes, parasitic higher plants or the like
  • phenotypes that can be modified relate to the production of plant metabolites, such as variations in the production of taxol, tocopherol, tocotrienol, sterols, phytosterols, vitamins, wax monomers, anti-oxidants, amino acids, lignins, cellulose, tannins, prenyllipids (such as chlorophylls and carotenoids), glucosinolates, and terpenoids, enhanced or compositionally altered protein or oil production (especially in seeds), or modified sugar (insoluble or soluble) and/or starch composition.
  • Physical plant characteristics that can be modified include cell development (such as the number of trichomes), fruit and seed size and number, yields of plant parts such as stems, leaves and roots, the stability of the seeds during storage, characteristics of the seed pod (e.g., susceptibility to shattering), root hair length and quantity, internode distances, or the quality of seed coat.
  • Plant growth characteristics that can be modified include growth rate, germination rate of seeds, vigor of plants and seedlings, leaf and flower senescence, male sterility, apomixis, flowering time, flowering period, flower abscission, rate of nitrogen uptake, biomass or transpiration characteristics, as well as plant architecture characteristics such as apical dominance, branching patterns, number of organs, organ identity, organ shape or size.
  • the present invention provides, among other things, transcription factors (TFs), and transcription factor homologue polypeptides, and isolated or recombinant polynucleotides encoding the polypeptides. These polypeptides and polynucleotides may be employed to modify a plant's flowering phenotype.
  • Exemplary polynucleotides encoding the polypeptides of the invention were identified in the Arabidopsis thaliana GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. Polynucleotide sequences meeting such criteria were confirmed as transcription factors.
  • Additional polynucleotides of the invention were identified by screening Arabidopsis thaliana and/or other plant cDNA libraries with probes corresponding to known transcription factors under low stringency hybridization conditions. Additional sequences, including full length coding sequences were subsequently recovered by the rapid amplification of cDNA ends (RACE) procedure, using a commercially available kit according to the manufacturer's instructions. Where necessary, multiple rounds of RACE are performed to isolate 5' and 3' ends. The full length cDNA was then recovered by a routine end-to-end polymerase chain reaction (PCR) using primers specific to the isolated 5' and 3' ends. Exemplary sequences are provided in the Sequence Listing.
  • polynucleotides of the invention were ectopically expressed in overexpressor or knockout plants and changes in the flowering phenotype of the plants was observed. Therefore, the polynucleotides and polypeptides can be employed to improve the flowering phenotype of plants.
  • the polynucleotides of the invention include sequences that encode transcription factors and transcription factor homologue polypeptides and sequences complementary thereto, as well as unique fragments of coding sequence, or sequence complementary thereto.
  • Such polynucleotides can be, e.g., DNA or RNA, mRNA, cRNA, synthetic RNA, genomic DNA, cDNA synthetic DNA, oligonucleotides, etc.
  • the polynucleotides are either double-stranded or single-stranded, and include either, or both sense (i.e., coding) sequences and antisense (i.e., non-coding, complementary) sequences.
  • the polynucleotides include the coding sequence of a transcription factor, or transcription factor homologue polypeptide, in isolation, in combination with additional coding sequences (e.g., a purification tag, a localization signal, as a fusion- protein, as a pre-protein, or the like), in combination with non-coding sequences (e.g., introns or inteins, regulatory elements such as promoters, enhancers, terminators, and the like), and/or in a vector or host environment in which the polynucleotide encoding a transcription factor or transcription factor homologue polypeptide is an endogenous or exogenous gene.
  • additional coding sequences e.g., a purification tag, a localization signal, as a fusion- protein, as a pre-protein, or the like
  • non-coding sequences e.g., introns or inteins, regulatory elements such as promoters, enhancers, terminators, and the like
  • polynucleotides o the invention can be produced by a variety of in vitro amplification methods adapted to the present invention by appropriate selection of specific or degenerate primers.
  • protocols sufficient to direct persons of skill through in vitro amplification methods including the polymerase chain reaction (PCR) the ligase chain reaction (LCR), Qbeta-replicase amplification and other RNA polymerase mediated techniques (e.g., NASBA), e.g., for the production of the homologous nucleic acids of the invention are found in Berger, Sambrook, and Ausubel, as well as Mullis et al., (1987) PCR Protocols A Guide to Methods and Applications (Innis et al.
  • RNA can be converted into a double stranded DNA suitable for restriction digestion, PCR expansion and sequencing using reverse transcriptase and a polymerase. See, e.g., Ausubel, Sambrook and Berger, supra.
  • polynucleotides and oligonucleotides of the invention can be assembled from fragments produced by solid-phase synthesis methods. Typically, fragments of up to approximately 100 bases are individually synthesized and then enzymatically or chemically ligated to produce a desired sequence, e.g., a polynucletotide encoding all or part of a transcription factor.
  • a desired sequence e.g., a polynucletotide encoding all or part of a transcription factor.
  • chemical synthesis using the phosphoramidite method is described, e.g., by Beaucage et al. (1981) Tetrahedron Letters 22: 1859-69; and Matthes et al. (1984) EMBO J. 3:801-5.
  • oligonucleotides are synthesized, purified, annealed to their complementary strand, ligated and then optionally cloned into suitable vectors. And if so desired, the polynucleotides and polypeptides of the invention can be custom ordered from any of a number of commercial suppliers.
  • Sequences homologous, i.e., that share significant sequence identity or similarity, to those provided in the Sequence Listing, derived from Arabidopsis thaliana or from other plants of choice are also an aspect of the invention.
  • Homologous sequences can be derived from any plant including monocots and dicots and in particular agriculturally important plant species including, but not limited to, crops such as soybean, wheat, corn, potato, cotton, rice, oilseed rape (including canola), sunflower, alfalfa, sugarcane and turf; or fruits and vegetables, such as banana, blackberry, blueberry, strawberry, and raspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant, grapes, honeydew, lettuce, mango, melon, onion, papaya, peas, peppers, pineapple, spinach, squash, sweet corn, tobacco, tomato, watermelon, rosaceous fruits (such as apple, peach, pear, cherry and plum) and vegetable brassicas (such as broccoli, cabbage, cauliflower, brussel sprouts and kohlrabi).
  • crops such as soybean, wheat, corn, potato, cotton, rice, oilseed rape (including canola), sunflower, alfalfa, sugarcane and turf
  • fruits and vegetables such as banana, black
  • Other crops, fruits and vegetables whose phenotype can be changed include barley, rye, millet, sorghum, currant, avocado, citrus fruits such as oranges, lemons, grapefruit and tangerines, artichoke, cherries, nuts such as the walnut and peanut, endive, leek, roots, such as arrowroot, beet, cassava, turnip, radish, yam, and sweet potato, and beans.
  • the homologous sequences may also be derived from woody species, such pine, poplar and eucalyptus. Transcription factors that are homologous to the listed sequences will typically share at least about 35% amino acid sequence identity.
  • More closely related transcription factors can share at least about 50%, about 60%, about 65%, about 70%, about 75% or about 80% or about 90% or about 95% or about 98% or more sequence identity with the listed sequences.
  • Factors that are most closely related to the listed sequences share, e.g., at least about 85%, about 90% or about 95% or more sequence identity to the listed sequences.
  • the sequences will typically share at least about 40% nucleotide sequence identity, preferably at least about 50%, about 60%, about 70% or about 80% sequence identity, and more preferably about 85%, about 90%, about 95% or about 97% or more sequence identity to one or more of the listed sequences.
  • the degeneracy of the genetic code enables major variations in the nucleotide sequence of a polynucleotide while maintaining the amino acid sequence of the encoded protein.
  • conserveed domains within a transcription factor family may exhibit a higher degree of sequence homology, such as at least 65% sequence identity including conservative substitutions, and preferably at least 80% sequence identity.
  • Exemplary conserved domains of the present invention include: for G2010 (SEQ ID NO: 7 and 8) amino acid residues 54 through 127, for G1037 (SEQ ID NO: 9 and 10) amino acid residues 11 through 134 or 200 through 248, for G1820 (SEQ ID NO: 5 and 6) amino acid residues 41-159, for G 1760 (SEQ ID NO: 3 and 4) amino acid residues 2 through 57, and for G590 (SEQ ID NO: 1 and 2) amino acid residues 193 through 253.
  • Transcription factors of the invention can also contain 5, 6, 8, 10 or 12 consecutive amino acids from the sequences of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 where the consecutive amino acids are taken from a region outside of the conserved domain.
  • Polynucleotides having at least 90%, or at least 85%, or at least 75%, or at least 60%, or at least 50%, or at least 40% sequence identity to those encoding the above transcription factors are also included in this invention. Orthologs and
  • orthologs and paralogs are evolutionarily related genes that have similar sequences and similar functions. Paralogs are related genes within a single species and are most likely a result of gene duplication, whereas orthologs are related genes in different species derived from a common ancestral molecule prior to speciation. Within a single plant species, gene duplication may cause two copies of a particular gene, giving rise to two or more genes with similar sequence and similar function known as paralogs.
  • a paralog is therefore a similar gene with a similar function within the same species.
  • Paralogs typically cluster together or in the same clade (a group of similar genes), as is shown when a gene family phylogeny is analyzed using programs such as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22:4673-4680; Higgins et al. (1996) Methods Enzymol. 266 383-402). Groups of similar genes can also be identified using by pair-wise BLAST analysis (Feng and Doolittle (1987) J. Mol. Evol. 25:351-360).
  • a clade of very similar MADS domain transcription factors from Arabidopsis all share a common function in flowering time (Ratcliffe et al. (2001) Plant Physiol. 126: 122-132), and a group of very similar AP2 domain transcription factors from Arabidopsis are involved in tolerance of plants to freezing (Gilmour et al. (1998) Plant J. 16:433-442).
  • Analysis of groups of similar genes with similar function that fall within one clade can yield subsequences that are particular to the clade. These subsequences, known as consensus sequences, can not only be used to define the sequences within each clade, but define the functions of these genes, since genes within each clade typically share the same function. (See also, for example, Mount, D.W. (2001) Bioinformatics: Sequence and Genome Analysis Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York page 543.)
  • orthologs genes with similar sequence and similar function. These genes, termed orthologs, often have an identical function within their host plants and are often interchangeable between species without losing function. Because plants have common ancestors, many genes in any plant species will have a corresponding orthologous gene in another plant species.
  • orthologous sequences can placed into the phylogenetic tree and their relationship to genes from the species of interest can be determined. Once the ortholog pair has been identified, the function of the test ortholog can be determined by determining the function of the reference ortholog. Orthologs can also be identified by pair-wise BLAST analysis by aligning a set of reference sequences against a set of test sequences. Test sequences with the closest match to a particular reference sequence, as determined by the P- value of the BLAST analysis, can be taken and individually aligned against the reference set of sequences. The individual test sequence will either best match the particular reference sequence, in which case it is likely to be an ortholog, or not, in which case it may not be an ortholog.
  • a further way of identifying an ortholog is by identifying a consensus sequence within the candidate ortholog. Using pair-wise BLAST analysis, or programs such as CLUSTAL alignment program, sets of similar genes, or clades, can be identified. The particular subsequences which define commonalities within a particular clade can be derived from an alignment of those sequences. Orthologs would have the consensus sequence, or a sequence similar to the consensus sequence. Orthologs might also have a consensus sequence outside a conserved domain, which could be particular to that family of orthologous sequences.
  • Corresponding orthologs may bridge the monocot/dicot division of the plant kingdom and orthologous pairs of genes can be identified in rice and Arabidopsis, corn and Arabidopsis and Antirhinnum and com.
  • Peng et al showed that a mutant of the Arabidopsis gene termed Gibberellin fnsensitive (GAI; mutant termed gai) encoded a transcription factor and which conferred a reduction in gibberellin responsiveness in the native plant (Peng et al. 1997 Genes and Development 11 :3194-3205).
  • GAI Gibberellin fnsensitive
  • the Arabidopsis GAI protein has 62 % amino acid residue identity with the wheat Rht- Dla protein and 62 % amino acid residue identity with the maize d8.
  • Peng et al. showed that transgenic rice plants containing a mutant GAI allele give reduced response to gibberellin and are dwarfed, mimicking the dwarfed wheat variety from which the mutant Rht-Dla gene was isolated.
  • Peng et al. taught that Arabidopsis GAI protein is an ortholog of the wheat Rht-Dla and maize d8 proteins. (Peng et al. 1999 Nature 400:256-261.)
  • Table 1 (appended to this application) lists a summary of orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search.
  • the first column shows the polynucleotide sequence identifier (SEQ ID NO)
  • the second column shows the transcription factor cDNA identifier (Gene ID)
  • the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide sequence identified in a BLAST search (Test Sequence ID)
  • the fourth column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability)
  • the fifth column identifies the plant species of the Test Sequence (Test Sequence Species)
  • the sixth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation).
  • Table 2 (appended to this application) lists orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search.
  • the first column shows the polynucleotide sequence identifier (SEQ ID NO)
  • the second column shows the transcription factor cDNA identifier (Gene ID)
  • the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide (Test Sequence ID)
  • the fourth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation)
  • the fifth column shows the reading frame of the Test sequence encoding the orthologous or homologous sequence (Reading Frame)
  • the sixth column shows the calculated score value of the aligned sequences (High Score)
  • the seventh column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability)
  • the eighth column shows the number of regions in the Test Sequence that align with a sequence
  • Sequence Listing can be identified in a variety of ways known to one skilled in the art, e.g., by hybridization to each other under stringent or under highly stringent conditions.
  • Single stranded polynucleotides hybridize when they associate based on a variety of well characterized physico-chemical forces, such as hydrogen bonding, solvent exclusion, base stacking and the like.
  • the stringency of a hybridization reflects the degree of sequence identity of the nucleic acids involved, such that the higher the stringency, the more similar are the two polynucleotide strands. Stringency is influenced by a variety of factors, including temperature, salt concentration and composition, organic and non-organic additives, solvents, etc.
  • An example of stringent hybridization conditions for hybridization of complementary nucleic acids which have more than 100 complementary residues on a filter in a Southern or northern blot is about 5°C to 20°C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH.
  • Tm is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a perfectly matched probe.
  • Nucleic acid molecules that hybridize under stringent conditions will typically hybridize to a probe based on either the entire cDNA or selected portions, e.g., to a unique subsequence, of the cDNA under wash conditions of 0.2x SSC to 2.0 x SSC, 0.1% SDS at 50-65° C, for example 0.2 x SSC, 0.1% SDS at 65° C.
  • stringency is increased by raising the wash temperature and/or decreasing the concentration of SSC.
  • stringent conditions can be selected such that an oligonucleotide that is perfectly complementary to the coding oligonucleotide hybridizes to the coding oligonucleotide with at least about a 5-10x higher signal to noise ratio than the ratio for hybridization of the perfectly complementary oligonucleotide to a nucleic acid encoding a transcription factor known as of the filing date of the application.
  • Conditions can be selected such that a higher signal to noise ratio is observed in the particular assay which is used, e.g., about 15x, 25x, 35x, 50x or more.
  • the subject nucleic acid hybridizes to the unique coding oligonucleotide with at least a 2x higher signal to noise ratio as compared to hybridization of the coding oligonucleotide to a nucleic acid encoding known polypeptide.
  • higher signal to noise ratios can be selected, e.g., about 5x, lOx, 25x, 35x, 50x or more.
  • the particular signal will depend on the label used in the relevant assay, e.g., a fluorescent label, a colorimetric label, a radio active label, or the like.
  • transcription factor homologue polypeptides can be obtained by screening an expression library using antibodies specific for one or more transcription factors.
  • the encoded polypeptide(s) can be expressed and purified in a heterologous expression system (e.g., E. coli) and used to raise antibodies (monoclonal or polyclonal) specific for the polypeptide(s) in question.
  • Antibodies can also be raised against synthetic peptides derived from transcription factor, or transcription factor homologue, amino acid sequences. Methods of raising antibodies are well known in the art and are described in Harlow and Lane (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, New York. Such antibodies can then be used to screen an expression library produced from the plant from which it is desired to clone additional transcription factor homologues, using the methods described above.
  • the selected cDNAs can be confirmed by sequencing and enzymatic activity.
  • any of a variety of polynucleotide sequences are capable of encoding the transcription factors and transcription factor homologue polypeptides of the invention. Due to the degeneracy of the genetic code, many different polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing.
  • Table 3 illustrates, e.g., that the codons AGC, AGT, TCA, TCC, TCG, and TCT all encode the same amino acid: serine. Accordingly, at each position in the sequence where there is a codon encoding serine, any of the above trinucleotide sequences can be used without altering the encoded polypeptide.
  • conservative variations that alter one, or a few amino acids in the encoded polypeptide, can be made without altering the function of the polypeptide, these conservative variants are, likewise, a feature of the invention.
  • substitutions, deletions and insertions introduced into the sequences provided in the Sequence Listing are also envisioned by the invention.
  • Such sequence modifications can be engineered into a sequence by site-directed mutagenesis (Wu (ed.) Meth. Enzymol. (1993) vol. 217, Academic Press) or the other methods noted below.
  • Amino acid substitutions are typically of single residues; insertions usually will be on the order of about from 1 to 10 amino acid residues; and deletions will range about from 1 to 30 residues.
  • deletions or insertions are made in adjacent pairs, e.g., a deletion of two residues or insertion of two residues. Substitutions, deletions, insertions or any combination thereof can be combined to arrive at a sequence.
  • the mutations that are made in the polynucleotide encoding the transcription factor should not place the sequence out of reading frame and should not create complementary regions that could produce secondary mRNA structure.
  • the polypeptide encoded by the DNA performs the desired function.
  • Conservative substitutions are those in which at least one residue in the amino acid sequence has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the Table 4 when it is desired to maintain the activity of the protein.
  • Table 4 shows amino acids which can be substituted for an amino acid in a protein and which are typically regarded as conservative substitutions.
  • Substitutions that are less conservative than those in Table 4 can be selected by picking residues that differ more significantly in their effect on maintaining (a) the structure of the polypeptide backbone in the area of the substitution, for example, as a sheet or helical conformation, (b) the charge or hydrophobicity of the molecule at the target site, or (c) the bulk of the side chain.
  • substitutions which in general are expected to produce the greatest changes in protein properties will be those in which (a) a hydrophilic residue, e.g., seryl or threonyl, is substituted for (or by) a hydrophobic residue, e.g., leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain, e.g., lysyl, arginyl, or histidyl, is substituted for (or by) an electronegative residue, e.g., glutamyl or aspartyl; or (d) a residue having a bulky side chain, e.g., phenylalanine, is substituted for (or by) one not having a side chain, e.g., glycine.
  • a hydrophilic residue e.g
  • the present invention optionally includes methods of modifying the sequences of the Sequence Listing.
  • nucleic acid or protein modification methods are used to alter the given sequences to produce new sequences and/or to chemically or enzymatically modify given sequences to change the properties of the nucleic acids or proteins.
  • given nucleic acid sequences are modified, e.g., according to standard mutagenesis or artificial evolution methods to produce modified sequences.
  • Ausubel, supra provides additional details on mutagenesis methods.
  • Artificial forced evolution methods are described, e.g., by Stemmer (1994) Nature 370:389-391, and Stemmer (1994) Proc. Natl. Acad. Sci. USA 91 : 10747- 10751. Many other mutation and evolution methods are also available and expected to be within the skill of the practitioner.
  • chemical or enzymatic alteration of expressed nucleic acids and polypeptides can be performed by standard methods.
  • sequence can be modified by addition of lipids, sugars, peptides, organic or inorganic compounds, by the inclusion of modified nucleotides or amino acids, or the like.
  • protein modification techniques are illustrated in Ausubel, supra. Further details on chemical and enzymatic modifications can be found herein. These modification methods can be used to modify any given sequence, or to modify any sequence produced by the various mutation and artificial evolution modification methods noted herein.
  • the invention provides for modification of any given nucleic acid by mutation, evolution, chemical or enzymatic modification, or other available methods, as well as for the products produced by practicing such methods, e.g., using the sequences herein as a starting substrate for the various modification approaches.
  • optimized coding sequence containing codons preferred by a particular prokaryotic or eukaryotic host can be used e.g., to increase the rate of translation or to produce recombinant RNA transcripts having desirable properties, such as a longer half-life, as compared with transcripts produced using a on- optimized sequence.
  • Translation stop codons can also be modified to reflect host preference. For example, preferred stop codons for S. cerevisiae and mammals are TAA and TGA, respectively. The preferred stop codon for monocotyledonous plants is TGA, whereas insects and E. coli prefer to use TAA as the stop codon.
  • polynucleotide sequences of the present invention can also be engineered in order to alter a coding sequence for a variety of reasons, including but not limited to, alterations which modify the sequence to facilitate cloning, processing and/or expression of the gene product.
  • alterations are optionally introduced using techniques which are well known in the art, e.g., site-directed mutagenesis, to insert new restriction sites, to alter glycosylation patterns, to change codon preference, to introduce splice sites, etc.
  • a fragment or domain derived from any of the polypeptides of the invention can be combined with domains derived from other transcription factors or synthetic domains to modify the biological activity of a transcription factor.
  • a DNA binding domain derived from a transcription factor of the invention can be combined with the activation domain of another transcription factor or with a synthetic activation domain.
  • a transcription activation domain assists in initiating transcription from a DNA binding site. Examples include the transcription activation region of VP16 or GAL4 (Moore et al. (1998) Proc. Natl. Acad. Sci. USA 95: 376-381; and Aovama et al.
  • polynucleotide sequences of the invention are inco ⁇ orated into recombinant DNA (or RNA) molecules that direct expression of polypeptides of the invention in appropriate host cells, transgenic plants, in vitro translation systems, or the like. Due to the inherent degeneracy of the genetic code, nucleic acid sequences which encode substantially the same or a functionally equivalent amino acid sequence can be substituted for any listed sequence to provide for cloning and expressing the relevant homologue.
  • the present invention includes recombinant constructs comprising one or more of the nucleic acid sequences herein.
  • the constructs typically comprise a vector, such as a plasmid, a cosmid, a phage, a virus (e.g., a plant virus), a bacterial artificial chromosome (BAC), a yeast artificial chromosome (YAC), or the like, into which a nucleic acid sequence of the invention has been inserted, in a forward or reverse orientation.
  • the construct further comprises regulatory sequences, including, for example, a promoter, operably linked to the sequence. Large numbers of suitable vectors and promoters are known to those of skill in the art, and are commercially available.
  • non-Ti vectors can be used to transfer the DNA into monocotyledonous plants and cells by using free DNA delivery techniques. Such methods can involve, for example, the use of liposomes, electroporation, microprojectile bombardment, silicon carbide whiskers, and viruses.
  • transgenic plants such as wheat, rice (Christou (1991) Bio/Technology 9: 957-962) and com (Gordon-Kamm (1990) Plant Cell 2: 603-618) can be produced.
  • An immature embryo can also be a good target tissue for monocots for direct DNA delivery techniques by using the particle gun (Weeks et al. (1993) Plant Physiol 102: 1077-1084; Vasil (1993) Bio/Technology 10: 667-674; Wan and Lemeaux (1994) Plant Physiol 104: 37-48, and for Agrobacterium-mediated DNA transfer (Ishida et al. (1996) Nature Biotech 14: 745-750).
  • plant transformation vectors include one or more cloned plant coding sequence (genomic or cDNA) under the transcriptional control of 5' and 3' regulatory sequences and a dominant selectable marker.
  • plant transformation vectors typically also contain a promoter (e.g., a regulatory region controlling inducible or constitutive, environmentally-or developmentally-regulated, or cell- or tissue-specific expression), a transcription initiation start site, an RNA processing signal (such as intron splice sites), a transcription termination site, and/or a polyadenylation signal.
  • constitutive plant promoters which can be useful for expressing the TF sequence include: the cauliflower mosaic vims (CaMV) 35S promoter, which confers constitutive, high-level expression in most plant tissues (see, e.g., Odel et al. (1985) Nature 313:810); the nopaline synthase promoter (An et al.
  • a variety of plant gene promoters that regulate gene expression in response to environmental, hormonal, chemical, developmental signals, and in a tissue-specific or preferential manner can be used for expression of a TF sequence in plants.
  • Choice of a promoter is based largely on the phenotype of interest and is determined by such factors as tissue (e.g., seed, fruit, root, pollen, vascular tissue, flower, ca ⁇ el, etc.), inducibility (e.g., in response to wounding, heat, cold, drought, light, pathogens, etc.), timing, developmental stage, and the like.
  • tissue e.g., seed, fruit, root, pollen, vascular tissue, flower, ca ⁇ el, etc.
  • inducibility e.g., in response to wounding, heat, cold, drought, light, pathogens, etc.
  • timing, developmental stage, and the like e.g., developmental stage, and the like.
  • Numerous known promoters have been characterized and can favorable be employed to promote expression of a
  • tissue specific promoters include: seed-specific promoters (such as the napin, phaseolin or DC3 promoter described in US Pat. No. 5,773,697), fruit-specific promoters that are active during fruit ripening (such as the dru 1 promoter (US Pat. No. 5,783,393), or the 2A1 1 promoter (US Pat. No. 4,943,674) and the tomato polygalacturonase promoter (Bird et al. (1988) Plant Mol Biol 1 1 :651), root-specific promoters, such as those disclosed in US Patent Nos.
  • seed-specific promoters such as the napin, phaseolin or DC3 promoter described in US Pat. No. 5,773,697
  • fruit-specific promoters that are active during fruit ripening such as the dru 1 promoter (US Pat. No. 5,783,393), or the 2A1 1 promoter (US Pat. No. 4,943,674)
  • the tomato polygalacturonase promoter (B
  • pollen-active promoters such as PTA29, PTA26 and PTA13 (US Pat. No. 5,792,929), promoters active in vascular tissue (Ringli and Keller (1998) Plant Mol Biol 37:977-988), flower-specific (Kaiser et al, (1995) Plant Mol Biol 28:231-243). pollen (Baerson et al. (1994) Plant Mol Biol 26: 1947-1959), ca ⁇ els (Ohl et al. (1990) Plant Cell 2:837-848), pollen and ovules (Baerson et al.
  • auxin-inducible promoters such as that described in van der Kop et al. (1999) Plant Mol Biol 39:979-990 or Baumann et al. (1999) Plant Cell 11 :323-334
  • cytokinin-inducible promoter Guevara-Garcia (1998) Plant Mol Biol 38:743-753
  • promoters responsive to gibberellin Shi et al. (1998) Plant Mol Biol 38:1053-1060, Willmott et al. (1998) 38:817-825) and the like.
  • Additional promoters are those that elicit expression in response to heat (Ainley et al.
  • timing of the expression can be controlled by using promoters such as those acting at senescence (Gan and
  • Plant expression vectors can also include RNA processing signals that can be positioned within, upstream or downstream of the coding sequence.
  • the expression vectors can include additional regulatory sequences from the 3 '-untranslated region of plant genes, e.g., a 3' terminator region to increase mRNA stability of the mRNA, such as the PI-II terminator region of potato or the octopine or nopaline synthase 3' terminator regions.
  • initiation signals can aid in efficient translation of coding sequences. These signals can include, e.g., the ATG initiation codon and adjacent sequences. In cases where a coding sequence, its initiation codon and upstream sequences are inserted into the appropriate expression vector, no additional translational control signals may be needed. However, in cases where only coding sequence (e.g., a mature protein coding sequence), or a portion thereof, is inserted, exogenous transcriptional control signals including the ATG initiation codon can be separately provided. The initiation codon is provided in the correct reading frame to facilitate transcription. Exogenous transcriptional elements and initiation codons can be of various origins, both natural and synthetic. The efficiency of expression can be enhanced by the inclusion of enhancers appropriate to the cell system in use. Expression Hosts
  • the present invention also relates to host cells which are transduced with vectors of the invention, and the production of polypeptides of the invention (including fragments thereof) by recombinant techniques.
  • Host cells are genetically engineered (i.e, nucleic acids are introduced, e.g., transduced, transformed or transfected) with the vectors of this invention, which may be, for example, a cloning vector or an expression vector comprising the relevant nucleic acids herein.
  • the vector is optionally a plasmid, a viral particle, a phage, a naked nucleic acids, etc.
  • the engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants, or amplifying the relevant gene.
  • the culture conditions are those previously used with the host cell selected for expression, and will be apparent to those skilled in the art and in the references cited herein, including, Sambrook and Ausubel.
  • the host cell can be a eukaryotic cell, such as a yeast cell, or a plant cell, or the host cell can be a prokaryotic cell, such as a bacterial cell.
  • Plant protoplasts are also suitable for some applications.
  • the DNA fragments are introduced into plant tissues, cultured plant cells or plant protoplasts by standard methods including electroporation (Fromm et al., (1985) Proc. Natl. Acad. Sci.
  • the T-DNA plasmid is transmitted to plant cells upon infection by Agrobacterium tumefaciens, and a portion is stably integrated into the plant genome (Horsch et al. (1984) Science 233:496-498: Fraley et al. (1983) Proc. Natl. Acad. Sci. USA 80, 4803).
  • the cell can include a nucleic acid of the invention which encodes a polypeptide, wherein the cells expresses a polypeptide of the invention.
  • the cell can also include vector sequences, or the like.
  • cells and transgenic plants which include any polypeptide or nucleic acid above or throughout this specification, e.g., produced by transduction of a vector of the invention, are an additional feature of the invention.
  • Host cells transformed with a nucleotide sequence encoding a polypeptide of the invention are optionally cultured under conditions suitable for the expression and recovery of the encoded protein from cell culture.
  • the protein or fragment thereof produced by a recombinant cell may be secreted, membrane-bound, or contained intracellularly, depending on the sequence and/or the vector used.
  • expression vectors containing polynucleotides encoding mature proteins of the invention can be designed with signal sequences which direct secretion of the mature polypeptides through a prokaryotic or eukaryotic cell membrane.
  • a transcription factor provided by the present invention can also be used to identify additional endogenous or exogenous molecules that can affect a phentoype or trait of interest.
  • such molecules include organic (small or large molecules) and/or inorganic compounds that affect expression of (i.e., regulate) a particular transcription factor.
  • such molecules include endogenous molecules that are acted upon either at a transcriptional level by a transcription factor of the invention to modify a phenotype as desired.
  • the transcription factors can be employed to identify one or more downstream gene with which is subject to a regulatory effect of the transcription factor.
  • a transcription factor or transcription factor homologue of the invention is expressed in a host cell, e.g, a transgenic plant cell, tissue or explant, and expression products, either RNA or protein, of likely or random targets are monitored, e.g., by hybridization to a microarray of nucleic acid probes corresponding to genes expressed in a tissue or cell type of interest, by two-dimensional gel electrophoresis of protein products, or by any other method known in the art for assessing expression of gene products at the level of RNA or protein.
  • a transcription factor of the invention can be used to identify promoter sequences (i.e., binding sites) involved in the regulation of a downstream target.
  • interactions between the transcription factor and the promoter sequence can be modified by changing specific nucleotides in the promoter sequence or specific amino acids in the transcription factor that interact with the promoter sequence to alter a plant trait.
  • transcription factor DNA binding sites are identified by gel shift assays.
  • the promoter region sequences can be employed in double-stranded DNA arrays to identify molecules that affect the interactions of the transcription factors with their promoters (Bulyk et al. (1999) Nature Biotechnology 17:573-577).
  • the identified transcription factors are also useful to identify proteins that modify the activity of the transcription factor.
  • Such modification can occur by covalent modification, such as by phosphorylation, or by protein-protein (homo or- heteropolymer) interactions. Any method suitable for detecting protein-protein interactions can be employed. Among the methods that can be employed are co- immunoprecipitation, cross-linking and co-purification through gradients or chromatographic columns, and the two-hybrid yeast system.
  • the two-hybrid system detects protein interactions in vivo and is described in Chien, et al., (1991), Proc. Natl. Acad. Sci. USA 88, 9578-9582 and is commercially available from Clontech (Palo Alto, Calif).
  • plasmids are constmcted that encode two hybrid proteins: one consists of the DNA-binding domain of a transcription activator protein fused to the TF polypeptide and the other consists of the transcription activator protein's activation domain fused to an unknown protein that is encoded by a cDNA that has been recombined into the plasmid as part of a cDNA library.
  • the DNA-binding domain fusion plasmid and the cDNA library are transfomied into a strain of the yeast Saccharomyces cerevisiae that contains a reporter gene (e.g., lacZ) whose regulatory region contains the transcription activator's binding site. Either hybrid protein alone cannot activate transcription of the reporter gene. Interaction of the two hybrid proteins reconstitutes the functional activator protein and results in expression of the reporter gene, which is detected by an assay for the reporter gene product. Then, the library plasmids responsible for reporter gene expression are isolated and sequenced to identify the proteins encoded by the library plasmids. After identifying proteins that interact with the transcription factors, assays for compounds that interfere with the TF protein-protein interactions can be preformed.
  • a reporter gene e.g., lacZ
  • extracellular molecules that alter activity or expression of a transcription factor can be identified.
  • the methods can entail first placing a candidate molecule in contact with a plant or plant cell.
  • the molecule can be introduced by topical administration, such as spraying or soaking of a plant, and then the molecule's effect on the expression or activity of the TF polypeptide or the expression of the polynucleotide monitored. Changes in the expression of the TF polypeptide can be monitored by use of polyclonal or monoclonal antibodies, gel electrophoresis or the like.
  • Changes in the expression of the corresponding polynucleotide sequence can be detected by use of microarrays, Northerns, quantitative PCR, or any other technique for monitoring changes in mRNA expression. These techniques are exemplified in Ausubel et al. (eds) Current
  • any available composition can be tested for modulatory activity of expression or activity of any nucleic acid or polypeptide herein.
  • available libraries of compounds such as chemicals, polypeptides, nucleic acids and the like can be tested for modulatory activity.
  • potential modulator compounds can be dissolved in aqueous or organic (e.g., DMSO-based) solutions for easy delivery to the cell or plant of interest in which the activity of the modulator is to be tested.
  • the assays are designed to screen large modulator composition libraries by automating the assay steps and providing compounds from any convenient source to assays, which are typically mn in parallel (e.g., in microtiter formats on microtiter plates in robotic assays).
  • high throughput screening methods involve providing a combinatorial library containing a large number of potential compounds (potential modulator compounds).
  • Such "combinatorial chemical libraries” are then screened in one or more assays, as described herein, to identify those library members (particular chemical species or subclasses) that display a desired characteristic activity. The compounds thus identified can serve as target compounds.
  • a combinatorial chemical library can be, e.g., a collection of diverse chemical compounds generated by chemical synthesis or biological synthesis.
  • a combinatorial chemical library such as a polypeptide library is formed by combining a set of chemical building blocks (e.g., in one example, amino acids) in every possible way for a given compound length (i.e., the number of amino acids in a polypeptide compound of a set length).
  • Exemplary libraries include peptide libraries, nucleic acid libraries, antibody libraries (see, e.g., Vaughn et al. (1996) Nature Biotechnology, 14(3):309-314 and PCT/US96/10287), carbohydrate libraries (see, e.g., Liang et al.
  • combinatorial or other libraries are well known to those of skill in the art.
  • Such combinatorial chemical libraries include, but are not limited to, peptide libraries (see, e.g., U.S. Patent 5,010,175, Furka, Int. J. Pept. Prot. Res. 37:487-493 (1991) and Houghton et al. Nature 354:84-88 (1991)).
  • Other chemistries for generating chemical diversity libraries can also be used.
  • compound screening equipment for high- throughput screening is generally available, e.g., using any of a number of well known robotic systems that have also been developed for solution phase chemistries useful in assay systems.
  • Zymark Co ⁇ provides technical bulletins describing screening systems for detecting the modulation of gene transcription, ligand binding, and the like.
  • the integrated systems herein in addition to providing for sequence alignment and, optionally, synthesis of relevant nucleic acids, can include such screening apparatus to identify modulators that have an effect on one or more polynucleotides or polypeptides according to the present invention.
  • positive controls it is desirable to have positive controls to ensure that the components of the assays are working properly. At least two types of positive controls are appropriate. That is, known transcriptional activators or inhibitors can be incubated with cells/plants/ etc.
  • modulators can also be combined with transcriptional activators or inhibitors to find modulators which inhibit transcriptional activation or transcriptional repression. Either expression of the nucleic acids and proteins herein or any additional nucleic acids or proteins activated by the nucleic acids or proteins herein, or both, can be monitored.
  • the invention provides a method for identifying compositions that modulate the activity or expression of a polynucleotide or polypeptide of the invention.
  • a test compound whether a small or large molecule, is placed in contact with a cell, plant (or plant tissue or explant), or composition comprising the polynucleotide or polypeptide of interest and a resulting effect on the cell, plant, (or tissue or explant) or composition is evaluated by monitoring, either directly or indirectly, one or more of: expression level of the polynucleotide or polypeptide, activity (or modulation of the activity) of the polynucleotide or polypeptide.
  • an alteration in a plant phenotype can be detected following contact of a plant (or plant cell, or tissue or explant) with the putative modulator, e.g., by modulation of expression or activity of a polynucleotide or polypeptide of the invention.
  • polynucleotides also referred to herein as oligonucleotides, typically having at least 12 bases, preferably at least 15, more preferably at least 20, 30, or 50 bases, which hybridize under at least highly stringent (or ultra-high stringent or ultra-ultra- high stringent conditions) conditions to a polynucleotide sequence described above.
  • the polynucleotides may be used as probes, primers, sense and antisense agents, and the like, according to methods as noted supra.
  • Subsequences of the polynucleotides of the invention, including polynucleotide fragments and oligonucleotides are useful as nucleic acid probes and primers.
  • An oligonucleotide suitable for use as a probe or primer is at least about 15 nucleotides in length, more often at least about 18 nucleotides, often at least about 21 nucleotides, frequently at least about 30 nucleotides, or about 40 nucleotides, or more in length.
  • a nucleic acid probe is useful in hybridization protocols, e.g., to identify additional polypeptide homologues of the invention, including protocols for microarray experiments.
  • Primers can be annealed to a complementary target DNA strand by nucleic acid hybridization to form a hybrid between the primer and the target DNA strand, and then extended along the target DNA strand by a DNA polymerase enzyme. Primer pairs can be used for amplification of a nucleic acid sequence, e.g., by the polymerase chain reaction (PCR) or other nucleic-acid amplification methods. See Sambrook and Ausubel, supra.
  • PCR polymerase chain reaction
  • the invention includes an isolated or recombinant polypeptide including a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotides of the invention.
  • polypeptides, or domains or fragments thereof can be used as immunogens, e.g., to produce antibodies specific for the polypeptide sequence, or as probes for detecting a sequence of interest.
  • a subsequence can range in size from about 15 amino acids in length up to and including the full length of the polypeptide.
  • the polynucleotides of the invention are favorably employed to produce transgenic plants with various traits, or characteristics, that have been modified in a desirable manner, e.g., to improve the pathogen resistance of a plant.
  • alteration of expression levels or patterns e.g., spatial or temporal expression patterns
  • of one or more of the transcription factors (or transcription factor homologues) of the invention as compared with the levels of the same protein found in a wild type plant, can be used to modify a plant's traits.
  • An illustrative example of trait modification, improved flowering phenotype, by altering expression levels of a particular transcription factor is described further in the Examples and the Sequence Listing. Antisense and Cosuppression Approaches
  • nucleic acids of the invention are also useful for sense and anti-sense suppression of expression, e.g., to down-regulate expression of a nucleic acid of the invention, e.g., as a further mechanism for modulating plant phenotype. That is, the nucleic acids of the invention, or subsequences or anti-sense sequences thereof, can be used to block expression of naturally occurring homologous nucleic acids.
  • sense and anti-sense technologies are known in the art, e.g., as set forth in Lichtenstein and Nellen (1997) Antisense Technology: A Practical Approach IRL Press at Oxford University, Oxford, England.
  • sense or anti-sense sequences are introduced into a cell, where they are optionally amplified, e.g., by transcription.
  • Such sequences include both simple oligonucleotide sequences and catalytic sequences such as ribozymes.
  • a reduction or elimination of expression i.e., a "knockout" of a transcription factor or transcription factor homologue polypeptide in a transgenic plant, e.g., to modify a plant trait
  • an antisense construct corresponding to the polypeptide of interest as a cDNA.
  • the transcription factor or homologue cDNA is arranged in reverse orientation (with respect to the coding sequence) relative to the promoter sequence in the expression vector.
  • the introduced sequence need not be the full length cDNA or gene, and need not be identical to the cDNA or gene found in the plant type to be transformed.
  • the antisense sequence need only be capable of hybridizing to the target gene or RNA of interest.
  • the introduced sequence is of shorter length, a higher degree of homology to the endogenous transcription factor sequence will be needed for effective antisense suppression.
  • antisense sequences of various lengths can be utilized, preferably, the introduced antisense sequence in the vector will be at least 30 nucleotides in length, and improved antisense suppression will typically be observed as the length of the antisense sequence increases.
  • the length of the antisense sequence in the vector will be greater than 100 nucleotides. Transcription of an antisense construct as described results in the production of RNA molecules that are the reverse complement of mRNA molecules transcribed from the endogenous transcription factor gene in the plant cell.
  • Ribozymes are RNA molecules that possess highly specific endoribonuclease activity. The production and use of ribozymes are disclosed in U.S. Patent No. 4,987,071 and U.S. Patent No. 5,543,508. Synthetic ribozyme sequences including antisense RNAs can be used to confer RNA cleaving activity on the antisense RNA, such that endogenous mRNA molecules that hybridize to the antisense RNA are cleaved, which in rum leads to an enhanced antisense inhibition of endogenous gene expression.
  • RNA encoded by a transcription factor or transcription factor homologue cDNA can also be used to obtain co- suppression of a corresponding endogenous gene, e.g., in the manner described in U.S. Patent No. 5,231,020 to Jorgensen.
  • Such co-suppression also termed sense suppression
  • the suppressive efficiency will be enhanced as specificity of hybridization is increased, e.g., as the introduced sequence is lengthened, and/or as the sequence similarity between the introduced sequence and the endogenous transcription factor gene is increased.
  • Vectors expressing an untranslatable form of the transcription factor mRNA can also be used to suppress expression of an endogenous transcription factor, thereby reducing or eliminating it's activity and modifying one or more traits.
  • Methods for producing such constructs are described in U.S. Patent No. 5,583,021.
  • constmcts are made by introducing a premature stop codon into the transcription factor gene.
  • a plant trait can be modified by gene silencing using double-strand RNA (Sha ⁇ (1999) Genes and Development 13: 139-141).
  • Another method for abolishing the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens. After generating the insertion mutants, the mutants can be screened to identify those containing the insertion in a transcription factor or transcription factor homologue gene. Plants containing a single transgene insertion event at the desired gene can be crossed to generate homozygous plants for the mutation (Koncz et al. (1992) Methods in Arabidopsis Research, World Scientific).
  • a plant phenotype can be altered by eliminating an endogenous gene, such as a transcription factor or transcription factor homologue, e.g., by homologous recombination (Kempin et al. (1997) Nature 389:802).
  • a plant trait can also be modified by using the cre-lox system (for example, as described in US Pat. No. 5,658,772).
  • a plant genome can be modified to include first and second lox sites that are then contacted with a Cre recombinase. If the lox sites are in the same orientation, the intervening DNA sequence between the two sites is excised. If the lox sites are in the opposite orientation, the intervening sequence is inverted.
  • polynucleotides and polypeptides of this invention can also be expressed in a plant in the absence of an expression cassette by manipulating the activity or expression level of the endogenous gene by other means.
  • T-DNA activation tagging Ichikawa et al. (1997) Nature 390 698-701 ; Kakimoto et al. (1996) Science 274: 982-985.
  • This method entails transforming a plant with a gene tag containing multiple transcriptional enhancers and once the tag has inserted into the genome, expression of a flanking gene coding sequence becomes deregulated.
  • the transcriptional machinery in a plant can be modified so as to increase transcription levels of a polynucleotide of the invention (See, e.g., PCT Publications WO 96/06166 and WO 98/53057 which describe the modification of the DNA binding specificity of zinc finger proteins by changing particular amino acids in the DNA binding motif).
  • the transgenic plant can also include the cellular machinery or mechanisms necessary for expressing or altering the activity of a polypeptide encoded by an endogenous gene, for example by altering the phosphorylation state of the polypeptide to maintain it in an activated state.
  • Transgenic plants inco ⁇ orating the polynucleotides of the invention and/or expressing the polypeptides of the invention can be produced by a variety of well established techniques as described above.
  • an expression cassette including a polynucleotide, e.g., encoding a transcription factor or transcription factor homologue, of the invention
  • standard techniques can be used to introduce the polynucleotide into a plant, a plant cell, a plant explant or a plant tissue of interest.
  • the plant cell, explant or tissue can be regenerated to produce a transgenic plant.
  • the plant can be any higher plant, including gymnosperms, monocotyledonous and dicotyledenous plants. Suitable protocols are available for Leguminosae (alfalfa, soybean, clover, etc.), Umbelliferae (carrot, celery, parsnip), Cruciferae (cabbage, radish, rapeseed, broccoli, etc.), Curcurhitaceae (melons and cucumber), Gramineae (wheat, com, rice, barley, millet, etc.), Solanaceae (potato, tomato, tobacco, peppers, etc.), and various other crops. See protocols described in Ammirato et al. (1984) Handbook of Plant Cell Culture -Crop Species. Macmillan Publ.
  • Suitable methods can include, but are not limited to: electroporation of plant protoplasts; liposome- mediated transformation; polyethylene glycol (PEG) mediated transformation; transformation using viruses; micro-injection of plant cells; micro-projectile bombardment of plant cells; vacuum infiltration; and Agrobacterium tumeficiens mediated transformation. Transformation means introducing a nucleotide sequence in a plant in a manner to cause stable or transient expression of the sequence.
  • modified traits can be any of those traits described above. Additionally, to confirm that the modified trait is due to changes in expression levels or activity of the polypeptide or polynucleotide of the invention can be determined by analyzing mRNA expression using Northern blots, RT-PCR or microarrays, or protein expression using immunoblots or Western blots or gel shift assays.
  • the present invention may be an integrated system, computer or computer readable medium that comprises an instruction set for determining the identity of one or more sequences in a database.
  • the instruction set can be used to generate or identify sequences that meet any specified criteria.
  • the instruction set may be used to associate or link certain functional benefits, such improved flowering phenotype, with one or more identified sequence.
  • the instruction set can include, e.g., a sequence comparison or other alignment program, e.g., an available program such as, for example, the Wisconsin Package Version 10.0, such as BLAST, FASTA, PILEUP, FINDPATTERNS or the like (GCG, Madison, WI).
  • a sequence comparison or other alignment program e.g., an available program such as, for example, the Wisconsin Package Version 10.0, such as BLAST, FASTA, PILEUP, FINDPATTERNS or the like (GCG, Madison, WI).
  • GCG Madison, WI
  • Public sequence databases such as GenBank, EMBL, Swiss-Prot and PIR or private sequence databases such as PhytoSeq (Incyte Pharmaceuticals, Palo Alto, CA) can be searched.
  • Alignment of sequences for comparison can be conducted by the local homology algorithm of Smith and Waterman (1981) Adv. Appl. Math. 2:482, by the homology alignment algorithm of Needleman and Wunsch (1970) J. Mol. Biol. 48:443, by the search for similarity method of Pearson and Lipman (1988) Proc. Natl. Acad. Sci. U.S.A. 85: 2444, by computerized implementations of these algorithms.
  • sequence comparisons between two (or more) polynucleotides or polypeptides are typically performed by comparing sequences of the two sequences over a comparison window to identify and compare local regions of sequence similarity.
  • the comparison window can be a segment of at least about 20 contiguous positions, usually about 50 to about 200, more usually about 100 to about 150 contiguous positions.
  • a description of the method is provided in Ausubel et al., supra.
  • a variety of methods of determining sequence relationships can be used, including manual alignment and computer assisted sequence alignment and analysis. This later approach is a preferred approach in the present invention, due to the increased throughput afforded by computer assisted methods.
  • a variety of computer programs for performing sequence alignment are available, or can be produced by one of skill.
  • HSPs high scoring sequence pairs
  • initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them.
  • the word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always > 0) and N (penalty score for mismatching residues; always ⁇ 0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached.
  • the BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment.
  • the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff (1989) Proc. Natl. Acad. Sci. USA 89: 10915).
  • the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g..
  • P(N) the smallest sum probability
  • P(N) the smallest sum probability
  • a nucleic acid is considered similar to a reference sequence (and, therefore, in this context, homologous) if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.1, or less than about 0.01 , and or even less than about 0.001.
  • PILEUP An additional example of a useful sequence alignment algorithm is PILEUP.
  • PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pairwise alignments.
  • the program can align, e.g., up to 300 sequences of a maximum length of 5,000 letters.
  • the integrated system, or computer typically includes a user input interface allowing a user to selectively view one or more sequence records corresponding to the one or more character strings, as well as an instruction set which aligns the one or more character strings with each other or with an additional character string to identify one or more region of sequence similarity.
  • the system may include a link of one or more character strings with a particular phenotype or gene function.
  • the system includes a user readable output element which displays an alignment produced by the alignment instruction set.
  • the methods of this invention can be implemented in a localized or distributed computing environment.
  • the methods may implemented on a single computer comprising multiple processors or on a multiplicity of computers.
  • the computers can be linked, e.g. through a common bus, but more preferably the computer(s) are nodes on a network.
  • the network can be a generalized or a dedicated local or wide-area network and, in certain preferred embodiments, the computers may be components of an intra-net or an internet.
  • the invention provides methods for identifying a sequence similar or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence.
  • a sequence database is provided (locally or across an inter or intra net) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.
  • Any sequence herein can be entered into the database, before or after querying the database. This provides for both expansion of the database and, if done before the querying step, for insertion of control sequences into the database.
  • the control sequences can be detected by the query to ensure the general integrity of both the database and the query.
  • the query can be performed using a web browser based interface.
  • the database can be a centralized public database such as those noted herein, and the querying can be done from a remote terminal or computer across an internet or intranet.
  • Putative transcription factor sequences (genomic or ESTs) related to known transcription factors were identified in the Arabidopsis thaliana GenBank database using the tblastn sequence analysis program using default parameters and a P-value cutoff threshold of -4 or -5 or lower, depending on the length of the query sequence. Putative transcription factor sequence hits were then screened to identify those containing particular sequence strings. If the sequence hits contained such sequence strings, the sequences were confirmed as transcription factors.
  • Arabidopsis thaliana cDNA libraries derived from different tissues or treatments, or genomic libraries were screened to identify novel members of a transcription family using a low stringency hybridization approach. Probes were synthesized using gene specific primers in a standard PCR reaction
  • RACE 5' and 3' rapid amplification of cDNA ends
  • Gene-specific primers were designed to be used along with adaptor specific primers for both 5' and 3' RACE reactions. Nested primers, rather than single primers, were used to increase PCR specificity. Using 5' and 3' RACE reactions, 5' and 3 ' RACE fragments were obtained, sequenced and cloned. The process can be repeated until 5' and 3' ends of the full-length gene were identified. Then the full- length cDNA was generated by PCR using primers specific to 5' and 3' ends of the gene by end-to-end PCR.
  • the sequence was amplified from a genomic or cDNA library using primers specific to sequences upstream and downstream of the coding region.
  • the expression vector was pMEN20 or pMEN65, which are both derived from pMON316 (Sanders et al, (1987) Nucleic Acids Research 15:1543-58) and contain the CaMV 35S promoter to express transgenes.
  • pMEN20 and pMEN65 were digested separately with Sail and Notl restriction enzymes at 37° C for 2 hours. The digestion products were subject to electrophoresis in a 0.8% agarose gel and visualized by ethidium bromide staining.
  • the DNA fragments containing the sequence and the linearized plasmid were excised and purified by using a Qiaquick gel extraction kit (Qiagen, CA).
  • the fragments of interest were ligated at a ratio of 3: 1 (vector to insert).
  • Ligation reactions using T4 DNA ligase (New England Biolabs, MA) were carried out at 16° C for 16 hours.
  • the ligated DNAs were transformed into competent cells of the E. coli strain DH5alpha by using the heat shock method.
  • the transformations were plated on LB plates containing 50 mg/1 kanamycin (Sigma). Individual colonies were grown overnight in five milliliters of LB broth containing 50 mg/1 kanamycin at 37° C. Plasmid DNA was purified by using Qiaquick Mini Prep kits (Qiagen, CA).
  • the vector was used to transform Agrobacterium tumefaciens cells expressing the gene products.
  • the stock of Agrobacterium tumefaciens cells for transformation were made as described by Nagel et al. (1990) FEMS Microbiol Letts. 67: 325-328.
  • Agrobacterium strain ABI was grown in 250 ml LB medium (Sigma) overnight at 28°C with shaking until an absorbance (A 6 oo) of 0.5 - 1.0 was reached. Cells were harvested by centrifugation at 4,000 x g for 15 min at 4° C.
  • Agrobacterium cells were transformed with plasmids prepared as described above following the protocol described by Nagel et al.
  • 50 - 100 ng DNA (generally resuspended in 10 mM Tris- HCl, 1 mM EDTA, pH 8.0) was mixed with 40 ⁇ l of Agrobacterium cells.
  • the DNA/cell mixture was then transferred to a chilled cuvette with a 2mm electrode gap and subject to a 2.5 kV charge dissipated at 25 ⁇ F and 200 ⁇ F using a Gene Pulser II apparatus (Bio-Rad).
  • cells were immediately resuspended in 1.0 ml LB and allowed to recover without antibiotic selection for 2 - 4 hours at 28° C in a shaking incubator. After recovery, cells were plated onto selective medium of LB broth containing 100 ⁇ g/ml spectinomycin (Sigma) and incubated for 24-48 hours at 28° C. Single colonies were then picked and inoculated in fresh medium. The presence of the plasmid construct was verified by PCR amplification and sequence analysis.
  • Agrobacterium tumefaciens After transformation of Agrobacterium tumefaciens with plasmid vectors containing the gene, single Agrobacterium colonies were identified, propagated, and used to transform Arabidopsis plants. Briefly, 500 ml cultures of LB medium containing 50 mg/1 kanamycin were inoculated with the colonies and grown at 28° C with shaking for 2 days until an absorbance (A ⁇ oo) of > 2.0 is reached.
  • Cells were then harvested by centrifugation at 4,000 x g for 10 min, and resuspended in infiltration medium (1/2 X Murashige and Skoog salts (Sigma), 1 X Gamborg's B-5 vitamins (Sigma), 5.0% (w/v) sucrose (Sigma), 0.044 ⁇ M benzylamino purine (Sigma), 200 ⁇ l/L Silwet L-77 (Lehle Seeds) until an absorbance (A 60 o) of 0.8 was reached.
  • infiltration medium 1/2 X Murashige and Skoog salts (Sigma), 1 X Gamborg's B-5 vitamins (Sigma), 5.0% (w/v) sucrose (Sigma), 0.044 ⁇ M benzylamino purine (Sigma), 200 ⁇ l/L Silwet L-77 (Lehle Seeds) until an absorbance (A 60 o) of 0.8 was reached.
  • Arabidopsis thaliana seeds Prior to transformation, Arabidopsis thaliana seeds (ecotype Columbia) were sown at a density of -10 plants per 4" pot onto Pro-Mix BX potting medium (Hummert International) covered with fiberglass mesh (18 mm X 16 mm). Plants were grown under continuous illumination (50-75 ⁇ E/m 2 /sec) at 22-23° C with 65-70% relative humidity. After about 4 weeks, primary inflorescence stems (bolts) are cut off to encourage growth of multiple secondary bolts. After flowering of the mature secondary bolts, plants were prepared for transformation by removal of all siliques and opened flowers.
  • the pots were then immersed upside down in the mixture of Agrobacterium infiltration medium as described above for 30 sec, and placed on their sides to allow draining into a 1 ' x 2' flat surface covered with plastic wrap. After 24 h, the plastic wrap was removed and pots are turned upright. The immersion procedure was repeated one week later, for a total of two immersions per pot. Seeds were then collected from each transformation pot and analyzed following the protocol described below.
  • Seeds collected from the transformation pots were sterilized essentially as follows. Seeds were dispersed into in a solution containing 0.1% (v/v) Triton X- 100 (Sigma) and sterile H 0 and washed by shaking the suspension for 20 min. The wash solution was then drained and replaced with fresh wash solution to wash the seeds for 20 min with shaking. After removal of the second wash solution, a solution containing 0.1% (v/v) Triton X-100 and 70% ethanol (Equistar) was added to the seeds and the suspension was shaken for 5 min.
  • a solution containing 0.1% (v/v) Triton X-100 and 70% ethanol (Equistar) was added to the seeds and the suspension was shaken for 5 min.
  • a solution containing 0.1% (v/v) Triton X-100 and 30% (v/v) bleach (Clorox) was added to the seeds, and the suspension was shaken for 10 min. After removal of the bleach/detergent solution, seeds were then washed five times in sterile distilled H 2 0. The seeds were stored in the last wash water at 4° C for 2 days in the dark before being plated onto antibiotic selection medium (1 X Murashige and Skoog salts (pH adjusted to 5.7 with IM KOH), 1 X Gamborg's B-5 vitamins, 0.9% phytagar (Life Technologies), and 50 mg/1 kanamycin).
  • antibiotic selection medium (1 X Murashige and Skoog salts (pH adjusted to 5.7 with IM KOH), 1 X Gamborg's B-5 vitamins, 0.9% phytagar (Life Technologies), and 50 mg/1 kanamycin).
  • Seeds were germinated under continuous illumination (50-75 ⁇ E/m 2 /sec) at 22-23° C. After 7-10 days of growth under these conditions, kanamycin resistant primary transformants (Ti generation) were visible and obtained. These seedlings were transferred first to fresh selection plates where the seedlings continued to grow for 3-5 more days, and then to soil (Pro-Mix BX potting medium).
  • plants overexpressing G2010 constitutively (three independent T2 populations having 6 plants in one population and 16 plants in each of the other two) flowered approximately 1 week earlier than control plants transformed with an empty transformation vector under the control of the 35S promoter.
  • the primary shoot of 35S::G2010 plants produced 5-6 rosette leaves before bolting, compared to 8-10 rosette leaves in controls.
  • Flower buds were first visible 12-14 days after sowing in 35S::G2010 plants compared with approximately 20 days for wild type. At 20 days the 35S::G2010 plants have open flowers at this time whereas the wild type has yet to generate an inflorescence.
  • G2010 is a member of the SBP family of transcription factors and corresponds to spl4 (Cardon et al. (1999) Gene 237:91-104). Expression of spl4 is upregulated during development under both long day and short day conditions and spl4 is highly expressed in the inflorescence tissue. Expression of G2010 is localized to the rib meristem and inter-primordial regions of the inflorescence apex (Cardon et al. (1999) Gene 237:91-104).
  • G2010 The utility of a gene such as G2010 that functions to accelerate flowering includes improving modem crop varieties, for example. Most modern crop varieties are the result of extensive breeding programs. Many generations of backcrossing may be required to introduce desired traits. Systems that accelerate flowering could have valuable applications in such programs since they allow much faster generation times. Additionally, in some instances, a faster generation time might allow additional harvests of a crop to be made within a given growing season.
  • G2347 SEQ ID NO: 19 and 20. G2347 shares about 52% sequence identity over the whole sequence length and 95% sequence identity over the conserved domain.
  • Knockout G1037 plants (12 individuals in total), grown under continuous light conditions at 20-25° C, produced 4-7 primary rosette leaves before bolting compared to 8-9 rosette leaves in controls harboring an empty transformation vector. Flower buds were first visible in knockout G1037 plants approximately 1 week earlier than in controls. Early flowering was also noted in knockout G 1037 plants grown for 1 week in continuous light followed by subsequent growth under 12 hours light. At 28 days the flower buds are visible in the knockout G1037 but not the controls.
  • G722 (SEQ ID NO: 21 and 22) and G1493 (SEQ ID NO: 23 and 24).
  • G722 shares about 66% sequence identity over the whole sequence length compared with G1037 and 78% sequence identity over the conserved domain compared with G1037.
  • G1493 shares about 40% sequence identity over the whole sequence length and 78% sequence identity over the conserved domain.
  • additional overexpressor plants that also had a modified flowering phenotype. Table 5 shows the phenotypes observed for particular overexpressor or knockout plants and provides the SEQ ID No., the internal reference code (GID), whether a knockout or overexpressor plant was analyzed and the observed phenotype.
  • GID internal reference code
  • Gl 52 (SEQ ID NO: 13 and 14), G153 (SEQ ID NO: 15 and 16), and G860 (SEQ ID NO: 17 and 18) were all found to be related to G1760 (SEQ ID NO: 3 and 4).
  • Gl 52 shares about 75% sequence identity over the whole sequence length and 93% sequence identity over the conserved domain.
  • G153 shares about 60% sequence identity over the whole sequence length and 85% sequence identity over the conserved domain.
  • G860 shares about 61% sequence identity over the whole sequence length and 85% sequence identity over the conserved domain.
  • Another knockout G1947 (SEQ ID NO: 11 and 12) showed an extended flowering period or extended reproductive phase.
  • a control plant population produced flowers for approximately 15 days whereas the overexpressor plant population flowered for approximately 30 days due to a longer retention period of the flowers or a delay in senescense.
  • a variety of plant traits can be altered. For example, plants with accelerated, delayed, or inducible flowering times may be generated. Alternatively the vernalization period or flower retention period or an increase in the total number of flowers may be achieved.
  • a number of Arabidopsis genes have already been shown to accelerate flowering when constitutively expressed. These include LEAFY, APETALA1 and CONSTANS. In these cases, however, the early flowering plants showed undesirable side effects such as extreme dwarfing, infertility, or premature termination of shoot meristem growth (Mandel, M. et al., 1995, Nature 377, 522-524; Weigel, D. and Nilsson, O., 1995, Nature 377, 495-500; Simon et al., 1996, Nature 384, 59-62, Onouchi et al., 2000, Plant Cell 12, 885-900). Systems that accelerate flowering could have valuable applications in modem crop production programs since they allow much faster generation times. Additionally, in some instances, a faster generation time might allow additional harvests of a crop to be made within a given growing season.
  • inducible promoters By regulating the expression of genes of the invention in transgenic plants using inducible promoters, flowering could be triggered by application of an inducer chemical. This would allow flowering to be synchronized across a crop and facilitate more efficient harvesting.
  • Such inducible systems could be used to tune the flowering of crop varieties to different latitudes.
  • species such as soybean and cotton are available as a series of maturity groups that are suitable for different latitudes on the basis of their flowering time (which is governed by day-length).
  • a system in which flowering could be chemically controlled would allow a single high-yielding Northern maturity group to be grown at any latitude. In Southern regions such plants could be grown for longer, thereby increasing yields, before flowering was induced. In more Northern areas, the induction would be used to ensure that the crop flowers prior to the first winter frosts.
  • Genes or sequences selected from Table 1 or Table 2 can also be overexpressed or knocked out in a plant to produce a plant with modified flowering trait.
  • the sequence selected (Test Sequence) from Table 1 or Table 2 is overexpressed in the same species listed for the selected sequences, however, another species may be used.
  • Arabidopsis are identified using database sequence search tools, such as the Basic Local Alignment Search Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215:403- 410; and Altschul et al. (1997) Nucl. Acid Res. 25: 3389-3402).
  • the tblastx sequence analysis programs are employed using the BLOSUM-62 scoring matrix (Henikoff, S. and Henikoff, J. G. (1992) Proc. Natl. Acad. Sci. USA 89: 10915-10919).
  • Identified Arabidopsis homologous sequences are provided in Tables 1 and 2, appended to this application. These sequences can be inserted into a plant to modify a flowering trait as shown above.
  • the percent sequence identity among these sequences can be as low as 47%, or even lower sequence identity.
  • the entire NCBI GenBank database was filtered for sequences from all plants except Arabidopsis thaliana by selecting all entries in the NCBI GenBank database associated with NCBI taxonomic ID 33090 (Viridiplantae; all plants) and excluding entries associated with taxonomic ID 3701 (Arabidopsis thaliana).
  • Table 1 (appended to this application) lists a summary of orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search.
  • the first column shows the polynucleotide sequence identifier (SEQ ID NO)
  • the second column shows the transcription factor cDNA identifier (Gene ID)
  • the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide sequence identified in a BLAST search (Test Sequence ID)
  • the fourth column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability)
  • the fifth column identifies the plant species of the Test Sequence (Test Sequence Species)
  • the sixth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation).
  • Table 2 (appended to this application) lists orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search.
  • the first column shows the polynucleotide sequence identifier (SEQ ID NO)
  • the second column shows the transcription factor cDNA identifier (Gene ID)
  • the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide (Test Sequence ID)
  • the fourth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation)
  • the fifth column shows the reading frame of the Test sequence encoding the orthologous or homologous sequence (Reading Frame)
  • the sixth column shows the calculated score value of the aligned sequences (High Score)
  • the seventh column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability)
  • the eighth column shows the number of regions in the Test Sequence that align with a sequence
  • G1820 gi1087017 1 [Nicotiana alata] arabinogalactan-protein, AGP [Nicotiana a
  • G2010 gi9087308 7.40E-10 Mitochondrion Beta vulgaris var. altiss orf102a.
  • BJ 181458 4.10E-34 [Physcomitrella patens subsp. patens] BJ 181458 normalized ful
  • G590 GI-8468039 Similar to Arabidopsis thaliana chromosome... -1 73 0.045 3
  • GHDEL61 Gossypium hirsutum] -2 67 0.97 2
  • G1760 GI-5295964 EST D15657(C1032) corresponds to a region... 207 3.50E-27 4
  • G1760 GI-3913005 AG_PANGI AGAMOUS PROTEIN (GAG2) 209 1.20E-26 3
  • G1760 GI-4103342 agamous-like putative transcription factor 212 6.10E-26 3
  • G1760 GI-4101710 MADS box transcription factor 217 8.10E-25 3
  • G1760 GI-4103344 agamous-like putative transcription factor 210 8.50E-25 3
  • G1760 GI-4103486 MADS box protein 217 1.OOE-24 3
  • G1760 GI-939779 MADS box protein 213 1.70E-24 3

Abstract

Recombinant polynucleotides and methods for modifying the phenotype of a plant are provided: In preferred embodiments, the phenotype modified is a plant's flowering time or flowering period. Increasing or reducing flowering time or the flowering period or modifying the number of flowers generated leads to commercially superior crops and plants. The method comprises altering the levels of a transcription factor that is introduced into the plant or that is endogenous to the plant.

Description

METHODS FOR MODIFYING FLOWERING PHENOTYPES
This application claims priority to U.S. Patent Application Serial No. 09/819,142, filed March 27, 2001.
FIELD OF THE INVENTION
This invention relates to the field of plant biology. More particularly, the present invention pertains to compositions and methods for phenotypically modifying a plant.
BACKGROUND OF THE INVENTION Transcription factors can modulate gene expression, either increasing or decreasing (inducing or repressing) the rate of transcription. This modulation results in differential levels of gene expression at various developmental stages, in different tissues and cell types, and in response to different exogenous (e.g., environmental) and endogenous stimuli throughout the life cycle of the organism. Because transcription factors are key controlling elements of biological pathways, altering the expression levels of one or more transcription factors can change entire biological pathways in an organism. For example, manipulation of the levels of selected transcription factors may result in increased expression of economically useful proteins or metabolic chemicals in plants or in improvement in other agriculturally relevant characteristics. Conversely, blocked or reduced expression of a transcription factor may reduce biosynthesis of unwanted compounds or remove an undesirable trait. Therefore, manipulating transcription factor levels in a plant offers tremendous potential in agricultural biotechnology for modifying a plant's traits. In order to maximize reproductive success, plants have evolved complex mechanisms to ensure that flowering occurs under favorable conditions. Analysis of late flowering mutants and ecotypes in Arabidopsis has revealed that such mechanisms are based upon several genetic pathways which may contain 80 or more loci. Together these loci co-ordinate flowering time with environmental variables (e.g. day-length, temperature, light quality, and nutrient availability) and with the developmental stage of the plant . We have discovered transcription factors that regulate flowering phenotypes, and in particular that regulate timing of the onset of reproductive development, the duration of the phase in which floral meristems are initiated, or the duration of time for which floral organs persist prior to their abscission, or the number of flowers generated on a plant. These transcription factors therefore are useful to manipulate flowering phenotypes of a plant.
SUMMARY OF THE INVENTION
In a first aspect, the invention relates to a recombinant polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence encoding a polypeptide comprising a sequence selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 and 26, or a complementary nucleotide sequence thereof; (b) a nucleotide sequence encoding a polypeptide comprising a conservatively substituted variant of a polypeptide of (a); (c) a nucleotide sequence comprising a sequence selected from those of SEQ ID NO: 1, 3, 5, 7, 9, 11 , 13, 15, 17, 19, 21, 23 and 25, or a complementary nucleotide sequence thereof; (d) a nucleotide sequence comprising silent substitutions in a nucleotide sequence of (c); (e) a nucleotide sequence which hybridizes under stringent conditions over substantially the entire length of a nucleotide sequence of one or more of: (a), (b), (c), or (d); (f) a nucleotide sequence comprising at least 15 consecutive nucleotides of a sequence of any of (a)-(e); (g) a nucleotide sequence comprising a subsequence or fragment of any of (a)-(f), which subsequence or fragment encodes a polypeptide having a biological activity that modifies a plant's flowering phenotype; (h) a nucleotide sequence having at least 40% sequence identity to a nucleotide sequence of any of (a)-(g); (i) a nucleotide sequence having at least 85% sequence identity to a nucleotide sequence of any of (a)-(g); (j) a nucleotide sequence which encodes a polypeptide having at least 40% sequence identity to a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26; (k) a nucleotide sequence which encodes a polypeptide having at least 85% identity sequence identity to a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26; (1) a nucleotide sequence which encodes a conserved domain of a polypeptide having at least 65% sequence identity to a conserved domain of a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26; (m) a nucleotide sequence which encodes a plant transcription factor comprising a fragment having 6 consecutive amino acids from a region of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 outside of the conserved domain; (n) a nucleotide sequence having at least 85% sequence identity to (m); (o) a nucleotide sequence which encodes a plant transcription factor comprising a fragment having 8 consecutive amino acids from a region of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 outside of the conserved domain; and (p) a nucleotide sequence having at least 85% sequence identity to (o). Homologous sequences, paralogs, orthologs and/or homologs as described herein or recited in the Tables are also specifically included in the polynucleotides and polypeptides of the invention. The recombinant polynucleotide may further comprise a constitutive, inducible, or tissue-specific promoter operably linked to the nucleotide sequence. The invention also relates to compositions comprising at least two of the above described polynucleotides. In a second aspect, the invention is an isolated or recombinant polypeptide comprising a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotide described above. These polynucleotides and polypeptides are useful for modifying the flowering phenotypes of a plant, and in particular for modifying timing of the onset of reproductive development, the duration of the phase in which floral meristems are initiated, the duration of time for which floral organs persist prior to their abscission, or the number of flowers generated on a plant.
In another aspect, the invention is a transgenic plant comprising one or more of the above described recombinant polynucleotides. In yet another aspect, the invention is a plant with altered expression levels of a polynucleotide described above or a plant with altered expression or activity levels of an above described polypeptide. In a further aspect, the invention relates to a cloning or expression vector comprising the isolated or recombinant polynucleotide described above or cells comprising the cloning or expression vector. In yet a further aspect, the invention relates to a composition produced by incubating a polynucleotide of the invention with a nuclease, a restriction enzyme, a polymerase, a polymerase and a primer, a cloning vector, or with a cell. Furthermore, the invention relates to a method for producing a plant having a modified flowering phenotype, such as flowering time or flowering period. The method comprises altering the expression of an isolated or recombinant polynucleotide of the invention or altering the expression or activity of a polypeptide of the invention in a plant to produce a modified plant, and selecting the modified plant for a modified flowering time or flowering period phenotype.
In another aspect, the invention relates to a method of identifying a factor that is modulated by or interacts with a polypeptide encoded by a polynucleotide of the invention. The method comprises expressing a polypeptide encoded by the polynucleotide in a plant and identifying at least one factor that is modulated by or interacts with the polypeptide. In one embodiment the method for identifying modulating or interacting factors is by detecting binding by the polypeptide to a promoter sequence, or by detecting interactions between an additional protein and the polypeptide in a yeast two hybrid system, or by detecting expression of a factor by hybridization to a microarray, subtractive hybridization or differential display.
In yet another aspect, the invention is a method of identifying a molecule that modulates activity or expression of a polynucleotide or polypeptide of interest. The method comprises placing the molecule in contact with a plant comprising the polynucleotide or polypeptide encoded by the polynucleotide of the invention and monitoring one or more of the expression level of the polynucleotide in the plant, the expression level of the polypeptide in the plant, and modulation of an activity of the polypeptide in the plant.
In yet another aspect, the invention relates to an integrated system, computer or computer readable medium comprising one or more character strings corresponding to a polynucleotide of the invention, or to a polypeptide encoded by the polynucleotide. The integrated system, computer or computer readable medium may comprise a link between one or more sequence strings to a modified plant flowering phenotype. In yet another aspect, the invention is a method for identifying a sequence similar or homologous to one or more polynucleotides of the invention, or one or more polypeptides encoded by the polynucleotides. The method comprises providing a sequence database and querying the sequence database with one or more target sequences corresponding to the one or more polynucleotides or to the one or more polypeptides to identify one or more sequence members of the database that display sequence similarity or homology to one or more of the target sequences. The method may further comprise linking the one or more polynucleotides of the invention, or encoded polypeptides, to a modified plant flowering phenotype.
BRIEF DESCRIPTION OF THE SEQUENCE LISTING
The Sequence Listing provides exemplary polynucleotide and polypeptide sequences of the invention. These sequences may be employed to modify the flowering time or the flowering period of a plant.
DETAILED DESCRIPTION
Each of the references, documents or information sources listed herein is specifically incorporated into this application and can be relied on to make or use the invention.
The present invention relates to polynucleotides and polypeptides, e.g. for modifying phenotypes of plants. In particular, the polynucleotides or polypeptides are useful for modifying traits associated with a plant's flowering time or flowering period when the expression levels of the polynucleotides or expression levels or activity levels of the polypeptides are altered compared with those found in a wild type plant. The flowering time of plants can be either decreased, increased or made inducible under specific conditions using the polynucleotides or polypeptides of this invention. These polynucleotides and polypeptides are also useful for modifying the duration of the phase in which floral meristems are initiated, the duration of time for which floral organs persist prior to their abscission, or the number of flowers generated on a plant. Additionally, the polynucleotides and polypeptides are useful for modifying traits associated with modified vernalization requirements or flowering time characteristics, such as changes in flowering time in response to day-length, in response to temperature, in response to light quality, nutrient availability, and development stage of the plant, the length of flowering time which delays senescence and the like.
The polynucleotides of the invention encode plant transcription factors. The plant transcription factors are derived, e.g., from Arabidopsis thaliana and can belong, e.g., to one or more of the following transcription factor families: the AP2
(APETALA2) domain transcription factor family (Riechmann and Meyerowitz (1998) Biol. Chem. 379:633-646); the MYB transcription factor family (Martin and Paz-Ares (1997) Trends Genet. 13:67-73); the MADS domain transcription factor family (Riechmann and Meyerowitz (1997) Biol. Chem. 378:1079-1101); the WRKY protein family (Ishiguro and Nakamura (1994) Mol. Gen. Genet. 244:563-571); the ankyrin- repeat protein family (Zhang et al. (1992) Plant Cell 4:1575-1588); the miscellaneous protein (MISC) family (Kim et al. (1997) Plant J. 11 : 1237-1251); the zinc finger protein (Z) family (Klug and Schwabe (1995) FASEB J. 9: 597-604); the homeobox (HB) protein family (Buerglin in Duboule (1994) Guidebook to the Homeobox Genes, Oxford University Press); the CAAT-element binding proteins (Forsburg and
Guarente (1989) Genes Dev. 3: 1166-1 178); the squamosa promoter binding proteins (SPB) (Klein et al. (1996) Mol. Gen. Genet. 1996 250:7-16); the NAM protein family; the IAA AUX proteins (Rouse et al. (1998) Science 279: 1371-1373); the HLH/MYC protein family (Littlewood et al. (1994) Prot. Profile 1 :639-709); the DNA-binding protein (DBP) family ( ucker et al. (1994) EMBO J. 13 :2994-3002); the bZIP family of transcription factors (Foster et al. (1994) FASEB J. 8: 192-200); the BPF-1 protein (Box P-binding factor) family (da Costa e Silva et al. (1993) Plant J. 4: 125-135); and the golden protein (GLD) family (Hall et al. (1998) Plant Cell 10:925-936). Exemplary transcription factors of the present invention are listed in the Sequence Listing.
In addition to methods for modifying a plant phenotype by employing one or more polynucleotides and polypeptides of the invention described herein, the polynucleotides and polypeptides of the invention have a variety of additional uses. These uses include their use in the recombinant production (i.e, expression) of proteins; as regulators of plant gene expression, as diagnostic probes for the presence of complementary or partially complementary nucleic acids (including for detection of natural coding nucleic acids), as substrates for further reactions, e.g., mutation reactions, PCR reactions, or the like, as substrates for cloning e.g., including digestion or ligation reactions, and for identifying exogenous or endogenous modulators of the transcription factors.
DEFINITIONS
A "polynucleotide" is a nucleic acid sequence comprising a plurality of polymerized nucleotide residues, e.g., at least about 15 consecutive polymerized nucleotide residues, optionally at least about 30 consecutive nucleotides, or at least about 50 consecutive nucleotides. In many instances, a polynucleotide comprises a nucleotide sequence encoding a polypeptide (or protein) or a domain or fragment thereof. Additionally, the polynucleotide may comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 5' or 3' untranslated regions, a reporter gene, a selectable marker, a purification tag, or the like. The polynucleotide can be single stranded or double stranded DNA or RNA. The polynucleotide optionally comprises modified bases or a modified backbone. The polynucleotide can be, e.g., genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like. The polynucleotide can comprise a sequence in either sense or antisense orientations.
A "recombinant polynucleotide" is a polynucleotide that is not in its native state, e.g., the polynucleotide comprises a nucleotide sequence not found in nature, or the polynucleotide is in a context other than that in which it is naturally found, e.g., separated from nucleotide sequences with which it typically is in proximity in nature, or adjacent (or contiguous with) nucleotide sequences with which it typically is not in proximity. For example, the sequence at issue can be cloned into a vector, or otherwise recombined with one or more additional nucleic acid.
An "isolated polynucleotide" is a polynucleotide whether naturally occurring or recombinant, that is present outside the cell in which it is typically found in nature, whether purified or not. Optionally, an isolated polynucleotide is subject to one or more enrichment or purification procedures, e.g., cell lysis, extraction, centrifugation, precipitation, or the like. A "recombinant polypeptide" is a polypeptide produced by translation of a recombinant polynucleotide. An "isolated polypeptide," whether a naturally occurring or a recombinant polypeptide, is more enriched in (or out of) a cell than the polypeptide in its natural state in a wild type cell, e.g., more than about 5% enriched, more than about 10% enriched, or more than about 20%, or more than about 50%, or more, enriched, i.e., alternatively denoted: 105%, 110%, 120%, 150% or more, enriched relative to wild type standardized at 100%. Such an enrichment is not the result of a natural response of a wild type plant. Alternatively, or additionally, the isolated polypeptide is separated from other cellular components with which it is typically associated, e.g., by any of the various protein purification methods herein. The term "transgenic plant" refers to a plant that contains genetic material, not found in a wild type plant of the same species, variety or cultivar. The genetic material may include a transgene, an insertional mutagenesis event (such as by transposon or T-DNA insertional mutagenesis), an activation tagging sequence, a mutated sequence, a homologous recombination event or a sequence modified by chimeraplasty. Typically, the foreign genetic material has been introduced into the plant by human manipulation.
A transgenic plant may contain an expression vector or cassette. The expression cassette typically comprises a polypeptide-encoding sequence operably linked (i.e., under regulatory control of) to appropriate inducible or constitutive regulatory sequences that allow for the expression of polypeptide. The expression cassette can be introduced into a plant by transformation or by breeding after transformation of a parent plant. A plant refers to a whole plant as well as to a plant part, such as seed, fruit, leaf, or root, plant tissue, plant cells or any other plant material, e.g., a plant explant, as well as to progeny thereof, and to in vitro systems that mimic biochemical or cellular components or processes in a cell.
The phrase "ectopically expression or altered expression" in reference to a polynucleotide indicates that the pattern of expression in, e.g., a transgenic plant or plant tissue, is different from the expression pattern in a wild type plant or a reference plant of the same species. For example, the polynucleotide or polypeptide is expressed in a cell or tissue type other than a cell or tissue type in which the sequence is expressed in the wild type plant, or by expression at a time other than at the time the sequence is expressed in the wild type plant, or by a response to different inducible agents, such as hormones or environmental signals, or at different expression levels (either higher or lower) compared with those found in a wild type plant. The term also refers to altered expression patterns that are produced by lowering the levels of expression to below the detection level or completely abolishing expression. The resulting expression pattern can be transient or stable, constitutive or inducible. In reference to a polypeptide, the term "ectopic expression or altered expression" may further relate to altered activity levels resulting from the interactions of the polypeptides with exogenous or endogenous modulators or from interactions with factors or as a result of the chemical modification of the polypeptides.
The term "fragment" or "domain," with respect to a polypeptide, refers to a subsequence of the polypeptide. In some cases, the fragment or domain is a subsequence of the polypeptide which performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide. For example, a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA binding domain that binds to a DNA promoter region, an activation domain or a domain for protein-protein interactions. Fragments can vary in size from as few as 5, 6 or 8 amino acids to the full length of the intact polypeptide, but are preferably at least about 30 amino acids in length and more preferably at least about 60 amino acids in length. In reference to a nucleotide sequence, "a fragment" refers to any subsequence of a polynucleotide, typically, of at least consecutive about 15 nucleotides, encoding 5, 6, 8, or 10 amino acids for example, preferably at least about 30 nucleotides, more preferably at least about 50, of any of the sequences provided herein. A fragment can consist of or comprise nucleotides encoding amino acids outside of a conserved domain known to exist in a particular transcription factor belonging to a transcription factor family, for example.
The term "trait" refers to a physiological, morphological, biochemical or physical characteristic of a plant or particular plant material or cell. In some instances, this characteristic is visible to the human eye, such as seed or plant size, or can be measured by available biochemical techniques, such as the protein, starch or oil content of seed or leaves or by the observation of the expression level of genes, e.g., by employing Northern analysis, RT-PCR, microarray gene expression assays or reporter gene expression systems, or by agricultural observations such as stress tolerance, yield or pathogen tolerance. "Trait modification" refers to a detectable difference in a characteristic in a plant ectopically expressing a polynucleotide or polypeptide of the present invention relative to a plant not doing so, such as a wild type plant. In some cases, the trait modification can be evaluated quantitatively. For example, the trait modification can entail at least about a 2% increase or decrease in an observed trait (difference), at least about a 5% difference, at least about a 10% difference, at least about a 20% difference, at least about a 30%, at least about a 50%, at least about a 70%, or at least about a 100%, or an even greater difference. It is known that there can be a natural variation in the modified trait. Therefore, the trait modification observed entails a change of the normal distribution of the trait in the plants compared with the distribution observed in wild type plant.
Trait modifications of particular interest include those to seed (such as embryo or endosperm), fruit, root, flower, leaf, stem, shoot, seedling or the like, including: enhanced tolerance to environmental conditions including freezing, chilling, heat, drought, water saturation, radiation and ozone; improved tolerance to microbial, fungal or viral diseases; improved tolerance to pest infestations, including nematodes, mollicutes, parasitic higher plants or the like; decreased herbicide sensitivity; improved tolerance of heavy metals or enhanced ability to take up heavy metals; improved growth under poor photoconditions (e.g., low light and/or short day length); or changes in expression levels of genes of interest. Other phenotypes that can be modified relate to the production of plant metabolites, such as variations in the production of taxol, tocopherol, tocotrienol, sterols, phytosterols, vitamins, wax monomers, anti-oxidants, amino acids, lignins, cellulose, tannins, prenyllipids (such as chlorophylls and carotenoids), glucosinolates, and terpenoids, enhanced or compositionally altered protein or oil production (especially in seeds), or modified sugar (insoluble or soluble) and/or starch composition. Physical plant characteristics that can be modified include cell development (such as the number of trichomes), fruit and seed size and number, yields of plant parts such as stems, leaves and roots, the stability of the seeds during storage, characteristics of the seed pod (e.g., susceptibility to shattering), root hair length and quantity, internode distances, or the quality of seed coat. Plant growth characteristics that can be modified include growth rate, germination rate of seeds, vigor of plants and seedlings, leaf and flower senescence, male sterility, apomixis, flowering time, flowering period, flower abscission, rate of nitrogen uptake, biomass or transpiration characteristics, as well as plant architecture characteristics such as apical dominance, branching patterns, number of organs, organ identity, organ shape or size.
POLYPEPTIDES AND POLYNUCLEOTIDES OF THE INVENTION The present invention provides, among other things, transcription factors (TFs), and transcription factor homologue polypeptides, and isolated or recombinant polynucleotides encoding the polypeptides. These polypeptides and polynucleotides may be employed to modify a plant's flowering phenotype.
Exemplary polynucleotides encoding the polypeptides of the invention were identified in the Arabidopsis thaliana GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. Polynucleotide sequences meeting such criteria were confirmed as transcription factors.
Additional polynucleotides of the invention were identified by screening Arabidopsis thaliana and/or other plant cDNA libraries with probes corresponding to known transcription factors under low stringency hybridization conditions. Additional sequences, including full length coding sequences were subsequently recovered by the rapid amplification of cDNA ends (RACE) procedure, using a commercially available kit according to the manufacturer's instructions. Where necessary, multiple rounds of RACE are performed to isolate 5' and 3' ends. The full length cDNA was then recovered by a routine end-to-end polymerase chain reaction (PCR) using primers specific to the isolated 5' and 3' ends. Exemplary sequences are provided in the Sequence Listing. The polynucleotides of the invention were ectopically expressed in overexpressor or knockout plants and changes in the flowering phenotype of the plants was observed. Therefore, the polynucleotides and polypeptides can be employed to improve the flowering phenotype of plants. Making polynucleotides
The polynucleotides of the invention include sequences that encode transcription factors and transcription factor homologue polypeptides and sequences complementary thereto, as well as unique fragments of coding sequence, or sequence complementary thereto. Such polynucleotides can be, e.g., DNA or RNA, mRNA, cRNA, synthetic RNA, genomic DNA, cDNA synthetic DNA, oligonucleotides, etc. The polynucleotides are either double-stranded or single-stranded, and include either, or both sense (i.e., coding) sequences and antisense (i.e., non-coding, complementary) sequences. The polynucleotides include the coding sequence of a transcription factor, or transcription factor homologue polypeptide, in isolation, in combination with additional coding sequences (e.g., a purification tag, a localization signal, as a fusion- protein, as a pre-protein, or the like), in combination with non-coding sequences (e.g., introns or inteins, regulatory elements such as promoters, enhancers, terminators, and the like), and/or in a vector or host environment in which the polynucleotide encoding a transcription factor or transcription factor homologue polypeptide is an endogenous or exogenous gene.
A variety of methods exist for producing the polynucleotides of the invention. Procedures for identifying and isolating DNA clones are well known to those of skill in the art, and are described in, e.g., Berger and Kimmel, Guide to Molecular Cloning Techniques. Methods in Enzymology volume 152 Academic Press, Inc., San Diego, CA ("Berger"); Sambrook et al., Molecular Cloning - A
Laboratory Manual (2nd Ed.), Vol. 1-3, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989 ("Sambrook") and Current Protocols in Molecular Biology. F.M. Ausubel et al., eds., Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (supplemented through 2000) ("Ausubel").
Alternatively, polynucleotides o the invention can be produced by a variety of in vitro amplification methods adapted to the present invention by appropriate selection of specific or degenerate primers. Examples of protocols sufficient to direct persons of skill through in vitro amplification methods, including the polymerase chain reaction (PCR) the ligase chain reaction (LCR), Qbeta-replicase amplification and other RNA polymerase mediated techniques (e.g., NASBA), e.g., for the production of the homologous nucleic acids of the invention are found in Berger, Sambrook, and Ausubel, as well as Mullis et al., (1987) PCR Protocols A Guide to Methods and Applications (Innis et al. eds) Academic Press Inc. San Diego, CA (1990) (Innis). Improved methods for cloning in vitro amplified nucleic acids are described in Wallace et al., U.S. Pat. No. 5,426,039. Improved methods for amplifying large nucleic acids by PCR are summarized in Cheng et al. (1994) Nature 369: 684-685 and the references cited therein, in which PCR amplicons of up to 40kb are generated. One of skill will appreciate that essentially any RNA can be converted into a double stranded DNA suitable for restriction digestion, PCR expansion and sequencing using reverse transcriptase and a polymerase. See, e.g., Ausubel, Sambrook and Berger, supra.
