WO2002064773A3 - Method for high throughput screening of candidate compounds by high throughput direct synthesis of recombinant polypeptides - Google Patents

Method for high throughput screening of candidate compounds by high throughput direct synthesis of recombinant polypeptides Download PDF

Info

Publication number
WO2002064773A3
WO2002064773A3 PCT/DE2002/000459 DE0200459W WO02064773A3 WO 2002064773 A3 WO2002064773 A3 WO 2002064773A3 DE 0200459 W DE0200459 W DE 0200459W WO 02064773 A3 WO02064773 A3 WO 02064773A3
Authority
WO
WIPO (PCT)
Prior art keywords
high throughput
candidate compounds
recombinant polypeptides
direct synthesis
throughput screening
Prior art date
Application number
PCT/DE2002/000459
Other languages
German (de)
French (fr)
Other versions
WO2002064773A2 (en
Inventor
Hans-Joachim Mueller
Original Assignee
Hans-Joachim Mueller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hans-Joachim Mueller filed Critical Hans-Joachim Mueller
Priority to AU2002247594A priority Critical patent/AU2002247594A1/en
Publication of WO2002064773A2 publication Critical patent/WO2002064773A2/en
Publication of WO2002064773A3 publication Critical patent/WO2002064773A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed is a method for high throughput direct synthesis of recombinant polypeptides which can be used directly for high throughput screening of candidate compounds after coupling to a supporting matrix.
PCT/DE2002/000459 2001-02-09 2002-02-07 Method for high throughput screening of candidate compounds by high throughput direct synthesis of recombinant polypeptides WO2002064773A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002247594A AU2002247594A1 (en) 2001-02-09 2002-02-07 Method for high throughput screening of candidate compounds by high throughput direct synthesis of recombinant polypeptides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10106339.3 2001-02-09
DE2001106339 DE10106339A1 (en) 2001-02-09 2001-02-09 Process for high throughput screening of candidate compounds via high throughput direct synthesis of recombinant polypeptides

Publications (2)

Publication Number Publication Date
WO2002064773A2 WO2002064773A2 (en) 2002-08-22
WO2002064773A3 true WO2002064773A3 (en) 2003-05-08

Family

ID=7673681

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/000459 WO2002064773A2 (en) 2001-02-09 2002-02-07 Method for high throughput screening of candidate compounds by high throughput direct synthesis of recombinant polypeptides

Country Status (3)

Country Link
AU (1) AU2002247594A1 (en)
DE (1) DE10106339A1 (en)
WO (1) WO2002064773A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000091A1 (en) * 1990-07-02 1992-01-09 Bioligand, Inc. Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
WO1992007949A1 (en) * 1990-11-05 1992-05-14 The United States Of America, As Represented By The Secretary Of The Army Method for the in vitro production of protein from a dna sequence without cloning
WO1994028028A1 (en) * 1993-05-27 1994-12-08 Selectide Corporation Topologically segregated, encoded solid phase libraries
WO1996014428A1 (en) * 1994-11-08 1996-05-17 Novagen, Inc. Method for in vitro protein synthesis
WO1998006739A1 (en) * 1996-08-16 1998-02-19 Clontech Laboratories, Inc. Method for purification of recombinant proteins
WO1998011249A1 (en) * 1996-09-13 1998-03-19 Garvin Alex M Mutation detection using peptide tagged in vitro synthesized proteins
WO1999012040A2 (en) * 1997-09-02 1999-03-11 Sequenom, Inc. Mass spectrometric detection of polypeptides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0542422A1 (en) * 1991-11-12 1993-05-19 General Atomics Multi-well microtiter plate
US5801233A (en) * 1992-10-02 1998-09-01 Arch Development Corporation Nucleic acid compositions encoding acetyl-coa carboxylase and uses therefor

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000091A1 (en) * 1990-07-02 1992-01-09 Bioligand, Inc. Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
WO1992007949A1 (en) * 1990-11-05 1992-05-14 The United States Of America, As Represented By The Secretary Of The Army Method for the in vitro production of protein from a dna sequence without cloning
WO1994028028A1 (en) * 1993-05-27 1994-12-08 Selectide Corporation Topologically segregated, encoded solid phase libraries
WO1996014428A1 (en) * 1994-11-08 1996-05-17 Novagen, Inc. Method for in vitro protein synthesis
WO1998006739A1 (en) * 1996-08-16 1998-02-19 Clontech Laboratories, Inc. Method for purification of recombinant proteins
WO1998011249A1 (en) * 1996-09-13 1998-03-19 Garvin Alex M Mutation detection using peptide tagged in vitro synthesized proteins
WO1999012040A2 (en) * 1997-09-02 1999-03-11 Sequenom, Inc. Mass spectrometric detection of polypeptides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LAM K S: "APPLICATION OF COMBINATORIAL LIBRARY METHODS IN CANCER RESEARCH AND DRUG DISCOVERY", ANTI-CANCER DRUG DESIGN, BASINGSTOKE, GB, vol. 12, April 1997 (1997-04-01), pages 145 - 167, XP000945295, ISSN: 0266-9536 *

Also Published As

Publication number Publication date
DE10106339A1 (en) 2002-08-22
AU2002247594A1 (en) 2002-08-28
WO2002064773A2 (en) 2002-08-22

Similar Documents

Publication Publication Date Title
GB0304576D0 (en) Protein a chromatography
EP1491560A4 (en) Process for the production of high-molecular compounds for photoresist
AU2003276537A1 (en) Process for conversion of biomass
IL166387A0 (en) Method for enhancing yield of recombinant protein production from plants
AU2002356755A1 (en) Process for production of detergent tablets
WO2001037147A3 (en) Apparatus and method for structure-based prediction of amino acid sequences
AU2002309998A1 (en) Electrochemical method for high-throughput screening of minute quantities of candidate compounds
WO2002064773A3 (en) Method for high throughput screening of candidate compounds by high throughput direct synthesis of recombinant polypeptides
AU2003298523A1 (en) Methods for efficient production of mammalian recombinant proteins
WO2003011849A8 (en) Process for the production of beraprost and its salts
AU2002340776A1 (en) High throughput isolation of proteins by charge induction chromatography
AU2002222658A1 (en) Novel aggrecanase
IL165527A0 (en) Use of fluorine NMR for high throughput screening
AU2003251750A1 (en) Method for cement clinker production using vitrified slag
AU2001295934A1 (en) Process for producing substituted aniline compound
WO2003084942A3 (en) Stereoselective alkylation of chiral 2-methyl-4-protected piperazines
AU2003226138A1 (en) Process for preparing phenylorganosilicon intermediates
HUP0500349A3 (en) Process for production of polypeptides
WO2002094949A3 (en) Detection method for identifying hydrolases
AU2003245554A1 (en) Process for screening fungitoxic compounds
AU2002367422A1 (en) Process for production of high-molecular compounds
AU2002242939A1 (en) Process for isolation of eupalitin (3-0-beta-d-galactopyranoside) from boerhavia diffusa
AU2003289958A8 (en) Process for the preparation of bicyclic hexa-peptide nepadutant
WO2004059000A3 (en) High-throughput screening method for identifying active substances by means of co-cultivation
HUP0203214A3 (en) Process for preparing intermediates applicable for synthesis of hiv protease inhibitor

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP