WO2002068453A3 - Methods and compositions for the construction and use of fusion libraries using computational protein design methods - Google Patents

Methods and compositions for the construction and use of fusion libraries using computational protein design methods Download PDF

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Publication number
WO2002068453A3
WO2002068453A3 PCT/US2002/004853 US0204853W WO02068453A3 WO 2002068453 A3 WO2002068453 A3 WO 2002068453A3 US 0204853 W US0204853 W US 0204853W WO 02068453 A3 WO02068453 A3 WO 02068453A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
construction
protein design
computational protein
Prior art date
Application number
PCT/US2002/004853
Other languages
French (fr)
Other versions
WO2002068453A2 (en
Inventor
Min Li
Bassil I Dahiyat
Original Assignee
Xencor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xencor Inc filed Critical Xencor Inc
Priority to AU2002251999A priority Critical patent/AU2002251999A1/en
Publication of WO2002068453A2 publication Critical patent/WO2002068453A2/en
Publication of WO2002068453A3 publication Critical patent/WO2002068453A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

The invention relates to the use of protein design automation (PDA) to generate computationally prescreened secondary libraries of proteins, and to methods and compositions utilizing the libraries.
PCT/US2002/004853 2001-02-22 2002-02-19 Methods and compositions for the construction and use of fusion libraries using computational protein design methods WO2002068453A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002251999A AU2002251999A1 (en) 2001-02-22 2002-02-19 Methods and compositions for the construction and use of fusion libraries using computational protein design methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79262901A 2001-02-22 2001-02-22
US09/792,629 2001-02-22

Publications (2)

Publication Number Publication Date
WO2002068453A2 WO2002068453A2 (en) 2002-09-06
WO2002068453A3 true WO2002068453A3 (en) 2003-02-27

Family

ID=25157536

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/004853 WO2002068453A2 (en) 2001-02-22 2002-02-19 Methods and compositions for the construction and use of fusion libraries using computational protein design methods

Country Status (2)

Country Link
AU (1) AU2002251999A1 (en)
WO (1) WO2002068453A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8852586B2 (en) 2004-11-12 2014-10-07 Xencor, Inc. Fc variants with altered binding to FcRn
US8883147B2 (en) 2004-10-21 2014-11-11 Xencor, Inc. Immunoglobulins insertions, deletions, and substitutions
US8937158B2 (en) 2003-03-03 2015-01-20 Xencor, Inc. Fc variants with increased affinity for FcγRIIc
US9200079B2 (en) 2004-11-12 2015-12-01 Xencor, Inc. Fc variants with altered binding to FcRn

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9714282B2 (en) 2003-09-26 2017-07-25 Xencor, Inc. Optimized Fc variants and methods for their generation
US8399618B2 (en) 2004-10-21 2013-03-19 Xencor, Inc. Immunoglobulin insertions, deletions, and substitutions
CA2548817A1 (en) 2003-12-04 2005-06-23 Xencor, Inc. Methods of generating variant proteins with increased host string content and compositions thereof
CA2624189A1 (en) 2005-10-03 2007-04-12 Xencor, Inc. Fc variants with optimized fc receptor binding properties
WO2007136840A2 (en) * 2006-05-20 2007-11-29 Codon Devices, Inc. Nucleic acid library design and assembly
US20080287320A1 (en) * 2006-10-04 2008-11-20 Codon Devices Libraries and their design and assembly
LT2808343T (en) 2007-12-26 2019-09-10 Xencor Inc. Fc variants with altered binding to FcRn
WO2011028952A1 (en) 2009-09-02 2011-03-10 Xencor, Inc. Compositions and methods for simultaneous bivalent and monovalent co-engagement of antigens

Citations (6)

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Publication number Priority date Publication date Assignee Title
US5338665A (en) * 1991-10-16 1994-08-16 Affymax Technologies N.V. Peptide library and screening method
WO1998037186A1 (en) * 1997-02-18 1998-08-27 Actinova Limited In vitro peptide or protein expression library
US5856090A (en) * 1994-09-09 1999-01-05 The Scripps Research Institute DNA-methylase linking reaction
WO2000023564A2 (en) * 1998-10-16 2000-04-27 Xencor, Inc. Protein design automation for protein libraries
WO2001014539A2 (en) * 1999-08-20 2001-03-01 Johns Hopkins University School Of Medicine Methods and compositions for the construction and use of fusion libraries
WO2002022826A2 (en) * 2000-09-14 2002-03-21 Xencor, Inc. Methods and compositions for the construction and use of fusion libraries

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5338665A (en) * 1991-10-16 1994-08-16 Affymax Technologies N.V. Peptide library and screening method
US5856090A (en) * 1994-09-09 1999-01-05 The Scripps Research Institute DNA-methylase linking reaction
WO1998037186A1 (en) * 1997-02-18 1998-08-27 Actinova Limited In vitro peptide or protein expression library
WO2000023564A2 (en) * 1998-10-16 2000-04-27 Xencor, Inc. Protein design automation for protein libraries
WO2001014539A2 (en) * 1999-08-20 2001-03-01 Johns Hopkins University School Of Medicine Methods and compositions for the construction and use of fusion libraries
WO2002022826A2 (en) * 2000-09-14 2002-03-21 Xencor, Inc. Methods and compositions for the construction and use of fusion libraries

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DAHIYAT B I ET AL: "De novo protein design: fully automated sequence selection", SCIENCE, AAAS. LANCASTER, PA, US, vol. 278, no. 5335, 3 October 1997 (1997-10-03), pages 82 - 87, XP002188179, ISSN: 0036-8075 *
DAHIYAT B I ET AL: "De novo protein design: towards fully automated sequence selection.", JOURNAL OF MOLECULAR BIOLOGY. ENGLAND 7 NOV 1997, vol. 273, no. 4, 7 November 1997 (1997-11-07), pages 789 - 796, XP002208849, ISSN: 0022-2836 *
LI MIN: "Applications of display technology in protein analysis", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 18, no. 12, December 2000 (2000-12-01), pages 1251 - 1256, XP002162318, ISSN: 1087-0156 *
PRASAD K ET AL: "Characterization of the Rep78/AAV complex", VIROLOGY, ACADEMIC PRESS,ORLANDO, US, vol. 229, 1997, pages 183 - 192, XP002125033, ISSN: 0042-6822 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8937158B2 (en) 2003-03-03 2015-01-20 Xencor, Inc. Fc variants with increased affinity for FcγRIIc
US8883147B2 (en) 2004-10-21 2014-11-11 Xencor, Inc. Immunoglobulins insertions, deletions, and substitutions
US8852586B2 (en) 2004-11-12 2014-10-07 Xencor, Inc. Fc variants with altered binding to FcRn
US8883973B2 (en) 2004-11-12 2014-11-11 Xencor, Inc. Fc variants with altered binding to FcRn
US9200079B2 (en) 2004-11-12 2015-12-01 Xencor, Inc. Fc variants with altered binding to FcRn

Also Published As

Publication number Publication date
WO2002068453A2 (en) 2002-09-06
AU2002251999A1 (en) 2002-09-12

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