Alternatively, polynucleotides and oligonucleotides of the invention can be assembled from fragments produced by solid-phase synthesis methods. Typically, fragments of up to approximately 100 bases are individually synthesized and then enzymatically or chemically ligated to produce a desired sequence, e.g., a polynucletotide encoding all or part of a transcription factor. For example, chemical synthesis using the phosphoramidite method is described, e.g., by Beaucage et al. (1981) Tetrahedron Letters 22: 1859-69; and Matthes et al. (1984) EMBO J. 3:801-5. According to such methods, oligonucleotides are synthesized, purified, annealed to their complementary strand, ligated and then optionally cloned into suitable vectors. And if so desired, the polynucleotides and polypeptides of the invention can be custom ordered from any of a number of commercial suppliers.
HOMOLOGOUS SEQUENCES
Sequences homologous, i.e., that share significant sequence identity or similarity, to those provided in the Sequence Listing, derived from Arabidopsis thaliana or from other plants of choice are also an aspect of the invention.
Homologous sequences can be derived from any plant including monocots and dicots and in particular agriculturally important plant species including, but not limited to, crops such as soybean, wheat, corn, potato, cotton, rice, oilseed rape (including canola), sunflower, alfalfa, sugarcane and turf; or fruits and vegetables, such as banana, blackberry, blueberry, strawberry, and raspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant, grapes, honeydew, lettuce, mango, melon, onion, papaya, peas, peppers, pineapple, spinach, squash, sweet corn, tobacco, tomato, watermelon, rosaceous fruits (such as apple, peach, pear, cherry and plum) and vegetable brassicas (such as broccoli, cabbage, cauliflower, brussel sprouts and kohlrabi). Other crops, fruits and vegetables whose phenotype can be changed include barley, rye, millet, sorghum, currant, avocado, citrus fruits such as oranges, lemons, grapefruit and tangerines, artichoke, cherries, nuts such as the walnut and peanut, endive, leek, roots, such as arrowroot, beet, cassava, turnip, radish, yam, and sweet potato, and beans. The homologous sequences may also be derived from woody species, such pine, poplar and eucalyptus. Transcription factors that are homologous to the listed sequences will typically share at least about 35% amino acid sequence identity. More closely related transcription factors can share at least about 50%, about 60%, about 65%, about 70%, about 75% or about 80% or about 90% or about 95% or about 98% or more sequence identity with the listed sequences. Factors that are most closely related to the listed sequences share, e.g., at least about 85%, about 90% or about 95% or more sequence identity to the listed sequences. At the nucleotide level, the sequences will typically share at least about 40% nucleotide sequence identity, preferably at least about 50%, about 60%, about 70% or about 80% sequence identity, and more preferably about 85%, about 90%, about 95% or about 97% or more sequence identity to one or more of the listed sequences. The degeneracy of the genetic code enables major variations in the nucleotide sequence of a polynucleotide while maintaining the amino acid sequence of the encoded protein. Conserved domains within a transcription factor family may exhibit a higher degree of sequence homology, such as at least 65% sequence identity including conservative substitutions, and preferably at least 80% sequence identity. Exemplary conserved domains of the present invention include: for G2010 (SEQ ID NO: 7 and 8) amino acid residues 54 through 127, for G1037 (SEQ ID NO: 9 and 10) amino acid residues 11 through 134 or 200 through 248, for G1820 (SEQ ID NO: 5 and 6) amino acid residues 41-159, for G 1760 (SEQ ID NO: 3 and 4) amino acid residues 2 through 57, and for G590 (SEQ ID NO: 1 and 2) amino acid residues 193 through 253. Transcription factors of the invention can also contain 5, 6, 8, 10 or 12 consecutive amino acids from the sequences of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 where the consecutive amino acids are taken from a region outside of the conserved domain. Polynucleotides having at least 90%, or at least 85%, or at least 75%, or at least 60%, or at least 50%, or at least 40% sequence identity to those encoding the above transcription factors are also included in this invention. Orthologs and Paralogs
Several different methods are known by those of skill in the art for identifying and defining these functionally homologous sequences. Three general methods for defining paralogs and orthologs are described; a paralog or ortholog may be identified by only one or more of the methods described below. Orthologs and paralogs are evolutionarily related genes that have similar sequences and similar functions. Paralogs are related genes within a single species and are most likely a result of gene duplication, whereas orthologs are related genes in different species derived from a common ancestral molecule prior to speciation. Within a single plant species, gene duplication may cause two copies of a particular gene, giving rise to two or more genes with similar sequence and similar function known as paralogs. A paralog is therefore a similar gene with a similar function within the same species. Paralogs typically cluster together or in the same clade (a group of similar genes), as is shown when a gene family phylogeny is analyzed using programs such as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22:4673-4680; Higgins et al. (1996) Methods Enzymol. 266 383-402). Groups of similar genes can also be identified using by pair-wise BLAST analysis (Feng and Doolittle (1987) J. Mol. Evol. 25:351-360). For example, a clade of very similar MADS domain transcription factors from Arabidopsis all share a common function in flowering time (Ratcliffe et al. (2001) Plant Physiol. 126: 122-132), and a group of very similar AP2 domain transcription factors from Arabidopsis are involved in tolerance of plants to freezing (Gilmour et al. (1998) Plant J. 16:433-442). Analysis of groups of similar genes with similar function that fall within one clade can yield subsequences that are particular to the clade. These subsequences, known as consensus sequences, can not only be used to define the sequences within each clade, but define the functions of these genes, since genes within each clade typically share the same function. (See also, for example, Mount, D.W. (2001) Bioinformatics: Sequence and Genome Analysis Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York page 543.)
Speciation, the production of new species from a parental species, can also give rise to two or more genes with similar sequence and similar function. These genes, termed orthologs, often have an identical function within their host plants and are often interchangeable between species without losing function. Because plants have common ancestors, many genes in any plant species will have a corresponding orthologous gene in another plant species. Once a phylogenic tree for a gene family of one species has been constructed using a program such as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22:4673-4680; Higgins et al. (1996) Methods Enzymol. 266:383-402), potential orthologous sequences can placed into the phylogenetic tree and their relationship to genes from the species of interest can be determined. Once the ortholog pair has been identified, the function of the test ortholog can be determined by determining the function of the reference ortholog. Orthologs can also be identified by pair-wise BLAST analysis by aligning a set of reference sequences against a set of test sequences. Test sequences with the closest match to a particular reference sequence, as determined by the P- value of the BLAST analysis, can be taken and individually aligned against the reference set of sequences. The individual test sequence will either best match the particular reference sequence, in which case it is likely to be an ortholog, or not, in which case it may not be an ortholog.
A further way of identifying an ortholog is by identifying a consensus sequence within the candidate ortholog. Using pair-wise BLAST analysis, or programs such as CLUSTAL alignment program, sets of similar genes, or clades, can be identified. The particular subsequences which define commonalities within a particular clade can be derived from an alignment of those sequences. Orthologs would have the consensus sequence, or a sequence similar to the consensus sequence. Orthologs might also have a consensus sequence outside a conserved domain, which could be particular to that family of orthologous sequences.
Corresponding orthologs may bridge the monocot/dicot division of the plant kingdom and orthologous pairs of genes can be identified in rice and Arabidopsis, corn and Arabidopsis and Antirhinnum and com. For example Peng et al showed that a mutant of the Arabidopsis gene termed Gibberellin fnsensitive (GAI; mutant termed gai) encoded a transcription factor and which conferred a reduction in gibberellin responsiveness in the native plant (Peng et al. 1997 Genes and Development 11 :3194-3205). In addition, Peng et al. subsequently showed that the Arabidopsis GAI protein has 62 % amino acid residue identity with the wheat Rht- Dla protein and 62 % amino acid residue identity with the maize d8. Peng et al. showed that transgenic rice plants containing a mutant GAI allele give reduced response to gibberellin and are dwarfed, mimicking the dwarfed wheat variety from which the mutant Rht-Dla gene was isolated. Peng et al. taught that Arabidopsis GAI protein is an ortholog of the wheat Rht-Dla and maize d8 proteins. (Peng et al. 1999 Nature 400:256-261.)
In addition Fu et al. (2001 Plant Cell 13: 1791 -1802), Nandi et al. (2000 Curr. Biol. 10:215-218), Coupland (1995 Nature 377:482-483), and Weigel and Nilsson (1995 Nature 377:482-500) show that an Arabidopsis transcription factor expressed in an exogenous plant species elicits the same or very similar phenotypic response. Furthermore, Kater et al. (1998 Plant Cell 10: 171-182), Mandel et al. (1992 Cell 71-133-143), and Suzuki et al. (2001 Plant J. 28:409-418) showed that a transcription factor expressed in another plant species elicits the same or very similar phenotypic response of the endogenous sequence, as often predicted in earlier studies of Arabidopsis transcription factors in Arabidopsis.
Table 1 (appended to this application) lists a summary of orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search. The first column shows the polynucleotide sequence identifier (SEQ ID NO), the second column shows the transcription factor cDNA identifier (Gene ID), the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide sequence identified in a BLAST search (Test Sequence ID), the fourth column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability), the fifth column identifies the plant species of the Test Sequence (Test Sequence Species), and the sixth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation). Table 2 (appended to this application) lists orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search. The first column shows the polynucleotide sequence identifier (SEQ ID NO), the second column shows the transcription factor cDNA identifier (Gene ID), the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide (Test Sequence ID), the fourth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation), the fifth column shows the reading frame of the Test sequence encoding the orthologous or homologous sequence (Reading Frame), the sixth column shows the calculated score value of the aligned sequences (High Score), the seventh column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability), and the eighth column shows the number of regions in the Test Sequence that align with a sequence from the SEQ ID NO. (N).
Identifying Nucleic Acids by Hybridization Polynucleotides homologous to the sequences illustrated in the
Sequence Listing can be identified in a variety of ways known to one skilled in the art, e.g., by hybridization to each other under stringent or under highly stringent conditions. Single stranded polynucleotides hybridize when they associate based on a variety of well characterized physico-chemical forces, such as hydrogen bonding, solvent exclusion, base stacking and the like. The stringency of a hybridization reflects the degree of sequence identity of the nucleic acids involved, such that the higher the stringency, the more similar are the two polynucleotide strands. Stringency is influenced by a variety of factors, including temperature, salt concentration and composition, organic and non-organic additives, solvents, etc. present in both the hybridization and wash solutions and incubations (and number), as described in more detail in the references cited above. An example of stringent hybridization conditions for hybridization of complementary nucleic acids which have more than 100 complementary residues on a filter in a Southern or northern blot is about 5°C to 20°C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. The Tm is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a perfectly matched probe. Nucleic acid molecules that hybridize under stringent conditions will typically hybridize to a probe based on either the entire cDNA or selected portions, e.g., to a unique subsequence, of the cDNA under wash conditions of 0.2x SSC to 2.0 x SSC, 0.1% SDS at 50-65° C, for example 0.2 x SSC, 0.1% SDS at 65° C. For identification of less closely related homologues washes can be performed at a lower temperature, e.g., 50° C. In general, stringency is increased by raising the wash temperature and/or decreasing the concentration of SSC.
As another example, stringent conditions can be selected such that an oligonucleotide that is perfectly complementary to the coding oligonucleotide hybridizes to the coding oligonucleotide with at least about a 5-10x higher signal to noise ratio than the ratio for hybridization of the perfectly complementary oligonucleotide to a nucleic acid encoding a transcription factor known as of the filing date of the application. Conditions can be selected such that a higher signal to noise ratio is observed in the particular assay which is used, e.g., about 15x, 25x, 35x, 50x or more. Accordingly, the subject nucleic acid hybridizes to the unique coding oligonucleotide with at least a 2x higher signal to noise ratio as compared to hybridization of the coding oligonucleotide to a nucleic acid encoding known polypeptide. Again, higher signal to noise ratios can be selected, e.g., about 5x, lOx, 25x, 35x, 50x or more. The particular signal will depend on the label used in the relevant assay, e.g., a fluorescent label, a colorimetric label, a radio active label, or the like.
Alternatively, transcription factor homologue polypeptides can be obtained by screening an expression library using antibodies specific for one or more transcription factors. With the disclosed transcription factor and transcription factor homologue nucleic acid sequences, the encoded polypeptide(s) can be expressed and purified in a heterologous expression system (e.g., E. coli) and used to raise antibodies (monoclonal or polyclonal) specific for the polypeptide(s) in question. Antibodies can also be raised against synthetic peptides derived from transcription factor, or transcription factor homologue, amino acid sequences. Methods of raising antibodies are well known in the art and are described in Harlow and Lane (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, New York. Such antibodies can then be used to screen an expression library produced from the plant from which it is desired to clone additional transcription factor homologues, using the methods described above. The selected cDNAs can be confirmed by sequencing and enzymatic activity.
SEQUENCE VARIATIONS
It will readily be appreciated by those of skill in the art, that any of a variety of polynucleotide sequences are capable of encoding the transcription factors and transcription factor homologue polypeptides of the invention. Due to the degeneracy of the genetic code, many different polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing.
For example, Table 3 illustrates, e.g., that the codons AGC, AGT, TCA, TCC, TCG, and TCT all encode the same amino acid: serine. Accordingly, at each position in the sequence where there is a codon encoding serine, any of the above trinucleotide sequences can be used without altering the encoded polypeptide.
Table 3
Amino acids Codon
Alanine Ala A GCA GCC GCG GCU
Cysteine Cys C TGC TGT
Aspartic acid Asp D GAC GAT
Glutamic acid Glu E GAA GAG
Phenylalanine Phe F TTC TTT
Glycine Gly G GGA GGC GGG GGT
Histidine His H CAC CAT
Isoleucine He I ATA ATC ATT
Lysine Lys K AAA AAG
Leucine Leu L TTA TTG CTA CTC CTG CTT
Methionine Met M ATG
Asparagine Asn N AAC AAT
Proline Pro P CCA CCC CCG CCT
Glutamine Gin Q CAA CAG
Arginine Arg R AGA AGG CGA CGC CGG CGT
Serine Ser S AGC AGT TCA TCC TCG TCT
Threonine Thr T ACA ACC ACG ACT
Valine Val V GTA GTC GTG GTT
Tryptophan Trp w TGG
Tyrosine Tyr Y TAC TAT
Sequence alterations that do not change the amino acid sequence encoded by the polynucleotide are termed "silent" variations. With the exception of the codons ATG and TGG, encoding methionine and tryptophan, respectively, any of the possible codons for the same amino acid can be substituted by a variety of techniques, e.g., site-directed mutagenesis, available in the art. Accordingly, any and all such variations of a sequence selected from the above table are a feature of the invention.
In addition to silent variations, other conservative variations that alter one, or a few amino acids in the encoded polypeptide, can be made without altering the function of the polypeptide, these conservative variants are, likewise, a feature of the invention. For example, substitutions, deletions and insertions introduced into the sequences provided in the Sequence Listing are also envisioned by the invention. Such sequence modifications can be engineered into a sequence by site-directed mutagenesis (Wu (ed.) Meth. Enzymol. (1993) vol. 217, Academic Press) or the other methods noted below. Amino acid substitutions are typically of single residues; insertions usually will be on the order of about from 1 to 10 amino acid residues; and deletions will range about from 1 to 30 residues. In preferred embodiments, deletions or insertions are made in adjacent pairs, e.g., a deletion of two residues or insertion of two residues. Substitutions, deletions, insertions or any combination thereof can be combined to arrive at a sequence. The mutations that are made in the polynucleotide encoding the transcription factor should not place the sequence out of reading frame and should not create complementary regions that could produce secondary mRNA structure. Preferably, the polypeptide encoded by the DNA performs the desired function.
Conservative substitutions are those in which at least one residue in the amino acid sequence has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the Table 4 when it is desired to maintain the activity of the protein. Table 4 shows amino acids which can be substituted for an amino acid in a protein and which are typically regarded as conservative substitutions.
Table 4
Residue Conservative Substitutions
Ala Ser Arg Lys Asn Gin; His Asp Glu Gin Asn Cys Ser Glu Asp Gly Pro His Asn; Gin He Leu, Val Leu He; Val Lys Arg; Gin Met Leu; He Phe Met; Leu; Tyr Ser Thr; Gly Thr SerNal Trp Tyr Tyr Trp; Phe Val He; Leu
Substitutions that are less conservative than those in Table 4 can be selected by picking residues that differ more significantly in their effect on maintaining (a) the structure of the polypeptide backbone in the area of the substitution, for example, as a sheet or helical conformation, (b) the charge or hydrophobicity of the molecule at the target site, or (c) the bulk of the side chain. The substitutions which in general are expected to produce the greatest changes in protein properties will be those in which (a) a hydrophilic residue, e.g., seryl or threonyl, is substituted for (or by) a hydrophobic residue, e.g., leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain, e.g., lysyl, arginyl, or histidyl, is substituted for (or by) an electronegative residue, e.g., glutamyl or aspartyl; or (d) a residue having a bulky side chain, e.g., phenylalanine, is substituted for (or by) one not having a side chain, e.g., glycine.
FURTHER MODIFYING SEQUENCES OF THE INVENTION— MUTATION/ FORCED EVOLUTION
In addition to generating silent or conservative substitutions as noted, above, the present invention optionally includes methods of modifying the sequences of the Sequence Listing. In the methods, nucleic acid or protein modification methods are used to alter the given sequences to produce new sequences and/or to chemically or enzymatically modify given sequences to change the properties of the nucleic acids or proteins.
Thus, in one embodiment, given nucleic acid sequences are modified, e.g., according to standard mutagenesis or artificial evolution methods to produce modified sequences. For example, Ausubel, supra, provides additional details on mutagenesis methods. Artificial forced evolution methods are described, e.g., by Stemmer (1994) Nature 370:389-391, and Stemmer (1994) Proc. Natl. Acad. Sci. USA 91 : 10747- 10751. Many other mutation and evolution methods are also available and expected to be within the skill of the practitioner. Similarly, chemical or enzymatic alteration of expressed nucleic acids and polypeptides can be performed by standard methods. For example, sequence can be modified by addition of lipids, sugars, peptides, organic or inorganic compounds, by the inclusion of modified nucleotides or amino acids, or the like. For example, protein modification techniques are illustrated in Ausubel, supra. Further details on chemical and enzymatic modifications can be found herein. These modification methods can be used to modify any given sequence, or to modify any sequence produced by the various mutation and artificial evolution modification methods noted herein.
Accordingly, the invention provides for modification of any given nucleic acid by mutation, evolution, chemical or enzymatic modification, or other available methods, as well as for the products produced by practicing such methods, e.g., using the sequences herein as a starting substrate for the various modification approaches.
For example, optimized coding sequence containing codons preferred by a particular prokaryotic or eukaryotic host can be used e.g., to increase the rate of translation or to produce recombinant RNA transcripts having desirable properties, such as a longer half-life, as compared with transcripts produced using a on- optimized sequence. Translation stop codons can also be modified to reflect host preference. For example, preferred stop codons for S. cerevisiae and mammals are TAA and TGA, respectively. The preferred stop codon for monocotyledonous plants is TGA, whereas insects and E. coli prefer to use TAA as the stop codon.
The polynucleotide sequences of the present invention can also be engineered in order to alter a coding sequence for a variety of reasons, including but not limited to, alterations which modify the sequence to facilitate cloning, processing and/or expression of the gene product. For example, alterations are optionally introduced using techniques which are well known in the art, e.g., site-directed mutagenesis, to insert new restriction sites, to alter glycosylation patterns, to change codon preference, to introduce splice sites, etc.
Furthermore, a fragment or domain derived from any of the polypeptides of the invention can be combined with domains derived from other transcription factors or synthetic domains to modify the biological activity of a transcription factor. For instance, a DNA binding domain derived from a transcription factor of the invention can be combined with the activation domain of another transcription factor or with a synthetic activation domain. A transcription activation domain assists in initiating transcription from a DNA binding site. Examples include the transcription activation region of VP16 or GAL4 (Moore et al. (1998) Proc. Natl. Acad. Sci. USA 95: 376-381; and Aovama et al. (1995) Plant Cell 7: 1773-1785), peptides derived from bacterial sequences (Ma and Ptashne (1987) Cell 51; 1 13-119) and synthetic peptides (Giniger and Ptashne, (1987) Nature 330:670- 672).
EXPRESSION AND MODIFICATION OF POLYPEPTIDES
Typically, polynucleotide sequences of the invention are incoφorated into recombinant DNA (or RNA) molecules that direct expression of polypeptides of the invention in appropriate host cells, transgenic plants, in vitro translation systems, or the like. Due to the inherent degeneracy of the genetic code, nucleic acid sequences which encode substantially the same or a functionally equivalent amino acid sequence can be substituted for any listed sequence to provide for cloning and expressing the relevant homologue.
Vectors, Promoters and Expression Systems
The present invention includes recombinant constructs comprising one or more of the nucleic acid sequences herein. The constructs typically comprise a vector, such as a plasmid, a cosmid, a phage, a virus (e.g., a plant virus), a bacterial artificial chromosome (BAC), a yeast artificial chromosome (YAC), or the like, into which a nucleic acid sequence of the invention has been inserted, in a forward or reverse orientation. In a preferred aspect of this embodiment, the construct further comprises regulatory sequences, including, for example, a promoter, operably linked to the sequence. Large numbers of suitable vectors and promoters are known to those of skill in the art, and are commercially available.
General texts which describe molecular biological techniques useful herein, including the use and production of vectors, promoters and many other relevant topics, include Berger, Sambrook and Ausubel, supra. Any of the identified sequences can be incoφorated into a cassette or vector, e.g., for expression in plants. A number of expression vectors suitable for stable transformation of plant cells or for the establishment of transgenic plants have been described including those described in Weissbach and Weissbach, (1989J Methods for Plant Molecular Biology, Academic Press, and Gelvin et al., (1990) Plant Molecular Biology Manual, Kluwer Academic Publishers. Specific examples include those derived from a Ti plasmid of Agrobacterium tumefaciens, as well as those disclosed by Herrera-Estrella et al. (1983) Nature 303: 209, Bevan (1984) Nucl Acid Res. 12: 8711-8721, Klee (1985) Bio/Technology 3: 637-642, for dicotyledonous plants. Alternatively, non-Ti vectors can be used to transfer the DNA into monocotyledonous plants and cells by using free DNA delivery techniques. Such methods can involve, for example, the use of liposomes, electroporation, microprojectile bombardment, silicon carbide whiskers, and viruses. By using these methods transgenic plants such as wheat, rice (Christou (1991) Bio/Technology 9: 957-962) and com (Gordon-Kamm (1990) Plant Cell 2: 603-618) can be produced. An immature embryo can also be a good target tissue for monocots for direct DNA delivery techniques by using the particle gun (Weeks et al. (1993) Plant Physiol 102: 1077-1084; Vasil (1993) Bio/Technology 10: 667-674; Wan and Lemeaux (1994) Plant Physiol 104: 37-48, and for Agrobacterium-mediated DNA transfer (Ishida et al. (1996) Nature Biotech 14: 745-750).
Typically, plant transformation vectors include one or more cloned plant coding sequence (genomic or cDNA) under the transcriptional control of 5' and 3' regulatory sequences and a dominant selectable marker. Such plant transformation vectors typically also contain a promoter (e.g., a regulatory region controlling inducible or constitutive, environmentally-or developmentally-regulated, or cell- or tissue-specific expression), a transcription initiation start site, an RNA processing signal (such as intron splice sites), a transcription termination site, and/or a polyadenylation signal.
Examples of constitutive plant promoters which can be useful for expressing the TF sequence include: the cauliflower mosaic vims (CaMV) 35S promoter, which confers constitutive, high-level expression in most plant tissues (see, e.g., Odel et al. (1985) Nature 313:810); the nopaline synthase promoter (An et al.
(1988) Plant Physiol 88:547); and the octopine synthase promoter (Fromm et al.
(1989) Plant Cell 1: 977).
A variety of plant gene promoters that regulate gene expression in response to environmental, hormonal, chemical, developmental signals, and in a tissue-specific or preferential manner can be used for expression of a TF sequence in plants. Choice of a promoter is based largely on the phenotype of interest and is determined by such factors as tissue (e.g., seed, fruit, root, pollen, vascular tissue, flower, caφel, etc.), inducibility (e.g., in response to wounding, heat, cold, drought, light, pathogens, etc.), timing, developmental stage, and the like. Numerous known promoters have been characterized and can favorable be employed to promote expression of a polynucleotide of the invention in a transgenic plant or cell of interest. For example, tissue specific promoters include: seed-specific promoters (such as the napin, phaseolin or DC3 promoter described in US Pat. No. 5,773,697), fruit-specific promoters that are active during fruit ripening (such as the dru 1 promoter (US Pat. No. 5,783,393), or the 2A1 1 promoter (US Pat. No. 4,943,674) and the tomato polygalacturonase promoter (Bird et al. (1988) Plant Mol Biol 1 1 :651), root-specific promoters, such as those disclosed in US Patent Nos. 5,618,988, 5,837,848 and 5,905,186, pollen-active promoters such as PTA29, PTA26 and PTA13 (US Pat. No. 5,792,929), promoters active in vascular tissue (Ringli and Keller (1998) Plant Mol Biol 37:977-988), flower-specific (Kaiser et al, (1995) Plant Mol Biol 28:231-243). pollen (Baerson et al. (1994) Plant Mol Biol 26: 1947-1959), caφels (Ohl et al. (1990) Plant Cell 2:837-848), pollen and ovules (Baerson et al. (1993) Plant Mol Biol 22:255-267), auxin-inducible promoters (such as that described in van der Kop et al. (1999) Plant Mol Biol 39:979-990 or Baumann et al. (1999) Plant Cell 11 :323-334), cytokinin-inducible promoter (Guevara-Garcia (1998) Plant Mol Biol 38:743-753), promoters responsive to gibberellin (Shi et al. (1998) Plant Mol Biol 38:1053-1060, Willmott et al. (1998) 38:817-825) and the like. Additional promoters are those that elicit expression in response to heat (Ainley et al. (1993) Plant Mol Biol 22: 13-23), light (e.g., the pea rbcS-3A promoter, Kuhlemeier et al. (1989) Plant Cell 1:471, and the maize rbcS promoter, Schaffher and Sheen (1991) Plant Cell 3: 997); wounding (e.g., wunl, Siebertz et al. (1989) Plant Cell 1 : 961); pathogens (such as the PR-1 promoter described in Buchel et al. (1999) Plant Mol. Biol. 40:387-396, and the PDF 1.2 promoter described in Manners et al. (1998) Plant Mol. Biol. 38: 1071-80), and chemicals such as methyl jasmonate or salicylic acid (Gatz et al. (1997) Annu. Rev. Plant Physiol. Plant Mol Biol 48: 89-108). In addition, the timing of the expression can be controlled by using promoters such as those acting at senescence (Gan and
Amasino (1995) Science 270: 1986-1988); or late seed development (Odell et al. (1994) Plant Physiol 106:447-458).
Plant expression vectors can also include RNA processing signals that can be positioned within, upstream or downstream of the coding sequence. In addition, the expression vectors can include additional regulatory sequences from the 3 '-untranslated region of plant genes, e.g., a 3' terminator region to increase mRNA stability of the mRNA, such as the PI-II terminator region of potato or the octopine or nopaline synthase 3' terminator regions.
Additional Expression Elements Specific initiation signals can aid in efficient translation of coding sequences. These signals can include, e.g., the ATG initiation codon and adjacent sequences. In cases where a coding sequence, its initiation codon and upstream sequences are inserted into the appropriate expression vector, no additional translational control signals may be needed. However, in cases where only coding sequence (e.g., a mature protein coding sequence), or a portion thereof, is inserted, exogenous transcriptional control signals including the ATG initiation codon can be separately provided. The initiation codon is provided in the correct reading frame to facilitate transcription. Exogenous transcriptional elements and initiation codons can be of various origins, both natural and synthetic. The efficiency of expression can be enhanced by the inclusion of enhancers appropriate to the cell system in use. Expression Hosts
The present invention also relates to host cells which are transduced with vectors of the invention, and the production of polypeptides of the invention (including fragments thereof) by recombinant techniques. Host cells are genetically engineered (i.e, nucleic acids are introduced, e.g., transduced, transformed or transfected) with the vectors of this invention, which may be, for example, a cloning vector or an expression vector comprising the relevant nucleic acids herein. The vector is optionally a plasmid, a viral particle, a phage, a naked nucleic acids, etc. The engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants, or amplifying the relevant gene. The culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to those skilled in the art and in the references cited herein, including, Sambrook and Ausubel. The host cell can be a eukaryotic cell, such as a yeast cell, or a plant cell, or the host cell can be a prokaryotic cell, such as a bacterial cell. Plant protoplasts are also suitable for some applications. For example, the DNA fragments are introduced into plant tissues, cultured plant cells or plant protoplasts by standard methods including electroporation (Fromm et al., (1985) Proc. Natl. Acad. Sci. USA 82, 5824, infection by viral vectors such as cauliflower mosaic vims (CaMV) (Hohn et al., (1982) Molecular Biology of Plant Tumors, (Academic Press, New York) pp. 549-560; US 4,407,956), high velocity ballistic penetration by small particles with the nucleic acid either within the matrix of small beads or particles, or on the surface (Klein et al., (1987) Nature 327, 70-73), use of pollen as vector (WO 85/01856), or use of Agrobacterium tumefaciens or A. rhizogenes carrying a T-DNA plasmid in which DNA fragments are cloned. The T-DNA plasmid is transmitted to plant cells upon infection by Agrobacterium tumefaciens, and a portion is stably integrated into the plant genome (Horsch et al. (1984) Science 233:496-498: Fraley et al. (1983) Proc. Natl. Acad. Sci. USA 80, 4803). The cell can include a nucleic acid of the invention which encodes a polypeptide, wherein the cells expresses a polypeptide of the invention. The cell can also include vector sequences, or the like. Furthermore, cells and transgenic plants which include any polypeptide or nucleic acid above or throughout this specification, e.g., produced by transduction of a vector of the invention, are an additional feature of the invention.
For long-term, high-yield production of recombinant proteins, stable expression can be used. Host cells transformed with a nucleotide sequence encoding a polypeptide of the invention are optionally cultured under conditions suitable for the expression and recovery of the encoded protein from cell culture. The protein or fragment thereof produced by a recombinant cell may be secreted, membrane-bound, or contained intracellularly, depending on the sequence and/or the vector used. As will be understood by those of skill in the art, expression vectors containing polynucleotides encoding mature proteins of the invention can be designed with signal sequences which direct secretion of the mature polypeptides through a prokaryotic or eukaryotic cell membrane.
IDENTIFICATION OF ADDITIONAL FACTORS
A transcription factor provided by the present invention can also be used to identify additional endogenous or exogenous molecules that can affect a phentoype or trait of interest. On the one hand, such molecules include organic (small or large molecules) and/or inorganic compounds that affect expression of (i.e., regulate) a particular transcription factor. Alternatively, such molecules include endogenous molecules that are acted upon either at a transcriptional level by a transcription factor of the invention to modify a phenotype as desired. For example, the transcription factors can be employed to identify one or more downstream gene with which is subject to a regulatory effect of the transcription factor. In one approach, a transcription factor or transcription factor homologue of the invention is expressed in a host cell, e.g, a transgenic plant cell, tissue or explant, and expression products, either RNA or protein, of likely or random targets are monitored, e.g., by hybridization to a microarray of nucleic acid probes corresponding to genes expressed in a tissue or cell type of interest, by two-dimensional gel electrophoresis of protein products, or by any other method known in the art for assessing expression of gene products at the level of RNA or protein. Alternatively, a transcription factor of the invention can be used to identify promoter sequences (i.e., binding sites) involved in the regulation of a downstream target. After identifying a promoter sequence, interactions between the transcription factor and the promoter sequence can be modified by changing specific nucleotides in the promoter sequence or specific amino acids in the transcription factor that interact with the promoter sequence to alter a plant trait. Typically, transcription factor DNA binding sites are identified by gel shift assays. After identifying the promoter regions, the promoter region sequences can be employed in double-stranded DNA arrays to identify molecules that affect the interactions of the transcription factors with their promoters (Bulyk et al. (1999) Nature Biotechnology 17:573-577). The identified transcription factors are also useful to identify proteins that modify the activity of the transcription factor. Such modification can occur by covalent modification, such as by phosphorylation, or by protein-protein (homo or- heteropolymer) interactions. Any method suitable for detecting protein-protein interactions can be employed. Among the methods that can be employed are co- immunoprecipitation, cross-linking and co-purification through gradients or chromatographic columns, and the two-hybrid yeast system.
The two-hybrid system detects protein interactions in vivo and is described in Chien, et al., (1991), Proc. Natl. Acad. Sci. USA 88, 9578-9582 and is commercially available from Clontech (Palo Alto, Calif). In such a system, plasmids are constmcted that encode two hybrid proteins: one consists of the DNA-binding domain of a transcription activator protein fused to the TF polypeptide and the other consists of the transcription activator protein's activation domain fused to an unknown protein that is encoded by a cDNA that has been recombined into the plasmid as part of a cDNA library. The DNA-binding domain fusion plasmid and the cDNA library are transfomied into a strain of the yeast Saccharomyces cerevisiae that contains a reporter gene (e.g., lacZ) whose regulatory region contains the transcription activator's binding site. Either hybrid protein alone cannot activate transcription of the reporter gene. Interaction of the two hybrid proteins reconstitutes the functional activator protein and results in expression of the reporter gene, which is detected by an assay for the reporter gene product. Then, the library plasmids responsible for reporter gene expression are isolated and sequenced to identify the proteins encoded by the library plasmids. After identifying proteins that interact with the transcription factors, assays for compounds that interfere with the TF protein-protein interactions can be preformed.
IDENTIFICATION OF MODULATORS
In addition to the intracellular molecules described above, extracellular molecules that alter activity or expression of a transcription factor, either directly or indirectly, can be identified. For example, the methods can entail first placing a candidate molecule in contact with a plant or plant cell. The molecule can be introduced by topical administration, such as spraying or soaking of a plant, and then the molecule's effect on the expression or activity of the TF polypeptide or the expression of the polynucleotide monitored. Changes in the expression of the TF polypeptide can be monitored by use of polyclonal or monoclonal antibodies, gel electrophoresis or the like. Changes in the expression of the corresponding polynucleotide sequence can be detected by use of microarrays, Northerns, quantitative PCR, or any other technique for monitoring changes in mRNA expression. These techniques are exemplified in Ausubel et al. (eds) Current
Protocols in Molecular Biology, John Wiley & Sons (1998). Such changes in the expression levels can be correlated with modified plant traits and thus identified molecules can be useful for soaking or spraying on fruit, vegetable and grain crops to modify traits in plants. Essentially any available composition can be tested for modulatory activity of expression or activity of any nucleic acid or polypeptide herein. Thus, available libraries of compounds such as chemicals, polypeptides, nucleic acids and the like can be tested for modulatory activity. Often, potential modulator compounds can be dissolved in aqueous or organic (e.g., DMSO-based) solutions for easy delivery to the cell or plant of interest in which the activity of the modulator is to be tested. Optionally, the assays are designed to screen large modulator composition libraries by automating the assay steps and providing compounds from any convenient source to assays, which are typically mn in parallel (e.g., in microtiter formats on microtiter plates in robotic assays). In one embodiment, high throughput screening methods involve providing a combinatorial library containing a large number of potential compounds (potential modulator compounds). Such "combinatorial chemical libraries" are then screened in one or more assays, as described herein, to identify those library members (particular chemical species or subclasses) that display a desired characteristic activity. The compounds thus identified can serve as target compounds. A combinatorial chemical library can be, e.g., a collection of diverse chemical compounds generated by chemical synthesis or biological synthesis. For example, a combinatorial chemical library such as a polypeptide library is formed by combining a set of chemical building blocks (e.g., in one example, amino acids) in every possible way for a given compound length (i.e., the number of amino acids in a polypeptide compound of a set length). Exemplary libraries include peptide libraries, nucleic acid libraries, antibody libraries (see, e.g., Vaughn et al. (1996) Nature Biotechnology, 14(3):309-314 and PCT/US96/10287), carbohydrate libraries (see, e.g., Liang et al. Science (1996) 274: 1520-1522 and U.S. Patent 5,593,853), peptide nucleic acid libraries (see, e.g., U.S. Patent 5,539,083), and small organic molecule libraries (see, e.g., benzodiazepines, Bau C&EN Jan 18, page 33 (1993); isoprenoids, U.S. Patent 5,569,588; thiazolidinones and metathiazanones, U.S. Patent 5,549,974; pyrrolidines, U.S. Patents 5,525,735 and 5,519,134; moφholino compounds, U.S. Patent 5,506,337) and the like.
Preparation and screening of combinatorial or other libraries is well known to those of skill in the art. Such combinatorial chemical libraries include, but are not limited to, peptide libraries (see, e.g., U.S. Patent 5,010,175, Furka, Int. J. Pept. Prot. Res. 37:487-493 (1991) and Houghton et al. Nature 354:84-88 (1991)). Other chemistries for generating chemical diversity libraries can also be used. In addition, as noted, compound screening equipment for high- throughput screening is generally available, e.g., using any of a number of well known robotic systems that have also been developed for solution phase chemistries useful in assay systems. These systems include automated workstations including an automated synthesis apparatus and robotic systems utilizing robotic arms. Any of the above devices are suitable for use with the present invention, e.g., for high-throughput screening of potential modulators. The nature and implementation of modifications to these devices (if any) so that they can operate as discussed herein will be apparent to persons skilled in the relevant art. Indeed, entire high throughput screening systems are commercially available. These systems typically automate entire procedures including all sample and reagent pipetting, liquid dispensing, timed incubations, and final readings of the microplate in detector(s) appropriate for the assay. These configurable systems provide high throughput and rapid start up as well as a high degree of flexibility and customization. Similarly, microfluidic implementations of screening are also commercially available.
The manufacturers of such systems provide detailed protocols the various high throughput. Thus, for example, Zymark Coφ. provides technical bulletins describing screening systems for detecting the modulation of gene transcription, ligand binding, and the like. The integrated systems herein, in addition to providing for sequence alignment and, optionally, synthesis of relevant nucleic acids, can include such screening apparatus to identify modulators that have an effect on one or more polynucleotides or polypeptides according to the present invention. In some assays it is desirable to have positive controls to ensure that the components of the assays are working properly. At least two types of positive controls are appropriate. That is, known transcriptional activators or inhibitors can be incubated with cells/plants/ etc. in one sample of the assay, and the resulting increase/decrease in transcription can be detected by measuring the resulting increase in RNA/ protein expression, etc., according to the methods herein. It will be appreciated that modulators can also be combined with transcriptional activators or inhibitors to find modulators which inhibit transcriptional activation or transcriptional repression. Either expression of the nucleic acids and proteins herein or any additional nucleic acids or proteins activated by the nucleic acids or proteins herein, or both, can be monitored.
In an embodiment, the invention provides a method for identifying compositions that modulate the activity or expression of a polynucleotide or polypeptide of the invention. For example, a test compound, whether a small or large molecule, is placed in contact with a cell, plant (or plant tissue or explant), or composition comprising the polynucleotide or polypeptide of interest and a resulting effect on the cell, plant, (or tissue or explant) or composition is evaluated by monitoring, either directly or indirectly, one or more of: expression level of the polynucleotide or polypeptide, activity (or modulation of the activity) of the polynucleotide or polypeptide. In some cases, an alteration in a plant phenotype can be detected following contact of a plant (or plant cell, or tissue or explant) with the putative modulator, e.g., by modulation of expression or activity of a polynucleotide or polypeptide of the invention.
SUBSEQUENCES
Also contemplated are uses of polynucleotides, also referred to herein as oligonucleotides, typically having at least 12 bases, preferably at least 15, more preferably at least 20, 30, or 50 bases, which hybridize under at least highly stringent (or ultra-high stringent or ultra-ultra- high stringent conditions) conditions to a polynucleotide sequence described above. The polynucleotides may be used as probes, primers, sense and antisense agents, and the like, according to methods as noted supra. Subsequences of the polynucleotides of the invention, including polynucleotide fragments and oligonucleotides are useful as nucleic acid probes and primers. An oligonucleotide suitable for use as a probe or primer is at least about 15 nucleotides in length, more often at least about 18 nucleotides, often at least about 21 nucleotides, frequently at least about 30 nucleotides, or about 40 nucleotides, or more in length. A nucleic acid probe is useful in hybridization protocols, e.g., to identify additional polypeptide homologues of the invention, including protocols for microarray experiments. Primers can be annealed to a complementary target DNA strand by nucleic acid hybridization to form a hybrid between the primer and the target DNA strand, and then extended along the target DNA strand by a DNA polymerase enzyme. Primer pairs can be used for amplification of a nucleic acid sequence, e.g., by the polymerase chain reaction (PCR) or other nucleic-acid amplification methods. See Sambrook and Ausubel, supra.
In addition, the invention includes an isolated or recombinant polypeptide including a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotides of the invention. For example, such polypeptides, or domains or fragments thereof, can be used as immunogens, e.g., to produce antibodies specific for the polypeptide sequence, or as probes for detecting a sequence of interest. A subsequence can range in size from about 15 amino acids in length up to and including the full length of the polypeptide.
PRODUCTION OF TRANSGENIC PLANTS
Modification of Traits The polynucleotides of the invention are favorably employed to produce transgenic plants with various traits, or characteristics, that have been modified in a desirable manner, e.g., to improve the pathogen resistance of a plant. For example, alteration of expression levels or patterns (e.g., spatial or temporal expression patterns) of one or more of the transcription factors (or transcription factor homologues) of the invention, as compared with the levels of the same protein found in a wild type plant, can be used to modify a plant's traits. An illustrative example of trait modification, improved flowering phenotype, by altering expression levels of a particular transcription factor is described further in the Examples and the Sequence Listing. Antisense and Cosuppression Approaches
In addition to expression of the nucleic acids of the invention as gene replacement or plant phenotype modification nucleic acids, the nucleic acids are also useful for sense and anti-sense suppression of expression, e.g., to down-regulate expression of a nucleic acid of the invention, e.g., as a further mechanism for modulating plant phenotype. That is, the nucleic acids of the invention, or subsequences or anti-sense sequences thereof, can be used to block expression of naturally occurring homologous nucleic acids. A variety of sense and anti-sense technologies are known in the art, e.g., as set forth in Lichtenstein and Nellen (1997) Antisense Technology: A Practical Approach IRL Press at Oxford University, Oxford, England. In general, sense or anti-sense sequences are introduced into a cell, where they are optionally amplified, e.g., by transcription. Such sequences include both simple oligonucleotide sequences and catalytic sequences such as ribozymes.
For example, a reduction or elimination of expression (i.e., a "knockout") of a transcription factor or transcription factor homologue polypeptide in a transgenic plant, e.g., to modify a plant trait, can be obtained by introducing an antisense construct corresponding to the polypeptide of interest as a cDNA. For antisense suppression, the transcription factor or homologue cDNA is arranged in reverse orientation (with respect to the coding sequence) relative to the promoter sequence in the expression vector. The introduced sequence need not be the full length cDNA or gene, and need not be identical to the cDNA or gene found in the plant type to be transformed. Typically, the antisense sequence need only be capable of hybridizing to the target gene or RNA of interest. Thus, where the introduced sequence is of shorter length, a higher degree of homology to the endogenous transcription factor sequence will be needed for effective antisense suppression. While antisense sequences of various lengths can be utilized, preferably, the introduced antisense sequence in the vector will be at least 30 nucleotides in length, and improved antisense suppression will typically be observed as the length of the antisense sequence increases. Preferably, the length of the antisense sequence in the vector will be greater than 100 nucleotides. Transcription of an antisense construct as described results in the production of RNA molecules that are the reverse complement of mRNA molecules transcribed from the endogenous transcription factor gene in the plant cell.
Suppression of endogenous transcription factor gene expression can also be achieved using a ribozyme. Ribozymes are RNA molecules that possess highly specific endoribonuclease activity. The production and use of ribozymes are disclosed in U.S. Patent No. 4,987,071 and U.S. Patent No. 5,543,508. Synthetic ribozyme sequences including antisense RNAs can be used to confer RNA cleaving activity on the antisense RNA, such that endogenous mRNA molecules that hybridize to the antisense RNA are cleaved, which in rum leads to an enhanced antisense inhibition of endogenous gene expression. Vectors in which RNA encoded by a transcription factor or transcription factor homologue cDNA is over-expressed can also be used to obtain co- suppression of a corresponding endogenous gene, e.g., in the manner described in U.S. Patent No. 5,231,020 to Jorgensen. Such co-suppression (also termed sense suppression) does not require that the entire transcription factor cDNA be introduced into the plant cells, nor does it require that the introduced sequence be exactly identical to the endogenous transcription factor gene of interest. However, as with antisense suppression, the suppressive efficiency will be enhanced as specificity of hybridization is increased, e.g., as the introduced sequence is lengthened, and/or as the sequence similarity between the introduced sequence and the endogenous transcription factor gene is increased.
Vectors expressing an untranslatable form of the transcription factor mRNA, e.g., sequences comprising one or more stop codon, or nonsense mutation) can also be used to suppress expression of an endogenous transcription factor, thereby reducing or eliminating it's activity and modifying one or more traits. Methods for producing such constructs are described in U.S. Patent No. 5,583,021. Preferably, such constmcts are made by introducing a premature stop codon into the transcription factor gene. Alternatively, a plant trait can be modified by gene silencing using double-strand RNA (Shaφ (1999) Genes and Development 13: 139-141).
Another method for abolishing the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens. After generating the insertion mutants, the mutants can be screened to identify those containing the insertion in a transcription factor or transcription factor homologue gene. Plants containing a single transgene insertion event at the desired gene can be crossed to generate homozygous plants for the mutation (Koncz et al. (1992) Methods in Arabidopsis Research, World Scientific).
Alternatively, a plant phenotype can be altered by eliminating an endogenous gene, such as a transcription factor or transcription factor homologue, e.g., by homologous recombination (Kempin et al. (1997) Nature 389:802).
A plant trait can also be modified by using the cre-lox system (for example, as described in US Pat. No. 5,658,772). A plant genome can be modified to include first and second lox sites that are then contacted with a Cre recombinase. If the lox sites are in the same orientation, the intervening DNA sequence between the two sites is excised. If the lox sites are in the opposite orientation, the intervening sequence is inverted.
The polynucleotides and polypeptides of this invention can also be expressed in a plant in the absence of an expression cassette by manipulating the activity or expression level of the endogenous gene by other means. For example, by ectopically expressing a gene by T-DNA activation tagging (Ichikawa et al. (1997) Nature 390 698-701 ; Kakimoto et al. (1996) Science 274: 982-985). This method entails transforming a plant with a gene tag containing multiple transcriptional enhancers and once the tag has inserted into the genome, expression of a flanking gene coding sequence becomes deregulated. In another example, the transcriptional machinery in a plant can be modified so as to increase transcription levels of a polynucleotide of the invention (See, e.g., PCT Publications WO 96/06166 and WO 98/53057 which describe the modification of the DNA binding specificity of zinc finger proteins by changing particular amino acids in the DNA binding motif). The transgenic plant can also include the cellular machinery or mechanisms necessary for expressing or altering the activity of a polypeptide encoded by an endogenous gene, for example by altering the phosphorylation state of the polypeptide to maintain it in an activated state.
Transgenic plants (or plant cells, or plant explants, or plant tissues) incoφorating the polynucleotides of the invention and/or expressing the polypeptides of the invention can be produced by a variety of well established techniques as described above. Following construction of a vector, most typically an expression cassette, including a polynucleotide, e.g., encoding a transcription factor or transcription factor homologue, of the invention, standard techniques can be used to introduce the polynucleotide into a plant, a plant cell, a plant explant or a plant tissue of interest. Optionally, the plant cell, explant or tissue can be regenerated to produce a transgenic plant.
The plant can be any higher plant, including gymnosperms, monocotyledonous and dicotyledenous plants. Suitable protocols are available for Leguminosae (alfalfa, soybean, clover, etc.), Umbelliferae (carrot, celery, parsnip), Cruciferae (cabbage, radish, rapeseed, broccoli, etc.), Curcurhitaceae (melons and cucumber), Gramineae (wheat, com, rice, barley, millet, etc.), Solanaceae (potato, tomato, tobacco, peppers, etc.), and various other crops. See protocols described in Ammirato et al. (1984) Handbook of Plant Cell Culture -Crop Species. Macmillan Publ. Co. Shimamoto et al. (1989) Nature 338:274-276; Fromm et al. (1990) Bio/Technology 8:833-839; and Vasil et al. (1990) Bio/Technology 8:429-434. Transformation and regeneration of both monocotyledonous and dicotyledonous plant cells is now routine, and the selection of the most appropriate transformation technique will be determined by the practitioner. The choice of method will vary with the type of plant to be transformed; those skilled in the art will recognize the suitability of particular methods for given plant types. Suitable methods can include, but are not limited to: electroporation of plant protoplasts; liposome- mediated transformation; polyethylene glycol (PEG) mediated transformation; transformation using viruses; micro-injection of plant cells; micro-projectile bombardment of plant cells; vacuum infiltration; and Agrobacterium tumeficiens mediated transformation. Transformation means introducing a nucleotide sequence in a plant in a manner to cause stable or transient expression of the sequence.
Successful examples of the modification of plant characteristics by transformation with cloned sequences which serve to illustrate the current knowledge in this field of technology, and which are herein incoφorated by reference, include: U.S. Patent Nos. 5,571,706; 5,677,175; 5,510,471 ; 5,750,386; 5,597,945; 5,589,615; 5,750,871 ; 5,268,526; 5,780,708; 5,538,880; 5,773,269; 5,736,369 and 5,610,042. Following transformation, plants are preferably selected using a dominant selectable marker incoφorated into the transformation vector. Typically, such a marker will confer antibiotic or herbicide resistance on the transformed plants, and selection of transformants can be accomplished by exposing the plants to appropriate concentrations of the antibiotic or herbicide.
After transformed plants are selected and grown to maturity, those plants showing a modified trait are identified. The modified trait can be any of those traits described above. Additionally, to confirm that the modified trait is due to changes in expression levels or activity of the polypeptide or polynucleotide of the invention can be determined by analyzing mRNA expression using Northern blots, RT-PCR or microarrays, or protein expression using immunoblots or Western blots or gel shift assays.
INTEGRATED SYSTEMS— SEQUENCE IDENTITY
Additionally, the present invention may be an integrated system, computer or computer readable medium that comprises an instruction set for determining the identity of one or more sequences in a database. In addition, the instruction set can be used to generate or identify sequences that meet any specified criteria. Furthermore, the instruction set may be used to associate or link certain functional benefits, such improved flowering phenotype, with one or more identified sequence.
For example, the instruction set can include, e.g., a sequence comparison or other alignment program, e.g., an available program such as, for example, the Wisconsin Package Version 10.0, such as BLAST, FASTA, PILEUP, FINDPATTERNS or the like (GCG, Madison, WI). Public sequence databases such as GenBank, EMBL, Swiss-Prot and PIR or private sequence databases such as PhytoSeq (Incyte Pharmaceuticals, Palo Alto, CA) can be searched.
Alignment of sequences for comparison can be conducted by the local homology algorithm of Smith and Waterman (1981) Adv. Appl. Math. 2:482, by the homology alignment algorithm of Needleman and Wunsch (1970) J. Mol. Biol. 48:443, by the search for similarity method of Pearson and Lipman (1988) Proc. Natl. Acad. Sci. U.S.A. 85: 2444, by computerized implementations of these algorithms. After alignment, sequence comparisons between two (or more) polynucleotides or polypeptides are typically performed by comparing sequences of the two sequences over a comparison window to identify and compare local regions of sequence similarity. The comparison window can be a segment of at least about 20 contiguous positions, usually about 50 to about 200, more usually about 100 to about 150 contiguous positions. A description of the method is provided in Ausubel et al., supra. A variety of methods of determining sequence relationships can be used, including manual alignment and computer assisted sequence alignment and analysis. This later approach is a preferred approach in the present invention, due to the increased throughput afforded by computer assisted methods. As noted above, a variety of computer programs for performing sequence alignment are available, or can be produced by one of skill.
One example algorithm that is suitable for determining percent sequence identity and sequence similarity is the BLAST algorithm, which is described in Altschul et al. J. Mol. Biol 215:403-410 (1990). Software for performing BLAST analyses is publicly available, e.g., through the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always > 0) and N (penalty score for mismatching residues; always < 0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=- 4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff (1989) Proc. Natl. Acad. Sci. USA 89: 10915). In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g.. Karlin & Altschul (1993) Proc. Natl. Acad. Sci. USA 90:5873-5787). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence (and, therefore, in this context, homologous) if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.1, or less than about 0.01 , and or even less than about 0.001. An additional example of a useful sequence alignment algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pairwise alignments. The program can align, e.g., up to 300 sequences of a maximum length of 5,000 letters. The integrated system, or computer typically includes a user input interface allowing a user to selectively view one or more sequence records corresponding to the one or more character strings, as well as an instruction set which aligns the one or more character strings with each other or with an additional character string to identify one or more region of sequence similarity. The system may include a link of one or more character strings with a particular phenotype or gene function. Typically, the system includes a user readable output element which displays an alignment produced by the alignment instruction set.
The methods of this invention can be implemented in a localized or distributed computing environment. In a distributed environment, the methods may implemented on a single computer comprising multiple processors or on a multiplicity of computers. The computers can be linked, e.g. through a common bus, but more preferably the computer(s) are nodes on a network. The network can be a generalized or a dedicated local or wide-area network and, in certain preferred embodiments, the computers may be components of an intra-net or an internet.
Thus, the invention provides methods for identifying a sequence similar or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence. In the methods, a sequence database is provided (locally or across an inter or intra net) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.
Any sequence herein can be entered into the database, before or after querying the database. This provides for both expansion of the database and, if done before the querying step, for insertion of control sequences into the database. The control sequences can be detected by the query to ensure the general integrity of both the database and the query. As noted, the query can be performed using a web browser based interface. For example, the database can be a centralized public database such as those noted herein, and the querying can be done from a remote terminal or computer across an internet or intranet. EXAMPLES
The following examples are intended to illustrate but not limit the present invention.
EXAMPLE I. FULL LENGTH GENE IDENTIFICATION AND CLONING
Putative transcription factor sequences (genomic or ESTs) related to known transcription factors were identified in the Arabidopsis thaliana GenBank database using the tblastn sequence analysis program using default parameters and a P-value cutoff threshold of -4 or -5 or lower, depending on the length of the query sequence. Putative transcription factor sequence hits were then screened to identify those containing particular sequence strings. If the sequence hits contained such sequence strings, the sequences were confirmed as transcription factors.
Alternatively, Arabidopsis thaliana cDNA libraries derived from different tissues or treatments, or genomic libraries were screened to identify novel members of a transcription family using a low stringency hybridization approach. Probes were synthesized using gene specific primers in a standard PCR reaction
(annealing temperature 60° C) and labeled with 2P dCTP using the High Prime DNA Labeling Kit (Boehringer Mannheim). Purified radiolabelled probes were added to filters immersed in Church hybridization medium (0.5 M NaPO4 pH 7.0, 7% SDS, 1 % w/v bovine serum albumin) and hybridized overnight at 60 °C with shaking. Filters were washed two times for 45 to 60 minutes with lxSCC, 1% SDS at 60° C.
To identify additional sequence 5' or 3' of a partial cDNA sequence in a cDNA library, 5' and 3' rapid amplification of cDNA ends (RACE) was performed using the Marathon™ cDNA amplification kit (Clontech, Palo Alto, CA). Generally, the method entailed first isolating poly(A) mRNA, performing first and second strand cDNA synthesis to generate double stranded cDNA, blunting cDNA ends, followed . by ligation of the Marathon ΓM Adaptor to the cDNA to form a library of adaptor- ligated ds cDNA.
Gene-specific primers were designed to be used along with adaptor specific primers for both 5' and 3' RACE reactions. Nested primers, rather than single primers, were used to increase PCR specificity. Using 5' and 3' RACE reactions, 5' and 3 ' RACE fragments were obtained, sequenced and cloned. The process can be repeated until 5' and 3' ends of the full-length gene were identified. Then the full- length cDNA was generated by PCR using primers specific to 5' and 3' ends of the gene by end-to-end PCR.
EXAMPLE II. CONSTRUCTION OF EXPRESSION VECTORS
The sequence was amplified from a genomic or cDNA library using primers specific to sequences upstream and downstream of the coding region. The expression vector was pMEN20 or pMEN65, which are both derived from pMON316 (Sanders et al, (1987) Nucleic Acids Research 15:1543-58) and contain the CaMV 35S promoter to express transgenes. To clone the sequence into the vector, both pMEN20 and the amplified DNA fragment were digested separately with Sail and Notl restriction enzymes at 37° C for 2 hours. The digestion products were subject to electrophoresis in a 0.8% agarose gel and visualized by ethidium bromide staining. The DNA fragments containing the sequence and the linearized plasmid were excised and purified by using a Qiaquick gel extraction kit (Qiagen, CA). The fragments of interest were ligated at a ratio of 3: 1 (vector to insert). Ligation reactions using T4 DNA ligase (New England Biolabs, MA) were carried out at 16° C for 16 hours. The ligated DNAs were transformed into competent cells of the E. coli strain DH5alpha by using the heat shock method. The transformations were plated on LB plates containing 50 mg/1 kanamycin (Sigma). Individual colonies were grown overnight in five milliliters of LB broth containing 50 mg/1 kanamycin at 37° C. Plasmid DNA was purified by using Qiaquick Mini Prep kits (Qiagen, CA).
EXAMPLE III. TRANSFORMATION OF AGROBACTERIUM WITH THE EXPRESSION VECTOR
After the plasmid vector containing the gene was constmcted, the vector was used to transform Agrobacterium tumefaciens cells expressing the gene products. The stock of Agrobacterium tumefaciens cells for transformation were made as described by Nagel et al. (1990) FEMS Microbiol Letts. 67: 325-328. Agrobacterium strain ABI was grown in 250 ml LB medium (Sigma) overnight at 28°C with shaking until an absorbance (A6oo) of 0.5 - 1.0 was reached. Cells were harvested by centrifugation at 4,000 x g for 15 min at 4° C. Cells were then resuspended in 250 μl chilled buffer (1 mM HEPES, pH adjusted to 7.0 with KOH). Cells were centrifuged again as described above and resuspended in 125 μl chilled buffer. Cells were then centrifuged and resuspended two more times in the same HEPES buffer as described above at a volume of 100 μl and 750 μl, respectively. Resuspended cells were then distributed into 40 μl aliquots, quickly frozen in liquid nitrogen, and stored at -80° C.
Agrobacterium cells were transformed with plasmids prepared as described above following the protocol described by Nagel et al. For each DNA construct to be transformed, 50 - 100 ng DNA (generally resuspended in 10 mM Tris- HCl, 1 mM EDTA, pH 8.0) was mixed with 40 μl of Agrobacterium cells. The DNA/cell mixture was then transferred to a chilled cuvette with a 2mm electrode gap and subject to a 2.5 kV charge dissipated at 25 μF and 200 μF using a Gene Pulser II apparatus (Bio-Rad). After electroporation, cells were immediately resuspended in 1.0 ml LB and allowed to recover without antibiotic selection for 2 - 4 hours at 28° C in a shaking incubator. After recovery, cells were plated onto selective medium of LB broth containing 100 μg/ml spectinomycin (Sigma) and incubated for 24-48 hours at 28° C. Single colonies were then picked and inoculated in fresh medium. The presence of the plasmid construct was verified by PCR amplification and sequence analysis.
EXAMPLE IV. TRANSFORMATION OF ARABIDOPSIS PLANTS WITH AGROBACTERIUM TUMEFACIENS WITH EXPRESSION VECTOR
After transformation of Agrobacterium tumefaciens with plasmid vectors containing the gene, single Agrobacterium colonies were identified, propagated, and used to transform Arabidopsis plants. Briefly, 500 ml cultures of LB medium containing 50 mg/1 kanamycin were inoculated with the colonies and grown at 28° C with shaking for 2 days until an absorbance (Aήoo) of > 2.0 is reached. Cells were then harvested by centrifugation at 4,000 x g for 10 min, and resuspended in infiltration medium (1/2 X Murashige and Skoog salts (Sigma), 1 X Gamborg's B-5 vitamins (Sigma), 5.0% (w/v) sucrose (Sigma), 0.044 μM benzylamino purine (Sigma), 200 μl/L Silwet L-77 (Lehle Seeds) until an absorbance (A60o) of 0.8 was reached.
Prior to transformation, Arabidopsis thaliana seeds (ecotype Columbia) were sown at a density of -10 plants per 4" pot onto Pro-Mix BX potting medium (Hummert International) covered with fiberglass mesh (18 mm X 16 mm). Plants were grown under continuous illumination (50-75 μE/m2/sec) at 22-23° C with 65-70% relative humidity. After about 4 weeks, primary inflorescence stems (bolts) are cut off to encourage growth of multiple secondary bolts. After flowering of the mature secondary bolts, plants were prepared for transformation by removal of all siliques and opened flowers.
The pots were then immersed upside down in the mixture of Agrobacterium infiltration medium as described above for 30 sec, and placed on their sides to allow draining into a 1 ' x 2' flat surface covered with plastic wrap. After 24 h, the plastic wrap was removed and pots are turned upright. The immersion procedure was repeated one week later, for a total of two immersions per pot. Seeds were then collected from each transformation pot and analyzed following the protocol described below.
EXAMPLE V. IDENTIFICATION OF ARABIDOPSIS PRIMARY TRANSFORMANTS
Seeds collected from the transformation pots were sterilized essentially as follows. Seeds were dispersed into in a solution containing 0.1% (v/v) Triton X- 100 (Sigma) and sterile H 0 and washed by shaking the suspension for 20 min. The wash solution was then drained and replaced with fresh wash solution to wash the seeds for 20 min with shaking. After removal of the second wash solution, a solution containing 0.1% (v/v) Triton X-100 and 70% ethanol (Equistar) was added to the seeds and the suspension was shaken for 5 min. After removal of the ethanol/detergent solution, a solution containing 0.1% (v/v) Triton X-100 and 30% (v/v) bleach (Clorox) was added to the seeds, and the suspension was shaken for 10 min. After removal of the bleach/detergent solution, seeds were then washed five times in sterile distilled H20. The seeds were stored in the last wash water at 4° C for 2 days in the dark before being plated onto antibiotic selection medium (1 X Murashige and Skoog salts (pH adjusted to 5.7 with IM KOH), 1 X Gamborg's B-5 vitamins, 0.9% phytagar (Life Technologies), and 50 mg/1 kanamycin). Seeds were germinated under continuous illumination (50-75 μE/m2/sec) at 22-23° C. After 7-10 days of growth under these conditions, kanamycin resistant primary transformants (Ti generation) were visible and obtained. These seedlings were transferred first to fresh selection plates where the seedlings continued to grow for 3-5 more days, and then to soil (Pro-Mix BX potting medium).
Primary transformants were crossed and progeny seeds (T2) collected; kanamycin resistant seedlings were selected and analyzed. The expression levels of the recombinant polynucleotides in the transformants varies from about a 5% expression level increase to a least a 100% expression level increase. Similar observations are made with respect to polypeptide level expression.
EXAMPLE VI. IDENTIFICATION OF ARABIDOPSIS PLANTS WITH TRANSCRIPTION FACTOR GENE KNOCKOUTS
The screening of insertion mutagenized Arabidopsis collections for null mutants in a known target gene was essentially as described in Krysan et al ( 1999) Plant Cell 1 1 :2283-2290. Briefly, gene-specific primers, nested by 5-250 base pairs to each other, were designed from the 5 ' and 3 ' regions of a known target gene. Similarly, nested sets of primers were also created specific to each of the T- DNA or transposon ends (the "right" and "left" borders). All possible combinations of gene specific and T-DNA/transposon primers were used to detect by PCR an insertion event within or close to the target gene. The amplified DNA fragments were then sequenced which allows the precise determination of the T-DNA/transposon insertion point relative to the target gene. Insertion events within the coding or intervening sequence of the genes were deconvoluted from a pool comprising a plurality of insertion events to a single unique mutant plant for functional characterization. The method is described in more detail in Yu and Adam, US Application Serial No. 09/177,733 filed October 23, 1998. EXAMPLE VII. IDENTIFICATION OF MODIFIED FLOWERING TIME OR FLOWERING PERIOD IN OVEREXPRESSOR OR GENE KNOCKOUT PLANTS
Experiments were performed to identify those transformants or knockouts that exhibited a modified flowering time or flowering period phenotype. For such studies, the transformants were observed for modified phenotypes over the period from seedling growth to senescence. The plants were grown under continuous light conditions at 20-25° C.
We observed that plants overexpressing G2010 (SEQ ID Nos. 7 and 8) constitutively (three independent T2 populations having 6 plants in one population and 16 plants in each of the other two) flowered approximately 1 week earlier than control plants transformed with an empty transformation vector under the control of the 35S promoter. The primary shoot of 35S::G2010 plants produced 5-6 rosette leaves before bolting, compared to 8-10 rosette leaves in controls. Flower buds were first visible 12-14 days after sowing in 35S::G2010 plants compared with approximately 20 days for wild type. At 20 days the 35S::G2010 plants have open flowers at this time whereas the wild type has yet to generate an inflorescence.
G2010 is a member of the SBP family of transcription factors and corresponds to spl4 (Cardon et al. (1999) Gene 237:91-104). Expression of spl4 is upregulated during development under both long day and short day conditions and spl4 is highly expressed in the inflorescence tissue. Expression of G2010 is localized to the rib meristem and inter-primordial regions of the inflorescence apex (Cardon et al. (1999) Gene 237:91-104).
The utility of a gene such as G2010 that functions to accelerate flowering includes improving modem crop varieties, for example. Most modern crop varieties are the result of extensive breeding programs. Many generations of backcrossing may be required to introduce desired traits. Systems that accelerate flowering could have valuable applications in such programs since they allow much faster generation times. Additionally, in some instances, a faster generation time might allow additional harvests of a crop to be made within a given growing season. We identified an additional gene that is related based on sequence identity and therefore may also be suitable for creating an early flowering phenotype. The gene is G2347 (SEQ ID NO: 19 and 20). G2347 shares about 52% sequence identity over the whole sequence length and 95% sequence identity over the conserved domain.
We also observed that a population of homozygous plants which carried a T-DNA insertion in the coding region of G1037 (SEQ ID NO: 9 and 10) had a modified flowering phenotype. Knockout G1037 plants (12 individuals in total), grown under continuous light conditions at 20-25° C, produced 4-7 primary rosette leaves before bolting compared to 8-9 rosette leaves in controls harboring an empty transformation vector. Flower buds were first visible in knockout G1037 plants approximately 1 week earlier than in controls. Early flowering was also noted in knockout G 1037 plants grown for 1 week in continuous light followed by subsequent growth under 12 hours light. At 28 days the flower buds are visible in the knockout G1037 but not the controls.
We also identified additional genes that are related based on sequence identity and therefore may also be suitable for observing an early flowering phenotype. The gene is G722 (SEQ ID NO: 21 and 22) and G1493 (SEQ ID NO: 23 and 24). G722 shares about 66% sequence identity over the whole sequence length compared with G1037 and 78% sequence identity over the conserved domain compared with G1037. G1493 shares about 40% sequence identity over the whole sequence length and 78% sequence identity over the conserved domain. Further, we identified additional overexpressor plants that also had a modified flowering phenotype. Table 5 shows the phenotypes observed for particular overexpressor or knockout plants and provides the SEQ ID No., the internal reference code (GID), whether a knockout or overexpressor plant was analyzed and the observed phenotype.
Table 5
Figure imgf000052_0001
Gl 52 (SEQ ID NO: 13 and 14), G153 (SEQ ID NO: 15 and 16), and G860 (SEQ ID NO: 17 and 18) were all found to be related to G1760 (SEQ ID NO: 3 and 4). Gl 52 shares about 75% sequence identity over the whole sequence length and 93% sequence identity over the conserved domain. G153 shares about 60% sequence identity over the whole sequence length and 85% sequence identity over the conserved domain. G860 shares about 61% sequence identity over the whole sequence length and 85% sequence identity over the conserved domain.
Another knockout G1947 (SEQ ID NO: 11 and 12) showed an extended flowering period or extended reproductive phase. A control plant population produced flowers for approximately 15 days whereas the overexpressor plant population flowered for approximately 30 days due to a longer retention period of the flowers or a delay in senescense.
By altering the expression levels of the genes of the present invention a variety of plant traits can be altered. For example, plants with accelerated, delayed, or inducible flowering times may be generated. Alternatively the vernalization period or flower retention period or an increase in the total number of flowers may be achieved. (1) Accelerated flowering
A number of Arabidopsis genes have already been shown to accelerate flowering when constitutively expressed. These include LEAFY, APETALA1 and CONSTANS. In these cases, however, the early flowering plants showed undesirable side effects such as extreme dwarfing, infertility, or premature termination of shoot meristem growth (Mandel, M. et al., 1995, Nature 377, 522-524; Weigel, D. and Nilsson, O., 1995, Nature 377, 495-500; Simon et al., 1996, Nature 384, 59-62, Onouchi et al., 2000, Plant Cell 12, 885-900). Systems that accelerate flowering could have valuable applications in modem crop production programs since they allow much faster generation times. Additionally, in some instances, a faster generation time might allow additional harvests of a crop to be made within a given growing season.
With the advent of transformation systems for tree species such as oil palm and Eucalyptus, forest biotechnology is a growing area of interest. Acceleration of flowering, again, might reduce generation times and make breeding programs feasible which would otherwise be impossible. That this is a real possibility has already been demonstrated in aspen, a tree species that usually takes 8-20 years to flower. Transgenic aspen that overexpress the Arabidopsis LFY gene flower after only 5 months. The flowers produced by these young aspen plants, however, were sterile; the challenge of producing fertile early flowering trees therefore still remains (Weigel, D. and Nilsson, O, 1995, Nature 377, 495-500).
(2) Delayed Flowering
In species such as sugarbeet where the vegetative parts of the plants constitute the crop and the reproductive tissues are discarded, it would be advantageous to delay or prevent flowering. Extending vegetative development could bring about large increases in yields.
Additionally, a major concern is the escape of transgenic pollen from GMOs to wild species or so-called organic crops. Systems that prevent vegetative transgenic crops from flowering would eliminate this worry.
(3) Inducible Flowering
By regulating the expression of genes of the invention in transgenic plants using inducible promoters, flowering could be triggered by application of an inducer chemical. This would allow flowering to be synchronized across a crop and facilitate more efficient harvesting. Such inducible systems could be used to tune the flowering of crop varieties to different latitudes. At present, species such as soybean and cotton are available as a series of maturity groups that are suitable for different latitudes on the basis of their flowering time (which is governed by day-length). A system in which flowering could be chemically controlled would allow a single high-yielding Northern maturity group to be grown at any latitude. In Southern regions such plants could be grown for longer, thereby increasing yields, before flowering was induced. In more Northern areas, the induction would be used to ensure that the crop flowers prior to the first winter frosts.
Currently, the existence of a series of maturity groups for different latitudes represents a major barrier to the introduction of new valuable traits. Any trait (e.g. disease resistance) has to be bred into each of the different maturity groups separately; a laborious and costly exercise. The availability of single strain, which could be grown at any latitude, would therefore greatly increase the potential for introducing new traits to crop species such as soybean and cotton.
An application might also exist in fruit trees such as apple, in which flowering shoots lie dormant for long periods, before flowers develop. Genes which accelerate flowering might be applied to synchronized flower development and break this dormancy.
(4) Vernalization
Specific applications for some of these genes relate to their potential roles in the vernalization response. For many crop species, high yielding winter-varieties can only be grown in temperate regions where the winter season is prolonged and cold enough to elicit a vernalization response. Constitutive expression may compensate for a vernalization treatment in late-flowering ecotypes. Winter varieties of wheat, for instance, which over-express a gene (or the wheat ortholog) might then be grown in areas like Southern California which would otherwise be too warm to allow effective vernalization. A second application for this system is in cherry (Prunus). Locally grown cherries are unavailable in the early Californian spring since the winters are too warm for vernalization to occur.
Genes or sequences selected from Table 1 or Table 2 can also be overexpressed or knocked out in a plant to produce a plant with modified flowering trait. Preferably, the sequence selected (Test Sequence) from Table 1 or Table 2 is overexpressed in the same species listed for the selected sequences, however, another species may be used.
EXAMPLE VIII. IDENTIFICATION OF HOMOLOGOUS SEQUENCES
Homologous sequences from Arabidopsis and plant species other than
Arabidopsis are identified using database sequence search tools, such as the Basic Local Alignment Search Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215:403- 410; and Altschul et al. (1997) Nucl. Acid Res. 25: 3389-3402). The tblastx sequence analysis programs are employed using the BLOSUM-62 scoring matrix (Henikoff, S. and Henikoff, J. G. (1992) Proc. Natl. Acad. Sci. USA 89: 10915-10919). Identified Arabidopsis homologous sequences are provided in Tables 1 and 2, appended to this application. These sequences can be inserted into a plant to modify a flowering trait as shown above. The percent sequence identity among these sequences can be as low as 47%, or even lower sequence identity. The entire NCBI GenBank database was filtered for sequences from all plants except Arabidopsis thaliana by selecting all entries in the NCBI GenBank database associated with NCBI taxonomic ID 33090 (Viridiplantae; all plants) and excluding entries associated with taxonomic ID 3701 (Arabidopsis thaliana). These sequences were compared to sequences representing genes of SEQ ID NO: 1-26 using the Washington University TBLASTX algorithm (version 2.0al9MP) at the default settings using gapped alignments with the filter "off." For each gene of SEQ ID NO: 1 -26, individual comparisons are ordered by probability score (P-value), where the score reflects the probability that a particular alignment occurred by chance. For example, a score of 3.6e-40 is 3.6 x 10-40. In addition to P-values, comparisons are also scored by percentage identity. Percentage identity reflects the degree to which two segments of DNA or protein are identical over a particular length.
Table 1 (appended to this application) lists a summary of orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search. The first column shows the polynucleotide sequence identifier (SEQ ID NO), the second column shows the transcription factor cDNA identifier (Gene ID), the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide sequence identified in a BLAST search (Test Sequence ID), the fourth column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability), the fifth column identifies the plant species of the Test Sequence (Test Sequence Species), and the sixth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation). Table 2 (appended to this application) lists orthologous and homologous sequences identified using BLAST (tblastx program) and the standard BLAST result data generated from a search. The first column shows the polynucleotide sequence identifier (SEQ ID NO), the second column shows the transcription factor cDNA identifier (Gene ID), the third column shows the GenBank Accession Number of the orthologous or homologous polynucleotide (Test Sequence ID), the fourth column shows the GenBank annotation for the sequence identified in a BLAST search (Test Sequence GenBank Annotation), the fifth column shows the reading frame of the Test sequence encoding the orthologous or homologous sequence (Reading Frame), the sixth column shows the calculated score value of the aligned sequences (High Score), the seventh column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability), and the eighth column shows the number of regions in the Test Sequence that align with a sequence from the SEQ ID NO. (N).
All references, publications, patents and other documents and information sources noted herein are incoφorated by reference in their entirety for all puφoses. Although the invention has been described with reference to the embodiments and examples above, it should be understood that various modifications can be made without departing from the spirit of the invention.
Table 1
SEQ ID Gene ID Test Smallest Test Sequence Species Test Sequence GenBank Annotation
NO Sequence ID Sum Probability
G590 AW782148 8.60E-29 [Glycine max] sm02b05.y1 Gm-c1027 Glycine max cDNA clone GENO
G590 BH708336 6.10E-17 [Brassica oleracea] BOMKN39TF BO_2_3_KB Brassica oleracea gen
G590 AQ291034 1.30E-14 [Oryza sativa] nbxb0038K13r CUGI Rice BAC Library Oryza sativ
G590 AI484932 1.40E-13 Lycopersicon esculentum] EST243195 tomato ovary, TAMU Lycope
G590 BI308330 1.60E-08 [Medicago truncatula] EST529740 GPOD Medicago truncatula cDNA
G590 BM408345 5.90E-08 [Solanum tuberosum] EST582672 potato roots Solanum tuberosum
G590 BG525606 0.00033 [Stevia rebaudiana] 51-65 Stevia field grown leaf cDNA Stevia
G590 BI070102 0.024 [Populus tremula x Populus tremuloides] C014P11 U Populus stra
G590 BG052163 0.025 [Sorghum propinquum] RHIZ2_6_H10.b1_A003 Rhizome2 (RHIZ2) Sor
G590 BM323123 0.085 [Sorghum bicolor] PIC1_8_C08.b1_A002 Pathogen-infected compat
G590 gi15451582 6.80E-16 [Oryza sativa] Putative SPATULA.
G590 gi6166283 0.18 [Pinus taeda] helix-loop-helix protein 1A.
G590 gi7248461 0.88 [Zea mays] root cap-specific protein.
G590 gi5923912 0.95 Tulipa gesneriana] bHLH transcription factor GBOF-1.
G590 gi13346180 0.97 [Gossypium hirsutum] GHDEL61.
G590 gi21122 0.99 [Raphanus sativus] seed protein (AA 1-83).
G590 gi1086538 1 [Oryza rufipogon] transcriptional activator Rb homolog.
G590 gi527657 1 [Pennisetum glaucum] myc-like regulatory R gene product.
G590 gi3127045 1 [Petunia x hybrida] bHLH transcription factor JAF13.
G590 gi5880765 1 [Chloroplast Nephroselmis olivacea] ChlB subunit of protoch
3 G1760 AMDEFH125 8.40E-37 [Antirrhinum majus] A.majus mRNA for DEFH125 protein.
3 G1760 AW218280 4.40E-36 Lycopersicon esculentum] EST303461 tomato radicle, 5 d post-
3 G1760 AI974336 5.70E-36 [Medicago truncatula] T110185e KV0 Medicago truncatula cDNA c
3 G1760 OSJN00003 5.70E-36 [Oryza sativa] chromosome 4 clone OSJNBa0072F16, *" SEQUENC
3 G1760 AP004766 2.20E-35 Oryza sativa japonica cultivar-group] chromosome 2 clone B10
3 G1760 BG592879 2.20E-32 [Solanum tuberosum] EST491557 cSTS Solanum tuberosum cDNA do
3 G1760 AX085162 6.80E-31 [Zea mays] Sequence 12 from Patent WO0112798.
3 G1760 AW706936 2.70E-27 Glycine max] sk08d08.y1 Gm-c1023 Glycine max cDNA clone GENO
3 G1760 AP004526 4.60E-27 Lotus japonicus] genomic DNA, chromosome 6, clone:LjT05E07,
3 G1760 BE596704 7.10E-27 [Sorghum bicolor] PI1_58_F04.b1_A002 Pathogen induced 1 (PI1 )
Table 1
G1760 gi 1928874 4.50E-48 [Medicago sativa] MADS-box protein.
G1760 gi1816459 3.30E-47 [Antirrhinum majus] DEFH125 protein.
G1760 gi9964296 2.50E-44 [Zea mays] MADS box protein 2.
G1760 gi15081463 8.40E-37 [Ipomoea batatas] MADS-box protein.
G1760 gi1568513 1.70E-27 [Petunia x hybrida] fbp11.
G1760 gi1914838 3.50E-27 [Oryza sativa] MADS box protein.
G1760 gi861081 1.20E-26 [Panax ginseng] agamous.
G1760 gi16973298 3.00E-26 [Malus x domestica] C-type MADS box protein.
G1760 gi6840998 4.30E-26 [Pinus radiata] DEF/GLO-like protein.
G1760 gi602900 4.80E-26 [Silene latifolia] SLM1.
G1820 AW776719 2.20E-41 [Medicago truncatula] EST335784 DSIL Medicago truncatula cDNA
G1820 BG591677 3.90E-38 [Solanum tuberosum] EST499519 P. infestans-challenged leaf So
G1820 BI701620 1.20E-36 [Glycine max] sai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN
G1820 BH725354 1.40E-35 [Brassica oleracea] BOHV037TF BO_2_3_KB Brassica oleracea gen
G1820 AW093662 4.80E-34 [Lycopersicon esculentum] EST286842 tomato mixed elicitor, BT
G1820 BE208917 5.60E-34 [Citrus x paradisi] GF-FV-P3F5 Marsh grapefruit young flavedo
G1820 BG440805 1.50E-33 [Gossypium arboreum] GA Ea0010D12f Gossypium arboreum 7-10 d
G1820 BF473402 1.10E-32 [Triticum aestivum] WHE0924_A08_B16ZS Wheat 5-15 DAP spike cD
G1820 BM816719 1.40E-32 [Hordeum vulgare] HB02A06_T3.ab1 HB Hordeum vulgare cDNA clon
G1820 AP004113 3.50E-32 [Oryza sativa] chromosome 2 clone OJ1116_A06, *" SEQUENCING
G1820 gi5257260 6.50E-34 [Oryza sativa] Similar to sequence of BAC F7G19 from Arabid
G1820 gi18481626 3.00E-07 [Zea mays] repressor protein.
G1820 gi297871 0.9 [Picea abies] histone H2A.
G1820 gi297887 0.92 [Daucus carota] glycine rich protein.
G1820 gi 13774274 0.94 [Poa secunda] metallothionein-like protein type 2.
G1820 gi536892 0.98 [Triticum aestivum] protein H2A.
G1820 gi6782438 1 [Nicotiana glauca] glycine-rich protein.
G1820 gi1087017 1 [Nicotiana alata] arabinogalactan-protein, AGP [Nicotiana a
G2010 BI931517 2.60E-31 [Lycopersicon esculentum] EST551406 tomato flower, 8 mm to pr
G2010 BG525285 3.20E-31 [Stevia rebaudiana] 48-3 Stevia field grown leaf cDNA Stevia
G2010 BE058432 1.30E-29 [Glycine max] sn16a06.y1 Gm-d 016 Glycine max cDNA clone GENO
G2010 AMSPB1 1.60E-29 [Antirrhinum majus] A.majus mRNA for squamosa-promoter bindin
G2010 BG455868 3.10E-29 [Medicago truncatula] NF068F05PL1 F1045 Phosphate starved leaf
Table 1
G2010 BI071295 6.20E-28 [Populus tremula x Populus tremuloide C054P79U Populus stra
G2010 BE499739 6.90E-27 [Triticum aestivum] WHE0975_G04_M07ZS Wheat pre-anthesis spik
G2010 BE060783 9.80E-27 [Hordeum vulgare] HVSMEg0013F15f Hordeum vulgare pre-anthesis
G2010 BG097153 3.10E-25 [Solanum tuberosum] EST461672 potato leaves and petioles Sola
G2010 AW747167 1.30E-24 [Sorghum bicolor] WS1_66_F11.b1_A002 Water-stressed 1 (WS1 ) S
G2010 gi1183866 9.90E-32 [Antirrhinum majus] squamosa-promoter binding protein 1.
G2010 gi5931780 5.50E-25 [Zea mays] SBP-domain protein 2.
G2010 gi8468036 1.60E-20 [Oryza sativa] Similar to Arabidopsis thaliana chromosome 2
G2010 gi9087308 7.40E-10 [Mitochondrion Beta vulgaris var. altiss orf102a.
G1037 BH007675 1.60E-80 [Brassica oleracea] ee72e06.x1 TO1000 Brassica oleracea genom
G1037 BM407041 1.80E-65 [Solanum tuberosum] EST581368 potato roots Solanum tuberosum
G1037 AB062095 1.00E-61 [Zea mays] ZmRR9 mRNA for response regulator 9, complete eds.
G1037 AP004094 4.10E-58 [Oryza sativa] chromosome 2 clone OJ1695JH09, *" SEQUENCING
G1037 BG450692 1.00E-51 [Medicago truncatula] NF102C07DT1F1052 Drought Medicago trunc
G1037 AW564570 4.30E-42 [Sorghum bicolor] LG1_296_E01.b1_A002 Light Grown 1 (LG1) Sor
G1037 BG523436 9.30E-41 [Stevia rebaudiana] 31-73 Stevia field grown leaf cDNA Stevia
G1037 AW596288 1.00E-38 [Glycine max] sj01 e07.y1 Gm-c1032 Glycine max cDNA clone GENO
G1037 BJ 181458 4.10E-34 [Physcomitrella patens subsp. patens] BJ 181458 normalized ful
G1037 BE214029 2.10E-31 [Hordeum vulgare] HV_CEb0001 P06f Hordeum vulgare seedling gre
G1037 gi14189890 2.20E-64 [Zea mays] response regulator 9.
G1037 gi 15289981 5.90E-09 [Oryza sativa] hypothetical protein.
G1037 gi 1076557 0.0072 [Vigna unguiculata] extensin-like protein - cowpea (fragmen
G1037 gi13173408 0.0076 [Dianthus caryophyllus] response regulator protein.
G1037 gi17227037 0.32 [Solanum chacoense] style-specific self-incompatibility put
G1037 gi4566516 0.37 [Chlamydomonas reinhardtii] gamete-specific homeodomain pro
G1037 gi14626761 0.88 [Lycopersicon esculentum] mature anther-specific protein LA
G1037 gi19925 0.89 [Nicotiana tabacum] pistil extensin like protein, partial
G1037 gi4106378 1 [Brassica napus] calcium-binding protein.
G1947 AP003826 6.80E-45 [Oryza sativa] chromosome 7 clone OJ1361_E02, *** SEQUENCING
G1947 BE319312 1.20E-43 [Medicago truncatula] NF015D08NR1 F1035 Nodulated root Medicag
G1947 BI406849 3.90E-43 [Solanum tuberosum] 182A06 Mature tuber lambda ZAP Solanum tu
G1947 LPHSF30 4.20E-42 [Lycopersicon peruvianum] L.peruvianum Lp-hsf30 mRNA for heat
Table 1
G1947 BM086093 8.60E-42 [Glycine max] sah35d07.y1 Gm-c1036 Glycine max cDNA clone SOY
G1947 AV833112 1.50E-41 [Hordeum vulgare subsp. vulgare] AV833112 K. Sato unpublished
G1947 AW036683 2.30E-41 [Lycopersicon esculentum] EST278726 tomato fruit mature green
G1947 BI479783 4.60E-34 Triticum aestivum] WHE3452_A08_A16ZS Wheat pre-anthesis spik
G1947 BM373839 8.90E-34 [Hordeum vulgare] EBma03_SQ002_N22_R IGF Barley EBma03 librar
G1947 BH535815 4.20E-33 [Brassica oleracea] BOGOA14TR BOGO Brassica oleracea genomic
G1947 gi 19490 5.90E-45 [Lycopersicon peruvianum] heat stress transcription factor
G1947 gi662924 2.20E-37 [Glycine max] heat shock transcription factor 21.
G1947 gi2130133 1.60E-33 [Zea mays] heat shock transcription factor (clone hsfa) - m
G1947 gi19260 4.20E-33 [Lycopersicon esculentum] heat stress transcription factor
G1947 gi16118447 2.40E-32 Phaseolus acutifolius] heat shock transcription factor.
G1947 gi5821138 1.00E-30 Nicotiana tabacum] heat shock factor.
G1947 gi7158882 8.70E-30 Medicago sativa] heat shock transcription factor.
G1947 gi3550552 2.00E-29 Pisum sativum] heat shock transcription factor (HSFA).
G1947 gi14209551 2.40E-29 Oryza sativa] putative heat shock factor.
G1947 gi1155054 0.064 [Phaseolus vulgaris] regulator of MAT2.
G152 AP004766 2.10E-28 Oryza sativa japonica cultivar-group] chromosome 2 clone B10
G152 OSJN00003 1.00E-27 Oryza sativa] chromosome 4 clone OSJNBa0072F16, *** SEQUENC
G152 AX085151 1.30E-27 [Zea mays] Sequence 1 from Patent WO0112798.
G152 AMDEFH125 2.80E-26 Antirrhinum majus] A.majus mRNA for DEFH125 protein.
G152 BM405213 7.60E-26 [Solanum tuberosum] EST579540 potato roots Solanum tuberosum
G152 AI974336 1.80E-25 Medicago truncatula] T110185e KV0 Medicago truncatula cDNA c
G152 AW218280 2.90E-25 Lycopersicon esculentum] EST303461 tomato radicle, 5 d post-
G152 AW706936 1.00E-24 Glycine max] sk08d08.y1 Gm-c1023 Glycine max cDNA clone GENO
G152 AP004526 8.40E-24 Lotus japonicus] genomic DNA, chromosome 6, clone:LjT05E07,
G152 MSU91964 1.50E-23 Medicago sativa] MADS-box protein mRNA, partial eds.
G152 gi9964296 9.80E-47 Zea mays] MADS box protein 2.
G152 gi1816459 4.60E-42 Antirrhinum majus] DEFH125 protein.
G152 gi15081463 7.40E-40 Ipomoea batatas] MADS-box protein.
G152 gi 1928874 2.00E-35 Medicago sativa] MADS-box protein.
G152 gi 18996775 1.10E-24 Helianthus annuus] MADS-box transcription factor HAM75.
G152 gi4103342 1.90E-24 Cucumis sativus] agamous-like putative transcription facto
G152 gi602906 4.50E-24 Silene latifolia] SLM4.
