WO2002068697A3 - Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems - Google Patents

Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems Download PDF

Info

Publication number
WO2002068697A3
WO2002068697A3 PCT/US2002/006394 US0206394W WO02068697A3 WO 2002068697 A3 WO2002068697 A3 WO 2002068697A3 US 0206394 W US0206394 W US 0206394W WO 02068697 A3 WO02068697 A3 WO 02068697A3
Authority
WO
WIPO (PCT)
Prior art keywords
beads
methods
capture
dual bead
disc
Prior art date
Application number
PCT/US2002/006394
Other languages
French (fr)
Other versions
WO2002068697A2 (en
Inventor
Brigitte Chau Phan
Amethyst Hoang Lam
Ka-Yeun Yeung
Original Assignee
Burstein Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/997,741 external-priority patent/US20030003464A1/en
Priority claimed from US10/038,297 external-priority patent/US20030077598A1/en
Application filed by Burstein Technologies Inc filed Critical Burstein Technologies Inc
Priority to AU2002306636A priority Critical patent/AU2002306636A1/en
Publication of WO2002068697A2 publication Critical patent/WO2002068697A2/en
Publication of WO2002068697A3 publication Critical patent/WO2002068697A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods for decreasing non-specific bindings of beads in dual bead assays and related optical bio-discs and disc drive systems. The methods are employed to determine the suitability of a test solid phase for use in a dual bead assay. The methods include identifying whether a target agent is present in a biological sample and involve mixing capture beads, each having at least one transport probe affixed thereto. Reporter beads each have at least one signal probe affixed thereto. The reporter and capture beads are each bound to the target agent. The methods further include isolating the dual bead complex from the mixture to obtain an isolate, and exposing the isolate to a capture field on a disc. Detecting the presence of the dual bead complex in the disc is then performed to determine whether the target agent is present in the sample. The method further includes pre-treating capture beads and reporter beads with detergents prior to capture, treating capture beads and reporter beads with blocking agents prior to target capture, and performing the mixing in an intermittent manner. The beads are preferably mixed only when thay start to settle down in the tube or on the disc. The methods also provide for evaluation of non-specific binding of the dual bead assay in the presence of salt concentrations ranging from 0.1M up to 1M and use of a new wash buffer having 10mM EDTA.
PCT/US2002/006394 2001-02-28 2002-02-28 Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems WO2002068697A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002306636A AU2002306636A1 (en) 2001-02-28 2002-02-28 Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems

Applications Claiming Priority (20)

Application Number Priority Date Filing Date Title
US27213401P 2001-02-28 2001-02-28
US27224301P 2001-02-28 2001-02-28
US60/272,243 2001-02-28
US60/272,134 2001-02-28
US27252501P 2001-03-01 2001-03-01
US60/272,525 2001-03-01
US27500601P 2001-03-12 2001-03-12
US60/275,006 2001-03-12
US27564301P 2001-03-14 2001-03-14
US60/275,643 2001-03-14
US27869101P 2001-03-26 2001-03-26
US60/278,691 2001-03-26
US31490601P 2001-08-24 2001-08-24
US60/314,906 2001-08-24
US09/997,741 US20030003464A1 (en) 2000-11-27 2001-11-27 Dual bead assays including optical biodiscs and methods relating thereto
US09/997,741 2001-11-27
US10/038,297 US20030077598A1 (en) 2001-01-04 2002-01-04 Dual bead assays including covalent linkages for improved specificity and related optical analysis discs
US10/038,297 2002-01-04
US35227002P 2002-01-30 2002-01-30
US60/352,270 2002-01-30

Publications (2)

Publication Number Publication Date
WO2002068697A2 WO2002068697A2 (en) 2002-09-06
WO2002068697A3 true WO2002068697A3 (en) 2003-10-30

Family

ID=27580774

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/006394 WO2002068697A2 (en) 2001-02-28 2002-02-28 Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems

Country Status (2)

Country Link
AU (1) AU2002306636A1 (en)
WO (1) WO2002068697A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2691093A1 (en) * 2007-06-12 2008-12-18 Lingvitae Holding As Optical discs for analysing biomolecules
GB2474306A (en) * 2009-10-12 2011-04-13 Bioproducts Ltd Methods and device for detecting an analyte
CN112858701B (en) * 2019-11-28 2024-05-03 深圳迈瑞生物医疗电子股份有限公司 Mixing method of magnetic bead reagent and sample analyzer

