WO2002073209A3 - Verfahren zur identifikation von immunreaktiven epitopen auf proteinen - Google Patents

Verfahren zur identifikation von immunreaktiven epitopen auf proteinen Download PDF

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Publication number
WO2002073209A3
WO2002073209A3 PCT/EP2002/002629 EP0202629W WO02073209A3 WO 2002073209 A3 WO2002073209 A3 WO 2002073209A3 EP 0202629 W EP0202629 W EP 0202629W WO 02073209 A3 WO02073209 A3 WO 02073209A3
Authority
WO
WIPO (PCT)
Prior art keywords
immune
amino acids
proteins
peptides
diseases
Prior art date
Application number
PCT/EP2002/002629
Other languages
English (en)
French (fr)
Other versions
WO2002073209A2 (de
Inventor
Rudolf Kunze
Original Assignee
Affina Immuntechnik Gmbh
Rudolf Kunze
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affina Immuntechnik Gmbh, Rudolf Kunze filed Critical Affina Immuntechnik Gmbh
Priority to EP02729986A priority Critical patent/EP1410037A2/de
Priority to AU2002302410A priority patent/AU2002302410A1/en
Publication of WO2002073209A2 publication Critical patent/WO2002073209A2/de
Publication of WO2002073209A3 publication Critical patent/WO2002073209A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in eptitope analysis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Verfahren zur Identifikation von immunreaktiven Epitopen auf Proteinen von humanen Zellen, Geweben, Organen und von Mikrooganismen, die mit Erkrankungen wie Autoimmunerkrankungen, Krebserkrankungen oder Infektionen in Zusammenhang stehen, wobei Proben a) von Patienten mit diesen Erkrankungen aufgearbeitet werden, um Fraktionen zu erhalten, in denen Fa'b'-Antikörperfragmente und/oder Antigen erkennende Immunglobulinfragmente angereichert oder isoliert vorliegen, b) die im Schritt (a) angereicherten oder isolierten Fraktionen mit Peptiden, die mindestens zwölf Aminosäuren aufweisen und an einem Träger immobilisiert sind, in Kontakt gebracht werden, die durch Fragmentierung aus Proteinen oder Proteomen gewonnen werden, c) in einem Assay, bei dem Peptide mit mehr als zwölf Aminosäuren an Festphasen gebunden auf Wechselwirkung mit Fa'b'-Antikörperfragmenten und/oder Antigen erkennenden Immunglobulinfragmenten geprüft werden, woraufhin d) sich bei Wechselwirkungen zwischen den Fa'b'-Antikörperfragmenten und/oder Antigen erkennenden Immunglobulinfragmenten und dem mindestens zwölf Aminosäuren aufweisenden Peptid, das mindestens zwölf Aminosäuren aufweisende Peptid als immunreaktives Epitop zu erkennen gibt und ggf. weiter analysiert wird.
PCT/EP2002/002629 2001-03-10 2002-03-09 Verfahren zur identifikation von immunreaktiven epitopen auf proteinen WO2002073209A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02729986A EP1410037A2 (de) 2001-03-10 2002-03-09 Verfahren zur identifikation von immunreaktiven epitopen auf proteinen und verwendung der epitope zu prophylaktischen und therapeutischen zwecken
AU2002302410A AU2002302410A1 (en) 2001-03-10 2002-03-09 Method for identifying immune reactive epitopes on proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01105989.6 2001-03-10
EP01105989 2001-03-10

Publications (2)

Publication Number Publication Date
WO2002073209A2 WO2002073209A2 (de) 2002-09-19
WO2002073209A3 true WO2002073209A3 (de) 2003-03-13

Family

ID=8176743

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/002629 WO2002073209A2 (de) 2001-03-10 2002-03-09 Verfahren zur identifikation von immunreaktiven epitopen auf proteinen

Country Status (3)

Country Link
EP (1) EP1410037A2 (de)
AU (1) AU2002302410A1 (de)
WO (1) WO2002073209A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1859266A4 (de) * 2005-02-24 2010-07-28 Cemines Inc Zusammensetzungen und verfahren zur klassifizierung biologischer proben
US20070003954A1 (en) * 2005-05-12 2007-01-04 The Board Of Regents Of The University Of Texas System Protein and antibody profiling using small molecule microarrays
US9551721B2 (en) 2009-06-02 2017-01-24 The Board Of Regents Of The University Of Texas System Identification of small molecules recognized by antibodies in subjects with neurodegenerative diseases
WO2011047257A1 (en) 2009-10-16 2011-04-21 The Board Of Regents Of The University Of Texas System Compositions and methods for producing cyclic peptoid libraries

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565325A (en) * 1992-10-30 1996-10-15 Bristol-Myers Squibb Company Iterative methods for screening peptide libraries
WO2000029851A1 (en) * 1998-11-13 2000-05-25 Pepscan Systems B.V. A method for determining a mimotope sequence

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69919327D1 (de) * 1998-01-30 2009-11-26 Allertein Therapeutics Llc Prognostische allergie- oder entzündungstest

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565325A (en) * 1992-10-30 1996-10-15 Bristol-Myers Squibb Company Iterative methods for screening peptide libraries
WO2000029851A1 (en) * 1998-11-13 2000-05-25 Pepscan Systems B.V. A method for determining a mimotope sequence

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BLUETHNER M ET AL: "IDENTIFICATION OF MAJOR LINEAR EPITOPES ON THE SP100 NUCLEAR PBC AUTOANTIGEN BY THE GENE-FRAGMENT PHAGE-DISPLAY TECHNOLOGY", AUTOIMMUNITY, HARWOOD, CHUR, CH, vol. 29, no. 1, 1999, pages 33 - 42, XP001058410, ISSN: 0891-6934 *
GEVORKIAN G ET AL: "IMMUNODOMINANT SYNTHETIC PEPTIDES OF TAENIA CRASSICEPS IN MURINE AND HUMAN CYSTICERCOSIS", IMMUNOLOGY LETTERS, AMSTERDAM, NL, vol. 49, no. 3, 1996, pages 185 - 189, XP001058409, ISSN: 0165-2478 *
See also references of EP1410037A2 *
STEPHEN C W ET AL: "CHARACTERISATION OF EPITOPES ON HUMAN P53 USING PHAGE-DISPLAYED PEPTIDE LIBRARIES: INSIGHTS INTO ANTIBODY-PEPTIDE INTERACTIONS", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 248, 1995, pages 58 - 78, XP000619236, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
AU2002302410A1 (en) 2002-09-24
WO2002073209A2 (de) 2002-09-19
EP1410037A2 (de) 2004-04-21

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