Table 1
G152 gi4218162 4.80E-24 [Gerbera hybrida] MADS-box protein, GAGA2.
G152 gi167126 6.30E-24 [Brassica napus] BAG1.
G152 gi 10946429 6.70E-24 [Eucalyptus globulus] MADS-box protein EAP1.
G153 AMDEFH125 1.20E-36 [Antirrhinum majus] A.majus mRNA for DEFH125 protein.
G153 AW706936 1.50E-35 [Glycine max] Sk08d08.y1 Gm-c1023 Glycine max cDNA clone GENO
G153 AP004526 1.20E-30 [Lotus japonicus] genomic DNA, chromosome 6, clone:LjT05E07,
G153 AW218280 6.60E-30 [Lycopersicon esculentum] EST303461 tomato radicle, 5 d post-
G153 BM405213 9.30E-28 [Solanum tuberosum] EST579540 potato roots Solanum tuberosum
G153 AI974336 1.50E-26 [Medicago truncatula] T110185e KVO Medicago truncatula cDNA c
G153 AX085151 9.70E-25 [Zea mays] Sequence 1 from Patent WO0112798.
G153 MSU91964 1.00E-24 Medicago sativa] MADS-box protein mRNA, partial eds.
G153 AP003868 7.20E-22 [Oryza sativa] chromosome 8 clone OJ1111_B08, *" SEQUENCING
G153 BE034403 2.70E-20 Mesembryanthemum crystallinum] MH04D03 MH Mesembryanthemum c
G153 gi1816459 1.10E-42 [Antirrhinum majus] DEFH125 protein.
G153 gi9964296 5.40E-42 Zea mays] MADS box protein 2.
G153 gi1928874 2.70E-34 [Medicago sativa] MADS-box protein.
G153 gi15824795 9.90E-31 [Oryza sativa] MADS-box protein FDRMADS3.
G153 gi15081463 1.80E-28 [Ipomoea batatas] MADS-box protein.
G153 gi4204232 2.10E-26 Lolium temulentum] MADS-box protein 1.
G153 gi167126 2.90E-26 [Brassica napus] BAG1.
G153 gi13446154 5.90E-26 [Pisum sativum] MADS-box transcription factor.
G153 gi9367313 8.70E-26 Hordeum vulgare] MADS-box protein 8.
G153 gi6634708 1.10E-25 Petunia x hybrida] MADS box transcription factor.
G860 AI974336 1.20E-37 [Medicago truncatula] T110185e KVO Medicago truncatula cDNA c
G860 AMDEFH125 1.30E-37 Antirrhinum majus] A.majus mRNA for DEFH125 protein.
G860 AX085151 5.00E-37 Zea mays] Sequence 1 from Patent WO0112798.
G860 OSJN00003 3.90E-36 Oryza sativa] chromosome 4 clone OSJNBa0072F16, "* SEQUENC
G860 MSU91964 1.30E-35 Medicago sativa] MADS-box protein mRNA, partial eds.
G860 AP004766 3.60E-35 Oryza sativa japonica cultivar-group] chromosome 2 clone B10
G860 AW218280 9.00E-31 Lycopersicon esculentum] EST303461 tomato radicle, 5 d post-
G860 BM405213 2.90E-29 Solanum tuberosum] EST579540 potato roots Solanum tuberosum
G860 AW706936 3.10E-28 Glycine max] sk08d08.y1 Gm-c1023 Glycine max cDNA clone GENO
G860 AP004526 4.40E-28 Lotus japonicus] genomic DNA, chromosome 6, clone:LjT05E07,
Table 1
G860 gi1816459 8.20E-50 [Antirrhinum majus] DEFH125 protein.
G860 gi9964296 4.50E-46 [Zea mays] MADS box protein 2.
G860 gi1928874 1.00E-37 [Medicago sativa] MADS-box protein.
G860 gi2981133 2.20E-30 [Populus balsamifera subsp. trichocarpa] AGAMOUS homolog.
G860 gi 15081463 3.80E-29 [Ipomoea batatas] MADS-box protein.
G860 gi4887235 4.40E-29 [Hyacinthus orientalis] AGAMOUS homolog transcription facto
G860 gi13810204 6.00E-29 [Cucumis sativus] MADS1 protein.
G860 gi431736 2.50E-28 [Nicotiana tabacum] NAG1.
G860 gi13384058 9.60E-28 [Petunia x hybrida] MADS-box transcription factor FBP22.
G860 gi457382 2.10E-27 [Lycopersicon esculentum] TAG1.
G2347 BI931517 5.30E-31 [Lycopersicon esculentum] EST551406 tomato flower, 8 mm to pr
G2347 BE058432 4.20E-29 [Glycine max] sn16a06.y1 Gm-c1016 Glycine max cDNA clone GENO
G2347 AMSPB1 1.80E-28 [Antirrhinum majus] A.majus mRNA for squamosa-promoter bindin
G2347 BG525285 5.70E-28 Stevia rebaudiana] 48-3 Stevia field grown leaf cDNA Stevia
G2347 L38193 4.50E-27 [Brassica rapa] BNAF1025E Mustard flower buds Brassica rapa c
G2347 BG455868 6.30E-27 [Medicago truncatula] NF068F05PL1 F1045 Phosphate starved leaf
G2347 BG097153 1.70E-24 [Solanum tuberosum] EST461672 potato leaves and petioles Sola
G2347 BF482644 1.60E-23 Triticum aestivum] WHE2301-2304_A21_A21ZS Wheat pre-anthesis
G2347 AW747167 2.30E-23 [Sorghum bicolor] WS1_66_F11.b1_A002 Water-stressed 1 (WS1) S
G2347 BG442540 2.50E-23 [Gossypium arboreum] GA Ea0017G06f Gossypium arboreum 7-10 d
G2347 gi 1183864 1.50E-31 Antirrhinum majus] squamosa-promoter binding protein 2.
G2347 gi5931786 3.40E-25 Zea mays] SBP-domain protein 5.
G2347 gi8468036 1.40E-21 Oryza sativa] Similar to Arabidopsis thaliana chromosome 2
G2347 gi9087308 6.60E-09 Mitochondrion Beta vulgaris var altissima] orf102a.
G2347 gi7209500 0.83 Brassica rapa] S-locus pollen protein.
G722 BH477453 1.90E-61 Brassica oleracea] BOGXM90TR BOGX Brassica oleracea genomic
G722 AP004094 1.20E-50 Oryza sativa] chromosome 2 clone OJ1695_H09, *" SEQUENCING
G722 BM407041 1.40E-46 Solanum tuberosum] EST581368 potato roots Solanum tuberosum
G722 BM336723 1.20E-45 Zea mays] MEST198-A02.T3 ISUM5-RN Zea mays cDNA clone MEST19
G722 BE205320 2.60E-45 Medicago truncatula] EST397996 KVO Medicago truncatula cDNA
G722 AW564570 4.20E-40 Sorghum bicolor] LG1_296_E01.b1_A002 Light Grown 1 (LG1) Sor
Table 1
G722 BG523436 4.80E-36 [Stevia rebaudiana] 31-73 Stevia field grown leaf cDNA Stevia
G722 BJ181458 1.00E-33 [Physcomitrella patens subsp. patens] BJ181458 normalized ful
G722 AW596288 1.30E-33 [Glycine max] sj01e07.y1 Gm-c1032 Glycine max cDNA clone GENO
G722 BE214029 2.30E-31 [Hordeum vulgare] HV_CEb0001 P06f Hordeum vulgare seedling gre
G722 gi14189890 1.70E-52 [Zea mays] response regulator 9.
G722 gi15289981 2.20E-07 Oryza sativa] hypothetical protein.
G722 gi5916207 0.041 [Chlamydomonas reinhardtii] regulatory protein of P-starvat
G722 gi13173408 0.13 [Dianthus caryophyllus] response regulator protein.
G722 gi2224518 0.33 Chlorella vulgaris] ORF54d.
G722 gi7520715 0.33 [Chloroplast Chlorella vulgaris] NADH dehydrogenase (ubiqui
G722 gi4519671 0.99 Nicotiana tabacum] transfactor.
G1493 BH687265 6.20E-67 Brassica oleracea] BOMOL63TR BO_2_3_KB Brassica oleracea gen
G1493 AB060130 1.10E-65 [Zea mays] ZmRR8 mRNA for response regulator 8, complete eds.
G1493 AC107226 1.60E-63 [Oryza sativa] chromosome 3 clone OSJNBa0081 P02, "* SEQUENCI
G1493 AW719680 6.10E-50 [Lotus japonicus] LjNEST8C3r Lotus japonicus nodule library 5
G1493 BE660997 1.70E-47 Glycine max] 312 GmaxSC Glycine max cDNA, mRNA sequence.
G1493 BM326218 9.30E-42 [Sorghum bicolor] PIC1_72_C05.b1_A002 Pathogen-infected compa
G1493 BG450692 7.70E-39 [Medicago truncatula] NF102C07DT1 F1052 Drought Medicago trunc
G1493 BM407041 7.20E-38 [Solanum tuberosum] EST581368 potato roots Solanum tuberosum
G1493 BE353308 2.60E-31 [Lycopersicon hirsutum] EST400440 L. hirsutum trichome, Corne
G1493 BE214029 4.00E-30 Hordeum vulgare] HV_CEb0001P06f Hordeum vulgare seedling gre
G1493 gi15667625 4.00E-68 [Zea mays] response regulator 10.
G1493 gi13940498 2.50E-07 Oryza sativa] putative transcription factor OsGLKI .
G1493 gi 19322 0.0075 [Lycopersicon esculentum] glycine-rich protein.
G1493 gi6942190 0.074 [Mesembryanthemum crystallinum] CDPK substrate protein 1 ; C
G1493 gi5916207 0.13 Chlamydomonas reinhardtii] regulatory protein of P-starvat
G1493 gi2108256 0.18 [Bromheadia finlaysoniana] extensin.
G1493 gi4519671 0.22 Nicotiana tabacum] transfactor.
G1493 gi 18478480 0.72 Carica papaya] maturation-associated SRC1-like protein.
G1493 gi4456620 0.72 Hordeum vulgare] alpha-galactosidase.
G1493 gi3687688 0.87 Brassica napus] response regulator protein.
G475 BH659801 8.00E-34 Brassica oleracea] BOMFC96TF BO_2_3_KB Brassica oleracea gen
G475 AMSPB1 1.00E-33 Antirrhinum majus] A.majus mRNA for squamosa-promoter bindin
Table 1
25 G475 BI931517 3.30E-33 [Lycopersicon esculentum] EST551406 tomato flower, 8 mm to pr
25 G475 BG455868 8.20E-29 [Medicago truncatula] NF068F05PL1 F1045 Phosphate starved leaf
25 G475 BM732448 4.30E-28 [Glycine max] sal77d10.y1 Gm-c1061 Glycine max cDNA clone SOY
25 G475 BE922255 7.00E-28 [Solanum tuberosum] EST426024 potato leaves and petioles Sola
25 G475 BE499739 5.30E-25 fTriticum aestivum] WHE0975_G04_M07ZS Wheat pre-anthesis spik
25 G475 BI071295 1.40E-24 [Populus tremula x Populus tremuloides] C054P79U Populus stra
25 G475 BE060783 2.20E-23 [Hordeum vulgare] HVSMEg0013F15f Hordeum vulgare pre-anthesis
25 G475 AQ273505 1.60E-22 [Oryza sativa] nbxb0030O03f CUGI Rice BAC Library Oryza sativ
25 G475 gi 1183866 6.30E-36 [Antirrhinum majus] squamosa-promoter binding protein 1.
25 G475 gi5931786 8.60E-22 [Zea mays] SBP-domain protein 5.
25 G475 gi8468036 6.10E-13 [Oryza sativa] Similar to Arabidopsis thaliana chromosome 2
25 G475 gi9087308 2.20E-10 [Mitochondrion Beta vulgaris var. altissima] orf102a.
25 G475 gi6077100 0.15 [Brassica rapa] S-locus protein 1.
25 G475 gi6469127 0.21 [Cicer arietinum] famesylated protein.
25 G475 gi2224518 0.25 [Chlorella vulgaris] ORF54d.
C\ 25 G475 gi7520715 0.25 [Chloroplast Chlorella vulgaris] NADH dehydrogenase (ubiqui J
25 G475 gi1161252 0.28 [Glycine max] nucleosome assembly protein 1.
25 G475 gi 1778378 0.29 [Pisum sativum] NAPI Ps.
Table 2
SEQ ID Gene ID Test Sequence Test Sequence GenBank Annotation Reading High Smallest
NO ID Frame Score Sum Probability
1 G590 AW782148 AW782148 sm02b05.y1 Gm-c1027 Glycine max cDN... 1 115 8.60E-29 4
1 G590 AW832368 AW832368 sm08e11.y1 Gm-c1027 Glycine max cDN... 1 115 2.50E-19 3
1 G590 BH708336 BH708336 BOMKN39TF BO_2_3_KB Brassica olerac -2 238 6.10E-17 1
1 G590 AQ291034 AQ291034 nbxb0038K13r CUGI Rice BAC Library ... -3 157 1.30E-14 2
1 G590 BG352463 BG352463 sac05g04.y1 Gm-c1040 Glycine max cD... 3 110 2.40E-14 3
1 G590 AI484932 AI484932 EST243195 tomato ovary, TAMU Lycope... 3 111 1.40E-13 3
1 G590 AW649972 AW649972 EST328426 tomato germinating seedli... 3 111 2.10E-13 3
1 G590 AQ577669 AQ577669 nbxb0091 B05f CUGI Rice BAC Library ... -2 131 1.00E-12 2
1 G590 BE020147 BE020147 sm41g05.y1 Gm-c1028 Glycine max cDN... 1 115 1.00E-11 2
1 G590 AI489419 AI489419 EST247758 tomato ovary, TAMU Lycope... 3 104 2.30E-11 3
1 G590 AP004240 AP004240 Oryza sativa chromosome 2 clone OJ1... 3 154 2.20E-10 2
1 G590 AC069158 AC069158 Genomic Sequence for Oryza sativa, ... 2 154 2.90E-10 2
1 G590 AW650129 AW650129 EST328583 tomato germinating seedli... 2 113 5.50E-10 2
1 G590 AW761083 AW761083 sl70h09.y1 Gm-c1027 Glycine max cDN... 2 103 5.00E-09 2
1 G590 AP004010 AP004010 Oryza sativa chromosome 7 clone OJ1... 2 144 6.40E-09 2
1 G590 AP003824 AP003824 Oryza sativa chromosome 7 clone OJ1... 2 144 6.90E-09 2
1 G590 OSJN01000 AL607092 Oryza sativa chromosome 7 clone OSJ... -3 144 7.30E-09 2
1 G590 OSJN01003 AL607095 Oryza sativa chromosome 7 clone OSJ... -2 144 9.20E-09 2
1 G590 AW650578 AW650578 EST329032 tomato germinating seedli... 1 113 9.70E-09 2
1 G590 BG127545 BG127545 EST473287 tomato shoot/meristem Lye... 2 114 1.50E-08 2
1 G590 BI308330 BI308330 EST529740 GPOD Medicago truncatula ... 2 114 1.60E-08 2
1 G590 BI930358 BI930358 EST550247 tomato flower, 8 mm to pr... 3 113 2.60E-08 2
1 G590 BG124162 BG124162 EST469808 tomato shoot/meristem Lye... 3 113 3.00E-08 2
1 G590 BM408345 BM408345 EST582672 potato roots Solanum tube... 1 113 5.90E-08 2
1 G590 BF518953 BF518953 EST456346 DSIL Medicago truncatula ... 1 102 6.40E-08 2
1 G590 BH686591 BH686591 BOHZ031TR BO_2_3_KB Brassica olerac 2 106 1.10E-07 2
1 G590 BE555358 BE555358 sp89c07.y1 Gm-c1045 Glycine max cDN... 3 112 2.30E-07 2
1 G590 BI176631 BI176631 EST517576 cSTE Solanum tuberosum cD... 2 107 2.60E-07 2
1 G590 BH586940 BH586940 BOGKS90TR BOGK Brassica oleracea ge.. -2 110 4.60E-07 2
1 G590 BH535734 BH535734 BOGKB27TF BOGK Brassica oleracea ge... 2 106 7.40E-07 2
1 G590 AP002536 AP002536 Oryza sativa genomic DNA, chromosom... 2 116 8.60E-07 2
1 G590 AW704310 AW704310 sk17f07.y1 Gm-c1028 Glycine max cDN... 3 141 2.80E-06 1
1 G590 AW704341 AW704341 sk18b04.y1 Gm-c1028 Glycine max cDN... 3 141 2.80E-06 1
Table 2
1 G590 BG041496 BG041496 sv35a08.y1 Gm-c1057 Glycine max cDN... 2 99 4.30E-06 2
1 G590 AI898549 AI898549 EST267992 tomato ovary, TAMU Lycope... 2 104 5.10E-06 2
1 G590 BH011898 BH011898 TDGAA94TH cTOG Lycopersicon esculen... -2 134 2.30E-05 1
1 G590 BH012195 BH012195 TDGAG19TH cTOG Lycopersicon esculen... -2 134 2.30E-05 1
1 G590 BF052272 BF052272 EST437502 potato leaves and petiole... 3 91 3.40E-05 2
1 G590 BG591063 BG591063 EST498905 P. infestans-challenged I... 2 101 3.90E-05 2
1 G590 BM411362 BM411362 EST585689 tomato breaker fruit Lyco... 3 100 7.50E-05 2
1 G590 AP003020 AP003020 Oryza sativa genomic DNA, chromosom... -3 108 0.00012 2
1 G590 BG525606 BG525606 51-65 Stevia field grown leaf cDNA ... 2 89 0.00033 2
1 G590 BG526509 BG526509 59-79 Stevia field grown leaf cDNA ... 2 89 0.0008 2
1 G590 AC084320 AC084320 Oryza sativa chromosome 3 BAC OSJNB... 1 102 0.0057 2
G590 AC069300 AC069300 Oryza sativa chromosome 10 BAC OSJN... -1 98 0.016 2
G590 BI070102 BI070102 C014P11 U Populus strain T89 leaves ... 3 107 0.024
1 G590 BG052163 BG052163 RHIZ2_6_H10.b1_A003 Rhizome2 (RHIZ2... 1 104 0.025
1 G590 BG052162 BG052162 RHIZ2_6_H09.b1_A003 Rhizome2 (RHIZ2... 1 103 0.037
1 G590 BE211004 BE211004 so56c08.y1 Gm-c1039 Glycine max cDN... 1 104 0.041
1 G590 BH555770 BH555770 BOGP071TR BOGP Brassica oleracea ge... -1 117 0.057
1 G590 AW832651 AW832651 sm15f12.y1 Gm-c1027 Glycine max cDN... 2 100 0.069
1 G590 OSB6015 AL117264 Oryza sativa indica(GLA4) genomic D... 3 96 0.071 2
1 G590 BM323123 BM323123 PIC1_8_C08.b1_A002 Pathogen-infecte... 1 107 0.085
1 G590 OSJN00174 AL662970 Oryza sativa chromosome 4 clone OSJ... 2 96 0.086 2
1 G590 BF259788 BF259788 HVSMEf0020E07f Hordeum vulgare seed... 3 111 0.092
1 G590 BM377035 BM377035 EBem05_SQ003_O22_R IGF Barley EBemO... 1 95 0.23
1 G590 BG509554 BG509554 sad18e05.y1 Gm-c1074 Glycine max cD... 2 95 0.23
G590 BM443962 BM443962 EBem09_SQ002_F02_R IGF Barley EBemO... 1 96 0.24
G590 BF626292 BF626292 HVSMEa0018E07f Hordeum vulgare seed... 3 111 0.25
G590 BE431510 BE431510 EST336324 tomato breaker fruit, TIG... 2 108 0.27
G590 BH557513 BH557513 BOGJX59TF BOGJ Brassica oleracea ge... 1 110 0.3
G590 BE598711 BE598711 PI1_81_D03.b1_A002 Pathogen induced... 3 107 0.3
G590 BM377034 BM377034 EBem05_SQ003_O21_R IGF Barley EBemO... 1 92 0.43
G590 BE474728 BE474728 sp67h08.y1 Gm-c1044 Glycine max cDN... 3 103 0.47
G590 BH512680 BH512680 BOHMA32TF BOHM Brassica oleracea ge... -1 107 0.48
G590 AP004094 AP004094 Oryza sativa chromosome 2 clone OJ1... 2 87 0.5 2
G590 BE346647 BE346647 sp28d07.y1 Gm-c1042 Glycine max cDN... 1 102 0.51
G590 AP003975 AP003975 Oryza sativa chromosome 2 clone OJ1... 1 87 0.55 2
G590 AW647683 AW647683 EST307161 tomato germinating seedli... 3 104 0.61
Table 2
G590 C21906 C21906 C21906 Valencia orange young seeds cD... 1 92 0.62 G590 BI785116 BI785116 sai37b12.y1 Gm-c1065 Glycine max cD... 2 104 0.63 G590 BE353578 BE353578 EST353775 tomato flower buds 0-3 mm... 1 104 0.64 G590 BM325062 BM325062 PIC1_39_C08.b1_A002 Pathogen-infect... 2 101 0.71 G590 AV909206 AV909206 AV909206 K. Sato unpublished cDNA I... 2 104 0.72 G590 AV911091 AV911091 AV911091 K. Sato unpublished cDNA I... -2 104 0.77 G590 BE922593 BE922593 EST426362 potato leaves and petiole... 2 103 0.79 G590 BH454205 BH454205 BOGED20TR BOGE Brassica oleracea ge... 1 104 0.83 G590 BH013294 BH013294 TDGAW32TH cTOG Lycopersicon esculen... -1 100 0.86 G590 AU096311 AU096311 AU096311 Rice green shoot Oryza sat... 3 99 0.87
G590 BH550542 BH550542 BOGKR59TF BOGK Brassica oleracea ge... -3 92 0.93 G590 BG238234 BG238234 sab63h10.y1 Gm-c1043 Glycine max cD... 2 101 0.96 G590 AW775035 AW775035 EST334186 KV3 Medicago truncatula c. 1 101 0.96 G590 BM085798 BM085798 saj29f08.y1 Gm-c1066 Glycine max cD... 1 99 0.97 G590 BE604255 BE604255 WHE1413-1416J16J16ZS Wheat drough... -3 97 0.97 G590 BM445134 BM445134 EBem09_SQ007_M3_R IGF Barley EBemO... 1 96 0.97 G590 BM500529 BM500529 PAC000000000636 Pioneer AF-1 array ... 2 96 0.98 G590 BG517741 BG517741 947070G08.y1 947 - 2 week shoot fro... 3 95 0.9994 G590 BG367715 BG367715 HVSMEi0013F21f Hordeum vulgare 20 D... 2 96 0.9995 G590 BE659475 BE659475 GM700009B20A2 Gm-r1070 Glycine max ... -1 97 0.9996 G590 BG517733 BG517733 947070F09.y1 947 - 2 week shoot fro... 1 • 95 0.9998 G590 BI699666 BI699666 sai25f05.y1 Gm-c1053 Glycine max cD... 1 95 0.9998 G590 GI-15451582 AC069158_18 Putative SPATULA [Oryza sativa] -3 108 6.80E-16 4 G590 GI-14165335 AC069300_22 putative DNA-binding protein [... -3 102 1.40E-07 2
G590 GI-5852091 zwh14.1 -3 96 2.10E-06 2
G590 GI-13486760 hypothetical protein [Oryza sativa] -3 101 0.00043 3
G590 GI-13486646 hypothetical protein [Oryza sativa] -2 76 0.03 2
G590 GI-8468039 Similar to Arabidopsis thaliana chromosome... -1 73 0.045 3
G590 GI-12957703 AC084320_8 putative phytochrome interactin... -2 99 0.047 1
G590 GI-6166283 AF103808_1 helix-loop-helix protein 1A -2 73 0.18 2
G590 GI-7248461 root cap-specific protein [Zea mays] -1 64 0.88 1
G590 GI-2331141 water-stress inducible protein 3 63 0.94 1
G590 GI-5923912 AF185269_1 bHLH transcription factor GBOF-1 -2 66 0.95 2
G590 GI-4680497 AF119222_9 hypothetical protein 2 62 0.97 1 G590 GI-13346180 AF336279_1 GHDEL61 [Gossypium hirsutum] -2 67 0.97 2 G590 GI-21122 seed protein (AA 1 -83) -1 61 0.99 1
Table 2
G590 GI-81713 S04884 seed protein (clone p8B6) - radish -1 61 0.99
G590 GI-134420 SEEP_RAPSA LATE SEED MATURATION PROTEIN P8B6 -1 61 0.99
G590 GI-169692 late maturation protein -1 61 0.99
G590 GI-1086538 transcriptional activator Rb homolog -3 72 0.999
G590 GI-2130062 S72496 transcriptional activator Rb homolo... -3 72 0.999
G590 GI-527657 myc-like regulatory R gene product -3 74 0.999
G590 Gl,-3127045 bHLH transcription factor JAF13 -3 84 0.9996
G590 GI-5880765 AF137379_81 ChlB subunit of protochlorophy... -1 83 0.9997
G590 GI-5880838 AF137379_154 ChlB subunit of protochloroph... -1 83 0.9997
G590 GI-11467836 ChlB subunit of protochlorophyllide reduct... -1 83 0.9997
G590 GI-11467909 ChlB subunit of protochlorophyllide reduct... -1 83 0.9997
G590 GI-13878437 CHLB_NEPOL LIGHT-INDEPENDENT PROTOCHLOROPH. -1 83 0.9997
G590 GI-11862964 hypothetical protein [Oryza sativa] -2 66 0.9999 2
G590 GI-527655 myc-like regulatory R gene product -3 73 0.99991 1
3 G1760 AMDEFH125 Y10750 A.majus mRNA for DEFH125 protein. 8/... 1 264 8.40E-37 3
3 G1760 AW218280 AW218280 EST303461 tomato radicle, 5 d post... 3 253 4.40E-36 3
3 G1760 AI974336 AI974336 T110185e KVO Medicago truncatula c. 2 255 5.70E-36 3 os 3 G1760 OSJN00003 AL606460 Oryza sativa chromosome 4 clone OS... 1 268 5.70E-36 5
~4
3 G1760 AP004766 AP004766 Oryza sativa (japonica cultivar-gr... 1 259 2.20E-35 5
3 G1760 BG592879 BG592879 EST491557 cSTS Solanum tuberosum c... 3 256 2.20E-32 3
3 G1760 AL389596 AL389596 MtBC56A01 F1 MtBC Medicago truncatu... 2 222 6.10E-32 3
3 G1760 AX085162 AX085162 Sequence 12 from Patent WO0112798.... 1 260 6.80E-31 4
3 G1760 AX085359 AX085359 Sequence 12 from Patent WO0112799.... 1 260 6.80E-31 4
3 G1760 AX085151 AX085151 Sequence 1 from Patent WO0112798. ... 1 255 3.10E-30 3
3 G1760 AX085348 AX085348 Sequence 1 from Patent WO0112799. ... 1 255 3.10E-30 3
3 G1760 AF112149 AF112149 Zea mays MADS box protein 2 (mads2... 1 255 3.10E-30 3
3 G1760 BM405213 BM405213 EST579540 potato roots Solanum tub... 2 260 2.50E-28 2
3 G1760 AP003868 AP003868 Oryza sativa chromosome 8 clone OJ... 3 261 4.40E-28 4
3 G1760 AW706936 AW706936 sk08d08.y1 Gm-d 023 Glycine max cD... 2 256 2.70E-27 2
3 G1760 AP004526 AP004526 Lotus japonicus genomic DNA, chrom... -2 263 4.60E-27 3
3 G1760 BE596704 BE596704 PI1_58_F04.b1_A002 Pathogen induce... 1 260 7.10E-27 2
3 G1760 MSU91964 U91964 Medicago sativa MADS-box protein mRN... 1 228 4.20E-26 2
3 G1760 BE354988 BE354988 DG1_10_H09.b1_A002 Dark Grown 1 (D... 2 251 3.40E-25 2
3 G1760 AF198175 AF198175 Dendrobium grex Madame Thong-IN MA... 2 202 1.50E-20 2
3 G1760 BE193581 BE193581 HVSMEh0081 M10f Hordeum vulgare 5-4... 2 212 1.60E-20 2
3 G1760 PRU90349 U90349 Pinus radiata putative MADS box tran... 2 202 9.50E-20 2
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n n n co c n n c n o n n n n n o n n o n o n c n n n n n t n n n c n n n Table 2
G1760 BH523406 BH523406 BOGRZ47TF BOGR Brassica oleracea g... -3 229 1.30E-15 1
G1760 OSJN00086 AL606688 Oryza sativa chromosome 4 clone OS... 3 227 2.20E-15 2
G1760 BF638122 BF638122 NF044A02PL1 F1007 Phosphate starved... 2 227 2.90E-15
G1760 BF484557 BF484557 WHE2317_A03_A05ZS Wheat pre-anthes... 1 227 3.00E-15
G1760 BE493790 BE493790 WHE1275_E07_I13ZS Secale cereale a... 2 227 3.10E-15
G1760 AF305696 AF305696 Eucalyptus globulus MADS-box prote... 1 195 3.10E-15 2
G1760 BH744278 BH744278 gt35a01.b1 BoBudsOI Brassica olera... 1 227 3.30E-15
G1760 BG310152 BG310152 HVSMEc0016P20f Hordeum vulgare see... 2 225 3.70E-15
G1760 BE495206 BE495206 WHE1269_F04_L07ZS Secale cereale a... 1 227 3.70E-15
G1760 BE455888 BE455888 HVSMEg0017B20f Hordeum vulgare pre... 1 225 3.80E-15
G1760 AF306349 AF306349 Eucalyptus globulus MADS box prote... 1 195 4.00E-15 2
G1760 BJ195968 BJ195968 BJ195968 normalized full length cD... 2 226 4.80E-15
G1760 BE323629 BE323629 NF006D03PL1 F1026 Phosphate starved... 2 227 5.20E-15
G1760 BF483481 BF483481 WHE2334_D07_G14ZS Wheat pre-anthes... 3 225 5.40E-15
G1760 BE499751 BE499751 WHE0975_F02_K03ZS Wheat pre-anthes... 3 225 5.70E-15
G1760 BF291993 BF291993 WHE2203_D03_H05ZS Aegilops speltoi... 1 225 6.00E-15
G1760 BF479182 BF479182 L48-2837T3 Ice plant Lambda Uni-Za... 2 224 6.20E-15
G1760 BE498814 BE498814 WHE0966_E12_J24ZS Wheat pre-anthes... 3 225 6.70E-15
G1760 HVU249143 AJ249143 Hordeum vulgare mRNA for MADS-box ... 2 202 6.90E-15
G1760 BG274182 BG274182 WHE2230_E05_I10ZS Aegilops speltoi... 3 225 7.00E-15
G1760 BG274504 BG274504 WHE2237_G06_M11ZS Aegilops speltoi... 1 225 7.00E-15
G1760 AB050643 AB050643 Magnolia praecocissima mRNA for pu... 1 222 7.20E-15
G1760 BE637049 BE637049 WHE1821-1824_G05_G05ZS Secale cere... 1 225 8.50E-15
G1760 BF201770 BF201770 WHE1765_E01_J01ZS Wheat pre-anthes... 3 225 8.60E-15
G1760 ZMA292959 AJ292959 Zea mays mRNA for putative MADS-do... 1 232 9.80E-15
G1760 AI895411 AI895411 EST264854 tomato callus, TAMU Lyco... 1 223 1.00E-14
G1760 ZMA292961 AJ292961 Zea mays mRNA for putative MADS-do... 3 235 1.10E-14
G1760 RICOSMA L37526 Oryza sativa MADS-box protein (MADS2... 2 233 1.20E-14
G1760 BE430753 BE430753 SUN007.H09F991221 ITEC SUN Wheat c... 1 222 1.40E-14
G1760 AW331118 AW331118 707047D10.X1 707 - Mixed adult tis... 3 220 1.50E-14
G1760 AW738740 AW738740 EST340167 tomato flower buds, anth... 1 220 1.60E-14
G1760 AF072534 AF072534 Capsicum annuum pepper MADS-box pr... 1 219 1.70E-14
G1760 Bl176052 BI176052 EST517135 cSTS Solanum tuberosum c... 1 219 1.90E-14
G1 60 BG599624 BG599624 EST504519 cSTS Solanum tuberosum c... 1 219 1.90E-14
G1760 BF728292 BF728292 1000060C02.X1 1000 - Unigene I fro... 1 220 1.90E-14
G1760 BG789532 BG789532 sae65a02.y1 Gm-c1064 Glycine max c. 3 220 1.90E-14
Table 2
3 G1760 BI926036 BI926036 EST545925 tomato flower, buds 0-3 ... 1 221 1.90E-14
3 G1 60 BE344090 BE344090 EST409252 potato stolon, Cornell U... 2 220 2.00E-14
3 G1760 ZMA292960 AJ292960 Zea mays mRNA for putative MADS-do... 1 231 2.00E-14
3 G1760 BM405355 BM405355 EST579682 potato roots Solanum tub... 1 219 2.10E-14
3 G1760 AB026295 AB026295 Oryza sativa genomic DNA, chromoso... -3 217 2.20E-14
3 G1760 AW219022 AW219022 EST301504 tomato root during/after... 3 219 2.30E-14
3 G1760 LETDR3 X60756 L.esculentum TDR3 mRNA. 3/1992 3 219 2.30E-14
3 G1760 AP004516 AP004516 Lotus japonicus genomic DNA, chrom... 3 213 2.40E-14
3 G1760 AW039132 AW039132 EST281105 tomato mixed elicitor, B... 1 219 2.40E-14
3 G1760 AQ575051 AQ575051 nbxb0086L17r CUGI Rice BAC Library... 2 217 2.50E-14
3 G1760 BE249689 BE249689 NF020D04LF1 F1041 Developing leaf M... 3 222 2.60E-14
3 G1760 AC092556 AC092556 Oryza sativa chromosome 3 clone OS... 2 219 2.80E-14
3 G1760 BG596138 BG596138 EST494816 cSTS Solanum tuberosum c. 2 217 2.90E-14
3 G1760 BM111059 BM111059 EST558595 potato roots Solanum tub... 3 217 2.90E-14
3 G1760 AF377947 AF377947 Oryza sativa chromosome 3 clone OS... -3 219 2.90E-14
3 G1760 BI434165 BI434165 EST536926 P. infestans-challenged ... 1 217 2.90E-14
3 G1760 AC092697 AC092697 Oryza sativa chromosome 10 BAC OSJ... 1 209 3.00E-14
~4 3 G1760 AQ271415 AQ271415 nbxb0025B08f CUGI Rice BAC Library... 2 217 3.00E-14
° 3 G1760 BG591718 BG591718 EST499560 P. infestans-challenged ... 1 217 3.00E-14
3 G1760 AW289098 AW289098 707008H08.x3 707 - Mixed adult tis... 1 219 3.10E-14
3 G1760 BG 125538 BG125538 EST471184 tomato shoot/meristem Ly... 1 219 3.20E-14
3 G1760 BI263356 BI263356 NF089C12PL1 F1098 Phosphate starved... 1 217 3.30E-14
3 G1760 BG456966 BG456966 NF098D09PL1 F1076 Phosphate starved... 2 217 3.30E-14
3 G1760 AW010398 AW010398 ST06D02 Pine TriplEx shoot tip lib... 3 217 3.40E-14
3 G1760 BG889771 BG889771 EST515622 cSTD Solanum tuberosum c. 3 217 3.50E-14
3 G1760 BG525865 BG525865 53-44-1-2 r Stevia field grown lea... 1 216 3.70E-14
3 G1760 AQ330858 AQ330858 nbxb0048E10f CUGI Rice BAC Library... 2 217 3.70E-14
3 G1760 AW929235 AW929235 EST338023 tomato flower buds 8 mm ... 3 217 3.80E-14
3 G1760 AF275345 AF275345 Lycopersicon esculentum putative p... -2 238 4.10E-14
3 G1760 BG445047 BG445047 GA Ea0026l23f Gossypium arboreum ... 3 215 4.20E-14
3 G1760 BG343946 BG343946 HVSMEg0007D05f Hordeum vulgare pre... 3 215 4.30E-14
3 G1760 MD0251116 AJ251116 Malus domestica mRNA for B-type MA... 2 185 4.40E-14
3 G1760 AF150931 AF150931 Physcomitrella patens MADS-domain ... 2 233 4.60E-14
3 G1760 BE339799 BE339799 EST343859 potato stolon, Cornell U... 1 217 4.70E-14
3 G1760 PSA291298 AJ291298 Pisum sativum peam4 gene for MADS-... 2 201 4.80E-14
3 G1760 BE315880 BE315880 NF027E08LF1 F1055 Developing leaf M... 1 218 4.90E-14
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co co co co co co co co co co co co co co co co co co co co c co co co co co co co co co co co co co co co Table 2
3 G1760 BG442607 BG442607 GA Ea0017K04f Gossypium arboreum ... 3 209 2.70E-13 1
3 G1760 BE432051 BE432051 EST398580 tomato breaker fruit, TI... 3 210 2.70E-13 1
3 G1760 BG355470 BG355470 EM1_16_H03.b1_A002 Embryo 1 (EM1) ... 2 210 2.70E-13 1
3 G1760 BG933507 BG933507 WS1_4_C03.b1_A002 Water-stressed 1... 2 210 2.70E-13 1
3 G1760 AI728519 AI728519 BNLGHi10938 Six-day Cotton fiber G... 2 209 2.80E-13 1
3 G1760 GGN251557 AJ251557 Gnetum gnemon mRNA for putative MA... 2 226 2.80E-13 1
3 G1760 BE918053 BE918053 OV1_1_C11.b1_A002 Ovary 1 (OV1 ) So... 1 209 2.80E-13 1
3 G1760 AW924046 AW924046 WS1_49_D06.b1_A002 Water-stressed ... 2 210 2.80E-13 1
3 G1760 BG240312 BG240312 OV1_20_E03.b1_A002 Ovary 1 (OV1 ) S... 2 210 2.80E-13 1
3 G1760 BI933168 BI933168 EST553057 tomato flower, 8 mm to p... 3 208 2.80E-13 1
3 G1760 BF421169 BF421169 FM1_6_C07.b1_A003 Floral-Induced M... 2 210 2.80E-13 1
3 G1760 BF657564 BF657564 OV2_23_G05.b1_A002 Ovary 2 (OV2) S... 1 210 2.80E-13 1
3 G1760 AI898432 AI898432 EST267875 tomato ovary, TAMU Lycop... 1 208 2.80E-13 1
3 G1760 AF023615 AF023615 Pinus radiata MADS box protein mRN... 2 217 2.80E-13 1
3 G1760 AW678480 AW678480 WS1_16_D03.b1_A002 Water-stressed ... 3 210 2.80E-13 1
3 G1760 AL506480 AL506480 AL506480 Hordeum vulgare Barke dev... 1 208 2.90E-13 1
3 G1760 AL506894 AL506894 AL506894 Hordeum vulgare Barke dev... 2 208 2.90E-13 1
-4 3 G1760 AL507763 AL507763 AL507763 Hordeum vulgare Barke dev... 1 208 2.90E-13 1
" 3 G1760 AL508019 AL508019 AL508019 Hordeum vulgare Barke dev... 1 208 2.90E-13 1
3 G1760 AL509053 AL509053 AL509053 Hordeum vulgare Barke dev... 2 208 2.90E-13 1
3 G1760 AM61962 AI161962 A010P29U Hybrid aspen plasmid libr... 3 209 2.90E-13 1
3 G1760 BE918762 BE918762 OV1_4_F11.b1_A002 Ovary 1 (OV1 ) So... 1 209 2.90E-13 1
3 G1760 BE659971 BE659971 363 GmaxSC Glycine max cDNA, mRNA ... 2 210 2.90E-13 1
3 G1760 AH64140 AH 64140 A055P40U Hybrid aspen plasmid libr... 2 210 2.90E-13 1
3 G1760 BG357222 BG357222 OV2_11_C11.b1_A002 Ovary 2 (OV2) S... 3 209 2.90E-13 1
3 G1760 AI959798 AI959798 sc94d02.y1 Gm-c1019 Glycine max cD... 2 209 3.00E-13 1
3 G1760 AL506475 AL506475 AL506475 Hordeum vulgare Barke dev... 1 208 3.00E-13 1
3 G1760 AI487420 AI487420 EST245742 tomato ovary, TAMU Lycop... 1 208 3.00E-13 1
3 G1760 AF209729 AF209729 Hemerocallis hybrid cultivar putat... 1 207 3.10E-13 1
3 G1760 C73941 C73941 C73941 Rice panicle (longer than 10c... 1 209 3.10E-13 1
3 G1760 D89671 D89671 Ceratopteris richardii CerMADS2 mRNA... 1 226 3.20E-13 1
3 G1760 BM436799 BM436799 VVA010B05_53181 An expressed seque... 3 208 3.20E-13 1
3 G1760 AY036062 AY036062 Brassica napus SHATTERPROOF1 (BnSH... 1 207 3.20E-13 1
3 G1760 AL380342 AL380342 MtBB51 H02F1 MtBB Medicago truncatu... 1 209 3.30E-13 1
3 G1760 BE321182 BE321182 NF021 E06IN1 F1039 Insect herbivory ... 3 212 3.30E-13 1
3 G1760 BI434909 BI434909 EST537774 P. infestans-challenged ... 2 207 3.30E-13 1
Table 2
3 G1760 AW254974 AW254974 ML1145 peppermint glandular tricho... 2 209 3.30E-13
3 G1760 SAU25696 U25696 Sinapis alba transcription factor Sa... 1 213 3.30E-13
3 G1760 BM500137 BM500137 PAC000000000248 Pioneer AF-1 array... 1 208 3.30E-13
3 G1760 AW506862 AW506862 660060F01.y1 660 - Mixed stages of... 3 208 3.40E-13
3 G1760 AW442282 AW442282 EST311678 tomato fruit red ripe, T... 1 207 3.50E-13
3 G1760 BI543775 BI543775 FS6_F03 Sugar Beet inflorescence c... 2 207 3.50E-13
3 G1760 AF335237 AF335237 Petunia x hybrida MADS-box transcr... 1 215 3.60E-13
3 G1760 BF728277 BF728277 1000060A05.X1 1000 - Unigene I fro... 1 208 3.60E-13
3 G1760 AF060880 AF060880 Paulownia kawakamii MADS box prote... 1 223 3.60E-13
3 G1760 AL508733 AL508733 AL508733 Hordeum vulgare Barke dev... 2 207 3.70E-13
3 G1760 GGN132219 AJ132219 Gnetum gnemon mRNA for putative MA... 2 224 3.70E-13
3 G1760 BE434089 BE434089 EST405167 tomato breaker fruit, TI... 3 208 3.70E-13
3 G1760 AI771 96 AI771796 EST252896 tomato ovary, TAMU Lycop... 3 208 3.70E-13
3 G1760 AI486290 AI486290 EST244611 tomato ovary, TAMU Lycop... 3 208 3.70E-13
3 G1760 BE433121 BE433121 EST399650 tomato breaker fruit, TI... 3 208 3.70E-13
3 G1760 AI483740 AI483740 EST249611 tomato ovary, TAMU Lycop... 2 208 3.80E-13
3 G1760 AF335238 AF335238 Petunia x hybrida MADS-box transcr... 1 206 3.90E-13
-4 3 G1760 BM500293 BM500293 PAC000000001427 Pioneer AF-1 array... 1 208 3.90E-13
* 3 G1760 C73896 C73896 C73896 Rice panicle (longer than 10c... 3 209 3.90E-13
3 G1760 AU029711 AU029711 AU029711 Rice panicle shorter than... 3 209 3.90E-13
3 G1760 BG367358 BG367358 HVSMEi0011 P20f Hordeum vulgare 20 ... 2 208 3.90E-13
3 G1760 AW442341 AW442341 EST311737 tomato fruit red ripe, T... 3 207 4.00E-13
3 G1760 AI161447 AH 61447 A001 P09U Hybrid aspen plasmid libr... 3 210 4.10E-13
3 G1760 AW 129875 AW 129875 707002A11.y1 707 - Mixed adult tis... 3 209 4.10E-13
3 G1760 AF315464 AF315464 Petunia x hybrida cv. Dazzler MADS... 1 223 4.20E-13
3 G1760 BG054385 BG054385 OV2_3_G06.b1_A002 Ovary 2 (OV2) So... 1 209 4.20E-13
3 G1760 AI486089 AI486089 EST244410 tomato ovary, TAMU Lycop... 3 207 4.20E-13
3 G1760 BM406322 BM406322 EST580649 potato roots Solanum tub... 3 207 4.20E-13
3 G1760 AW929667 AW929667 EST338455 tomato flower buds 8 mm ... 3 208 4.20E-13
3 G1760 BE435698 BE435698 EST406776 tomato breaker fruit, TI... 1 207 4.30E-13
3 G1760 BM413110 BM413110 EST587437 tomato breaker fruit Lye... 2 207 4.30E-13
3 G1760 AI487178 AI487178 EST245500 tomato ovary, TAMU Lycop... 2 208 4.30E-13
3 G1760 AI490605 AI490605 EST249159 tomato ovary, TAMU Lycop... 1 208 4.30E-13
3 G1760 AI897069 AI897069 EST266512 tomato ovary, TAMU Lycop... 2 208 4.30E-13
3 G1760 BM535997 BM535997 EST589019 tomato breaker fruit Lye... 2 207 4.40E-13
3 G1760 BG446848 BG446848 GA Eb0039l21f Gossypium arboreum ... 2 207 4.40E-13
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co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co Table 2
3 G1760 GHY9726 AJ009726 Gerbera hybrida mRNA for MADS-box ... 2 204 6.60E-13 1
3 G1760 BI935126 BI935126 EST555015 tomato flower, anthesis ... 3 204 6.70E-13 1
3 G1760 BM535524 BM535524 EST588557 tomato breaker fruit Lye... 2 207 6.70E-13 1
3 G1760 BF112397 BF112397 EST439987 tomato breaker fruit Lye... 3 208 6.90E-13 1
3 G1760 BG262504 BG262504 WHE0936_G06_N12ZS Wheat 5-15 DAP s... 1 206 6.90E-13 1
3 G1760 AF120097 AF120097 Pinus radiata DEF/GLO-like protein... 1 220 7.20E-13 1
3 G1760 BM137344 BM137344 WHE2632_F08_L16ZS Wheat Fusarium g... 1 205 7.30E-13 1
3 G1760 BM412354 BM412354 EST586681 tomato breaker fruit Lye... 3 204 7.30E-13 1
3 G1760 BM412293 BM412293 EST586620 tomato breaker fruit Lye... 1 204 7.40E-13 1
3 G1760 BG444639 BG444639 GA Ea0025A08f Gossypium arboreum ... 2 212 7.50E-13 1
3 G1760 BE805943 BE805943 ss51f05.y1 Gm-c1061 Glycine max cD... 3 206 7.60E-13 1
3 G1760 AW928593 AW928593 EST337381 tomato flower buds 8 mm ... 2 208 7.70E-13 1
3 G1760 AI486645 AI486645 EST244966 tomato ovary, TAMU Lycop... 2 204 7.80E-13 1
3 G1760 AF335242 AF335242 Petunia x hybrida MADS-box transcr... 1 217 7.90E-13 1
3 G1760 BI928748 BI928748 EST548637 tomato flower, 3 - 8 mm ... 1 204 7.90E-13 1
3 G1760 AW981881 AW981881 PC19G08 Pine TriplEx pollen cone I... 1 205 7.90E-13 1
3 G1760 BI933057 BI933057 EST552946 tomato flower, 8 mm to p... 3 204 8.10E-13 1 3 G1760 AW930971 AW930971 EST356814 tomato fruit mature gree... 1 204 8.20E-13 1 3 G1760 AI771416 AI771416 EST252516 tomato ovary, TAMU Lycop... 1 208 8.20E-13 1
3 G1760 MDAJ762 AJ000762 Malus domestica mRNA for MADS-box ... 3 210 8.40E-13 1
3 G1760 AI486208 AI486208 EST244529 tomato ovary, TAMU Lycop... 2 204 8.40E-13 1
3 G1760 BI930479 BI930479 EST550368 tomato flower, 8 mm to p... 3 204 8.40E-13 1
3 G1760 BM411808 BM411808 EST586124 tomato breaker fruit Lye... 1 204 8.40E-13 1
3 G1760 AW559625 AW559625 EST314673 DSIR Medicago truncatula... 2 204 8.40E-13 1
3 G1760 AW441346 AW441346 EST310742 tomato fruit red ripe, T... 1 204 8.40E-13 1
3 G1760 AI484993 AI484993 EST243256 tomato ovary, TAMU Lycop... 2 204 8.60E-13 1
3 G1760 BE445107 BE445107 WHE1132_G12_N24ZS Wheat etiolated ... 3 205 8.60E-13 1
3 G1760 AW309024 AW309024 sf93h07.y1 Gm-c1019 Glycine max cD... 3 204 8.60E-13 1
3 G1760 AW737591 AW737591 EST339018 tomato flower buds, anth... 1 204 8.80E-13 1
3 G1760 AW934007 AW934007 EST359850 tomato fruit mature gree... 1 204 8.80E-13 1
3 G1760 AW934390 AW934390 EST360233 tomato fruit mature gree... 1 204 8.80E-13 1
3 G1760 BE034098 BE034098 MG05B05 MG Mesembryanthemum crysta... 1 203 8.80E-13 1
3 G1760 AW441624 AW441624 EST311020 tomato fruit red ripe, T... 3 204 8.80E-13 1
3 G1760 AI485336 AI485336 EST243657 tomato ovary, TAMU Lycop... 1 204 8.90E-13 1
3 G1760 BE231150 BE231150 HVSMEg0017C04f Hordeum vulgare pre... 2 215 9.00E-13 1
3 G1760 BG274189 BG274189 WHE2230_F01_K02ZS Aegilops speltoi... 2 205 9.10E-13 1
LoU
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Figure imgf000080_0001
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S 5 5 S LU S LU LL LU LL S 2 lU 5 2 2 S -3 θ LL LL Dj LU !t LL Q] LL LL !t lL llJ S 5 y 2 -3 < < < < CQ < CO CO CQ CQ < < CO < < < < < CO CQ CO < CQ < CQ < < CQ < CD CQ < N Q- CO <
0 0 0 0 0 0 O 0 0 0 0 O O 0 O 0 0 0 O O 0 0 0 0 0 0 0 O 0 O 0 0 0 O O 0 CO CO CO CO CO CO CO CO CD CO CO CO CO CO CO CD CO CO CD CD CO CO CD CD CO CO CO CO CD CD CD CO CO CD CO CO i — r— t-— i — i — r — r-~ r-- r — i — r — ι-— — r — i — ι-— r — r — i — i — r — i — i--- r — i — r — r — r — r — r — r-— r — t — i — i — r-~ δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ cr) co co co c co co co co co co co co co co co co co co co co co n co co co co co co co co co co co co co Table 2
3 G1760 BG626444 BG626444 cC-esflcLEL12J22d1 Tomato flower I... 2 204 1.20E-12 1
3 G1760 BE921657 BE921657 EST425426 potato leaves and petiol... 2 203 1.20E-12 1
3 G1760 NTTOB X76188 N.tabacum tobmadsl mRNA. 9/1994 2 218 1.20E-12 1
3 G1760 AF198176 AF198176 Dendrobium grex Madame Thong-IN MA... 3 202 1.30E-12 1
3 G1760 B 536192 BM536192 EST589214 tomato breaker fruit Lye... 2 207 1.30E-12 1
3 G1760 GGN132209 AJ132209 Gnetum gnemon mRNA for putative MA... 1 218 1.30E-12 1
3 G1760 AI897334 AI897334 EST266693 tomato ovary, TAMU Lycop... 3 204 1.40E-12 1
3 G1760 AI900863 AI900863 sb95d06.y1 Gm-c1012 Glycine max cD... 1 203 1.40E-12 1
3 G1760 AF029975 AF029975 Eucalyptus grandis MADS box protei... 1 216 1.40E-12 1
3 G1760 BG445408 BG445408 GA Ea0028G20f Gossypium arboreum ... 3 210 1.40E-12 1
3 G1760 AI486250 AI486250 EST244571 tomato ovary, TAMU Lycop... 2 204 1.40E-12 1
3 G1760 AI899370 AI899370 EST268813 tomato ovary, TAMU Lycop... 2 204 1.40E-12 1
3 G1760 BE500707 BE500707 WHE0991-0994J21J21ZS Wheat pre-a... 3 203 1.40E-12 1
3 G1760 BF324502 BF324502 su12g02.y1 Gm-c1066 Glycine max cD... 2 202 1.40E-12 1
3 G1760 BG241308 BG241308 OV1_40_G05.b1_A002 Ovary 1 (OV1 ) S... 2 203 1.40E-12 1
3 G1760 AI490968 AI490968 EST241677 tomato shoot, Cornell Ly... 3 202 1.40E-12 1
3 G1760 BG240086 BG240086 OV1_17_G05.b1_A002 Ovary 1 (OV1 ) S... 2 203 1.40E-12 1 oe 3 G1760 AW266670 AW266670 L0-1492T3 Ice plant Lambda Uni-Zap... 1 203 1.50E-12 1
° 3 G1760 AF035378 AF035378 Lolium temulentum MADS-box protein... 2 218 1.50E-12 1
3 G1760 AF144623 AF144623 Canavalia lineata MADS-box transcr... 3 215 1.50E-12 1
3 G1760 BG596731 BG596731 EST495409 cSTS Solanum tuberosum c... 2 201 1.60E-12 1
3 G1760 PRU76726 U76726 Pinus radiata MADS-box protein (PrMA... 1 217 1.60E-12 1
3 G1760 BG415259 BG415259 HVSMEk0005M04f Hordeum vulgare tes... 2 210 1.60E-12 1
3 G1760 AX099212 AX099212 Sequence 1 from Patent WO0119995. ... 2 202 1.70E-12 1
3 G1760 BI405804 BI405804 146B05 Mature tuber lambda ZAP Sol... 1 201 1.70E-12 1
3 G1760 B1957577 BI957577 HVSMEn0010D09f Hordeum vulgare rac... 2 201 1.70E-12 1
3 G1760 BE416369 BE416369 MUG008.A01 R990628 ITEC MUG Wheat S... 2 202 1.80E-12 1
3 G1760 D46933 D46933 RICS11905A Rice green shoot Oryza sa... 1 203 1.80E-12 1
3 G1760 BE055946 BE055946 945020E05.Y1 945 - Mixed adult tis... 1 202 1.80E-12 1
3 G1760 BG418810 BG418810 HVSMEk0024l17f Hordeum vulgare tes... 2 207 1.80E-12 1
3 G1760 BF705575 BF705575 RHIZ2_4_H03.b1_A003 Rhizome2 (RHIZ... 2 203 1.80E-12 1
3 G1760 AB022665 AB022665 Gnetum parvifolium GpMADS3 mRNA, c... 2 217 1.80E-12 1
3 G1760 AI484747 AI484747 EST243008 tomato ovary, TAMU Lycop... 3 201 1.80E-12 1
3 G1760 PRU90344 U90344 Pinus radiata putative MADS box tran... 2 215 1.80E-12 1
3 G1760 BE416238 BE416238 MUG006.B01 R990218 ITEC MUG Wheat S... 2 202 1.80E-12 1
3 G1760 AW278878 AW278878 sf99h09.y1 Gm-c1019 Glycine max cD... 3 201 1.80E-12 1
Table 2
G1760 BF627446 BF627446 HVSMEb0004O03f Hordeum vulgare see.. 200 1.90E-12
G1760 AI483806 AI483806 EST249677 tomato ovary, TAMU Lycop... 202 1.90E-12
G1760 BE451798 BE451798 NXCI_002_D03_F NXCI (Nsf Xylem Com.. 202 2.00E-12
G1760 AX074057 AX074057 Sequence 8 from Patent WO0104315. ... 222 2.00E-12
G1760 BF050801 BF050801 EST435959 tomato developing/immatu... 201 2.00E-12
G1760 GGN132215 AJ132215 Gnetum gnemon mRNA for putative MA... 217 2.00E-12
G1760 BG367455 BG367455 HVSMEi0012F04f Hordeum vulgare 20 ... 200 2.00E-12
G1760 BE474886 BE474886 sp69f12.y1 Gm-c1044 Glycine max cD... 202 2.00E-12
G1760 BE186233 BE186233 945038A02.X2 945 - Mixed adult tis... 201 2.00E-12
G1760 AF099937 AF099937 Hyacinthus orientalis AGAMOUS homo... 215 2.10E-12
G1760 GI-1928874 MADS-box protein 228 4.50E-48 6
G1760 GI-7488744 T09700 MADS-box protein - alfalfa (fragment) 228 4.50E-48 6
G1760 GI-1816459 DEFH 125 protein 264 3.30E-47 5
G1760 GI-7489303 T17029 MADS-box transcription factor DEFH1... 264 3.30E-47 5
G1760 GI-9964296 AF112149_1 MADS box protein 2 [Zea mays] 255 2.50E-44 4
G1760 GI-15081463 AF396746_1 MADS-box protein [Ipomoea batatas] 219 8.40E-37 5
G1760 GI-1568513 fbpn 208 1.70E-27 4
G1760 GI-1914838 MADS box protein 207 3.50E-27 4
G1760 GI-5295964 EST D15657(C1032) corresponds to a region... 207 3.50E-27 4
G1760 GI-7446542 T04168 MADS box protein - rice 207 3.50E-27 4
G1760 GI-1067169 floral binding protein number 7 201 5.90E-27 4
G1760 GI-861081 agamous 209 1.20E-26 3
G1760 GI-3913005 AG_PANGI AGAMOUS PROTEIN (GAG2) 209 1.20E-26 3
G1760 GI-16973298 C-type MADS box protein [Malus x domestica] 209 3.00E-26 3
G1760 GI-6840998 AF120097_1 DEF/GLO-like protein 220 4.30E-26 4
G1760 GI-602900 SLM1 211 4.80E-26 3
G1760 GI-4103342 agamous-like putative transcription factor 212 6.10E-26 3
G1760 GI-2981131 AGAMOUS homolog 207 6.30E-26 3
G1760 GI-2981133 AGAMOUS homolog 208 8.00E-26 3
G1760 GI-4102113 NAP 1-2 199 2.00E-25 4
G1760 GI-2997613 CUM1 215 2.50E-25 3
G1760 GI-4103346 agamous-like putative transcription factor 215 2.50E-25 3
G1760 GI-7446520 T08039 MADS-box protein - cucumber 215 2.50E-25 3
G1760 GI-9994504 FBP15 [Petunia x hybrida] 215 2.50E-25 3
G1760 GI-4103757 MADS1 211 2.60E-25 3
G1760 GI-2252482 putative MADS domain transcription factor 216 3.60E-25 4
Table 2
G1760 GI-2997615 CUM10 212 4.10E-25 3
G1760 GI-7446521 T08040 MADS-box protein - cucumber 212 4.10E-25 3
G1760 GI-4218160 MADS-box protein, GAGA1 212 4.10E-25 3
G1760 GI-4218162 MADS-box protein, GAGA2 212 4.10E-25 3
G1760 GI-4204232 MADS-box protein 1 218 5.00E-25 3
G1760 GI-13177638 AF265554_1 transcription factor CMB [Cucum... 212 5.20E-25 3
G1760 GI-8745072 MADS box protein [Betula pendula] 211 5.20E-25 3
G1760 GI-5070144 AF068726_1 MADS-box protein MADS2 198 5.30E-25 4
G1760 GI-5070140 AF068724_1 MADS-box protein MADS5 195 5.40E-25 4
G1760 GI-167126 BAG1 207 6.80E-25 3
G1760 GI-322650 A43484 probable transcription factor BAG1 ... 207 6.80E-25 3
G1760 GI-399096 AG_BRANA AGAMOUS PROTEIN 207 6.80E-25 3
G1760 GI-9956938 AF150931_1 MADS-domain protein PPM1 [Physc... 233 7.40E-25 3
G1760 GI-9956940 AF150932_1 MADS-domain protein PPM1 [Physc... 233 7.40E-25 3
G1760 GI-4101710 MADS box transcription factor 217 8.10E-25 3
G1760 GI-1321797 putative transcription factor 210 8.50E-25 3
G1760 GI-4103344 agamous-like putative transcription factor 210 8.50E-25 3
G1760 GI-7446562 T10185 MADS-box protein CUS1 - cucumber 210 8.50E-25 3
G1760 GI-695688 DAL2 protein 217 1.00E-24 3
G1760 GI-4033710 AGAMOUS-like MADS-box transcription factor... 217 1.OOE-24 3
G1760 GI-4033721 AGAMOUS-like MADS-box transcriptional fact... 217 1.00E-24 3
G1760 GI-4033723 AGAMOUS-like MADS-box transcriptional fact... 217 1.OOE-24 3
G1760 GI-4103486 MADS box protein 217 1.OOE-24 3
G1760 GI-7446536 T14847 MADS-box protein dal2 - Norway spruce 217 1.OOE-24 3
G1760 GI-5019460 putative MADS domain transcription factor ... 213 1.10E-24 3
G1760 GI-16973296 C-type MADS box protein [Malus x domestica] 209 1.10E-24 3
G1760 GI-6970411 MADS-box protein 209 1.40E-24 3
G1760 GI-3646340 MADS-box protein 223 1.60E-24 3
G1760 GI-3688589 MADS box transcription factor 215 1.70E-24 3
G1760 GI-4887235 AGAMOUS homolog transcription factor 215 1.70E-24 3
G1760 GI-939779 MADS box protein 213 1.70E-24 3
G1760 GI-7446514 T03398 MADS box protein - maize 213 1.70E-24 3
G1760 GI-9956942 AF150933_1 MADS-domain protein PPM2 [Physc... 233 1.90E-24 3
G1760 GI-9956944 AF150934_1 MADS-domain protein PPM2 [Physc... 233 1.90E-24 3
G1760 GI-5019464 putative MADS domain transcription factor ... 224 2.00E-24 3
G1760 GI-886405 MADS box protein 215 2.10E-24 3
Table 2
G1760 GI-2130078 S59480 MADS-box protein 3 - rice 1 215 2.10E-24 3
G1760 GI-13161415 putative MADS-box protein [Oryza sativa] 1 215 2.10E-24 3
G1760 GI-695686 daM 1 217 2.70E-24 3
G1760 GI-2160701 MADS-box protein 1 217 2.70E-24 3
G1760 GI-7446537 T14846 probable MADS-box protein dall - No... 1 217 2.70E-24 3
G1760 GI-7446551 T09603 MADS-box protein 3 - Monterey pine 1 217 2.70E-24 3
G1760 GI-14279306 AF265562_1 MAD-box transcripion factor [Vi... 1 215 2.70E-24 3
G1760 GI-13958339 AF372840_1 madsl [Poa annua] 1 213 2.70E-24 3
G1760 GI-6006607 MADS box protein, MADS28 1 202 2.90E-24 3
G1760 G1-6092011 GpMADS4 1 220 3.30E-24 3
G1760 GI-1206005 putative MADS-box family transcription factor 1 213 3.50E-24 3
G1760 GI-1702951 MADS-box family transcription factor 1 213 3.50E-24 3
G1760 GI-7446546 T10486 MADS box protein - Canadian red pine 1 213 3.50E-24 3
G1760 GI-7446559 T09571 MADS box protein MADS2 - Monterey pine 1 213 3.50E-24 3
G1760 GI-9367307 MADS-box protein 3 [Hordeum vulgare] 1 202 3.70E-24 3
G1760 GI-5031217 AGAMOUS homolog 1 214 4.40E-24 3
G1760 GI-3688591 MADS box transcription factor 1 213 4.40E-24 3
G1760 GI-6175371 AF091458_1 MADS box transcription factor M... 1 202 5.90E-24 3
G1760 GI-5019431 putative MADS domain transcription factor ... 1 218 6.80E-24 3
G1760 GI-2286109 MADS box protein 1 213 7.10E-24 3
G1760 GI-7446517 T04167 MADS box protein - rice 1 213 7.10E-24 3
G1760 GI-13810204 MADS1 protein [Cucumis sativus] 1 212 7.10E-24 3
G1760 GI-939781 MADS box protein 1 209 7.20E-24 3
G1760 GI-7446515 T03408 MADS box protein - maize 1 209 7.20E-24 3
G1760 GI-9367313 MADS-box protein 8 [Hordeum vulgare] 1 203 7.50E-24 3
G1760 GI-602908 SLM5 1 203 1.20E-23 3
G1760 GI-16052 SQUA 1 201 1.20E-23 3
G1760 GI-82313 S20886 transcription factor squa - garden ... 1 201 1.20E-23 3
G1760 GI-18076209 putative MADS-domain transcription factor ... 1 225 1.30E-23 3
G1760 GI-7592642 API -like MADS box protein [Oryza sativa] 1 210 1.50E-23 3
G1760 GI-602906 SLM4 1 204 1.50E-23 3
G1760 GI-7544096 pMADS4 [Petunia x hybrida] 1 210 1.90E-23 3
G1760 GI-6634708 AF176782_1 MADS box transcription factor 1 206 1.90E-23 3
G1760 GI-6731756 unnamed protein product 1 206 1.90E-23 3
G1760 GI-12019658 FDRMADS6 [Oryza sativa] 1 208 2.40E-23 3
G1760 GI-4204234 MADS-box protein 2 1 202 2.50E-23 3
Table 2
G1760 GI-3170502 APETALA3 homolog PnAP3-2 1 214 3.60E-23 2
G1760 GI-848999 agamous protein 1 210 3.80E-23 3
G1760 GI-13384068 AF335245_1 MADS-box transcription factor F... 1 199 4.00E-23 3
G1760 GI-5295986 MADS box-like protein 1 198 4.50E-23 4
G1760 GI-887579 MADS box regulatory protein 1 211 4.80E-23 3
G1760 GI-1046276 MADS box regulatory protein 1 211 4.80E-23 3
G1760 GI-1364102 S57586 MADS box regulatory protein - Rumex... 1 211 4.80E-23 3
G1760 GI-5019429 putative MADS domain transcription factor ... 1 220 5.60E-23 2
G1760 GI-1483230 MADS4 protein 1 206 6.20E-23 3
G1760 GI-264223 promotes sex organ development=ple [Antirr... 1 206 6.20E-23 3
G1760 GI-322801 A44343 promotes sex organ development prot... 1 206 6.20E-23 3
G1760 GI-5019456 putative MADS domain transcription factor ... 1 217 7.20E-23 2
G1760 GI-6092009 GpMADS3 1 217 7.20E-23 2
G1760 GI-508577 box protein 1 208 7.50E-23 2
G1760 GI-1076739 S53306 box protein - rice 1 208 7.50E-23 2
G1760 GI-10188329 unnamed protein product [Zea mays] 1 208 7.50E-23 2
G1760 GI-11493807 MADS box protein MADS1 [Oryza sativa] 1 208 7.50E-23 2
G1760 GI-6468290 putative MADS domain transcription factor ... 1 209 7.70E-23 3
G1760 GI-19382 TDR4 1 207 7.80E-23 3
G1760 GI-542035 S23730 TDR4 protein - tomato 1 207 7.80E-23 3
G1760 GI-3912999 AGL8J.YCES FLORAL HOMEOTIC PROTEIN AGL8 HO. 1 207 7.80E-23 3
G1760 GI-396199 fbp6 1 210 9.80E-23 3
G1760 GI-2129972 S60307 fbp6 protein - garden petunia 1 210 9.80E-23 3
G1760 GI-6606070 AF058697_1 MADS14 protein 1 202 1.00E-22 3
G1760 GI-5051937 MADS-box protein FDRMADS2 1 164 1.20E-22 4
G1760 GI-1239959 MADS-box gene 1 210 1.20E-22 3
G1760 GI-7446511 S71757 MADS box protein DEFH200 - garden s... 1 210 1.20E-22 3
G1760 GI-6467974 AF198175_1 MADS box protein DOMADS2 1 202 1.20E-22 2
G1760 GI-6469345 DNA-binding protein 1 198 1.30E-22 3
G1760 GI-642591 amino acid feature: K-box, bp 283..480; am... 1 198 1.30E-22 3
G1760 GI-1561784 homeotic protein boiCAL 1 198 1.30E-22 3
G1760 GI-7446539 T14456 MADS box protein homolog CAL - wild... 1 198 1.30E-22 3
G1760 GI-9964074 MADS-box transcription factor jointless [L... 1 223 1.50E-22 2
G1760 GI-17433048 JOIN_LYCES MADS-box JOINTLESS protein (LeM... 1 223 1.50E-22 2
G1760 GI-18996775 AF462152_1 MADS-box transcription factor H... 1 202 1.60E-22 3
G1760 GI-9367309 MADS-box protein 5 [Hordeum vulgare] 1 196 1.70E-22 3
Table 2
G1760 GI-439239 MADS-box protein 1 201 2.10E-22 3
G1760 GI-632112 S40405 MADS-box protein - Aranda deborah 1 201 2.10E-22 3
G1760 GI-3912996 AGL9_ARADE FLORAL HOMEOTIC PROTEIN AGL9 HO... 1 201 2.10E-22 3
G1760 GI-4033725 AGAMOUS-like MADS-box transcriptional fact... 1 195 2.20E-22 3
G1760 GI-10880315 putative MADS-domain transcription factor ... 1 226 2.30E-22 2
G1760 GI-13384062 AF335242_1 MADS-box transcription factor F... 1 217 2.40E-22 3
G1760 GI-5295980 MADS box-like protein 1 209 2.50E-22 2
G1760 GI-1239963 MADS-box gene 1 207 2.60E-22 3
G1760 GI-7446510 S71756 MADS box protein DEFH72 - garden sn... 1 207 2.60E-22 3
G1760 GI-758565 transcription factor 1 207 2.60E-22 3
G1760 GI-758567 transcription factor 1 207 2.60E-22 3
G1760 GI-3913001 AGL8_SOLTU FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 207 2.60E-22 3
G1760 GI-7446508 T07100 MADS box protein homolog POTM1-1 - ... 1 207 2.60E-22 3
G1760 GI-3646320 MADS-box protein 1 197 2.70E-22 3
G1760 GI-16549058 putative MADS-domain transcription factor ... 1 222 3.00E-22 2
G1760 GI-6650550 AF109153_1 MADS box transcription factor M... 1 213 3.10E-22 2
G1760 GI-457382 TAG1 1 208 3.20E-22 2
G1760 GI-3913004 AG_LYCES AGAMOUS PROTEIN (TAG1) 1 208 3.20E-22 2
G1760 GI-7446522 T07185 floral homeotic protein TAG1 - tomato 1 208 3.20E-22 2
G1760 GI-10946429 AF305076_1 MADS-box protein EAP1 [Eucalypt... 1 195 3.50E-22 3
G1760 GI-16874557 MADS-box transcription factor DEFH28 [Anti... 1 201 4.30E-22 3
G1760 GI-887392 BOAP1 1 192 4.50E-22 3
G1760 GI-431736 NAG1 1 212 5.10E-22 2
G1760 GI-3913007 AG_TOBAC AGAMOUS PROTEIN (NAG1) 1 212 5.10E-22 2
G1760 GI-7446523 T03592 floral homeotic protein NAG1 - comm... 1 212 5.10E-22 2
G1760 GI-3688593 MADS box transcription factor 1 211 5.10E-22 2
G1760 GI-16417764 AF424549_1 MADS-box protein SPW1 [Oryza sa... 1 206 5.20E-22 2
G1760 GI-15077026 AF286649_1 transcription factor CMB1 [Cucu... 1 186 5.90E-22 3
G1760 GI-13448658 MADS box transcription factor [Ipomoea bat... 1 220 6.20E-22 2
G1760 GI-3646324 MADS-box protein 1 213 6.40E-22 2
G1760 GI-5777904 U78949_1 MADS-box protein 3 1 213 6.40E-22 2
G1760 GI-4322475 putative MADS box transcription factor ETL 1 209 6.50E-22 2
G1760 GI-4406132 MADS box protein 1 206 6.60E-22 2
G1760 GI-15824795 AF345911_1 MADS-box protein FDRMADS3 [Oryz... 1 205 6.60E-22 2
G1 60 GI-2290778 MADS box transcription factor 1 207 6.60E-22 3
G1760 GI-3912987 AGL8 SOLCO FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 207 6.60E-22 3
Table 2
G1760 GI-7446507 T07902 MADS box protein - Commerson's wild... 1 207 6.60E-22 3
G1760 GI-2507629 putative MADS box transcription factor PrM... 1 201 6.70E-22 2
G1760 GI-7446556 T10776 probable MADS box protein MADS6 - M... 1 201 6.70E-22 2
G1760 GI-5295982 MADS box-like protein 1 219 8.00E-22 2
G1760 GI-8574457 AF072534_1 pepper MADS-box protein [Capsic... 1 219 8.00E-22 2
G1760 GI-10188331 unnamed protein product [Zea mays] 1 208 8.30E-22 2
G1760 GI-13384054 AF335238_1 MADS-box transcription factor F... 1 206 8.40E-22 3
G1760 GI-1483232 MADS5 protein 1 198 8.80E-22 3
G1760 GI-13384056 AF335239_1 MADS-box transcription factor F... 1 218 1.00E-21 2
G1760 GI-13384052 AF335237_1 MADS-box transcription factor F... 1 215 1.00E-21 2
G1760 GI-3114588 MADS box protein 1 213 1.00E-21 3
G1760 GI-6468286 putative MADS domain transcription factor ... 1 214 1.00E-21 2
G1760 GI-13384060 AF335241_1 MADS-box transcription factor F... 1 207 1.10E-21 2
G1760 GI-4837612 farinelli protein 1 205 1.10E-21 2
G1760 GI-2507623 putative MADS box transcription factor PrM... 1 215 1.30E-21 2
G1760 GI-7446554 T10751 MADS-box protein MADS9 - Monterey pine 1 215 1.30E-21 2
G1760 GI-16549070 putative MADS-domain transcription factor ... 1 210 1.30E-21 2
G1760 GI-7328575 AF181479_1 MADS-box DNA binding protein [Z... 1 209 1.30E-21 2
G1760 GI-11494137 AF209729_1 putative MADS box transcription... 1 207 1.30E-21 2
G1760 GI-12002141 AF112150_1 MADS box protein 3 [Zea mays] 1 206 1.40E-21 2
G1760 GI-13448660 MADS box transcription factor [Ipomoea bat... 1 218 1.60E-21 2
G1760 G1-6651033 AF129875_1 MADS box transcription factor M... 1 207 1.70E-21 2
G1760 GI-9367311 MADS-box protein 7 [Hordeum vulgare] 1 207 1.70E-21 2
G1760 GI-3337237 putative MADS box transcription factor PrM... 1 202 1.70E-21 2
G1760 GI-7446557 T10778 probable MADS box protein MADS8 - M... 1 202 1.70E-21 2
G1760 GI-4218175 MADS-box protein, GSQUA1 1 199 1.80E-21 3
G1760 GI-431908 transcription factor 1 218 2.10E-21 2
G1760 GI-1076646 S46526 transcription factor - common tobacco 1 218 2.10E-21 2
G1760 GI-3114584 MADS box protein 1 216 2.10E-21 2
G1760 GI-609253 Saapl 1 194 2.30E-21 3
G1760 GI-1076477 S52236 Saapl protein - white mustard 1 194 2.30E-21 3
G1 60 GI-3913047 AP1_SINAL FLORAL HOMEOTIC PROTEIN APETALA1.. 1 194 2.30E-21 3
G1760 GI-2735764 MADS transcriptional factor; STMADS16 1 220 2.60E-21 2
G1760 GI-7446547 T06995 probable MADS box transcription fac... 1 220 2.60E-21 2
G1760 GI-6467976 AF198176_1 MADS box protein DOMADS3 1 202 2.80E-21 3
G1760 GI-6652756 MADS box protein 1 223 3.30E-21 2
Table 2
3 G1760 GI-313113 pMADS3 1 212 3.50E-21 2
3 G1760 GI-478387 JQ2212 pMADS3 protein - garden petunia 1 212 3.50E-21 2
3 G1760 GI-3913006 AG_PETHY AGAMOUS PROTEIN 1 212 3.50E-21 2
3 G1760 GI-17827467 PMADS3 [Petunia x hybrida] 1 212 3.50E-21 2
3 G1760 GI-13384066 AF335244_1 MADS-box transcription factor F... 1 212 3.50E-21 2
3 G1760 GI-13384058 AF335240_1 MADS-box transcription factor F... 1 208 3.50E-21 2
3 G1760 GI-695690 DAL3 protein 1 205 3.60E-21 2
3 G1760 GI-7484434 T14848 MADS-box protein dal3 - Norway spruce 1 205 3.60E-21 2
3 G1760 GI-2981612 transcription factor 1 226 4.00E-21 3
3 G1760 GI-1206003 putative MADS-box family transcription factor 1 213 4.40E-21 2
3 G1760 GI-7446558 T09569 MADS box protein MADS1 - Monterey pine 1 213 4.40E-21 2
3 G1760 GI-1049024 transcription factor SaMADS B 1 201 4.60E-21 2
3 G1760 GI-3913000 AGL8_SINAL FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 201 4.60E-21 2
3 G1760 GI-1049022 transcription factor SaMADS A 1 213 5.60E-21 2
3 G1760 GI-7446552 T10422 MADS box protein A - white mustard 1 213 5.60E-21 2
3 G1760 GI-3646326 MADS-box protein 1 210 5.60E-21 2
3 G1760 GI-5777906 U78950_1 MADS-box protein 4 1 210 5.60E-21 2
3 G1760 GI-939785 MADS box protein 1 203 5.80E-21 2 3 G1760 GI-7446509 T03410 MADS box protein - maize 1 203 5.80E-21 2
3 G1760 GI-11037010 AF305696_1 MADS-box protein EAP2S [Eucalyp... 1 195 6.00E-21 2
3 G1760 GI-11120557 AF306349_1 MADS box protein AP2L [Eucalypt... 1 195 6.00E-21 2
3 G1760 GI-13384050 AF335236_1 MADS-box transcription factor F... 1 210 7.20E-21 2
3 G1760 GI-9367315 MADS-box protein 9 [Hordeum vulgare] 1 207 7.20E-21 2
3 G1760 GI-2507627 putative MADS box transcription factor PrM... 1 212 9.00E-21 2
3 G1760 GI-7446555 T10767 probable MADS box protein MADS5 - M... 1 212 9.00E-21 2
3 G1760 GI-5230654 MADS-box protein 1 176 9.20E-21 4
3 G1760 GI-1905930 MADS box protein 1 208 9.20E-21 2
3 G1760 GI-2827300 MADS-box protein NMH 7 1 208 9.20E-21 2
3 G1760 GI-3493647 transcription activator 1 208 9.20E-21 2
3 G1760 GI-7446519 T04335 MADS box protein - rice 1 208 9.20E-21 2
3 G1760 GI-7446532 T09335 MADS-box protein NMH 7 - alfalfa 1 208 9.20E-21 2
3 G1760 GI-5051933 MADS-box protein FDRMADS8 1 202 9.40E-21 2
3 G1760 GI-19380 TDR3 1 219 1.10E-20 2
3 G1760 GI-542034 S23729 TDR3 protein - tomato 1 219 1.10E-20 2
3 G1760 GI-320596 JQ1690 floral binding protein 2 - garden p... 1 210 1.20E-20 2
3 G1760 GI-1181186 transcription factor 1 210 1.20E-20 2
Table 2
G1760 GI-1239961 MADS-box gene 1 210 1.20E-20 2
G1760 GI-1345965 FBP2_PETHY FLORAL HOMEOTIC PROTEIN FBP2 (F... 1 210 1.20E-20 2
G1760 GI-5070138 AF068722_1 MADS-box protein MADS3 1 210 1.20E-20 2
G1760 GI-7446512 S78015 MADS box protein DEFH49 - garden sn... 1 210 1.20E-20 2
G1760 GI-5295990 MADS box-like protein 1 207 1.20E-20 2
G1760 GI-1870206 MADS-box protein 1 202 1.20E-20 2
G1760 GI-18057092 AC092697_3 putative transcription factor [... 1 202 1.20E-20 2
G1760 GI-5295984 MADS box-like protein 1 201 1.20E-20 2
G1760 GI-2507625 putative MADS box transcription factor PrM... 1 196 1.20E-20 2
G1760 GI-7446564 T10764 probable MADS box protein MADS4 - M... 1 196 1.20E-20 2
G1760 GI-1905944 MADS box transcription factor SbMADSI 1 207 1.50E-20 2
G1760 GI-7446545 T14801 MADS box protein MADS1 - sorghum 1 207 1.50E-20 2
G1760 GI-2463333 M79 protein 1 211 1.90E-20 2
G1760 GI-7446518 T04307 M79 protein - rice 1 211 1.90E-20 2
G1760 GI-3290209 MADS-box protein 1 1 210 1.90E-20 2
G1760 GI-3646334 MdMADSβ 1 210 1.90E-20 2
G1760 GI-7488622 T17023 MADS box protein 1 - apple tree 1 210 1.90E-20 2
G1760 GI-19358 TDR5 1 208 1.90E-20 2
G1760 GI-19384 TDR5 1 208 1.90E-20 2
G1760 GI-629674 S23728 TDR5 protein - tomato 1 208 1.90E-20 2
G1760 GI-3913002 AGL9J.YCES FLORAL HOMEOTIC PROTEIN AGL9 HO... 1 208 1.90E-20 2
G1760 GI-695317 MADS box protein 1 206 1.90E-20 2
G1760 GI-3023536 CMB1_DIACA MADS BOX PROTEIN CMB1 1 206 1.90E-20 2
G1760 GI-7446563 T10714 MADS-box protein CMB1 - clove pink 1 206 1.90E-20 2
G1760 GI-6580947 AF158543_1 MADS-box transcription factor 1 205 1.90E-20 2
G1760 GI-12002139 AF112148_1 MADS box protein 1 [Zea mays] 1 205 1.90E-20 2
G1760 GI-6635740 AF207699_1 agamous-like MADS box protein O... 1 196 1.90E-20 3
G1760 GI-2286113 MADS box protein 1 207 2.40E-20 2
G1760 GI-7446544 T04170 MADS box protein - rice 1 207 2.40E-20 2
G1760 GI-642593 amino acid feature: MADS box; codes for a ... 1 198 2.50E-20 2
G1760 GI-7488599 T14457 MADS box protein homolog CAL - broc... 1 198 2.50E-20 2
G1760 GI-3947985 MADS-box protein 2 1 198 2.50E-20 2
G1760 GI-5295978 MADS box-like protein 1 196 2.50E-20 2
G1760 GI-7672991 AF144623_1 MADS-box transcription factor [... 1 215 3.00E-20 2
G1760 GI-161.7211 MADS D 1 210 3.00E-20 2
G1760 GI-3912986 AGL9_SINAL FLORAL HOMEOTIC PROTEIN AGL9 HO... 1 210 3.00E-20 2
Table 2
G1760 GI-7446553 T10467 MADS box protein D - white mustard 210 3.00E-20 2
G1760 GI-13384046 AF335234_1 MADS-box transcription factor F... 213 3.80E-20 2
G1760 GI-12964064 MADS box transcription factor [Oryza sativa] 211 3.80E-20 2
G1760 GI-12597207 putative MADS-box protein [Cucumis sativus] 209 3.90E-20 2
G1760 GI-16973294 B-type MADS box protein [Malus x domestica] 185 4.20E-20 2
G1760 GI-6580943 AF158541_1 MADS-box transcription factor 205 5.00E-20 2
G1760 GI-6580945 AF158542_1 MADS-box transcription factor 205 5.00E-20 2
G1760 GI-8567991 AF068723_1 MADS-box protein MADS4 [Nicotia.. 201 5.10E-20 2
G1760 GI-16549060 putative MADS-domain transcription factor ... 163 6.00E-20 3
G1760 GI-6580941 AF158540_1 MADS-box transcription factor 199 6.20E-20 3
G1760 GI-4433623 MADS-box transcription factor 209 6.20E-20 2
G1760 GI-6467972 AF198174_1 MADS box protein DOMADS1 209 6.20E-20 2
G1760 GI-6606072 AF058698_1 MADS15 protein 201 6.40E-20 2
G1760 GI-4218169 MADs-box protein, GDEF1 198 6.50E-20 2
G1760 GI-3184054 MADS-box transcription factor 205 8.10E-20 2
G1 60 GI-7446516 T06543 MADS box protein - garden pea 205 8.10E-20 2
G1760 GI-13446154 MADS-box transcription factor [Pisu sativum] 198 8.30E-20 2
G1760 GI-13661024 MADS-box transcription factor [Pisum sativum] 198 8.30E-20 2
G1760 GI-18252655 AF461740_1 MADS-box transcription factor M... 198 8.30E-20 2
G1760 GI-6580939 AF158539_1 MADS-box transcription factor 199 1.00E-19 3
G1760 GI-2286111 MADS box protein 204 1.00E-19 2
G1760 GI-7446543 T04169 MADS box protein - rice 204 1.00E-19 2
G1760 GI-8745070 MADS box protein [Betula pendula] 202 1.30E-19 2
G1760 GI-9367232 MADS box protein 1 [Hordeum vulgare] 206 1.60E-19 2
G1760 GI-9367234 MADS-box protein 1-2 [Hordeum vulgare] 206 1.60E-19 2
G1 60 GI-10803404 MADS box protein [Gerbera hybrida] 203 1.70E-19 2
G1760 GI-1370276 MADS-box protein 199 1.70E-19 2
G1760 GI-10835358 PTD protein [Populus balsamifera subsp. tr... 192 1.70E-19 2
G1760 GI-12655901 AF226865_1 putative transcription factor A... 207 2.10E-19 2
G1760 GI-17223670 SHATTERPROOF1 [Brassica napus] 207 2.10E-19 2
G1760 GI-6683777 AF135962_1 CAGL2 207 2.10E-19 2
G1760 GI-15808691 AF185574_1 transcription factor MAGL4 [Pop... 203 2.10E-19 2
G1760 GI-951172 MADS box protein 215 2.60E-19 2
G1760 GI-1001934 ZAG2 215 2.60E-19 2
G1760 GI-4105097 MADS box protein 26 210 2.60E-19 2
G1760 GI-22665 GP (green petal) 199 2.70E-19 2
Table 2
3 G1760 GI-322773 S31693 transcription factor gp - garden pe... 199 2.70E-19 2
3 G1760 GI-729974 MAD1_PETHY FLORAL HOMEOTIC PROTEIN PMADS1 ... 199 2.70E-19 2
3 G1760 GI-3646322 MADS-box protein 201 3.50E-19 2
3 G1760 GI-13442962 AF095646_1 MADS box protein nmads3 [Oryza ... 184 3.70E-19 2
3 G1 60 GI-13384048 AF335235_1 MADS-box transcription factor F... 201 4.40E-19 2
3 G1760 GI-431226 deficiens analogue 195 4.50E-19 2
3 G1760 GI-511065 deficiens analogue 195 4.50E-19 2
3 G1760 GI-7446533 T07410 MADS box protein homolog DEF2 - potato 195 4.50E-19 2
3 G1760 GI-7446549 T07066 MADS-box protein homolog DEF4 - potato 195 4.50E-19 2
3 G1760 GI-8163950 AF230704_1 MADS box transcription factor T... 195 4.50E-19 2
3 G1760 GI-1561780 homeotic protein boi2AP1 193 4.50E-19 2
3 G1760 GI-4416347 MADS C-2 protein; MADS-box protein 193 4.50E-19 2
3 G1760 GI-1561778 homeotic protein boil AP1 192 5.80E-19 2
3 G1760 GI-8163952 AF230705_1 MADS box transcription factor A... 189 5.80E-19 2
3 G1760 GI-1905934 MADS box protein 181 6.00E-19 2
3 G1760 GI-7489647 T14737 MADS box protein - sorghum (fragment) 181 6.00E-19 2
3 G1760 GI-2735766 MADS transcriptional factor; STMADS11 207 6.90E-19 2 vβ 3 G1760 GI-7446550 T06996 MADS-box transcription factor MADS1... 207 6.90E-19 2
° 3 G1760 GI-1001935 ZMM1 214 8.60E-19 2
3 G1760 GI-1167914 MADS box protein 214 8.60E-19 2
3 G1760 GI-7446525 T02261 MADS box protein - maize 214 8.60E-19 2
3 G1760 GI-18650789 AF234617_1 MADS-box transcription factor [... 213 8.60E-19 2
3 G1760 GI-15022157 MADS box protein-like protein NGL9 [Medica... 188 8.90E-19 3
3 G1760 GI-17933456 MADS-box protein [Brassica napus] 193 1.10E-18 3
3 G1760 GI-3851331 putative MADS-domain transcription factor 168 1.30E-18 2
3 G1760 GI-2981614 transcription factor 203 1.40E-18 2
3 G1760 GI-3646336 MdMADS9 187 1.50E-18 2
3 G1760 GI-1483228 MADS3 protein 194 1.80E-18 3
3 G1760 GI-8216957 putative transcription factor [Cucumis sat... 193 1.90E-18 2
3 G1760 GI-3892652 putative MADS-domain transcription factor 166 2.10E-18 2
3 G1760 GI-15667640 AF097747_1 putative MADS-box family transc... 213 2.20E-18 2