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0504432A1 (en) * 1990-10-09 1992-09-23 Idemitsu Petrochemical Co. Ltd. Method of immunological quantitative analysis
WO1997021090A1 (en) * 1995-12-05 1997-06-12 Gamera Bioscience Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
WO1998038510A2 (en) * 1997-02-28 1998-09-03 Burstein Laboratories, Inc. Laboratory in a disk
WO1999035499A1 (en) * 1997-12-30 1999-07-15 Remacle Jose Method comprising capture molecule fixed on disc surface
WO2000005582A2 (en) * 1998-07-21 2000-02-03 Burstein Laboratories, Inc. Optical disc-based assay devices and methods
WO2000026677A1 (en) * 1998-10-30 2000-05-11 Burstein Laboratories, Inc. Trackable optical discs with concurrently readable analyte material
WO2002042498A2 (en) * 2000-11-27 2002-05-30 Burstein Technologies, Inc. Dual bead assays including optical biodiscs and methods relating thereto
WO2002068696A2 (en) * 2001-02-27 2002-09-06 Burstein Technologies, Inc. Methods for dna conjugation onto solid phase including related optical biodiscs and disc drive systems
WO2002071929A2 (en) * 2001-03-14 2002-09-19 Burnstein Technologies, Inc. Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0504432A1 (en) * 1990-10-09 1992-09-23 Idemitsu Petrochemical Co. Ltd. Method of immunological quantitative analysis
WO1997021090A1 (en) * 1995-12-05 1997-06-12 Gamera Bioscience Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
WO1998038510A2 (en) * 1997-02-28 1998-09-03 Burstein Laboratories, Inc. Laboratory in a disk
WO1999035499A1 (en) * 1997-12-30 1999-07-15 Remacle Jose Method comprising capture molecule fixed on disc surface
WO2000005582A2 (en) * 1998-07-21 2000-02-03 Burstein Laboratories, Inc. Optical disc-based assay devices and methods
WO2000026677A1 (en) * 1998-10-30 2000-05-11 Burstein Laboratories, Inc. Trackable optical discs with concurrently readable analyte material
WO2002042498A2 (en) * 2000-11-27 2002-05-30 Burstein Technologies, Inc. Dual bead assays including optical biodiscs and methods relating thereto
WO2002068696A2 (en) * 2001-02-27 2002-09-06 Burstein Technologies, Inc. Methods for dna conjugation onto solid phase including related optical biodiscs and disc drive systems
WO2002071929A2 (en) * 2001-03-14 2002-09-19 Burnstein Technologies, Inc. Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KIDO H ET AL: "DISC-BASED IMMUNOASSAY MICROARRAYS", ANALYTICA CHIMICA ACTA, ELSEVIER, AMSTERDAM, NL, vol. 411, no. 1/2, 2000, pages 1 - 11, XP001030179, ISSN: 0003-2670 *
SAMBROOK J ET AL: "MOLECULAR CLONING A LABORATORY MANUAL SECOND EDITION VOL. 1 + 2+ 3", MOLECULAR CLONING. A LABORATORY MANUAL, XX, XX, VOL. 2, PAGE(S) 9.47-9.51, XP002244240 *

Also Published As

Publication number Publication date
AU2002306636A1 (en) 2002-09-12
WO2002068697A2 (en) 2002-09-06

Similar Documents

Publication Publication Date Title
WO2002042498A3 (en) Dual bead assays including optical biodiscs and methods relating thereto
AU2014232882B2 (en) Automated immunoanalyzer system for performing diagnostic assays for allergies and autoimmune diseases
ATE161964T1 (en) BINDING TEST USING A LABELED REAGENT
ATE269541T1 (en) METHOD FOR PERFORMING TESTS
SE0000538L (en) Methods and kits for analyte detection using proximity probing
KR950019732A (en) Method of measuring urine protein and creatinine
KR970707299A (en) Compositions and Methods for the Simultaneous Detection and Quantification of Multiple Specific Nucleic Acid Sequences
ATE116443T1 (en) SOLID PHASE ANALYTICAL APPARATUS AND METHOD FOR USE THEREOF.
ATE431553T1 (en) METHOD FOR DETECTING ANALYTES USING MAGNETIC PARTICLES
WO2004019000A3 (en) Chemical reagents and methods for detection and quantification of proteins in complex mixtures
ATE297018T1 (en) HIGH-DENSITY LIPOPROTEINß TEST APPARATUS AND METHOD
JPS6447953A (en) Method and apparatus for separating labelled reagent for testing immunological bond measuring test
WO2002071929A3 (en) Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets
DE60325107D1 (en) METHOD AND KIT FOR THE QUANTITATIVE AND / OR QUALITATIVE DETECTION OF COMPONENTS IN A SAMPLE
ATE342506T1 (en) METHOD AND KIT FOR DETECTING AND IDENTIFYING CRYSTALS IN BIOLOGICAL LIQUIDS
CA1248433A (en) Process and reagent for the determination of fibrin monomer in plasma
ATE330225T1 (en) METHOD AND TEST KIT FOR DETECTING ANALYTES IN A SAMPLE
WO2002068697A3 (en) Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems
CA2390778A1 (en) Organ transplant rejection and associated conditions
ATE327514T1 (en) METHOD FOR QUANTIFYING CHOLESTEROL
ATE360206T1 (en) METHOD FOR MEASURING THE TOTAL CONCENTRATION OF AN ANALYTE
WO2002073605A3 (en) Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus
CN106771189A (en) A kind of fast qualitative quantitative detecting method of oil-adjuvant vaccine
DE50203136D1 (en) Method for detecting analytes in samples by means of analytical elements
DE60038557D1 (en) Assay

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP