WO2002074927A3 - High accuracy protein identification - Google Patents

High accuracy protein identification Download PDF

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Publication number
WO2002074927A3
WO2002074927A3 PCT/US2002/008450 US0208450W WO02074927A3 WO 2002074927 A3 WO2002074927 A3 WO 2002074927A3 US 0208450 W US0208450 W US 0208450W WO 02074927 A3 WO02074927 A3 WO 02074927A3
Authority
WO
WIPO (PCT)
Prior art keywords
sets
protein identification
high accuracy
identification
data
Prior art date
Application number
PCT/US2002/008450
Other languages
French (fr)
Other versions
WO2002074927A2 (en
Inventor
Thang T Pham
Original Assignee
Ciphergen Biosystems Inc
Thang T Pham
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciphergen Biosystems Inc, Thang T Pham filed Critical Ciphergen Biosystems Inc
Priority to AU2002254298A priority Critical patent/AU2002254298A1/en
Publication of WO2002074927A2 publication Critical patent/WO2002074927A2/en
Publication of WO2002074927A3 publication Critical patent/WO2002074927A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The invention provides for the identification of target proteins in a sample based upon multiple sets of peptide fragment mass data obtained from the sample via gas phase ion spectroscopy. The sets of data are the product of analytical conditions that typically differ for each set such that cumulatively the data sets have higher information content than any individual set, thus enhancing the confidence level for accurate target protein identification. Probes, systems, and kits are additionally provided.
PCT/US2002/008450 2001-03-20 2002-03-18 High accuracy protein identification WO2002074927A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002254298A AU2002254298A1 (en) 2001-03-20 2002-03-18 High accuracy protein identification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27767701P 2001-03-20 2001-03-20
US60/277,677 2001-03-20

Publications (2)

Publication Number Publication Date
WO2002074927A2 WO2002074927A2 (en) 2002-09-26
WO2002074927A3 true WO2002074927A3 (en) 2003-02-27

Family

ID=23061918

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/008450 WO2002074927A2 (en) 2001-03-20 2002-03-18 High accuracy protein identification

Country Status (2)

Country Link
AU (1) AU2002254298A1 (en)
WO (1) WO2002074927A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012214202B4 (en) 2012-08-09 2016-05-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method for characterizing surface-adhesive properties of peptides and proteins
US10001456B2 (en) 2014-08-29 2018-06-19 Dh Technologies Development Pte. Ltd. Methods for detection in differential mobility spectrometer using a peptide metal complex
CN105067745B (en) * 2015-07-30 2017-01-25 广州供电局有限公司 Method for detecting dirty components through ion pairing based on fuzzy clustering
CN115112778B (en) * 2021-03-19 2023-08-04 复旦大学 Disease protein biomarker identification method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MANN ET AL.: "Analysis of proteins and proteomes by mass spectrometry", ANNU. REV. BIOCHEM., vol. 70, 2001, pages 437 - 473, XP002955539 *

Also Published As

Publication number Publication date
WO2002074927A2 (en) 2002-09-26
AU2002254298A1 (en) 2002-10-03

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