3 G1760 GI-2529340 homologue; putative 209 2.30E-18 2
3 G1760 GI-7446524 T01700 hypothetical protein - maize 209 2.30E-18 2
3 G1760 GI-13274182 putative MADS-domain transcription factor ... 235 2.60E-18 1
3 G1760 GI-1561782 homeotic protein boil AP3 193 3.10E-18 2
3 G1760 GI-6841082 AF124814_1 APETALA3 193 3.10E-18 2
Table 2
G1760 GI-4096982 AGAMOUS protein 1 206 3.70E-18 2
G1760 GI-5616513 AF168468_1 agamous protein 1 206 3.70E-18 2
G1760 GI-6970413 MADS-box protein 1 206 3.70E-18 2
G1760 GI-6970415 MADS-box protein 1 206 3.70E-18 2
G1760 GI-6970417 MADS-box protein 1 206 3.70E-18 2
G1760 GI-13384064 AF335243_1 MADS-box transcription factor F... 1 196 3.90E-18 2
G1760 GI-886401 MADS box protein 1 233 4.20E-18 1
G1760 GI-7446534 T03894 MADS box protein - rice 1 233 4.20E-18 1
G1760 GI-13272279 MADS [Oryza sativa] 1 233 4.20E-18 1
G1760 GI-15667638 AF097746_1 putative MADS-box family transc... 1 218 4.50E-18 2
G1760 GI-3986689 MADS box protein 1 217 4.50E-18 2
G1760 GI-13274178 putative MADS-domain transcription factor ... 1 232 5.40E-18 1
G1760 GI-13274180 putative MADS-domain transcription factor ... 1 231 6.90E-18 1
G1760 GI-5051935 MADS-box protein FDRMADS1 1 180 8.50E-18 2
G1760 GI-19388 TDR8 1 211 9.50E-18 2
G1760 GI-542036 S23732 TDR8 protein - tomato 1 211 9.50E-18 2
G1760 GI-6580949 AF158544_1 MADS-box transcription factor 1 205 9.80E-18 2
G1760 GI-2293894 unnamed protein product 1 181 1.40E-17 2
G1760 GI-16549083 putative MADS-domain transcription factor ... 1 168 1.40E-17 2
G1760 GI-14041687 putative agamous protein [Juglans regia] 1 138 1.50E-17 3
G1760 GI-602902 SLM2 1 196 1.60E-17 2
G1760 GI-1561786 homeotic protein boi2AP3 1 193 1.70E-17 2
G1760 GI-7446540 T14473 MADS box protein 2AP3 - broccoli 1 193 1.70E-17 2
G1760 GI-695319 MADS box protein 1 189 1.70E-17 2
G1760 GI-722400 MADS box protein 1 189 1.70E-17 2
G1760 GI-3023537 CMB2_DIACA MADS BOX PROTEIN CMB2 1 189 1.70E-17 2
G1760 GI-7446538 T10715 MADS-box protein CMB2 - clove pink 1 189 1.70E-17 2
G1760 GI-16549062 putative MADS-domain transcription factor ... 1 175 2.20E-17 2
G1760 GI-16018 DEF A protein 1 188 2.70E-17 2
G1760 GI-16020 deficiens 1 188 2.70E-17 2
G1760 GI-100479 S12378 defA-1 protein - garden snapdragon 1 188 2.70E-17 2
G1760 GI-100483 S19232 gene deficiens protein - garden sna... 1 188 2.70E-17 2
G1760 GI-118426 DEFA_ANTMA FLORAL HOMEOTIC PROTEIN DEFICIENS 1 188 2.70E-17 2
G1760 GI-16549081 putative MADS-domain transcription factor ... 1 173 2.90E-17 2
G1760 GI-14518447 AF130118_1 MADS box protein [Capsicum annuum] 1 207 5.20E-17 2
G1760 GI-3170512 APETALA3 homolog PhAP3 1 169 7.60E-17 2
Table 2
3 G1760 GI-10880313 putative MADS-domain transcription factor ... 1 221 8.10E-17 1
3 G1760 GI-4102111 NAP1-1 1 204 8.50E-17 2
3 G1760 GI-1621333 homeotic protein bobap3 1 186 9.10E-17 2
3 G1760 GI-7446541 T14474 MADS box protein ap3 - broccoli 1 186 9.10E-17 2
3 G1760 GI-19386 TDR6 1 174 1.50E-16 2
3 G1760 GI-481512 S38778 TDR6 protein - tomato (fragment) 1 174 1.50E-16 2
3 G1760 GI-542029 S23731 TDR6 protein - tomato (fragment) 1 174 1.50E-16 2
3 G1760 GI-6606306 AF176783_1 floral binding protein 26 1 201 1.80E-16 2
3 G1760 GI-15216293 MADS-box protein [Rosa rugosa] 1 185 1.80E-16 2
3 G1760 G1-3253147 CMADS1 1 214 2.50E-16 3
3 G1760 GI-7677036 FDRMADS7 [Oryza sativa] 1 163 2.80E-16 3
3 G1760 GI-790637 AGL15 1 215 3.60E-16 1
3 G1760 GI-1362199 555773 homeotic protein ZAG2 - maize (frag... 1 215 3.60E-16 1
3 G1760 GI-2961437 MADS box protein 1 215 3.60E-16 1
3 G1760 GI-3170464 APETALA3 homolog PnPI-1 1 215 3.60E-16 1
3 G1760 GI-3831486 AG15_BRANA FLORAL HOMEOTIC PROTEIN AGL15 1 215 3.60E-16 1
3 G1760 GI-7446535 T03902 MADS4 box protein - rice 1 215 3.60E-16 1 vβ 3 G1760 GI-7446560 T07867 MADS box protein AGL15 (type 1) - rape 1 215 3.60E-16 1
M 3 G1760 GI-1362200 555774 homeotic protein ZMM1 - maize (frag... 1 214 4.50E-16 1
3 G1760 GI-5070142 AF068725_1 MADS-box protein MADS1 1 198 4.60E-16 2
3 G1760 GI-13442960 AF095645_1 MADS box protein nmadsl [Oryza ... 1 198 4.60E-16 2
3 G1760 GI-3114586 MADS box protein 1 213 5.80E-16 1
3 G1760 GI-2981610 transcription factor 1 189 6.10E-16 2
3 G1760 GI-6470126 AF151693_1 transcription factor 1 212 7.40E-16 1
3 G1760 GI-19871 NTGLOBOSA 1 210 1.20E-15 1
3 G1 60 GI-169254 transcription factor 1 210 1.20E-15 1
3 G1 60 GI-320595 JQ1689 floral binding protein 1 - garden p... 1 210 1.20E-15 1
3 G1760 GI-417063 GLOB TOBAC FLORAL HOMEOTIC PROTEIN GLOBOSA 1 210 1.20E-15 1
3 G1760 GI-448288 1916408A NTGLO gene [Nicotiana tabacum] 1 210 1.20E-15 1
3 G1760 GI-486750 S35226 homeotic protein globosa homolog - ... 1 210 1.20E-15 1
3 G1760 GI-729464 FBP1_PETHY FLORAL HOMEOTIC PROTEIN FBP1 (F... 1 210 1.20E-15 1
3 G1760 GI-2293892 unnamed protein product 1 210 1.20E-15 1
3 G1760 GI-17933454 MADS-box protein [Brassica napus] 1 195 1.20E-15 2
3 G1760 GI-6092007 GpMADSI 1 178 1.30E-15 2
3 G1760 GI-16024 globosa 1 207 2.50E-15 1
3 G1760 GI-282981 S28062 homeotic protein globosa - garden s... 1 207 2.50E-15 1
Table 2
3 G1760 GI-417062 GLOB_ANTMA FLORAL HOMEOTIC PROTEIN GLOBOSA 1 207 2.50E-15 1
3 G1760 GI-3851333 putative MADS-domain transcription factor 1 168 2.80E-15 2
3 G1760 GI-22667 PMADS2 1 206 3.20E-15
3 G1760 GI-322775 S31707 transcription factor pmads2 - garde... 1 206 3.20E-15
3 G1760 GI-454265 FBP3 1 206 3.20E-15
3 G1760 GI-729976 MAD2_PETHY FLORAL HOMEOTIC PROTEIN PMADS 2 1 206 3.20E-15
3 G1760 GI-1362203 S55938 homeotic protein ZMM4 - maize (frag... 1 206 3.20E-15
3 G1760 GI-2129971 S60288 FBP3 protein - garden petunia 1 206 3.20E-15
3 G1760 GI-12666533 Pistillata MADS-box protein [Malus x domes... 1 206 3.20E-15
3 G1760 GI-12666535 Pistillata MADS-box protein [Malus x domes... 1 206 3.20E-15
3 G1760 GI-1418272 AGL15 type 2 1 205 4.20E-15
3 G1760 GI-4885034 AF134114_1 PISTILLATA protein homolog HPH 1 205 4.20E-15
3 G1760 GI-7446561 T07869 MADS box protein AGL15 (type 2) - rape 1 205 4.20E-15
3 G1760 GI-6468288 putative MADS domain transcription factor ... 1 183 4.20E-15 2
3 G1760 GI-16549064 putative MADS-domain transcription factor ... 1 166 4.80E-15 3
3 G1760 GI-4218173 MADS-box protein, GGL01 1 204 5.30E-15
3 G1760 GI-17432174 MADS-box protein [Lycopersicon esculentum] 1 204 5.30E-15 vβ 3 G1760 GI-2055376 MADS box protein 1 170 6.60E-15 2 w 3 G1760 GI-1362196 S55772 homeotic protein TMZ1-I15a - maize ... 1 202 8.70E-15
3 G1760 GI-1362198 S55768 homeotic protein ZAG1 - maize (frag... 1 202 8.70E-15
3 G1760 GI-4885036 AF134115_1 PISTILLATA protein homolog HPI2 1 202 8.70E-15
3 G1760 GI-309574 homologue of Arabidopsis gene AGAMOUS 1 202 8.80E-15 2
3 G1760 GI-542191 JQ2289 floral homeotic protein ZAG1 - maize 1 202 8.80E-15 2
3 G1760 GI-5764255 AF147243_1 ASAPETALA3/TM6-B 1 154 9.60E-15 2
3 G1760 GI-5764279 AF147255_1 ASAPETALA3/TM6 1 154 1.20E-14 2
3 G1760 GI-10880319 putative MADS-domain transcription factor ... 1 154 1.20E-14 2
3 G1760 GI-939783 MADS box protein 1 200 1.40E-14 1
3 G1760 GI-7489788 T03409 MADS box protein - maize (fragment) 1 200 1.40E-14 1
3 G1760 GI-8163966 AF230712_1 MADS box containing protein PI ... 1 200 1.40E-14 1
3 G1760 GI-5764275 AF147253_1 ASAPETALA3/TM6 1 155 1.50E-14 2
3 G1760 GI-5764235 AF147233_1 ASAPETALA3 TM6-A 1 153 1.60E-14 2
3 G1760 GI-3253149 CMADS2 1 199 1.80E-14 1
3 G1760 GI-3170462 APETALA3 homolog RfAP3-1 1 161 1.90E-14 2
3 G1760 GI-5764257 AF147244_1 ASAPETALA3/TM6-B 1 154 2.00E-14 2
3 G1760 GI-5764237 AF147234_1 ASAPETALA3 TM6-A 1 154 2.00E-14 2
3 G1 60 GI-5764241 AF147236_1 ASAPETALA3/TM6-A 1 154 2.00E-14 2
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CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO Table 2
3 G1760 GI-5764247 AF147239_1 ASAPETALA3/TM6-A 150 5.20E-14 2
3 G1760 GI-5764261 AF147246_1 ASAPETALA3/TM6-B 150 5.20E-14 2
3 G1760 GI-887577 MADS box regulatory protein 178 7.60E-14 2
3 G1760 GI-1046274 MADS box regulatory protein 178 7.60E-14 2
3 G1760 GI-2507631 putative MADS box transcription factor PrM... 193 8.00E-14
3 G1760 GI-5295988 MADS box-like protein 193 8.00E-14
5 G1820 AW776719 AW776719 EST335784 DSIL Medicago truncatula ... 3 461 2.20E-41
5 G1820 BF005772 BF005772 EST434270 DSLC Medicago truncatula ... 3 440 4.30E-39
5 G1820 BG591677 BG591677 EST499519 P. infestans-challenged I... 2 430 3.90E-38
5 G1820 BI701620 BI701620 sai18a04.y1 Gm-c1053 Glycine max cD... 3 417 1.20E-36
5 G1820 BH725354 BH725354 BOHV037TF BO_2_3_KB Brassica olerac... 2 406 1.40E-35
5 G1820 BG594850 BG594850 EST493528 cSTS Solanum tuberosum cD... 1 400 6.80E-35
5 G1820 AW093662 AW093662 EST286842 tomato mixed elicitor, BT... 2 392 4.80E-34
5 G1820 BE208917 BE208917 GF-FV-P3F5 Marsh grapefruit young f... 2 391 5.60E-34
5 G1820 BM309697 BM309697 sak65e12.y1 Gm-c1036 Glycine max cD... 2 391 6.80E-34
5 G1820 BE460825 BE460825 EST412244 tomato breaker fruit, TIG... 1 390 8.40E-34
5 G1820 BG440805 BG440805 GA Ea0010D12f Gossypium arboreum 1... 1 387 1.50E-33
AI490736 EST241444 tomato shoot, Cornell Lye... vβ 5 G1820 AI490736 2 249 1.70E-33
« 5 G1820 AW216620 AW216620 EST295334 tomato callus, TAMU Lycop... 2 385 2.90E-33
5 G1820 BF473402 BF473402 WHE0924_A08_B16ZS Wheat 5-15 DAP sp... 1 379 1.10E-32
5 G1820 AW625757 AW625757 EST319664 tomato radicle, 5 d post-... 3 379 1.30E-32
5 G1820 BM816719 BM816719 HB02A06_T3.ab1 HB Hordeum vulgare c... 1 377 1.40E-32
5 G1820 BI307850 BI307850 EST529260 GPOD Medicago truncatula ... 2 376 1.80E-32
5 G1820 BE053572 BE053572 GA Ea0004E08f Gossypium arboreum 7... 3 376 1.90E-32
5 G1820 AP004113 AP004113 Oryza sativa chromosome 2 clone OJ1... -3 382 3.50E-32
5 G1820 AW706553 AW706553 sj58h11.y1 Gm-c1033 Glycine max cDN... 1 374 4.40E-32
5 G1820 BE461445 BE461445 EST412864 tomato breaker fruit, TIG... 2 374 4.50E-32
5 G1820 AU197697 AU197697 AU197697 Rice mature leaf Oryza sat... 3 368 2.30E-31
5 G1820 AX180963 AX180963 Sequence 14 from Patent WO0145493. ... 1 364 2.40E-31
5 G1820 AW223272 AW223272 EST300083 tomato fruit red ripe, TA... 2 366 3.00E-31
5 G1820 BG351673 BG351673 130F10 Mature tuber lambda ZAP Sola... 1 364 3.50E-31
5 G1820 BI935071 BI935071 EST554960 tomato flower, anthesis L... 1 364 3.70E-31
5 G1820 BI934205 BI934205 EST554094 tomato flower, anthesis L... 1 364 3.70E-31
5 G1820 BH679015 BH679015 BOHX096TF BO_2_3_KB Brassica olerac... 2 363 4.30E-31
5 G1820 BI273406 BI273406 949024F03.y1 949 - Juvenile leaf an... 1 364 4.80E-31
5 G1820 BG593806 BG593806 EST492484 cSTS Solanum tuberosum cD... 3 363 4.90E-31
LJJ IJJ LJJ LJJ LLJ L LLI LJJ LJJ LLI LJJ LJJ o o o o o o σσ oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo o o o o o o o o o o o
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o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ Table 2
G1820 BF154242 BF154242 057E07 Mature tuber lambda ZAP Sola... 2 338 2.50E-28 1
G1820 AL384854 AL384854 MtBC24H07F1 MtBC Medicago truncatul... 1 339 2.80E-28 1
G1820 BF646855 BF646855 NF075D07EC1 F1061 Elicited cell cult... 3 337 3.10E-28 1
G1820 AW691165 A 691165 NF041 G11 ST1 F1000 Developing stem Me... 3 337 3.20E-28 1
G1820 BF645105 BF645105 NF028G05EC1F1039 Elicited cell cult... 2 337 3.20E-28 1
G1820 BF645348 BF645348 NF039B08EC1 F1064 Elicited cell cult... 3 337 3.60E-28 1
G1820 BM306993 BM306993 sak35c03.y1 Gm-c1075 Glycine max cD... 3 337 3.70E-28 1
G1820 BE494994 BE494994 WHE1273_A04_B07ZS Secale cereale an... 1 337 3.70E-28 1
G1820 BE499690 BE499690 WHE0959_D11_G21ZS Wheat pre-anthesi... 2 337 4.30E-28 1
G1820 BG359797 BG359797 sac28f07.y1 Gm-c1051 Glycine max cD... 2 336 5.70E-28 1
G1820 AW471965 AW471965 si18c05.y1 Gm-c1029 Glycine max cDN... 1 335 6.30E-28 1
G1820 AW098949 AW098949 ga09c09.y1 Moss EST library CPU Cer... 3 334 9.80E-28 1
G1820 BF646992 BF646992 NF026D12EC1 F1102 Elicited cell cult... 2 331 1.30E-27 1
G1820 BF649720 BF649720 NF082E03EC1 F1021 Elicited cell cult... 2 330 1.70E-27 1
G1820 BF645269 BF645269 NF034B11 EC1 F1092 Elicited cell cult... 3 330 1.70E-27 1
G1820 BI397827 BI397827 NXPV_108_B12_F NXPV (Nsf Xylem Plan... 1 331 1.80E-27 1
G1820 BG597998 BG597998 EST496676 cSTS Solanum tuberosum cD... 3 330 1.80E-27 1
G1820 BG318104 BG318104 NXPV_010_E08_F NXPV (Nsf Xylem Plan... 2 330 2.20E-27 1
G1820 BE428397 BE428397 MTD006.E11 F990616 ITEC MTD Durum Wh. 1 330 3.00E-27 1
G1820 BG448089 BG448089 NF105F11EC1F1093 Elicited cell cult... 2 327 3.60E-27 1
G1820 BG098467 BG098467 EST462986 sprouting eyes/shoots Sol... 2 326 4.50E-27 1
G1820 AW773893 AW773893 EST332879 KV3 Medicago truncatula c... 2 326 4.70E-27 1
G1820 BI180116 BI180116 EST521061 cSTE Solanum tuberosum cD... 2 326 4.80E-27 1
G1820 BG592807 BG592807 EST491485 cSTS Solanum tuberosum cD... 3 326 4.80E-27 1
G1820 BG889790 BG889790 EST515641 cSTD Solanum tuberosum cD... 3 326 4.80E-27 1
G1820 BG597856 BG597856 EST496534 cSTS Solanum tuberosum cD... 3 325 6.20E-27 1
G1820 BG050028 BG050028 FM1_65_E12.b1_A003 Floral-Induced M... 3 324 8.90E-27 1
G1820 BG454495 BG454495 NF109H12LF1 F1101 Developing leaf Me... 1 240 1.40E-26 2
G1820 BI405998 BI405998 150D05 Mature tuber lambda ZAP Sola... 3 320 1.90E-26 1
G1820 BG594241 BG594241 EST492919 cSTS Solanum tuberosum cD... 1 320 2.30E-26 1
G1820 BE055336 BE055336 GA Ea0031 l15f Gossypium arboreum 1... 2 317 2.40E-26 1
G1820 BE659925 BE659925 535 GmaxSC Glycine max cDNA, mRNA s... 2 318 2.60E-26 1
G1820 BM085845 BM085845 sah32c04.y1 Gm-c1036 Glycine max cD... 1 316 6.10E-26 1
G1820 BI436125 BI436125 EST538886 cSTD Solanum tuberosum cD... 2 312 1.60E-25 1
G1820 BE202650 BE202650 EST402672 KV1 Medicago truncatula c... 2 310 2.60E-25 1
G1820 AV416903 AV416903 AV416903 Lotus japonicus young plan... 3 309 5.10E-25 1
Table 2
5 G1820 BG644364 BG644364 EST505983 KV3 Medicago truncatula c... 2 185 8.00E-25 2
5 G1820 AW220025 AW220025 EST302508 tomato root during/after ... 3 306 9.60E-25 1
5 G1820 AW981474 AW981474 EST392627 DSIL Medicago truncatula ... 2 303 1.40E-24 1
5 G1820 BE343425 BE343425 EST408587 potato stolon, Cornell Un... 2 301 2.40E-24 1
5 G1820 BE920971 BE920971 EST424740 potato leaves and petiole... 2 301 2.40E-24 1
5 G1820 BM177728 BM177728 saj64g11.y1 Gm-c1072 Glycine max cD... 2 300 3.10E-24 1
5 G1820 AP003875 AP003875 Oryza sativa chromosome 8 clone OJ1... -1 305 5.00E-24 1
5 G1820 BG447405 BG447405 GA Eb0042K16f Gossypium arboreum 7... 3 169 6.10E-24 2
5 G1820 BF643237 BF643237 NF002F05EC1 F1044 Elicited cell cult... 3 296 7.90E-24 1
5 G1820 BE204855 BE204855 EST397595 KVO Medicago truncatula c... 2 295 1.00E-23 1
5 G1820 BM266390 BM266390 MEST380-G10.T3 ISUM5-RN Zea mays cD... -1 290 2.80E-23 1
5 G1820 AW934284 AW934284 EST360127 tomato fruit mature green... 3 290 3.20E-23 1
5 G1820 BM094604 BM094604 saj18b07.y1 Gm-c1066 Glycine max cD... 1 289 4.40E-23 1
5 G1820 BF459642 BF459642062F03 Mature tuber lambda ZAP Sola... 3 288 4.80E-23 1
5 G1820 BI262781 BI262781 NF091 F07EC1 F1063 Elicited cell cult... 3 287 6.50E-23 1
5 G1820 AW755787 AW755787 sl09d03.y1 Gm-c1036 Glycine max cDN... 1 287 7.00E-23 1
5 G1820 BE601397 BE601397 PI1_92_E01.g1_A002 Pathogen induced... 2 286 8.20E-23 1
G1820 AW671865 AW671865 LG1_352_F08.b1_A002 Light Grown 1 (... 1 286 8.90E-23 1 vβ 5
∞ 5 G1820 AW671138 AW671138 LG1_329_C03.b1_A002 Light Grown 1 (... 3 286 9.10E-23 1
5 G1820 BF266898 BF266898 HV_CEa0016F21f Hordeum vulgare seed... 1 286 9.10E-23 1
5 G1820 BG363124 BG363124 sac10f05.y1 Gm-c1040 Glycine max cD... 3 285 1.20E-22 1
5 G1820 Bl176786 BI176786 EST517731 cSTE Solanum tuberosum cD... 3 283 2.10E-22 1
5 G1820 BI397603 BI397603 NXPV_101_G06_F NXPV (Nsf Xylem Plan... 2 283 2.30E-22 1
5 G1820 BG445007 BG445007 GA Ea0026G17f Gossypium arboreum 7... 1 278 4.10E-22 1
5 G1820 BM888638 BM888638 952067B07.y1 952 - BMS tissue from ... -2 278 7.50E-22 1
5 G1820 BE823184 BE823184 GM700020A20E2 Gm- 070 Glycine max ... -2 276 9.20E-22 1
5 G1820 BF324752 BF324752 su27c08.y1 Gm-c1068 Glycine max cDN... 2 277 1.30E-21 1
5 G1820 BE807665 BE807665 ss28g07.y1 Gm-c1061 Glycine max cDN... 2 273 1.90E-21 1
5 G1820 BG597314 BG597314 EST495992 cSTS Solanum tuberosum cD... 3 139 2.80E-21 3
5 G1820 BE055056 BE055056 GA Ea0031 H16f Gossypium arboreum 7... 2 268 4.20E-21 1
5 G1820 AW030860 AW030860 EST274150 tomato callus, TAMU Lycop... 3 268 7.10E-21 1
5 G1820 BI436331 BI436331 EST539092 cSTE Solanum tuberosum cD... 3 268 7.10E-21 1
5 G1820 BE761883 BE761883 NXCI_071_A10_F NXCI (Nsf Xylem Comp... 3 266 1.90E-20 1
5 G1820 BM887238 BM887238 sam36a03.y1 Gm-c1068 Glycine max cD... 1 258 8.90E-20 1
5 G1820 AW906494 AW906494 EST342616 potato stolon, Cornell Un... 3 257 1.50E-19 1
5 G1820 BF651035 BF651035 NF109H05EC1 F1046 Elicited cell cult... 3 254 2.40E-19 1
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5 G1820 BE427144 BE427144 PSR6660 ITEC PSR Wheat Endosperm Li... 1 200 1.10E-13
5 G1820 BE431012 BE431012 SUN010.C12F991222 ITEC SUN Wheat cD... 2 199 1.50E-13
5 G1820 AI898409 AI898409 EST267852 tomato ovary, TAMU Lycope... 3 198 2.10E-13
5 G1820 BG452005 BG452005 NF043G11 DT1 F1085 Drought Medicago t... 1 194 4.60E-13
5 G1820 BH607026 BH607026 BOHBS38TR BOHB Brassica oleracea ge... -2 195 5.00E-13
5 G1820 AP003852 AP003852 Oryza sativa genomic DNA, chromosom... 1 199 8.60E-13
5 G1820 AP003205 AP003205 Oryza sativa genomic DNA, chromosom... 3 199 8.60E-13
5 G1820 BF484919 BF484919 WHE2333_B05_C09ZS Wheat pre-anthesi... 1 192 9.20E-13
5 G1820 B 108548 BM108548 EST556084 potato roots Solanum tube... 2 191 9.50E-13
5 G1820 AP003546 AP003546 Oryza sativa genomic DNA, chromosom... -3 197 1.40E-12
5 G1820 AP003610 AP003610 Oryza sativa genomic DNA, chromosom... 3 196 1.80E-12
5 G1820 AP003727 AP003727 Oryza sativa genomic DNA, chromosom... 2 196 1.80E-12
5 G1820 OSJN00074 AL606641 Oryza sativa chromosome 4 clone OSJ... -2 194 2.90E-12
5 G1820 BE922298 BE922298 EST426067 potato leaves and petiole... 1 189 3.60E-12
5 G1820 BE413990 BE413990 SCU005.B05.R990714 ITEC SCU Wheat E... 3 181 1.00E-11
5 G1820 AW267548 AW267548 618060D04.x1 618 - Inbred Tassel cD... -3 182 1.20E-11
5 G1820 BH140496 BH140496 ZMMBBb0002F23f Maize B73 Zea mays g... -1 175 4.70E-11
5 G1820 BE416817 BE416817 MUG014.B11 R990304 ITEC MUG Wheat Sp... 1 175 6.20E-11 o o 5 G1820 AU082303 AU082303 AU082303 Rice callus Oryza sativa c... 2 172 1.10E-10
5 G1820 AC097278 AC097278 Oryza sativa chromosome 10 clone OS... -2 179 1.10E-10
5 G1820 B 376816 BM376816 EBem05_SQ003_F04_R IGF Barley EBemO... 1 172 1.40E-10
5 G1820 H0719D04 AL442106 Oryza sativa chromosome 4 clone H07... -1 177 1.80E-10
5 G1820 AW394536 AW394536 sh32c06.y1 Gm-c1017 Glycine max cDN... 2 170 2.10E-10
5 G1820 AF074781 AF074781 AF074781 UVR lab, Indian Institute ... 2 163 7.60E-10
5 G1820 BI808608 BI808608 D004F12 Oryza sativa mature leaf Ii... -1 164 8.40E-10
5 G1820 BF634777 BF634777 NF070B06DT1 F1048 Drought Medicago t... 3 162 1.10E-09
5 G1820 BJ181866 BJ181866 BJ181866 normalized full length cDN... 3 161 2.40E-09
5 G1820 BG275344 BG275344 NXSI_142_F10_F NXSI (Nsf Xylem Side... 1 157 5.50E-09
5 G1820 AW671931 AW671931 LG1_353_C04.b1_A002 Light Grown 1 (... 2 152 1.60E-08
5 G1820 BM098827 BM098827 EBpi05_SQ002_C21_R IGF Barley EBpiO... 2 153 1.80E-08
5 G1820 B 098828 BM098828 EBpi05_SQ002_C22_R IGF Barley EBpiO... 2 153 1.80E-08
5 G1820 BG098468 BG098468 EST462987 sprouting eyes/shoots Sol... 2 148 4.50E-08
5 G1820 BH488102 BH488102 BOGLC34TF BOGL Brassica oleracea ge... -1 147 1.20E-07
5 G1820 BI205030 BI205030 EST523070 cTOS Lycopersicon esculen... 1 143 1.30E-07
5 G1820 BE418855 BE418855 SCL083.B09R990809 ITEC SCL Wheat Le... 3 143 1.50E-07
5 G1820 BM779158 BM779158 EST589733 KV2 Medicago truncatula c... 2 142 1.60E-07
Table 2
G1820 BG049792 BG049792 FM1_65_E12.g1_A003 Floral-Induced M... 3 142 1.80E-07 1
G1820 AW313181 AW313181 707040H08.X2 707 - Mixed adult tiss... -1 143 1.80E-07 1
G1820 BF647160 BF647160 NF031H02EC1F1027 Elicited cell cult... 1 142 2.10E-07 1
G1820 AW560428 AW560428 EST315476 DSIR Medicago truncatula ... 1 140 2.90E-07 1
G1820 BM113066 BM113066 EST560602 potato roots Solanum tube... 3 139 3.80E-07 1
G1820 AW559867 AW559867 EST314915 DSIR Medicago truncatula ... 1 138 4.60E-07 1
G1820 BE342548 BE342548 EST395392 potato stolon, Cornell Un... 3 136 7.70E-07 1
G1820 AU085270 AU085270 AU085270 Cryptomeria japonica inner... 2 136 8.40E-07 1
G1820 AW922171 AW922171 LG1_346_C02.b1_A002 Light Grown 1 (... 3 136 8.70E-07 1
G1820 BF586620 BF586620 FM1_29_E06.b1_A003 Floral-Induced M... 1 136 9.50E-07 1
G1820 BI972434 BI972434 sai77e01.y1 Gm-c1065 Glycine max cD... 1 135 9.50E-07 1
G1820 BF518963 BF518963 EST456356 DSIL Medicago truncatula ... 2 135 1.00E-06 1
G1820 AU030557 AU030557 AU030557 Rice cDNA from immature le... 2 136 1.10E-06 1
G1820 BI948741 BI948741 HVSMEI0010K21f Hordeum vulgare spik... 2 144 1.20E-06 1
G1820 BI422403 BI422403 EST533069 tomato callus, TAMU Lycop... 2 134 1.20E-06 1
G1820 BF635384 BF635384 NF064G07DT1 F1055 Drought Medicago t... 3 142 1.50E-06 1
G1820 BI425279 BI425279 saf13d11.y3 Gm-c1076 Glycine max cD... 2 135 1.70E-06 1
G1820 BM779165 BM779165 EST589740 KV2 Medicago truncatula c... 1 142 1.80E-06 1
G1820 BE659926 BE659926 985 GmaxSC Glycine max cDNA, mRNA s... 2 140 2.00E-06 1
G1820 BM360460 BM360460 GA Ea0031115r Gossypium arboreum 7... 1 132 2.20E-06 1
G1820 BE530941 BE530941 OC03D11 OC Oryza sativa cDNA clone ... 3 132 2.30E-06 1
G1820 BF004023 BF004023 EST432521 KV1 Medicago truncatula c... 2 142 2.40E-06 1
G1820 AI8991 7 AI899177 EST268620 tomato ovary, TAMU Lycope... 3 132 2.50E-06 1
G1820 AI485571 AI485571 EST243892 tomato ovary, TAMU Lycope... 2 130 3.30E-06 1
G1820 BF479507 BF479507 L48-3184T3 Ice plant Lambda Uni-Zap... 2 140 3.60E-06 1
G1820 AW443978 AW443978 EST308908 tomato mixed elicitor, BT... 2 135 3.80E-06 1
G1820 BE922002 BE922002 EST425771 potato leaves and petiole... 1 130 3.90E-06 1
G1820 BE609403 BE609403 so06f12.y1 Gm-c1035 Glycine max cDN... 3 130 4.00E-06 1
G1820 C99636 C99636 C99636 Rice panicle (longer than 10cm... -3 129 4.90E-06 1
G1820 AW455268 AW455268 EST311928 tomato root during/after ... 2 133 5.00E-06 1
G1820 BE417330 BE417330 MUG019.B03R990612 ITEC MUG Wheat Sp... 1 129 5.00E-06 1
G1820 BG645448 BG645448 EST507067 KV3 Medicago truncatula c... 1 140 6.20E-06 1
G1820 AW011641 AW011641 614011C01.y1 614 - root cDNA librar... 1 134 7.30E-06 1
G1820 BG881940 BG881940 sae91g02.y1 Gm-c1065 Glycine max cD... 2 127 7.90E-06 1
G1820 BE471702 BE471702 EST416555 potato stolon, Cornell Un... 3 127 7.90E-06 1
G1820 AW000203 AW000203 614009A09.y1 614 - root cDNA librar... 1 134 8.00E-06 1
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CD
Figure imgf000104_0003
O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O CNI C CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM oo oo oo oo oo oo co oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ δδδδδ mmmmmmmmmm m mmmmm mmmmmmmm mmmmmm Table 2
G1820 GI-15528742 hypothetical protein [Oryza sativa] -1 54 0.9995
G1820 GI-1087017 arabinogalactan-protein, AGP [Nicotiana al... 2 76 0.99994
G1820 GI-7489085 T10265 arabinogalactan-protein AGP2 - Pers... 2 76 0.99994
G1820 GI-2305113 metallothionein-like protein 3 53 0.99995
G1820 GI-15624001 hypothetical protein [Oryza sativa] -2 53 0.99995
G2010 BI931517 BI931517 EST551406 tomato flower, 8 mm to p... 3 232 2.60E-31 2
G2010 BI928213 BI928213 EST548102 tomato flower, 3 - 8 mm ... 2 232 2.70E-31 2
G2010 BG525285 BG525285 48-3 Stevia field grown leaf cDNA ... 2 222 3.20E-31 2
G2010 BI929558 BI929558 EST549447 tomato flower, 3 - 8 mm ... 1 228 1.00E-30 2
G2010 AW096676 AW096676 EST289856 tomato mixed elicitor, B... 2 232 1.20E-30 2
G2010 BE435668 BE435668 EST406746 tomato breaker fruit, TI... 3 216 7.80E-30 2
G2010 BE058432 BE058432 sn16a06.y1 Gm-c1016 Glycine max cD... 1 186 1.30E-29 2
G2010 AW933950 AW933950 EST359793 tomato fruit mature gree... 3 216 1.60E-29 2
G2010 AMSPB1 X92369 A.majus mRNA for squamosa-promoter b... 2 212 1.60E-29 2
G2010 BG455868 BG455868 NF068F05PL1 F1045 Phosphate starved... 1 219 3.10E-29 2
G2010 BG524211 BG524211 39-94 Stevia field grown leaf cDNA... 1 200 7.50E-29 2
G2010 BI071295 BI071295 C054P79U Populus strain T89 leaves... 1 201 6.20E-28 2
G2010 BM732448 BM732448 sal77d10.y1 Gm-c1061 Glycine max c... 1 200 6.90E-28 2
G2010 BE499739 BE499739 WHE0975_G04_M07ZS Wheat pre-anthes... 1 199 6.90E-27 2
G2010 BE060783 BE060783 HVSMEg0013F15f Hordeum vulgare pre... 3 195 9.80E-27 2
G2010 BG097153 BG097153 EST461672 potato leaves and petiol... 3 187 3.10E-25 2
G2010 BF626329 BF626329 HVSMEa0018F24f Hordeum vulgare see... 1 175 4.10E-25 2
G2010 AMSBP2 X92079 A.majus mRNA for squamosa-promoter b... 2 169 4.30E-25 2
G2010 BF650908 BF650908 NF098D09EC1 F1076 Elicited cell cul... 1 179 1.20E-24 2
G2010 AW747167 AW747167 WS1_66_F11.b1_A002 Water-stressed ... 1 185 1.30E-24 2
G2010 BM140427 BM140427 WHE0483_c08_e15zS Wheat Fusarium g... 1 174 1.50E-24 2
G2010 BF634536 BF634536 NF060C08DT1 F1065 Drought Medicago ... 1 181 5.20E-24 2
G2010 AMA011622 AJ011622 Antirrhinum majus mRNA for squamos... 3 180 8.50E-24 2
G2010 BI930428 BI930428 EST550317 tomato flower, 8 mm to p... 2 174 8.60E-24 2
G2010 BF482644 BF482644 WHE2301-2304_A21_A21ZS Wheat pre-a... 2 178 9.70E-24 2
G2010 BF202242 BF202242 WHE0984_D01_G02ZS Wheat pre-anthes... 2 178 1.00E-23 2
G2010 RICRMSOA L34039 Oryza sativa manganese superoxide di... -1 187 1.20E-23 2
G2010 BG123992 BG 123992 EST469638 tomato shoot/meristem Ly... 3 183 2.10E-23 2
G2010 BG789832 BG789832 sae56b07.y1 Gm-c1051 Glycine max c... 2 170 2.30E-23 2
G2010 BG595750 BG595750 EST494428 cSTS Solanum tuberosum c... 3 179 2.50E-23 2
G2010 AW691786 AW691786 NF044B06ST1 F1000 Developing stem M... 3 168 4.80E-23 2
CM CNI CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM
Figure imgf000106_0001
co ι-. rt r- r-- oo r-- co co co cD co cΛ co r-. r-. cΛ CD σ) rt m cM i-- r-- m cM co rt cθ ι- oo ^- oθ τ- τ- ^- ι-- oo ι-- oo oo oo oo co cD m oo r-. f-. co co oo co r-. co r^ r-- oo co co oo m m m f-- m r-- oo co r-.
M - -r- O ^ ^ ^ τ- -r- -ι- CO CM ^ CO O θ M CM ι- C CM 00 CM c ) CM CO - -r- CM 't- M
CN
Figure imgf000106_0002
sllgiSSiSgsslsgiSlllllsiisgililSigsii
2? S 2 ^ § Lu S 22 Lu 2 Lu l 20 L? 5 i i = w ^ i o § l § 25 o t S S w co m < < < ω m m m m ω N CQ < < < N < N N co O O co cD < N < < < ι cQ co co O oooooooooooooooooooooooooooooooooooo o CMoCMoCM CoMoCM CoMoCMoCM CoMoCMoCMoCMoCMoCMoCM CoM CoMoCMoCMoCM CoMoCMoCMoCM CoM CoMoM CoMoCMσCMoCMoCλioM coloo
O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O
r-~ r- — r — r — r — r — i — i — r— • r — r — i — r-— r— - i — i — r-— ι-— r-— i — i- — ι-— i — i — r — — ι-~- r-— r~- r— - ' — r — r-— •--- r~- i — Table 2
G2010 BF096268 BF096268 EST360317 tomato nutrient deficien... 1 154 1.70E-18 2
G2010 AP004115 AP004115 Oryza sativa chromosome 2 clone OJ... -1 175 1.90E-18 2
G2010 AP004324 AP004324 Oryza sativa chromosome 6 clone OJ... -2 176 3.60E-18 2
G2010 AC 108762 AC108762 Oryza sativa chromosome 9 clone OS... -2 158 5.30E-18 2
G2010 BH659801 BH659801 BOMFC96TF BO_2_3_KB Brassica olera... 2 230 5.60E-17 1
G2010 BG582086 BG582086 EST483824 GVN Medicago truncatula ... 1 144 3.30E-16 2
G2010 BE057780 BE057780 sn07b01.y1 Gm-d 016 Glycine max cD... 3 224 5.10E-16 1
G2010 AW091803 AW091803 EST284983 tomato mixed elicitor, B... 2 223 6.70E-16 1
G2010 BM536411 BM536411 EST589433 tomato breaker fruit Lye... 3 220 7.00E-16 1
G2010 BE805023 BE805023 ss35d09.y1 Gm-c1061 Glycine max cD... 1 121 8.50E-16 2
G2010 AW929797 AW929797 EST354067 tomato flower buds 8 mm ... 1 220 1.00E-15 1
G2010 BI436257 BI436257 EST539018 cSTE Solanum tuberosum c... 3 218 1.10E-15 1
G2010 AW929795 AW929795 EST354065 tomato flower buds 8 mm ... 1 220 1.20E-15 1
G2010 BI919686 BI919686 EST539621 potato microtubers, in v... 3 218 1.40E-15 1
G2010 AW694881 AW694881 NF081A09ST1 F1068 Developing stem M... 1 135 2.50E-15 2
G2010 BE434312 BE434312 EST405390 tomato breaker fruit, TI... 1 205 3.10E-14 1
G2010 BG451969 BG451969 NF043G05DT1 F1037 Drought Medicago ... 3 135 7.60E-14 2
G2010 AW690194 AW690194 NF029F09ST1 F1000 Developing stem M... 3 135 7.90E-14 2
G2010 BM374244 BM374244 EBpi03_SQ003_B07_R IGF Barley EBpi... 3 199 2.60E-13 1
G2010 AW982253 AW982253 HVSMEg0002l10f Hordeum vulgare pre... 2 195 2.80E-13 1
G2010 BH693623 BH693623 BOMPV21TR BO_2_3_KB Brassica olera... 3 194 3.50E-13 1
G2010 BG452315 BG452315 NF083G06LF1 F1052 Developing leaf M... 2 135 4.60E-13 2
G2010 AU088772 AU088772 AU088772 Lotus japonicus flower bu... 2 193 4.90E-13 1
G2010 AI657269 AI657269 486092G09.y1 486 - leaf primordia ... 1 185 5.00E-12 1
G2010 BE460553 BE460553 EST411972 tomato breaker fruit, TI... 3 113 6.70E-12 2
G2010 AMA011621 AJ011621 Antirrhinum majus mRNA for squamos... 1 184 1.30E-11 1
G2010 AC092557 AC092557 Oryza sativa chromosome 3 clone OS... 2 187 1.60E-11 1
G2010 AP004744 AP004744 Oryza sativa chromosome 6 clone OS... 1 135 1.80E-11 2
G2010 AI820134 AI820134605086D06.X3 605 - Endosperm cDNA ... -3 1 9 2.80E-11 1
G2010 AW222771 AW222771 EST299582 tomato fruit red ripe, T... 2 176 4.20E-11 1
G2010 BI307968 BI307968 EST529378 GPOD Medicago truncatula... 3 174 6.00E-11 1
G2010 BM359177 BM359177 GA Ea0017G06r Gossypium arboreum ... 2 173 6.70E-11 1
G2010 BG442540 BG442540 GA Ea0017G06f Gossypium arboreum ... 2 173 8.20E-11 1
G2010 ZMU89496 U89496 Zea mays ligulelessl protein (ligule... 3 176 1.20E-10 1
G2010 BG933421 BG933421 WS1_2_B01.b1_A002 Water-stressed 1... 2 173 1.20E-10 1
G2010 Bl 128093 BI128093 G070P72Y Populus cambium cDNA libr... 3 168 3.80E-10 1
Table 2
7 G2010 BH742595 BH742595 gu77e09.g1 BoBudsOI Brassica olera... 3 164 8.10E-10
7 G2010 AC108759 AC108759 Oryza sativa chromosome 9 clone OS... -1 170 1.00E-09
7 G2010 BG440913 BG440913 GA Ea0010P04f Gossypium arboreum ... 1 161 1.40E-09
7 G2010 BE806499 BE806499 ss59f10.y1 Gm-c1062 Glycine max cD... 3 161 2.20E-09
7 G2010 BF704779 BF704779 FM1_51_D08.b1_A003 Floral-Induced ... 3 160 2.70E-09
7 G2010 BI933324 BI933324 EST553213 tomato flower, anthesis ... 3 157 3.30E-09
7 G2010 AP002482 AP002482 Oryza sativa genomic DNA, chromoso... 3 164 4.40E-09
7 G2010 L38193 L38193 BNAF1025E Mustard flower buds Brassi... 1 158 4.70E-09
7 G2010 BE518391 BE518391 WHE0815_F09_L17ZS Wheat vernalized... 2 100 5.50E-09
7 G2010 AW982980 AW982980 HVSMEg0004L14f Hordeum vulgare pre... 3 157 7.70E-09
7 G2010 BH580426 BH580426 BOGOB55TF BOGO Brassica oleracea g... -2 157 7.90E-09
7 G2010 ZMA011620 AJ011620 Zea mays mRNA for SBP-domain prote... 2 155 1.00E-08
7 G2010 BE023083 BE023083 sm90e08.y1 Gm-c1015 Glycine max cD... 1 152 1.80E-08
7 G2010 BG630869 BG630869 cC-esflcLEL5D24d1 Tomato flower Ii... -1 150 3.00E-08
7 G2010 BI927983 BI927983 EST547860 tomato flower, 3 - 8 mm ... 1 149 3.10E-08
7 G2010 AU091662 AU091662 AU091662 Rice panicle at flowering... 2 150 3.20E-08
7 G2010 BI928266 BI928266 EST548155 tomato flower, 3 - 8 mm ... 1 148 3.30E-08
7 G2010 BI927982 B 1927982 EST547859 tomato flower, 3 - 8 mm ... 1 148 3.60E-08
©
-4 7 G2010 AV835022 AV835022 AV835022 K. Sato unpublished cDNA ... 3 147 5.60E-08
7 G2010 BH408621 BH408621 1007005H05.1EL_x1 1007 - RescueMu ... 2 147 6.50E-08
7 G2010 BE804992 BE804992 ss34h10.y1 Gm-c1061 Glycine max cD... 2 143 1.70E-07
7 G2010 BG791002 BG791002 sae73a09.y1 Gm-c1064 Glycine max c... -3 135 1.40E-06
7 G2010 BF422188 BF422188 FM1_12_H07.b1_A003 Floral-Induced ... 1 132 2.00E-06
7 G2010 BVCOXII X55297 B.vulgaris coxll gene for cytochrome... 2 143 4.10E-06
7 G2010 C72011 C72011 C72011 Rice panicle at flowering sta... 1 131 4.20E-06
7 G2010 AP000396S2 AP000397 Beta vulgaris mitochondrial genomi... 2 143 5.20E-06
7 G2010 BH481141 BH481141 BOGXV25TR BOGX Brassica oleracea g... -1 140 5.20E-06
7 G2010 AB008450 AB008450 Beta vulgaris mitochondrial rps7 g... 2 142 6.50E-06
7 G2010 BG 129294 BG 129294 EST474940 tomato shoot/meristem Ly... 3 129 9.30E-06
7 G2010 BF596468 BF596468 su51f06.y1 Gm-c1069 Glycine max cD... 1 130 3.90E-05
7 G2010 BG447166 BG447166 GA Eb0040N14f Gossypium arboreum ... 2 130 4.90E-05
7 G2010 BE400548 BE400548 AWB004.H05F000328 ITEC AWB Wheat M... 1 121 5.50E-05
Figure imgf000108_0001
7 G2010 BF485120 BF485120 WHE1793_F08_L15ZS Wheat pre-anthes... 2 124 6.40E-05
7 G2010 BI470983 BI470983 sah92a05.y1 Gm-c1050 Glycine max c... 3 119 6.60E-05
7 G2010 C72359 C72359 C72359 Rice panicle at flowering sta... 2 120 7.10E-05
7 G2010 ZMA011619 AJ011619 Zea mays mRNA for SBP-domain prote... 3 132 0.00013
Table 2
7 G2010 AV395862 AV395862 AV395862 Chlamydomonas reinhardtii... 3 114 0.00027
7 G2010 BG557285 BG557285 EM1_42_A05.b1_A002 Embryo 1 (EM1) ... 1 116 0.00029
7 G2010 AW509094 AW509094 sh30a06.y1 Gm-c1017 Glycine ma cD... 2 114 0.0003
7 G2010 AQ289632 AQ289632 nbxb0019K20f CUGI Rice BAC Library... 2 123 0.0014
7 G2010 BI925725 BI925725 EST545614 tomato flower, buds 0-3 ... 1 121 0.0014
7 G2010 BH230073 BH230073 1006155H05.x1 1006 - RescueMu Grid... -1 108 0.0014
7 G2010 AU083475 AU083475 AU083475 Rice panicle (longer than... 2 115 0.0022
7 G2010 AV396403 AV396403 AV396403 Chlamydomonas reinhardtii... 2 114 0.0022
7 G2010 BG651519 BG651519 sad47h02.y1 Gm-c1075 Glycine max c... 3 113 0.0023
7 G2010 BF587332 BF587332 FM1_35_B04.b1_A003 Floral-Induced ... 3 106 0.0024
7 G2010 BE499423 BE499423 WHE0974_F04_L08ZS Wheat pre-anthes... 2 116 0.0025
7 G2010 AW944940 AW944940 EST336990 tomato flower buds 3-8 m... 2 107 0.0041
7 G2010 AI726550 AI726550 BNLGHJ6118 Six-day Cotton fiber Go... 2 118 0.0049
7 G2010 BH689424 BH689424 BOMPQ46TR BO_2_3_KB Brassica olera... -1 118 0.0053
7 G2010 AV393371 AV393371 AV393371 Chlamydomonas reinhardtii... 3 114 0.0054
7 G2010 AI813122 AI813122 23C10 Pine Lambda Zap Xylem librar... 2 108 0.0054
7 G2010 BG947454 BG947454 IP1_4_F04.b1_A002 Immature pannicl... 3 115 0.0075
7 G2010 BG947455 BG947455 IP1_4_F05.b1_A002 Immature pannicl... 3 115 0.0075
7 G2010 BF483589 BF483589 WHE2335_E11_J21ZS Wheat pre-anthes... 1 115 0.0075
7 G2010 BG320129 BG320129 Zm03_05c03_A Zm03_AAFC_ECORC_cold_... -2 114 0.017
7 G2010 C91611 C91611 C91611 Rice panicle (longer than 10c... 3 97 0.03
7 G2010 BG594131 BG594131 EST492809 cSTS Solanum tuberosum c... 1 109 0.054
7 G2010 BM111172 BM111172 EST558708 potato roots Solanum tub... 2 109 0.063
7 G2010 BG124241 BG124241 EST469875 tomato shoot meristem Ly... 2 109 0.064
7 G2010 BH229772 BH229772 1006154C12.x1 1006 - RescueMu Grid... -1 94 0.087
G2010 BF617209 BF617209 HVSMEc0015A05f Hordeum vulgare see... 1 107 0.12
G2010 AV630386 AV630386 AV630386 Chlamydomonas reinhardtii... 3 92 0.27
7 G2010 BE607271 BE607271 NXCI_034_H03_F NXCI (Nsf Xylem Com... 1 89 0.28
7 G2010 BE411990 BE411990 JJL001.D04R990511 ITEC JJL Wheat L... 3 99 0.66
7 G2010 BM535038 BM535038 EST588060 tomato breaker fruit Lye... 3 89 0.96
7 G2010 AQ936495 AQ936495 Gm_UMb001_106_F10F UMN Soybean BAC... -1 80 0.97
7 G2010 BI9594 8 BI959418 HVSMEn0019J11f Hordeum vulgare rac... 2 92 0.998
7 G2010 BH475782 BH475782 BOHPK53TF BOHP Brassica oleracea g... 3 91 0.99995
7 G2010 GI-1183866 squamosa-promoter binding protein 1 -1 212 9.90E-32 2
7 G2010 GI-2130003 S62360 squamosa-promoter binding protein 1... -1 212 9.90E-32 2
7 G2010 GI-6094239 SBP1_ANTMA SQUAMOSA-PROMOTER BINDING PROTE. -1 212 9.90E-32 2
Table 2
G2010 GI-1183864 squamosa-promoter binding protein 2 169 9.40E-28 2
G2010 GI-2130004 S62361 squamosa-promoter binding protein 2... 169 9.40E-28 2
G2010 GI-6094241 SBP2_ANTMA SQUAMOSA-PROMOTER BINDING PROTE. 169 9.40E-28 2
G2010 GI-5931641 squamosa promoter binding protein-homologue 4 180 8.20E-27 2
G2010 GI-5931643 squamosa promoter binding protein-homologue 5 174 2.30E-25 2
G2010 GI-5931780 SBP-domain protein 2 175 5.50E-25 3
G2010 GI-5931778 SBP-domain protein 1 179 2.10E-23 2
G2010 GI-5931784 SBP-domain protein 4 176 6.10E-23 2
G2010 GI-5931782 SBP-domain protein 3 174 1.10E-22 2
G2010 GI-5931786 SBP-domain protein 5 154 6.00E-22 2
G2010 GI-8468036 Similar to Arabidopsis thaliana chromosome... 164 1.60E-20 2
G2010 GI-5931639 squamosa promoter binding protein-homologue 3 184 5.50E-14
G2010 GI-1914845 ligulelessl protein 176 1.10E-12
G2010 GI-6016502 LG1_MAIZE LIGULELESS1 PROTEIN 176 1.10E-12
G2010 GI-7489786 T04328 ligulelessl protein - maize 176 1.10E-12
G2010 GI-17940734 AF451895_1 LIGULELESS1 [Zea mays] 176 1.10E-12
G2010 GI-5931790 SBP-domain protein 7 155 3.90E-11
G2010 GI-9087308 orf102a [Beta vulgaris var. altissima] 143 7.40E-10
G2010 GI-9838446 orf102a [Beta vulgaris var. altissima] 143 7.40E-10
G2010 GI-5931788 SBP-domain protein 6 132 1.30E-06
G2010 GI-18461278 hypothetical protein [Oryza sativa] 56 0.99
G1037 BH007675 BH007675 ee72e06.x1 TO1000 Brassica oleracea... 2 830 1.60E-80
G1037 BM407041 BM407041 EST581368 potato roots Solanum tube... 3 363 1.80E-65 4
G1037 AB062095 AB062095 Zea mays ZmRR9 mRNA for response re... 3 339 1.00E-61 4
G1037 AP004094 AP004094 Oryza sativa chromosome 2 clone OJ1... -1 334 4.10E-58 4
G1037 AP003975 AP003975 Oryza sativa chromosome 2 clone OJ1... -3 334 6.40E-58 4
G1037 AP004087 AP004087 Oryza sativa chromosome 2 clone OJ1... 2 374 3.90E-57 4
G1037 AP004552 AP004552 Oryza sativa chromosome 6 clone OJ1... 1 370 1.70E-55 3
G1037 BG450692 BG450692 NF102C07DT1 F1052 Drought Medicago t... 3 396 1.00E-51 2
G1037 AB071695 AB071695 Zea mays ZmRR10 mRNA for response r... 1 313 7.30E-51 4
G1037 AB060130 AB060130 Zea mays ZmRR8 mRNA for response re... 1 308 4.50E-50 4
G1037 AC107226 AC107226 Oryza sativa chromosome 3 clone OSJ... 1 321 9.30E-49 4
G1037 BM336723 BM336723 MEST198-A02.T3 ISUM5-RN Zea mays cD... -2 345 2.40E-47 2
G1037 BE205320 BE205320 EST397996 KVO Medicago truncatula c... 2 220 1.40E-46 3
G1037 AL372190 AL372190 MtBA49C02F1 MtBA Medicago truncatul... 1 220 2.40E-44 3
G1037 AW564570 AW564570 LG1_296_E01.b1_A002 Light Grown 1 (... 2 210 4.30E-42 3
CM CM C CM CO CM CO W CM n oO CO CM CM CO OO CO CM CM CO CM OO CM CM CM CM CM CM CM CM CM CM CM CM CM CM
O OO OO CO r- LO -O rf rf rt rf rf C CO CO CM CM τ- O O O CΛ Cn cO Cθ r-- LO LO LO rt CO CM CM !- rt rt rt co co co co co co co cO co co co CO CO CO C CO CO CO CO CO CM CM J CM CM CM C CM CM CM CM CM CM
LU LU UI LU LU lU LU UJ LU LU UJ LU LU LU LlJ LU LU LU o o o o o o o o o o o o o o o o O O O o o o O o o o m co I--. o ιo oo m CΛ - i- CO CO rt n O T- S ffl 1Λ CO CM O rf 00 o >- rt cn co ι- cD cΛ f- co co τ- τ- cM rt cθ '- rt oo co co cM ι- r-. oo τ- m '- rt l rt L co T- m i- r-- co
C0 O CM CM rt l-- C0 rt C0 rf r^ C0 O cn ι- rf rt c0 O CΛ O rf C0 l-- C0 CM CM rf ι- 7- cn 00 rf 00 - τ- . oθ τ- cM cn rf oo cn cM cn cΛ rf co rf oo m m cn cM oo - cΛ CΛ θ θ CΛ co o co oo oo oo oo o r-. co
CM CO CM CO - CO T- T- CO '- I- CM CM CM ^- CM CM CM CM ^- CM T- - CM CM I- CM CM I- ^- '^- T- T- CM -'- 'I-
CM C T- ^ M CO T- CO CO CO ^ CM C CO CM T- CO CM C ^ CO C O CO ^ ^ C CM CO C CO CM
Figure imgf000111_0001
fc ^ ^ ^ !i t t |^ ^ ,^ ^ ι^ ι^ ^ ι^ ι^ ι^ ι^ ,^ f-- '-- ι-- ι^ r-. r-- r-- r-- r-- r^ co co co co co co c co co co crj co co co co co co co co co co co co co co co co co o co co co co co co co o σ o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o oδ δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ
CΛ CΛ CΛ CΛ CΛ CΛ CΛ CΛ Cn cΛ CΛ CΛ CΛ CD σj CΛ CΛ CΛ CΛ Cn Cn cΛ CΛ Cn CΛ Cn cjJ CΛ Cn CΛ CΛ CΛ CΛ CΛ CΛ Cn Table 2
G1037 BM086086 BM086086 sah35c12.y1 Gm-c1036 Glycine max cD... 3 177 9.70E-21 2
G1037 BM736413 BM736413 952050D06.X1 952 - BMS tissue from ... -1 161 2.90E-20 2
G1037 BG560474 BG560474 RHIZ2_74_H01.b1_A003 Rhizome2 (RHIZ... 2 194 4.80E-20 2
G1037 BE597633 BE597633 PI1_72_B12.b1_A002 Pathogen induced... 1 194 4.90E-20 2
G1037 C74559 C74559 C74559 Rice panicle shorter than 3cm ... 3 180 5.10E-19 2
G1037 BH592456 BH592456 BOGDT29TR BOGD Brassica oleracea ge... -3 170 1.10E-18 2
G1037 AC027038 AC027038 Oryza sativa chromosome 10 clone OS... -1 197 2.80E-18 3
G1037 BM736421 BM736421 952050E03.x1 952 - BMS tissue from ... 1 151 7.60E-17 2
G1037 A2135727 AZ135727 Gm_UMb001_190_P19R UMN Soybean BAC ... -2 237 2.60E-16
G1037 BG592527 BG592527 EST491205 cSTS Solanum tuberosum cD... 2 189 2.10E-11
G1037 AL381868 AL381868 MtBC03E05F1 MtBC Medicago truncatul... 3 181 3.20E-10
G1037 AL367833 AL367833 MtBA19F01 F1 MtBA Medicago truncatul... 1 172 4.00E-09
G1037 BG 123738 BG123738 EST469384 tomato shoot/meristem Lye... 1 166 2.30E-08
G1037 BM325142 BM325142 PIC1_40_B10.b1_A002 Pathogen-infect... 3 161 2.40E-08
G1037 BF068683 BF068683 st31d08.y1 Gm-c1067 Glycine max cDN... 2 161 3.10E-08
G1037 AU084218 AU084218 AU084218 Cryptomeria japonica inner... 1 161 3.10E-08
G1037 BE559431 BE559431 HV_CEb0024K14f Hordeum vulgare seed... 2 108 4.60E-08
G1037 BH477453 BH477453 BOGXM90TR BOGX Brassica oleracea ge... 3 151 3.00E-07
G1037 AW719680 AW719680 LjNEST8C3r Lotus japonicus nodule I... 1 151 3.40E-07
G1037 BM520832 BM520832 sal31 a11.y1 Gm-d 059 Glycine max cD... 2 149 5.30E-07
G1037 AV932456 AV932456 AV932456 K. Sato unpublished cDNA I... 1 102 1.70E-06
G1037 BH728943 BH728943 BOMEE94TF BO_2_3_KB Brassica olerac... -3 141 4.40E-06
G1037 BM498464 BM498464 952026F12.x1 952 - BMS tissue from ... 2 135 1.70E-05
G1037 AW944749 AW944749 EST336799 tomato flower buds 3-8 mm... 1 140 1.80E-05
G1037 AG024105 AG024105 Oryza sativa DNA, 3' flanking seque... -2 136 2.30E-05
G1037 BE606498 BE606498 WHE0908_C07_F14ZS Wheat 5-15 DAP sp... 2 134 2.40E-05
G1037 BM326134 BM326134 PIC1_66_B06.b1_A002 Pathogen-infect... 2 133 2.90E-05
G1037 BH575345 BH575345 BOHIU91TF BOHI Brassica oleracea ge... -3 142 3.20E-05
G1037 BH538825 BH538825 BOGKD72TF BOGK Brassica oleracea ge... 3 146 6.00E-05
G1037 BH633001 BH633001 1007058A08.y1 1007 - RescueMu Grid ... 1 130 0.00013
G1037 BE353296 BE353296 EST400428 L. hirsutum trichome, Cor... 3 127 0.00013
G1037 BLYDO L44046 Hordeum vulgare (clone ABG603) chromo... -2 138 0.00014
G1037 BM134876 BM134876 WHE0452_H01_H01ZS Wheat Fusarium gr... 2 132 0.00017
G1037 BH604770 BH604770 BOHSC62TF BOHS Brassica oleracea ge... 1 122 0.00059
G1037 BE660997 BE660997 312 GmaxSC Glycine max cDNA, mRNA s... 1 121 0.00061
G1037 BH744755 BH744755 gt41f02.b1 BoBudsOI Brassica olerac... 2 120 0.00077
Table 2
G1037 AL369717 AL369717 MtBA32H05F1 MtBA Medicago truncatul... 1 116 0.0022 1
G1037 AW694254 AW694254 NF074C08ST1 F1065 Developing stem Me... 1 129 0.0024 1
G1037 BI469444 BI469444 sai12a08.y1 Gm-c1053 Glycine max cD... 3 115 0.0029 1
G1037 AP003831 AP003831 Oryza sativa chromosome 7 clone OJ1... -1 107 0.003 2
G1037 BI405862 BI405862 147F09 Mature tuber lambda ZAP Sola... 2 119 0.0032 1
G1037 BI948917 BI948917 HVSMEI0011 H03f Hordeum vulgare spik... 2 121 0.0045 1
G1037 BI074783 BI074783 IP1_15_B09.b1_A002 Immature pannicl... 3 117 0.0048 1
G1037 AW689536 AW689536 NF021 H03ST1 F1000 Developing stem Me... 2 126 0.0057 1
G1037 BM325391 BM325391 PIC1_44_A04.b1_A002 Pathogen-infect... 1 117 0.0079 1
G1037 BM187690 BM187690 saj87g12.y1 Gm-c1074 Glycine max cD... 2 120 0.01 1
G1037 BM736452 BM736452 952051 A04.x1 952 - BMS tissue from ... -3 112 0.01 1
G1037 BF070582 BF070582 st22e02.y1 Gm-c1065 Glycine max cDN... -3 123 0.011 1
G1037 BG 133615 BG133615 EST466423 tomato crown gall Lycoper... 2 109 0.013 1
G1037 AW218302 AW218302 EST303483 tomato radicle, 5 d post-... 2 110 0.013 1
G1037 BG644799 BG644799 EST506418 KV3 Medicago truncatula c... 2 111 0.017 1
G1037 BG522035 BG522035 17-58 Stevia field grown leaf cDNA ... 2 120 0.017 1
G1037 AW650154 AW650154 EST328608 tomato germinating seedli... 3 109 0.023 1
G1037 AV936280 AV936280 AV936280 K. Sato unpublished cDNA I... -1 120 0.028 1
G1037 BI781163 BI781163 EBma03_SQ001_K03_R IGF Barley EBmaO... 1 108 0.03 1
G1037 AV936377 AV936377 AV936377 K. Sato unpublished cDNA I... -3 120 0.033 1
G1037 AV937037 AV937037 AV937037 K. Sato unpublished cDNA I... -1 120 0.033 1
G1037 AV936494 AV936494 AV936494 K. Sato unpublished cDNA I... -1 120 0.034 1
G1037 BH693119 BH693119 BOHTJ77TR BO_2_3_KB Brassica olerac... -1 118 0.04 1
G1037 AI054482 AI054482 coau0001B23 Cotton Boll Abscission ... 3 119 0.04 1
G1037 BH417518 BH417518 1007058A08.2EL_x1 1007 - RescueMu G... 3 106 0.046 1
G1037 BH556049 BH556049 BOGOM92TR BOGO Brassica oleracea ge... -3 118 0.067 1
G1037 BM325806 BM325806 PIC1_53_A05.b1_A002 Pathogen-infect... 1 112 0.072 1
G1037 BG789991 BG789991 sae61f06.y1 Gm-c1064 Glycine max cD... 1 113 0.089 1
G1037 BF008920 BF008920 ss71a03.y1 Gm-c1062 Glycine max cDN... 3 102 0.094 1
G1037 BH432713 BH432713 BOGGB46TF BOGG Brassica oleracea ge... 3 117 0.098 1
G1037 BI272862 BI272862 NF043C09FL1F1069 Developing flower ... 1 116 0.1 1
G1037 BH488734 BH488734 BOHLY95TF BOHL Brassica oleracea ge... -1 115 0.16 1
G1037 AI974630 AI974630 T113082e KV2 Medicago truncatula cD... 2 107 0.22 1
G1037 BM309528 BM309528 sak63f11.y1 Gm-c1036 Glycine max cD... 1 109 0.26 1
G1037 C72732 C72732 C72732 Rice panicle at flowering stag... 1 105 0.28 1
G1037 BF642451 BF642451 NF063G08IN1 F1066 Insect herbivory M... 3 111 0.28 1
n Q g c Lo co r^ M rf rf W rt in i^ cg o Λ CM in iΛ to co to S S SS ϋ fc ϋ lϊ fc S co rf m CO CO CO CO r- r- r-- σ σ) σ C c σ 02) 02) 02) 20) 02) 02) 02) 02) 02) 02)
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Figure imgf000114_0001
m ιo oo _. CM CM !2 _-, Ui W N ,li N j; o S ) j5 t ffl lO n » N « ? « Ol S j l C2 C3 iθ LU D 5 ie L
Figure imgf000114_0002
< C0 CQ CQ CQ < C0 < < CQ CQ r— i— -^ I-— r-- I-~ I-— Γ— -- r— f— r-~ r— r— f~- r— r-~ r— r— r-~ r-— r— i— r— r-- *^— i— — i— i— i— r— r— r— i— r— co co co co co co o co co co co co co o oo co co oo co co co co co oo c co co co co co oo co co co co
O O P P P O O O O O O O O O P P P P P P O O O O O O O O O O O O O O O O δδδoδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ cn cn cn cn o cn cn cΛ cn cn cn cn cΛ cn cΛ CΛ cn cΛ CΛ cn cΛ CΛ CΛ cΛ CΛ CΛ cn p cn cΛ cn cΛ CΛ cn cn cn Table 2
G1037 BG508638 BG508638 sac75e04.y1 Gm-d 072 Glycine max cD... 1 98 0.999 G1037 AV938915 AV938915 AV938915 K. Sato unpublished cDNA I... 2 99 0.999 G1037 BM817109 BM817109 HC02A08_T3.ab1 HC Hordeum vulgare c... 1 101 0.999 G1037 VCVOLOPS Y11204 V.carteri gene encoding volvoxopsin. ... -2 103 0.9996 G1037 BG098164 BG098164 EST462683 sprouting eyes/shoots Sol... 1 97 0.9996 G1037 AW030466 AW030466 EST273721 tomato callus, TAMU Lycop... 3 95 0.9998 G1037 BH671742 BH671742 BOMLT29TF BO_2_3_KB Brassica olerac. 2 95 0.9998 G1037 BH472761 BH472761 BOHKP85TF BOHK Brassica oleracea ge.. -1 90 0.99991 G1037 GI-14189890 response regulator 9 [Zea mays] -1 339 2.20E-64 4 G1037 GI-15667625 response regulator 10 [Zea mays] -1 313 3.80E-56 6 G1037 GI-13661174 response regulator 8 [Zea mays] -1 308 1.30E-54 5 G1037 GI-15289981 hypothetical protein [Oryza sativa] -3 98 5.90E-09 4 G1037 GI-11041692 response regulator [Zea mays] -2 75 0.00016 3 G1037 GI-12060388 response regulator 6 [Zea mays] -1 109 0.0016 2 G1037 GI-12225924 unnamed protein product [Zea mays] -1 109 0.0016 2 G1037 GI-12060390 response regulator 7 [Zea mays] -1 71 0.0046 4 G1037 GI-1076557 S54157 extensin-like protein - cowpea (tra... 2 109 0.0072 1 G1037 GI-13173408 AF339732_1 response regulator protein [Dia... -1 71 0.0076 4 G1037 GI-12225928 unnamed protein product [Zea mays] -1 76 0.0092 3 G1037 GI-12060386 response regulator 5 [Zea mays] -1 72 0.026 3 G1037 GI-18461236 Myb-related transcription factor-like prot... -3 87 0.037 2 G1037 GI-13940498 AF318581_1 putative transcription factor O... -1 106 0.07 2 G1037 GI-13940496 AF318580_1 putative transcription factor Z... -1 100 0.084 2 G1037 GI-5689236 ZmRR2 -2 69 0.15 2 G1037 GI-6009897 response regulator 2 -2 69 0.15 2 G1037 GI-11358972 T50858 repsonse regulator ZmRR2 [imported]... -2 69 0.15 2 G1037 GI-12328569 hypothetical protein [Oryza sativa] -1 71 0.27 3 G1037 GI-13940500 AF318582_1 putative transcription factor O... -3 89 0.32 2 G1037 GI-17227037 AF442139_1 style-specific self-incompatibi... -1 73 0.32 1 G1037 GI-4566516 AF108140_1 gamete-specific homeodomain pro... 2 100 0.37 1 G1037 GI-16209575 gamete-specific homeodomain protein [Chlam... 2 100 0.37 1 G1037 GI-17227039 AF442140_1 style-specific self-incompatibi... -1 70 0.56 1 G1037 GI-14626761 AF395901_1 mature anther-specific protein ... -3 67 0.88 2 G1037 GI-19925 pistil extensin like protein, partial CDS 2 66 0.89 1 G1037 GI-100367 S24619 pistil extensin-like protein - comm... 2 66 0.89 1 G1037 GI-11034707 hypothetical protein-similar to Arabidopsi... -3 89 0.95 1
Table 2
G1037 GI-4519671 transfactor -1 85 0.97 1 G1037 GI-12060384 response regulator 4 [Zea mays] -1 69 0.99 3 G1037 GI-4106378 calcium-binding protein -1 79 0.997 1 G1037 GI-15528802 putative response regulator [Oryza sativa] -1 70 0.9991 2 G1037 GI-11177540 AF298118_1 putative transcription factor G... -1 84 0.9999 1 G1037 GI-13940494 AF318579_1 putative transcription factor G... -1 84 0.9999 1 G1947 AP003826 AP003826 Oryza sativa chromosome 7 clone OJ1... -3 284 6.80E-45 2 G1947 AC027658 AC027658 Oryza sativa subsp. japonica BAC nb... -2 276 5.20E-44 2 G1947 BE319312 BE319312 NF015D08NR1F1035 Nodulated root Med... 1 283 1.20E-43 2 G1947 BI406849 BI406849 182A06 Mature tuber lambda ZAP Sola... 2 265 3.90E-43 3 G1947 AC092076 AC092076 Oryza sativa chromosome 3 clone OSJ... 2 270 7.50E-43 2 G1947 LPHSF30 X67601 L.peruvianum Lp-hsf30 mRNA for heat s... 3 246 4.20E-42 2 G1947 BM086093 BM086093 sah35d07.y1 Gm-c1036 Glycine max cD... 1 250 8.60E-42 2 G1947 AV833112 AV833112 AV833112 K. Sato unpublished cDNA I... 3 248 1.50E-41 2 G1947 AW036683 A 036683 EST278726 tomato fruit mature green... 1 254 2.30E-41 3 G1947 BG890352 BG890352 EST516203 cSTD Solanum tuberosum cD... 1 264 9.30E-41 3 G1947 BG887370 BG887370 EST513221 cSTD Solanum tuberosum cD... 1 264 2.60E-40 3 G1947 BG886969 BG886969 EST512820 cSTD Solanum tuberosum cD... 3 251 2.70E-40 3 G1947 AW222011 AW222011 EST298822 tomato fruit red ripe, TA... 3 254 2.90E-40 3 G1947 AC105729 AC105729 Oryza sativa chromosome 3 clone OJ1... 3 259 6.40E-40 2 G1947 BF635083 BF635083 NF080A06DT1 F1040 Drought Medicago t... 3 247 2.60E-39 2 G1947 BM521654 BM521654 sak60e12.y1 Gm-c1036 Glycine max cD... 2 267 1.80E-38 2 G1947 AW034874 AW034874 EST279103 tomato callus, TAMU Lycop... 2 229 2.80E-38 2 G1947 AF208544 AF208544 Lycopersicon peruvianum heat stress... 1 253 3.30E-38 2 G1947 BG890138 BG890138 EST515989 cSTD Solanum tuberosum cD... 2 218 7.80E-37 2 G1947 BF459947 BF459947068G10 Mature tuber lambda ZAP Sola... 3 218 1.50E-36 2 G1947 BG643740 BG643740 EST511934 tomato shoot/meristem Lye... 1 218 1.60E-36 2 G1947 BI922160 BI922160 EST542063 tomato callus Lycopersico... 1 218 2.90E-36 2 G1947 BI921583 BI921583 EST541486 tomato callus Lycopersico... 2 218 3.50E-36 2 G1947 AW041695 AW041695 EST284559 tomato mixed elicitor, BT... 1 218 3.50E-36 2 G1947 BG594178 BG594178 EST492856 cSTS Solanum tuberosum cD... 3 218 4.10E-36 2 G1947 BI305378 BI305378 NRS_2_7_8_A01_K18 Drought stress (r... 1 220 4.80E-36 2 G1947 BI305213 BI305213 NRS2-7_8_K18 Drought stress (root) ... 2 224 1.50E-35 2 G1947 GMHSF21 Z46952 G.max mRNA for heat shock transcripti... 1 234 3.20E-35 2 G1947 BG886998 BG886998 EST512849 cSTD Solanum tuberosum cD... 1 218 4.40E-35 2 G1947 BG595575 BG595575 EST494253 cSTS Solanum tuberosum cD... 3 218 7.20E-35 2
CM CM CM CM CM CO CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM
rt rt rf rf rf rt rf rt CO CO CO CM CM CM CM CM CM CM - !- ^ -- ,- -,- ι- τ- ,- τ- -r- !- 0 0 0 0 0 0 CO CO OO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO
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O O rf rf CO 00 rf CΛ CO CM o- oCO rs. cn co cp r-. T- CM rf co r-. co m m co rf co m cn rf in CD 00 CO CO rt CM CM rf LO LO T^ i-^ T^ T-: CM CM M co r-^ CΛ - CM CM CM CO 00
Cn 00 CΛ CM CM C0 O O rt 00 O rt m C0 O O C0 CΛ 00 CΛ 00 CM O 00 CΛ CD r-- CM 00 rt [-» CM rf τ- l-- τ- OO ι- m cθ CD I-- CO CO ^- τ- CO CD CO rf cO rt rt OO - rt ι- CM rt o CM rt cO CO τ- m rf m m rt CΛ CO C CM CM CM CM τ- CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM '1- CM
Figure imgf000117_0001
δδδδδδδδδδδδδδδδδδ δδδδδδδδδδδδδδ Table 2
G1947 AC087771 AC087771 Genomic Sequence for Medicago trunc... -3 244 1.60E-29 2 G1947 BG887112 BG887112 EST512963 cSTD Solanum tuberosum cD... 2 251 1.60E-29 2 G1947 AY052627 AY052627 Phaseolus acutifolius heat shock tr... 1 220 1.70E-29 2 G1947 AL368633 AL368633 MtBA25F11 F1 MtBA Medicago truncatul... 3 241 2.00E-29 2 G1947 AW030725 AW030725 EST273980 tomato callus, TAMU Lycop... 3 218 2.00E-29 2 G1947 Bl138694 BI138694 F113P68Y Populus flower cDNA librar... 1 222 2.90E-29 2 G1947 BG888694 BG888694 EST514545 cSTD Solanum tuberosum cD... 2 189 3.40E-29 2 G1947 BG591987 BG591987 EST499829 P. infestans-challenged I... 2 208 3.60E-29 2 G1947 PSA010643 AJ010644 Pisum sativum hsfA gene, exons 1 to... 1 262 5.60E-29 2 G1947 BG352891 BG352891 sab92f08.y1 Gm-d 040 Glycine max cD... 3 229 6.30E-29 2 G1947 BG890899 BG890899 EST516750 cSTD Solanum tuberosum cD... 2 215 8.00E-29 2 G1947 AC092558 AC092558 Oryza sativa chromosome 3 clone OSJ... -3 247 9.30E-29 2 G1947 C95479 C95479 C95479 Citrus unshiu Miyagawa-wase ma... 1 215 1.10E-28 2 G1947 BF459641 BF459641 062F02 Mature tuber lambda ZAP Sola... 3 183 1.20E-28 2 G1947 BF153546 BF153546 028C10 Mature tuber lambda ZAP Sola... 1 183 1.20E-28 2 G1947 BI135881 BI135881 F059P71Y Populus flower cDNA librar... 3 217 1.70E-28 2 G1947 BM322601 BM322601 PIC1_6_H03.b1_A002 Pathogen-infecte... 1 180 3.30E-28 2 G1947 BG595042 BG595042 EST493720 cSTS Solanum tuberosum cD... 1 181 4.00E-28 2 G1947 BE342382 BE342382 EST395226 potato stolon, Cornell Un... 2 217 5.20E-28 2 G1947 BG593665 BG593665 EST492343 cSTS Solanum tuberosum cD... 2 223 5.90E-28 2 G1947 BG599468 BG599468 EST504363 cSTS Solanum tuberosum cD... 1 214 6.70E-28 2 G1947 BG890527 BG890527 EST516378 cSTD Solanum tuberosum cD... 3 179 7.00E-28 2 G1947 BG647545 BG647545 EST509164 HOGA Medicago truncatula ... 3 221 1.00E-27 2 G1947 AL380690 AL380690 MtBB53H06F1 MtBB Medicago truncatul... 3 223 1.30E-27 2 G1947 BE488911 BE488911 WHE1077_G04_N07ZS Wheat unstressed ... 1 190 1.40E-27 2 G1947 BE347442 BE347442 sp38d02.y1 Gm-c1043 Glycine max cDN... 1 218 1.50E-27 2 G1947 AW874988 AW874988 00109 leafy spurge Lambda HybriZAP ... 3 194 2.60E-27 2 G1947 BG888216 BG888216 EST514067 cSTD Solanum tuberosum cD... 2 176 2.90E-27 2 G1947 AF235958 AF235958 Medicago sativa heat shock transcri... 3 203 4.00E-27 2 G1947 BG890868 BG890868 EST516719 cSTD Solanum tuberosum cD... 2 215 4.20E-27 2 G1947 AW428882 AW428882 Ljirnpest25-001-b9 Ljirnp Lambda Hy... 3 233 4.50E-27 2 G1947 BM094717 BM094717 saj19h06.y1 Gm-c1066 Glycine max cD... 3 213 4.50E-27 2 G1947 AV415115 AV415115 AV415115 Lotus japonicus young plan... 3 233 5.50E-27 2 G1947 AW738023 AW738023 EST339450 tomato flower buds, anthe... 2 218 5.50E-27 2 G1947 BG646618 BG646618 EST508237 HOGA Medicago truncatula ... 3 232 5.80E-27 2 G1947 BI135911 BI135911 F060P27Y Populus flower cDNA librar... 2 229 7.30E-27 2
LU oo
Figure imgf000119_0001
00 CΛ CΛ -- o co m co o co co oo co oo o co cD co rf o oo ^ co cM ι- rt co r-- co m co co co cn m co m co co co
CM I- - - C0 '>- CM - CM O C0 O 00 O O C0 r-. cn r-. -ι- -^ O CM O 00 CΛ O CM CM ι- σ) O CΛ CM CM CM
CN
Figure imgf000119_0002
•^ S cΛ c -^ co o cM cn cΛ ^ S c ^ ^ n -i- m o o co Λ S o O o S o cn co K Ln o o
» > ^ ie i25 n S w ^ 5 S 58t w 2 N co c c: ? N o !2 S !S o S o> ? ffl S S ™ n ιιl 5 θ 52 S l 2 θ O $ 5 L. l θ S = 0 = o θ = ffl 5 | 52 Lu 2 θ θ S θ r S
CO < CO CQ CQ CQ CO < CQ C0 < C0 < CO < CO CO CO CQ CQ < CQ C0 < < < < CQ CO CQ CQ CQ CQ C0 CQ CQ S S S S S S S ^ S ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ rr ^ t rt t rt rf rt rt f rt f rt
CΛ cn cn cΛ CΛ cn cΛ cn cΛ cΛ CΛ cn cn cΛ CΛ cn cn cΛ cn cn cn cΛ cn cΛ cn cΛ CΛ cn cn cΛ CΛ cn cΛ CΛ CΛ cn δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ Table 2
G1947 BF052865 BF052865 EST438095 potato leaves and petiole... 1 221 1.10E-25 2 G1947 Bl177055 BI177055 EST518000 cSTE Solanum tuberosum cD... 1 226 1.10E-25 2 G1947 BF188654 BF188654 EST444941 potato stolon, Cornell Un... 2 226 1.20E-25 2 G1947 BE643365 BE643365 Cri2_8_J24_SP6 Ceratopteris Spore L... 3 211 1.20E-25 2 G1947 BF650410 BF650410 NF097A06EC1F1039 Elicited cell cult... 1 204 1.20E-25 2 G1947 BG594522 BG594522 EST493200 cSTS Solanum tuberosum cD... 3 226 1.30E-25 2 G1947 BE473183 BE473183 EST418140 potato stolon, Cornell Un... 3 226 1.30E-25 2 G1947 BG450392 BG450392 NF014G01 DT1 F1008 Drought Medicago t... 3 195 1.50E-25 2 G1947 Bl176643 BI176643 EST517588 cSTE Solanum tuberosum cD... 3 181 1.80E-25 2 G1947 AV408054 AV408054 AV408054 Lotus japonicus young plan... 1 209 2.20E-25 2 G1947 BF638833 BF638833 NF079C06PL1 F1040 Phosphate starved ... 2 219 2.40E-25 2 G1947 BI471764 BI471764 sae83d02.y3 Gm-c1065 Glycine max cD... 2 208 2.60E-25 2 G1947 BF648322 BF648322 NF044E12EC1 F1098 Elicited cell cult... 2 203 3.00E-25 2 G1947 BG466138 BG466138 00901 leafy spurge Lambda HybriZAP ... 1 192 3.70E-25 2 G1947 AW703969 AW703969 sk14g08.y1 Gm-c1023 Glycine max cDN... 3 219 3.80E-25 2 G1947 BF644683 BF644683 NF018F07EC1 F1062 Elicited cell cult... 2 203 3.80E-25 2 G1947 AW689532 AW689532 NF021G10ST1 F1000 Developing stem Me... 2 204 4.50E-25 2 G1947 AW596493 AW596493 sj13a09.y1 Gm-c1032 Glycine max cDN... 1 186 5.40E-25 2 G1947 AP003076 AP003076 Oryza sativa genomic DNA, chromosom... 3 203 6.60E-25 2 G1947 GMHSF5 Z46956 G.max mRNA for heat shock transcripti... 1 222 7.00E-25 2 G1947 Bl176663 BI176663 EST517608 cSTE Solanum tuberosum cD... 3 181 7.50E-25 2 G1947 AC111015 AC111015 Oryza sativa chromosome 5 clone OJ1... 3 211 9.50E-25 2 G1947 AU108176 AU108176 AU108176 Rice panicle at flowering ... 3 205 9.60E-25 2 G1947 BE361952 BE361952 DG1_83_G08.b1_A002 Dark Grown 1 (DG... 3 219 1.20E-24 2 G1947 BG039304 BG039304 NXSI_097_F02_F NXSI (Nsf Xylem Side... 3 213 1.20E-24 2 G1947 BI935693 BI935693 EST555582 tomato flower, anthesis L... 3 209 1.40E-24 2 G1947 BE054274 BE054274 GA_Ea0002O16f Gossypium arboreum 7... 1 219 1.90E-24 2 G1947 AV941967 AV941967 AV941967 K. Sato unpublished cDNA I... 2 191 2.50E-24 2 G1947 AB050097 AB050097 Oryza sativa spl7 gene for spl7 pro... 3 198 3.30E-24 2 G1947 AB050098 AB050098 Oryza sativa spl7 mRNA for spl7 pro... 1 194 3.40E-24 2 G1947 BF628773 BF628773 HVSMEb0008B1 Of Hordeum vulgare seed... 3 183 3.50E-24 2 G1947 BM732569 BM732569 sal78h07.y1 Gm-c1061 Glycine max cD... 1 208 3.90E-24 2 G1947 BG452054 BG452054 NF083B04LF1 F1041 Developing leaf Me... 2 204 4.60E-24 2 G1947 BF005633 BF005633 EST434131 DSLC Medicago truncatula ... 1 206 4.60E-24 2 G1947 BE216310 BE216310 HV_CEb0010C13f Hordeum vulgare seed... 3 176 6.60E-24 2 G1947 ZMHSFB X82943 Z.mays mRNA for heat shock factor. 4/... 3 183 6.60E-24 2
CM CM CM CM CM CM CM CM C CM CM CM CM C CM CM CM CM C CM CM CM C CM C CM C C CM UJ LU IJJ LU LU LU LU LIJ U LU LU LU LIJ LU IJJ LU LIJ LU IJJ LU o o o o o o o o o o o o o o o o o o o o o o o o o o o o rf co CD cn cn m cn rf 00 CΛ CM cn CΛ p m p O CO rf CO O) i-; CO m O in in co c cό N O r i- r T^ C CM CM CM C C CO CO CO r^ T-:
OO O r-. i- CΛ O CΛ CO CO CO O CO OO rf OO m OO CO CO CO CD m T- CM '- CO CM CM CM OO m CM -r- CO CO CΛ
O C\| τ- 0) β N O) r - 0) τ- τ- O ι- m (D (O O C C N N S O! 0) N CM CO CO O C\I N CM N r- C\l τ- ι- CM C\l ι- N CM N W '- ι- r- ^ ι- ^ OI C\l '- (\| ι- W W CM - τ- - W ι- τ- w
C0 CΛI C0 ^ ^ CM C0 CO CM ^ CN CO τ- - -ι- C t- CM -ι- 00 CM C Cλl C cp τ_ τ_ cM CO CM CO τ- CM -ι- '- 'i-
Figure imgf000121_0001
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Figure imgf000121_0002
cn cΛ cn cΛ CΛ CΛ CΛ CΛ cn cn cΛ δδδ δδδδδδδδδδδδδδδδ δδ
Figure imgf000121_0003
δδδδδδδδδδ
O) O) O) O) O) O) O) O) O) 00 CO CO CO CO C0 CO CO CO N N S S N N tD lO
LU LU IU m LU LU LU UJ UJ m LU LU UJ LU LU m m LU UJ LU LU LU U LU UJ LU LU LU LU UJ
P O O O O O O O O O O O O O O O O P O O P P O O O O O O O O O P r-. T- T- CM m co oo rt cΛ i^ c c i^ m rf ^ τ- ^ rt r-; -r-; i~ co ρ o co eό c -' ^ r^ T^ W N ^ N ό ^ N N ri ^ β cd N r ^ r-' N W ui lii O i -' -' rf rf co cn r- o co m co m rt rt rt - cθ ι- CM m m co rf co oo oo cM CΛ i- co rt m r-~ m cΛ CΛ θ co cD θ co m m ι-- '^ m m ι- m m m cD m m rf rt rt r^ co co cD co co co rf co co rf m cM CM co co
W N W N '- T- W W W W W N W PI T- I '- N N N - I- N '- '- N N '- W W '- - T- W W W
Figure imgf000122_0001
oo o co og ε
Figure imgf000122_0002
< !i !i f!; t t !i !i 'i ^ ^ ^ ^ ^ ^ |^ ι-- ι-- ^ ι-- ι-- ι^ ^ ι-- ι- r-- r-- r-. f-- r-- t^
S S S S S S S S ^ '^ ^ '^ ^ '^ ^ '^ ^ '^ '^ ^ ^ ^ ^ rf f f rf rt rt rf rt rt rt f t f δδδδδδδδδδδδδδδδδδδδδδδδδδδδoδoδ Table 2
11 G1947 BF264951 BF264951 HV_CEa0010N10f Hordeum vulgare seed... 2 227 1.10E-16 1
11 G1947 BG839442 BG839442 Gm01_17a10_A Gm01_AAFC_ECORC_Glycin. 2 226 1.40E-16 1
11 G1947 AP003682 AP003682 Oryza sativa chromosome 6 clone P04... -1 194 1.50E-16 2
11 G1947 BF187777 BF187777 EST444064 potato stolon, Cornell Un... 3 226 2.60E-16 1
11 G1947 BF597135 BF597135 su95d08.y1 Gm-c1056 Glycine max cDN... 1 226 2.70E-16 1
11 G1947 BF052297 BF052297 EST437527 potato leaves and petiole... 3 226 2.80E-16 1
11 G1947 BG526018 BG526018 56-19 Stevia field grown leaf cDNA ... 3 128 3.80E-16 2
11 G1947 BF067962 BF067962 st79c06.y1 Gm-c1054 Glycine max cDN... 2 223 6.30E-16 1
11 G1947 AW034402 AW034402 EST277973 tomato callus, TAMU Lycop... 2 224 7.00E-16 1
11 G1947 AQ579623 AQ579623 T135087b shotgun sub-library of BAC... -1 221 7.80E-16 1
11 G1947 BG595030 BG595030 EST493708 cSTS Solanum tuberosum cD... 3 219 1.00E-15 1
11 G1947 AW906840 AW906840 EST342963 potato stolon, Cornell Un... 2 220 1.10E-15 1
11 G1947 BI422910 BI422910 EST533576 tomato callus, TAMU Lycop... 1 218 1.10E-15 1
11 G1947 BE433610 BE433610 EST400139 tomato breaker fruit, TIG... 3 218 1.30E-15 1
11 G1947 BE019974 BE019974 sm38b12.y1 Gm-c1028 Glycine max cDN... 2 218 1.40E-15 1
11 G1947 AI895834 AI895834 EST265277 tomato callus, TAMU Lycop... 2 218 1.60E-15 1
11 G1947 AI725043 AI725043 942 PtlFG2 Pinus taeda cDNA clone 8... 3 216 2.10E-15 1
11 G1947 BJ178487 BJ178487 BJ178487 normalized full length cDN... 2 216 2.20E-15 1
Is) Is) 11 G1947 BH491276 BH491276 BOGTM88TR BOGT Brassica oleracea ge... 1 214 3.30E-15 1
11 G1947 BI993532 BI99353201473 leafy spurge Lambda HybriZAP ... 3 214 3.70E-15 1
11 G1947 BE354387 BE354387 EST355730 tomato flower buds, anthe... 2 214 4.80E-15 1
