WO2002066656A3 - Detection of protein conformation using a split ubiquitin reporter system - Google Patents

Detection of protein conformation using a split ubiquitin reporter system Download PDF

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Publication number
WO2002066656A3
WO2002066656A3 PCT/US2002/000325 US0200325W WO02066656A3 WO 2002066656 A3 WO2002066656 A3 WO 2002066656A3 US 0200325 W US0200325 W US 0200325W WO 02066656 A3 WO02066656 A3 WO 02066656A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
reporter system
protein conformation
split ubiquitin
split
Prior art date
Application number
PCT/US2002/000325
Other languages
French (fr)
Other versions
WO2002066656A2 (en
Inventor
Nils Johnsson
Xavier Raquet
Alexander J Varshavsky
Jorg H Eckert
Original Assignee
Max Planck Gesellschaft
Nils Johnsson
Xavier Raquet
Alexander J Varshavsky
Jorg H Eckert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Nils Johnsson, Xavier Raquet, Alexander J Varshavsky, Jorg H Eckert filed Critical Max Planck Gesellschaft
Priority to US10/250,614 priority Critical patent/US20040053388A1/en
Priority to EP02718797A priority patent/EP1349943A2/en
Priority to CA002432489A priority patent/CA2432489A1/en
Publication of WO2002066656A2 publication Critical patent/WO2002066656A2/en
Publication of WO2002066656A3 publication Critical patent/WO2002066656A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention provides methods and reagents for monitoring protein structure by an intrapolypeptide split-ubiquitin assay.
PCT/US2002/000325 2001-01-04 2002-01-03 Detection of protein conformation using a split ubiquitin reporter system WO2002066656A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/250,614 US20040053388A1 (en) 2002-01-03 2002-01-03 Detection of protein conformation using a split ubiquitin reporter system
EP02718797A EP1349943A2 (en) 2001-01-04 2002-01-03 Detection of protein conformation using a split ubiquitin reporter system
CA002432489A CA2432489A1 (en) 2001-01-04 2002-01-03 Detection of protein conformation using a split ubiquitin reporter system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25982701P 2001-01-04 2001-01-04
US60/259,827 2001-01-04

Publications (2)

Publication Number Publication Date
WO2002066656A2 WO2002066656A2 (en) 2002-08-29
WO2002066656A3 true WO2002066656A3 (en) 2003-02-27

Family

ID=22986559

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000325 WO2002066656A2 (en) 2001-01-04 2002-01-03 Detection of protein conformation using a split ubiquitin reporter system

Country Status (3)

Country Link
EP (1) EP1349943A2 (en)
CA (1) CA2432489A1 (en)
WO (1) WO2002066656A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9169321B2 (en) 2007-08-22 2015-10-27 The Regents Of The University Of California Activatable binding polypeptides and methods of identification and use thereof

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7056683B2 (en) 2002-11-12 2006-06-06 Massachusetts Institute Of Technology Genetically encoded fluorescent reporters of kinase, methyltransferase, and acetyl-transferase activities
GB0404187D0 (en) * 2004-02-25 2004-03-31 Biotransformations Ltd Binding agents
EP2385955B1 (en) 2009-01-12 2020-08-12 CytomX Therapeutics, Inc. Modified antibody compositions, methods of making and using thereof
WO2010096838A2 (en) 2009-02-23 2010-08-26 Cytomx Therapeutics, Llc Proproteins and methods of use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JOHNSSON N ET AL: "SPLIT UBIQUITIN AS A SENSOR OF PROTEIN INTERACTIONS INV VIVO", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, 1 October 1994 (1994-10-01), pages 10340 - 10344, XP002064564, ISSN: 0027-8424 *
LASER H ET AL: "A NEW SCREEN FOR PROTEIN INTERACTIONS REVEALS THAT THE SACCHAROMYCES CEREVISIAE HIGH MOBILITY GROUP PROTEINS NHP6A/B ARE INVOLVED IN THE REGULATION OF THE GAL1 PROMOTER", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 25, no. 97, 5 December 2000 (2000-12-05), pages 13732 - 13737, XP001074698, ISSN: 0027-8424 *
WELLHAUSEN A ET AL: "Analysis of the in vivo interaction between a basic repressor and an acidic activator", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 453, no. 3, 25 June 1999 (1999-06-25), pages 299 - 304, XP004259892, ISSN: 0014-5793 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9169321B2 (en) 2007-08-22 2015-10-27 The Regents Of The University Of California Activatable binding polypeptides and methods of identification and use thereof

Also Published As

Publication number Publication date
CA2432489A1 (en) 2002-08-29
WO2002066656A2 (en) 2002-08-29
EP1349943A2 (en) 2003-10-08

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