WO2002070684A3 - Gene library for screening methods - Google Patents

Gene library for screening methods Download PDF

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Publication number
WO2002070684A3
WO2002070684A3 PCT/EP2002/000174 EP0200174W WO02070684A3 WO 2002070684 A3 WO2002070684 A3 WO 2002070684A3 EP 0200174 W EP0200174 W EP 0200174W WO 02070684 A3 WO02070684 A3 WO 02070684A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene library
screening methods
gene
relates
present
Prior art date
Application number
PCT/EP2002/000174
Other languages
French (fr)
Other versions
WO2002070684A2 (en
Inventor
Koegl Manfred
Original Assignee
Lion Bioscience Ag
Koegl Manfred
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lion Bioscience Ag, Koegl Manfred filed Critical Lion Bioscience Ag
Priority to AU2002249116A priority Critical patent/AU2002249116A1/en
Publication of WO2002070684A2 publication Critical patent/WO2002070684A2/en
Publication of WO2002070684A3 publication Critical patent/WO2002070684A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for generating a gene library characterised in that it contains substantially all genes of a desired source in substantially equimolar amounts, wherein each gene is represented by a sufficient amount of different fragments. The present invention also relates to a gene library obtainable by said method. The gene library of the invention is particularly useful for the 'yeast two hybrid' (Y2H) screening.
PCT/EP2002/000174 2001-01-11 2002-01-10 Gene library for screening methods WO2002070684A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002249116A AU2002249116A1 (en) 2001-01-11 2002-01-10 Gene library for screening methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01100654 2001-01-11
EP01100654.1 2001-01-11

Publications (2)

Publication Number Publication Date
WO2002070684A2 WO2002070684A2 (en) 2002-09-12
WO2002070684A3 true WO2002070684A3 (en) 2003-10-16

Family

ID=8176188

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/000174 WO2002070684A2 (en) 2001-01-11 2002-01-10 Gene library for screening methods

Country Status (2)

Country Link
AU (1) AU2002249116A1 (en)
WO (1) WO2002070684A2 (en)

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US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

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US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
ES2663234T3 (en) 2012-02-27 2018-04-11 Cellular Research, Inc Compositions and kits for molecular counting
ES2711168T3 (en) 2013-08-28 2019-04-30 Becton Dickinson Co Massive parallel analysis of individual cells
WO2015054292A1 (en) 2013-10-07 2015-04-16 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
EP3766988B1 (en) 2015-02-19 2024-02-14 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
ES2836802T3 (en) 2015-02-27 2021-06-28 Becton Dickinson Co Spatially addressable molecular barcodes
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US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
US10619186B2 (en) 2015-09-11 2020-04-14 Cellular Research, Inc. Methods and compositions for library normalization
EP4269616A3 (en) 2016-05-02 2024-02-14 Becton, Dickinson and Company Accurate molecular barcoding
US11397882B2 (en) 2016-05-26 2022-07-26 Becton, Dickinson And Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP4300099A3 (en) 2016-09-26 2024-03-27 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
CN117056774A (en) 2016-11-08 2023-11-14 贝克顿迪金森公司 Methods for cell marker classification
KR20190077062A (en) 2016-11-08 2019-07-02 셀룰러 리서치, 인크. How to classify expression profiles
CN110573253B (en) 2017-01-13 2021-11-02 赛卢拉研究公司 Hydrophilic coating for fluid channels
CN110382708A (en) 2017-02-01 2019-10-25 赛卢拉研究公司 Selective amplification is carried out using blocking property oligonucleotides
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11946095B2 (en) 2017-12-19 2024-04-02 Becton, Dickinson And Company Particles associated with oligonucleotides
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
CN112243461A (en) 2018-05-03 2021-01-19 贝克顿迪金森公司 Molecular barcoding at opposite transcript ends
CN112805389A (en) 2018-10-01 2021-05-14 贝克顿迪金森公司 Determination of 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins

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US5219989A (en) * 1988-12-13 1993-06-15 Mcgill University Bifunctional protein for the isolation of capped mRNA
US5637685A (en) * 1993-09-24 1997-06-10 The Trustees Of Columbia University In The City Of New York Normalized cDNA libraries
WO1998058085A1 (en) * 1997-06-16 1998-12-23 Diversa Corporation High throughput screening for novel enzymes
WO1999015702A1 (en) * 1997-09-24 1999-04-01 Life Technologies, Inc. Normalized nucleic acid libraries and methods of production thereof
WO1999067376A1 (en) * 1998-06-25 1999-12-29 Institut Pasteur Exhaustive analysis of viral protein interactions by two-hybrid screens and selection of correctly folded viral interacting polypeptides
WO2000046406A2 (en) * 1999-02-05 2000-08-10 Alphagene, Inc. Arrays for investigating protein protein interactions
WO2000053813A1 (en) * 1999-03-11 2000-09-14 Massachusetts Institute Of Technology Pangenomic libraries

Patent Citations (7)

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Publication number Priority date Publication date Assignee Title
US5219989A (en) * 1988-12-13 1993-06-15 Mcgill University Bifunctional protein for the isolation of capped mRNA
US5637685A (en) * 1993-09-24 1997-06-10 The Trustees Of Columbia University In The City Of New York Normalized cDNA libraries
WO1998058085A1 (en) * 1997-06-16 1998-12-23 Diversa Corporation High throughput screening for novel enzymes
WO1999015702A1 (en) * 1997-09-24 1999-04-01 Life Technologies, Inc. Normalized nucleic acid libraries and methods of production thereof
WO1999067376A1 (en) * 1998-06-25 1999-12-29 Institut Pasteur Exhaustive analysis of viral protein interactions by two-hybrid screens and selection of correctly folded viral interacting polypeptides
WO2000046406A2 (en) * 1999-02-05 2000-08-10 Alphagene, Inc. Arrays for investigating protein protein interactions
WO2000053813A1 (en) * 1999-03-11 2000-09-14 Massachusetts Institute Of Technology Pangenomic libraries

Non-Patent Citations (4)

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JAMES R HUDSON ET AL: "The complete set of predicted genes from Saccharomyces cerevisiae in a readily usable form", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 7, no. 12, December 1997 (1997-12-01), pages 1169 - 1173, XP002127444, ISSN: 1088-9051 *
KOHCHI TAKAYUKI ET AL: "Construction of an equalized cDNA library from Arabidopsis thaliana.", PLANT JOURNAL, vol. 8, no. 5, 1995, pages 771 - 776, XP002232644, ISSN: 0960-7412 *
PATANJALI S R ET AL: "CONSTRUCTION OF A UNIFORM-ABUNDANCE (NORMALIZED) CDNA LIBRARY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 88, no. 5, 1 March 1991 (1991-03-01), pages 1943 - 1947, XP000368687, ISSN: 0027-8424 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

Also Published As

Publication number Publication date
AU2002249116A1 (en) 2002-09-19
WO2002070684A2 (en) 2002-09-12

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