11 G1947 BJ178399 BJ178399 BJ178399 normalized full length cDN... 3 212 6.00E-15 1
11 G1947 BJ174030 BJ174030 BJ174030 normalized full length cDN... 2 212 6.30E-15 1
11 G1947 BJ183254 BJ183254 BJ183254 normalized full length cDN... 2 212 6.60E-15 1
11 G1947 BM098700 BM098700 EBem08_SQ003_N07_R IGF Barley EBemO... 2 150 8.10E-15 2
11 G1947 BE920963 BE920963 EST424732 potato leaves and petiole... 3 209 1.30E-14 1
11 G1947 BF650501 BF650501 NF097H11 EC1 F1094 Elicited cell cult... 2 209 1.30E-14 1
11 G1947 BF097217 BF097217 EST400595 tomato nutrient deficient... 3 209 1.40E-14 1
11 G1947 AI896906 AI896906 EST266349 tomato callus, TAMU Lycop... 3 146 1.50E-14 2
11 G1947 AB014484 AB014484 Nicotiana tabacum NtHSF2 mRNA for h... 3 220 2.40E-14 1
11 G1947 BE020791 BE020791 sm52h09.y1 Gm-c1028 Glycine max cDN... 2 206 3.30E-14 1
11 G1947 AI055366 AI055366 coau0003M17 Cotton Boll Abscission ... 1 204 3.90E-14 1
11 G1947 AW696868 AW696868 NF111 F05ST1 F1046 Developing stem Me... 1 204 4.00E-14 1
11 G1947 AW686271 AW686271 NF039H06NR1F1000 Nodulated root Med... 3 204 4.20E-14 1
11 G1947 BF643550 BF643550 NF026D02EC1F1015 Elicited cell cult... 3 203 4.80E-14 1
11 G1947 BF648049 BF648049 NF025G04EC1 F1035 Elicited cell cult... 2 203 5.70E-14 1
IJJ LU W LU LU LU LU LU LU W LU LU LU LU LU LU IJJ LU IJJ IJJ LU IJJ LU IJJ LU W ooι no ^o ooι oιooιooιθoτ- No o on o ηo o* o* oιoισS fflo ^o 'o- No oco fflo *o o ^o oq oη ιoo oo fflo ^o oιβoN Np p iri ω ^ w ri ri ri ^' iri iό cD cό ed ffl ^ T^ T-' ^ w ri c^ ^ i ^ T^ w w w ri t co co -ι- f-. rs. r-. o cD CD m rt rf co o o - cn o cD f-- co rt rf co cM rf θ - o cM CD m m P o o) cn cn o o) 0) 0) 0) cn cn cn T-- o) 0) 0) oo o) co oo oo o) o oo oo o) O oo oo co r-- -»— - T-
N « « ι- ι- ι- N r ι- ι- ι- r - r N τ- f i- r ι- r f - r m- ι- N r r ι- ι- r r r
W CM W ι- CO CM CO W CO C '- τ- 7 - CO CM C0 '- ι- CO ι- C0 c? OJ C0 '- τ- τ- C N '- r- τ- CM N W
Figure imgf000124_0001
£ co <SmLm3<δco?§co5<Sm coLm?§coLc?Q2<5<δcQ£LOsmimico <ico£m mL?mLξcoicQ <L?i<LtιiθLc§Q§CD§CQ co
S S ^ S S S ^ S ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ r rt rt rf rt t rt f rf rf rt t t rt rf rf cn o) 0) 0) 0) 0) o) 0) 0) 0) 0) 0) 0) 0) cn o) 0) 0) 0) 0) 0) 0) 0) o) 0) cn o) 0) cn o) 0) 0) p o) 0) a δδδδδδδδδδδδδδδδδδδδ δδδδδδδδδδδδδδδ CM
0 0 0 0 0 0 0 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 00 00 00 00 00 00 00 00 00 00 r-. r*. [ . r- r-. r-- r- ι- τ- ι- -^ -^- -^ - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 o o o o o P o
UJ UJ LU UJ LU LU UJ LU LU UJ LU LU UJ LU LU UJ LU LU LU UJ LU LU LU LU LU LU IJJ LU UJ LU LU IJJ LU UJ LU
P P O O O P O O O O O O O O o o o o o O O O O O O O- O- - o ~ o~ o o _ o_ o —
CM co co cn co cD co co m m oo cΛ oo m co cn cD CM m co co co co cD cn P CM O O) - r- 00 CO ι- t- ι- - rt m CD CM CM CM CM CO rt m co ι-. oo ι- τ- ^ ι- cM m m oo oo -1- ι- rt m m m cD m rf m rf i- C0 CM r-- C0 rf CD C0 C0 C0 CM CM C0 0) O 00 00 C0 C0 rf 00 -^ CM O i- ->- 00 r . rt C0 CM r- ι- r-- rs. (s. fs. r-- r-- co co cM τ- cD co co cD co cD m co m co m m m m m m cD m rt o rt rt rf m
w M τ- W cM CO CM CM CO ^ ^ CO CM CM C cM - CM CM -ι- τ- CM CM ->- C0 -ι- -- -ι- CO CO C0 -ι- C0 CM 'i- C0
Figure imgf000125_0001
f~ r^ | r-. r^ fs r . r . rs r-. rs. rs. r. . r rs. r |s. rs |s. rs. rs. r. r~ rs rs. f^
0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0 0) 0) 0) δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ
o o o o o o O O O O O O O P O O O O O O P O P O ι- -^- -ι- ι- CM CM CM CM CM CO rf
LU UJ LU LU LU LU LU UJ LU LU LU UJ UJ LU LU LU LU LU LU LU LU LU LU LU LU O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O c τ- cM co oo c co o rs. co τ- oo τ- c rt rt rs. o co cn oo oo oo oo cM q q q q q q q q
m o o o c |s. m m o m ω cΛi σ co o ι-- rt cΛ oo r. fs. rs. f . CM cn cM CM m c cn rt cM oo o m rf rt rf CO CO CO CO rt rf CO CO CO rt CO CO CO CM CM CM CO CO CO CO CO CO CM CO CM CO 'i- CO CO CM CM CO
W C0 C CO C0 ι- τ- Cθ W w ^ « ι- 'r C0 - 0) ι- N C0 τ- OJ Cι| >- C0 C C C0 W C0 W c C N -
Figure imgf000126_0001
oo CM -. rt o
CO CO CM CD CM 00 Cn o ∞ CO O CO iL l W 2 cl3 ϋ 5 S l l L 5 § w § S o O C3 g g § g g <o S g 2 ^ 22 23 § 2 co cQ m cQ CQ m m cQ m cQ m < m < < < cQ CQ < cQ < < < < < cD CQ CQ CQ cQ co < < co fc fc fc fc fc ^ r-■ ι^ ^-" ^ ^ '-- '-- r^ ι^ ιs- '-- ι-- ι-- ι ι-- f-■ - ^s■ ^s. |s. r-- rs. ^s. ^. ^s, fs. |. ιs. ιs^
2 3 5 S S S ^ ^ '* ^ ^ ^ "* '* '* '* '* '* ^ '* ^ ^ t rt rt rt rf rt rt rf rt rf rt rt f rt 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ δ Table 2
11 G1947 BI951809 BI951809 HVS Em0003A03f Hordeum vulgare gree... 2 134 0.00043 1
11 G1947 BF188095 BF188095 EST444382 potato stolon, Cornell Un... 2 118 0.0005 1
11 G1947 BI922715 BI922715 EST542619 tomato callus Lycopersico... 2 130 0.00053 1
11 G1947 AW034135 AW034135 EST277706 tomato callus, TAMU Lycop... 3 129 0.00056 1
11 G1947 AW307441 AW307441 sf57a06.y1 Gm-c1009 Glycine max cDN... 2 128 0.00057 1
11 G1947 AW775388 AW775388 EST334453 DSIL Medicago truncatula ... 2 116 0.00061 1
11 G1947 BM403958 BM403958 EST578285 P. infestans-challenged p... 2 130 0.00067 1
11 G1947 BE459508 BE459508 EST414800 tomato developing/immatur... 1 128 0.00068 1
11 G1947 A 738402 AW738402 EST339829 tomato flower buds, anthe... 2 126 0.00091 1
11 G1947 Bl136008 BI136008 F061 P77Y Populus flower cDNA librar... 3 118 0.0011 1
11 G1947 BI305271 BI305271 NRS2R_1_I04 Drought stress (root) O... 1 113 0.0012 1
11 G1947 AZ048458 AZ048458 PSB119 Barley Pstl genomic clones H... -1 129 0.0016 1
11 G1947 BH459792 BH459792 BOHIE27TF BOHI Brassica oleracea ge... -1 126 0.0021 1
11 G1947 AP003619 AP003619 Oryza sativa chromosome 6 clone P05... 1 130 0.0023 1
11 G1947 AP003763 AP003763 Oryza sativa chromosome 6 clone P05... 1 130 0.0023 1
11 G1947 BE433847 BE433847 EST404925 tomato breaker fruit, TIG... 1 123 0.0025 1
11 G1947 AW030642 AW030642 EST273897 tomato callus, TAMU Lycop... 3 109 0.0034 1
11 G1947 BJ181329 BJ181329 BJ181329 normalized full length cDN... 1 120 0.0035 1
Is) βs 11 G1947 BH227732 BH227732 1006141 G09.x1 1006 - RescueMu Grid ... 2 110 0.0043 1
11 G1947 BM098554 BM098554 EBem08_SQ003_G12_R IGF Barley EBemO... 3 109 0.0046 1
11 G1947 B 324930 BM324930 PIC1_37_F08.b1_A002 Pathogen-infect... 1 109 0.0048 1
11 G1947 BI425195 BI425195 saf89d01.y3 Gm-d 079 Glycine max cD... 1 117 0.0071 1
11 G1947 BF293856 BF293856 WHE2153_G07_N13ZS Triticum turgidum... 2 107 0.0081 1
11 G1947 BH631347 BH631347 1007081 C06.x2 1007 - RescueMu Grid ... -1 111 0.0082 1
11 G1947 BF596658 BF596658 su61c12.y1 Gm-c1069 Glycine max cDN... 2 107 0.0084 1
11 G1947 BH712358 BH712358 BOHWN47TF BO_2_3_KB Brassica olerac... 3 121 0.0099 1
11 G1947 AW035844 AW035844 EST281998 tomato callus, TAMU Lycop... 1 116 0.013 1
11 G1947 BE323462 BE323462 NF008F08PL1 F1063 Phosphate starved ... 3 104 0.014 1
11 G1947 BE941244 BE941244 EST420823 MGHG Medicago truncatula ... 3 113 0.039 1
11 G1947 BM731247 BM731247 sal69e10.y1 Gm-c1061 Glycine max cD... 3 112 0.042 1
11 G1947 BI921385 BI921385 EST541288 tomato callus Lycopersico... 1 112 0.045 1
11 G1947 BG511416 BG511416 sad17e08.y1 Gm-c1074 Glycine max cD... 2 108 0.059 1
11 G1947 AW035854 AW035854 EST282008 tomato callus, TAMU Lycop... 1 111 0.061 1
11 G1947 BG096296 BG096296 EST460815 potato leaves and petiole... 1 98 0.072 1
11 G1947 BE643441 BE643441 Cri2_8_N06_SP6 Ceratopteris Spore L... 2 113 0.074 1
11 G1947 BF648760 BF648760 NF049E04EC1F1034 Elicited cell cult... 3 97 0.077 1
Table 2
1 G1947 BG155529 BG155529 sab45c09.y1 Gm-c1026 Glycine max cD... 3 97 0.08
1 G1947 AW399021 AW399021 EST309521 L. pennellii trichome, Co... 3 110 0.095
1 G1947 A 835041 AV835041 AV835041 K. Sato unpublished cDNA I... 1 106 0.16
1 G1947 BH578768 BH578768 BOGUE71TF BOGU Brassica oleracea ge... 2 104 0.31
1 G1947 AW686674 AW686674 NF040F05NR1 F1000 Nodulated root Med... 2 100 0.42
1 G1947 AW035621 AW035621 EST281359 tomato callus, TAMU Lycop... 2 103 0.44
1 G1947 BM111527 BM111527 EST559063 potato roots Solanum tube... 3 105 0.45
1 G1947 BF506983 BF506983 12162P-5 Pooled green leaf and root... -3 94 0.54
1 G1947 BI643645 BI643645 DG1_92_E12.b1_A002 Dark Grown 1 (DG... 1 89 0.83
1 G1947 AW620962 AW620962 sj98b03.y1 Gm-c1023 Glycine max cDN... 2 84 0.9
1 G1947 BH537567 BH537567 BOGJK94TR BOGJ Brassica oleracea ge... -3 99 0.91
1 G1947 C73168 C73168 C73168 Rice panicle at flowering stag... 2 87 0.98
1 G1947 BH537559 BH537559 BOGJK94TF BOGJ Brassica oleracea ge... 1 96 0.996
1 G1947 AP003573 AP003573 Oryza sativa chromosome 6 clone P05... 3 99 0.997
1 G1947 AP003571 AP003571 Oryza sativa chromosome 6 clone P04... -1 99 0.997
1 G1947 BM375417 BM375417 EBem06_SQ003_E14_R IGF Barley EBemO... 2 91 0.999
1 G1947 AZ126806 AZ126806 OSJNBb0079M09r CUGI Rice BAC Librar... -1 88 0.9993 h-i 1 1 G1947 BH674996 BH674996 BOMLL87TF BO_2_3_KB Brassica olerac... -3 92 0.9997
~4 1 1 G1947 GI-19490 heat stress transcription factor HSF30 -2 246 5.90E-45 2
1 G1947 GI-100265 525480 heat shock transcription factor HSF... -2 246 5.90E-45 2
1 G1947 GI-729774 HSF3_LYCPE HEAT SHOCK FACTOR PROTEIN HSF30... -2 246 5.90E-45 2
1 G1947 GI-8347238 AF208544_1 heat stress transcription facto... -2 253 2.00E-40 2
1 G1947 GI-662924 heat shock transcription factor 21 -1 234 2.20E-37 2
1 G1947 GI-2129828 S59537 heat shock transcription factor 21 ... -1 234 2.20E-37 2
1 G1947 GI-2130133 S61458 heat shock factor (clone hsfa) - ma... -1 238 1.60E-33 2
1 G1947 GI-19492 heat shock transcription factor 8 -1 237 3.30E-33 2
1 G1947 GI-100264 525481 heat shock transcription factor 8 -... -1 237 3.30E-33 2
1 G1947 GI-729775 HSF8J.YCPE HEAT SHOCK FACTOR PROTEIN HSF8 ... -1 237 3.30E-33 2
1 G1947 GI-19260 heat stress transcription factor 8 -1 237 4.20E-33 2
1 G1947 GI-100225 S25478 heat shock transcription factor 8 -... -1 237 4.20E-33 2
1 G1947 GI-11386827 HSF8J.YCES HEAT SHOCK FACTOR PROTEIN HSF8 ... -1 237 4.20E-33 2
1 G1947 GI-16118447 heat shock transcription factor [Phaseolus... -1 220 2.40E-32 2
1 G1947 GI-5821138 heat shock factor -1 220 1.00E-30 2
1 G1947 GI-7158882 AF235958_1 heat shock transcription factor -1 203 8.70E-30 2
1 G1947 GI-3550552 heat shock transcription factor (HSFA) -1 247 2.00E-29 2
1 G1947 GI-7488808 T06534 heat shock transcription factor A -... -1 247 2.00E-29 2
Table 2
11 G1947 GI-14209551 putative heat shock factor [Oryza sativa] -2 200 2.40E-29 3
11 G1947 GI-662930 heat shock transcription factor 34 212 8.50E-29 2
11 G1947 GI-2129831 559538 heat shock transcription factor 34 ... 212 8.50E-29 2
11 G1947 GI-16580739 heat stress transcription factor Spl7 [Ory... 207 2.20E-28 2
11 G1947 GI-16580741 heat stress transcription factor Spl7 [Ory... 207 2.20E-28 2
11 G1947 GI-11761077 putative heat shock factor protein 1 (HSF ... 200 4.50E-28 3
11 G1947 GI-19488 heat stress transcription factor 204 5.70E-28 3
11 G1947 GI-100267 S12361 heat shock transcription factor HSF... 204 5.70E-28 3
11 G1947 GI-123684 HSF2_LYCPE HEAT SHOCK FACTOR PROTEIN HSF24... 204 5.70E-28 3
11 G1947 GI-671868 heat shock transcription factor 5 222 4.00E-27 2
11 G1947 GI-2129832 559539 heat shock transcription factor 5 -... 222 4.00E-27 2
11 G1947 GI-16580743 spl7 protein [Oryza sativa] 194 1.70E-26 2
11 G1947 GI-16580745 spl7 protein [Oryza sativa] 194 1.70E-26 2
11 G1947 GI-886742 heat shock factor 179 2.10E-26 2
11 G1947 GI-1362193 S57633 heat shock factor - maize 179 2.10E-26 2
11 G1947 GI-2130134 S61448 heat shock factor (clone hsfb) - maize 179 2.10E-26 2
11 G1947 GI-2130135 S61459 heat shock factor (clone hsfc) - ma... 185 1.30E-25 2
11 G1947 GI-5821136 heat shock factor 207 6.60E-25 2
Is)
00 11 G1947 GI-3550588 heat shock transcription factor (HSFA) 256 1.OOE-24 2
11 G1947 GI-7488809 T06535 heat shock transcription factor A -... -2 256 1.OOE-24 2
11 G1947 GI-15624016 putative heat shock transcription factor [... 170 3.50E-24 2
11 G1947 GI-662928 heat shock transcription factor 33 132 2.10E-17 2
11 G1947 GI-671866 heat shock transcription factor 29 -2 107 1.30E-12 3
11 G1947 GI-2129829 S59541 heat shock transcription factor 29 ... -2 107 1.30E-12 3
11 G1947 GI-671867 heat shock transcription factor 31 -2 124 1.40E-11 2
11 G1947 GI-2129830 559540 heat shock transcription factor 31 ... -2 124 1.40E-11 2
11 G1947 GI-169961 G-box binding factor -2 100 0.031
11 G1947 GI-7488677 T07151 G-box binding factor 2B - soybean (... -2 100 0.031
11 G1947 GI-169959 G-box binding factor -2 100 0.039
11 G1947 GI-7488676 T07150 G-box binding factor 2A - soybean (... -2 100 0.039
11 G1947 GI-1155054 regulator of MAT2 -2 98 0.064
11 G1947 GI-7488785 T10985 regulator protein ROM2 - kidney bean -2 98 0.064
11 G1947 GI-1399007 transcription factor -2 88 0.51
11 G1947 GI-7488451 T07887 G box-binding protein homolog GBF1 ... -2 88 0.51
11 G1947 GI-1399005 transcription factor -2 88 0.51
11 G1947 GI-7488452 T07882 G box-binding protein homolog GBF2 ... -2 88 0.51
Table 2
11 G1947 GI-1771158 MFP1 protein -2 89 0.72
11 G1947 GI-7489035 T07111 MAR binding filament-like protein 1... -2 89 0.72
11 G1947 GI-14423760 MFP1J.YCES MAR BINDING FILAMENT-LIKE PROTE... -2 89 0.72
11 G1947 GI-2280516 BYJ14 -2 74 0.91
11 G1947 GI-7489186 T02225 protein BYJ14 - common tobacco (fra... -2 74 0.91
11 G1947 GI-7108715 AF131231_1 MAR-binding protein MFP1 homolog -2 83 0.998
11 G1947 GI-14423763 MFP1_TOBAC MAR BINDING FILAMENT-LIKE PROTE... -2 83 0.998
11 G1947 GI-2224398 ORF57c 2 57 0.999
11 G1947 GI-7515391 T07235 hypothetical protein 57c - Chlorell... 2 57 0.999
11 G1947 GI-7524805 ORF57c [Chlorella vulgaris] 2 57 0.999
11 G1947 GI-728626 TAF-2 -2 79 0.9997
11 G1947 GI-1076623 S53020 G-box-binding protein TAF-2 - commo... -2 79 0.9997
13 G152 AP004766 AP004766 Oryza sativa (japonica cultivar-gro... 254 2.10E-28 4
13 G152 OSJN00003 AL606460 Oryza sativa chromosome 4 clone OSJ... 246 1.00E-27 4
13 G152 AX085151 AX085151 Sequence 1 from Patent WO0112798. 3... 234 1.30E-27 3
13 G152 AX085348 AX085348 Sequence 1 from Patent WO0112799. 3... 234 1.30E-27 3
13 G152 AF112149 AF112149 Zea mays MADS box protein 2 (mads2)... 234 1.30E-27 3
13 G152 AMDEFH125 Y10750 A.majus mRNA for DEFH125 protein. 8/1997 244 2.80E-26 2
Is) sO 13 G152 BM405213 BM405213 EST579540 potato roots Solanum tube... 2 239 7.60E-26 2
13 G152 AI974336 AI974336 T110185e KVO Medicago truncatula cD... 2 239 1.80E-25 2
13 G152 AW218280 AW218280 EST303461 tomato radicle, 5 d post-... 3 232 2.90E-25 2
13 G152 AW706936 AW706936 sk08d08.y1 Gm-c1023 Glycine max cDN... 2 235 1.OOE-24 2
13 G152 AP004526 AP004526 Lotus japonicus genomic DNA, chromo... -2 243 8.40E-24 3
13 G152 MSU91964 U91964 Medicago sativa MADS-box protein mRNA... 1 212 1.50E-23 2
13 G152 AP003868 AP003868 Oryza sativa chromosome 8 clone OJ1... 3 247 2.70E-22 3
13 G152 BE354988 BE354988 DG1_10_H09.b1_A002 Dark Grown 1 (DG... 2 230 3.40E-22 2
13 G152 AL389596 AL389596 MtBC56A01 F1 MtBC Medicago truncatul... 2 206 9.20E-22 2
13 G152 AX085162 AX085162 Sequence 12 from Patent WO0112798. ... 1 240 1.00E-20 2
13 G152 AX085359 AX085359 Sequence 12 from Patent WO0112799. ... 1 240 1.00E-20 2
13 G152 BH743480 BH743480 gz74f05.g1 BoBudsOI Brassica olerac... -1 190 1.30E-18 2
13 G152 BM268642 BM268642 MEST398-C12.univ ISUM5-RN Zea mays ... -3 252 7.40E-18 1
13 G152 BM334225 BM334225 MEST134-C05.T3 ISUM5-RN Zea mays cD... -1 252 8.50E-18 1
13 G152 AF396746 AF396746 Ipomoea batatas MADS-box protein (M... 1 198 4.10E-17 2
13 G152 AQ917367 AQ917367 T233384b Medicago truncatula BAC Ii... 2 245 5.00E-17 1
13 G152 BE319808 BE319808 NF020A03RT1F1017 Developing root Me... 3 240 1.90E-16 1
13 G152 BE596704 BE596704 PI1_58_F04.b1_A002 Pathogen induced... 1 238 2.50E-16 1
LU LU LU LU LU LU LU LU LU LU LU LU LU UJ IJJ LU LU LLI UJ o o o o o o o o o o o o o o o o o o o o o o o o o o o σ o o o o o o p p o oo cλi co co co π i^ oo m ri- co ^ c m q cΛi co rr rr m cq cq r-: ^
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m
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CD CD CQ CQ CD CQ < CQ ≤ CO < < < CO < < m < < CQ CQ CQ C0 lJ CD < < < C0 ζJ CM CM CM CM CM CM CM CM CM CM CM CM CM C C CM CM CM CM CM CM CM CM CM CM CM C CM C CM CM W m m m m m m m m m m m m m m m m m m m m m m m in LO Lo m m Lo in o m m Ln LO δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ Table 2
G152 BH744278 BH744278 gt35a01.b1 BoBudsOI Brassica olerac... 1 209 3.20E-13
G152 AF101420 AF101420 Cichorium intybus MADS box protein ... 1 210 3.30E-13
G152 AI437956 AI437956 sa41g11.y1 Gm-c1004 Glycine max cDN... 3 209 3.40E-13
G152 AF150933 AF150933 Physcomitrella patens MADS-domain p... 2 227 3.50E-13
G152 BE315880 BE315880 NF027E08LF1F1055 Developing leaf Me... 1 210 4.10E-13
G152 AW186417 AW186417 se67e07.y1 Gm-c1019 Glycine max cDN... 3 207 4.80E-13
G152 AW309115 AW3091 15 sf93e09.y1 Gm-c1019 Glycine max cDN... 3 207 5.10E-13
G152 AF150934 AF150934 Physcomitrella patens MADS-domain p... 1 227 5.60E-13
G152 AI894541 AI894541 EST263984 tomato callus, TAMU Lycop... 1 209 5.80E-13
G152 AW010398 AW010398 ST06D02 Pine TriplEx shoot tip libr... 3 206 5.90E-13
G152 BE659970 BE659970 314 GmaxSC Glycine max cDNA, mRNA s... 2 207 6.20E-13
G152 AF150932 AF150932 Physcomitrella patens MADS-domain p... 1 226 6.60E-13
G152 BI926036 BI926036 EST545925 tomato flower, buds 0-3 m... 1 207 7.00E-13
G152 AW400298 A 400298 707057E09.X1 707 - Mixed adult tiss... 1 205 7.40E-13
G152 BF484557 BF484557 WHE2317_A03_A05ZS Wheat pre-anthesi... 1 205 7.90E-13
G152 BE610259 BE610259 sq52a10.y1 Gm-c1019 Glycine max cDN... 2 206 7.90E-13
G152 BE493790 BE493790 WHE1275_E07_I13ZS Secale cereale an... 2 205 8.00E-13
G152 BG445079 BG445079 GA Ea0026K23f Gossypium arboreum 7... 3 204 8.00E-13
G152 AI731368 AI731368 BNLGHJ9370 Six-day Cotton fiber Gos... 2 204 8.20E-13
G152 AI731375 AI731375 BNLGHι'9382 Six-day Cotton fiber Gos... 2 204 8.30E-13
G152 BE918053 BE918053 OV1_1_C11.b1_A002 Ovary 1 (OV1) Sor... 1 205 8.70E-13
G152 BG441266 BG441266 GA Ea0012l13f Gossypium arboreum 7... 2 204 8.70E-13
G152 BE918762 BE918762 OV1_4_F11.b1_A002 Ovary 1 (OV1) Sor... 1 205 8.90E-13
G152 BG357222 BG357222 OV2_11_C11.b1_A002 Ovary 2 (OV2) So... 3 205 9.10E-13
G152 AB022666 AB022666 Gnetum parvifolium GpMADS4 mRNA, co... 1 221 9.30E-13
G152 BE249689 BE249689 NF020D04LF1F1041 Developing leaf Me... 3 208 9.40E-13
G152 BI263356 BI263356 NF089C12PL1 F1098 Phosphate starved ... 1 204 9.40E-13
G152 BG440218 BG440218 GA Ea0006G19f Gossypium arboreum 7... 2 204 9.40E-13
G152 AF107588 AF107588 Dendrobium grex Madame Thong-IN put... 2 204 9.40E-13
G152 BG456966 BG456966 NF098D09PL1F1076 Phosphate starved ... 2 204 9.50E-13
G152 BI098309 BI098309 IP1_30_G10.b1_A002 Immature pannicl... 3 205 9.60E-13
G152 BE495206 BE495206 WHE1269_F04_L07ZS Secale cereale an... 1 205 9.70E-13
G152 AI725968 AI725968 BNLGHM3738 Six-day Cotton fiber Go... 2 204 9.70E-13
G152 BE455888 BE455888 HVSMEg0017B20f Hordeum vulgare pre-... 1 203 9.90E-13
G152 BG525865 BG525865 53-44-1-2 r Stevia field grown leaf... 1 203 1.00E-12
G152 AI729115 AI729115 BNLGHH2679 Six-day Cotton fiber Go... 2 204 1.00E-12
LLI LU L1J IJJ LU LU |JJ LU |JJ W LU W LU LU LU LU LU LU W
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C CM CM CO CO CO C ^ CM C CM CM CO CM CM ι- τ- CM CM - CO CM CM -r- -ι- CO CM '>- CM C CM τ- CO CM CO
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Figure imgf000133_0001
S ^ S |5 | cM rr co 5° « § ^ fe ^ S S co fe « S a fe r? cS 8
m < < m ω < < m < < m ω < ω < < < ω o m < m m cQ cQ < < co co cQ ω co co < CQ
CjJ CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM C CM CM C m m m m m m m m m m m m m m m m m m m m m m m in m in m LO LO Lo m m m m m m δδδδδδδδδδδδδδδδδδδδδδδ δδδδδδδδδδδδ co co co co co co co co co co co co n co co n co co co co co co co co co co co co co co co co co co co co
CΛI CM CN CM CM CM CM CM CM CM CM CM CM CΛI CM CM CM C CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM LoU LoU Lo LoU IoJJ LoU LoIJ LoJJ LoU IoJJ LoU Wo LoIJ LoU Wo IoU LoU LoU LoU LoU o o o o o o o o o o o o o o o o rr m m m m m m m m m m m m co m m rs- |s. rs. rs. rs. |s. rs- |s. oo oo oo oo oo oo o) 0) 0) 0) 0) -i-;
CO CO CM CO CO CO CO CO CO CO CO CO CO CO CM CO CO CM CO CO CO T- CO CO rr CO CO CO CO CM CO CO CO CO CM O O O O CM O O O O O O O CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM
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Figure imgf000134_0001
2 |iU LlU LlUgLUFt Ss|S§UlU S|Ϊ 5§£I? ιIϊ|§|§l5 §l2s2l»§I2ϊf|LU|W m cO CO CO CD < < < CQ CO < < < CQ < < < < < < < < < CQ Q
Figure imgf000134_0002
C CM CM CM CM CM CM CM C CM CM CM CM CM C CM CM CM CM CM CM CM CM Cλl CM CM CM CM W m m m m m m m m m m m m m m m m m m m m m m in Lo Lo in rn m LO LO O LO in δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ
CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO Table 2
G152 BG412085 BG412085 OV2_37_G09.b1_A002 Ovary 2 (OV2) So... 3 202 2.10E-12 1
G152 BM535524 BM535524 EST588557 tomato breaker fruit Lyco... 2 203 2.10E-12 1
G152 BF276744 BF276744 GA Eb0030l01f Gossypium arboreum 7... 2 202 2.10E-12 1
G152 BM436799 BM436799 VVA010B05_53181 An expressed sequen... 3 201 2.10E-12 1
G152 AI794686 AI794686 sb67f02.y1 Gm-c1019 Glycine max cDN... 3 201 2.10E-12 1
G152 BE321182 BE321182 NF021E06IN1F1039 Insect herbivory M... 3 205 2.10E-12 1
G152 BG262504 BG262504 WHE0936_G06_N12ZS Wheat 5-15 DAP sp.. 1 202 2.20E-12 1
G152 LETDR8 X60760 Lesculentum TDR8 mRNA. 3/1992 3 200 2.20E-12 1
G152 BG138098 BG138098 EST478540 wild tomato pollen Lycope... 2 201 2.20E-12 1
G152 BI311095 BI311095 EST5312845 GESD Medicago truncatula... 2 200 2.20E-12 1
G152 BE637049 BE637049 WHE1821-1824_G05_G05ZS Secale cerea... 1 203 2.20E-12 1
G152 BM359874 BM359874 GA Ea0024M3r Gossypium arboreum 7... 2 202 2.20E-12 1
G152 GGN251556 AJ251556 Gnetum gnemon mRNA for putative MAD... 1 216 2.20E-12 1
G152 BF201770 BF201770 WHE1765_E01_J01ZS Wheat pre-anthesi... 3 203 2.20E-12 1
G152 BG445408 BG445408 GA Ea0028G20f Gossypium arboreum 7... 3 209 2.30E-12 1
G152 BG139571 BG139571 EST480109 wild tomato pollen Lycope... 2 201 2.30E-12 1
G152 AF097747 AF097747 Cryptomeria japonica putative MADS-... 1 200 2.40E-12 1
G152 BF622888 BF622888 HVSMEa0009O05f Hordeum vulgare seed... 2 200 2.40E-12 1
G152 BE659918 BE659918 1299 GmaxSC Glycine max cDNA, mRNA ... 2 200 2.50E-12 1
G152 BE585982 BE585982 Est#7pT7_B09_b9_073 KSU wheat Fusar... 1 200 2.60E-12 1
G152 AI161447 AM 61447 A001 P09U Hybrid aspen plasmid libra... 3 203 2.60E-12 1
G152 BI935126 BI935126 EST555015 tomato flower, anthesis L... 3 199 2.70E-12 1
G152 AW277843 AW277843 sf87d01.y1 Gm-c1019 Glycine max cDN... 1 200 2.70E-12 1
G152 BF112397 BF112397 EST439987 tomato breaker fruit Lyco... 3 203 2.70E-12 1
G152 AF042068 AF042068 Medicago sativa MADS-box protein NM... 2 221 2.70E-12 1
G152 A36587 A36587 Sequence 1 from Patent WO9400582. 3/1997 2 199 2.70E-12 1
G152 PETTRNSFA M91190 Petunia transcription factor (fbpl) R... 2 199 2.70E-12 1
G152 NTGLOBOSA X67959 N.tabacum GLO mRNA. 6/1993 1 199 2.80E-12 1
G152 BE193581 BE193581 HVSMEh0081 M10f Hordeum vulgare 5-45... 2 199 2.80E-12 1
G152 BM412354 BM412354 EST586681 tomato breaker fruit Lyco... 3 199 2.90E-12 1
G152 Bl176052 BI176052 EST517135 cSTS Solanum tuberosum cD... 1 199 3.00E-12 1
G152 BG599624 BG599624 EST504519 cSTS Solanum tuberosum cD... 1 199 3.00E-12 1
G152 BI272847 BI272847 NF097G09FL1 F1071 Developing flower ... 3 200 3.00E-12 1
G152 AF198176 AF198176 Dendrobium grex Madame Thong-IN MAD... 3 199 3.10E-12 1
G152 AW928593 AW928593 EST337381 tomato flower buds 8 mm t... 2 203 3.10E-12 1
G152 BF728454 BF728454 1000062F09.x3 1000 - Unigene I from... 3 202 3.20E-12 1
Table 2
G152 BI933057 BI933057 EST552946 tomato flower, 8 mm to pr... 3 199 3.20E-12
G152 AI486208 AI486208 EST244529 tomato ovary, TAMU Lycope... 2 199 3.30E-12
G152 BM405355 BM405355 EST579682 potato roots Solanum tube... 1 199 3.40E-12
G152 AW737591 AW737591 EST339018 tomato flower buds, anthe... 1 199 3.50E-12
G152 AW934007 AW934007 EST359850 tomato fruit mature green... 1 199 3.50E-12
G152 AW934390 AW934390 EST360233 tomato fruit mature green... 1 199 3.50E-12
G152 BG054384 BG054384 OV2_3_G05.b1_A002 Ovary 2 (OV2) Sor... 3 202 3.50E-12
G152 BE431166 BE431166 SUN012.B10F991223 ITEC SUN Wheat cD... 1 199 3.50E-12
G152 AI485336 AI485336 EST243657 tomato ovary, TAMU Lycope... 1 199 3.60E-12
G152 AF335234 AF335234 Petunia x hybrida MADS-box transcri... 1 214 3.60E-12
G152 BE918068 BE918068 OV1_1_E07.b1_A002 Ovary 1 (OV1) Sor... 2 202 3.60E-12
G152 BG446848 BG446848 GA Eb0039l21f Gossypium arboreum 7... 2 199 3.60E-12
G152 AW219022 AW219022 EST301504 tomato root during/after ... 3 199 3.70E-12
G152 AW934066 AW934066 EST359909 tomato fruit mature green... 3 200 3.70E-12
G152 AF198174 AF198174 Dendrobium grex Madame Thong-IN MAD... 2 204 3.70E-12
G152 AF381767 AF381767 Mimulus nasutus apetala3-like prote... 1 200 3.70E-12
G152 LETDR3 X60756 L.esculentum TDR3 mRNA. 3/1992 3 199 3.70E-12
G152 BF657741 BF657741 OV2_17_D12.b1_A002 Ovary 2 (OV2) So... 3 199 3.70E-12
G152 BM136198 BM136198 WHE2606_E05_I10ZS Wheat Fusarium gr... 1 201 3.80E-12
G152 AW932707 AW932707 EST358550 tomato fruit mature green... 3 199 3.80E-12
G152 BE430753 BE430753 SUN007.H09F991221 ITEC SUN Wheat cD... 1 209 3.80E-12
G152 BE460685 BE460685 EST412104 tomato breaker fruit, TIG... 3 199 3.80E-12
G152 BE461961 BE461961 EST413299 tomato breaker fruit, TIG... 3 199 3.80E-12
G152 BE317746 BE317746 NF054H08LF1 F1064 Developing leaf Me... 1 201 3.90E-12
G152 AW039132 AW039132 EST281105 tomato mixed elicitor, BT... 1 199 3.90E-12
G152 AI487071 AI487071 EST245393 tomato ovary, TAMU Lycope... 1 199 3.90E-12
G152 BF317935 BF317935 OV1„9_F09.b1_A002 Ovary 1 (OV1 ) Sor... 3 199 3.90E-12
G152 BI272502 BI272502 NF023F04FL1F1042 Developing flower ... 1 200 3.90E-12
G152 AW331118 AW331118 707047D10.X1 707 - Mixed adult tiss... 3 198 3.90E-12
G152 AI488928 AI488928 EST247267 tomato ovary, TAMU Lycope... 2 199 3.90E-12
G152 BM324751 BM324751 PIC1_35_B11.b1_A002 Pathogen-infect... 1 200 4.00E-12
G152 BE918857 BE918857 FM1_1_A12.b1_A003 Floral-Induced Me... 1 199 4.00E-12
G152 BF113307 BF113307 EST440897 tomato breaker fruit Lyco... 3 199 4.00E-12
G152 BG048737 BG048737 OV1_22_D05.b1_A002 Ovary 1 (OV1) So... 3 200 4.10E-12
G152 BE918862 BE918862 FM1_1_B06.b1_A003 Floral-Induced Me... 1 199 4.10E-12
G152 AW617743 AW617743 EST324154 L. hirsutum trichome, Cor... 2 199 4.20E-12
IJJ LU LLI IJJ LU UJ UJ LU UJ UJ UJ UJ LU LU LIJ IJJ LU IJJ LU LU LU LU LU
O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O P P P P P
<λi cM CM CM w w M CM OT w n t m <o co r r' r ' r' rr r r r' r rr r rr r rr r r r r r r r r rr r' rr r
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Figure imgf000137_0001
rr rr s
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Figure imgf000137_0002
CM C CM CM CM CM CM Cλl CM CM CM CM C CM CM CM CM CM CM CM CM CM CM CM CΛI CλJ CM CM CM CM CM OJ CM CM CM CM m m m m m m m m m m m m m m m m m m m m m m m m m m m Ln in in LO Lo m δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ o co co co co c co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co
CM CM CM CM CM CM CM CM CM Lo-J IoJJ LoJJ IoJJ
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Figure imgf000138_0001
C0 C0 C0 ι- C0 0) C0 N C0 0> C0 C0 0) C0 O) (0 (D C0 N N C0 (0 C0 τ- C0 C0 N 05 C0 C0 ^ O O) O) O) i- τ- - ι- CM ι- τ- ι- ι- ι- r τ- τ- ι- ι- τ- τ- ι- τ- τ- t- - ι- ι- ι- ι- CV -- ι- ι- τ- r- - ϊ- CAI '- N
'- τ- C\l '- CO τ- τ- ι- ^ n « ι- W CM W CM ι- ι- ι- ι- CM ι- CO ι- C « N τ- (\j τ- C\I C τ- N CM n
Figure imgf000138_0002
CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM m m m m m m m m m m m m m m m m m m m m in m in LO LO LO Ln Lo in m m m m δδδδδδδδδδδδδ δδδδδδδδδδδδδδδδδδδδδδ
CO CO CO CO CO CO CO C CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO Table 2
G152 BE034098 BE034098 MG05B05 MG Mesembryanthemum crystal... 1 195 7.30E-12 1
G152 AF029977 AF029977 Eucalyptus grandis MADS box protein... 3 210 7.30E-12 1
G152 BE432640 BE432640 EST399169 tomato breaker fruit, TIG... 1 196 7.50E-12 1
G152 BE519003 BE519003946075F03.y1 946 - tassel primordiu... 3 196 7.90E-12 1
G152 BG441292 BG441292 GA Ea0012J14f Gossypium arboreum 7... 2 204 7.90E-12 1
G152 BF479063 BF479063 L48-2708T3 Ice plant Lambda Uni-Zap... 1 195 8.00E-12 1
G152 C75937 C75937 C75937 Rice callus Oryza sativa cDNA ... 2 198 8.10E-12 1
G152 BE474886 BE474886 sp69f12.y1 Gm-c1044 Glycine max cDN... 3 197 8.10E-12 1
G152 AL506480 AL506480 AL506480 Hordeum vulgare Barke deve... 1 195 8.10E-12 1
G152 AL506894 AL506894 AL506894 Hordeum vulgare Barke deve... 2 195 8.10E-12 1
G152 AL507763 AL507763 AL507763 Hordeum vulgare Barke deve... 1 195 8.10E-12 1
G152 AL508019 AL508019 AL508019 Hordeum vulgare Barke deve... 1 195 8.10E-12 1
G152 AL509053 AL509053 AL509053 Hordeum vulgare Barke deve... 2 195 8.10E-12 1
G152 AL506475 AL506475 AL506475 Hordeum vulgare Barke deve... 1 195 8.40E-12 1
G152 BI928852 BI928852 EST548741 tomato flower, 3 - 8 mm b... 1 196 8.50E-12 1
G152 BI405804 BI405804 146B05 Mature tuber lambda ZAP Sola... 1 195 8.80E-12 1
G152 C73941 C73941 C73941 Rice panicle (longer than 10cm... 1 196 8.90E-12 1
G152 OSY15008 Y15008 Oryza sativa mRNA for M79 protein. 10... 3 209 8.90E-12 1
G152 AW220779 AW220779 EST297248 tomato fruit mature green... 1 196 9.00E-12 1
G152 SAMADSD Y08626 S.alba mRNA for MADS D protein. 10/1997 1 209 9.20E-12 1
G152 AI486089 AI486089 EST244410 tomato ovary, TAMU Lycope... 3 195 9.30E-12 1
G152 BE354844 BE354844 EST354934 tomato flower buds 8 mm t... 3 196 9.40E-12 1
G152 BG837838 BG837838 Zm10_02b03_A Zm10_AAFC_ECORC_Fusari. -1 195 9.50E-12 1
G152 BG837866 BG837866 Zm10_03d05_A Zm10_AAFC_ECORC_Fusari. -1 195 9.50E-12 1
G152 AW290406 AW290406 NXNV019H03F Nsf Xylem Normal wood V... 1 196 9.60E-12 1
G152 BE462249 BE462249 EST324513 tomato flower buds 0-3 mm... 3 196 9.60E-12 1
G152 BG445047 BG445047 GA Ea0026l23f Gossypium arboreum 7... 3 202 9.60E-12 1
G152 BG318417 BG318417 NXPV_013_D07_F NXPV (Nsf Xylem Plan... 2 195 9.80E-12 1
G152 BE432051 BE432051 EST398580 tomato breaker fruit, TIG... 3 196 9.90E-12 1
G152 AB003324 AB003324 Oryza sativa mRNA for MADS box-like... 2 211 9.90E-12 1
G152 BG596731 BG596731 EST495409 cSTS Solanum tuberosum cD... 2 194 1.00E-11 1
G152 BG418810 BG418810 HVSMEk0024l17f Hordeum vulgare test... 2 203 1.00E-11 1
G152 BF728277 BF728277 1000060A05.X1 1000 - Unigene I from... 1 195 1.00E-11 1
G152 AW429227 AW429227 EST306683 tomato flower buds 0-3 mm... 1 195 1.00E-11 1
G152 BG605208 BG605208 WHE2330_A04_A08ZS Wheat pre-anthesi... 1 195 1.00E-11 1
G152 BE498620 BE498620 WHE0964_G04_M08ZS Wheat pre-anthesi... 2 195 1.00E-11 1
Table 2
G152 AV421179 AV421179 AV421179 Lotus japonicus young plan... 3 199 1.00E-11 1
G152 BE462244 BE462244 EST324508 tomato flower buds 0-3 mm... 3 196 1.00E-11 1
G152 AI897656 AI897656 EST267099 tomato ovary, TAMU Lycope... 1 195 1.00E-11 1
G152 ZMA292960 AJ292960 Zea mays mRNA for putative MADS-dom... 1 209 1.10E-11 1
G152 AF029976 AF029976 Eucalyptus grandis MADS box protein... 2 206 1.10E-11 1
G152 BG318835 BG318835 NXPV_020_D12_F NXPV (Nsf Xylem Plan... 1 195 1.10E-11 1
G152 AI484883 AI484883 EST243146 tomato ovary, TAMU Lycope... 1 194 1.10E-11 1
G152 BG367358 BG367358 HVSMEiOOI 1 P20f Hordeum vulgare 20 D... 2 195 1.10E-11 1
G152 C73896 C73896 C73896 Rice panicle (longer than 10cm... 3 196 1.10E-11 1
G152 AU029711 AU029711 AU029711 Rice panicle shorter than ... 3 196 1.10E-11 1
G152 AF335235 AF335235 Petunia x hybrida MADS-box transcri... 1 206 1.10E-11 1
G152 AF230704 AF230704 Petunia x hybrida MADS box transcri... 1 208 1.10E-11 1
G152 BE500707 BE500707 WHE0991-0994J21J21ZS Wheat pre-an... 3 195 1.20E-11 1
G152 AI485411 AI485411 EST243732 tomato ovary, TAMU Lycope... 1 194 1.20E-11 1
G152 PETTRNSFB M91666 Petunia transcription factor (fbp2) m... 3 207 1.20E-11 1
G152 SAU25696 U25696 Sinapis alba transcription factor SaM... 1 204 1.20E-11 1
G152 AW266670 AW266670 L0-1492T3 Ice plant Lambda Uni-Zap ... 1 195 1.20E-11 1
G152 MDAJ1681 AJ001681 Malus domestica mRNA for MADS box p... 3 211 1.20E-11 1
G152 BG274189 BG274189 WHE2230_F01_K02ZS Aegilops speltoid... 2 195 1.20E-11 1
G152 AF097746 AF097746 Cryptomeria japonica putative MADS-... 3 205 1.20E-11 1
G152 BF778113 BF778113 NXSI_077_C08_F NXSI (Nsf Xylem Side... 3 195 1.30E-11 1
G152 BE606178 BE606178 WHE0913_H09_O1 ZS Wheat 5-15 DAP sp... 1 195 1.30E-11 1
G152 BG625803 BG625803 NXPV_066_C06_F NXPV (Nsf Xylem Plan... 2 195 1.30E-11 1
G152 LESTDR5 X60480 L.esculentum TDR5 mRNA. 3/1992 2 205 1.30E-11 1
G152 LETDR5 X60758 L.esculentum TDR5 mRNA. 3/1992 2 205 1.30E-11 1
G152 AF180365 AF180365 Hieracium piloselloides DEFICIENS h... 1 193 1.30E-11 1
G152 MDAJ762 AJ000762 Malus domestica mRNA for MADS-box p... 3 203 1.40E-11 1
G152 AP004616 AP004616 Oryza sativa chromosome 6 clone P04... -2 215 1.40E-11 1
G152 BM500623 BM500623 PAC000000000051 Pioneer AF-1 array ... 3 195 1.40E-11 1
G152 BG444639 BG444639 GA Ea0025A08f Gossypium arboreum 7... 2 204 1.40E-11 1
G152 AI483797 AI483797 EST249668 tomato ovary, TAMU Lycope... 2 194 1.40E-11 1
G152 BG414586 BG414586 HVSMEk0002P16f Hordeum vulgare test... 3 195 1.40E-11 1
G152 AI771416 AI771416 EST252516 tomato ovary, TAMU Lycope... 1 197 1.40E-11 1
G152 AI895449 AI895449 EST264892 tomato callus, TAMU Lycop... 1 194 1.50E-11 1
G152 AL384006 AL384006 MtBC18C11 F1 MtBC Medicago truncatul... 3 194 1.50E-11 1
G152 BE471370 BE471370 EST416223 potato stolon, Cornell Un... 2 194 1.50E-11 1
Table 2
G152 BF276751 BF276751 GA Eb0030l08f Gossypium arboreum 7... 2 194 1.50E-11 1
G152 AW219962 AW219962 EST302445 tomato root during/after ... 2 193 1.50E-11 1
G152 BE445107 BE445107 WHE1132_G12_N24ZS Wheat etiolated s... 3 194 1.50E-11 1
G152 AW309024 AW309024 sf93h07.y1 Gm-c1019 Glycine max cDN... 3 193 1.50E-11 1
G152 BG241308 BG241308 OV1_40_G05.b1_A002 Ovary 1 (OV1) So... 2 194 1.50E-11 1
G152 BF482642 BF482642 WHE2301-2304_A17_A17ZS Wheat pre-an... 2 195 1.50E-11 1
G152 BI434909 BI434909 EST537774 P. infestans-challenged I... 2 192 1.50E-11 1
G152 BG417111 BG417111 HVSMEk0016F04f Hordeum vulgare test... 3 194 1.50E-11 1
G152 BG240086 BG240086 OV1_17_G05.b1_A002 Ovary 1 (OV1) So... 2 194 1.50E-11 1
G152 BG417142 BG417142 HVSMEk0016H10f Hordeum vulgare test... 1 194 1.50E-11 1
G152 AI781869 AI781869 EST262748 tomato susceptible, Come... 2 193 1.50E-11 1
G152 AF099937 AF099937 Hyacinthus orientalis AGAMOUS homol... 2 208 1.60E-11 1
G152 AF335244 AF335244 Petunia x hybrida MADS-box transcri... 1 200 1.60E-11 1
G152 BI934464 BI934464 EST554353 tomato flower, anthesis L... 3 192 1.60E-11 1
G152 AF068722 AF068722 Nicotiana sylvestris MADS-box prote... 3 207 1.60E-11 1
G152 AF335241 AF335241 Petunia x hybrida MADS-box transcri... 1 206 1.70E-11 1
G152 BI929568 BI929568 EST549457 tomato flower, 3 - 8 mm b... 2 192 1.70E-11 1
G152 AW738070 AW738070 EST339497 tomato flower buds, anthe... 1 192 1.70E-11 1
G152 AI895327 AI895327 EST264770 tomato callus, TAMU Lycop... 1 194 1.70E-11 1
G152 AC109595 AC109595 Oryza sativa chromosome 5 clone OJ1... 3 214 1.80E-11 1
G152 AF209729 AF209729 Hemerocallis hybrid cultivar putati... 1 199 1.80E-11 1
G152 BE637039 BE637039 WHE1821-1824J01J01ZS Secale cerea... 2 194 1.80E-11 1
G152 BE055946 BE055946 945020E05.Y1 945 - Mixed adult tiss... 1 193 1.90E-11 1
G152 AI898947 AI898947 EST268390 tomato ovary, TAMU Lycope... 3 193 1.90E-11 1
G152 BI957545 BI957545 HVSMEn0010B09f Hordeum vulgare rach... 3 192 1.90E-11 1
G152 AW278878 AW278878 sf99h09.y1 Gm-c1019 Glycine max cDN... 3 192 2.00E-11 1
G152 BI928804 BI928804 EST548693 tomato flower, 3 - 8 mm b... 1 192 2.00E-11 1
G152 BCA251300 AJ251300 Brassica rapa subsp. pekinensis mRN... 1 191 2.00E-11 1
G152 BNADBDA L36927 Brassica oleracea (subspecies botryti... 1 191 2.00E-11 1
G152 BNADBD L36926 Brassica oleracea (clone pBS85) BoCAL... 1 191 2.00E-11 1
G152 AW62471 AW624717 EST322662 tomato flower buds 3-8 mm... 3 192 2.00E-11 1
G152 BG626111 BG626111 cC-esflcLEL10P07a1 Tomato flower Ii... 3 194 2.00E-11 1
G152 AF023615 AF023615 Pinus radiata MADS box protein mRNA... 2 204 2.00E-11 1
G152 AW622620 AW622620 EST313420 tomato root during/after ... 1 192 2.00E-11 1
G152 AW624642 AW624642 EST322587 tomato flower buds 3-8 mm... 1 192 2.10E-11 1
G152 BM732175 BM732175 sal74a08.y1 Gm-c1061 Glycine max cD... 3 192 2.10E-11 1
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CM CM CM I CM CM CM CM CM CM CM CM OJ OJ CM CM CM C CM OJ CM CM CM CM CM CM CM CM CM CM OI OJ CM CM CM CM m m m m m m m m m m m m m m m m m m m m m m in LO Lo m m m m Ln ooooooooδδδδδδδδδδδδδδδδδδδδδ δδδδδδ co co co co co co co c co co co co co co co co co co co co co co co co co co co co co c co co co co co co Table 2
G152 BE194007 BE194007 HVSMEh0083F13f Hordeum vulgare 5-45... 2 189 4.40E-11
G152 C74564 C74564 C74564 Rice panicle shorter than 3cm ... 2 190 4.50E-11
G152 PRU90349 U90349 Pinus radiata putative MADS box trans... 2 188 4.50E-11
G152 GGN132209 AJ132209 Gnetum gnemon mRNA for putative MAD... 1 205 4.50E-11
G152 BI924402 BI924402 EST544291 tomato flower, buds 0-3 m... 3 189 4.50E-11
G152 PRU42400 U42400 Pinus radiata putative MADS-box famil... 2 204 4.50E-11
G152 AF158544 AF158544 Picea abies clone DAL13-2 MADS-box ... 3 203 4.50E-11
G152 AW277299 AW277299 sf80e05.y1 Gm-c1019 Glycine max cDN... 3 189 4.50E-11
G152 GI-9964296 AF112149_1 MADS box protein 2 [Zea mays] 234 9.80E-47 5
G152 GI-1816459 DEFH125 protein 244 4.60E-42 5
G152 GI-7489303 T17029 MADS-box transcription factor DEFH1... 244 4.60E-42 5
G152 GI-15081463 AF396746_1 MADS-box protein [Ipomoea batatas] 198 7.40E-40 7
G152 GI-1928874 MADS-box protein 212 2.00E-35 5
G152 GI-7488744 T09700 MADS-box protein - alfalfa (fragment) 212 2.00E-35 5
G152 GI-18996775 AF462152_1 MADS-box transcription factor H... 204 1.10E-24 3
G152 GI-4103342 agamous-like putative transcription factor 199 1.90E-24 4
G152 GI-951172 MADS box protein 202 3.70E-24 4
G152 GI-1001934 ZAG2 202 3.70E-24 4
G152 GI-2997615 CUM10 199 3.80E-24 4
G152 GI-7446521 T08040 MADS-box protein - cucumber 199 3.80E-24 4
G152 GI-602906 SLM4 202 4.50E-24 3
G152 GI-1001935 ZMM1 201 4.80E-24 4
G152 GI-1167914 MADS box protein 201 4.80E-24 4
G152 GI-7446525 T02261 MADS box protein - maize 201 4.80E-24 4
G152 GI-4218162 MADS-box protein, GAGA2 199 4.80E-24 4
G152 GI-167126 BAG1 194 6.30E-24 4
G152 GI-322650 A43484 probable transcription factor BAG1 ... 194 6.30E-24 4
G152 GI-399096 AG_BRANA AGAMOUS PROTEIN 194 6.30E-24 4
G152 GI-10946429 AF305076_1 MADS-box protein EAP1 [Eucalypt... 184 6.70E-24 4
G152 GI-4204232 MADS-box protein 1 201 7.30E-24 3
G152 GI-3646320 MADS-box protein 195 7.50E-24 3
G152 GI-3688589 MADS box transcription factor 198 1.90E-23 3
G152 GI-11037010 AF305696_1 MADS-box protein EAP2S [Eucalyp... 184 2.00E-23 3
G152 GI-11120557 AF306349_1 MADS box protein AP2L [Eucalypt... 184 2.00E-23 3
G152 GI-7592642 API-like MADS box protein [Oryza sativa] 202 6.10E-23 3
G152 GI-6469345 DNA-binding protein 191 6.50E-23 3
Table 2
G152 GI-3947985 MADS-box protein 2 189 8.30E-23 3
G152 GI-642591 amino acid feature: K-box, bp 283..480; am... 191 1.30E-22 3
G152 GI-1561784 homeotic protein boiCAL 191 1.30E-22 3
G152 GI-7446539 T14456 MADS box protein homolog CAL - wild... 191 1.30E-22 3
G152 GI-6634708 AF176782_1 MADS box transcription factor 188 1.30E-22 3
G152 GI-6731756 unnamed protein product 188 1.30E-22 3
G152 GI-5070144 AF068726_1 MADS-box protein MADS2 193 1.70E-22 3
G152 GI-12019658 FDRMADS6 [Oryza sativa] 200 2.00E-22 3
G152 GI-19382 TDR4 189 2.10E-22 3
G152 GI-542035 S23730 TDR4 protein - tomato 189 2.10E-22 3
G152 GI-3912999 AGL8J.YCES FLORAL HOMEOTIC PROTEIN AGL8 HO... 189 2.10E-22 3
G152 GI-4102113 NAP1-2 194 2.70E-22 3
G152 G1-5070140 AF068724_1 MADS-box protein MADS5 190 2.70E-22 3
G152 GI-609253 Saapl 189 2.70E-22 3
G152 GI-1076477 S52236 Saapl protein - white mustard 189 2.70E-22 3
G152 GI-3913047 AP1_SINAL FLORAL HOMEOTIC PROTEIN APETALA1... 189 2.70E-22 3
G152 GI-16973298 C-type MADS box protein [Malus x domestica] 204 3.20E-22 3
G152 GI-4101710 MADS box transcription factor - 204 3.20E-22 3
G152 GI-6970411 MADS-box protein 198 3.30E-22 3
G152 GI-13384068 AF335245_1 MADS-box transcription factor F... 195 3.30E-22 3
G152 GI-14518447 AF130118_1 MADS box protein [Capsicum annuum] 187 3.50E-22 3
G152 GI-4103486 MADS box protein 204 4.10E-22 3
G152 GI-695688 DAL2 protein 204 4.10E-22 3
G152 G1-4033710 AGAMOUS-like MADS-box transcription factor... 204 4.10E-22 3
G152 GI-4033721 AGAMOUS-like MADS-box transcriptional fact... 204 4.10E-22 3
G152 GI-4033723 AGAMOUS-like MADS-box transcriptional fact... 204 4.10E-22 3
G152 GI-7446536 T14847 MADS-box protein dal2 - Norway spruce 204 4.10E-22 3
G152 GI-14279306 AF265562_1 MAD-box transcripion factor [V . 202 5.20E-22 3
G152 GI-758565 transcription factor 189 5.50E-22 3
G152 GI-758567 transcription factor 189 5.50E-22 3
G152 GI-3913001 AGL8_SOLTU FLORAL HOMEOTIC PROTEIN AGL8 HO... 189 5.50E-22 3
G152 GI-7446508 T07100 MADS box protein homolog POTM1 -1 - ... 189 5.50E-22 3
G152 GI-4103757 MADS1 201 6.60E-22 3
G152 GI-6606306 AF176783_1 floral binding protein 26 183 7.20E-22 3
G152 GI-6606070 AF058697_1 MADS14 protein 194 8.70E-22 3
G152 GI-457382 TAG1 203 1.10E-21 3
Table 2
G152 GI-3913004 AGJ-YCES AGAMOUS PROTEIN (TAG1) 1 203 1.10E-21 3
G152 GI-7446522 T07185 floral homeotic protein TAG1 - tomato 1 203 1.10E-21 3
G152 GI-5070142 AF068725_1 MADS-box protein MADS1 1 180 1.20E-21 3
G152 GI-2290778 MADS box transcription factor 1 189 1.40E-21 3
G152 GI-3912987 AGL8_SOLCO FLORAL HOMEOTIC PROTEIN AGL8 HO. 1 189 1.40E-21 3
G152 GI-7446507 T07902 MADS box protein - Commerson's wild... 1 189 1.40E-21 3
G152 GI-16549058 putative MADS-domain transcription factor ... 1 215 1.80E-21 2
G152 GI-9367309 MADS-box protein 5 [Hordeum vulgare] 1 179 1.90E-21 3
G152 GI-4218160 MADS-box protein, GAGA1 1 199 2.20E-21 3
G152 GI-15808691 AF185574_1 transcription factor MAGL4 [Pop... 1 198 2.20E-21 3
G152 GI-16874557 MADS-box transcription factor DEFH28 [Anti... 1 192 2.30E-21 3
G152 GI-2981133 AGAMOUS homolog 1 197 2.80E-21 3
G152 GI-9964074 MADS-box transcription factor jointless [L... 1 217 2.80E-21 2
G152 GI-17433048 JOINJ-YCES MADS-box JOINTLESS protein (LeM... 1 217 2.80E-21 2
G152 GI-4218175 MADS-box protein, GSQUA1 1 188 2.90E-21 3
G152 GI-12655901 AF226865_1 putative transcription factor A... 1 196 3.60E-21 3
G152 GI-17223670 SHATTERPROOF1 [Brassica napus] 1 196 3.60E-21 3
G152 GI-4102111 NAP1-1 1 186 3.70E-21 3
G152 GI-13446154 MADS-box transcription factor [Pisum sativum] 1 193 4.60E-21 3
G152 GI-13661024 MADS-box transcription factor [Pisum sativum] 1 193 4.60E-21 3
G152 GI-18252655 AF461740_1 MADS-box transcription factor M... 1 193 4.60E-21 3
G152 GI-13448660 MADS box transcription factor [Ipomoea bat... 1 211 4.70E-21 2
G152 GI-5019431 putative MADS domain transcription factor ... 1 205 5.50E-21 3
G152 GI-6683777 AF135962_1 CAGL2 1 202 5.60E-21 3
G152 GI-16973296 C-type MADS box protein [Malus x domestica] 1 198 5.70E-21 3
G152 GI-12597207 putative MADS-box protein [Cucumis sativus] 1 198 5.70E-21 3
G152 GI-2735764 MADS transcriptional factor; STMADS16 1 213 5.90E-21 2
G152 GI-7446547 T06995 probable MADS box transcription fac... 1 213 5.90E-21 2
G152 GI-3290209 MADS-box protein 1 1 211 6.00E-21 2
G152 GI-3646334 MdMADS8 1 211 6.00E-21 2
G152 GI-7488622 T17023 MADS box protein 1 - apple tree 1 211 6.00E-21 2
G152 GI-6467974 AF198175_1 MADS box protein DOMADS2 1 200 6.20E-21 2
G152 GI-6580947 AF158543_1 MADS-box transcription factor 1 203 7.00E-21 3
G152 GI-6467976 AF198176_1 MADS box protein DOMADS3 1 199 7.10E-21 3
G152 GI-6092011 GpMADS4 1 221 7.40E-21 2
G152 GI-4837612 farinelli protein 1 192 7.40E-21 3
Table 2
G152 GI-13384052 AF335237_1 MADS-box transcription factor F... 1 208 7.70E-21 2
G152 GI-431736 NAG1 1 199 9.00E-21 3
G152 GI-3913007 AG_TOBAC AGAMOUS PROTEIN (NAG1) 1 199 9.00E-21 3
G152 GI-7446523 T03592 floral homeotic protein NAG1 - comm... 1 199 9.00E-21 3
G152 GI-13384046 AF335234_1 MADS-box transcription factor F... 1 214 9.60E-21 2
G152 GI-13448658 MADS box transcription factor [Ipomoea bat... 1 213 9.60E-21 2
G152 GI-1905944 MADS box transcription factor SbMADSI 1 205 9.90E-21 2
G152 GI-7446545 T14801 MADS box protein MADS1 - sorghum 1 205 9.90E-21 2
G152 GI-320596 JQ1690 floral binding protein 2 - garden p... 1 207 1.20E-20 2
G152 GI-1181186 transcription factor 1 207 1.20E-20 2
G152 GI-1345965 FBP2_PETHY FLORAL HOMEOTIC PROTEIN FBP2 (F... 1 207 1.20E-20 2
G152 GI-5070138 AF068722_1 MADS-box protein MADS3 1 207 1.20E-20 2
G152 GI-1905930 MADS box protein 1 206 1.20E-20 2
G152 GI-7446519 T04335 MADS box protein - rice 1 206 1.20E-20 2
G152 GI-13384058 AF335240_1 MADS-box transcription factor F... 1 199 1.30E-20 2
G152 GI-13384060 AF335241_1 MADS-box transcription factor F... 1 206 1.60E-20 2
G152 GI-1206005 putative MADS-box family transcription factor 1 204 1.60E-20 2
G152 GI-1702951 MADS-box family transcription factor 1 204 1.60E-20 2
G152 GI-7446546 T10486 MADS box protein - Canadian red pine 1 204 1.60E-20 2
G152 GI-7446559 T09571 MADS box protein MADS2 - Monterey pine 1 204 1.60E-20 2
G152 GI-6580943 AF158541_1 MADS-box transcription factor 1 198 1.60E-20 2
G152 GI-6580945 AF158542_1 MADS-box transcription factor 1 198 1.60E-20 2
G152 GI-6006607 MADS box protein, MADS28 1 193 1.70E-20 2
G152 GI-13810204 MADS1 protein [Cucumis sativus] 1 201 1.80E-20 3
G152 GI-1206003 putative MADS-box family transcription factor 1 210 2.00E-20 2
G152 GI-7446558 T09569 MADS box protein MADS1 - Monterey pine 1 210 2.00E-20 2
G152 GI-5019456 putative MADS domain transcription factor ... 1 206 2.00E-20 2
G152 GI-6092009 GpMADS3 1 206 2.00E-20 2
G152 GI-695686 dal1 1 206 2.00E-20 2
G152 GI-2160701 MADS-box protein 1 206 2.00E-20 2
G152 GI-7446537 T14846 probable MADS-box protein dall - No... 1 206 2.00E-20 2
G152 GI-7446551 T09603 MADS-box protein 3 - Monterey pine 1 206 2.00E-20 2
G152 GI-19358 TDR5 1 205 2.00E-20 2
G152 GI-19384 TDR5 1 205 2.00E-20 2
G152 GI-629674 S23728 TDR5 protein - tomato 1 205 2.00E-20 2
G152 GI-1239959 MADS-box gene 1 205 2.00E-20 2
Table 2
G152 GI-3913002 AGL9_LYCES FLORAL HOMEOTIC PROTEIN AGL9 HO... 205 2.00E-20 2
G152 GI-7446511 S71757 MADS box protein DEFH200 - garden s... 205 2.00E-20 2
G152 GI-2286113 MADS box protein 204 2.00E-20 2
G152 GI-7446544 T04170 MADS box protein - rice 204 2.00E-20 2
G152 GI-9367315 MADS-box protein 9 [Hordeum vulgare] 203 2.00E-20 2
G152 GI-4096982 AGAMOUS protein 201 2.30E-20 3
G152 GI-6970413 MADS-box protein 201 2.30E-20 3
G152 GI-6970417 MADS-box protein 201 2.30E-20 3
G152 GI-2463333 M79 protein 209 2.50E-20 2
G152 GI-7446518 T04307 M79 protein - rice 209 2.50E-20 2
G152 GI-3114584 MADS box protein 204 2.60E-20 2
G152 GI-3646340 MADS-box protein 201 2.60E-20 2
G152 GI-6840998 AF120097_1 DEF/GLO-like protein 202 2.90E-20 3
G152 GI-313113 pMADS3 199 3.00E-20 3
G152 GI-478387 JQ2212 pMADS3 protein - garden petunia 199 3.00E-20 3
G152 GI-3913006 AG_PETHY AGAMOUS PROTEIN 199 3.00E-20 3
G152 GI-17827467 PMADS3 [Petunia x hybrida] 199 3.00E-20 3
G152 GI-1321797 putative transcription factor 197 3.00E-20 3
G152 GI-4103344 agamous-like putative transcription factor 197 3.00E-20 3
G152 GI-7446562 T10185 MADS-box protein CUS1 - cucumber 197 3.00E-20 3
G152 GI-1617211 MADS D 209 3.20E-20 2
G152 GI-3912986 AGL9_SINAL FLORAL HOMEOTIC PROTEIN AGL9 HO... 209 3.20E-20 2
G152 GI-7446553 T10467 MADS box protein D - white mustard 209 3.20E-20 2
G152 GI-2286111 MADS box protein 202 3.30E-20 2
G152 GI-7446543 T04169 MADS box protein - rice 202 3.30E-20 2
G152 GI-6175371 AF091458_1 MADS box transcription factor M... 193 3.40E-20 2
G152 GI-5295978 MADS box-like protein 183 4.00E-20 3
G152 GI-1239963 MADS-box gene 202 4.20E-20 2
G152 GI-7446510 S71756 MADS box protein DEFH72 - garden sn... 202 4.20E-20 2
G152 GI-939779 MADS box protein 200 4.20E-20 2
G152 GI-7446514 T03398 MADS box protein - maize 200 4.20E-20 2
G152 GI-13958339 AF372840_1 madsl [Poa annua] 200 4.20E-20 2
G152 GI-1483230 MADS4 protein 197 4.30E-20 2
G152 GI-6580949 AF158544_1 MADS-box transcription factor 203 4.70E-20 3
G152 GI-8574457 AF072534_1 pepper MADS-box protein [Capsic... 211 5.20E-20 2
G152 GI-790637 AGL15 209 5.20E-20 2
Table 2
G152 GI-3831486 AG15_BRANA FLORAL HOMEOTIC PROTEIN AGL15 1 209 5.20E-20 2 G152 GI-7446560 T07867 MADS box protein AGL15 (type 1) - rape 1 209 5.20E-20 2 G152 GI-695317 MADS box protein 1 203 5.30E-20 2 G152 GI-3023536 CMB1_DIACA MADS BOX PROTEIN CMB1 1 203 5.30E-20 2 G152 GI-7446563 T10714 MADS-box protein CMB1 - clove pink 1 203 5.30E-20 2 G152 GI-16549085 putative MADS-domain transcription factor ... 1 192 5.50E-20 2 G152 GI-13384048 AF335235_1 MADS-box transcription factor F... 1 206 6.70E-20 2 G152 GI-3646324 MADS-box protein 1 200 6.80E-20 2 G152 GI-5777904 U78949_1 MADS-box protein 3 1 200 6.80E-20 2 G152 GI-3688591 MADS box transcription factor 1 200 6.80E-20 2 G152 GI-508577 box protein 1 198 6.90E-20 2 G152 GI-1076739 S53306 box protein - rice 1 198 6.90E-20 2 G152 GI-10188329 unnamed protein product [Zea mays] 1 198 6.90E-20 2 G152 GI-11493807 MADS box protein MADS1 [Oryza sativa] 1 198 6.90E-20 2 G152 GI-7544096 pMADS4 [Petunia x hybrida] 1 197 6.90E-20 2 G152 GI-6468286 putative MADS domain transcription factor ... 1 194 7.80E-20 3 G152 GI-695690 DAL3 protein 1 194 7.80E-20 3 G152 GI-7484434 T14848 MADS-box protein dal3 - Norway spruce 1 194 7.80E-20 3 G152 GI-4033725 AGAMOUS-like MADS-box transcriptional fact... 1 182 8.30E-20 3 G152 GI-3646326 MADS-box protein 1 203 8.60E-20 2 G152 GI-6651033 AF129875_1 MADS box transcription factor M... 1 203 8.60E-20 2 G152 GI-5019460 putative MADS domain transcription factor ... 1 202 8.60E-20 2 G152 GI-2286109 MADS box protein 1 200 8.70E-20 2 G152 GI-6650550 AF109153_1 MADS box transcription factor M... 1 200 8.70E-20 2 G152 GI-7446517 T04167 MADS box protein - rice 1 200 8.70E-20 2 G152 GI-5019464 putative MADS domain transcription factor ... 1 202 9.50E-20 3 G152 GI-13384066 AF335244 MADS-box transcription factor F... 1 200 1.10E-19 2 G152 GI-939781 MADS box protein 1 199 1.10E-19 2 G152 GI-7446515 T03408 MADS box protein - maize 1 199 1.10E-19 2 G152 GI-15824795 AF345911_1 MADS-box protein FDRMADS3 [Oryz... 1 196 1.10E-19 2 G152 GI-3986689 MADS box protein 1 210 1.40E-19 2 G152 GI-6652756 MADS box protein 1 207 1.40E-19 2 G152 GI-1049022 transcription factor SaMADS A 1 204 1.40E-19 2 G152 GI-7446552 T10422 MADS box protein A - white mustard 1 204 1.40E-19 2 G152 GI-11494137 AF209729_1 putative MADS box transcription... 1 199 1.40E-19 2 G152 GI-642593 amino acid feature: MADS box; codes for a ... 1 191 1.40E-19 2
Table 2
13 G152 GI-7488599 T14457 MADS box protein homolog CAL - broc... 191 1.40E-19 2
13 G152 GI-1239961 MADS-box gene 205 1.80E-19 2
13 G152 GI-7446512 S78015 MADS box protein DEFH49 - garden sn... 205 1.80E-19 2
13 G152 GI-4433623 MADS-box transcription factor 204 1.80E-19 2
13 G152 GI-6467972 AF198174_1 MADS box protein DOMADS1 204 1.80E-19 2
13. G152 GI-16052 SQUA 198 1.80E-19 2
13 G152 GI-82313 S20886 transcription factor squa - garden ... 198 1.80E-19 2
13 G152 GI-13384050 AF335236_1 MADS-box transcription factor F... 198 1.80E-19 2
13 G152 GI-9367311 MADS-box protein 7 [Hordeum vulgare] 197 1.80E-19 2
13 G152 GI-886405 MADS box protein 203 1.90E-19 3
13 G152 GI-2130078 S59480 MADS-box protein 3 - rice 203 1.90E-19 3
13 G152 GI-13161415 putative MADS-box protein [Oryza sativa] 203 1.90E-19 3
13 G152 GI-18650789 AF234617_1 MADS-box transcription factor [... 202 1.90E-19 3
13 G152 GI-264223 promotes sex organ development=ple [Antirr... 193 2.00E-19 3
13 G152 GI-322801 A44343 promotes sex organ development prot... 193 2.00E-19 3
13 G152 GI-1483232 MADS5 protein 189 2.40E-19 2
13 G152 GI-8216957 putative transcription factor [Cucumis sat... 182 2.70E-19 3
13 G152 GI-5295982 MADS box-like protein 211 2.80E-19 2
13 G152 GI-8163950 AF230704_1 MADS box transcription factor T... 208 2.80E-19 2
13 G152 GI-439239 MADS-box protein 199 2.90E-19 2
13 G152 GI-632112 S40405 MADS-box protein - Aranda deborah 199 2.90E-19 2
13 G152 GI-3912996 AGL9_ARADE FLORAL HOMEOTIC PROTEIN AGL9 HO... 199 2.90E-19 2
13 G152 GI-1914838 MADS box protein 196 2.90E-19 2
13 G152 GI-5295964 EST D15657(C1032) corresponds to a region... 196 2.90E-19 2
13 G152 GI-7446542 T04168 MADS box protein - rice 196 2.90E-19 2
13 . G152 GI-5051933 MADS-box protein FDRMADS8 193 3.00E-19 2 3 G152 GI-602908 SLM5 186 3.40E-19 3 3 G152 GI-3114588 MADS box protein 210 3.60E-19 2
13 G152 GI-5777906 U78950_1 MADS-box protein 4 200 3.70E-19 2
13 G152 GI-4322475 putative MADS box transcription factor ETL 198 3.70E-19 2
13 G152 GI-10188331 unnamed protein product [Zea mays] 198 3.70E-19 2
13 G152 GI-4204234 MADS-box protein 2 194 3.70E-19 2
13 G152 GI-18057092 AC092697_3 putative transcription factor [... 193 3.80E-19 2
13 G152 GI-13274182 putative MADS-domain transcription factor ... 213 4.50E-19 2
13 G152 GI-8745070 MADS box protein [Betula pendula] 197 4.70E-19 2
13 G152 GI-1418272 AGL15type2 196 4.70E-19 2
Table 2
13 G152 GI-7446561 T07869 MADS box protein AGL15 (type 2) - rape 1 196 4.70E-19 2
13 G152 GI-1568513 fbp11 1 197 5.10E-19 3
13 G152 GI-5031217 AGAMOUS homolog 1 203 5.90E-19 2
13 G152 GI-18076209 putative MADS-domain transcription factor ... 1 203 5.90E-19 2
13 G152 GI-8745072 MADS box protein [Betula pendula] 1 201 5.90E-19 2
13 G152 GI-2293894 unnamed protein product 1 189 6.10E-19 2
13 G152 GI-2997613 CUM1 1 202 6.30E-19 3
13 G152 GI-4103346 agamous-like putative transcription factor 1 202 6.30E-19 3
13 G152 GI-7446520 T08039 MADS-box protein - cucumber 1 202 6.30E-19 3
13 G152 GI-9994504 FBP15 [Petunia x hybrida] 1 202 6.30E-19 3
13 G152 GI-12002141 AF112150_1 MADS box protein 3 [Zea mays] 1 197 7.00E-19 2
13 G152 GI-3170502 APETALA3 homolog PnAP3-2 1 200 7.50E-19 2
13 G152 GI-431908 transcription factor 1 198 7.60E-19 2
13 G152 GI-1076646 S46526 transcription factor - common tobacco 1 198 7.60E-19 2
13 G152 GI-1049024 transcription factor SaMADS B 1 193 7.70E-19 2
13 G152 GI-3913000 AGL8_SINAL FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 193 7.70E-19 2
13 G152 GI-13177638 AF265554_1 transcription factor CMB [Cucum... 1 201 8.00E-19 3
13 G152 GI-848999 agamous protein 1 197 9.70E-19 2
Ul
© 13 G152 GI-1483228 MADS3 protein 1 189 9.90E-19 2
13 G152 GI-3646336 MdMADSΘ 1 184 1.00E-18 2
13 G152 GI-6470126 AF151693_1 transcription factor 1 200 1.20E-18 2
13 G152 GI-2981131 AGAMOUS homolog 1 196 1.20E-18 2
13 G152 GI-13274178 putative MADS-domain transcription factor ... 1 211 1.50E-18 2
13 G152 GI-13274180 putative MADS-domain transcription factor ... 1 209 1.50E-18 2
13 G152 GI-16973294 B-type MADS box protein [Malus x domestica] 1 190 1.60E-18 2
13 G152 GI-886401 MADS box protein 1 211 1.90E-18 2
13 G152 GI-7446534 T03894 MADS box protein - rice 1 211 1.90E-18 2
13 G152 GI-13272279 MADS [Oryza sativa] 1 211 1.90E-18 2
13 G152 GI-6970415 MADS-box protein 1 202 2.00E-18 2
13 G152 GI-887579 MADS box regulatory protein 1 198 2.00E-18 2
13 G152 GI-1046276 MADS box regulatory protein 1 198 2.00E-18 2
13 G152 GI-1364102 S57586 MADS box regulatory protein - Rumex... 1 198 2.00E-18 2
13 G152 GI-13384056 AF335239_1 MADS-box transcription factor F... 1 198 2.00E-18 2
13 G152 GI-3493647 transcription activator 1 197 2.00E-18 2
13 G152 GI-4105097 MADS box protein 26 1 196 2.00E-18 2
13 G152 GI-1561780 homeotic protein boι'2AP1 1 188 2.00E-18 2
Table 2
G152 GI-4416347 MADS C-2 protein; MADS-box protein 1 188 2.00E-18 2 G152 GI-10835358 PTD protein [Populus balsamifera subsp. tr... 1 200 2.50E-18 2 G152 GI-396199 fbp6 1 197 2.50E-18 2 G152 GI-2129972 S60307 fbp6 protein - garden petunia 1 197 2.50E-18 2 G152 GI-16549070 putative MADS-domain transcription factor ... 1 196 2.50E-18 2 G152 GI-7672991 AF144623_1 MADS-box transcription factor [... 1 199 3.20E-18 2 G152 GI-861081 agamous 1 198 4.10E-18 2 G152 GI-3913005 AG_PANGI AGAMOUS PROTEIN (GAG2) 1 198 4.10E-18 2 G152 GI-3688593 MADS box transcription factor 1 198 4.10E-18 2 G152 GI-5295980 MADS box-like protein 1 196 5.20E-18 2 G152 GI-5051935 MADS-box protein FDRMADS1 1 181 5.50E-18 2 G152 GI-9367307 MADS-box protein 3 [Hordeum vulgare] 1 191 6.20E-18 2 G152 GI-19380 TDR3 1 199 6.60E-18 2 G152 GI-542034 S23729 TDR3 protein - tomato 1 199 6.60E-18 2 G152 GI-3337237 putative MADS box transcription factor PrM... 1 188 6.80E-18 2 G152 GI-7446557 T10778 probable MADS box protein MADS8 - M... 1 188 6.80E-18 2 G152 GI-3170464 APETALA3 homolog PnPI-1 1 202 8.30E-18 2 G152 GI-2981612 transcription factor 1 216 9.00E-18 2 G152 GI-1561778 homeotic protein boil AP1 1 187 1.00E-17 2 G152 GI-4406132 MADS box protein 1 193 1.10E-17 2 G152 GI-16417764 AF424549_1 MADS-box protein SPW1 [Oryza sa... 1 193 1.10E-17 2 G152 GI-12964064 MADS box transcription factor [Oryza sativa] 1 189 1.10E-17 2 G152 GI-2507629 putative MADS box transcription factor PrM... 1 187 1.10E-17 2 G152 GI-7446556 T10776 probable MADS box protein MADS6 - M... 1 187 1.10E-17 2 G152 GI-9367313 MADS-box protein 8 [Hordeum vulgare] 1 195 1.30E-17 2 G152 GI-2252482 putative MADS domain transcription factor 1 212 1.30E-17 2 G152 GI-9956938 AF150931_1 MADS-domain protein PPM1 [Physc... 1 229 1.30E-17 1 G152 GI-9956940 AF150932_1 MADS-domain protein PPM1 [Physc... 1 229 1.30E-17 1 G152 GI-3646322 MADS-box protein 1 191 1.40E-17 2 G152 GI-887392 BOAP1 1 186 1.40E-17 2 G152 GI-4887235 AGAMOUS homolog transcription factor 1 208 1.70E-17 2 G152 GI-22667 PMADS2 1 200 2.20E-17 2 G152 GI-322775 S31707 transcription factor pmads2 - garde... 1 200 2.20E-17 2 G152 GI-454265 FBP3 1 200 2.20E-17 2 G152 GI-729976 MAD2_PETHY FLORAL HOMEOTIC PROTEIN PMADS 2 1 200 2.20E-17 2 G152 GI-2129971 S60288 FBP3 protein - garden petunia 1 200 2.20E-17 2
Table 2
13 G152 GI-9956942 AF150933_1 MADS-domain protein PPM2 [Physc... 1 227 2.20E-17 1
13 G152 GI-9956944 AF150934_1 MADS-domain protein PPM2 [Physc... 1 227 2.20E-17 1
13 G152 GI-7328575 AF181479_1 MADS-box DNA binding protein [Z... 1 196 2.20E-17 2
13 G152 GI-1870206 MADS-box protein 1 189 2.20E-17 2
13 G152 GI-2507625 putative MADS box transcription factor PrM... 1 182 2.30E-17 2
13 G152 GI-7446564 T10764 probable MADS box protein MADS4 - M... 1 182 2.30E-17 2
13 G152 GI-5616513 AF168468_1 agamous protein 1 202 2.70E-17 2
13 G152 GI-19388 TDR8 1 200 2.70E-17 2
13 G152 GI-542036 S23732 TDR8 protein - tomato 1 200 2.70E-17 2
13 G152 GI-2507623 putative MADS box transcription factor PrM... 1 195 3.60E-17 2
13 G152 GI-7446554 T10751 MADS-box protein MADS9 - Monterey pine 1 195 3.60E-17 2
13 G152 GI-2827300 MADS-box protein NMH 7 1 192 3.60E-17 2
13 G152 GI-7446532 T09335 MADS-box protein NMH 7 - alfalfa 1 192 3.60E-17 2
13 G152 GI-13384054 AF335238_1 MADS-box transcription factor F... 1 186 3.70E-1 2
13 G152 GI-13442962 AF095646_1 MADS box protein nmads3 [Oryza ... 1 175 3.80E-17 2
13 G152 GI-13384062 AF335242_1 MADS-box transcription factor F... 1 198 4.50E-17 2
13 G152 GI-2735766 MADS transcriptional factor; STMADS11 1 192 4.60E-17 2
13 G152 GI-7446550 T06996 MADS-box transcription factor MADS1... 1 192 4.60E-17 2
Ul
13 G152 GI-2981614 transcription factor 1 191 4.60E-17 2
13 G152 GI-16549081 putative MADS-domain transcription factor ... 1 173 4.80E-17 2
13 G152 GI-2529340 homologue; putative 1 198 5.70E-17 2
13 G152 GI-7446524 T01700 hypothetical protein - maize 1 198 5.70E-17 2
13 G152 GI-5295984 MADS box-like protein 1 192 6.80E-17 2
13 G152 GI-939785 MADS box protein 1 194 7.10E-17 2
13 G152 GI-7446509 T03410 MADS box protein - maize 1 194 7.10E-17 2
13 G152 GI-1905934 MADS box protein 1 172 7.80E-17 2
13 G152 GI-7489647 T14737 MADS box protein - sorghum (fragment) 1 172 7.80E-17 2
13 G152 GI-602902 SLM2 1 190 9.40E-17 2
13 G152 GI-15667638 AF097746_1 putative MADS-box family transc... 1 205 1.10E-16 2
13 G152 GI-431226 deficiens analogue 1 192 1.20E-16 2
13 G152 GI-7446549 T07066 MADS-box protein homolog DEF4 - potato 1 192 1.20E-16 2
13 G152 GI-15216293 MADS-box protein [Rosa rugosa] 1 188 1.40E-16 2
13 G152 GI-602900 SLM1 1 198 1.50E-16 2
13 G152 GI-16024 globosa 1 195 1.50E-16 2
13 G152 GI-282981 S28062 homeotic protein globosa - garden s... 1 195 1.50E-16 2
13 G152 GI-417062 GLOB_ANTMA FLORAL HOMEOTIC PROTEIN GLOBOSA 1 195 1.50E-16 2
Table 2
13 G152 GI-2507627 putative MADS box transcription factor PrM... 192 1.50E-16 2
13 G152 GI-7446555 T10767 probable MADS box protein MADS5 - M... 192 1.50E-16 2
13 G152 GI-1370276 MADS-box protein 189 1.50E-16 2
13 G152 GI-22665 GP (green petal) 190 1.90E-16 2
13 G152 GI-322773 S31693 transcription factor gp - garden pe... 190 1.90E-16 2
13 G152 GI-729974 MAD1_PETHY FLORAL HOMEOTIC PROTEIN PMADS1 ... 190 1.90E-16 2
13 G152 GI-8163952 AF230705_1 MADS box transcription factor A... 189 1.90E-16 2
13 G152 GI-16549083 putative MADS-domain transcription factor ... 161 2.10E-16 2
13 G152 GI-2961437 MADS box protein 193 2.40E-16 2
13 G152 GI-7446535 T03902 MADS4 box protein - rice 193 2.40E-16 2
13 G152 GI-13384064 AF335243_1 MADS-box transcription factor F... 181 2.50E-16 2
13 G152 GI-511065 deficiens analogue 188 3.10E-16 2
13 G152 GI-7446533 T07410 MADS box protein homolog DEF2 - potato 188 3.10E-16 2
13 G152 GI-10880313 putative MADS-domain transcription factor ... 216 3.20E-16 1
13 G152 GI-6606072 AF058698_1 MADS15 protein 192 3.60E-16 2
13 G152 GI-309574 homologue of Arabidopsis gene AGAMOUS 189 4.30E-16 3
13 G152 GI-542191 JQ2289 floral homeotic protein ZAG1 - maize 189 4.30E-16 3
13 G152 GI-16549060 putative MADS-domain transcription factor ... 153 4.60E-16 3
Ul i 13 G152 GI-2981610 transcription factor 176 5.30E-16 3
13 G152 GI-10880315 putative MADS-domain transcription factor ... 214 5.30E-16 1
13 G152 GI-3851333 putative MADS-domain transcription factor 169 5.40E-16 2
13 G152 GI-3851331 putative MADS-domain transcription factor 158 5.60E-16 2
13 G152 GI-15667640 AF097747_1 putative MADS-box family transc... 200 6.20E-16 2
13 G152 GI-1067169 floral binding protein number 7 190 6.40E-16 2
13 G152 GI-10880311 putative MADS-domain transcription factor ... 181 1.10E-15 2
13 G152 GI-16018 DEF A protein 183 1.30E-15 2
13 G152 GI-16020 deficiens 183 1.30E-15 2
13 G152 GI-100479 S12378 defA-1 protein - garden snapdragon 183 1.30E-15 2
13 G152 GI-100483 S19232 gene deficiens protein - garden sna... 183 1.30E-15 2
13 G152 GI-118426 DEFA_ANTMA FLORAL HOMEOTIC PROTEIN DEFICIENS 183 1.30E-15 2
13 G152 GI-15077026 AF286649_1 transcription factor CMB1 [Cucu... 173 1.40E-15 3
13 G152 GI-3170498 APETALA3 homolog PcAP3 175 1.70E-15 2
13 G152 GI-8163962 AF230710_1 MADS box containing protein PI ... 172 1.80E-15 2
13 G152 GI-19386 TDR6 173 2.20E-15 2
13 G152 GI-481512 S38778 TDR6 protein - tomato (fragment) 173 2.20E-15 2
13 G152 GI-542029 S23731 TDR6 protein - tomato (fragment) 173 2.20E-15 2
Table 2
G152 GI-2055376 MADS box protein 1 170 2.70E-15 2 G152 GI-5295986 MADS box-like protein 1 180 2.80E-15 2 G152 GI-309576 homologue of Arabidopsis Agamous-like gene 1 169 3.40E-15 4 G152 GI-542192 PQ0770 floral homeotic protein ZAG2 - maiz... 1 169 3.40E-15 4 G152 GI-13442960 AF095645_1 MADS box protein nmadsl [Oryza ... 1 180 3.50E-15 2 G152 GI-3114586 MADS box protein 1 206 3.80E-15 1 G152 GI-10880319 putative MADS-domain transcription factor ... 1 153 4.90E-15 2 G152 GI-3170512 APETALA3 homolog PhAP3 1 166 5.90E-15 2 G152 GI-5019429 putative MADS domain transcription factor ... 1 204 6.20E-15 G152 GI-1362199 S55773 homeotic protein ZAG2 - maize (frag... 1 202 1.00E-14 G152 GI-3184054 MADS-box transcription factor 1 202 1.00E-14 G152 GI-6468290 putative MADS domain transcription factor ... 1 202 1.00E-14 G152 GI-7446516 T06543 MADS box protein - garden pea 1 202 1.00E-14 G152 GI-9367232 MADS box protein 1 [Hordeum vulgare] 1 184 1.20E-14 2 G152 GI-9367234 MADS-box protein 1-2 [Hordeum vulgare] 1 184 1.20E-14 2 G152 GI-6468288 putative MADS domain transcription factor ... 1 181 1.20E-14 2 G152 GI-887575 MADS box regulatory protein 1 201 1.30E-14 1 G152 GI-1046272 MADS box regulatory protein 1 201 1.30E-14 1 G152 GI-1362200 S55774 homeotic protein ZMM1 - maize (frag... 1 201 1.30E-14 1 G152 GI-17933454 MADS-box protein [Brassica napus] 1 184 1.50E-14 2 G152 GI-5051937 MADS-box protein FDRMADS2 1 156 1.90E-14 2 G152 GI-19871 NTGLOBOSA 1 199 2.10E-14 1 G152 GI-169254 transcription factor 1 199 2.10E-14 1 G152 GI-320595 JQ1689 floral binding protein 1 - garden p... 1 199 2.10E-14 1 G152 GI-417063 GLOB_TOBAC FLORAL HOMEOTIC PROTEIN GLOBOSA 1 199 2.10E-14 1 G152 GI-448288 1916408A NTGLO gene [Nicotiana tabacum] 1 199 2.10E-14 1 G152 GI-486750 S35226 homeotic protein globosa homolog - ... 1 199 2.10E-14 1 G152 GI-729464 FBP1_PETHY FLORAL HOMEOTIC PROTEIN FBP1 (F... 1 199 2.10E-14 1 G152 GI-2293892 unnamed protein product 1 199 2.10E-14 1 G152 GI-17432174 MADS-box protein [Lycopersicon esculentum] 1 199 2.10E-14 1 G152 GI-3253149 CMADS2 1 177 2.40E-14 2 G152 GI-16549062 putative MADS-domain transcription factor ... 1 165 2.50E-14 2 G152 GI-4218169 MADs-box protein, GDEF1 1 198 2.70E-14 1 G152 GI-4885034 AF134114_1 PISTILLATA protein homolog HPH 1 198 2.70E-14 1 G152 GI-4885036 AF134115_1 PISTILLATA protein homolog HPI2 1 197 3.50E-14 1 G152 GI-5295990 MADS box-like protein 1 196 4.40E-14 1
Table 2
13 G152 GI-1362203 S55938 homeotic protein ZMM4 - maize (frag... 1 195 5.60E-14 1
13 G152 GI-4218171 MADS-box protein, GDEF2 1 195 5.60E-14 1
13 G152 GI-4218173 MADS-box protein, GGL01 1 195 5.60E-14 1
13 G152 GI-10803404 MADS box protein [Gerbera hybrida] 1 194 7.20E-14 1
13 G152 GI-12002139 AF112148_1 MADS box protein 1 [Zea mays] 1 194 7.20E-14 1
13 G152 GI-3170478 PISTILLATA homolog DaPI-1 1 161 8.40E-14 2
13 G152 GI-5825623 AF180364_1 DEFICIENS homolog DEF1 1 193 9.20E-14 1
13 G152 GI-5825625 AF180365_1 DEFICIENS homolog DEF2 1 193 9.20E-14 1
13 G152 GI-8567991 AF068723_1 MADS-box protein MADS4 [Nicotia... 1 193 9.20E-14 1
13 G152 GI-3892652 putative MADS-domain transcription factor 1 159 9.80E-14 2
13 G152 GI-7677036 FDRMADS7 [Oryza sativa] 1 157 1.20E-13 2
13 G152 GI-12666533 Pistillata MADS-box protein [Malus x domes... 1 192 1.20E-13 1
13 G152 GI-12666535 Pistillata MADS-box protein [Malus x domes... 1 192 1.20E-13 1
13 G152 GI-3253153 MADS-box protein 1 177 1.30E-13 2
13 G152 GI-6092007 GpMADSI 1 168 1.30E-13 2
13 G152 GI-8163966 AF230712_1 MADS box containing protein PI ... 1 191 1.50E-13 1
13 G152 GI-3170470 PISTILLATA homolog RfPI-1 1 157 1.70E-13 2
S 13 G152 GI-695319 MADS box protein 1 189 2.50E-13 1
" 13 G152 GI-722400 MADS box protein 1 189 2.50E-13 1
13 G152 GI-1362196 S55772 homeotic protein TMZ1-I15a - maize ... 1 189 2.50E-13 1
13 G152 GI-1362198 S55768 homeotic protein ZAG1 - maize (frag... 1 189 2.50E-13 1
13 G152 GI-1561786 homeotic protein boi2AP3 1 189 2.50E-13 1
13 G152 GI-3023537 CMB2_DIACA MADS BOX PROTEIN CMB2 1 189 2.50E-13 1
13 G152 GI-6580939 AF158539_1 MADS-box transcription factor 1 189 2.50E-13 1
13 G152 GI-6580941 AF158540_1 MADS-box transcription factor 1 189 2.50E-13 1
13 G152 GI-7446538 T10715 MADS-box protein CMB2 - clove pink 1 189 2.50E-13 1
13 G152 GI-7446540 T14473 MADS box protein 2AP3 - broccoli 1 189 2.50E-13 1
13 G152 GI-1006768 ZMM2 1 163 2.70E-13 2
13 G152 GI-7446528 T04388 AGAMOUS-like MADS-box protein ZMM2 1 163 2.70E-13 2
13 G152 GI-5230654 MADS-box protein 1 161 2.80E-13 2
13 G152 GI-6635740 AF207699_1 agamous-like MADS box protein O... 1 188 3.20E-13
13 G152 GI-1561782 homeotic protein boι'1AP3 1 187 4.00E-13
13 G152 GI-6841082 AF124814_1 APETALA3 1 187 4.00E-13
13 G152 GI-793902 ZEM1 1 186 5.10E-13
13 G152 GI-793904 ZEM2 1 186 5.10E-13
13 G152 GI-793906 ZEM3 1 186 5.10E-13
Table 2
G152 GI-1076827 S54209 ZEM1 protein - maize (fragment) 186 5.10E-13
G152 GI-1362195 555770 homeotic protein TMZ1-I20a - maize ... 186 5.10E-13
G152 GI-1362197 555771 homeotic protein TMZ1-I5a - maize (... 186 5.10E-13
G152 GI-1362208 556754 ZEM1 protein - maize (fragment) 186 5.10E-13
G152 GI-1362209 556755 ZEM2 protein - maize (fragment) 186 5.10E-13
G152 GI-939783 MADS box protein 185 6.60E-13
G152 GI-7489788 T03409 MADS box protein - maize (fragment) 185 6.60E-13
G152 GI-16549064 putative MADS-domain transcription factor ... 156 6.70E-13
G152 GI-3253147 CMADS1 192 7.10E-13
G152 GI-9857312 MADS-box protein [Rosa rugosa] 184 8.40E-13
G152 GI-17933450 MADS-box protein [Brassica napus] 184 8.40E-13
G152 GI-3170486 PISTILLATA homolog PmPI-1 150 9.40E-13
G152 GI-1944532 homeotic protein 183 1.10E-12
G152 GI-3253151 MADS-box protein 183 1.10E-12
G152 GI-5295988 MADS box-like protein 183 1.10E-12
G152 GI-3170462 APETALA3 homolog RfAP3-1 150 1.20E-12
G152 GI-1621333 homeotic protein bobap3 182 1.40E-12
G152 GI-2507631 putative MADS box transcription factor PrM... 182 1.40E-12
G152 GI-7446541 T14474 MADS box protein ap3 - broccoli 182 1.40E-12
G152 GI-7484486 T10777 probable MADS box protein MADS7 - M... 182 1.40E-12
G152 GI-17933452 MADS-box protein [Brassica napus] 182 1.40E-12
G152 GI-6465899 Apetala 1 protein 168 1.40E-12
G152 GI-6465897 Apetala 1 protein 152 1.70E-12
G152 GI-17933456 MADS-box protein [Brassica napus] 180 2.20E-12
G152 GI-17933458 MADS-box protein [Brassica napus] 177 4.70E-12
G152 GI-15022157 MADS box protein-like protein NGL9 [Medica... 176 6.00E-12
G152 GI-3170476 PISTILLATA homolog SvPI-1 147 6.40E-12
G152 GI-6465895 Apetala 1 protein 155 7.70E-12
G152 GI-887577 MADS box regulatory protein 172 1.60E-11
G152 GI-1046274 MADS box regulatory protein 172 1.60E-11
G152 GI-3170468 PISTILLATA homolog DePI-1 155 1.90E-11
G152 GI-14573447 AF381766_1 apetala3-like protein [Mimulus ... 171 2.10E-11
G152 GI-14573449 AF381767_1 apetala3-like protein [Mimulus ... 169 3.30E-11
G152 GI-16549066 putative MADS-domain transcription factor ... 169 3.30E-11
G152 GI-602904 SLM3 168 4.30E-11
G152 GI-1362204 S55776 homeotic protein ZMM6 - maize (frag... 167 5.50E-11
Table 2
13 G152 GI-2459835 MADS-domain protein 166 7.00E-11 1
13 G152 GI-1362205 S55775 homeotic protein ZMM7 - maize (frag... 165 8.90E-11 1
13 G152 GI-6069652 similar to MADS-box protein AGL14 (U20184) 178 1.70E-10 1
13 G152 GI-5712748 AF159710_1 MADS-box transcription factor 162 1.90E-10 1
15 G153 AMDEFH125 Y10750 A.majus mRNA for DEFH125 protein. 8/... 267 1.20E-36 3
15 G153 AW706936 AW706936 sk08d08.y1 Gm-c1023 Glycine max cD... 2 283 1.50E-35 3
15 G153 AP004526 AP004526 Lotus japonicus genomic DNA, chrom... -2 273 1.20E-30 4
15 G153 AW218280 AW218280 EST303461 tomato radicle, 5 d post... 3 280 6.60E-30 2
15 G153 BG651806 BG651806 sad61c04.y1 Gm-c1051 Glycine max c... 3 280 4.40E-28 2
15 * G153 BM405213 BM405213 EST579540 potato roots Solanum tub... 2 276 9.30E-28 2
15 G153 AI974336 AI974336 T110185e KVO Medicago truncatula c... 2 272 1.50E-26 2
15 G153 AX085151 AX085151 Sequence 1 from Patent WO0112798. ... 1 269 9.70E-25 2
15 G153 AX085348 AX085348 Sequence 1 from Patent WO0112799. ... 1 269 9.70E-25 2
15 G153 AF112149 AF112149 Zea mays MADS box protein 2 (mads2... 1 269 9.70E-25 2
15 G153 MSU91964 U91964 Medicago sativa MADS-box protein mRN... 1 243 1. OOE-24 2
15 G153 AL389596 AL389596 MtBC56A01 F1 MtBC Medicago truncatu... 2 239 1.40E-23 2
15 G153 AX085162 AX085162 Sequence 12 from Patent WO0112798.... 1 262 5.20E-22 2
15 G153 AX085359 AX085359 Sequence 12 from Patent WO0112799.... 1 262 5.20E-22 2
Ul -4 15 G153 AP003868 AP003868 Oryza sativa chromosome 8 clone OJ... 3 252 7.20E-22 3
15 G153 BM268642 BM268642 MEST398-C12.univ ISUM5-RN Zea mays... -3 280 1.30E-20 1
15 G153 BM334225 BM334225 MEST134-C05.T3 ISUM5-RN Zea mays c... -1 280 1.50E-20 1
15 G153 BG592879 BG592879 EST491557 cSTS Solanum tuberosum c... 3 280 1.50E-20 1
15 G153 BE341755 BE341755 EST394587 potato stolon, Cornell U... 3 280 1.90E-20 1
15 G153 BE034403 BE034403 MH04D03 MH Mesembryanthemum crysta... 3 276 2.70E-20 1
15 G153 AQ917367 AQ917367 T233384b Medicago truncatula BAC I... 2 274 6.70E-20 1
15 G153 OSJN00003 AL606460 Oryza sativa chromosome 4 clone OS... 1 273 8.20E-20 2
15 G153 AP004766 AP004766 Oryza sativa (japonica cultivar-gr... 1 260 1.30E-19 2
15 G153 BE596704 BE596704 PI1_58_F04.b1_A002 Pathogen induce... 1 270 1.60E-19 1
15 G153 BM323459 BM323459 PIC1_19_E07.b1_A002 Pathogen-infec... 3 270 1.90E-19 1
15 G153 BE319808 BE319808 NF020A03RT1F1017 Developing root M... 3 268 3.20E-19 1
15 G153 BE354988 BE354988 DG1_10_H09.b1_A002 Dark Grown 1 (D... 2 267 4.30E-19 1
15 G153 RICOSMA L37526 Oryza sativa MADS-box protein (MADS2... 2 259 1.30E-18 1
15 G153 ZMA292959 AJ292959 Zea mays mRNA for putative MADS-do... 1 256 2.90E-18 1
15 G153 MDU78949 U78949 Malus domestica MADS-box protein 3 m... 1 224 3.60E-18 2
15 G153 AW330598 AW330598 707037C10.x1 707 - Mixed adult tis... 2 256 4.90E-18 1
15 G153 BE445262 BE445262 WHE1133_A05_A09ZS Wheat etiolated ... 2 256 5.00E-18 1
Table 2
G153 BE186351 BE186351 945040C10.X1 945 - Mixed adult tis... 1 256 5.70E-18 1
G153 MDAJ761 AJ000761 Malus domestica mRNA for MADS-box ... 1 224 6.40E-18 2
G153 AP004616 AP004616 Oryza sativa chromosome 6 clone PO... -2 247 1.20E-17 2
G153 AF305696 AF305696 Eucalyptus globulus MADS-box prote... 1 215 4.70E-17 2
G153 AF306349 AF306349 Eucalyptus globulus MADS box prote... 1 215 6.80E-17 2
G153 BF484557 BF484557 WHE2317_A03_A05ZS Wheat pre-anthes... 1 243 1.10E-16 1
G153 BE493790 BE493790 WHE1275_E07_I13ZS Secale cereale a... 2 243 1.20E-16 1
G153 BE495206 BE495206 WHE1269_F04_L07ZS Secale cereale a... 1 243 1.40E-16 1
G153 BJ195968 BJ195968 BJ195968 normalized full length cD... 2 242 1.80E-16 1
G153 BG445265 BG445265 GA Ea0027H05f Gossypium arboreum ... 2 239 2.00E-16 1
G153 BG444491 BG444491 GA Ea0024l13f Gossypium arboreum ... 3 239 2.00E-16 1
G153 BG445047 BG445047 GA Ea0026l23f Gossypium arboreum ... 3 239 2.20E-16 1
G153 BG241308 BG241308 OV1_40_G05.b1_A002 Ovary 1 (OV1) S... 2 241 2.40E-16 1
G153 BG240086 BG240086 OV1_17_G05.b1_A002 Ovary 1 (OV1) S... 2 241 2.40E-16 1
G153 BG441266 BG441266 GA Ea0012l13f Gossypium arboreum ... 2 239 2.60E-16 1
G153 MDAJ762 AJ000762 Malus domestica mRNA for MADS-box ... 3 238 2.80E-16 1
G153 AF130118 AF130118 Capsicum annuum MADS box protein (... 1 237 3.20E-16 1
G153 AB050657 AB050657 Magnolia praecocissima mRNA for pu... 1 237 3.30E-16 1
G153 BG444639 BG444639 GA Ea0025A08f Gossypium arboreum ... 2 237 3.40E-16 1
G153 BG441292 BG441292 GA_Ea0012J14f Gossypium arboreum ... 2 237 3.60E-16 1
G153 BG412085 BG412085 OV2_37_G09.b1_A002 Ovary 2 (OV2) S... 3 239 3.80E-16 1
G153 BG445079 BG445079 GA Ea0026K23f Gossypium arboreum ... 3 237 4.00E-16 1
G153 AI731368 AI731368 BNLGHi9370 Six-day Cotton fiber Go... 2 237 4.00E-16 1
G153 BI311053 BI311053 EST5312803 GESD Medicago truncatul... 1 237 4.10E-16 1
G153 AI731375 AI731375 BNLGHi9382 Six-day Cotton fiber Go... 2 237 4.10E-16 1
G153 BM359874 BM359874 GA Ea0024l13r Gossypium arboreum ... 2 239 4.10E-16 1
G153 ZMA292961 AJ292961 Zea mays mRNA for putative MADS-do... 3 249 4.10E-16 1
G153 BE659915 BE659915 7-H4 GmaxSC Glycine max cDNA, mRNA... 3 237 4.40E-16 1
G153 ZMA292960 AJ292960 Zea mays mRNA for putative MADS-do... 1 245 4.60E-16 1
G153 BG440218 BG440218 GA_Ea0006G19f Gossypium arboreum ... 2 237 4.60E-16 1
G153 AW184799 AW184799 se82f12.y1 Gm-c1023 Glycine max cD... 2 237 4.70E-16 1
G153 AI725968 AI725968 BNLGHM3738 Six-day Cotton fiber G... 2 237 4.80E-16 1
G153 RICOSMAA L37527 Oryza sativa MADS box protein (MADS4... 3 238 4.90E-16 1
G153 BF276744 BF276744 GA Eb0030l01f Gossypium arboreum ... 2 238 4.90E-16 1
G153 BE659913 BE659913 3-D9 GmaxSC Glycine max cDNA, mRNA... 1 237 5.00E-16 1
G153 AW705451 AW705451 sk49c05.y1 Gm-c1019 Glycine max cD... 2 237 5.10E-16 1
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Figure imgf000160_0001
rs. ιs. rs. rs. rs. rs. |s. rs. cD co cn ιs. m rr rr rr rr rr cD rr m m rr m rr m co m m m rr m m m co
CO CO CO C») C CO C CO CO C CO C CO Cr) Co CO CO CO CO CO C CO CO CO C CO CO CO CO CO C C CO C C CO CM CM CM CM CM CM C Og CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM OJ OJ OJ CM CM CM CM CM OJ OJ OJ CM
Figure imgf000160_0002
C2 CO CO C CO C0 C CO C CO CO C C C0 C0 CO C C C C C CO CO C C C0 C CO CO CO CO CO C CO CO CO
Lo m m m m m m m m m m m m m m m m m m m m m m m in m m m m m ϊn m LO LO δδoδδδδδδδδδδδδδδδδδδδδδδδδδδδ m m m m m m m m m m m m m m m m m m m m m m m m m m m m m Table 2
15 G153 BG412023 BG412023 OV2_37_A12.b1_A002 Ovary 2 (0V2) S... 3 235 9.90E-16 1
15 G153 BF657695 BF657695 OV2_20_F03.b1_A002 Ovary 2 (OV2) S... 3 235 9.90E-16 1
15 G153 AV933011 AV933011 AV933011 K. Sato unpublished cDNA ... 1 234 1.00E-15 1
15 G153 A 442341 AW442341 EST311737 tomato fruit red ripe, T... 3 234 1.00E-15 1
15 G153 BE455888 BE455888 HVSMEg0017B20f Hordeum vulgare pre... 1 233 1.00E-15 1
15 G153 BI958544 BI958544 HVSMEn0015l07f Hordeum vulgare rac... 3 234 1.00E-15 1
15 G153 BE500707 BE500707 WHE0991-0994J21J21ZS Wheat pre-a... 3 235 1.00E-15 1
15 G153 BE499104 BE499104 WHE0960_C01_E02ZS Wheat pre-anthes... 3 234 1.00E-15 1
15 G153 AF072534 AF072534 Capsicum annuum pepper MADS-box pr... 1 233 1.00E-15 1
15 G153 BG054384 BG054384 OV2_3_G05.b1_A002 Ovary 2 (OV2) So... 3 237 1.10E-15 1
15 G153 BF657168 BF657168 OV2_21_E10.b1_A002 Ovary 2 (OV2) S... 3 235 1.10E-15 1
15 G153 BE432640 BE432640 EST399169 tomato breaker fruit, TI... 1 234 1.10E-15 1
15 G153 BG442607 BG442607 GA Ea0017K04f Gossypium arboreum ... 3 234 1.10E-15 1
15 G153 AV934186 AV934186 AV934186 K. Sato unpublished cDNA ... 2 234 1.10E-15 1
15 G153 AV932867 AV932867 AV932867 K. Sato unpublished cDNA ... 2 234 1.10E-15 1
15 G153 BF429319 BF429319 WHE1804_B03_D06ZS Secale cereale a... 3 234 1.10E-15 1
15 G153 BE430838 BE430838 SUN006.D03F991220 ITEC SUN Wheat c... 1 233 1.10E-15 1 15 G153 AI728519 AI728519 BNLGHi10938 Six-day Cotton fiber G... 2 234 1.10E-15 1 15 G153 BE454527 BE454527 HVSMEh0094C05f Hordeum vulgare 5-4... 1 232 1.10E-15 1
15 G153 AV938919 AV938919 AV938919 K. Sato unpublished cDNA ... 3 234 1.10E-15 1
15 G153 BG240312 BG240312 OV1_20_E03.b1_A002 Ovary 1 (OV1 ) S... 2 235 1.20E-15 1
15 G153 BF657564 BF657564 OV2_23_G05.b1_A002 Ovary 2 (OV2) S... 1 235 1.20E-15 1
15 G153 BF728292 BF728292 1000060C02.X1 1000 - Unigene I fro... 1 234 1.20E-15 1
15 G153 BI272502 BI272502 NF023F04F 1 F1042 Developing flower... 1 235 1.20E-15 1 5 G153 C95289 C95289 C95289 Citrus unshiu Miyagawa-wase m... 1 235 1.20E-15 1 5 G153 BG415259 BG415259 HVSMEk0005M04f Hordeum vulgare tes... 2 232 1.20E-15 1 5 G153 BF622888 BF622888 HVSMEa0009O05f Hordeum vulgare see... 2 233 1.20E-15 1 5 G153 BM374839 BM374839 EBma05_SQ003_C14_R IGF Barley EBma... 1 234 1.20E-15 1 5 G153 AW931475 AW931475 EST357318 tomato fruit mature gree... 1 234 1.20E-15 1
15 G153 AF254557 AF254557 Oryza sativa MADS (MADS) gene, com... 3 254 1.20E-15 1
15 G153 BE454695 BE454695 HVSMEh0095C18f Hordeum vulgare 5-4... 3 232 1.20E-15 1 5 G153 BI928852 BI928852 EST548741 tomato flower, 3 - 8 mm ... 1 234 1.20E-15 1 5 G153 BF484655 BF484655 WHE2318_B04_C08ZS Wheat pre-anthes... 2 234 1.30E-15 1
15 G153 AF198175 AF198175 Dendrobium grex Madame Thong-IN MA... 2 232 1.30E-15 1
15 G153 AW220779 AW220779 EST297248 tomato fruit mature gree... 1 234 1.30E-15 1
15 G153 BG414586 BG414586 HVSMEk0002P16f Hordeum vulgare tes... 3 232 1.30E-15 1
m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m
LU IJJ IJLI LI LJJ LJJ LJJ LJJ LU LJJ IJ^ o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o co co co rr m m m m m m m m m m m m m co co co co cD co co co r-. r-. oo oo oo cn o) 0) 0)
CO rT CM rr cO CO rr CM rr θ) CO r- CM CM CM OI CM C CO CM CO rr rr rr cθ CM rr cθ CM CM CO 'r- CO O CM O co co co c co co co co co rr co co co co co co co co co co co m m m co co co co co co co co co co co co
CM CM CM OJ CM CM CM CM OJ OJ OJ CM CM CM CM CM CM CM OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ CM CM CM CM CM CM
CO i- CM CO O OO CO CO CM CM i- '" T '7 (ll '- W CO CM C r- CO ι- τ- Cθ n r-
Figure imgf000162_0001
O) S 1^ 3 CM CO « CD m ° t f <J> CI! r- U) J J lO r- n N CD .n C '^ ΣS S cO CΛ O
K ^ S S S S w to - S m m β j co i- i n Λ s o s o to oi w θ "2 co ,_ ? rr oo rf in f i ^ 5Λ _ rr c S 5P rr ca ιs. o o rr r. -# rr c rf o) 'i- co cM ^ ^ co oj f . o co
W n lS S 2 ro N n ffl o fe S ϊ c0 0) ^ 2 S τ- o [ W ™ 5 N ™ ^ S » « ^ N ^ O c p σ> c co ^ κ S Lo c 52 P θ θ θ θ ^ Λ o ^ Λ o o o) co cn c ^ S^ c - r-. c c ιn
2 [_ Ul lι lL lU -; -! S LL t O j J J J J llJ lU j l- ϋ Q. lL LL Ll. S ιL -! Lj S ϋ ϊ ^ 00 _I CQ CQ OQ αj CD CO O < < CQ < < < < < CQ CQ < CO < < < CQ Qj O OO CD < CO CQ CQ < CQ < co co c co co co c co co co co co n co co co co co co co co co co co co w co co co co co eo co co n m m m m m m m m m m m m m m m m m ^ ^ δδδδδδδδδ δδδδδδδδδδδδδδδδδδδδδδδδδδ mmmm mmmmmmmm mmmmmmmm mmmmm mmmmmm
m m m m m m m m m m mm m m m m m m m m m m m m m m m m m m m m
LXJ I J LJ UJ U-I LU LJJ LJJ LJJ LIJ LJ^ O O O O O O O O O O O O O P P O O O O O O O O O O O O O O O O O O O O O cΛ θ o o σ o o ι- -ι- t- - ^ τ- c C c c co o co c co co o co rr rr rr rr rr ). r ^ L m ■Γ-' C CJ CM' CM' CM' CM CM CM CM' CM' CM' OJ C CM' CM OJ' CM CM' OJ OJ OJ CM' CM' CM' C CM' CM
CN
Figure imgf000163_0001
S σ> o S N δ ffl ιn β ? 2 N ffl o n 5 n N « S o SJ ; * u)
§ 2 2 § LU 3 § 5 -2 -* ≤ O LU § 2 LL Σ § I: O § ° £ 5 -I: 2 LU
Figure imgf000163_0002
C0 < 0Q C0 < CQ < < < < < < CQ 0Q < < CQ < < C0 CQ CQ < < C0 < < C0 00 m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m ooδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m Table 2
G153 AI488813 AI488813 EST247152 tomato ovary, TAMU Lycop... 1 231 2.50E-15
G153 AW933623 AW933623 EST359466 tomato fruit mature gree... 1 231 2.50E-15
G153 AI898519 AI898519 EST267962 tomato ovary, TAMU Lycop... 3 231 2.50E-15
G153 AP003908 AP003908 Oryza sativa chromosome 8 clone OJ... -1 252 2.60E-15
G153 AI895411 AI895411 EST264854 tomato callus, TAMU Lyco... 1 231 2.70E-15
G153 ZMA271208 AJ271208 Zea mays mRNA for putative MADS-do... 3 240 2.70E-15
G153 AY036062 AY036062 Brassica napus SHATTERPROOF1 (BnSH. 1 229 2.80E-15
G153 A36587 A36587 Sequence 1 from Patent WO9400582. 3/... 2 229 2.80E-15
G153 PETTRNSFA M91190 Petunia transcription factor (fbp 1 ) ... 2 229 2.80E-15
G153 GGN132208 AJ132208 Gnetum gnemon mRNA for putative MA... 3 231 2.80E-15
G153 NTGLOBOSA X67959 N.tabacum GLO mRNA. 6/1993 1 229 2.80E-15
G153 BE323629 BE323629 NF006D03PL1 F1026 Phosphate starved... 2 232 2.90E-15
G153 BE433290 BE433290 EST399819 tomato breaker fruit, TI... 3 231 2.90E-15
G153 AF023615 AF023615 Pinus radiata MADS box protein mRN... 2 228 3.00E-15
G153 AF345246 AF345246 Ipomoea batatas MADS box transcrip... 1 228 3.10E-15
G153 BF728277 BF728277 1000060A05.X1 1000 - Unigene I fro... 1 230 3.10E-15
G153 BG599624 BG599624 EST504519 cSTS Solanum tuberosum c... 1 229 3.10E-15
G153 AF335237 AF335237 Petunia x hybrida MADS-box transcr... 1 228 3.10E-15
G153 AI898432 AI898432 EST267875 tomato ovary, TAMU Lycop... 1 229 3.10E-15
G153 AI487420 AI487420 EST245742 tomato ovary, TAMU Lycop... 1 229 3.30E-15
G153 BM405355 BM405355 EST579682 potato roots Solanum tub... 1 229 3.50E-15
G153 BF112397 BF112397 EST439987 tomato breaker fruit Lye... 3 232 3.60E-15
G153 BG417142 BG417142 HVSMEk0016H10f Hordeum vulgare tes... 1 230 3.60E-15
G153 AF112150 AF112150 Zea mays MADS box protein 3 (mads3... 1 244 3.80E-15
G153 LETDR3 X60756 L.esculentum TDR3 mRNA. 3/1992 3 229 3.80E-15
G153 BH744278 BH744278 gt35a01.b1 BoBudsOI Brassica olera... 1 229 3.90E-15
G153 AF101420 AF101420 Cichorium intybus MADS box protein... 1 230 4.00E-15
G153 AW928593 AW928593 EST337381 tomato flower buds 8 mm ... 2 232 4.10E-15
G153 BI948755 BI948755 HVSMEI0010L12f Hordeum vulgare spi... 3 227 4.20E-15
G153 AC092556 AC092556 Oryza sativa chromosome 3 clone OS... 2 231 4.30E-15 2
G153 AF377947 AF377947 Oryza sativa chromosome 3 clone OS... -3 231 4.40E-15 2
G153 BF276751 BF276751 GA Eb0030l08f Gossypium arboreum ... 2 229 4.50E-15
G153 BG417111 BG417111 HVSMEk0016F04f Hordeum vulgare tes... 3 229 4.60E-15
G153 BG274189 BG274189 WHE2230_F01_K02ZS Aegilops speltoi... 2 229 4.70E-15
G153 BE193581 BE193581 HVSMEh0081 M10f Hordeum vulgare 5-4... 2 227 4.80E-15
G153 AW934066 AW934066 EST359909 tomato fruit mature gree... 3 229 4.80E-15
mmmmmmm mmmmm mmmmm
LU LU LU LU LU LU LU O O O O O O O O O O O O O O O O O O
CO O O r r O i h β OI O O IO N S O CO rt m' m' m m m' iri
Figure imgf000165_0001
co cjd cβ cc cn m co c)θ cΛ CΛ m - rs- co cn ^ 'i- m co m co rs. oo co m oo rs. rr 'i-- o rr m cΛ rr cM rr co rr J CM rr cM CM C CM CM CO CM CΛJ CM rr rr OJ OJ OJ OJ OJ CO Cλl CΛI C CM CO CO rr rr CM CM CM CO CO CM CM CM CM CM CM C CM CM CM CM CM OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ CM CM I CM CM CM CM CM CM CM OJ OJ CM CM CM
CM '- i- i- C i- T- I t CO i- N N CM '- '- '- N CV C CO T- N '- W '- N C T- r- i- T- CM - CO
CN
Figure imgf000165_0002
c2 co co co eo co co co co co c co co co co co c co co co co co co co co co co co co co co co co co co co m m m m m m m m m m m m m m m m m m m m m m m m m m m Ln Ln Lo m m m m m δδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδδ δδδ mmmmmmmmmmmmmmmm mmmmmmmmmmmmmmmm mm Table 2
15 G153 AF095645 AF095645 Oryza sativa MADS box protein nmad... 2 224 9.80E-15 1
15 G153 AU095354 AU095354 AU095354 Rice cDNA from immature I... 2 226 9.90E-15 1
15 G153 AX006357 AX006357 Sequence 6 from Patent WO0004176. ... 2 239 9.90E-15 1
15 G153 AF035438 AF035438 Cucumis sativus MADS box protein C... 3 239 9.90E-15 1
15 G153 BI434165 BI434165 EST536926 P. infestans-challenged ... 1 224 1.00E-14 1
15 G153 AF022379 AF022379 Cucumis sativus agamous-like putat... 1 239 1.00E-14 1
15 G153 BG591718 BG591718 EST499560 P. infestans-challenged ... 1 224 1.00E-14 1
15 G153 AF345911 AF345911 Oryza sativa MADS-box protein FDRM... 2 241 1.00E-14 1
15 G153 MZEMADSD L46400 Zea mays MADS-box protein (ZAP1 ) mRN... 2 241 1.00E-14 1
15 G153 CRCRM3 Y08239 C.richardii mRNA for CRM3 protein. 7... 2 223 1.10E-14 1
15 G153 BG445408 BG445408 GA Ea0028G20f Gossypium arboreum ... 3 223 1.10E-14 1
15 G153 AI489508 AI489508 EST247847 tomato ovary, TAMU Lycop... 3 225 1.10E-14 1
15 G153 AB050649 AB050649 Magnolia praecocissima mRNA for pu... 223 1.10E-14 1
15 G153 BG631100 BG631100 cC-esflcLEL6H14a1 Tomato flower Ii... 226 1.10E-14 1
15 G153 BE330897 BE330897 so89f06.y1 Gm-c1041 Glycine max cD... 224 1.20E-14 1
15 G153 AF103903 AF103903 Liquidambar styraciflua AGAMOUS ho... 239 1.20E-14 1
15 G153 GGN132216 AJ132216 Gnetum gnemon mRNA for putative MA... 223 1.20E-14 1 - 15 G153 BG889771 BG889771 EST515622 cSTD Solanum tuberosum c... 3 224 1.20E-14 1 a is G153 BH712678 BH712678 BOHV069TF BO_2_3_KB Brassica olera... -2 223 1.20E-14 1
15 G153 STU23758 U23758 Solanum tuberosum transcription fact... 3 236 1.20E-14 1
15 G153 BG837838 BG837838 Zm10_02b03_A Zm10_AAFC_ECORC_Fusar... -1 224 1.20E-14 1
15 G153 BG837866 BG837866 Zm10_03d05_A Zm10_AAFC_ECORC_Fusar... -1 224 1.20E-14 1
15 G153 AF335236 AF335236 Petunia x hybrida MADS-box transcr... 1 225 1.30E-14 1
15 G153 BE346434 BE346434 sp25e06.y1 Gm-c1042 Glycine max cD... 1 224 1.30E-14 1
15 G153 AI899235 AI899235 EST268678 tomato ovary, TAMU Lycop... 1 225 1.30E-14 1
15 G153 AW929235 AW929235 EST338023 tomato flower buds 8 mm ... 3 224 1.30E-14 1
15 G153 BE315880 BE315880 NF027E08LF1F1055 Developing leaf M... 1 226 1.30E-14 1
15 G153 AF265562 AF265562 Vitis vinifera MAD-box transcripio... 2 238 1.30E-14 1
15 G153 STU23757 U23757 Solanum tuberosum transcription fact... 1 236 1.30E-14 1
15 G153 BI893993 BI893993 sai58e09.y1 Gm-c1068 Glycine max c... 3 224 1.30E-14 1
15 G153 AW277299 AW277299 sf80e05.y1 Gm-c1019 Glycine max cD... 3 224 1.40E-14 1
15 G153 AF035439 AF035439 Cucumis sativus MADS box protein C... 3 235 1.40E-14 1
15 G153 BE456120 BE456120 HVSMEg0019N18f Hordeum vulgare pre... 3 222 1.40E-14 1
15 G153 BG440326 BG440326 GA Ea0007H23f Gossypium arboreum ... 3 223 1.40E-14 1
15 G153 BM536192 BM536192 EST589214 tomato breaker fruit Lye... 2 228 1.40E-14 1
15 G153 AV937388 AV937388 AV937388 K. Sato unpublished cDNA ... 3 223 1.40E-14 1
Table 2
G153 SCAF002666 AF002666 Solanum commersonii MADS box trans... 2 236 1.40E-14 1
G153 AB041020 AB041020 Oryza sativa RAP1 B mRNA for AP1-N... 3 237 1.40E-14 1
G153 BE249689 BE249689 NF020D04LF1 F1041 Developing leaf M... 3 227 1.40E-14 1
G153 AF335244 AF335244 Petunia x hybrida MADS-box transcr... 1 222 1.40E-14 1
G153 AF265554 AF265554 Cucumis sativus transcription fact... 3 236 1.50E-14 1
G153 SAU25695 U25695 Sinapis alba transcription factor Sa... 2 228 1.50E-14 1
G153 AW186417 AW186417 se67e07.y1 Gm-c1019 Glycine max cD... 3 223 1.50E-14 1
G153 AI484747 AI484747 EST243008 tomato ovary, TAMU Lycop... 3 223 1.50E-14 1
G153 AI770967 AI770967 606063H06.x1 606 - Ear tissue cDNA... 1 224 1.50E-14 1
G153 BM500137 BM500137 PAC000000000248 Pioneer AF-1 array... 1 223 1.60E-14 1
G153 AF035378 AF035378 Lolium temulentum MADS-box protein... 2 238 1.60E-14 1
G153 BCA251300 AJ251300 Brassica rapa subsp. pekinensis mR... 1 222 1.60E-14 1
G153 BE339799 BE339799 EST343859 potato stolon, Cornell U... 1 224 1.60E-14 1
G153 BNADBDA L36927 Brassica oleracea (subspecies botryt... 1 222 1.60E-14 1
G153 BNADBD L36926 Brassica oleracea (clone pBS85) BoCA... 1 222 1.60E-14 1
G153 AW506862 AW506862 660060F01.y1 660 - Mixed stages of... 3 223 1.60E-14 1
G153 BG138098 BG138098 EST478540 wild tomato pollen Lycop... 2 223 1.60E-14 1
G153 AF150932 AF150932 Physcomitrella patens MADS-domain ... 1 243 1.60E-14 1
G153 AW309115 AW309115 sf93e09.y1 Gm-c1019 Glycine max cD... 3 223 1.60E-14 1
G153 BG139571 BG139571 EST480109 wild tomato pollen Lycop... 2 223 1.70E-14 1
G153 AW745210 AW745210 LG1_387_D10.b1_A002 Light Grown 1 ... 3 223 1.70E-14 1
G153 BG357712 BG357712 OV2_29_D08.b1_A002 Ovary 2 (OV2) S... 3 223 1.70E-14 1
G153 BF050801 BF050801 EST435959 tomato developing/immatu... 2 223 1.70E-14 1
G153 AF150934 AF150934 Physcomitrella patens MADS-domain ... 1 243 1.80E-14 1
G153 BG357882 BG357882 OV2_31_D08.b1_A002 Ovary 2 (OV2) S... 3 223 1.80E-14 1
G153 AH64140 AI164140 A055P40U Hybrid aspen plasmid libr... 2 224 1.80E-14 1
G153 BG649748 BG649748 RHIZ2_83_B06.b1_A003 Rhizome2 (RHI... 2 223 1.80E-14 1
G153 AB025645 AB025645 Rosa rugosa MASAKO C2 mRNA for MAD.. 1 235 1.80E-14 1
G153 RICOSMAB3A L37528 Oryza sativa MADS-box protein (MADS3... 1 239 1.80E-14 1
G153 AF043255 AF043255 Cucumis sativus MADS box protein 2... 3 221 1.80E-14 1
G153 BM500293 BM500293 PAC000000001427 Pioneer AF-1 array... 1 223 1.80E-14 1
G153 AW222490 AW222490 EST299301 tomato fruit red ripe, T... 2 223 1.90E-14 1
G153 TOBNAG1A L23925 Nicotiana tabacum NAG1 mRNA, complet... 2 236 1.90E-14 1
G153 BI957577 BI957577 HVSMEn0010D09f Hordeum vulgare rac... 2 222 1.90E-14 1
G153 AB022666 AB022666 Gnetum parvifolium GpMADS4 mRNA, c... 1 238 1.90E-14 1
G153 BE659970 BE659970 314 GmaxSC Glycine max cDNA, mRNA ... 2 223 2.00E-14 1
Ul LU LU LJJ LU Lij Ul LU LU LU LU LU Uj LLI LU LU lϊl lij ώ ώ O O O O O O O O O O O O O P P P O O O O O O P P P P P P O O O O O O O O o -r- ^ oi cM CM CM CM CM co co co rr rr rr m m m in in cq co co m CM' CM CM' CM OU OJ CM CM CM CM' CM' CM' CM' CM' CM CM CM CM' CM' CM' CM' C CM' W co co rf rr rr co o cM i- CM T- T- m m T- rr cM -^ rs. rs. rs. oj i- i- o j T- cn co co cM i-
CM CM OO CM CO CM CO CM CM CO CM OJ OJ CO CO CM CM CM CM CO CO CO CM OJ OJ CM CM CM CM CO CM OO CO CM OI CM CM CM OI C C CM CM CM CM CM CM CM CM CM I OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ CM CM OI CM CM CM
Figure imgf000168_0001
5S [o fc 5? cΛ Ln -^ g ^ι cM o m c cM ,. cΛ _ oo g) m rf co o 5S n £- r-. SJ co rr m
^ n S S lfi fe ^ ^ S ^ ^ m S ^ ^ ϊ ^ o S o in t f in C C O ^ g ^ r J , ^ ,, ιj ≤ LL iL θ LL LL σ L θ ^ o x χ i -= m -2 L Q LU § u !lj X LL r-- LU LU
CQ < < CQ < m < < < < m CO CQ <: < fcD < fcO < < CQ < CQ m cθ _l < CQ CQ < CO CL < O CQ CO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CmO CO CmO CmO CmO CmO CmO CmO CmO CmO CmO CO CmO CmO CmO CmO CmO CmO CmO oδoδδδδδδδδδδδδδδδδδδδδδδδ δδδδδδδδδ mmmmm mmmmmmm mmmmm mmmm mmmmmmmmmmm
rr rf rf rr rr rr rr rr rr rr rr rr rf rr rr rr rr rr rr rr rr rr rr rr rf rf rr rr rr rr rr rr rr rr rr rr
LU LLI LU lϊj LU LU l±l LU lJJ LU LU LU LU LU LU lJ LU UJ LU ώ ώ
CM τ- Cy| C ^ - Cθ - τ- CO CM ι- ι- ι- C ι- >- ι- ( W τ- C n c\I C τ- τ- CO C\I C CM I '- CO CM W
CN
Figure imgf000169_0001
co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co m m m m m m m m m m m m m m m m m m m m m m m m m m m in m rn m in m δoδoδδδδδδδδδδδδδδδδ δδδδδδδδδδδδδδδ mmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmm Table 2
G153 TOMTAG1A L26295 Lycopersicon esculentum TAG1 mRNA, c... 3 232 4.40E-14 1
G153 GHY9722 AJ009722 Gerbera hybrida mRNA for MADS-box ... 1 234 4.40E-14 1
G153 BM137344 BM137344 WHE2632_F08_L16ZS Wheat Fusarium g. 1 219 4.40E-14 1
G153 BM732175 BM732175 sal74a08.y1 Gm-c1061 Glycine max c... 3 219 4.50E-14 1
G153 AL374255 AL374255 MtBB05E10F1 MtBB Medicago truncatu... 2 220 4.50E-14 1
G153 BI543775 BI543775 FS6_F03 Sugar Beet inflorescence c... 2 218 4.50E-14 1
G153 BE918053 BE918053 OV1_1_C11.b1_A002 Ovary 1 (OV1 ) So... 1 219 4.50E-14 1
G153 BF202778 BF202778 WHE1784_E01_I02ZS Wheat pre-anthes... 2 220 4.50E-14 1
G153 BE474886 BE474886 sp69f12.y1 Gm-c1044 Glycine ma cD... 3 220 4.60E-14 1
G153 AL508733 AL508733 AL508733 Hordeum vulgare Barke dev... 2 218 4.60E-14 1
G153 BE918762 BE918762 OV1_4_F11.b1_A002 Ovary 1 (OV1 ) So... 1 219 4.60E-14 1
G153 BE431095 BE431095 SUN011.C09F991223 ITEC SUN Wheat c. 1 218 4.60E-14 1
G153 SAU25696 U25696 Sinapis alba transcription factor Sa... 1 217 4.70E-14 1
G153 PETFHPP L33973 Petunia inflata (clone L3C1) agamous... 1 234 4.70E-14 1
G153 BI497772 BI497772 sag27b04.y1 Gm-c1081 Glycine max c... 3 219 4.70E-14 1
G153 AF134115 AF134115 Hyacinthus orientalis PISTILLATA p... 3 217 4.70E-14 1
G153 BG357222 BG357222 OV2_11_C11.b1_A002 Ovary 2 (OV2) S... 3 219 4.70E-14 1
G153 MDO291490 AJ291490 Malus domestica mRNA for Pistillat... 1 217 4.80E-14 1
G153 AI437956 AI437956 sa41 g11.y1 Gm-c1004 Glycine max cD... 3 219 4.80E-14 1
G153 GGN132209 AJ132209 Gnetum gnemon mRNA for putative MA... 1 233 4.80E-14 1
G153 MZEAGAMOU L81162 Zea maize AGAMOUS homologue mRNA, co... 2 234 4.90E-14 1
G153 BI098309 BI098309 IP1_30_G10.b1_A002 Immature pannic... 3 219 5.00E-14 1
G153 BE585982 BE585982 Est#7pT7_B09_b9_073 KSU wheat Fusa... 1 218 5.10E-14 1
G153 BI935126 BI935126 EST555015 tomato flower, anthesis ... 3 217 5.20E-14 1
G153 PHMADSBOX X81651 P.hybrida mRNA for MADS box containi... 2 226 5.20E-14 1
G153 BE431620 BE431620 EST336435 tomato breaker fruit, TI... 1 218 5.50E-14 1
G153 PGORFGAG2 Z46612 P.ginseng gag2 mRNA for agamous 2. 8... 3 231 5.60E-14 1
G153 BM412354 BM412354 EST586681 tomato breaker fruit Lye... 3 217 5.60E-14 1
G153 BG948060 BG948060 IP1_8_H04.b1_A002 Immature pannicl... 3 219 5.80E-14 1
G153 AI486684 AI486684 EST245006 tomato ovary, TAMU Lycop... 3 217 5.80E-14 1
G153 AF097747 AF097747 Cryptomeria japonica putative MADS... 1 217 5.90E-14 1
G153 BE317746 BE317746 NF054H08LF1 F1064 Developing leaf M... 1 220 5.90E-14 1
G153 CSA312773 AJ312773 Cucumis sativus mRNA for MADS1 pro... 3 234 6.20E-14 1
G153 BI933057 BI933057 EST552946 tomato flower, 8 mm to p... 3 217 6.30E-14 1
G153 AU029711 AU029711 AU029711 Rice panicle shorter than... 3 219 6.30E-14 1
G153 AB046596 AB046596 Cucumis sativus ERAF17 mRNA for pu... 3 216 6.40E-14 1
rf rf rr rr rr rr rr rr rr rr rr rr rr rr r rr rr rr rr rf rf rf rf rr rr rr rr rr rf rr rT rf rr rf rf
LU LU UJ UJ LU LU LU UJ UJ LU LU LU LU LU UJ LU LU LU LU UJ LU LU m LU m LU UJ UJ LU LU LU LU LU LU O O O O O O O O O O O O O O O O P P O O O O O O O O O O O O O O O O O O rf rr m co ι^ ι-- oo oo co c)o cΛ θ o o ^ c rr rr m m m m r-- rs. oo cΛ θ) σ) o p cM θJ co co rf m co' c cD ό cD cd d cd cd cD' r-.' r; r-.' r; r^ ι^ rs; rs; ι ; rs; r^ rs: r^ r^ r^ » |s. rs. rs. o 0) r-- rs. |s. D rs. cn -i- ^ o> o rs. rs. s. oO '>- rs. -- -^ r. rs. rs. oo oo r - rs. r. m rs. i-
τ- τ- w n θ ι- τ- τ- -
Figure imgf000171_0001
'- N cθ ι-
CN
Figure imgf000171_0002
52 CO C2 CO CO CO CO CO CO CO CO C CO C CO C CO CO C cr) M CO C CO CO CO CO CO CO CO CO CO CO CO CO CO m m m m m m m m m m m m m m m m m m m m m m m m in m Ln m m in m m m m m oδδδδδδδδδδδδδ δδδδδδδδδδδδδδδδδδδδδ mmmmmmmmmmm mmmmmmmmmmmmmmmmmmmmmmmm Table 2
G153 GI-1816459 DEFH 125 protein 1 267 1.10E-42 4
G153 GI-7489303 T17029 MADS-box transcription factor DEFH1... 1 267 1.10E-42 4
G153 GI-9964296 AF112149_1 MADS box protein 2 [Zea mays] 1 269 5.40E-42 5
G153 GI-1928874 MADS-box protein 1 243 2.70E-34 4
G153 GI-7488744 T09700 MADS-box protein - alfalfa (fragment) 1 243 2.70E-34 4
G153 GI-15824795 AF345911_1 MADS-box protein FDRMADS3 [Oryz... 1 241 9.90E-31 5
G153 GI-5295984 MADS box-like protein 1 237 1.70E-29 5
G153 GI-15081463 AF396746_1 MADS-box protein [Ipomoea batatas] 1 225 1.80E-28 3
G153 GI-939785 MADS box protein 1 241 2.50E-28 4
G153 GI-7446509 T03410 MADS box protein - maize 1 241 2.50E-28 4
G153 GI-12002141 AF112150_1 MADS box protein 3 [Zea mays] 1 244 1.30E-26 3
G153 GI-4204232 MADS-box protein 1 1 238 2.10E-26 3
G153 GI-167126 BAG1 1 229 2.90E-26 4
G153 GI-322650 A43484 probable transcription factor BAG1 ... 1 229 2.90E-26 4
G153 GI-399096 AG_BRANA AGAMOUS PROTEIN 1 229 2.90E-26 4
G153 GI-6606072 AF058698_1 MADS15 protein 1 237 5.60E-26 4
G153 GI-13446154 MADS-box transcription factor [Pisum sativum] 1 224 5.90E-26 4
G153 GI-13661024 MADS-box transcription factor [Pisum sativum] 1 224 5.90E-26 4
G153 GI-18252655 AF461740_1 MADS-box transcription factor M... 1 224 5.90E-26 4
G153 GI-9367313 MADS-box protein 8 [Hordeum vulgare] 1 235 8.70E-26 3
G153 GI-6634708 AF176782_1 MADS box transcription factor 1 233 1.10E-25 3
G153 GI-6731756 unnamed protein product 1 233 1.10E-25 3
G153 GI-4218162 MADS-box protein, GAGA2 1 234 1.40E-25 3
G153 GI-7592642 API-like MADS box protein [Oryza sativa] 1 237 2.20E-25 3
G153 GI-4204234 MADS-box protein 2 1 234 2.30E-25 3
G153 GI-5019431 putative MADS domain transcription factor ... 1 233 2.30E-25 4
G153 GI-1067169 floral binding protein number 7 1 226 2.90E-25 3
G153 GI-9956938 AF150931_1 MADS-domain protein PPM1 [Physc... 1 249 3.50E-25 3
G153 GI-9956940 AF150932_1 MADS-domain protein PPM1 [Physc... 1 249 3.50E-25 3
G153 GI-12019658 FDRMADS6 [Oryza sativa] 1 235 3.60E-25 3
G153 GI-3688589 MADS box transcription factor 1 234 3.60E-25 3
G153 GI-4218160 MADS-box protein, GAGA1 1 234 4.60E-25 3
G153 GI-1568513 fbp11 1 227 6.00E-25 3
G153 GI-6970411 MADS-box protein 1 233 9.40E-25 3
G153 GI-848999 agamous protein 1 234 1.20E-24 3
G153 GI-6467974 AF198175_1 MADS box protein DOMADS2 1 232 1.20E-24 3
CO CO « C CO CO CO rf rf C CO C P) C^ CO CM CM CM Cr) CO C CO CM C C C OI CM CM CM CO CO CO CO CO C
rr rr rr rr rr rr rr rf rf rr rr rr rr rr rr rr rr rr rr rr cO CO CO CO CO CO CO CO CO CN CM OJ CM CM CM CM C CM C C CM CM CM CM CM CM CM CM CM CM
O O O O P P P P P P P P O O O O O O O O O o o o o
0J C0 C0 m m C0 CM CM CM O 'r- ι- CΛ C0 C0 CD CD C0 τ- -- O O ->- '>-
Figure imgf000173_0001
cΛ m -r- oι m c) rr rr σ) c θ rr rr co m m ∞ oo rr oo rr rf C θ CD co cD cθ 'i- rs. r . rs. rs. co
CM -t- '- rr J rr J J CM CM ^ i- rr c C C CO CO CM CM C CM CM CM CM CM CO CO CO CO 't- CM CM CM CM J CM CM CM CM CM CM CM CM OJ CM CM CM CM CM CM CM CM CM OI CM OI OJ CM CM CM CM CM CM CM CM OJ OJ OJ OJ CM CM
Figure imgf000173_0002
oo co oo co co co co co co co oo oo co co co co co co co co m m m m m m m m m m m m m m m m m m m m m m m m m m m m m in m oooooooooooooooooooooooooooooooooooo m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m Table 2
15 G153 GI-9367309 MADS-box protein 5 [Hordeum vulgare] 1 215 2.80E-23 3
15 G153 GI-886405 MADS box protein 1 239 3.30E-23 3
15 G153 GI-2130078 S59480 MADS-box protein 3 - rice 1 239 3.30E-23 3
15 G153 GI-13161415 putative MADS-box protein [Oryza sativa] 1 239 3.30E-23 3
15 G153 GI-6468290 putative MADS domain transcription factor ... 1 223 3.40E-23 3
15 G153 GI-14518447 AF130118_1 MADS box protein [Capsicum annuum] 1 237 3.60E-23 2
15 G153 GI-1239963 MADS-box gene 1 222 4.40E-23 3
15 G153 GI-7446510 571756 MADS box protein DEFH72 - garden sn... 1 222 4.40E-23 3
15 G153 GI-4102113 NAP1-2 1 224 4.70E-23 2
15 G153 GI-1239959 MADS-box gene 1 221 5.50E-23 3
15 G153 GI-7446511 571757 MADS box protein DEFH200 - garden s... 1 221 5.50E-23 3
15 G153 GI-13384050 AF335236_1 MADS-box transcription factor F... 1 225 5.90E-23 2
15 G153 GI-19382 TDR4 1 234 7.40E-23 2
15 G153 GI-542035 S23730 TDR4 protein - tomato 1 234 7.40E-23 2
15 G153 GI-3912999 AGL8J.YCES FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 234 7.40E-23 2
15 G153 GI-887579 MADS box regulatory protein 1 231 7.40E-23 2
15 G153 GI-1046276 MADS box regulatory protein 1 231 7.40E-23 2
15 G153 GI-1364102 S57586 MADS box regulatory protein - Rumex... 1 231 7.40E-23 2
-4
Ui 15 G153 GI-5070140 AF068724_1 MADS-box protein MADS5 1 222 7.60E-23 2
15 G153 GI-2981131 AGAMOUS homolog 1 233 8.60E-23 3
15 G153 GI-8745072 MADS box protein [Betula pendula] 1 241 9.20E-23 2
15 G153 GI-2997613 CUM1 1 239 9.30E-23 2
15 G153 GI-4103346 agamous-like putative transcription factor 1 239 9.30E-23 2
15 G153 GI-7446520 T08039 MADS-box protein - cucumber 1 239 9.30E-23 2
15 G153 GI-9994504 FBP15 [Petunia x hybrida] 1 239 9.30E-23 2
15 G153 GI-1206003 putative MADS-box family transcription factor 1 224 9.60E-23 2
15 G153 GI-7446558 T09569 MADS box protein MADS1 - Monterey pine 1 224 9.60E-23 2
15 G153 GI-6683777 AF135962_1 CAGL2 1 223 9.60E-23 2
15 G153 GI-14279306 AF265562_1 MAD-box transcripion factor [V . 1 238 1.20E-22 2
15 G153 GI-313113 pMADS3 1 236 1.20E-22 2
15 G153 GI-478387 JQ2212 pMADS3 protein - garden petunia 1 236 1.20E-22 2
15 G153 GI-3913006 AG_PETHY AGAMOUS PROTEIN 1 236 1.20E-22 2
15 G153 GI-17827467 PMADS3 [Petunia x hybrida] 1 236 1.20E-22 2
15 G153 GI-1239961 MADS-box gene 1 222 1.20E-22 2
15 G153 GI-7446512 S78015 MADS box protein DEFH49 - garden sn... 1 222 1.20E-22 2
15 G153 GI-9956942 AF150933_1 MADS-domain protein PPM2 [Physc... 1 249 1.50E-22 2
Table 2
G153 GI-9956944 AF150934_1 MADS-domain protein PPM2 [Physc... 1 249 1.50E-22 2 G153 GI-8574457 AF072534_1 pepper MADS-box protein [Capsie... 1 233 1.50E-22 2 G153 GI-1483228 MADS3 protein 1 228 1.50E-22 2 G153 GI-5295982 MADS box-like protein 1 228 1.50E-22 2 G153 GI-13384060 AF335241_1 MADS-box transcription factor F... 1 221 1.60E-22 2 G153 GI-264223 promotes sex organ development=ple [Antirr... 1 230 1.80E-22 3 G153 GI-322801 A44343 promotes sex organ development prot... 1 230 1.80E-22 3 G153 GI-169254 transcription factor 1 229 1.80E-22 3 G153 GI-320595 JQ1689 floral binding protein 1 - garden p... 1 229 1.80E-22 3 G153 GI-729464 FBP1_PETHY FLORAL HOMEOTIC PROTEIN FBP1 (F.. 1 229 1.80E-22 3 G153 GI-2293892 unnamed protein product 1 229 1.80E-22 3 G153 GI-13177638 AF265554_1 transcription factor CMB [Cucum... 1 236 1.90E-22 2 G153 GI-18996775 AF462152_1 MADS-box transcription factor H... 1 227 2.00E-22 2 G153 GI-5070144 AF068726_1 MADS-box protein MADS2 1 223 2.00E-22 2 G153 GI-609253 Saapl 1 220 2.00E-22 2 G153 GI-1076477 S52236 Saapl protein - white mustard 1 220 2.00E-22 2 G153 GI-3913047 AP1_SINAL FLORAL HOMEOTIC PROTEIN APETALA1. 1 220 2.00E-22 2 G153 GI-16874557 MADS-box transcription factor DEFH28 [Anti... 1 229 2.50E-22 2 G153 GI-16052 SQUA 1 225 2.50E-22 2 G153 GI-82313 S20886 transcription factor squa - garden ... 1 225 2.50E-22 2 G153 GI-642593 amino acid feature: MADS box; codes for a ... 1 222 2.50E-22 2 G153 GI-7488599 T14457 MADS box protein homolog CAL - broc... 1 222 2.50E-22 2 G153 GI-642591 amino acid feature: K-box, bp 283..480; am... 1 222 2.50E-22 2 G153 GI-1561784 homeotic protein boiCAL 1 222 2.50E-22 2 G153 GI-6469345 DNA-binding protein 1 222 2.50E-22 2 G153 GI-7446539 T14456 MADS box protein homolog CAL - wild... 1 222 2.50E-22 2 G153 GI-431736 NAG1 1 236 3.10E-22 2 G153 GI-3913007 AG TOBAC AGAMOUS PROTEIN (NAG1) 1 236 3.10E-22 2 G153 GI-7446523 T03592 floral homeotic protein NAG1 - comm... 1 236 3.10E-22 2 G153 GI-6175371 AF091458_1 MADS box transcription factor M... 1 225 3.20E-22 2 G153 GI-5031217 AGAMOUS homolog 1 239 3.90E-22 2 G153 GI-2997615 CUM10 1 235 4.00E-22 2 G153 GI-7446521 T08040 MADS-box protein - cucumber 1 235 4.00E-22 2 G153 GI-457382 TAG1 1 232 4.00E-22 2 G153 GI-3913004 AGJ.YCES AGAMOUS PROTEIN (TAG1 ) 1 232 4.00E-22 2 G153 GI-7446522 T07185 floral homeotic protein TAG 1 - tomato 1 232 4.00E-22 2
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m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m Table 2
15 G153 GI-1076646 S46526 transcription factor - common tobacco 231 1.30E-21 2
15 G153 G1-8567991 AF068723_1 MADS-box protein MADS4 [Nicotia... 216 1.40E-21 2
15 G153 GI-3646340 MADS-box protein 228 1.70E-21 2
15 G153 GI-320596 JQ1690 floral binding protein 2 - garden p... 223 1.70E-21 2
15 G153 GI-1181186 transcription factor 223 1.70E-21 2
15 G153 GI-1345965 FBP2_PETHY FLORAL HOMEOTIC PROTEIN FBP2 (F., 223 1.70E-21 2
15 G153 GI-5070138 AF068722_1 MADS-box protein MADS3 223 1.70E-21 2
15 G153 GI-5777906 U78950_1 MADS-box protein 4 223 1.70E-21 2
15 G153 GI-3184054 MADS-box transcription factor 220 1.70E-21 2
15 G153 GI-7446516 T06543 MADS box protein - garden pea 220 1.70E-21 2
15 G153 GI-15808691 AF185574_1 transcription factor MAGL4 [Pop... 219 1.70E-21 2
15 G153 GI-2507629 putative MADS box transcription factor PrM... 206 2.00E-21 3
15 G153 GI-7446556 T10776 probable MADS box protein MADS6 - M... 206 2.00E-21 3
15 G153 GI-1321797 putative transcription factor 234 2.10E-21 2
15 G153 GI-4103344 agamous-like putative transcription factor 234 2.10E-21 2
15 G153 GI-7446562 T10185 MADS-box protein CUS1 - cucumber 234 2.10E-21 2
15 G153 GI-13448658 MADS box transcription factor [Ipomoea bat... 228 2.20E-21 2
15 G153 GI-951172 MADS box protein 238 2.70E-21 2
-4 βs 15 G153 GI-1001934 ZAG2 238 2.70E-21 2
15 G153 GI-508577 box protein 223 2.80E-21 2
15 G153 GI-1076739 S53306 box protein - rice 223 2.80E-21 2
15 G153 GI-10188329 unnamed protein product [Zea mays] 223 2.80E-21 2
15 G153 GI-11493807 MADS box protein MADS1 [Oryza sativa] 223 2.80E-21 2
15 G153 GI-695688 DAL2 protein 228 3.50E-21 2
15 G153 GI-4033710 AGAMOUS-like MADS-box transcription factor... 228 3.50E-21 2
15 G153 GI-4033721 AGAMOUS-like MADS-box transcriptional fact... 228 3.50E-21 2
15 G153 GI-4033723 AGAMOUS-like MADS-box transcriptional fact... 228 3.50E-21 2
15 G153 GI-7446536 T14847 MADS-box protein dal2 - Norway spruce 228 3.50E-21 2
15 G153 GI-4101710 MADS box transcription factor 228 3.50E-21 2
15 G153 GI-6840998 AF120097_1 DEF/GLO-like protein 227 3.90E-21 3
15 G153 GI-2252482 putative MADS domain transcription factor 223 3.90E-21 3
15 G153 GI-1001935 ZMM1 238 4.40E-21 2
15 G153 GI-1167914 MADS box protein 238 4.40E-21 2
15 G153 GI-7446525 T02261 MADS box protein - maize 238 4.40E-21 2
15 G153 G1-2981133 AGAMOUS homolog 233 4.40E-21 2
15 G153 GI-861081 agamous 231 4.40E-21 2
Table 2
15 G153 GI-3913005 AG_PANGI AGAMOUS PROTEIN (GAG2) 231 4.40E-21 2
15 G153 GI-13384052 AF335237_1 MADS-box transcription factor F... 228 4.40E-21 2
15 G153 GI-13384056 AF335239_1 MADS-box transcription factor F... 227 4.50E-21 2
15 G153 GI-3114588 MADS box protein 224 4.50E-21 2
15 G153 GI-10188331 unnamed protein product [Zea mays] 223 4.50E-21 2
15 G153 GI-16549058 putative MADS-domain transcription factor ... 230 5.60E-21 2
15 G153 GI-4103486 MADS box protein 228 5.60E-21 2
15 G153 GI-17432174 MADS-box protein [Lycopersicon esculentum] 217 5.80E-21 2
15 G153 GI-5051933 MADS-box protein FDRMADS8 215 5.80E-21 2
15 G153 GI-13274182 putative MADS-domain transcription factor ... 249 6.90E-21 2
15 G153 GI-6652756 MADS box protein 231 7.10E-21 2
15 G153 GI-4837612 farinelli protein 229 7.20E-21 2
15 G153 GI-1617211 MADS D 222 7.30E-21 2
15 G153 G1-3912986 AGL9_SINAL FLORAL HOMEOTIC PROTEIN AGL9 HO... 222 7.30E-21 2
15 G153 GI-7446553 T10467 MADS box protein D - white mustard 222 7.30E-21 2
15 G153 GI-1905930 MADS box protein 219 7.30E-21 2
15 G153 GI-7446519 T04335 MADS box protein - rice 219 7.30E-21 2 15 G153 GI-19358 TDR5 217 7.30E-21 2 15 G153 GI-19384 TDR5 217 7.30E-21 2
15 G153 GI-629674 S23728 TDR5 protein - tomato 217 7.30E-21 2
15 G153 GI-3913002 AGL9J.YCES FLORAL HOMEOTIC PROTEIN AGL9 HO... 217 7.30E-21 2
15 G153 GI-18057092 AC092697_3 putative transcription factor [... 215 7.40E-21 2
15 G153 GI-19871 NTGLOBOSA 229 9.10E-21 2
15 G153 GI-417063 GLOB_TOBAC FLORAL HOMEOTIC PROTEIN GLOBOSA 229 9.10E-21 2 5 G153 GI-448288 1916408A NTGLO gene [Nicotiana tabacum] 229 9.10E-21 2 5 G153 GI-486750 S35226 homeotic protein globosa homolog - ... 229 9.10E-21 2 5 G153 GI-1206005 putative MADS-box family transcription factor 223 9.20E-21 2 5 G153 GI-1702951 MADS-box family transcription factor 223 9.20E-21 2 5 G153 GI-7446546 T10486 MADS box protein - Canadian red pine 223 9.20E-21 2 5 G153 GI-7446559 T09571 MADS box protein MADS2 - Monterey pine 223 9.20E-21 2
15 G153 GI-3170502 APETALA3 homolog PnAP3-2 230 1.20E-20 2 5 G153 GI-5019460 putative MADS domain transcription factor ... 223 1.20E-20 2 5 G153 GI-3493647 transcription activator 218 1.20E-20 2 5 G153 GI-9367315 MADS-box protein 9 [Hordeum vulgare] 218 1.20E-20 2 5 G153 GI-13384046 AF335234_1 MADS-box transcription factor F... 228 1.50E-20 2
15 G153 GI-2463333 M79 protein 222 1.50E-20 2
Table 2
15 G153 GI-7446518 T04307 M79 protein - rice 1 222 1.50E-20 2
15 G153 GI-9367311 MADS-box protein 7 [Hordeum vulgare] 1 222 1.50E-20 2
15 G153 GI-4105097 MADS box protein 26 1 221 1.50E-20 2
15 G153 GI-1049022 transcription factor SaMADS A 1 217 1.50E-20 2
15 G153 GI-7446552 T10422 MADS box protein A - white mustard 1 217 1.50E-20 2
15 G153 GI-2529340 homologue; putative 1 234 1.90E-20 2
15 G153 GI-7446524 T01700 hypothetical protein - maize 1 234 1.90E-20 2
15 G153 GI-19380 TDR3 1 229 1.90E-20 2
15 G153 GI-542034 S23729 TDR3 protein - tomato 1 229 1.90E-20 2
15 G153 GI-1914838 MADS box protein 1 222 1.90E-20 2
15 G153 GI-5295964 EST D15657(C1032) corresponds to a region... 1 222 1.90E-20 2
15 G153 GI-7446542 T04168 MADS box protein - rice 1 222 1.90E-20 2
15 G153 GI-1905944 MADS box transcription factor SbMADSI 1 220 1.90E-20 2
15 G153 GI-7446545 T14801 MADS box protein MADS1 - sorghum 1 220 1.90E-20 2
15 G153 GI-7544096 pMADS4 [Petunia x hybrida] 1 218 1.90E-20 2
15 G153 GI-13384048 AF335235_1 MADS-box transcription factor F... 1 218 1.90E-20 2
15 G153 GI-3646322 MADS-box protein 1 208 2.00E-20 2
15 G153 GI-13274180 putative MADS-domain transcription factor ... 1 245 2.30E-20 2
-4
90 15 G153 GI-18076209 putative MADS-domain transcription factor ... 1 240 2.30E-20 2
15 G153 GI-5019429 putative MADS domain transcription factor ... 1 231 2.40E-20 2
15 G153 GI-2735764 MADS transcriptional factor; STMADS16 1 227 2.40E-20 2
15 G153 GI-7446547 T06995 probable MADS box transcription fac... 1 227 2.40E-20 2
15 G153 GI-16549070 putative MADS-domain transcription factor ... 1 223 2.40E-20 2
15 G153 GI-939779 MADS box protein 1 218 2.40E-20 2
15 G153 GI-7446514 T03398 MADS box protein - maize 1 218 2.40E-20 2
15 G153 GI-13958339 AF372840_1 madsl [Poa annua] 1 218 2.40E-20 2
15 G153 GI-6580943 AF158541_1 MADS-box transcription factor 1 218 2.40E-20 2
15 G153 GI-6580945 AF158542_1 MADS-box transcription factor 1 218 2.40E-20 2
15 G153 GI-6580947 AF158543_1 MADS-box transcription factor 1 215 2.70E-20 3
15 G153 GI-3114586 MADS box protein 1 234 3.00E-20 2
15 G153 GI-10880315 putative MADS-domain transcription factor ... 1 233 3.00E-20 2
15 G153 GI-6468286 putative MADS domain transcription factor ... 1 224 3.10E-20 2
15 G153 GI-695690 DAL3 protein 1 214 3.10E-20 2
15 G153 GI-7484434 T14848 MADS-box protein dal3 - Norway spruce 1 214 3.10E-20 2
15 G153 GI-18650789 AF234617_1 MADS-box transcription factor [... 1 235 3.80E-20 2
15 G153 GI-13384054 AF335238_1 MADS-box transcription factor F... 1 219 3.90E-20 2
Table 2
15 G153 GI-3688591 MADS box transcription factor 218 3.90E-20 2
15 G153 GI-2507623 putative MADS box transcription factor PrM... 228 4.90E-20 2
15 G153 GI-7446554 T10751 MADS-box protein MADS9 - Monterey pine 228 4.90E-20 2
15 G153 GI-5295980 MADS box-like protein 219 5.00E-20 2
15 G153 GI-695317 MADS box protein 218 5.00E-20 2
15 G153 G1-2286109 MADS box protein 218 5.00E-20 2
15 G153 GI-2286113 MADS box protein 218 5.00E-20 2
15 G153 GI-3023536 CMB1_DIACA MADS BOX PROTEIN CMB1 218 5.00E-20 2
15 G153 GI-7446517 T04167 MADS box protein - rice 218 5.00E-20 2
15 G153 GI-7446544 T04170 MADS box protein - rice 218 5.00E-20 2
15 G153 GI-7446563 T10714 MADS-box protein CMB1 - clove pink 218 5.00E-20 2
15 G153 GI-12597207 putative MADS-box protein [Cucumis sativus] 216 5.00E-20 2
15 G153 GI-10803404 MADS box protein [Gerbera hybrida] 218 6.40E-20 2
15 G153 GI-4322475 putative MADS box transcription factor ETL 216 6.40E-20 2
15 G153 GI-2286111 MADS box protein 215 8.10E-20 2
15 G153 GI-7446543 T04169 MADS box protein - rice 215 8.10E-20 2
15 G153 GI-6580949 AF158544_1 MADS-box transcription factor 215 8.70E-20 3
15 G153 GI-5295978 MADS box-like protein 193 9.30E-20 3
-4 sO 15 G153 G1-4406132 MADS box protein 216 1.00E-19 2
15 G153 GI-16417764 AF424549_1 MADS-box protein SPW1 [Oryza sa... 216 1.00E-19 2
15 G153 GI-4885034 AF134114_1 PISTILLATA protein homolog HPH 215 1.00E-19 2
15 G153 GI-15077026 AF286649_1 transcription factor CMB1 [Cucu... 210 1.00E-19 2
15 G153 GI-3986689 MADS box protein 230 1.30E-19 2
15 G153 GI-3688593 MADS box transcription factor 220 1.30E-19 2
15 G153 GI-939781 MADS box protein 214 1.30E-19 2
15 G153 GI-7446515 T03408 MADS box protein - maize 214 1.30E-19 2
15 G153 GI-4433623 MADS-box transcription factor 213 1.30E-19 2
15 G153 GI-6467972 AF198174_1 MADS box protein DOMADS1 213 1.30E-19 2
15 G153 GI-22665 GP (green petal) 207 1.40E-19 3
15 G153 GI-322773 S31693 transcription factor gp - garden pe... 207 1.40E-19 3
15 G153 GI-729974 MAD1_PETHY FLORAL HOMEOTIC PROTEIN PMADS1 207 1.40E-19 3
15 G153 GI-3337237 putative MADS box transcription factor PrM... 205 1.70E-19 2
15 G153 GI-7446557 T10778 probable MADS box protein MADS8 - M... 205 1.70E-19 2
15 G153 GI-13384068 AF335245_1 MADS-box transcription factor F... 232 2.10E-19 2
15 G153 GI-15667638 AF097746_1 putative MADS-box family transc... 230 2.10E-19 2
15 G153 GI-3170464 APETALA3 homolog PnPI-1 225 2.10E-19 2
Table 2
G153 GI-2507627 putative MADS box transcription factor PrM... 1 224 2.10E-19 2
G153 GI-7446555 T10767 probable MADS box protein MADS5 - M... 1 224 2.10E-19 2
G153 GI-16024 globosa 1 223 2.10E-19 2
G153 GI-282981 S28062 homeotic protein globosa - garden s... 1 223 2.10E-19 2
G153 GI-417062 GLOB_ANTMA FLORAL HOMEOTIC PROTEIN GLOBOSA 1 223 2.10E-19 2
G153 GI-7328575 AF181479_1 MADS-box DNA binding protein [Z... 1 217 2.70E-19 2
G153 GI-12964064 MADS box transcription factor [Oryza sativa] 1 219 3.40E-19 2
G153 GI-6635740 AF207699_1 agamous-like MADS box protein O... 1 211 3.50E-19 2
G153 GI-8745070 MADS box protein [Betula pendula] 1 210 3.50E-19 2
G153 GI-4218169 MADs-box protein, GDEF1 1 209 3.50E-19 2
G153 GI-439239 MADS-box protein 1 205 4.50E-19 2
G153 GI-632112 S40405 MADS-box protein - Aranda deborah 1 205 4.50E-19 2
G153 GI-3912996 AGL9_ARADE FLORAL HOMEOTIC PROTEIN AGL9 HO... 1 205 4.50E-19 2
G153 GI-13442962 AF095646_1 MADS box protein nmads3 [Oryza ... 1 189 4.90E-19 3
G153 GI-4218173 MADS-box protein, GGL01 1 215 5.60E-19 2
G153 GI-12002139 AF112148_1 MADS box protein 1 [Zea mays] 1 215 5.60E-19 2
G153 GI-5295990 MADS box-like protein 1 214 5.60E-19 2
G153 GI-6467976 AF198176_1 MADS box protein DOMADS3 1 213 5.60E-19 2
G153 GI-13448660 MADS box transcription factor [Ipomoea bat... 1 212 5.60E-19 2
G153 GI-3646336 MdMADS9 1 192 5.80E-19 2
G153 GI-22667 PMADS2 1 217 7.00E-19 2
G153 GI-322775 S31707 transcription factor pmads2 - garde... 1 217 7.00E-19 2
G153 GI-454265 FBP3 1 217 7.00E-19 2
G153 GI-729976 MAD2_PETHY FLORAL HOMEOTIC PROTEIN PMADS 2 1 217 7.00E-19 2
G153 GI-2129971 S60288 FBP3 protein - garden petunia 1 217 7.00E-19 2
G153 GI-12666533 Pistillata MADS-box protein [Malus x domes... 1 217 7.00E-19 2
G153 GI-12666535 Pistillata MADS-box protein [Malus x domes... 1 217 7.00E-19 2
G153 GI-4033725 AGAMOUS-like MADS-box transcriptional fact... 1 206 7.20E-19 2
G153 GI-2507625 putative MADS box transcription factor PrM... 1 200 7.30E-19 2
G153 GI-7446564 T10764 probable MADS box protein MADS4 - M... 1 200 7.30E-19 2
G153 GI-5295986 MADS box-like protein 1 230 8.70E-19 2
G153 GI-2735766 MADS transcriptional factor; STMADS11 1 216 9.00E-19 2
G153 GI-7446550 T06996 MADS-box transcription factor MADS1... 1 216 9.00E-19 2
G153 GI-4096982 AGAMOUS protein 1 228 1.10E-18 2
G153 GI-2827300 MADS-box protein NMH 7 1 217 1.40E-18 2
G153 GI-7446532 T09335 MADS-box protein NMH 7 - alfalfa 1 217 1.40E-18 2
Table 2
G153 GI-1870206 MADS-box protein 1 212 1.50E-18 2
G153 GI-2293894 unnamed protein product 1 194 1.50E-18 2
G153 GI-6970417 MADS-box protein 1 228 1.80E-18 2
G153 GI-2981614 transcription factor 1 217 1.80E-18 2
G153 GI-602902 SLM2 1 203 1.90E-18 2
G153 GI-2961437 MADS box protein 1 238 2.40E-18 1
G153 GI-3646326 MADS-box protein 1 238 2.40E-18 1
G153 GI-7446535 T03902 MADS4 box protein - rice 1 238 2.40E-18 1
G153 GI-8216957 putative transcription factor [Cucumis sat... 1 200 2.40E-18 2
G153 GI-9367232 MADS box protein 1 [Hordeum vulgare] 1 211 3.00E-18 2
G153 GI-9367234 MADS-box protein 1 -2 [Hordeum vulgare] 1 211 3.00E-18 2
G153 GI-8163950 AF230704_1 MADS box transcription factor T... 1 206 3.00E-18 2
G153 GI-790637 AGL15 1 236 3.90E-18 1
G153 GI-3831486 AG15_BRANA FLORAL HOMEOTIC PROTEIN AGL15 1 236 3.90E-18 1
G153 GI-7446560 T07867 MADS box protein AGL15 (type 1) - rape 1 236 3.90E-18 1
G153 GI-13442960 AF095645_1 MADS box protein nmadsl [Oryza ... 1 224 4.70E-18 2
G153 GI-15022157 MADS box protein-like protein NGL9 [Medica... 1 212 4.80E-18 2
G153 GI-6580939 AF158539_1 MADS-box transcription factor 1 212 4.80E-18 2
G153 GI-6580941 AF158540_1 MADS-box transcription factor 1 212 4.80E-18 2
G153 GI-1370276 MADS-box protein 1 207 4.90E-18 2
G153 GI-8163966 AF230712_1 MADS box containing protein PI ... 1 204 4.90E-18 2
G153 GI-5616513 AF168468_1 agamous protein 1 235 5.00E-18 1
G153 GI-6970415 MADS-box protein 1 235 5.00E-18 1
G153 GI-15667640 AF097747_1 putative MADS-box family transc... 1 217 7.70E-18 2
G153 GI-1362199 555773 homeotic protein ZAG2 - maize (frag... 1 233 8.10E-18 1
G153 GI-5051935 MADS-box protein FDRMADS1 1 190 8.20E-18 2
G153 GI-6465895 Apetala 1 protein 1 182 8.30E-18 2
G153 GI-6468288 putative MADS domain transcription factor ... 1 188 8.40E-18 3
G153 GI-16973294 B-type MADS box protein [Malus x domestica] 1 194 1.00E-17 2
G153 GI-1362200 555774 homeotic protein ZMM1 - maize (frag... 1 232 1.00E-17 1
G153 GI-7677036 FDRMADS7 [Oryza sativa] 1 182 1.10E-17 2
G153 GI-19388 TDR8 1 220 1.20E-17 2
G153 GI-542036 S23732 TDR8 protein - tomato 1 220 1.20E-17 2
G153 GI-1418272 AGL15 type 2 1 219 1.20E-17 2
G153 GI-7446561 T07869 MADS box protein AGL15 (type 2) - rape 1 219 1.20E-17 2
G153 GI-13384062 AF335242_1 MADS-box transcription factor F... 1 231 1.30E-17 1
Table 2
G153 GI-16973298 C-type MADS box protein [Malus x domestica] 1 231 1.30E-17 1
G153 GI-9857312 MADS-box protein [Rosa rugosa] 1 205 1.60E-17 2
G153 GI-8163952 AF230705_1 MADS box transcription factor A... 1 199 1.60E-17 2
G153 GI-431226 deficiens analogue 1 203 2.10E-17 2
G153 GI-511065 deficiens analogue 1 203 2.10E-17 2
G153 GI-7446533 T07410 MADS box protein homolog DEF2 - potato 1 203 2.10E-17 2
G153 GI-7446549 T07066 MADS-box protein homolog DEF4 - potato 1 203 2.10E-17 2
G153 GI-309576 homologue of Arabidopsis Agamous-like gene 1 201 2.10E-17 2
G153 GI-542192 PQ0770 floral homeotic protein ZAG2 - maiz... 1 201 2.10E-17 2
G153 GI-10835358 PTD protein [Populus balsamifera subsp. tr... 1 203 2.60E-17 2
G153 GI-6970413 MADS-box protein 1 228 2.80E-17 1
G153 GI-17933452 MADS-box protein [Brassica napus] 1 207 3.30E-17 2
G153 GI-3253147 CMADS1 1 232 4.70E-17 3
G153 GI-17933454 MADS-box protein [Brassica napus] 1 211 6.80E-17 2
G153 GI-13384064 AF335243_1 MADS-box transcription factor F... 1 205 6.90E-17 2
G153 GI-10880313 putative MADS-domain transcription factor ... 1 224 7.40E-17 1
G153 GI-16018 DEF A protein 1 203 8.70E-17 2
G153 GI-16020 deficiens 1 203 8.70E-17 2
G153 GI-100479 S12378 defA-1 protein - garden snapdragon 1 203 8.70E-17 2
G153 GI-100483 S19232 gene deficiens protein - garden sna... 1 203 8.70E-17 2
G153 GI-118426 DEFA_ANTMA FLORAL HOMEOTIC PROTEIN DEFICIENS 1 203 8.70E-17 2
G153 GI-1561782 homeotic protein boi1AP3 1 203 8.70E-17 2
G153 GI-6841082 AF124814_1 APETALA3 1 203 8.70E-17 2
G153 GI-19386 TDR6 1 189 9.00E-17 2
G153 GI-481512 S38778 TDR6 protein - tomato (fragment) 1 189 9.00E-17 2
G153 GI-542029 S23731 TDR6 protein - tomato (fragment) 1 189 9.00E-17 2
G153 GI-16549083 putative MADS-domain transcription factor ... 1 175 9.30E-17 2
G153 GI-1362203 S55938 homeotic protein ZMM4 - maize (frag... 1 223 9.40E-17 1
G153 GI-602906 SLM4 1 221 1.50E-16 1
G153 GI-1561786 homeotic protein boi2AP3 1 205 1.80E-16 2
G153 GI-7446540 T14473 MADS box protein 2AP3 - broccoli 1 205 1.80E-16 2
G153 GI-16549081 putative MADS-domain transcription factor ... 1 185 1.90E-16 2
G153 GI-3851331 putative MADS-domain transcription factor 1 177 1.90E-16 2
G153 GI-1362196 S55772 homeotic protein TMZ1-I15a - maize ... 1 220 2.00E-16 1
G153 GI-1362198 S55768 homeotic protein ZAG1 - maize (frag... 1 220 2.00E-16 1
G153 GI-17933458 MADS-box protein [Brassica napus] 1 198 2.30E-16 2
Table 2
15 G153 GI-6465897 Apetala 1 protein 1 177 3.10E-16 2
15 G153 GI-3892652 putative MADS-domain transcription factor 1 175 3.10E-16 2
15 G153 GI-5764249 AF147240_1 ASAPETALA3 TM6-A 1 159 3.10E-16 3
15 G153 GI-3253149 CMADS2 1 218 3.20E-16 1
15 G153 GI-10880311 putative MADS-domain transcription factor ... 1 200 3.70E-16 2
15 G153 GI-16549060 putative MADS-domain transcription factor ... 1 179 3.90E-16 2
15 G153 GI-4885036 AF134115_1 PISTILLATA protein homolog HPI2 1 217 4.10E-16 1
15 G153 GI-5230654 MADS-box protein 1 204 4.70E-16 2
15 G153 GI-2055376 MADS box protein 1 180 4.90E-16 2
15 G153 GI-5764257 AF147244-.1 ASAPETALA3/TM6-B 1 159 5.00E-16 3
15 G153 GI-5764265 AF147248_1 ASAPETALA3/TM6-B 1 158 5.00E-16 3
15 G153 GI-16549062 putative MADS-domain transcription factor ... 1 179 6.20E-16 2
15 G153 GI-5764245 AF147238_1 ASAPETALA3/TM6-A 1 159 6.40E-16 3
15 G153 GI-1006768 ZMM2 1 190 7.80E-16 2
15 G153 GI-7446528 T04388 AGAMOUS-like MADS-box protein ZMM2 ... 1 190 7.80E-16 2
15 G153 GI-8163962 AF230710_1 MADS box containing protein PI ... 1 187 7.80E-16 2
15 G153 GI-5051937 MADS-box protein FDRMADS2 1 178 7.90E-16 2 - 15 G153 GI-1621333 homeotic protein bobap3 1 198 1.20E-15 2
00 w 15 G153 GI-7446541 T14474 MADS box protein ap3 - broccoli 1 198 1.20E-15 2
15 G153 GI-6465899 Apetala 1 protein 1 185 1.30E-15 2
15 G153 GI-10880319 putative MADS-domain transcription factor ... 1 171 1.70E-15 2
15 G153 GI-3170468 PISTILLATA homolog DePI-1 1 170 1.70E-15 2
15 G153 GI-14041687 putative agamous protein [Juglans regia] 1 158 1.70E-15 2
15 G153 GI-1944532 homeotic protein 1 211 1.80E-15 1
15 G153 GI-3253151 MADS-box protein 1 211 1.80E-15 1
15 G153 GI-793904 ZEM2 1 210 2.30E-15 1
15 G153 GI-793906 ZEM3 1 210 2.30E-15 1
15 G153 GI-1362195 555770 homeotic protein TMZ1-I20a - maize ... 1 210 2.30E-15 1
15 G153 GI-1362197 555771 homeotic protein TMZ1-I5a - maize (... 1 210 2.30E-15 1
15 G153 GI-1362209 S56755 ZEM2 protein - maize (fragment) 1 210 2.30E-15 1
15 G153 GI-5764271 AF147251_1 ASAPETALA3 TM6-B 1 159 2.60E-15 3
15 G153 GI-17933450 MADS-box protein [Brassica napus] 1 209 2.90E-15 1
15 G153 GI-3170512 APETALA3 homolog PhAP3 1 183 3.30E-15 2
15 G153 GI-3851333 putative MADS-domain transcription factor 1 174 4.30E-15 2
15 G153 GI-793902 ZEM1 1 205 7.80E-15 1
15 G153 GI-887575 MADS box regulatory protein 1 205 7.80E-15 1
Table 2
G153 GI-1046272 MADS box regulatory protein 1 205 7.80E-15 1 G153 GI-1076827 S54209 ZEM1 protein - maize (fragment) 1 205 7.80E-15 1 G153 GI-1362208 S56754 ZEM1 protein - maize (fragment) 1 205 7.80E-15 1 G153 GI-2507631 putative MADS box transcription factor PrM... 1 204 9.90E-15 1 G153 GI-7484486 T10777 probable MADS box protein MADS7 - M- 1 204 9.90E-15 1 G153 GI-6092007 GpMADSI 1 190 1.10E-14 2 G153 GI-2981610 transcription factor 1 203 1.30E-14 1 G153 GI-695319 MADS box protein 1 202 1.60E-14 1 G153 GI-722400 MADS box protein 1 202 1.60E-14 1 G153 GI-3023537 CMB2_DIACA MADS BOX PROTEIN CMB2 1 202 1.60E-14 1 G153 GI-7446538 T10715 MADS-box protein CMB2 - clove pink 1 202 1.60E-14 1 G153 GI-16549066 putative MADS-domain transcription factor ... 1 202 1.60E-14 1 G153 GI-17933456 MADS-box protein [Brassica napus] 1 202 1.60E-14 1 G153 GI-5764255 AF147243_1 ASAPETALA3/TM6-B 1 159 1.80E-14 2 G153 GI-5764269 AF147250_1 ASAPETALA3/TM6-B 1 159 1.80E-14 2 G153 GI-939783 MADS box protein 1 201 2.10E-14 1 G153 GI-7489788 T03409 MADS box protein - maize (fragment) 1 201 2.10E-14 1 G153 GI-3170478 PISTILLATA homolog DaPI-1 1 171 2.90E-14 2 G153 GI-5764275 AF147253_1 ASAPETALA3/TM6 1 160 3.00E-14 2 G153 GI-4218171 MADS-box protein, GDEF2 1 199 3.40E-14 1 G153 GI-5825623 AF180364_1 DEFICIENS homolog DEF1 1 199 3.40E-14 1 G153 GI-5825625 AF180365_1 DEFICIENS homolog DEF2 1 199 3.40E-14 1 G153 GI-6010661 AF181664_1 squamosa/apetalal homolog 1 182 3.60E-14 2 G153 GI-3170498 APETALA3 homolog PcAP3 1 176 3.70E-14 2 G153 GI-5764237 AF147234_1 ASAPETALA3/TM6-A 1 159 3.80E-14 2 G153 GI-5764241 AF147236_1 ASAPETALA3/TM6-A 1 159 3.80E-14 2 G153 GI-5764251 AF147241_1 ASAPETALA3/TM6-A 1 159 3.80E-14 2 G153 GI-5764253 AF147242_1 ASAPETALA3/TM6-A 1 159 3.80E-14 2 G153 GI-5764267 AF147249_1 ASAPETALA3/TM6-B 1 159 3.80E-14 2 G153 GI-5764277 AF147254_1 ASAPETALA3 M6 1 159 3.80E-14 2 G153 GI-5764279 AF147255_1 ASAPETALA3/TM6 1 159 3.80E-14 2 G153 GI-5764281 AF147256_1 ASAPETALA3/TM6 1 159 3.80E-14 2 G860 AI974336 AI974336 T110185e KVO Medicago truncatula c... 2 262 1.20E-37 3 G860 AMDEFH125 Y10750 A.majus mRNA for DEFH125 protein. 8/.. 1 258 1.30E-37 3 G860 AX085151 AX085151 Sequence 1 from Patent WO0112798. 1 261 5.00E-37 4 G860 AX085348 AX085348 Sequence 1 from Patent WO0112799. 1 261 5.00E-37 4
Table 2
17 G860 AF112149 AF112149 Zea mays MADS box protein 2 (mads2... 1 261 5.00E-37 4
17 G860 OSJN00003 AL606460 Oryza sativa chromosome 4 clone OS... 1 265 3.90E-36 4
17 G860 MSU91964 U91964 Medicago sativa MADS-box protein mRN... 1 241 1.30E-35 3
17 G860 AP004766 AP004766 Oryza sativa (japonica cultivar-gr... 1 269 3.60E-35 3
17 G860 AP003868 AP003868 Oryza sativa chromosome 8 clone OJ... 3 276 2.30E-34 4
17 G860 AL389596 AL389596 MtBC56A01 F1 MtBC Medicago truncatu... 2 228 8.40E-34 3
17 G860 AW218280 AW218280 EST303461 tomato radicle, 5 d post... 3 261 9.00E-31 2
17 G860 BM405213 BM405213 EST579540 potato roots Solanum tub... 2 262 2.90E-29 2
17 G860 AX085162 AX085162 Sequence 12 from Patent WO0112798.... 1 266 2.40E-28 2
17 G860 AX085359 AX085359 Sequence 12 from Patent WO0112799.... 1 266 2.40E-28 2
17 G860 AW706936 AW706936 sk08d08.y1 Gm-c1023 Glycine max cD... 2 260 3.10E-28 2
17 G860 AP004526 AP004526 Lotus japonicus genomic DNA, chrom... -2 269 4.40E-28 3
17 G860 BG592879 BG592879 EST491557 cSTS Solanum tuberosum c... 3 264 2.90E-27 2
17 G860 BE596704 BE596704 PI1_58_F04.b1_A002 Pathogen induce... 1 268 1.90E-26 2
17 G860 BE354988 BE354988 DG1_10_H09.b1_A002 Dark Grown 1 (D... 2 251 5.80E-25 2
17 G860 BE445262 BE445262 WHE1133_A05_A09ZS Wheat etiolated ... 2 248 4.00E-24 2
17 G860 AF198175 AF198175 Dendrobium grex Madame Thong-IN MA... 2 210 3.70E-21 2
17 G860 AQ917367 AQ917367 T233384b Medicago truncatula BAC I... 2 281 6.10E-21 1
90 Ul 17 G860 AF396746 AF396746 Ipomoea batatas MADS-box protein (... 1 219 7.00E-21 2
17 G860 BE034098 BE034098 MG05B05 MG Mesembryanthemum crysta... 1 205 2.70E-20 2
17 G860 BM268642 BM268642 MEST398-C12.univ ISUM5-RN Zea mays... -3 273 3.60E-20 1
17 G860 BM334225 BM334225 MEST134-C05.T3 ISUM5-RN Zea mays c... -1 273 4.20E-20 1
17 G860 BM323459 BM323459 PIC1_19_E07.b1_A002 Pathogen-infec... 3 268 1.60E-19 1
17 G860 AX027353 AX027353 Sequence 1 from Patent WO0037488. ... 2 231 1.90E-19 2
17 G860 BE341755 BE341755 EST394587 potato stolon, Cornell U... 3 264 5.00E-19 1
17 G860 MDAJ763 AJ000763 Malus domestica mRNA for MADS-box ... 2 230 5.80E-19 2
17 G860 BE034403 BE034403 MH04D03 MH Mesembryanthemum crysta... 3 258 1.10E-18 1
17 G860 BG651806 BG651806 sad61c04.y1 Gm-c1051 Glycine max c... 3 260 1.20E-18 1
17 G860 AP004616 AP004616 Oryza sativa chromosome 6 clone PO... -2 245 2.60E-18 2
17 G860 OSA011675 AJ011675 Oryza sativa mRNA for MADS-box pro... 2 212 3.50E-18 2
17 G860 AP003908 AP003908 Oryza sativa chromosome 8 clone OJ... -1 276 3.60E-18 1
17 G860 ZMA292959 AJ292959 Zea mays mRNA for putative MADS-do... 1 247 1.40E-17 1
Figure imgf000186_0001
17 G860 AB022665 AB022665 Gnetum parvifolium GpMADS3 mRNA, c... 2 221 1.40E-17 2
17 G860 AF091458 AF091458 Oryza sativa MADS box transcriptio... 3 212 1.60E-17 2
17 G860 GGN132215 AJ132215 Gnetum gnemon mRNA for putative MA... 1 221 1.80E-17 2
17 G860 AW330598 AW330598 707037C10.x1 707 - Mixed adult tis... 2 247 2.30E-17 1
i- CM CM CM i- i- CM i- T- CM CM CM CM CM -'- f- 'i- CM
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< m < CQ CQ < < < < CQ < < < CQ < 2 < CQ CQ CQ CQ CQ < CL Z C0 < CQ < CQ CQ < αj CQ C0
O P P O P O O O O P O P P O O O O O O O O O O O O O O O O O O O O O O O O CO CO CO CO CO CO CO CD CD P CO CO CO CO P CD CO CO CO CO CO P CO CD CO CO CO P CD CD m p CO CO CO
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000000000000000000000000000000000000 Table 2
17 G860 AI794686 AI794686 sb67f02.y1 Gm-c1019 Glycine max cD... 3 229 1.90E-15 1
17 G860 AQ330858 AQ330858 nbxb0048E10f CUGI Rice BAC Library... 2 229 2.00E-15 1
17 G860 BE498814 BE498814 WHE0966_E12_J24ZS Wheat pre-anthes... 3 230 2.00E-15 1
17 G860 D22380 D22380 RICC10934A Rice callus Oryza sativa ... 3 231 2.00E-15 1
17 G860 BE430838 BE430838 SUN006.D03F991220 ITEC SUN Wheat c... 1 228 2.00E-15 1
17 G860 BG274182 BG274182 WHE2230_E05_I10ZS Aegilops speltoi... 3 230 2.10E-15 1
17 G860 BG274504 BG274504 WHE2237_G06_M11ZS Aegilops speltoi... 1 230 2.10E-15 1
17 G860 BE454527 BE454527 HVSMEh0094C05f Hordeum vulgare 5-4... 1 227 2.10E-15 1
17 G860 C75937 C75937 C75937 Rice callus Oryza sativa cDNA... 2 231 2.10E-15 1
17 G860 BE454695 BE454695 HVSMEh0095C18f Hordeum vulgare 5-4... 3 227 2.20E-15 1
17 G860 BE456120 BE456120 HVSMEg0019N18f Hordeum vulgare pre... 3 227 2.20E-15 1
17 G860 BF273723 BF273723 GA Eb0018M24f Gossypium arboreum ... 1 229 2.20E-15 1
17 G860 BM134188 BM134188 NXLV_017_H01_F NXLV (Nsf Xylem Lat... 2 229 2.30E-15 1
17 G860 BJ195968 BJ195968 BJ195968 normalized full length cD... 2 229 2.30E-15 1
17 G860 BG414586 BG414586 HVSMEk0002P16f Hordeum vulgare tes... 3 227 2.40E-15 1
17 G860 BE637049 BE637049 WHE1821-1824_G05_G05ZS Secale cere... 1 230 2.50E-15 1
17 G860 BG442607 BG442607 GA Ea0017K04f Gossypium arboreum ... 3 228 2.50E-15 1 s 17 G860 BF201770 BF201770 WHE1765_E01_J01ZS Wheat pre-anthes... 3 230 2.60E-15 1 oc 1? G860 AI728519 Al 728519 BNLG Hi 10938 Six-day Cotton fiber G... 2 228 2.60E-15 1
17 G860 AF097746 AF097746 Cryptomeria japonica putative MADS... 3 226 2.70E-15 1
17 G860 AI898432 AI898432 EST267875 tomato ovary, TAMU Lycop... 1 227 2.70E-15 1
17 G860 AL506480 AL506480 AL506480 Hordeum vulgare Barke dev... 1 227 2.70E-15 1
17 G860 AL506894 AL506894 AL506894 Hordeum vulgare Barke dev... 2 227 2.70E-15 1
17 G860 AL507763 AL507763 AL507763 Hordeum vulgare Barke dev... 1 227 2.70E-15 1
17 G860 AL508019 AL508019 AL508019 Hordeum vulgare Barke dev... 1 227 2.70E-15 1
17 G860 AL509053 AL509053 AL509053 Hordeum vulgare Barke dev... 2 227 2.70E-15 1
17 G860 AL506475 AL506475 AL506475 Hordeum vulgare Barke dev... 1 227 2.80E-15 1
17 G860 AI487420 AI487420 EST245742 tomato ovary, TAMU Lycop... 1 227 2.90E-15 1
17 G860 BG412085 BG412085 OV2_37_G09.b1_A002 Ovary 2 (OV2) S... 3 228 3.00E-15 1
17 G860 BM359874 BM359874 GA Ea0024l13r Gossypium arboreum ... 2 228 3.30E-15 1
17 G860 AW289098 AW289098 707008H08.X3 707 - Mixed adult tis... 1 228 3.50E-15 1
17 G860 OSJN00086 AL606688 Oryza sativa chromosome 4 clone OS... 3 223 3.70E-15 2
17 G860 BG367358 BG367358 HVSMEι'0011 P20f Hordeum vulgare 20 ... 2 227 3.70E-15 1
17 G860 ZMA292961 AJ292961 Zea mays mRNA for putative MADS-do... 3 239 3.80E-15 1
17 G860 AF072534 AF072534 Capsicum annuum pepper MADS-box pr... 1 225 3.90E-15 1
17 G860 BH712678 BH712678 BOHV069TF BO_2_3_KB Brassica olera... -2 225 4.00E-15 1
Table 2
17 G860 BG446848 BG446848 GA Eb0039l21f Gossypium arboreum ... 2 226 4.10E-15
17 G860 AC092697 AC092697 Oryza sativa chromosome 10 BAC OSJ... 1 225 4.20E-15
17 G860 AF052855 AF052855 Papaver nudicaule APETALA3 homolog... 1 224 4.40E-15
17 G860 BM442374 BM442374 EBan01_SQ003_A06_R IGF Barley EBan... 2 227 4.50E-15
17 G860 ZMA271208 AJ271208 Zea mays mRNA for putative MADS-do... 3 236 5.00E-15
17 G860 BG054384 BG054384 OV2_3_G05.b1_A002 Ovary 2 (OV2) So... 3 228 5.10E-15
17 G860 BG417142 BG417142 HVSMEk0016H10f Hordeum vulgare tes... 1 226 5.20E-15
17 G860 BE918068 BE918068 OV1_1_E07.b1_A002 Ovary 1 (OV1) So... 2 228 5.20E-15
17 G860 BI311095 BI311095 EST5312845 GESD Medicago truncatul... 2 224 5.30E-15
17 G860 AI486684 AI486684 EST245006 tomato ovary, TAMU Lycop... 3 224 5.60E-15
17 G860 BG415259 BG415259 HVSMEk0005M04f Hordeum vulgare tes... 2 223 5.70E-15
17 G860 AQ578837 AQ578837 nbxb0093F09f CUGI Rice BAC Library... -2 225 5.70E-15
17 G860 AW331118 AW331118 707047D10.x1 707 - Mixed adult tis... 3 224 5.70E-15
17 G860 BG318835 BG318835 NXPV_020_D12_F NXPV (Nsf Xylem Pla... 1 225 5.90E-15
17 G860 BE659918 BE659918 1299 GmaxSC Glycine max cDNA, mRNA... 2 224 6.10E-15
17 G860 BG631100 BG631100 cC-esflcLEL6H14a1 Tomato flower Ii... 1 226 6.10E-15
17 G860 BG343946 BG343946 HVSMEg0007D05f Hordeum vulgare pre... 3 223 6.10E-15
17 G860 BI925669 BI925669 EST545558 tomato flower, buds 0-3 ... 1 223 6.30E-15 c O 17 G860 AW277843 AW277843 sf87d01.y1 Gm-c1019 Glycine max cD... 1 224 6.40E-15
17 G860 ZMA292960 AJ292960 Zea mays mRNA for putative MADS-do... 1 235 6.40E-15
17 G860 BE195464 BE195464 HVSMEh0089L24f Hordeum vulgare 5-4... 2 223 6.50E-15
17 G860 AW697385 AW697385 NF116B04ST1 F1032 Developing stem M... 3 224 6.50E-15
17 G860 AW290406 AW290406 NXNV019H03F Nsf Xylem Normal wood ... 1 225 6.70E-15
17 G860 BI931105 BI931105 EST550994 tomato flower, 8 mm to p... 3 223 7.00E-15
17 G860 Bl 176052 BI176052 EST517135 cSTS Solanum tuberosum c... 1 223 7.10E-15
17 G860 BG599624 BG599624 EST504519 cSTS Solanum tuberosum c... 1 223 7.10E-15
17 G860 BG418254 BG418254 HVSMEk0022B16f Hordeum vulgare tes... 3 222 7.20E-15
17 G860 BI272847 BI272847 NF097G09FL1 F1071 Developing flower... 3 224 7.20E-15
17 G860 AF150934 AF150934 Physcomitrella patens MADS-domain ... 1 244 7.20E-15
17 G860 BF728292 BF728292 1000060C02.x1 1000 - Unigene I fro... 1 224 7.20E-15
17 G860 BF622888 BF622888 HVSMEa0009O05f Hordeum vulgare see... 2 223 7.30E-15
17 G860 AB050643 AB050643 Magnolia praecocissima mRNA for pu... 1 222 7.30E-15
17 G860 BM324751 BM324751 PIC1_35_B11.b1_A002 Pathogen-infec... 1 225 7.40E-15
17 G860 AI487729 AI487729 EST246051 tomato ovary, TAMU Lycop... 3 224 7.40E-15
17 G860 AI897047 AI897047 EST266490 tomato ovary, TAMU Lycop... 3 224 7.70E-15
17 G860 AF335244 AF335244 Petunia x hybrida MADS-box transcr... 1 222 7.70E-15
Table 2
17 G860 AI771416 AI771416 EST252516 tomato ovary, TAMU Lycop... 1 227 7.80E-15 1
17 G860 AB046596 AB046596 Cucumis sativus ERAF17 mRNA for pu... 3 222 7.80E-15 1
17 G860 AV933011 AV933011 AV933011 K. Sato unpublished cDNA ... 1 223 7.90E-15 1
17 G860 BE585982 BE585982 Est#7pT7_B09_b9_073 KSU wheat Fusa... 1 223 8.10E-15 1
17 G860 BM405355 BM405355 EST579682 potato roots Solanum tub... 1 223 8.10E-15 1
17 G860 BI958544 BI958544 HVSMEn0015l07f Hordeum vulgare rac... 3 223 8.10E-15 1
17 G860 BE499104 BE499104 WHE0960_C01_E02ZS Wheat pre-anthes... 3 223 8.10E-15 1
17 G860 AW623098 AW623098 EST321043 tomato flower buds 3-8 m... 1 224 8.20E-15 1
17 G860 BE432640 BE432640 EST399169 tomato breaker fruit, TI... 1 223 8.50E-15 1
17 G860 AI484624 AI484624 EST242885 tomato ovary, TAMU Lycop... 3 224 8.60E-15 1
17 G860 BG274189 BG274189 WHE2230_F01_K02ZS Aegilops speltoi... 2 224 8.60E-15 1
17 G860 AF029976 AF029976 Eucalyptus grandis MADS box protei... 2 224 8.60E-15 1
17 G860 AW219022 AW219022 EST301504 tomato root during/after... 3 223 8.70E-15 1
17 G860 AV934186 AV934186 AV934186 K. Sato unpublished cDNA ... 2 223 8.80E-15 1
17 G860 AV932867 AV932867 AV932867 K. Sato unpublished cDNA ... 2 223 8.90E-15 1
17 G860 BF657741 BF657741 OV2_17_D12.b1_A002 Ovary 2 (OV2) S... 3 223 8.90E-15 1
17 G860 LETDR3 X60756 L.esculentum TDR3 mRNA. 3/1992 3 223 8.90E-15 1 s 17 G860 BF429319 BF429319 WHE1804_B03_D06ZS Secale cereale a... 3 223 8.90E-15 1
° 17 G860 AV938919 AV938919 AV938919 K. Sato unpublished cDNA ... 3 223 9.10E-15 1
17 G860 BE606178 BE606178 WHE0913_H09_O17ZS Wheat 5-15 DAP s... 1 224 9.10E-15 1
17 G860 AB003322 AB003322 Oryza sativa mRNA for MADS box-lik... 3 189 9.20E-15 2
17 G860 BF317935 BF317935 OV1_9_F09.b1_A002 Ovary 1 (OV1) So... 3 223 9.20E-15 1
17 G860 AW039132 AW039132 EST281105 tomato mixed elicitor, B... 1 223 9.20E-15 1
17 G860 BE494811 BE494811 WHE1273_H12_P23ZS Secale cereale a... 1 223 9.20E-15 1
17 G860 BI272502 BI272502 NF023F04FL1 F1042 Developing flower... 1 224 9.30E-15 1
17 G860 C95289 C95289 C95289 Citrus unshiu Miyagawa-wase m... 1 224 9.30E-15 1
17 G860 BG837631 BG837631 Zm10_03a05_A Zm10_AAFC_ECORC_Fusar... -1 221 9.30E-15 1
17 G860 BM374839 BM374839 EBma05_SQ003_C14_R IGF Barley EBma... 1 223 9.30E-15 1
17 G860 GGN132208 AJ132208 Gnetum gnemon mRNA for putative MA... 3 228 9.40E-15 1
17 G860 AF254557 AF254557 Oryza sativa MADS (MADS) gene, com... 3 243 9.40E-15 1
17 G860 AP003379 AP003379 Oryza sativa (japonica cultivar-gr... 1 244 9.60E-15 1
17 G860 AF229199 AF229199 Oryza sativa chromosome 1 clone OS... 3 244 9.60E-15 1
17 G860 AI437956 AI437956 sa41 g 11.y1 Gm-d 004 Glycine max cD... 3 223 9.60E-15 1
17 G860 BE918857 BE918857 FM1_1_A12.b1_A003 Floral-Induced M... 1 223 9.60E-15 1
17 G860 BI928852 BI928852 EST548741 tomato flower, 3 - 8 mm ... 1 223 9.70E-15 1
17 G860 AW400298 AW400298 707057E09.X1 707 - Mixed adult tis... 1 222 9.80E-15 1
m rr rr rf rf rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rf rf rr rr rf rr rf rf rr rr rf
U OJ LOU LOU LOU LPU LOU UOJ LOU LOU LOU LOU LOU LOU LOU LOU LOU LOU LOU IOU LOU LOU O O O O O O O P P P O O O O O oi i-' ^ ^ ^ ^ ^ T^ ^ ^ T^ ^ ^ ^ T^ r-' ^ ^ ^ ^ ^ ^ ^ T^ ^ ^ ^ ^ ^ T-' r^ ^ T^ ^ ^ ^
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CM co m rf . w o ^ co C cn L m °2. CΛ ^ CM o g cO CD co m ^. — »>. |s- m co T- oo rs
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^ ^ S ^ c S cM c ^ fs. fc ^ cn o w mO rsM. O) T- cmo m tco; o-_o g°
» CΛ "r'T iffln SS ^o S Sϊ C S P S ^ > 5^O r ^n ^ ^M i W ^ (SOo l»,' lt c"O o n » 0i o'tr π'- co Ci- cnβ2t ^ oNj "θ lcoDj '!- co l' Pl-' 0r c)- CM c Cnm Oon OoOO' CM- Omϊ θ ι -2 LL § S LL § LU LL θ θ LL LU θ Lu 2 0 °2 LL m . LU θ < θ θ L ϊ α. θ θ £
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Figure imgf000192_0002
rs. rs. rs. rs. rs. rs. |s. rs. rs. r-- rs. rs. rs- |s* r. rs. rs. |s. rs. rs. r. rs. r. ^ Table 2
17 G860 AL380342 AL380342 MtBB51 H02F1 MtBB Medicago truncatu... 1 222 1.40E-14 1
17 G860 BG445265 BG445265 GA Ea0027H05f Gossypium arboreum ... 2 228 1.40E-14 1
17 G860 AF097747 AF097747 Cryptomeria japonica putative MADS... 1 220 1.50E-14 1
17 G860 BE434089 BE434089 EST405167 tomato breaker fruit, TI... 3 221 1.50E-14 1
17 G860 BG625803 BG625803 NXPV_066_C06_F NXPV (Nsf Xylem Pla... 2 222 1.50E-14 1
17 G860 AI771796 AI771796 EST252896 tomato ovary, TAMU Lycop... 3 221 1.50E-14 1
17 G860 AI486290 AI486290 EST244611 tomato ovary, TAMU Lycop... 3 221 1.60E-14 1
17 G860 BE433121 BE433121 EST399650 tomato breaker fruit, TI... 3 221 1.60E-14 1
17 G860 BG440326 BG440326 GA Ea0007H23f Gossypium arboreum ... 3 220 1.60E-14 1
17 G860 AI483740 AI483740 EST249611 tomato ovary, TAMU Lycop... 2 221 1.60E-14 1
17 G860 BI948755 BI948755 HVSMEI0010L12f Hordeum vulgare spi... 3 219 1.60E-14 1
17 G860 AW738740 AW738740 EST340167 tomato flower buds, anth... 1 220 1.60E-14 1
17 G860 AX027355 AX027355 Sequence 3 from Patent WO0037488. ... 2 231 1.70E-14 1
17 G860 BF479182 BF479182 L48-2837T3 Ice plant Lambda Uni-Za... 2 220 1.70E-14 1
17 G860 BF276751 BF276751 GA Eb0030l08f Gossypium arboreum ... 2 221 1.70E-14 1
17 G860 AW186417 AW186417 se67e07.y1 Gm-c1019 Glycine max cD... 3 220 1.70E-14 1
17 G860 AW129875 AW129875 707002A11.y1 707 - Mixed adult tis... 3 222 1.70E-14 1
S 17 G860 AI484747 AI484747 EST243008 tomato ovary, TAMU Lycop... 3 220 1.70E-14 1
M 17 G860 BG054385 BG054385 OV2_3_G06.b1_A002 Ovary 2 (OV2) So... 1 222 1.70E-14 1
17 G860 AW278878 AW278878 sf99h09.y1 Gm-c1019 Glycine max cD... 3 220 1.80E-14 1
17 G860 AW929667 AW929667 EST338455 tomato flower buds 8 mm ... 3 221 1.80E-14 1
17 G860 AI487178 AI487178 EST245500 tomato ovary, TAMU Lycop... 2 221 1.80E-14 1
17 G860 AI490605 AI490605 EST249159 tomato ovary, TAMU Lycop... 1 221 1.80E-14 1
17 G860 AI897069 AI897069 EST266512 tomato ovary, TAMU Lycop... 2 221 1.80E-14 1
17 G860 AW309115 AW309115 Sf93e09.y1 Gm-c1019 Glycine max cD... 3 220 1.80E-14 1
17 G860 AI483806 AI483806 EST249677 tomato ovary, TAMU Lycop... 1 221 1.80E-14 1
17 G860 BG131333 BG131333 EST464225 tomato crown gall Lycope... 3 221 1.90E-14 1
17 G860 BF050801 BF050801 EST435959 tomato developing/immatu... 2 220 1.90E-14 1
17 G860 BG789532 BG789532 sae65a02.y1 Gm-c1064 Glycine max c... 3 220 1.90E-14 1
17 G860 BI263356 BI263356 NF089C12PL1 F1098 Phosphate starved... 1 219 2.00E-14 1
17 G860 BG456966 BG456966 NF098D09PL1 F1076 Phosphate starved... 2 219 2.10E-14 1
17 G860 AW617743 AW617743 EST324154 L. hirsutum trichome, Co... 2 220 2.10E-14 1
17 G860 AF150931 AF150931 Physcomitrella patens MADS-domain ... 2 236 2.20E-14 1
17 G860 BE659970 BE659970 314 GmaxSC Glycine max cDNA, mRNA ... 2 220 2.20E-14 1
17 G860 BG417111 BG417111 HVSMEk0016F04f Hordeum vulgare tes... 3 220 2.20E-14 1
17 G860 LETDR8 X60760 L.esculentum TDR8 mRNA. 3/1992 3 218 2.30E-14 1
Table 2
G860 BE323629 BE323629 NF006D03PL1 F1026 Phosphate starved... 2 221 2.30E-14 1
G860 AF035439 AF035439 Cucumis sativus MADS box protein C... 3 231 2.30E-14 1
G860 BM137344 BM137344 WHE2632_F08_L16ZS Wheat Fusarium g... 1 219 2.40E-14 1
G860 ZMZMM3 Y09301 Z.mays mRNA for putative MADS-domain... 1 183 2.40E-14 2
G860 D89671 D89671 Ceratopteris richardii CerMADS2 mRNA... 1 236 2.60E-14 1
G860 OSU78890 U78890 Oryza sativa MADS box protein (OsMAD... 2 230 2.70E-14 1
G860 BNU22665 U22665 Brassica napus AGL15 type 1 gene, co... 3 238 2.70E-14 1
G860 BE330897 BE330897 so89f06.y1 Gm-c1041 Glycine max cD... 1 218 2.70E-14 1
G860 BE497689 BE497689 WHE955_D02_G03ZS Wheat pre-anthesi... 3 218 2.70E-14 1
G860 AY036062 AY036062 Brassica napus SHATTERPROOF1 (BnSH... 1 217 2.80E-14 1
G860 BF112397 BF112397 EST439987 tomato breaker fruit Lye... 3 221 2.90E-14 1
G860 GGN251557 AJ251557 Gnetum gnemon mRNA for putative MA... 2 235 2.90E-14 1
G860 BG 138098 BG138098 EST478540 wild tomato pollen Lycop... 2 218 3.00E-14 1
G860 BE346434 BE346434 sp25e06.y1 Gm-c1042 Glycine max cD... 1 218 3.00E-14 1
G860 BG 139571 BG139571 EST480109 wild tomato pollen Lycop... 2 218 3.00E-14 1
G860 AW745210 AW745210 LG1_387_D10.b1_A002 Light Grown 1 ... 3 218 3.00E-14 1
G860 BG357712 BG357712 OV2_29_D08.b1_A002 Ovary 2 (OV2) S... 3 218 3.10E-14 1
G860 BG596731 BG596731 EST495409 cSTS Solanum tuberosum c... 2 217 3.10E-14 1
G860 BI893993 BI893993 sai58e09.y1 Gm-c1068 Glycine max c... 3 218 3.10E-14 1
G860 AF150933 AF150933 Physcomitrella patens MADS-domain ... 2 236 3.20E-14 1
G860 BE659971 BE659971 363 GmaxSC Glycine max cDNA, mRNA ... 2 219 3.20E-14 1
G860 AF198176 AF198176 Dendrobium grex Madame Thong-IN MA... 3 217 3.20E-14 1
G860 AW928593 AW928593 EST337381 tomato flower buds 8 mm ... 2 221 3.20E-14 1
G860 BG357882 BG357882 OV2_31_D08.b1_A002 Ovary 2 (OV2) S... 3 218 3.20E-14 1
G860 BG649748 BG649748 RHIZ2_83_B06.b1_A003 Rhizome2 (RHI... 2 218 3.30E-14 1
G860 BF291862 BF291862 WHE2204_G11_N22ZS Aegilops speltoi... 2 218 3.30E-14 1
G860 BF291839 BF291839 WHE2204_E12_J24ZS Aegilops speltoi... 3 218 3.30E-14 1
G860 BG418810 BG418810 HVSMEk0024l17f Hordeum vulgare tes... 2 216 3.30E-14 1
G860 AI959798 AI959798 sc94d02.y1 Gm-c1019 Glycine max cD... 2 218 3.30E-14 1
G860 AF335238 AF335238 Petunia x hybrida MADS-box transcr... 1 216 3.40E-14 1
G860 BI405804 BI405804 146B05 Mature tuber lambda ZAP Sol... 1 217 3.40E-14 1
G860 BE495253 BE495253 WHE1269_B03_D05ZS Secale cereale a... 3 218 3.50E-14 1
G860 GGN132219 AJ132219 Gnetum gnemon mRNA for putative MA... 2 233 3.70E-14 1
G860 HVU249145 AJ249145 Hordeum vulgare mRNA for MADS-box ... 2 227 3.70E-14 1
G860 AW934066 AW934066 EST359909 tomato fruit mature gree... 3 218 3.80E-14 1
G860 BI543775 BI543775 FS6_F03 Sugar Beet inflorescence c... 2 216 3.90E-14 1
rf rf rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rr rf rf rf rr rr rr rr rr rr rr rr rr rr LU LU LU LU LU lXI LU ώ LU LU LU u!j Ll LU LU lIl LU LLI LU LU p o o o o o o o o σ o o o o o o o o o o o o o o o o σ o o o o o p p p p p r- -i-; M c n w m o i-s oq oq c» c» c»
*' ^ * ^ ^ ^ * * ^ * ^ * t * ^ ^ ^' ^ -i ιri ιιi ιd ιιi ifl io ι. ιo ιιi^ ιri ιιi iΛ io ιd ι ιi) oo cD i- i- m m i- p m rs. cD co P P P co ->- m oo cD P CD P P P m rr m m rr co p m cD i- m
OI CM CM C M CM CM CM o oJ OJ W CM CM CM CM CM CM J CM CM CM C CM CM CM CM CM C CM CM CM CM OI 'r- CM
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rs. r. r- |s. rs. r. rs. rs. r. r. r. r. rs- |s. rs. rs. r. rs. rs. rs. rs. ι. s. rs. rs. s. rs. r-^ CM
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Figure imgf000196_0002
co cD m m m cD m m m cD m co m m co m p co oo co m co cD m co p m m i- m m co m m m
C CM CM CM CM CM CM M CM CM CM CM CM CM CM OI CM CM CM CM CM OI OJ OJ OJ OJ OJ OJ -^ OJ OJ CM CM CM CM CM
W CM r- ^ i- CM '- CO CO CO CO CO CO r- CM CM N CO N N CO CM '- CO CO CO CM CJ CO C CO CO CM CO i- i-
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Figure imgf000196_0004
S g o l co r^ g S g S - ie w o ^ s S fe S ^ f r- g Si S g g S cM ^ *
Sδθ 5l§|§Sθ SSfe|§ θSur?mgLr?U -δΪ L5U -|*SθSS§§ ur-:UaJaL §gl" 3ssδ£8iLs slij L~LS-S2 cS-. -85 m m < < < m < m < co co co cQ < co < < CQ N < cQ co < < ϋ < < cQ < θQ < cQ ω < < < p p o o o o o o o o o o p o o o o o o o o o o o o o o o o o o o o o o o co co m co m m co m m m p m co m p m p m m m m m m m m m co o m m m co co co co cD co co co co co co co co co co co co co co co co co m co co co co co co co co co co co co co co co o co co O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O
rs. rs. r. rs. rs. rs. |s. r. rs. rs. r-. |s. | . ι . r-- r-- r-- rs. |s. rs. rs. r . rs. |^ Table 2
17 G860 PHFBP6A X68675 P.hybrida fbp6 mRNA. 8/2000 2 223 8.00E-14 1
17 G860 GHY9723 AJ009723 Gerbera hybrida mRNA for MADS-box ... 2 225 8.10E-14 1
17 G860 BE519003 BE519003946075F03.y1 946 - tassel primordi... 3 214 8.30E-14 1
17 G860 AI898236 AI898236 EST267679 tomato ovary, TAMU Lycop... 3 215 8.30E-14 1
17 G860 AP004741 AP004741 Oryza sativa chromosome 6 clone OS... 3 191 8.30E-14 2
17 G860 BG626444 BG626444 cC-esflcLEL12J22d1 Tomato flower I... 2 215 8.30E-14 1
17 G860 ZMU31522 U31522 Zea Mays MADS box protein (ZmOV23) m... 3 227 8.40E-14 1
17 G860 BM498342 BM498342 952022D12.X1 952 - BMS tissue from... 3 215 8.40E-14 1
17 G860 AI770966 AI770966 606063H05.x1 606 - Ear tissue cDNA... 1 214 8.40E-14 1
17 G860 AP004516 AP004516 Lotus japonicus genomic DNA, chrom... 3 235 8.40E-14 1
17 G860 BM428723 BM428723 952022D12.X3952 - BMS tissue from... 1 215 8.50E-14 1
17 G860 BE511439 BE511439 946060G10.y1 946 - tassel primordi... 1 214 8.60E-14 1
17 G860 AF029975 AF029975 Eucalyptus grandis MADS box protei... 1 226 8.70E-14 1
17 G860 AW981881 AW981881 PC19G08 Pine TriplEx pollen cone I... 1 214 8.80E-14 1
17 G860 BE511434 BE511434 946060F10.y1 946 - tassel primordi... 1 214 8.80E-14 1
17 G860 BE344090 BE344090 EST409252 potato stolon, Cornell U... 2 214 8.90E-14 1
17 G860 AF082531 AF082531 Pimpinella brachycarpa transcripti... 1 212 9.00E-14 1
17 G860 BE637039 BE637039 WHE1821 -1824J01J01ZS Secale cere... 2 215 9.00E-14 1
17 G860 AF335236 AF335236 Petunia x hybrida MADS-box transcr... 1 220 9.20E-14 1
17 G860 AI486250 AI486250 EST244571 tomato ovary, TAMU Lycop... 2 215 9.40E-14 1
17 G860 AI899370 AI899370 EST268813 tomato ovary, TAMU Lycop... 2 215 9.50E-14 1
17 G860 AM 61447 AI161447 A001 P09U Hybrid aspen plasmid libr... 3 216 9.50E-14 1
17 G860 CSCUS1 X97801 C.sativus CUS1 mRNA for put. transcr... 3 223 9.50E-14 1 π G860 BI957545 BI957545 HVSMEn0010B09f Hordeum vulgare rac... 3 213 9.60E-14 1
17 G860 CRCRM3 Y08239 C.richardii mRNA for CRM3 protein. 7... 2 218 9.60E-14 1
17 G860 AX006357 AX006357 Sequence 6 from Patent WO0004176. ... 2 228 9.60E-14 1
17 G860 AF035438 AF035438 Cucumis sativus MADS box protein C... 3 228 9.60E-14 1
17 G860 AF022379 AF022379 Cucumis sativus agamous-like putat... 1 228 9.70E-14 1
17 G860 AF265562 AF265562 Vitis vinifera MAD-box transcripio... 2 228 9.70E-14 1
17 G860 BM412293 BM412293 EST586620 tomato breaker fruit Lye... 1 212 1.00E-13 1
17 G860 BE921657 BE921657 EST425426 potato leaves and petiol... 2 213 1.10E-13 1
17 G860 AW277299 AW277299 sf80e05.y1 Gm-c1019 Glycine max cD... 3 213 1.10E-13 1
17 G860 AI486645 AI486645 EST244966 tomato ovary, TAMU Lycop... 2 212 1.10E-13 1
17 G860 BI928748 BI928748 EST548637 tomato flower, 3 - 8 mm ... 1 212 1.10E-13 1
17 G860 BE249689 BE249689 NF020D04LF1 F1041 Developing leaf M... 3 216 1.10E-13 1
17 G860 AI894541 AI894541 EST263984 tomato callus, TAMU Lyco... 1 215 1.20E-13 1
co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co co
LU LU LU LU IU LJJ LU LJJ LU LU LIJ LU I±J LU LU LU LU LU LU P P P O O O O O O P P O O O O O O O O O O O O O P P O O O O O O O O O O
01 CM CM CM CM CM C CM CM CM CM C ) CO C CO CO CO CO CO CO CO C CO C C rr rr rr rr rr rr rr rr rr rr rr
CM CM CO CM CM τ- CM O ι- CM CM CO CM '>- ι- P O ι- CO CM CM O τ- CM C m CM CM O CM O rr θJ OJ OJ CO t- τ- τ- τ- τ r τ- CO τ- τ- r- C τ- τ- r. C\I C τ- τ- τ- τ- C τ- τ- τ- C\l ^- ϊ- CO ϊ- C r τ- τ- CO CM CM CM CM OJ CM OI CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM OJ OJ OJ CM CM CM CM
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Figure imgf000198_0001
ι3 τ-
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P O P O P O O O O O O O O O O O O O O O O O O O O O O O O P O o o o rr rr m m m m m m m m m m m m r-- rs. i>. rs. rs. i-» oo cn o> o) 0) 0) 0) 0) CM CM m oό cό rr" c r-- m m o co cM C ^ oo m o m cM - ->- cD τ- c ι- co τ- m rr cn cM co oθ - τ- ^ rs. -^ σ)
CO CM ->- OJ OJ ι- OJ CO '- '- CM CM ι- ι- - ι- ->- CM t- τ- -^ τ- τ- CM OJ O OJ CM m m cθ rr rr CM CM - CΛI CM CM CM CM OI OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM
CM - CM 'r- CM ->- CO t- CO ι- - cO -r- CO CM ι- CM CO C CO '>- CM C - CM ι- - -^ ->- τ- - τ- --
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Figure imgf000199_0001
O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O CD CO P CO CD CO m CO CD CO CO CD CO CD CD CO CD P CO CO CO CD CO CD CO CD CO CO CO CO CO CO CD CD CD CO oo oo oo oo oo oo oo oo oo oo co oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo oo αo oo oo oo oo oo oo oo oo oooooooooooooooooooooooooooooooooooo r. ι. rs. rs. rs. rs. r-. rs. rs. r. r-- rs. r-- rs. |s. . rs. s. r-- rs. ι- r. rs. rs. r^ Table 2
G860 GI-4887235 AGAMOUS homolog transcription factor 1 234 4.40E-29 4
G860 GI-13810204 MADS1 protein [Cucumis sativus] 1 225 6.00E-29 4
G860 GI-431736 NAG1 1 225 2.50E-28 4
G860 GI-3913007 AG_TOBAC AGAMOUS PROTEIN (NAG1) 1 225 2.50E-28 4
G860 GI-7446523 T03592 floral homeotic protein NAG1 - comm... 1 225 2.50E-28 4
G860 GI-4837612 farinelli protein 1 218 6.70E-28 4
G860 GI-13384058 AF335240_1 MADS-box transcription factor F... 1 232 9.60E-28 4
G860 GI-1568513 fbp11 1 221 1.00E-27 4
G860 GI-457382 TAG1 1 221 2.10E-27 4
G860 GI-3913004 AG_LYCES AGAMOUS PROTEIN (TAG1) 1 221 2.10E-27 4
G860 GI-7446522 T07185 floral homeotic protein TAG1 - tomato 1 221 2.10E-27 4
G860 GI-16973296 C-type MADS box protein [Malus x domestica] 1 222 1.50E-26 3
G860 GI-264223 promotes sex organ development=ple [Antirr... 1 219 1.80E-26 4
G860 GI-322801 A44343 promotes sex organ development prot... 1 219 1.80E-26 4
G860 GI-2997615 CUM10 1 231 2.20E-26 3
G860 GI-4103342 agamous-like putative transcription factor 1 231 2.20E-26 3
G860 GI-7446521 T08040 MADS-box protein - cucumber 1 231 2.20E-26 3
G860 GI-396199 fbp6 1 223 3.70E-26 3
G860 GI-2129972 S60307 fbp6 protein - garden petunia 1 223 3.70E-26 3
G860 GI-4101710 MADS box transcription factor 1 230 4.60E-26 3
G860 GI-4103486 MADS box protein 1 230 5.80E-26 3
G860 GI-695688 DAL2 protein 1 230 5.80E-26 3
G860 GI-4033710 AGAMOUS-like MADS-box transcription factor... 1 230 5.80E-26 3
G860 GI-4033721 AGAMOUS-like MADS-box transcriptional fact... 1 230 5.80E-26 3
G860 GI-4033723 AGAMOUS-like MADS-box transcriptional fact... 1 230 5.80E-26 3
G860 GI-7446536 T14847 MADS-box protein dal2 - Norway spruce 1 230 5.80E-26 3
G860 GI-3646324 MADS-box protein 1 222 6.10E-26 3
G860 GI-5777904 U78949_1 MADS-box protein 3 1 222 6.10E-26 3
G860 GI-1206005 putative MADS-box family transcription factor 1 224 9.70E-26 3
G860 GI-1702951 MADS-box family transcription factor 1 224 9.70E-26 3
G860 GI-7446546 T10486 MADS box protein - Canadian red pine 1 224 9.70E-26 3
G860 GI-7446559 T09571 MADS box protein MADS2 - Monterey pine 1 224 9.70E-26 3
G860 GI-6006607 MADS box protein, MADS28 1 212 1.00E-25 3
G860 GI-4218160 MADS-box protein, GAGA1 1 225 1.20E-25 3
G860 GI-695686 dal1 1 221 1.20E-25 3
G860 GI-2160701 MADS-box protein 1 221 1.20E-25 3
Table 2
17 G860 GI-7446537 T14846 probable MADS-box protein dall - No... 1 221 1.20E-25 3
17 G860 GI-7446551 T09603 MADS-box protein 3 - Monterey pine 1 221 1.20E-25 3
17 G860 GI-4218162 MADS-box protein, GAGA2 1 225 1.50E-25 3
17 G860 GI-5031217 AGAMOUS homolog 1 227 1.90E-25 3
17 G860 GI-3646322 MADS-box protein 1 214 2.10E-25 3
17 G860 GI-6175371 AF091458_1 MADS box transcription factor M... 1 212 2.10E-25 3
17 G860 GI-16973298 C-type MADS box protein [Malus x domestica] 1 222 3.20E-25 3
17 G860 GI-167126 BAG1 1 226 5.00E-25 3
17 G860 GI-322650 A43484 probable transcription factor BAG1 ... 1 226 5.00E-25 3
17 G860 GI-399096 AG_BRANA AGAMOUS PROTEIN 1 226 5.00E-25 3
17 G860 GI-4103757 MADS1 1 224 5.10E-25 3
17 G860 GI-6092011 GpMADS4 1 223 5.10E-25 3
17 G860 GI-2981612 transcription factor 1 236 5.60E-25 2
17 G860 GI-6651033 AF129875_1 MADS box transcription factor M... 1 216 6.80E-25 3
17 G860 GI-3646340 MADS-box protein 1 230 7.40E-25 2
17 G860 GI-10188329 unnamed protein product [Zea mays] 1 231 9.30E-25 2
17 G860 GI-2997613 CUM1 1 228 1.00E-24 3 is, 17 G860 GI-4103346 agamous-like putative transcription factor 1 228 1.OOE-24 o 3
® 17 G860 GI-7446520 T08039 MADS-box protein - cucumber 1 228 1.OOE-24 3
17 G860 GI-9994504 FBP15 [Petunia x hybrida] 1 228 1.OOE-24 3
17 G860 GI-602900 SLM1 1 224 1.OOE-24 3
17 G860 G1-8745072 MADS box protein [Betula pendula] 1 224 1.OOE-24 3
17 G860 GI-12655901 AF226865_1 putative transcription factor A... 1 217 1.10E-24 3
17 G860 GI-17223670 SHATTERPROOF1 [Brassica napus] 1 217 1.10E-24 3
17 G860 GI-1067169 floral binding protein number 7 1 220 1.30E-24 3
17 G860 GI-13177638 AF265554_1 transcription factor CMB [Cucum... 1 225 1.70E-24 3
17 G860 GI-1483230 MADS4 protein 1 214 1.80E-24 3
17 G860 GI-1483228 MADS3 protein 1 203 1.90E-24 3
17 G860 GI-508577 box protein 1 231 2.40E-24 2
17 G860 GI-1076739 S53306 box protein - rice 1 231 2.40E-24 2
17 G860 GI-11493807 MADS box protein MADS1 [Oryza sativa] 1 231 2.40E-24 2
17 G860 GI-1914838 MADS box protein 1 230 2.50E-24 2
17 G860 GI-5295964 EST D15657(C1032) corresponds to a region... 1 230 2.50E-24 2
17 G860 GI-7446542 T04168 MADS box protein - rice 1 230 2.50E-24 2
17 G860 GI-2735764 MADS transcriptional factor; STMADS16 1 214 2.80E-24 3
17 G860 GI-7446547 T06995 probable MADS box transcription fac... 1 214 2.80E-24 3
Table 2
17 G860 GI-939779 MADS box protein 1 223 4.10E-24 2
17 G860 GI-7446514 T03398 MADS box protein - maize 1 223 4.10E-24 2
17 G860 GI-886405 MADS box protein 1 228 4.20E-24 3
17 G860 GI-2130078 S59480 MADS-box protein 3 - rice 1 228 4.20E-24 3
17 G860 GI-13161415 putative MADS-box protein [Oryza sativa] 1 228 4.20E-24 3
17 G860 GI-13958339 AF372840_1 madsl [Poa annua] 1 223 5.20E-24 2
17 G860 GI-313113 pMADS3 1 225 5.40E-24 3
17 G860 GI-478387 JQ2212 pMADS3 protein - garden petunia 1 225 5.40E-24 3
17 G860 GI-3913006 AG_PETHY AGAMOUS PROTEIN 1 225 5.40E-24 3
17 G860 GI-17827467 PMADS3 [Petunia x hybrida] 1 225 5.40E-24 3
17 G860 GI-3688591 MADS box transcription factor 1 223 6.60E-24 2
17 G860 GI-6650550 AF109153_1 MADS box transcription factor M... 1 223 6.60E-24 2
17 G860 GI-2286109 MADS box protein 1 223 8.40E-24 2
17 G860 GI-7446517 T04167 MADS box protein - rice 1 223 8.40E-24 2
17 G860 GI-7672991 AF144623_1 MADS-box transcription factor [... 1 212 9.50E-24 3
17 G860 GI-5019464 putative MADS domain transcription factor ... 1 233 1.00E-23 2
17 G860 GI-10188331 unnamed protein product [Zea mays] 1 231 1.00E-23 2
IN) 17 G860 GI-4033725 AGAMOUS-like MADS-box transcriptional fact... 1 208 1.20E-23 3
^ 17 G860 GI-9956938 AF150931_1 MADS-domain protein PPM1 [Physc... 1 236 1.30E-23 2
17 G860 GI-9956940 AF150932_1 MADS-domain protein PPM1 [Physc... 1 236 1.30E-23 2
17 G860 GI-9367311 MADS-box protein 7 [Hordeum vulgare] 1 227 1.70E-23 2
17 G860 GI-9964074 MADS-box transcription factor jointless [L... 1 223 1.70E-23 2
17 G860 GI-17433048 JOIN_LYCES MADS-box JOINTLESS protein (LeM... 1 223 1.70E-23 2
17 G860 GI-13384052 AF335237_1 MADS-box transcription factor F... 1 210 2.00E-23 3
17 G860 GI-18076209 putative MADS-domain transcription factor ... 1 236 2.10E-23 2
17 G860 GI-3688589 MADS box transcription factor 1 223 2.20E-23 2
17 G860 GI-16549058 putative MADS-domain transcription factor ... 1 222 2.20E-23 2
17 G860 GI-5019456 putative MADS domain transcription factor ... 1 221 2.20E-23 2
17 G860 GI-6092009 GpMADS3 1 221 2.20E-23 2
17 G860 GI-7544096 pMADS4 [Petunia x hybrida] 1 220 2.20E-23 2
17 G860 GI-15824795 AF345911_1 MADS-box protein FDRMADS3 [Oryz... 1 216 2.30E-23 2
17 G860 GI-5051933 MADS-box protein FDRMADS8 1 215 2.30E-23 2
17 G860 GI-18057092 AC092697_3 putative transcription factor [... 1 215 2.90E-23 2
17 G860 GI-6467974 AF198175_1 MADS box protein DOMADS2 1 210 2.90E-23 2
17 G860 GI-9956942 AF150933_1 MADS-domain protein PPM2 [Physc... 1 236 3.40E-23 2
17 G860 GI-9956944 AF150934_1 MADS-domain protein PPM2 [Physc... 1 236 3.40E-23 2
Table 2
17 G860 GI-9367313 MADS-box protein 8 [Hordeum vulgare] 1 216 3.60E-23 2
17 G860 GI-13384060 AF335241_1 MADS-box transcription factor F... 1 216 3.60E-23 2
17 G860 GI-5295986 MADS box-like protein 1 199 3.80E-23 4
17 G860 GI-6635740 AF207699_1 agamous-like MADS box protein O.. 1 215 3.90E-23 3
17 G860 GI-14279306 AF265562_1 MAD-box transcripion factor [Vi... 1 228 4.40E-23 2
17 G860 GI-5019429 putative MADS domain transcription factor ... 1 228 4.40E-23 2
17 G860 GI-4204232 MADS-box protein 1 1 220 4.60E-23 2
17 G860 GI-939781 MADS box protein 1 219 4.60E-23 2
17 G860 GI-7446515 T03408 MADS box protein - maize 1 219 4.60E-23 2
17 G860 GI-5616513 AF168468_1 agamous protein 1 225 4.60E-23 3
17 G860 GI-3493647 transcription activator 1 212 5.00E-23 3
17 G860 GI-3646326 MADS-box protein 1 229 5.60E-23 2
17 G860 GI-5019460 putative MADS domain transcription factor ... 1 223 5.80E-23 2
17 G860 GI-4322475 putative MADS box transcription factor ETL 1 222 5.80E-23 2
17 G860 GI-951172 MADS box protein 1 228 7.30E-23 3
17 G860 GI-1001934 ZAG2 1 228 7.30E-23 3
17 G860 GI-12002141 AF112150_1 MADS box protein 3 [Zea mays] 1 219 7.40E-23 2
Is)
© 17 G860 GI-8574457 AF072534_1 pepper MADS-box protein [Capsie. 1 225 9.20E-23 2 Is) 17 G860 GI-5777906 U78950_1 MADS-box protein 4 1 219 9.40E-23 2
17 G860 GI-4204234 MADS-box protein 2 1 215 9.60E-23 2
17 G860 GI-1001935 ZMM1 1 227 1.20E-22 3
17 G860 GI-1167914 MADS box protein 1 227 1.20E-22 3
17 G860 GI-7446525 T02261 MADS box protein - maize 1 227 1.20E-22 3
17 G860 GI-848999 agamous protein 1 223 1.20E-22 2
17 G860 GI-6970411 MADS-box protein 1 222 1.20E-22 2
17 G860 GI-13384066 AF335244_1 MADS-box transcription factor F... 1 222 1.20E-22 2
17 G860 GI-13384050 AF335236_1 MADS-box transcription factor F... 1 220 1.20E-22 2
17 G860 GI-2981614 transcription factor 1 217 1.20E-22 3
17 G860 GI-3114584 MADS box protein 1 226 1.50E-22 2
17 G860 GI-13384046 AF335234_1 MADS-box transcription factor F... 1 222 1.50E-22 2
17 G860 GI-6468286 putative MADS domain transcription factor ... 1 221 1.50E-22 2
17 G860 GI-6970415 MADS-box protein 1 225 1.50E-22 3
17 G860 GI-3290209 MADS-box protein 1 1 220 1.50E-22 2
17 G860 GI-3646334 MdMADS8 1 220 1.50E-22 2
17 G860 GI-5070138 AF068722_1 MADS-box protein MADS3 1 220 1.50E-22 2
17 G860 GI-7488622 T17023 MADS box protein 1 - apple tree 1 220 1.50E-22 2
Table 2
17 G860 GI-7592642 API-like MADS box protein [Oryza sativa] 212 1.60E-22 2
17 G860 GI-320596 JQ1690 floral binding protein 2 - garden p... 220 1.90E-22 2
17 G860 GI-1181186 transcription factor 220 1.90E-22 2
17 G860 GI-1345965 FBP2_PETHY FLORAL HOMEOTIC PROTEIN FBP2 (F... 220 1.90E-22 2
17 G860 GI-18650789 AF234617_1 MADS-box transcription factor [... 226 2.40E-22 3
17 G860 GI-4096982 AGAMOUS protein 225 2.40E-22 3
17 G860 GI-6970413 MADS-box protein 225 2.40E-22 3
17 G860 GI-6970417 MADS-box protein 225 2.40E-22 3
17 G860 GI-861081 agamous 222 2.40E-22 2
17 G860 GI-3913005 AG_PANGI AGAMOUS PROTEIN (GAG2) 222 2.40E-22 2
17 G860 GI-1206003 putative MADS-box family transcription factor 222 2.40E-22 2
17 G860 GI-7446558 T09569 MADS box protein MADS1 - Monterey pine 222 2.40E-22 2
17 G860 GI-1049022 transcription factor SaMADS A 220 2.50E-22 2
17 G860 GI-7446552 T10422 MADS box protein A - white mustard 220 2.50E-22 2
17 G860 GI-1239961 MADS-box gene 219 2.50E-22 2
17 G860 GI-7446512 S78015 MADS box protein DEFH49 - garden sn... 219 2.50E-22 2
17 G860 GI-6652756 MADS box protein 217 2.50E-22 2
Is) 17 G860 GI-13448658 MADS box transcription factor [Ipomoea bat... 214 2.50E-22 2
© Ui 17 G860 GI-12019658 FDRMADS6 [Oryza sativa] 210 2.60E-22 2
17 G860 GI-6634708 AF176782_1 MADS box transcription factor 208 2.60E-22 2
17 G860 GI-6731756 unnamed protein product 208 2.60E-22 2
17 G860 GI-11037010 AF305696_1 MADS-box protein EAP2S [Eucalyp... 196 2.70E-22 2
17 G860 GI-11120557 AF306349_1 MADS box protein AP2L [Eucalypt... 196 2.70E-22 2
17 G860 GI-13384056 AF335239_1 MADS-box transcription factor F... 222 3.10E-22 2
17 G860 GI-1617211 MADS D 219 3.10E-22 2
17 G860 G1-3912986 AGL9_SINAL FLORAL HOMEOTIC PROTEIN AGL9 HO... 219 3.10E-22 2
17 G860 GI-7446553 T10467 MADS box protein D - white mustard 219 3.10E-22 2
17 G860 GI-3170502 APETALA3 homolog PnAP3-2 218 3.20E-22 2
17 G860 GI-939785 MADS box protein 216 3.20E-22 2
17 G860 GI-7446509 T03410 MADS box protein - maize 216 3.20E-22 2
17 G860 GI-18996775 AF462152_1 MADS-box transcription factor H... 212 3.20E-22 2
17 G860 GI-10880315 putative MADS-domain transcription factor ... 235 3.70E-22 2
17 G860 GI-887579 MADS box regulatory protein 224 3.90E-22 2
17 G860 GI-1046276 MADS box regulatory protein 224 3.90E-22 2
17 G860 GI-1364102 S57586 MADS box regulatory protein - Rumex... 224 3.90E-22 2
17 G860 GI-1321797 putative transcription factor 223 3.90E-22 2
Table 2
17 G860 GI-4103344 agamous-like putative transcription factor 1 223 3.90E-22 2
17 G860 GI-7446562 T10185 MADS-box protein CUS1 - cucumber 1 223 3.90E-22 2
17 G860 GI-13384048 AF335235_1 MADS-box transcription factor F... 1 214 4.10E-22 2
17 G860 GI-5295984 MADS box-like protein 1 212 4.10E-22 2
17 G860 GI-9367315 MADS-box protein 9 [Hordeum vulgare] 1 216 5.10E-22 2
17 G860 GI-17432174 MADS-box protein [Lycopersicon esculentum] 1 215 5.20E-22 2
17 G860 GI-695317 MADS box protein 1 215 5.20E-22 2
17 G860 GI-3023536 CMB1_DIACA MADS BOX PROTEIN CMB1 1 215 5.20E-22 2
17 G860 GI-7446563 T10714 MADS-box protein CMB1 - clove pink 1 215 5.20E-22 2
17 G860 GI-1905930 MADS box protein 1 217 6.50E-22 2
17 G860 GI-7446519 T04335 MADS box protein - rice 1 217 6.50E-22 2
17 G860 GI-1905944 MADS box transcription factor SbMADSI 1 216 6.50E-22 2
17 G860 GI-7446545 T14801 MADS box protein MADS1 - sorghum 1 216 6.50E-22 2
17 G860 GI-14518447 AF130118_1 MADS box protein [Capsicum annuum] 1 212 6.60E-22 2
17 G860 GI-6468290 putative MADS domain transcription factor ... 1 211 6.70E-22 2
17 G860 GI-5295982 MADS box-like protein 1 226 8.00E-22 2
17 G860 GI-6467976 AF198176_1 MADS box protein DOMADS3 1 217 8.30E-22 2
Is) 17 G860 GI-13448660 MADS box transcription factor [Ipomoea bat... 1 212 8.50E-22 2
© 4- 17 G860 GI-5019431 putative MADS domain transcription factor ... 1 231 1.00E-21 2
17 G860 GI-2529340 homologue; putative 1 222 1.00E-21 3
17 G860 GI-7446524 T01700 hypothetical protein - maize 1 222 1.00E-21 3
17 G860 GI-431908 transcription factor 1 222 1.00E-21 2
17 G860 GI-1076646 S46526 transcription factor - common tobacco 1 222 1.00E-21 2
17 G860 GI-3688593 MADS box transcription factor 1 218 1.00E-21 2
17 G860 GI-2286113 MADS box protein 1 216 1.10E-21 2
17 G860 GI-7446544 T04170 MADS box protein - rice 1 216 1.10E-21 2
17 G860 GI-19358 TDR5 1 214 1.10E-21 2
17 G860 GI-19384 TDR5 1 214 1.10E-21 2
17 G860 GI-629674 S23728 TDR5 protein - tomato 1 214 1.10E-21 2
17 G860 GI-3913002 AGL9J.YCES FLORAL HOMEOTIC PROTEIN AGL9 HO... 1 214 1.10E-21 2
17 G860 GI-19382 TDR4 1 209 1.10E-21 2
17 G860 GI-542035 S23730 TDR4 protein - tomato 1 209 1.10E-21 2
17 G860 GI-3912999 AGL8J.YCES FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 209 1.10E-21 2
17 G860 GI-3947985 MADS-box protein 2 1 205 1.10E-21 2
17 G860 GI-6606070 AF058697_1 MADS14 protein 1 204 1.10E-21 2
17 G860 GI-1239959 MADS-box gene 1 221 1.30E-21 2
Table 2
17 G860 GI-7446511 S71757 MADS box protein DEFH200 - garden s... 1 221 1.30E-21 2
17 G860 GI-2463333 M79 protein 1 220 1.30E-21 2
17 G860 GI-7446518 T04307 M79 protein - rice 1 220 1.30E-21 2
17 G860 GI-16052 SQUA 1 210 1.40E-21 2
17 G860 GI-82313 S20886 transcription factor squa - garden ... 1 210 1.40E-21 2
17 G860 GI-9367307 MADS-box protein 3 [Hordeum vulgare] 1 207 1.40E-21 2
17 G860 GI-10946429 AF305076_1 MADS-box protein EAP1 [Eucalypt... 1 201 1.40E-21 2
17 G860 GI-15077026 AF286649_1 transcription factor CMB1 [Cucu... 1 198 1.50E-21 3
17 G860 GI-6840998 AF120097_1 DEF/GLO-like protein 1 222 1.70E-21 2
17 G860 GI-13384062 AF335242_1 MADS-box transcription factor F... 1 221 1.70E-21 2
17 G860 GI-6683777 AF135962_1 CAGL2 1 217 1.70E-21 2
17 G860 GI-4406132 MADS box protein 1 216 1.70E-21 2
17 G860 GI-5295980 MADS box-like protein 1 216 1.70E-21 2
17 G860 GI-16417764 AF424549_1 MADS-box protein SPW1 [Oryza sa... 1 216 1.70E-21 2
17 G860 GI-3184054 MADS-box transcription factor 1 215 1.70E-21 2
17 G860 GI-7446516 T06543 MADS box protein - garden pea 1 215 1.70E-21 2
17 G860 GI-16549085 putative MADS-domain transcription factor ... 1 210 1.70E-21 2 17 G860 GI-6606306 AF176783_1 floral binding protein 26 1 209 1.80E-21 2 17 G860 GI-1049024 transcription factor SaMADS B 1 209 1.80E-21 2
17 G860 GI-3913000 AGL8_SINAL FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 209 1.80E-21 2
17 G860 GI-1483232 MADS5 protein 1 206 1.80E-21 2
17 G860 GI-642591 amino acid feature: K-box, bp 283..480; am... 1 204 1.80E-21 2
17 G860 GI-642593 amino acid feature: MADS box; codes for a ... 1 204 1.80E-21 2
17 G860 GI-1561784 homeotic protein boiCAL 1 204 1.80E-21 2
17 G860 GI-6469345 DNA-binding protein 1 204 1.80E-21 2 7 G860 GI-7446539 T14456 MADS box protein homolog CAL - wild... 1 204 1.80E-21 2 7 G860 GI-7488599 T14457 MADS box protein homolog CAL - broc... 1 204 1.80E-21 2 7 G860 GI-12597207 putative MADS-box protein [Cucumis sativus] 1 222 2.10E-21 2 7 G860 GI-15808691 AF185574_1 transcription factor MAGL4 [Pop... 1 213 2.20E-21 2
17 G860 GI-6606072 AF058698_1 MADS15 protein 1 212 2.20E-21 2
17 G860 GI-4218175 MADS-box protein, GSQUA1 1 209 2.20E-21 2
17 G860 GI-758565 transcription factor 1 209 2.20E-21 2
17 G860 GI-758567 transcription factor 1 209 2.20E-21 2
17 G860 GI-3913001 AGL8_SOLTU FLORAL HOMEOTIC PROTEIN AGL8 HO... 1 209 2.20E-21 2
17 G860 GI-7446508 T07100 MADS box protein homolog POTM1-1 - ... 1 209 2.20E-21 2
17 G860 GI-2507629 putative MADS box transcription factor PrM... 1 207 2.20E-21 2
Table 2
17 G860 GI-3337237 putative MADS box transcription factor PrM... 1 207 2.20E-21 2
17 G860 GI-7446556 T10776 probable MADS box protein MADS6 - M... 1 207 2.20E-21 2
17 G860 GI-7446557 T10778 probable MADS' box protein MADS8 - M... 1 207 2.20E-21 2
17 G860 GI-5070142 AF068725_1 MADS-box protein MADS1 1 206 2.30E-21 2
17 G860 GI-602908 SLM5 1 205 2.30E-21 2
17 G860 GI-9367309 MADS-box protein 5 [Hordeum vulgare] 1 204 2.30E-21 2
17 G860 GI-169254 transcription factor 1 229 2.60E-21 2
17 G860 GI-320595 JQ1689 floral binding protein 1 - garden p... 1 229 2.60E-21 2
17 G860 GI-729464 FBP1_PETHY FLORAL HOMEOTIC PROTEIN FBP1 (F... 1 229 2.60E-21 2
17 G860 GI-2293892 unnamed protein product 1 229 2.60E-21 2
17 G860 GI-11494137 AF209729_1 putative MADS box transcription... 1 214 2.80E-21 2
17 G860 GI-5295990 MADS box-like protein 1 214 2.80E-21 2
17 G860 GI-16549070 putative MADS-domain transcription factor ... 1 214 2.80E-21 2
17 G860 GI-4102111 NAP1-1 1 212 2.80E-21 2
17 G860 GI-695690 DAL3 protein 1 212 2.80E-21 2
17 G860 GI-7484434 T14848 MADS-box protein dal3 - Norway spruce 1 212 2.80E-21 2
17 G860 GI-2507623 putative MADS box transcription factor PrM... 1 222 3.40E-21 2
Is) 17 G860 GI-7446554 T10751 MADS-box protein MADS9 - Monterey pine 1 222 3.40E-21 2
© βs 17 G860 GI-309574 homologue of Arabidopsis gene AGAMOUS 1 215 3.50E-21 3
17 G860 GI-542191 JQ2289 floral homeotic protein ZAG1 - maize 1 215 3.50E-21 3
17 G860 GI-13384068 AF335245_1 MADS-box transcription factor F... 1 211 3.60E-21 2
17 G860 GI-2507625 putative MADS box transcription factor PrM... 1 203 3.70E-21 2
1 G860 GI-7446564 T10764 probable MADS box protein MADS4 - M... 1 203 3.70E-21 2
17 G860 GI-10803404 MADS box protein [Gerbera hybrida] 1 217 4.50E-21 2
17 G860 GI-1239963 MADS-box gene 1 216 4.50E-21 2
17 G860 GI-7446510 S71756 MADS box protein DEFH72 - garden sn... 1 216 4.50E-21 2
17 G860 GI-6580949 AF158544_1 MADS-box transcription factor 1 212 4.50E-21 2
17 G860 GI-8745070 MADS box protein [Betula pendula] 1 211 4.60E-21 2
17 G860 GI-2290778 MADS box transcription factor 1 209 4.60E-21 2
17 G860 GI-3912987 AGL8_SOLCO FLORAL HOMEOTIC PROTEIN AGL8 HO. 1 209 4.60E-21 2
17 G860 GI-7446507 T07902 MADS box protein - Commerson's wild... 1 209 4.60E-21 2
17 G860 GI-16874557 MADS-box transcription factor DEFH28 [Anti... 1 209 4.60E-21 2
17 G860 GI-13274178 putative MADS-domain transcription factor ... 1 247 5.00E-21 2
17 G860 GI-19380 TDR3 1 223 5.50E-21 2
17 G860 GI-542034 S23729 TDR3 protein - tomato 1 223 5.50E-21 2
17 G860 GI-2507627 putative MADS box transcription factor PrM... 1 219 5.60E-21 2
Table 2
17 G860 GI-7446555 T10767 probable MADS box protein MADS5 - M... 1 219 5.60E-21 2
17 G860 GI-13384054 AF335238_1 MADS-box transcription factor F... 1 216 5.70E-21 2
17 G860 GI-6580947 AF158543_1 MADS-box transcription factor 1 212 5.80E-21 2
17 G860 GI-8567991 AF068723_1 MADS-box protein MADS4 [Nicotia... 1 211 5.80E-21 2
17 G860 GI-4218173 MADS-box protein, GGL01 1 209 5.80E-21 3
17 G860 GI-7328575 AF181479_1 MADS-box DNA binding protein [Z... 1 216 7.20E-21 2
17 G860 GI-5070144 AF068726_1 MADS-box protein MADS2 1 200 7.70E-21 2
17 G860 GI-12002139 AF112148_1 MADS box protein 1 [Zea mays] 1 212 9.30E-21 2
17 G860 GI-12964064 MADS box transcription factor [Oryza sativa] 1 205 9.60E-21 2
17 G860 GI-3646320 MADS-box protein 1 199 9.80E-21 2
17 G860 GI-13442962 AF095646_1 MADS box protein nmads3 [Oryza ... 1 193 1.00E-20 2
17 G860 GI-5295978 MADS box-like protein 1 189 1.00E-20 2
17 G860 GI-3114588 MADS box protein 1 222 1.10E-20 2
17 G860 GI-5230654 MADS-box protein 1 175 1.20E-20 4
17 G860 GI-4102113 NAP1-2 1 201 1.20E-20 2
17 G860 GI-16549062 putative MADS-domain transcription factor ... 1 180 1.40E-20 3
17 G860 GI-3646336 MdMADS9 1 196 1.60E-20 2
Is) 17 G860 GI-3986689 MADS box protein 1 210 1.90E-20 2 © -4 17 G860 GI-9367232 MADS box protein 1 [Hordeum vulgare] 1 203 2.00E-20 2
17 G860 GI-9367234 MADS-box protein 1 -2 [Hordeum vulgare] 1 203 2.00E-20 2
17 G860 GI-5070140 AF068724_1 MADS-box protein MADS5 1 199 2.00E-20 2
17 G860 GI-19871 NTGLOBOSA 1 229 2.30E-20 2
17 G860 GI-417063 GLOB_TOBAC FLORAL HOMEOTIC PROTEIN GLOBOSA 1 229 2.30E-20 2
17 G860 GI-448288 1916408A NTGLO gene [Nicotiana tabacum] 1 229 2.30E-20 2
17 G860 GI-486750 S35226 homeotic protein globosa homolog - ... 1 229 2.30E-20 2
17 G860 GI-6580945 AF158542_1 MADS-box transcription factor 1 210 2.50E-20 2
17 G860 GI-6580943 AF158541_1 MADS-box transcription factor 1 210 2.50E-20 2
17 G860 GI-1870206 MADS-box protein 1 208 3.10E-20 2
17 G860 GI-609253 Saapl 1 200 3.20E-20 2
17 G860 GI-1076477 S52236 Saapl protein - white mustard 1 200 3.20E-20 2
17 G860 GI-3913047 AP1_SINAL FLORAL HOMEOTIC PROTEIN APETALA1... 1 200 3.20E-20 2
17 G860 GI-1561780 homeotic protein boi2AP1 1 198 3.30E-20 2
17 G860 GI-4416347 MADS C-2 protein; MADS-box protein 1 198 3.30E-20 2
17 G860 GI-1905934 MADS box protein 1 194 3.30E-20 2
17 G860 GI-7489647 T14737 MADS box protein - sorghum (fragment) 1 194 3.30E-20 2
17 G860 GI-13274182 putative MADS-domain transcription factor ... 1 239 3.50E-20 2
Table 2
17 G860 GI-4105097 MADS box protein 26 1 215 3.90E-20 2
17 G860 GI-4433623 MADS-box transcription factor 1 215 3.90E-20 2
17 G860 GI-6467972 AF198174_1 MADS box protein DOMADS1 1 215 3.90E-20 2
17 G860 GI-2827300 MADS-box protein NMH 7 1 209 4.00E-20 2
17 G860 GI-7446532 T09335 MADS-box protein NMH 7 - alfalfa 1 209 4.00E-20 2
17 G860 GI-887392 BOAP1 1 196 5.30E-20 2
17 G860 GI-22665 GP (green petal) 1 211 6.40E-20 2
17 G860 GI-322773 S31693 transcription factor gp - garden pe... 1 211 6.40E-20 2
17 G860 GI-729974 MAD1_PETHY FLORAL HOMEOTIC PROTEIN PMADS1 ... 1 211 6.40E-20 2
17 G860 GI-1370276 MADS-box protein 1 211 6.40E-20 2
17 G860 GI-602906 SLM4 1 202 6.40E-20 3
17 G860 GI-13446154 MADS-box transcription factor [Pisum sativum] 1 198 6.70E-20 2
17 G860 GI-13661024 MADS-box transcription factor [Pisum sativum] 1 198 6.70E-20 2
17 G860 GI-18252655 AF461740_1 MADS-box transcription factor M... 1 198 6.70E-20 2
17 G860 GI-1561778 homeotic protein boi1AP1 1 196 6.80E-20 2
17 G860 GI-6580939 AF158539_1 MADS-box transcription factor 1 203 8.10E-20 3
17 G860 GI-6580941 AF158540_1 MADS-box transcription factor 1 203 8.10E-20 3
17 G860 GI-6092007 GpMADSI 1 183 9.00E-20 3
17 G860 GI-6470126 AF151693_1 transcription factor 1 231 9.60E-20 2
17 G860 GI-8216957 putative transcription factor [Cucumis sat... 1 206 1.10E-19 2
17 G860 GI-439239 MADS-box protein 1 200 1.10E-19 2
17 G860 GI-632112 S40405 MADS-box protein - Aranda deborah 1 200 1.10E-19 2
17 G860 GI-3912996 AGL9_ARADE FLORAL HOMEOTIC PROTEIN AGL9 HO... 1 200 1.10E-19 2
17 G860 GI-2293894 unnamed protein product 1 194 1.10E-19 2
17 G860 GI-16549060 putative MADS-domain transcription factor ... 1 168 1.20E-19 3
17 G860 GI-511065 deficiens analogue 1 208 1.30E-19 2 7 G860 GI-7446533 T07410 MADS box protein homolog DEF2 - potato 1 208 1.30E-19 2 7 G860 GI-3851331 putative MADS-domain transcription factor 1 183 1.50E-19 2
17 G860 GI-431226 deficiens analogue 1 207 1.70E-19 2
17 G860 GI-7446549 T07066 MADS-box protein homolog DEF4 - potato 1 207 1.70E-19 2
17 G860 GI-3892652 putative MADS-domain transcription factor 1 181 1.90E-19 2
17 G860 GI-16024 globosa 1 221 2.00E-19 2
17 G860 GI-282981 S28062 homeotic protein globosa - garden s... 1 221 2.00E-19 2
17 G860 GI-417062 GLOB-ANTMA FLORAL HOMEOTIC PROTEIN GLOBOSA 1 221 2.00E-19 2
17 G860 GI-3170464 APETALA3 homolog PnPI-1 1 224 2.60E-19 2
17 G860 GI-2735766 MADS transcriptional factor; STMADS11 1 207 2.70E-19 2
Table 2
G860 GI-7446550 T06996 MADS-box transcription factor MADS1... 1 207 2.70E-19 2
G860 GI-5051937 MADS-box protein FDRMADS2 1 182 3.00E-19 2
G860 GI-3114586 MADS box protein 1 224 3.30E-19 2
G860 GI-4885034 AF134114_1 PISTILLATA protein homolog HPH 1 215 3.40E-19 2
G860 GI-886401 MADS box protein 1 243 3.70E-19 1
G860 GI-7446534 T03894 MADS box protein - rice 1 243 3.70E-19 1
G860 GI-13272279 MADS [Oryza sativa] 1 243 3.70E-19 1
G860 GI-3253149 CMADS2 1 208 5.60E-19 2
G860 GI-17933456 MADS-box protein [Brassica napus] 1 202 5.70E-19 2
G860 GI-790637 AGL15 1 227 6.60E-19 2
G860 GI-3831486 AG15_BRANA FLORAL HOMEOTIC PROTEIN AGL15 1 227 6.60E-19 2
G860 GI-7446560 T07867 MADS box protein AGL15 (type 1) - rape 1 227 6.60E-19 2
G860 GI-8163950 AF230704_1 MADS box transcription factor T... 1 204 7.20E-19 2
G860 GI-10835358 PTD protein [Populus balsamifera subsp. tr... 1 199 7.40E-19 2
G860 GI-7677036 FDRMADS7 [Oryza sativa] 1 171 8.10E-19 3
G860 GI-16549083 putative MADS-domain transcription factor ... 1 172 8.10E-19 2
G860 GI-2961437 MADS box protein 1 226 8.50E-19 2
G860 GI-7446535 T03902 MADS4 box protein - rice 1 226 8.50E-19 2
G860 GI-602902 SLM2 1 203 9.20E-19 2
G860 GI-16973294 B-type MADS box protein [Malus x domestica] 1 192 9.60E-19 2
G860 GI-12666535 Pistillata MADS-box protein [Malus x domes... 1 211 1.10E-18 2
G860 GI-13384064 AF335243_1 MADS-box transcription factor F... 1 196 1.20E-18 2
G860 GI-19388 TDR8 1 218 1.40E-18 2
G860 GI-542036 S23732 TDR8 protein - tomato 1 218 1.40E-18 2
G860 GI-15667640 AF097747_1 putative MADS-box family transc... 1 220 1.80E-18 2
G860 GI-1561782 homeotic protein boil AP3 1 204 1.90E-18 2
G860 GI-6841082 AF124814_1 APETALA3 1 204 1.90E-18 2
G860 GI-5051935 MADS-box protein FDRMADS1 1 184 2.00E-18 2
G860 GI-12666533 Pistillata MADS-box protein [Malus x domes... 1 211 2.30E-18 2
G860 GI-4218169 MADs-box protein, GDEF1 1 205 2.40E-18 2
G860 GI-10880311 putative MADS-domain transcription factor ... 1 201 2.40E-18 2
G860 GI-16018 DEF A protein 1 199 2.40E-18 2
G860 GI-16020 deficiens 1 199 2.40E-18 2
G860 GI-100479 S12378 defA-1 protein - garden snapdragon 1 199 2.40E-18 2
G860 GI-100483 S19232 gene deficiens protein - garden sna... 1 199 2.40E-18 2
G860 GI-118426 DEFA ANTMA FLORAL HOMEOTIC PROTEIN DEFICIENS 1 199 2.40E-18 2
Table 2
17 G860 GI-3253147 CMADS1 1 227 2.50E-18 3
17 G860 GI-13274180 putative MADS-domain transcription factor ... 1 235 2.60E-18 1
17 G860 GI-2981610 transcription factor 1 199 4.00E-18 2
17 G860 GI-15216293 MADS-box protein [Rosa rugosa] 1 192 4.10E-18 2
17 G860 GI-8163952 AF230705_1 MADS box transcription factor A... 1 187 4.10E-18 2
17 G860 GI-3170512 APETALA3 homolog PhAP3 1 181 4.20E-18 2
17 G860 GI-22667 PMADS2 1 211 4.80E-18 2
17 G860 GI-322775 S31707 transcription factor pmads2 - garde... 1 211 4.80E-18 2
17 G860 GI-729976 MAD2_PETHY FLORAL HOMEOTIC PROTEIN PMADS 2 1 211 4.80E-18 2
17 G860 GI-16549081 putative MADS-domain transcription factor ... 1 178 6.90E-18 2
17 G860 GI-1561786 homeotic protein boι'2AP3 1 204 1.00E-17 2
17 G860 GI-7446540 T14473 MADS box protein 2AP3 - broccoli 1 204 1.00E-17 2
17 G860 GI-454265 FBP3 1 211 1.30E-17 2
17 G860 GI-2129971 S60288 FBP3 protein - garden petunia 1 211 1.30E-17 2
17 G860 GI-1362199 555773 homeotic protein ZAG2 - maize (frag... 1 228 1.50E-17 1
17 G860 GI-887575 MADS box regulatory protein 1 202 1.60E-17 2
17 G860 GI-1046272 MADS box regulatory protein 1 202 1.60E-17 2 17 G860 GI-1362200 555774 homeotic protein ZMM1 - maize (frag... 1 227 1.90E-17 1 17 G860 GI-8163966 AF230712_1 MADS box containing protein PI ... 1 207 2.10E-17 2
17 G860 GI-15667638 AF097746_1 putative MADS-box family transc... 1 226 2.40E-17 1
17 G860 GI-2055376 MADS box protein 1 174 2.90E-17 2
17 G860 GI-10880313 putative MADS-domain transcription factor ... 1 222 6.50E-17 1
17 G860 GI-9857312 MADS-box protein [Rosa rugosa] 1 203 6.90E-17 2
17 G860 GI-1006768 ZMM2 1 176 7.00E-17 3
17 G860 GI-7446528 T04388 AGAMOUS-like MADS-box protein ZMM2 ... 1 176 7.00E-17 3 7 G860 GI-1621333 homeotic protein bobap3 1 197 7.10E-17 2 7 G860 GI-7446541 T14474 MADS box protein ap3 - broccoli 1 197 7.10E-17 2 7 G860 GI-309576 homologue of Arabidopsis Agamous-like gene 1 187 8.40E-17 3 7 G860 GI-542192 PQ0770 floral homeotic protein ZAG2 - maiz... 1 187 8.40E-17 3
17 G860 GI-2252482 putative MADS domain transcription factor 1 218 1.70E-16 1
17 G860 GI-19386 TDR6 1 183 1.90E-16 2
17 G860 GI-481512 S38778 TDR6 protein - tomato (fragment) 1 183 1.90E-16 2
17 G860 GI-542029 S23731 TDR6 protein - tomato (fragment) 1 183 1.90E-16 2
17 G860 GI-1418272 AGL15 type 2 1 217 2.20E-16 1
17 G860 GI-7446561 T07869 MADS box protein AGL15 (type 2) - rape 1 217 2.20E-16 1
17 G860 GI-939783 MADS box protein 1 215 3.60E-16 1
Table 2
G860 GI-1362198 S55768 homeotic protein ZAG1 - maize (frag... 1 215 3.60E-16 1
G860 GI-7489788 T03409 MADS box protein - maize (fragment) 1 215 3.60E-16 1
G860 GI-14041687 putative agamous protein [Juglans regia] 1 143 3.80E-16 3
G860 GI-1362203 S55938 homeotic protein ZMM4 - maize (frag... 1 214 4.60E-16 1
G860 GI-3851333 putative MADS-domain transcription factor 1 169 5.30E-16 2
G860 GI-2286111 MADS box protein 1 213 5.90E-16 1
G860 GI-7446543 T04169 MADS box protein - rice 1 213 5.90E-16 1
G860 GI-1362196 S55772 homeotic protein TMZ1-I15a - maize ... 1 212 7.60E-16 1
G860 GI-4885036 AF134115_1 PISTILLATA protein homolog HPI2 1 211 9.70E-16 1
G860 GI-6468288 putative MADS domain transcription factor ... 1 190 1.00E-15 2
G860 GI-6465899 Apetala 1 protein 1 175 1.10E-15 2
G860 GI-10880319 putative MADS-domain transcription factor ... 1 158 1.10E-15 2
G860 GI-793902 ZEM1 1 210 1.20E-15 1
G860 GI-793904 ZEM2 1 210 1.20E-15 1
G860 GI-793906 ZEM3 1 210 1.20E-15 1
G860 GI-1076827 S54209 ZEM1 protein - maize (fragment) 1 210 1.20E-15 1
G860 GI-1362195 555770 homeotic protein TMZ1-I20a - maize ... 1 210 1.20E-15 1
G860 GI-1362197 555771 homeotic protein TMZ1-I5a - maize (... 1 210 1.20E-15 1
G860 GI-1362208 556754 ZEM1 protein - maize (fragment) 1 210 1.20E-15 1
G860 GI-1362209 556755 ZEM2 protein - maize (fragment) 1 210 1.20E-15 1
G860 GI-3253153 MADS-box protein 1 191 1.60E-15 2
G860 GI-16549066 putative MADS-domain transcription factor ... 1 185 1.70E-15 2
G860 GI-16549064 putative MADS-domain transcription factor ... 1 168 2.00E-15 2
G860 GI-695319 MADS box protein 1 194 2.00E-15 2
G860 GI-722400 MADS box protein 1 194 2.00E-15 2
G860 GI-3023537 CMB2_DIACA MADS BOX PROTEIN CMB2 1 194 2.00E-15 2
G860 GI-7446538 T10715 MADS-box protein CMB2 - clove pink 1 194 2.00E-15 2
G860 GI-5764265 AF147248_1 ASAPETALA3/TM6-B 1 153 2.10E-15 3
G860 GI-13442960 AF095645_1 MADS box protein nmadsl [Oryza ... 1 207 2.60E-15 1
G860 GI-17933450 MADS-box protein [Brassica napus] 1 207 2.60E-15 1
G860 GI-5764255 AF147243_1 ASAPETALA3 TM6-B 1 153 2.70E-15 3
G860 GI-8163962 AF230710_1 MADS box containing protein PI ... 1 183 2.70E-15 2
G860 GI-887577 MADS box regulatory protein 1 188 3.40E-15 2
G860 GI-1046274 MADS box regulatory protein 1 188 3.40E-15 2
G860 GI-5764279 AF147255_1 ASAPETALA3 TM6 1 153 3.40E-15 3
G860 GI-1944532 homeotic protein 1 205 4.20E-15 1
Table 2
17 G860 GI-3253151 MADS-box protein 1 205 4.20E-15 1
17 G860 GI-17933452 MADS-box protein [Brassica napus] 1 205 4.20E-15 1
17 G860 GI-5764275 AF147253_1 ASAPETALA3/TM6 1 153 4.30E-15 3
17 G860 GI-5764277 AF147254_1 ASAPETALA3/TM6 1 153 5.40E-15 3
17 G860 GI-5764283 AF147257_1 ASAPETALA3/TM6 1 153 5.40E-15 3
17 G860 GI-5764285 AF147258_1 ASAPETALA3/TM6 1 153 5.40E-15 3
17 G860 GI-15487774 AF398752_1 ASAPETALA3/TM6 [Calycadenia mul... 1 153 5.40E-15 3
17 G860 GI-15487776 AF398753_1 ASAPETALA3/TM6 [Centromadia pun... 1 153 5.40E-15 3
17 G860 GI-15487780 AF398755_1 ASAPETALA3/TM6 [Madia sativa] 1 153 5.40E-15 3
17 G860 GI-5764269 AF147250_1 ASAPETALA3/TM6-B 1 153 6.80E-15 3
17 G860 GI-602904 SLM3 1 180 9.00E-15 2
17 G860 GI-5764273 AF147252_1 ASAPETALA3/TM6 1 150 1.10E-14 3
17 G860 GI-17933454 MADS-box protein [Brassica napus] 1 201 1.10E-14 1
17 G860 GI-5764259 AF147245_1 ASAPETALA3/TM6-B 1 153 1.40E-14 3
17 G860 GI-2507631 putative MADS box transcription factor PrM... 1 200 1.40E-14 1
17 G860 GI-7484486 T10777 probable MADS box protein MADS7 - M... 1 200 1.40E-14 1
17 G860 GI-7414885 M14 [Zea mays] 1 158 1.90E-14 2 . 17 G860 GI-15022157 MADS box protein-like protein NGL9 [Medica... 1 197 3.00E-14 1
K 1 G860 GI-4218171 MADS-box protein, GDEF2 1 195 4.90E-14 1
17 G860 GI-5825623 AF180364_1 DEFICIENS homolog DEF1 1 195 4.90E-14 1
17 G860 GI-5825625 AF180365_1 DEFICIENS homolog DEF2 1 195 4.90E-14 1
17 G860 GI-2252478 putative MADS domain transcription factor 1 143 5.70E-14 2
17 G860 GI-3170462 APETALA3 homolog RfAP3-1 1 154 8.50E-14 2
17 G860 GI-2252520 putative MADS domain transcription factor 1 149 1.60E-13 3
19 G2347 BI931517 BI931517 EST551406 tomato flower, 8 mm to p... 3 221 5.30E-31 2
19 G2347 BI928213 BI928213 EST548102 tomato flower, 3 - 8 mm ... 2 221 5.60E-31 2
19 G2347 BI929558 BI929558 EST549447 tomato flower, 3 - 8 mm ... 1 220 1.00E-30 2
19 G2347 AW096676 AW096676 EST289856 tomato mixed elicitor, B... 2 221 2.50E-30 2
19 G2347 BE058432 BE058432 sn16a06.y1 Gm-c1016 Glycine max cD... 1 193 4.20E-29 2
19 G2347 BE435668 BE435668 EST406746 tomato breaker fruit, TI... 3 191 6.80E-29 2
19 G2347 AW933950 AW933950 EST359793 tomato fruit mature gree... 3 191 1.40E-28 2
19 G2347 BM732448 BM732448 sal77d10.y1 Gm-c1061 Glycine max c... 1 205 1.60E-28 2
19 G2347 AMSPB1 X92369 A.majus mRNA for squamosa-promoter b... 2 200 1.80E-28 2
19 G2347 BG525285 BG52528548-3 Stevia field grown leaf cDNA ... 2 197 5.70E-28 2
19 G2347 L38193 L38193 BNAF1025E Mustard flower buds Brassi... 1 328 4.50E-27 1
19 G2347 BG455868 BG455868 NF068F05PL1 F1045 Phosphate starved... 1 198 6.30E-27 2
Table 2
G2347 BF634536 BF634536 NF060C08DT1 F1065 Drought Medicago ... 1 183 9.70E-25 2
G2347 AMSBP2 X92079 A.majus mRNA for squamosa-promoter b... 2 171 1.10E-24 2
G2347 BG123992 BG123992 EST469638 tomato shoot/meristem Ly... 3 181 1.20E-24 2
G2347 AMA011623 AJ011623 Antirrhinum majus mRNA for squamos... 1 180 1.40E-24 2
G2347 BG097153 BG097153 EST461672 potato leaves and petiol... 3 184 1.70E-24 2
G2347 BI930428 BI930428 EST550317 tomato flower, 8 mm to p... 2 175 6.70E-24 2
G2347 AMA011622 AJ011622 Antirrhinum majus mRNA for squamos... 3 172 1.00E-23 2
G2347 BF650908 BF650908 NF098D09EC1F1076 Elicited cell cul... 1 168 1.40E-23 2
G2347 BF482644 BF482644 WHE2301-2304_A21_A21ZS Wheat pre-a... 2 176 1.60E-23 2
G2347 BF202242 BF202242 WHE0984_D01_G02ZS Wheat pre-anthes... 2 176 1.70E-23 2
G2347 AW747167 AW747167 WS1_66_F11.b1_A002 Water-stressed ... 1 170 2.30E-23 2
G2347 BG442540 BG442540 GA Ea0017G06f Gossypium arboreum ... 2 182 2.50E-23 2
G2347 BF626329 BF626329 HVSMEa0018F24f Hordeum vulgare see... 1 170 3.20E-23 2
G2347 BG593787 BG593787 EST492465 cSTS Solanum tuberosum c... 3 183 4.00E-23 2
G2347 AW932595 AW932595 EST358438 tomato fruit mature gree... 2 186 5.50E-23 2
G2347 BM140427 BM140427 WHE0483_c08_e15zS Wheat Fusarium g... 1 171 5.60E-23 2
G2347 ZMA011618 AJ011618 Zea mays mRNA for SBP-domain prote... 3 174 1.20E-22 2
G2347 BE596165 BE596165 PI1_50_D04.b1_A002 Pathogen induce... 2 169 1.60E-22 2
G2347 BG595750 BG595750 EST494428 cSTS Solanum tuberosum c... 3 164 1.70E-22 2
G2347 BE804992 BE804992 ss34h10.y1 Gm-c1061 Glycine max cD... 2 159 1.80E-22 2
G2347 RICRMSOA L34039 Oryza sativa manganese superoxide di... -1 174 3.40E-22 2
G2347 BI958783 BI958783 HVSMEn0016J12f Hordeum vulgare rac... 3 172 4.10E-22 2
G2347 BG789832 BG789832 sae56b07.y1 Gm-c1051 Glycine max c... 2 164 5.30E-22 2
G2347 BE922255 BE922255 EST426024 potato leaves and petiol... 1 164 7.20E-22 2
G2347 ZMA011614 AJ011614 Zea mays mRNA for SBP-domain prote... 1 180 1.90E-21 2
G2347 BI099345 BI099345 IP1_37_H10.b1_A002 Immature pannic... 3 177 2.00E-21 2
G2347 BE022435 BE022435 sm85h04.y1 Gm-c1015 Glycine max cD... 1 174 2.10E-21 2
G2347 BM732098 BM732098 sal72h03.y1 Gm-c1061 Glycine max c... 1 171 2.50E-21 2
G2347 BI925786 BI925786 EST545675 tomato flower, buds 0-3 ... 2 159 2.60E-21 2
G2347 AI443033 AI443033 sa31a08.y1 Gm-c1004 Glycine max cD... 3 171 3.20E-21 2
G2347 AW433271 AW433271 sh54g07.y1 Gm-d 015 Glycine max cD... 1 171 3.40E-21 2
G2347 BE440574 BE440574 sp47b09.y1 Gm-c1043 Glycine max cD... 1 171 3.60E-21 2
G2347 AQ273505 AQ273505 nbxb0030O03f CUGI Rice BAC Library... -1 171 3.70E-21 2
G2347 BM359177 BM359177 GA Ea0017G06r Gossypium arboreum ... 2 182 4.60E-21 2
G2347 AMA011621 AJ011621 Antirrhinum majus mRNA for squamos... 1 165 6.10E-21 2
G2347 ZMA011616 AJ011616 Zea mays mRNA for SBP-domain prote... 2 173 2.20E-20 2
Table 2
G2347 ZMA011615 AJ011615 Zea mays mRNA for SBP-domain prote... 2 174 2.30E-20 2
G2347 BM094278 BM094278 sah27d12.y1 Gm-c1036 Glycine max c... 2 187 4.00E-20 2
G2347 Bl 138891 BI138891 F118P50Y Populus flower cDNA libra... 1 169 5.00E-20 2
G2347 BM408208 BM408208 EST582535 potato roots Solanum tub... 1 152 6.70E-20 2
G2347 AW398794 AW398794 EST309294 L. pennellii trichome, C... 2 157 7.80E-20 2
G2347 ZMA011617 AJ011617 Zea mays mRNA for SBP-domain prote... 3 167 8.90E-20 2
G2347 ZMU89496 U89496 Zea mays ligulelessl protein (ligule... 3 160 8.90E-20 2
G2347 BM408035 BM408035 EST582362 potato roots Solanum tub... 1 152 9.30E-20 2
G2347 OSJN00201 AL663000 Oryza sativa chromosome 4 clone OS... -1 176 1.20E-19 2
G2347 AW331087 AW331087 707047A12.X1 707 - Mixed adult tis... 3 158 1.60E-19 2
G2347 OSJN00060 AL606633 Oryza sativa chromosome 4 clone OS... 2 176 1.60E-19 2
G2347 AC 108762 AC108762 Oryza sativa chromosome 9 clone OS... -2 165 1.80E-19 2
G2347 BF728335 BF728335 1000060H02.x1 1000 - Unigene I fro... 1 158 2.20E-19 2
G2347 AP004115 AP004115 Oryza sativa chromosome 2 clone OJ... -1 168 2.70E-19 2
G2347 BE325341 BE325341 NF120H09ST1 F1009 Developing stem M... 2 183 4.80E-19 2
G2347 AP004765 AP004765 Oryza sativa chromosome 8 clone P0... 3 171 9.40E-19 2
G2347 AP004324 AP004324 Oryza sativa chromosome 6 clone OJ... -2 169 1.40E-18 2
G2347 H0421 H08 AL442117 Oryza sativa genomic DNA, chromoso... 1 157 2.50E-18 2
G2347 AF451895 AF451895 Zea mays LIGULELESS1 (Ig1) gene, c... 3 157 4.00E-18 2
G2347 BE023083 BE023083 sm90e08.y1 Gm-c1015 Glycine max cD... 1 141 4.20E-18 2
G2347 AI657269 AI657269 486092G09.y1 486 - leaf primordia ... 1 170 6.00E-18 2
G2347 BE057470 BE057470 sm58e10.y1 Gm-c1028 Glycine max cD... 1 160 6.40E-18 2
G2347 OSJN00090 AL606685 Oryza sativa chromosome 4 clone OS... -2 157 6.60E-18 2
G2347 BE057780 BE057780 sn07b01.y1 Gm-c1016 Glycine max cD... 3 220 1.30E-15 1 G2347 AP004744 AP004744 Oryza sativa chromosome 6 clone OS... 1 150 1.40E-15 2
G2347 BH659801 BH659801 BOMFC96TF BO_2_3_KB Brassica olera... 2 215 2.20E-15 1
G2347 BI071295 BI071295 C054P79U Populus strain T89 leaves... -2 124 3.30E-15 2
G2347 AW091803 AW091803 EST284983 tomato mixed elicitor, B... 2 215 4.70E-15 1
G2347 BE805023 BE805023 ss35d09.y1 Gm-c1061 Glycine max cD... 2 116 6.60E-14 2
G2347 BE060783 BE060783 HVSMEg0013F15f Hordeum vulgare pre... 3 201 7.70E-14 1
G2347 BI436257 BI436257 EST539018 cSTE Solanum tuberosum c... 3 199 1.10E-13 1
G2347 BE499739 BE499739 WHE0975_G04_M07ZS Wheat pre-anthes... 1 200 1.40E-13 1
G2347 BI919686 BI919686 EST539621 potato microtubers, in v... 3 199 1.40E-13 1
G2347 AW982253 AW982253 HVSMEg0002l10f Hordeum vulgare pre... 2 197 1.70E-13 1
G2347 BH693623 BH693623 BOMPV21TR BO_2_3_KB Brassica olera... 3 194 3.50E-13 1
G2347 AW694881 AW694881 NF081A09ST1 F1068 Developing stem M... 1 128 3.80E-13 2
CM
J OJ OJ J OJ OJ o o o σ o o o o o 0 0 0) 0) 0) 0) 0) 0) 0) 00 00 00 00 00 T- T- O O O O O O O O O O O O
LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU LU
O O O P p P P O O O O o o O O O o o o P O P P O O P P P O O O m m co o) O) CO CO CO CD 1- CO rs. o oo m oo 1- oo m rs- co rr cM m r"s. co
ι- CM CM CM P rs. τ- τ- 7- CM Ln rs. τ- ^ rr LO LO LO CO CD I- DO CO CM OJ C CO rr CΛ τ- - s-1 rr" ^ co m cβ co rf co cjo o ^ m co oj 'i- co rs. m is. rr cM co co m cM cn oo oo cn oo m co co rr co P rr cM oo oo oo cM oo oo cM oo co rs. cM rs. rs. cD CD CD co ω rs. cD co cD co m m m m m m m m m m rr rr
CN
Figure imgf000216_0001
rs. rs- rs- r. is. |s. rs. r. rs. rs. rs. rs. |s. r. r. rs. rs rs. s- r-- i-- r. r. | . rs. r^ rr rr r rr rf rf rr rr rr rr rr rr rr rr rr rr rr rr rr rr rf rf rr rr rr rr rr rr rr rr rr rr rr rr rf rf co co co oo co co co co co co co co co co co co co co oo co cO co co co co co co co co co co co co co o co o CMoOJoCMoCMoCMoCMoCMoOJoOJoOJoOJoCMoCMoOJoOIoCMoCMoCNoOIoCMoCMoCMoCMoCNoCMoCMoCMoC oOIoCΛIoCMoCMoOIoCMoCMoCM
0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) 0) Table 2
19 G2347 BI925725 BI925725 EST545614 tomato flower, buds 0-3 ... 1 147 2.80E-07
19 G2347 BH580426 BH580426 BOGOB55TF BOGO Brassica oleracea g... -2 141 3.90E-07
19 G2347 AV835022 AV835022 AV835022 K. Sato unpublished cDNA ... 3 134 1.30E-06
19 G2347 BG447166 BG447166 GA Eb0040N14f Gossypium arboreum ... 2 140 1.60E-06
19 G2347 BF485120 BF485120 WHE1793_F08_L15ZS Wheat pre-anthes... 2 137 2.70E-06
19 G2347 AC 108759 AC108759 Oryza sativa chromosome 9 clone OS... -2 146 4.60E-06
19 G2347 C72011 C72011 C72011 Rice panicle at flowering sta... 1 129 7.40E-06
19 G2347 BG791002 BG791002 sae73a09.y1 Gm-c1064 Glycine max c... -3 128 8.40E-06
19 G2347 ZMA011619 AJ011619 Zea mays mRNA for SBP-domain prote... 3 141 1.20E-05
19 G2347 C72359 C72359 C72359 Rice panicle at flowering sta... 2 125 2.30E-05
19 G2347 AI813122 AI813122 23C10 Pine Lambda Zap Xylem librar... 2 120 6.40E-05
19 G2347 BF596468 BF596468 su51f06.y1 Gm-c1069 Glycine max cD... 1 129 8.30E-05
19 G2347 AV395862 AV395862 AV395862 Chlamydomonas reinhardtii... 3 1 18 0.00011
19 G2347 BI470983 BI470983 sah92a05.y1 Gm-c1050 Glycine max c... 3 1 17 0.00014
19 G2347 BVCOXII X55297 B.vulgaris coxll gene for cytochrome... 2 134 0.00015
19 G2347 AB008450 AB008450 Beta vulgaris mitochondrial rps7 g... 2 134 0.00017
19 G2347 AP000396S2 AP000397 Beta vulgaris mitochondrial genomi... 2 134 0.00019 κ» 19 G2347 AW509094 AW509094 sh30a06.y1 Gm-c1017 Glycine max cD... 2 116 0.00021
£ 19 G2347 AQ289632 AQ289632 nbxb0019K20f CUGI Rice BAC Library... 2 130 0.00023
19 G2347 BG557285 BG557285 EM1_42_A05.b1_A002 Embryo 1 (EM1) ... 1 116 0.00036
19 G2347 AV396403 AV396403 AV396403 Chlamydomonas reinhardtii... 2 118 0.00041
19 G2347 BE499423 BE499423 WHE0974_F04_L08ZS Wheat pre-anthes... 2 120 0.00077
19 G2347 BH689424 BH689424 BOMPQ46TR BO_2_3_KB Brassica olera... -1 123 0.0016
19 G2347 AV393371 AV393371 AV393371 Chlamydomonas reinhardtii... 3 118 0.0018
19 G2347 BE400548 BE400548 AWB004.H05F000328 ITEC AWB Wheat M... 1 106 0.004
19 G2347 BH481141 BH481141 BOGXV25TR BOGX Brassica oleracea g... -1 119 0.0052
19 G2347 AI726550 AI726550 BNLGHJ6118 Six-day Cotton fiber Go... 2 117 0.008
19 G2347 BE518391 BE518391 WHE0815_F09_L17ZS Wheat vernalized... 2 109 0.012
19 G2347 BE607271 BE607271 NXCI_034_H03_F NXCI (Nsf Xylem Com... 1 102 0.012
19 G2347 BF587332 BF587332 FM1_35_B04.b1_A003 Floral-Induced ... 3 104 0.019
19 G2347 BG594131 BG594131 EST492809 cSTS Solanum tuberosum c... 1 113 0.021
19 G2347 BG320129 BG320129 Zm03_05c03_A Zm03_AAFC_ECORC_cold_. -2 1 13 0.027
19 G2347 BG947454 BG947454 IP1_4_F04.b1_A002 Immature pannicl... 3 109 0.051
19 G2347 BG947455 BG947455 IP1_4_F05.b1_A002 Immature pannicl... 3 109 0.051
19 G2347 BF483589 BF483589 WHE2335_E11_J21ZS Wheat pre-anthes... 1 109 0.051
19 G2347 BG 124241 BG 124241 EST469875 tomato shoot/meristem Ly... 2 110 0.057
Table 2
19 G2347 BG651519 BG651519 sad47h02.y1 Gm-c1075 Glycine max c... 3 105 0.058
19 G2347 BM111172 BM111172 EST558708 potato roots Solanum tub... 2 108 0.097
19 G2347 AU083475 AU083475 AU083475 Rice panicle (longer than... 2 104 0.1 1
19 G2347 BH230073 BH230073 1006155H05.X1 1006 - RescueMu Grid... -1 97 0.27
19 G2347 BH475782 BH475782 BOHPK53TF BOHP Brassica oleracea g... 3 103 0.35
19 G2347 BE920196 BE920196 EST423965 potato leaves and petiol... 2 99 0.47
19 G2347 AU162331 AU162331 AU162331 Rice cDNA from immature I... 1 97 0.49
19 G2347 C91611 C91611 C91611 Rice panicle (longer than 10c... 3 90 0.66
19 G2347 BE460553 BE460553 EST411972 tomato breaker fruit, TI... 2 94 0.86
19 G2347 BF617209 BF617209 HVSMEc0015A05f Hordeum vulgare see... 1 97 0.9
19 G2347 BG300299 BG300299 HVSMEb0012A01f Hordeum vulgare see... -1 92 0.99
19 G2347 GI-1183864 squamosa-promoter binding protein 2 -1 171 1.50E-31 3
19 G2347 GI-2130004 S62361 squamosa-promoter binding protein 2... -1 171 1.50E-31 3
19 G2347 GI-6094241 SBP2_ANTMA SQUAMOSA-PROMOTER BINDING PROTE.. -1 171 1.50E-31 3
19 G2347 GI-1183866 squamosa-promoter binding protein 1 -1 200 1.10E-30 2
19 G2347 GI-2130003 S62360 squamosa-promoter binding protein 1... -1 200 1.10E-30 2
19 G2347 GI-6094239 SBP1_ANTMA SQUAMOSA-PROMOTER BINDING PROTE.. -1 200 1.10E-30 2 κ» 19 G2347 GI-5931643 squamosa promoter binding protein-homologue 5 -1 180 4.10E-27 2
^ 19 G2347 GI-5931641 squamosa promoter binding protein-homologue 4 -1 172 8.20E-27 2
19 G2347 GI-5931786 SBP-domain protein 5 -1 174 3.40E-25 2
19 G2347 GI-5931778 SBP-domain protein 1 -1 180 1.30E-23 2
19 G2347 GI-5931639 squamosa promoter binding protein-homologue 3 -1 165 1.50E-23 2
19 G2347 GI-5931782 SBP-domain protein 3 -1 173 9.10E-23 2
19 G2347 GI-5931780 SBP-domain protein 2 -1 174 1.60E-22 2
19 G2347 GI-5931784 SBP-domain protein 4 -1 167 3.70E-22 2
19 G2347 GI-1914845 ligulelessl protein -1 160 6.60E-22 2
19 G2347 GI-6016502 LG1_MAIZE LIGULELESS1 PROTEIN -1 160 6.60E-22 2
19 G2347 GI-7489786 T04328 ligulelessl protein - maize -1 160 6.60E-22 2
19 G2347 GI-17940734 AF451895_1 LIGULELESS1 [Zea mays] -1 160 6.60E-22 2
19 G2347 GI-8468036 Similar to Arabidopsis thaliana chromosome... -1 175 1.40E-21 2
19 G2347 GI-5931790 SBP-domain protein 7 -1 165 3.40E-12
19 G2347 GI-9087308 orf 102a [Beta vulgaris var. altissima] -1 134 6.60E-09
19 G2347 GI-9838446 orf 102a [Beta vulgaris var. altissima] -1 134 6.60E-09
19 G2347 G1-5931788 SBP-domain protein 6 -1 141 1.20E-07
19 G2347 GI-7209500 S-locus pollen protein -1 60 0.83
21 G722 BH477453 BH477453 BOGXM90TR BOGX Brassica oleracea gen... -2 557 1.90E-61
o o co m m m m co P P CΛ o) oo CD IO m in m m rT rT rr rT rr rr rr rr CO CO CO CO CO CO CO
LU LU LU LU LU LU LU LU LU LU LU LL LU llJ LU LU LU llJ UJ IjJ llJ LU LU LU Lϋ LLJ LU Lϋ LL LU LU LU LU ω LU o o o o o o o o o o o o o o o rf o O O CM CO CO CO CO CΛ CO CO o o o o o o o o o o o
O o CO o 1— o CΛ o O) rf CM CD C CJO rr -r- CM CO P 'i-;
T- cM - ^ oj j co -r- rr rs. rr Λ T^ oi rr' T^ oJ i^ i rs; i^ -r rr" fs." rsi -' rr Os' c CO cb T-' ^ CM' rf cb cjo c» m oi oj -^ -i- ^ P oj oj |s. cn m -f- o co rs. ->- cM T- rr oo o o cM co cD cn co co co cM CM O) oo i- T- -i- i- cM M oj c i- T- i- o o 0) rr o) 0) '- CM m c rs. oo m co cM m oo T- o) 0 0 0 oo o
W W CVI C\I W C\I C\I W N W W C\l '- r- C '- ι- C\I W N W W '- W C\I C\I C\I C\l ι- C\l '- ι- C\I N τ- W
Figure imgf000219_0001
t oo rs. j0D (o 2
Figure imgf000219_0002
CO
CM CM CM CM CM CM CM CM CM OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ CM CM CM CM CM OJ OJ OJ OJ OJ OJ OJ OJ CM Cr-M- CisM. CrMs. CisM. CrsM. CrsM. CrsM. CrsM. CrsM. Ci>M. CrsM. CsM. CrM. OrsJ. OrsJ. OsJ. OrsJ. Or--J OisJ. Oi>J- OrsJ. OrsJ. CrMs C|M. C|sM- C|sM. C|sM» CrsM. CM CM CM CM CM CM CM OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO CM CM CM OI CM CM C C CM CM CM CM CM C CΛI CM OJ OJ OJ -ι- OJ OJ OJ ->- CO CM - -^ -ι- CM CM 'i- 'i- ->- CM CM
rs. rs. m m m m rr cM τ- o o o o cΛ oo oo oo oo oo oo co co m oi '>- τ- 'i- O O O 00 00 00 00
W N Cl « N CM CM N W N N N N '- τ- ι- r ι- ι- τ- τ- r τ- ι- τ- r ι- 1- T- 1- O O O o m m m LU LU Lu m LU LU Lu iu m L Lu L m Lu Lu LU Lu u m Lu m o o O O O O O O O P P o o o o o o o p P P P P P P P o o σ> co T- cn oo Lo cM co in in co CO CM CO CD CO CD rr pr-. T- CpO Prr in oo co r- cn cn co CO IS O i- oo co co rs. cn co i- rs. CM rr rr r-- m CM rr T-" CO CO CO rr CO i- rs. O) i- CM CM C0 ->- CO
(D N Ol CO CO CO iD CO oo p co i- p rr rr o rr co m rr co rr cΛ O oo oo cM CΛ i- cM co cM T-
CΛ CM co oo oo oo co ι>- p oo cn cΛ P oo ι^ ι^ oo rs. m m rs- m m σ) m cM oo oo rs. -ι- P co co co 0S-0 CM ; o τ- CM l- τ- l- τ- τ- *- ,- τ- ,- τ- ι- ,- -^ ,- τ- ι- 1- C\l - »- ι- ι- l- τ- l- l- l- ι- *- ι- τ- τ- 0' ,-
C - C CM CM C O c CM τ- " ^ CO CO -ι- O C CM CO C -. ^ c sg ^ - CM CM '- ι- τ- CM
Figure imgf000220_0001
^ ° 52 co c5, ιn ι^ cΛ r; ls!! ? o co co „ m co m r. rr ,. co ^ cn C m rs. 2 co co oo l-n S | O?2 S8 SW ^^ 55 S^ 'oJ SS Ng NS PO ^2 I^O SS SU) oSo oog mS cmM rsr cror g coo Sw 5cS co Kg c §^ cό^ co S S
§ LU 0 § 0 O XX --22 !!--Jj 22 §§ 0θ mm 22 χx 5P2 0θ ιι 00 22 00 0θ 22 22 N θθ 22 -ijj 22 lLjj LLuu 55 xx 33 Oθ u; m m m < m < m ω m m ω m o co m cQ m ω co < < < co < co < cQ < cQ
OJ OJ OJ OJ OJ CM CM OJ OJ CM CM CM OJ OJ OJ OJ OJ OJ OJ OJ CM CM CM OO CM CM CN CM C OI CM CM CM CM CM OI M CM C C C CM C C CM CM N rs- s. ιs. rs. r. rs. s r. r. rs. rs. rs. r. rs. rs. rs. rs. s. |. ι . ιs» fs. |. fs. rs. rs. rs. rs. r. rs. OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO
CΛI CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM OJ OJ CM CM CM OI CM CM CM OJ OJ CM CM CM CM CM CM
c Po coD oco pco po mo mo mo om mo c^M rSr cmn ocoo .O_ T- m _ .co_ _rf_ rf
LU LU LU LU LU LU LU LU LU LU LU O O O m . o o o o O O O O O O O O O O O O O O o o o o o P o o o o o o o o o o co rs. cD O) oo cM rs. o) oj cq rs. O O O o P P d d d p d o d o
T-' ^ cϋ w i ' ^ ^ ^ cvi xi ri d d d i P o d d o d d rf O o o o o
Figure imgf000221_0001
rs. rs. r. rs. rs. rs. rs. OOOOOOO
Figure imgf000221_0002
OOOOOOOOOOOO
r¥ δ 3 - C « CO Λ1 M m CΛ CO Cθ m rs. -^ ^ -ι- CO rt m cr3 C rs. rt l. CO CΛ rt σ) P 'i- CM CM CM CO oo oo oo cM CM CM CO CO CO CO rt rt rt rf rt rr m m m cO . |s. rs. 00 00 00 O) O) O) P O) i O -• τ- ^ ^ c o d O O O O P P O O O O O P O O O O O O O O O O O O O O O
|s- oo co P o m -ι- ~ m o co o m - o o o o r-- rr cΛ co oo σ) c)o co rr o m co rf _, oo oo oo _
O i- ^ '^ T- i- o g O '- '^ '- P ^ -^ ^ -^ '^ O i- P O O O O O O - O O O gj P O O oJ
IN 0 - W '- 7 C0 C0 τ- C\I N 7 N τ- 7 t? 7 7 ι- ' 0 N C0 (? N r- r. ^ -- 0 N θ N C -
Figure imgf000222_0001
5Z? cd ff co
S 5 m8
S 0?
DQ <
Figure imgf000222_0002
I CM CM CM OJ CM CM CM OJ OJ CM CM CM CM CM CM CM OJ OJ CM CM CM CM CM OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ
|s. r. r. h. rs. |s. s. rs. rs. rs. rs. rs. r. |s- rs. |s. rs. |s. s. r. r. rs. r i>s r. ι, ιs. ι^ OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO
CM C CM CM CM OJ CM OI C CM C CM OJ OJ OJ OJ OJ CM CM CM CM CM CΛI W M W C W CM CM W m m m CM CO CM CM CM CM CM CM CO CO CO CM CM CM CM CM CM CO
Figure imgf000223_0001
o ) S S § cn o) oo o) 0) oo o) oo o g r; C r- σ) - ι- m m rr P CD co co oo co co co P CD Co cM
° ? ° ° ° 0) 0) 0) 0) 0) 0) 03 O1 0) 2 N N N σ> σ) cn oo co rs. oo co ιs. rs. co ιn ιn ιn co cD co cD
Figure imgf000223_0002
o rt m - m oo o rr cM o rs. oo CM O
_s _. _ „ _ cn rs. CM oo cΛ CΛ rr σ> σ) θ θ r-- co o oo m rr co rs. rs. cn
52 I-: Lf> ^ Ln - _ ι- oo c rr s. P° _ „, oo -- co o) rt rt LO rf co io rs. o cM rr ^ -- CM co cn cn co Λ cp s. 2 co co ^ co t 't !s; ri P 5S n ->- ι>. cn o o rs. o ^ σ rf cM CM Co s. cn cM oo oo o
* 5δ ^ S P τT - CO m rt P Hc C; 5S θθ m m cθ rt rt rs. rt rt rt e CO P rs. rt O rt Λ σ) m CD ; 5S is- 5? P CΛ S P c2 ι-- rr r D θ cΛ ^ D m cM Λ θ '^ σ) P σ P -ι- -r- - cM θj oj oo o co o 5^ 52 Lθ θJ c) m S c P τt cM ^ Ln p ω -t c m m co c ^ c τ- ^ σ CΛ co cM m m co θ '>- CM
2 S iS S n i' S « l2 n '* ^ 2 t; ^ ,- '- ,- '- '- ,- ,- ,- T- '- '- 1'' io <- cM s if) D - -
0 (3 I -! Ul lli 2 lLl O -l -. 2 5 2 2 - — --. -i. --. --. _L --. _L _L. _i. _L _L _L _L _L _L. _L _L J. _L ω __ __ _ _- __ _- _j _j _j __ < __ _- _j O O O O O O O O O O O O O O O O O O O O O
CM OJ CM CM CM CM C C C CM OJ OJ OJ OJ OJ C C CM CM CM CM CM CM CM OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ CM CM CM CM CM CM CM CM C OI CM CM CM CM CM CM CM CM CM CM OJ OJ OJ OJ CM CM CM CM C CM CM CM CM CM CM CM CM CM o rs-or .or--or-oP or.ors.ors.o|s.ors.ors.o|s.ors.o|s.ors.ors.ors.ors.o|s.ors.ors.ors.ors.ors.ors-o|s.or--ors^oooooooo Table 2
21 G722 GI-4519671 transfactor 3 66 0.99 2
21 G722 GI-11034542 hypothetical protein-similar to Arabidopsi... 2 74 0.998 2
21 G722 GI-12225928 unnamed protein product [Zea mays] 3 81 0.998 1
21 G722 GI-12328569 hypothetical protein [Oryza sativa] 3 59 0.9993 4
23 G1493 BH687265 BH687265 BOMOL63TR BO_2_3_KB Brassica olerace... -3 381 6.20E-67 2
23 G1493 AB060130 AB060130 Zea mays ZmRR8 mRNA for response reg... 1 323 1.10E-65 5
23 G1493 AC107226 AC107226 Oryza sativa chromosome 3 clone OSJN... 1 336 1.60E-63 5
23 G1493 BH592456 BH592456 BOGDT29TR BOGD Brassica oleracea gen... -3 548 8.90E-62 2
23 G1493 BH450431 BH450431 BOHJY73TF BOHJ Brassica oleracea gen... -3 361 3.80E-57 2
23 G1493 AB071695 AB071695 Zea mays ZmRRIO mRNA for response re... 1 324 1.70E-55 4
23 G1493 BH707101 BH707101 BOMBY56TR BO_2_3_KB Brassica olerace... 1 548 5.90E-50 1
23 G1493 AW719680 AW719680 LjNEST8C3r Lotus japonicus nodule Ii... 1 267 6.10E-50 3
23 G1493 BE660997 BE660997 312 GmaxSC Glycine max cDNA, mRNA se... 1 301 1.70E-47 3
23 G1493 AB062095 AB062095 Zea mays ZmRR9 mRNA for response reg... 3 272 2.20E-46 4
23 G1493 AW596288 AW596288 sj01e07.y1 Gm-c1032 Glycine max cDNA... 1 300 2.30E-45 4
23 G1493 AP004552 AP004552 Oryza sativa chromosome 6 clone OJ10... 1 281 4.10E-44 4
23 G1493 BM326218 BM326218 PIC1_72_C05.b1_A002 Pathogen-infecte... 1 213 9.30E-42 3
Is) 23 G1493 AP004094 AP004094 Oryza sativa chromosome 2 clone OJ16... -1 256 2.80E-41 4 Is)
Ui 23 G1493 AP003975 AP003975 Oryza sativa chromosome 2 clone OJ10... -3 256 4.40E-41 4
23 G1493 BM337985 BM337985 MEST220-B11 3 ISUM5-RN Zea mays cDN... -1 199 4.90E-40 3
23 G1493 BG450692 BG450692 NF102C07DT1 F1052 Drought Medicago tr... 3 295 7.70E-39 2
23 G1493 BE205050 BE205050 EST397726 KVO Medicago truncatula cD... 2 160 6.00E-38 4
23 G1493 D41804 D41804 RICS4626A Rice shoot Oryza sativa cDNA... 3 193 6.90E-38 3
23 G1493 BM407041 BM407041 EST581368 potato roots Solanum tuber... 3 274 7.20E-38 2
23 G1493 BM336723 BM336723 MEST198-A02.T3 ISUM5-RN Zea mays cDN... -2 271 1.70E-37 2
23 G1493 BF632520 BF632520 NF039A08DT1 F1054 Drought Medicago tr... 3 242 2.50E-37 2
23 G1493 BM334786 BM334786 MEST142-B06.T3 ISUM5-RN Zea mays cDN... -2 199 4.60E-37 3
23 G1493 BE661025 BE6610252-B11 GmaxSC Glycine max cDNA, mRNA ... 3 423 2.40E-36 1
23 G1493 BM337275 BM337275 MEST205-E11 3 ISUM5-RN Zea mays cDN... -3 199 3.10E-36 3
23 G1493 AP004087 AP004087 Oryza sativa chromosome 2 clone OJ12... 2 304 1.90E-35 2
23 G1493 BF068683 BF068683 St31d08.y1 Gm-c1067 Glycine max cDNA... 2 199 3.50E-34 3
23 G1493 AP004008 AP004008 Oryza sativa chromosome 6 clone OJ12... 1 168 4.20E-34 5
23 G1493 BH485050 BH485050 BOGZQ58TF BOGZ Brassica oleracea gen... 2 187 4.30E-34 3
23 G1493 AP003517 AP003517 Oryza sativa chromosome 6 clone OSJN... -3 168 1.40E-33 5
23 G1493 AW564570 A 564570 LG1_296_E01.b1_A002 Light Grown 1 (L... 2 183 1.60E-32 3
23 G1493 BH604770 BH604770 BOHSC62TF BOHS Brassica oleracea gen... 3 266 1.60E-31 2
Table 2
G1493 BM086439 BM086439 sah29a07.y1 Gm-c1036 Glycine max cDN... 1 378 2.00E-31 1
G1493 BH697183 BH697183 B0MMS16TR BO_2_3_KB Brassica olerace... 3 149 2.30E-31 4
G1493 BE353308 BE353308 EST400440 L. hirsutum trichome, Corn... 2 377 2.60E-31 1
G1493 BE600028 BE600028 PI1_78_E09.b1_A002 Pathogen induced ... 1 199 4.30E-31 3
G1493 AW691588 AW691588 NF042D10ST1 F1000 Developing stem Med... 2 160 6.40E-31 3
G1493 BG581278 BG581278 EST483011 GVN Medicago truncatula cD... 2 160 3.50E-30 3
G1493 BE214029 BE214029 HV_CEb0001 P06f Hordeum vulgare seedl... 2 185 4.00E-30 3
G1493 BE053397 BE053397 GA Ea0033l07f Gossypium arboreum 7-... 2 364 4.30E-30 1
G1493 AW677774 AW677774 WS1_11_D07.b1_A002 Water-stressed 1 ... 2 272 1.20E-28 2
G1493 BG525366 BG525366 47-6 Stevia field grown leaf cDNA St... 3 167 1.80E-28 3
G1493 BG240289 BG240289 OV1_20_B11.b1_A002 Ovary 1 (OV1) Sor... 1 268 3.40E-28 2
G1493 AW396912 AW396912 sg64g09.y1 Gm-c1007 Glycine max cDNA... -3 207 3.60E-28 2
G1493 AI974630 AI974630 T113082e KV2 Medicago truncatula cDN... 2 240 1.40E-27 2
G1493 BF631773 BF631773 NF007B07DT1 F1058 Drought Medicago tr... 2 188 1.60E-27 2
G1493 AW560754 AW560754 EST315802 DSIR Medicago truncatula c... -2 219 4.90E-27 2
G1493 BJ181458 BJ181458 BJ181458 normalized full length cDNA... 1 195 2.20E-26 2
G1493 BI098295 BI098295 IP1_30_F04.b1_A002 Immature pannicle... 1 248 2.70E-26 2
G1493 BG310033 BG310033 HVSMEc0016B23f Hordeum vulgare seedl... 3 187 7.80E-25 2
G1493 BM660787 BM660787 952041 H09.x1 952 - BMS tissue from W... 1 185 1.60E-24 2
G1493 BF587105 BF587105 FM1_32_C05.b1_A003 Floral-Induced Me... 3 187 3.30E-24 2
G1493 BH457884 BH457884 BOGHG34TF BOGH Brassica oleracea gen... 3 301 2.50E-23 1
G1493 BH681845 BH681845 BOMOG14TR BO_2_3_KB Brassica olerace... 1 301 2.90E-23 1
G1493 BM360628 BM360628 GA Ea0033l07r Gossypium arboreum 7-... 1 295 1.50E-22 1
G1493 BG129795 BG 129795 EST475441 tomato shoot/meristem Lyco... 3 200 4.70E-22 2
G1493 BI924617 BI924617 EST544506 tomato flower, buds 0-3 mm... 3 200 7.00E-22 2
G1493 BG560474 BG560474 RHIZ2_74_H01.b1_A003 Rhizome2 (RHIZ2... 2 199 1.00E-21 2
G1493 BE597633 BE597633 PI1_72_B12.b1_A002 Pathogen induced ... 1 199 1.00E-21 2
G1493 BM492554 BM492554 NXRV_028_A07_F NXRV (Nsf Xylem Root ... -3 211 1.90E-21 2
G1493 C74559 C74559 C74559 Rice panicle shorter than 3cm O... 3 189 2.40E-21 2
G1493 AL372190 AL372190 MtBA49C02F1 MtBA Medicago truncatula... 1 188 1.50E-20 2
G1493 BE205320 BE205320 EST397996 KVO Medicago truncatula cD... 2 188 1.60E-20 2
G1493 AI443904 AI443904 sa44h06.y1 Gm-c1004 Glycine max cDNA... 1 274 4.10E-20 1
G1493 BE661800 BE661800 158 GmaxSC Glycine max cDNA, mRNA se... 1 186 1.40E-19 2
G1493 BF633922 BF633922 NF066E04DT1F1034 Drought Medicago tr... 3 188 1.40E-18 2
G1493 BG049455 BG049455 OV1_20_B11.g1_A002 Ovary 1 (OV1 ) Sor... 3 176 1.70E-18 2
G1493 BH667506 BH667506 BOMNU54TF BO_2 3_KB Brassica olerace... -1 251 5.80E-18 1
Table 2
23 G1493 BG523436 BG523436 31 -73 Stevia field grown leaf cDNA S... 3 168 1.10E-17 2
23 G1493 BE353296 BE353296 EST400428 L. hirsutum trichome, Corn... 3 155 1.60E-17 2
23 G1493 BM888844 BM888844 952071 E09.x1 952 - BMS tissue from W... -2 158 3.90E-17 2
23 G1493 AU084218 AU084218 AU084218 Cryptomeria japonica inner ... 1 151 1.90E-16 2
23 G1493 AC027038 AC027038 Oryza sativa chromosome 10 clone OSJ... -1 186 4.30E-16 3
23 G1493 AZ135727 AZ135727 Gm_UMb001_190_P19R UMN Soybean BAC L. -2 132 4.80E-16 2
23 G1493 AL381868 AL381868 MtBC03E05F1 MtBC Medicago truncatula... 3 160 5.30E-16 2
23 G1493 AL367049 AL367049 MtBA11 H10F1 MtBA Medicago truncatula... -2 147 5.40E-16 2
23 G1493 BM086086 BM086086 sah35c12.y1 Gm-c1036 Glycine max cDN... 3 150 1.10E-15 2
23 G1493 BM736391 BM736391 952050A04.x1 952 - BMS tissue from W... -3 149 1.30E-15 2
23 G1493 BG592527 BG592527 EST491205 cSTS Solanum tuberosum cDN... 2 165 2.00E-15 2
23 G1493 BM736413 BM736413 952050D06.x1 952 - BMS tissue from W... -1 150 3.80E-15 2
23 G1493 AL367833 AL367833 MtBA19F01 F1 MtBA Medicago truncatula... 1 152 5.10E-15 2
23 G1493 BM520832 BM520832 sal31a11.y1 Gm-c1059 Glycine max cDN... 2 108 1.30E-13 3
23 G1493 BM325142 BM325142 PIC1_40_B10.b1_A002 Pathogen-infecte... 3 143 1.10E-12 2
23 G1493 BH538825 BH538825 BOGKD72TF BOGK Brassica oleracea gen... 3 139 6.90E-12 2
23 G1493 BH575345 BH575345 BOHIU91TF BOHI Brassica oleracea gen... -3 189 3.20E-11 1
Is) 23 G1493 BM736421 BM736421 952050E03.x1 952 - BMS tissue from W... 1 131 6.60E-11 2 Is) Ul 23 G1493 BG789991 BG789991 sae61f06.y1 Gm-d 064 Glycine max cDN... 1 116 2.60E-09 2
23 G1493 AW944749 AW944749 EST336799 tomato flower buds 3-8 mm,... 1 160 4.20E-08 1
23 G1493 BG123738 BG123738 EST469384 tomato shoot meristem Lyco... 1 159 1.60E-07 1
23 G1493 BI974308 BI974308 sai99a11.y1 Gm-c1065 Glycine max cDN... 2 107 1.30E-06 2
23 G1493 BH450678 BH450678 BOHRL03TF BOHR Brassica oleracea gen... -1 102 3.00E-06 2
23 G1493 BH744755 BH744755 gt41f02.b1 BoBudsOI Brassica olerace... 2 143 3.30E-06 1
23 G1493 AW694254 AW694254 NF074C08ST1 F1065 Developing stem Med... 1 150 4.00E-06 1
23 G1493 BF070582 BF070582 st22e02.y1 Gm-c1065 Glycine max cDNA... -3 148 4.50E-06 1
23 G1493 C72732 C72732 C72732 Rice panicle at flowering stage... 1 141 5.70E-06 1
23 G1493 BM187690 BM187690 saj87g12.y1 Gm-c1074 Glycine max cDN... 2 140 6.30E-06 1
23 G1493 BG522035 BG522035 17-58 Stevia field grown leaf cDNA S... 2 139 7.50E-06 1
23 G1493 BH527190 BH527190 BOGZ095TF BOGZ Brassica oleracea gen... -2 102 8.10E-06 2
23 G1493 BI405862 BI405862 147F09 Mature tuber lambda ZAP Solan... 2 144 8.30E-06 1
23 G1493 BM326134 BM326134 PIC1_66_B06.b1_A002 Pathogen-infecte... 2 138 1.10E-05 1
23 G1493 AW689536 AW689536 NF021 H03ST1 F1000 Developing stem Med... 2 147 1.30E-05 1
23 G1493 BH459360 BH459360 BOHQU06TR BOHQ Brassica oleracea gen... 1 102 1.50E-05 2
23 G1493 Bl178237 BI178237 EST519182 cSTE Solanum tuberosum cDN... 3 98 2.90E-05 2
23 G1493 AW650154 AW650154 EST328608 tomato germinating seedlin... 3 137 3.00E-05 1
Table 2
23 G1493 BI271101 BI271101 NF050B06FL1 F1057 Developing flower M... 1 101 3.30E-05 2
23 G1493 BH532040 BH532040 BOHRI33TF BOHR Brassica oleracea gen... 1 102 3.80E-05 2
23 G1493 BG606967 BG606967 WHE2492_G02_M04ZS Triticum monococcu... 1 98 4.10E-05 2
23 G1493 Bl179657 BI179657 EST520602 cSTE Solanum tuberosum cDN... 3 99 5.30E-05 2
23 G1493 BM325391 BM325391 PIC1_44_A04.b1_A002 Pathogen-infecte... 1 128 0.00013
23 G1493 AV936280 AV936280 AV936280 K. Sato unpublished cDNA Ii... -1 139 0.00015
23 G1493 BE606498 BE606498 WHE0908_C07_F14ZS Wheat 5-15 DAP spi... 2 126 0.00021
23 G1493 BI074783 BI074783 IP1_15_B09.b1_A002 Immature pannicle... 3 126 0.00022
23 G1493 AV936377 AV936377 AV936377 K. Sato unpublished cDNA Ii... -3 139 0.00022
23 G1493 AV937037 AV937037 AV937037 K. Sato unpublished cDNA Ii... -1 139 0.00022
23 • G1493 AV936494 AV936494 AV936494 K. Sato unpublished cDNA Ii... -1 139 0.00023
23 G1493 BG133615 BG133615 EST466423 tomato crown gall Lycopers... 2 126 0.00024
23 G1493 BM325806 BM325806 PIC1_53_A05.b1_A002 Pathogen-infecte... 1 125 0.00027
23 G1493 BH693119 BH693119 BOHTJ77TR BO_2_3_KB Brassica olerace... -1 134 0.00044
23 G1493 BH556049 BH556049 BOGOM92TR BOGO Brassica oleracea gen... -3 137 0.00055
23 G1493 BI469444 BI469444 sai12a08.y1 Gm-c1053 Glycine max cDN... 3 122 0.00063
23 G1493 BM134876 BM134876 WHE0452_H01_H01ZS Wheat Fusarium gra... 2 129 0.00082
23 G1493 BG644799 BG644799 EST506418 KV3 Medicago truncatula cD... 2 123 0.0011
23 G1493 BM309528 BM309528 sak63f 11.y1 Gm-c1036 Glycine max cDN... 1 125 0.0015
23 G1493 AU182322 AU182322 AU182322 Rice panicle at flowering s... 2 118 0.0017
23 G1493 AI054482 AI054482 coau0001B23 Cotton Boll Abscission Z... 3 129 0.0033
23 G1493 BH488734 BH488734 BOHLY95TF BOHL Brassica oleracea gen... -1 129 0.0056
23 G1493 AW585322 AW585322 EST306017 KV2 Medicago truncatula cD... 3 127 0.0066
23 G1493 AQ869929 AQ869929 nbeb0036A02r CUGI Rice BAC Library (... 3 128 0.0068
23 G1493 AL369717 AL369717 MtBA32H05F1 MtBA Medicago truncatula... 1 112 0.0071
23 G1493 AW218302 AW218302 EST303483 tomato radicle, 5 d post-i... 2 113 0.0079
23 G1493 AV932456 AV932456 AV932456 K. Sato unpublished cDNA Ii... 1 122 0.01
23 G1493 BH435183 BH435183 BOHM051TF BOHM Brassica oleracea gen... -1 110 0.012
23 G1493 AC084405 AC084405 Oryza sativa chromosome unknown BAC ... -1 129 0.016
23 G1493 BH633001 BH633001 1007058A08.y1 1007 - RescueMu Grid H... 1 111 0.017
23 G1493 BM498464 BM498464 952026F12.x1 952 - BMS tissue from W... 2 118 0.024
23 G1493 BI948917 BI948917 HVSMEI0011 H03f Hordeum vulgare spike... 2 117 0.025
23 G1493 BM356578 BM356578 12IV-A9 Triphysaria versicolor root-... 2 115 0.034
23 G1493 AP003831 AP003831 Oryza sativa chromosome 7 clone OJ14... -1 126 0.034
23 G1493 BF587215 BF587215 FM1_33_F04.b2_A003 Floral-Induced Me... 3 110 0.034
23 G1493 BF008920 BF008920 ss71a03.y1 Gm-c1062 Glycine max cDNA... 3 107 0.035
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CM CM CM OJ OJ OJ Ovl OJ OJ OJ OJ OJ OJ Od OJ OJ OJ OJ OJ OJ OJ OJ CM CM CM CM OJ OJ OJ OJ OI CM OJ OJ OJ OJ Table 2
23 G1493 GI-399783 GRW1_LYCES GLYCINE-RICH CELL WALL STRUCTUR.. 1 50 0.63 2
23 G1493 GI-1345532 glycine-rich protein 1 50 0.63 2
23 G1493 GI-7489007 S14979 glycine-rich protein (clone uE-7) -... 1 50 0.63 2
23 G1493 GI-10241615 hypothetical protein [Oryza sativa] 2 84 0.7 1
23 G1493 GI-18478480 AF334211_1 maturation-associated SRCMike... 2 69 0.72 1
23 G1493 GI-4456620 alpha-galactosidase 2 49 0.72 2
23 G1493 GI-13940500 AF318582_1 putative transcription factor O... 2 91 0.82 1
23 G1493 GI-12328569 hypothetical protein [Oryza sativa] 2 77 0.84 3
23 G1493 GI-3687688 response regulator protein 1 62 0.87 2
23 G1493 GI-10041875 unnamed protein product [Brassica napus] 1 62 0.87 2
23 G1493 GI-11034707 hypothetical protein-similar to Arabidopsi... 2 91 0.89 1
23 G1493 GI-12060386 response regulator 5 [Zea mays] 2 83 0.92 2
23 G1493 GI-2108258 extensin -1 44 0.92 2
23 G1493 GI-11761085 putative DNA-binding protein homolog [Oryz... 2 87 0.98 1
23 G1493 GI-11761106 putative DNA-binding protein homolog [Oryz... 2 87 0.98 1
23 G1493 GI-14994231 drought-induced protein [Retama raetam] 2 74 0.99 1
23 G1493 GI-12060388 response regulator 6 [Zea mays] 2 81 0.994 2
23 G1493 GI-12225924 unnamed protein product [Zea mays] 2 81 0.994 2
Is) Is) sO 23 G1493 GI-166376 environmental stress-induced protein 1 44 0.998 2
23 G1493 GI-7488739 T09610 environmental stress-induced protei... 1 44 0.998 2
23 G1493 GI-10241424 hypothetical protein [Oryza sativa] -3 78 0.999 1
25 G475 BH659801 BH659801 BOMFC96TF BO_2_3_KB Brassica olera... 2 389 8.00E-34 1
25 G475 AMSPB1 X92369 A.majus mRNA for squamosa-promoter b... 2 253 1.00E-33 2
25 G475 BI931517 BI931517 EST551406 tomato flower, 8 mm to p... 3 257 3.30E-33 2
25 G475 BI928213 BI928213 EST548102 tomato flower, 3 - 8 mm ... 2 257 3.50E-33 2
25 G475 AW096676 AW096676 EST289856 tomato mixed elicitor, B... 2 257 4.70E-33 2
25 G475 BE435668 BE435668 EST406746 tomato breaker fruit, TI... 3 251 1.20E-32 2
25 G475 AW933950 AW933950 EST359793 tomato fruit mature gree... 3 251 2.30E-32 2
25 G475 BI929558 BI929558 EST549447 tomato flower, 3 - 8 mm ... 1 240 3.10E-31 2
25 G475 BG455868 BG455868 NF068F05PL1 F1045 Phosphate starved... 1 219 8.20E-29 2
25 G475 BM732448 BM732448 sal77d10.y1 Gm-c1061 Glycine max c... 1 222 4.30E-28 2
25 G475 BE922255 BE922255 EST426024 potato leaves and petiol... 1 142 7.00E-28 3
Figure imgf000230_0001
25 G475 BE058432 BE058432 sn16a06.y1 Gm-c1016 Glycine max cD... 1 193 3.60E-26 2
25 G475 AMSBP2 X92079 A.majus mRNA for squamosa-promoter b... 2 191 4.30E-26 2
25 G475 BE499739 BE499739 WHE0975_G04_M07ZS Wheat pre-anthes... 1 189 5.30E-25 2
25 G475 BI071295 BI071295 C054P79U Populus strain T89 leaves... 1 187 1.40E-24 2
Table 2
G475 BF650908 BF650908 NF098D09EC1 F1076 Elicited cell cul... 1 184 1.60E-24 2
G475 BG097153 BG097153 EST461672 potato leaves and petiol... 3 180 2.20E-24 2
G475 BI930428 BI930428 EST550317 tomato flower, 8 mm to p... 2 179 6.70E-24 2
G475 BM140427 BM140427 WHE0483_c08_e15zS Wheat Fusarium g... 1 177 1.30E-23 2
G475 BI925786 BI925786 EST545675 tomato flower, buds 0-3 ... 2 177 1.50E-23 2
G475 BM408035 BM408035 EST582362 potato roots Solanum tub... 1 172 1.50E-23 2
G475 BM408208 BM408208 EST582535 potato roots Solanum tub... 1 172 1.50E-23 2
G475 BE060783 BE060783 HVSMEg0013F15f Hordeum vulgare pre... 3 183 2.20E-23 2
G475 BG595750 BG595750 EST494428 cSTS Solanum tuberosum c... 3 175 3.20E-23 2
G475 BF634536 BF634536 NF060C08DT1 F1065 Drought Medicago ... 1 177 3.60E-23 2
G475 AMA011621 AJ011621 Antirrhinum majus mRNA for squamos... 1 184 5.90E-23 2
G475 BG 123992 BG 123992 EST469638 tomato shoot/meristem Ly... 3 179 8.90E-23 2
G475 AQ273505 AQ273505 nbxb0030O03f CUGI Rice BAC Library... -1 178 1.60E-22 2
G475 AMA011622 AJ011622 Antirrhinum majus mRNA for squamos... 3 174 2.20E-22 2
G475 BF482644 BF482644 WHE2301-2304_A21_A21ZS Wheat pre-a... 2 162 2.20E-22 2
G475 BF202242 BF202242 WHE0984_D01_G02ZS Wheat pre-anthes... 2 162 2.40E-22 2
G475 BF626329 BF626329 HVSMEa0018F24f Hordeum vulgare see... 1 162 3.70E-22 2
G475 H0421 H08 AL442117 Oryza sativa genomic DNA, chromoso... 1 204 4.30E-22 2
G475 AF451895 AF451895 Zea mays LIGULELESS1 (Ig1 ) gene, c... 3 189 5.30E-22 2
G475 BG593787 BG593787 EST492465 cSTS Solanum tuberosum c... 3 171 5.70E-22 2
G475 AW747167 AW747167 WS1_66_F1 1.b1_A002 Water-stressed ... 1 168 8.50E-22 2
G475 AP004324 AP004324 Oryza sativa chromosome 6 clone OJ... -2 199 1.00E-21 2
G475 OSJN00090 AL606685 Oryza sativa chromosome 4 clone OS... -2 204 1.20E-21 2
G475 BI436257 BI436257 EST539018 cSTE Solanum tuberosum c... 3 274 1.30E-21 1
G475 RICRMSOA L34039 Oryza sativa manganese superoxide di... -1 165 1.60E-21 2
G475 BI919686 BI919686 EST539621 potato microtubers, in v... 3 274 1.60E-21 1
G475 BG582086 BG582086 EST483824 GVN Medicago truncatula ... 1 173 3.20E-20 2
G475 AP004115 AP004115 Oryza sativa chromosome 2 clone OJ... -1 182 1.00E-19 2
G475 AW929797 AW929797 EST354067 tomato flower buds 8 mm ... 1 255 2.00E-19 1
G475 AW929795 AW929795 EST354065 tomato flower buds 8 mm ... 1 255 2.30E-19 1
G475 ZMA011618 AJ011618 Zea mays mRNA for SBP-domain prote... 3 159 2.40E-19 2
G475 BM094278 BM094278 sah27d12.y1 Gm-c1036 Glycine max c... 2 178 2.70E-19 2
G475 BG525285 BG525285 48-3 Stevia field grown leaf cDNA ... 2 250 6.60E-19 1
G475 AP004765 AP004765 Oryza sativa chromosome 8 clone PO... 3 165 7.40E-19 2
G475 AC108762 AC108762 Oryza sativa chromosome 9 clone OS... -2 169 7.80E-19 2
G475 BE325341 BE325341 NF120H09ST1 F1009 Developing stem M... 2 177 7.80E-19 2
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Figure imgf000232_0001
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Figure imgf000232_0002
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m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m mm m m m Table 2
25 G475 OSJN00060 AL606633 Oryza sativa chromosome 4 clone OS... 2 191 6.00E-12
25 G475 AW982980 AW982980 HVSMEg0004L14f Hordeum vulgare pre... 3 184 6.60E-12
25 G475 ZMA011614 AJ011614 Zea mays mRNA for SBP-domain prote... 1 182 3.00E-11
25 G475 AC092557 AC092557 Oryza sativa chromosome 3 clone OS... 2 184 3.30E-11
25 G475 ZMA011615 AJ011615 Zea mays mRNA for SBP-domain prote... 2 181 3.90E-11
25 G475 ZMA011617 AJ011617 Zea mays mRNA for SBP-domain prote... 3 180 5.60E-11
25 G475 AW398794 AW398794 EST309294 L. pennellii trichome, C... 2 175 5.60E-11
25 G475 BI307968 BI307968 EST529378 GPOD Medicago truncatula... 3 174 6.00E-11
25 G475 AW331087 AW331087 707047A12.X1 707 - Mixed adult tis... 3 173 7.00E-11
25 G475 ZMA011616 AJ011616 Zea mays mRNA for SBP-domain prote... 2 179 7.30E-11
25 G475 A 932595 AW932595 EST358438 tomato fruit mature gree... 2 173 8.70E-11
25 G475 BH408621 BH408621 1007005H05.1 EL_x1 1007 - RescueMu ... 2 174 9.00E-11
25 G475 BG440913 BG440913 GA Ea0010P04f Gossypium arboreum ... 1 172 9.20E-11
25 G475 BF728335 BF728335 1000060H02.x1 1000 - Unigene I fro... 1 173 9.70E-11
25 G475 BE596165 BE596165 PI1_50_D04.b1_A002 Pathogen induce... 2 169 2.60E-10
25 G475 BE518391 BE518391 WHE0815_F09_L17ZS Wheat vernalized... 2 102 2.80E-10
25 G475 BG933421 BG933421 WS1_2_B01.b1_A002 Water-stressed 1... 2 169 3.10E-10
25 G475 BF704779 BF704779 FM1_51_D08.b1_A003 Floral-Induce ... 3 167 5.00E-10
Is)
Ui Is) 25 G475 BE057470 BE057470 sm58e10.y1 Gm-c1028 Glycine max cD... 1 166 5.50E-10
25 G475 BE806499 BE806499 ss59f10.y1 Gm-c1062 Glycine max cD... 3 166 6.60E-10
25 G475 BI099345 BI099345 IP1_37_H10.b1_A002 Immature pannic... 3 166 7.70E-10
25 G475 Bl 128093 BI128093 G070P72Y Populus cambium cDNA libr... 3 165 7.90E-10
25 G475 BM374244 BM374244 EBpi03_SQ003_B07_R IGF Barley EBpi... 3 166 8.20E-10
25 G475 BG789832 BG789832 sae56b07.y1 Gm-c1051 Glycine max c... 2 163 1.10E-09
25 G475 AC108759 AC108759 Oryza sativa chromosome 9 clone OS... -1 168 1.70E-09
25 G475 BI933324 BI933324 EST553213 tomato flower, anthesis ... 3 156 4.40E-09
25 G475 ZMA011620 AJ011620 Zea mays mRNA for SBP-domain prote... 2 157 6.20E-09
25 G475 BE804992 BE804992 ss34h10.y1 Gm-c1061 Glycine max cD... 2 154 1.10E-08
25 G475 BH580426 BH580426 BOGOB55TF BOGO Brassica oleracea g... -2 154 1.70E-08
25 G475 AI820134 AI820134 605086D06.X3 605 - Endosperm cDNA ... -3 152 2.00E-08
25 G475 AB008450 AB008450 Beta vulgaris mitochondrial rps7 g... 2 153 5.50E-08
25 G475 BI928266 BI928266 EST548155 tomato flower, 3 - 8 mm ... 145 6.90E-08
25 G475 BI927982 BI927982 EST547859 tomato flower, 3 - 8 mm ... 145 8.20E-08
25 G475 BI927983 BI927983 EST547860 tomato flower, 3 - 8 mm ... 145 9.10E-08
25 G475 BE023083 BE023083 sm90e08.y1 Gm-c1015 Glycine max cD... 145 9.70E-08
25 G475 BG630869 BG630869 cC-esflcLEI_5D24d1 Tomato flower Ii... 143 1.70E-07
Table 2
G475 BVCOXII X55297 B.vulgaris coxll gene for cytochrome... 2 148 1.70E-07 1
G475 BI925725 BI925725 EST545614 tomato flower, buds 0-3 ... 1 141 1.80E-07 1
G475 AP000396S2 AP000397 Beta vulgaris mitochondrial genomi... 2 148 2.20E-07 1
G475 BF422188 BF422188 FM1_12_H07.b1_A003 Floral-Induced ... 1 139 3.70E-07 1
G475 AU091662 AU091662 AU091662 Rice panicle at flowering... 2 137 7.60E-07 1
G475 C72011 C72011 C72011 Rice panicle at flowering sta... 1 136 1.30E-06 1
G475 BF596468 BF596468 su51f06.y1 Gm-c1069 Glycine max cD... 1 133 1.50E-06 1
G475 BG791002 BG791002 sae73a09.y1 Gm-c1064 Glycine max c... -3 134 1.70E-06 1
G475 BG129294 BG129294 EST474940 tomato shoot meristem Ly... 3 135 2.10E-06 1
G475 AV835022 AV835022 AV835022 K. Sato unpublished cDNA ... 3 132 2.20E-06 1
G475 C72359 C72359 C72359 Rice panicle at flowering sta... 2 130 5.50E-06 1
G475 BF587332 BF587332 FM1_35_B04.b1_A003 Floral-Induced ... 3 128 6.60E-06 1
G475 BH689424 BH689424 BOMPQ46TR BO_2_3_KB Brassica olera... -1 129 7.60E-06 1
G475 BG947454 BG947454 IP1_4_F04.b1_A002 Immature pannicl... 3 125 1.00E-05 1
G475 BG947455 BG947455 IP1_4_F05.b1_A002 Immature pannicl... 3 125 1.00E-05 1
G475 AI726550 AI726550 BNLGHi6118 Six-day Cotton fiber Go... 2 123 3.30E-05 1
G475 BE499423 BE499423 WHE0974_F04_I_08ZS Wheat pre-anthes... 2 119 4.90E-05 1
G475 AW509094 AW509094 sh30a06.y1 Gm-c1017 Glycine max cD... 2 120 5.10E-05 1
G475 BG447166 BG447166 GA Eb0040N14f Gossypium arboreum ... 2 118 6.60E-05 1
G475 BM111172 BM111172 EST558708 potato roots Solanum tub... 2 120 7.20E-05 1
G475 BF483589 BF483589 WHE2335_E11_J21ZS Wheat pre-anthes... 1 118 7.40E-05 1
G475 BG320129 BG320129 Zm03_05c03_A Zm03_AAFC_ECORC_cold_ -2 120 8.20E-05 1
G475 BG557285 BG557285 EM1_42_A05.b1_A002 Embryo 1 (EM1) ... 1 117 8.80E-05 1
G475 BI470983 BI470983 sah92a05.y1 Gm-c1050 Glycine max c... 3 116 0.00012 1
G475 AQ289632 AQ289632 nbxb0019K20f CUGI Rice BAC Library... 2 117 0.00019 1
G475 L38193 L38193 BNAF1025E Mustard flower buds Brassi... 1 112 0.00035 1
G475 AV630386 AV630386 AV630386 Chlamydomonas reinhardtii... 3 116 0.0004 1
G475 AV395862 AV395862 AV395862 Chlamydomonas reinhardtii... 3 111 0.0004 1
G475 AI813122 AI813122 23C10 Pine Lambda Zap Xylem librar... 2 110 0.00051 1
G475 BF485120 BF485120 WHE1793_F08_L15ZS Wheat pre-anthes... 2 113 0.00093 1
G475 BE400548 BE400548 AWB004.H05F000328 ITEC AWB Wheat M.. 1 109 0.00095 1
G475 BH230073 BH230073 1006155H05.X1 1006 - RescueMu Grid... -1 107 0.0011 1
Figure imgf000234_0001
G475 AV396403 AV396403 AV396403 Chlamydomonas reinhardtii... 2 111 0.0013 1
G475 AU083475 AU083475 AU083475 Rice panicle (longer than... 2 111 0.0017 1
G475 BH481141 BH481141 BOGXV25TR BOGX Brassica oleracea g... -1 114 0.0019 1
G475 AV393371 AV393371 AV393371 Chlamydomonas reinhardtii... 3 111 0.0026 1
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CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM CM W CM CM CM CM CM CM W Table 2
G475 BG551638 BG551638 sad40h10.y1 Gm-c1075 Glycine max c... -3 82 0.62 1
G475 BM441017 BM441017 EBed02_SQ002_C23_R IGF Barley EBed... 3 93 0.62 1
G475 AY023649 AY023649 Oryza sativa microsatellite MRG597... -2 83 0.62 1
G475 PAB296706 AJ296706 Picea abies ATC microsatellite DNA... -2 83 0.64 1
G475 E15302 E15302 Oryza sativa microsatellite marker. ... 2 83 0.64 1
G475 AY023507 AY023507 Oryza sativa microsatellite MRG583... -3 83 0.64 1
G475 D49319 D49319 RICS16541A Rice green shoot Oryza sa... -2 87 0.65 1
G475 BE059683 BE059683 sn35e01.y1 Gm-c1016 Glycine max cD... -3 82 0.66 1
G475 AY023443 AY023443 Oryza sativa microsatellite MRG576... -3 83 0.66 1
G475 C22335 C22335 C22335 Miyagawa-wase satsuma mandari... -1 82 0.66 1
G475 BM092791 BM092791 sah18h08.y3 Gm-c1086 Glycine max c... -3 82 0.67 1
G475 AY023054 AY023054 Oryza sativa microsatellite MRG537... 2 82 0.69 1
G475 AY023052 AY023052 Oryza sativa microsatellite MRG537... -3 82 0.7 1
G475 PNI297295 AJ297295 Populus nigra microsatellite DNA, ... -3 92 0.7 1
G475 HVC39SAT Z50100 H.vulgare GAA-satellite DNA. 7/1996 2 81 0.71 1
G475 AW424183 AW424183 sh62a09.y1 Gm-d 015 Glycine max cD... -3 81 0.72 1
G475 BE059388 BE059388 sn30d03.y1 Gm-c1016 Glycine max cD... -1 81 0.72 1
G475 E15301 E15301 Oryza sativa microsatellite marker. ... 2 83 0.74 1
G475 AU078614 AU078614 AU078614 Rice callus Oryza sativa ... 2 90 0.74 1
G475 AU164357 AU164357 AU164357 Rice panicle (between 3cm... 1 91 0.74 1
G475 BE807092 BE807092 ss12a11.y1 Gm-c1047 Glycine max cD... -1 81 0.75 1
G475 BI941982 BI941982 se37e10.y1 Gm-d 015 Glycine max cD... -1 83 0.75 1
G475 BE920196 BE920196 EST423965 potato leaves and petiol... 2 93 0.76 1
G475 AU183116 AU183116 AU183116 Rice panicle (between 3cm... 1 91 0.77 1
G475 BG629564 BG629564 cC-esflcLEL29D05a1 Tomato flower I... -2 90 0.78 1
G475 AU164424 AU164424 AU164424 Rice panicle (between 3cm... 3 91 0.78 1
G475 BE123203 BE123203 945024G05.Y2 945 - Mixed adult tis... -3 81 0.78 1
G475 BM523640 BM523640 sam86f04.y2 Gm-c1087 Glycine max c... 3 88 0.78 1
G475 BF265362 BF265362 HV_CEa0011 M06f Hordeum vulgare see... -1 95 0.79 1
G475 AU164325 AU164325 AU164325 Rice panicle (between 3cm... 1 91 0.81 1
G475 AG022234 AG022234 Oryza sativa DNA, 3' flanking sequ... 1 80 0.82 1
G475 BF481726 BF481726 FM1_21_F04.b1_A003 Floral-Induced ... 3 90 0.84 1
G475 AY023447 AY023447 Oryza sativa microsatellite MRG577... -3 81 0.84 1
G475 AY023485 AY023485 Oryza sativa microsatellite MRG581... -3 81 0.84 1
G475 AQ868916 AQ868916 nbeb0032L01 r CUGI Rice BAC Library... 3 93 0.86 1
G475 AI649852 AI649852 496007D05.x1 496 - stressed shoot ... 2 89 0.86 1
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25 G475 AW 185870 AW185870 se60f07.y1 Gm-c1019 Glycine max cD... 2 89 0.96 1
25 G475 BI893336 BI893336 sai64g07.y1 Gm-c1068 Glycine max c... 3 89 0.97 1
25 G475 BE923417 BE923417 EST427186 potato leaves and petiol... -3 78 0.97 1
25 G475 AY023662 AY023662 Oryza sativa microsatellite MRG598... -2 78 0.97 1
25 G475 AW423351 AW423351 sh06f08.y1 Gm-c1016 Glycine max cD... -2 84 0.97 1
25 G475 AF344155 AF344155 Oryza sativa subsp. indica microsa... 2 87 0.97 1
25 G475 AY023053 AY023053 Oryza sativa microsatellite MRG537... 2 78 0.97 1
25 G475 AI727524 AI727524 BNLGHi8326 Six-day Cotton fiber Go... -2 91 0.97 1
25 G475 AY022326 AY022326 Oryza sativa microsatellite MRG465... 1 78 0.97 1
25 G475 BG275427 BG275427 NXSI_141_G05_F NXSI (Nsf Xylem Sid... -3 78 0.98 1
25 G475 AY022346 AY022346 Oryza sativa microsatellite MRG467... 3 78 0.98 1
25 G475 AW 102527 AW102527 sd60d06.y1 Gm-c1008 Glycine max cD... -3 78 0.98 1
25 G475 AU070270 AU070270 AU070270 Rice mature leaf Oryza sa... 1 91 0.98 1
25 G475 BI941734 BI941734 sc88e06.y1 Gm-c1019 Glycine max cD... -1 78 0.98 1
25 G475 BE360639 BE360639 DG1_64_E02.g1_A002 Dark Grown 1 (D... -2 81 0.98 1
25 G475 BE459517 BE459517 EST414809 tomato developing/immatu... -1 90 0.99 1
25 G475 AW278406 AW278406 sf43f12.y1 Gm-c1009 Glycine max cD... -1 78 0.99 1
25 G475 AU162331 AU162331 AU162331 Rice cDNA from immature I... 1 88 0.99 1
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Ui -4 25 G475 AF324782 AF324782 Pinus taeda clone PtTX4093 microsa... -1 87 0.991 1
25 G475 BI941857 BI941857 sd55g08.y1 Gm-c1016 Glycine max cD... -2 78 0.991 1
25 G475 AU069234 AU069234 AU069234 Rice callus Oryza sativa ... -3 87 0.991 1
25 G475 BI471607 BI471607 sag22e01.y1 Gm-c1080 Glycine max c... -3 78 0.992 1
25 G475 BF266221 BF266221 HV_CEa0014l05f Hordeum vulgare see... -2 91 0.994 1
25 G475 AW101674 AW101674 Sd68f03.y1 Gm-c1008 Glycine max cD... 1 78 0.994 1
25 G475 BI784657 BI784657 saf57f09.y3 Gm-c1078 Glycine max c... 1 89 0.996 1
25 G475 AZ132429 AZ132429 OSJNBb0062P10r CUGI Rice BAC Libra... -2 90 0.996 1
25 G475 BI945310 BI945310 sb55b03.y1 Gm-c1018 Glycine max cD... -3 84 0.997 1
25 G475 BG855067 BG855067 1024041 F10.y2 C. reinhardtii CC-16... -2 91 0.997 1
25 G475 BH642615 BH642615 1008042D08.x1 1008 - RescueMu Grid... 2 88 0.997 1
25 G475 AF261696 AF261696 Rubus alceifolius microsatellite C... -3 86 0.998 1
25 G475 BM269508 BM269508 MEST410-C02.univ ISUM5-RN Zea mays... 2 89 0.998 1
25 G475 Bl 177442 Bl 177442 EST518387 cSTE Solanum tuberosum c... -2 80 0.998 1
25 G475 AC108500 AC108500 Oryza sativa chromosome 5 clone OJ... -2 92 0.999 1
25 G475 AF344158 AF344158 Oryza sativa subsp. indica microsa... -2 86 0.999 1
25 G475 BM736626 BM736626 952045B08.y1 952 - BMS tissue from... 1 88 0.999 1
25 G475 BG842681 BG842681 MEST34-G10.T7-1 ISUM3-TL Zea mays ... 2 89 0.9991 1
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Figure imgf000239_0001
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L2 oo t n ∞ -- oo 5 rt m ιo c o o c m o rt co rt co oo rt r o co rs. rs- o co c c)o c θ rr m 2 o S '- S c o f; b „ - n S g N eo o N ((0 ^ (o co ιι) ^ o ^ o ^ n t ιΛ _ϊ rs- CM o CD o r-- ^ o cp τ- _j- c o rr co o rr -r- --- _- ^ -- rr m cΛ rr --- -ι- -- c)o -- -- rs. oo σ) J ^ S n ^ W S 2 ^ o o c cji o e gι n n n n n τ- - oθ (!) n n c - n n _ n cf m C [> o O co cS - cM -- ^ o -- -- o cn o) 0) 0) 0) P O rr rs. o 0) cn r o) 0) o oo m c cM θ o M o o si oθ co rs. -- CM CD -- M P m m m m τ- co rs. -- m oo m σ) σ CD _ _ _ _ 0 _ Q. S O (3 _ _ - _ - _ _ _ - - - - - _ _ _ _ J. J _ - - - - - _ _- __ _- __ < < < < < _J < C0 O O O O O O O O O O O O O O O O O O O O O m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m r. rs. rs- s. rs- rs. rs. rs. |s. rs. ιs f. rs. rs. |s. ι. rs. s. rs. rs. rs. |s. rs. r. rs. rs- ιs. rt rt rt rt rt rf rt rt rf rr rr rr rt rt rf rt rf rt rt rt rt rt rt rt rr rr rr rr rr rr rr rr rr rr rr ooooooooooooooooooooooooooo
Figure imgf000239_0002
oooooooo
Lp Lp m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m m
CM OJ OJ OJ OJ CM CM OJ OJ J J J OJ J OJ OJ OJ OJ OJ 04 0J OJ OJ OJ OJ OJ J OJ OJ OJ OJ OJ OJ OI J OJ Table 2
G475 GI-6539594 hypothetical protein 1 77 0.058 1
G475 GI-6077100 S-locus protein 1 1 85 0.15 1
G475 GI-6469127 farnesylated protein 1 71 0.21 1
G475 GI-2224518 ORF54d -2 65 0.25 1
G475 GI-7520715 T07354 NADH dehydrogenase (ubiquinone) cha... -2 65 0.25 1
G475 GI-7524924 ORF54d [Chlorella vulgaris] -2 65 0.25 1
G475 GI-1161252 nucleosome assembly protein 1 85 0.28 1
G475 GI-2129840 S60892 nucleosome assembly protein 1 - soy... 85 0.28 1
G475 GI-1778378 NAPIPs 85 0.29 1
G475 G1-7488816 T06807 nucleosome assembly protein 1 - gar... 85 0.29 1
G475 GI-18855014 AC087599_25 hypothetical protein [Oryza sa... 2 64 0.32 1
G475 GI-14329812 putative nucleosome assembly protein 1 [At... 74 0.38 1
G475 GI-15451587 AC090483_1 Putative transposable element [... 79 0.44 1
G475 GI-4106378 calcium-binding protein 78 0.47 1
G475 GI-2598571 putative 60 0.66 1
G475 GI-13194674 AF325720_1 calreticulin-like protein [Penn... 76 0.75 1
G475 GI-7657876 SIAH1 protein [Brassica napus] 79 0.82 1
G475 GI-15864565 SIAH1 protein [Brassica napus] 79 0.82 1
G475 GI-14485577 AF320029_1 heme oxygenase 2 [Lycopersicon ... 79 0.84 1
G475 GI-14029025 AC079853_19 Unknown protein [Oryza sativa] 81 0.84 1
G475 GI-20783 legumin 80 0.88 1
G475 GI-81913 S00336 legumin B LegJ precursor - garden pea 80 0.88 1
G475 GI-126170 LEGJ_PEA LEGUMIN J PRECURSOR 80 0.88 1
G475 GI-2224513 ORF63 -2 56 0.95 1
G475 GI-7515416 T07349 hypothetical protein 63 - Chlorella... -2 56 0.95 1
G475 GI-7524919 ORF63 [Chlorella vulgaris] -2 56 0.95 1
G475 GI-3129939 hypothetical protein 1 71 0.96 1
G475 GI-4539666 AF061282_12 hypothetical protein 1 74 0.98 1
G475 GI-2224405 ORF49b -2 55 0.98 1
G475 GI-7515336 T07242 hypothetical protein 49b - Chlorell... -2 55 0.98 1
G475 GI-7524812 ORF49b [Chlorella vulgaris] -2 55 0.98 1
G475 GI-17027265 AC090713_6 putative parathymosin [Oryza sa... 1 75 0.98 1
G475 GI-21021 pseudogene region -3 54 0.994 1
G475 GI-2224361 ORF46a -2 54 0.994 1
G475 GI-7515316 T07198 hypothetical protein 46a - Chlorell... -2 54 0.994 1
G475 GI-7524768 ORF46a [Chlorella vulgaris] -2 54 0.994 1
rs rs- o oo o σ, σ, D g. g g S cn o) cn o) 0) 0) 0 L" "j s» gj ^" cn
0) 0) 0) 0) 0) o o o o o o o d d d d d
CM
CN U
Figure imgf000241_0001
O B r r
CO rf i- CO O OO CM CΛ O Cn rt T- oo cΛ co o cM CΛ m co cn p co co
O- CM CΛ CO CM P CO CM CM CΛ OO CM OO co rs. τ- oo cM CM - rs. rs. co m cM rt
CO OO rr i- CM CO m oO OO CM O OO O co co oo p m τ- rr co co m cM cn cM
0) '- τ- n N C N - - - CO W t
OOOOOOOOOOOOO mrs. mrs. mr. mrs- rs. mrs. mrs. mrs. rms. ims. mfs. mis. mrs. rt rf rt rf rt rt rt rf rt rf rt rt rr
OOOOOOOOOOOOO
m m m m m m m m mm m m m

Claims

What is claimed is:
1. An isolated or recombinant polynucleotide comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence of SEQ ID NO: 8 (G2010), SEQ ID NO: 2 (G590), SEQ ID NO: 4 (G1760), SEQ ID NO: 6 (G1820), or SEQ ID NO: 20 (G2347), or a complementary nucleotide sequence thereof.
2. A transgenic plant, which plant comprises a recombinant polynucleotide comprising a nucleotide sequence from the group consisting of:
(a) a nucleotide sequence encoding a polypeptide comprising a sequence selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 and 26, or a complementary nucleotide sequence thereof;
(b) a nucleotide sequence encoding a polypeptide comprising a conservatively substituted variant of a polypeptide of (a);
(c) a nucleotide sequence comprising a sequence selected from those of SEQ ID NO: 1 , 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25, or a complementary nucleotide sequence thereof;
(d) a nucleotide sequence comprising silent substitutions in a nucleotide sequence of
(c);
(e) a nucleotide sequence which hybridizes under stringent conditions to a nucleotide sequence of one or more of: (a), (b), (c), or (d);
(f) a nucleotide sequence comprising at least 15 consecutive nucleotides of a sequence of any of (a)-(e);
(g) a nucleotide sequence comprising a subsequence or fragment of any of (a)-(f), which subsequence or fragment encodes a polypeptide having a biological activity that modifies a plant's flowering phenotype;
(h) a nucleotide sequence having at least 40% sequence identity to a nucleotide sequence of any of (a)-(g);
(i) a nucleotide sequence having at least 85% sequence identity to a nucleotide sequence of any of (a)-(g); (j) a nucleotide sequence which encodes a polypeptide having at least 40% sequence identity to a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26; (k) a nucleotide sequence which encodes a polypeptide having at least 85% sequence identity to a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26; (1) a nucleotide sequence which encodes a polypeptide containing a conserved domain having at least 65% sequence identity to a conserved domain of a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26;
(m) a nucleotide sequence which encodes a plant transcription factor comprising a fragment having 6 consecutive amino acids from a region of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 outside of the conserved domain; (n) a nucleotide sequence having at least 85% sequence identity to (m); (o) a nucleotide sequence which encodes a plant transcription factor comprising a fragment having 8 consecutive amino acids from a region of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 outside of the conserved domain; and (p) a nucleotide sequence having at least 85% sequence identity to (o).
3. The transgenic plant of claim 2, further comprising a constitutive, inducible, or tissue-specific promoter operably linked to said nucleotide sequence.
4. An isolated or recombinant polynucleotide comprising a nucleotide sequence from the group consisting of: (a) a nucleotide sequence encoding a polypeptide comprising a sequence selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 and 26, or a complementary nucleotide sequence thereof;
(b) a nucleotide sequence encoding a polypeptide comprising a conservatively substituted variant of a polypeptide of (a); (c) a nucleotide sequence comprising a sequence selected from those of SEQ ID NO:
1 , 3, 5, 7, 9, 11 , 13, 15, 17, 19, 21, 23 and 25, or a complementary nucleotide sequence thereof;
(d) a nucleotide sequence comprising silent substitutions in a nucleotide sequence of
(c); (e) a nucleotide sequence which hybridizes under stringent conditions to a nucleotide sequence of one or more of: (a), (b), (c), or (d); (f) a nucleotide sequence comprising at least 15 consecutive nucleotides of a sequence of any of (a)-(e);
(g) a nucleotide sequence comprising a subsequence or fragment of any of (a)-(f , which subsequence or fragment encodes a polypeptide having a biological activity that modifies a plant's flowering phenotype;
(h) a nucleotide sequence having at least 40% sequence identity to a nucleotide sequence of any of (a)-(g);
(i) a nucleotide sequence having at least 85% sequence identity to a nucleotide sequence of any of (a)-(g); (j) a nucleotide sequence which encodes a polypeptide having at least 40% sequence identity to a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26; (k) a nucleotide sequence which encodes a polypeptide having at least 85% sequence identity to a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26; (1) a nucleotide sequence which encodes a polypeptide containing a conserved domain having at least 65% sequence identity to a conserved domain of a polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26;
(m) a nucleotide sequence which encodes a plant transcription factor comprising a fragment having 6 consecutive amino acids from a region of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 outside of the conserved domain; (n) a nucleotide sequence having at least 85% sequence identity to (m);
(o) a nucleotide sequence which encodes a plant transcription factor comprising a fragment having 8 consecutive amino acids from a region of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26 outside of the conserved domain; and (p) a nucleotide sequence having at least 85% sequence identity to (o).
5. The isolated or recombinant polynucleotide of claim 4, further comprising a constitutive, inducible, or tissue-specific promoter operably linked to the nucleotide sequence.
6. A cloning or expression vector comprising the isolated or recombinant polynucleotide of claim 4.
7. A transformed cell comprising the vector of claim 6.
8. A transformed cell comprising the isolated or recombinant polynucleotide of claim 4.
9. A transgenic plant comprising the isolated or recombinant polynucleotide of claim 4.
10. A composition comprising two or more different polynucleotides of claim 4.
1 1. An isolated or recombinant polypeptide comprising a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotide of claim 4.
12. A plant ectopically expressing an isolated polypeptide of claim 11.
13. A method for producing a plant having a modified flowering phenotype, the method comprising altering the expression of the isolated or recombinant polynucleotide of claim 4 or the expression levels or activity of a polypeptide of claim 1 1 in a plant, thereby producing a modified plant, and selecting a plant for an alteration in flowering phenotype, thereby providing the modified plant with a modified flowering phenotype.
14. The method of claim 13, wherein the plant is selected by comparing the timing of the onset of reproductive development, the duration of the phase in which floral meristems are initiated, the duration of time for which floral organs persist, or the number of flowers generated on a plant to a control or wild-type plant.
15. A method of identifying a factor that is modulated by or interacts with a polypeptide encoded by a polynucleotide of claim 4, the method comprising: (a) expressing a polypeptide encoded by the polynucleotide in a plant; and (b) identifying at least one factor that is modulated by or interacts with the polypeptide.
16. The method of claim 15, wherein the identifying is performed by detecting binding by the polypeptide to a promoter sequence, or detecting interactions between an additional protein and the polypeptide in a yeast two hybrid system.
17. The method of claim 15, wherein the identifying is performed by detecting expression of a factor by hybridization to a microarray, subtractive hybridization or differential display.
18. A method of identifying a molecule that modulates activity or expression of a polynucleotide or polypeptide of interest, the method comprising:
(a) placing the molecule in contact with a plant comprising the polynucleotide or polypeptide encoded by the polynucleotide of claim 4; and,
(b) monitoring one or more of: (i) expression level of the polynucleotide in the plant; (ii) expression level of the polypeptide in the plant; (iii) modulation of an activity of the polypeptide in a plant; or (iv) modulation of an activity of the polynucleotide in the plant.
19. An integrated system, computer or computer readable medium comprising one or more character strings corresponding to a polynucleotide of claim 4, or to a polypeptide encoded by the polynucleotide.
20. The integrated system, computer or computer readable medium of claim 19, further comprising a link between said one or more sequence strings and a plant trait associated with modified plant flowering phenotype.
21. A method of identifying a sequence homologous to one or more polynucleotides of claim 4, or one or more polypeptides encoded by the polynucleotides, the method comprising: (a) providing a sequence database; and,
(b) querying the sequence database with one or more target sequences corresponding to the one or more polynucleotides or to the one or more polypeptides to identify one
60 or more members of the database that display sequence similarity or homology to one or more of the target sequences.
22. The method of claim 21 , wherein the querying comprises aligning one or more of the target sequences with one or more of the members of the sequence database.
23. The method of claim 22, wherein the querying comprises identifying one or more of the members of the database that meet a user-selected identity criteria with one or more of the target sequences.
24. The method of claim 21 , further comprising linking the one or more polynucleotides or encoded polypeptides of claim 4 to a plant trait associated with modified plant flowering phenotype.
25. The method of claim 21 , wherein the target sequence contains at least 5 consecutive amino acids of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 or 26.
26. The method of claim 21 , wherein the target sequence contains at least 15 consecutive nucleotides of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 or 25.
27. A plant comprising altered expression levels of the isolated or recombinant polynucleotide of claim 4.
28. A plant comprising altered expression levels or the activity of the isolated or recombinant polypeptide of claim 11.
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