WO2002061145A3 - Amplification competitive de cibles fractionnees d'echantillons d'acides nucleiques multiples - Google Patents

Amplification competitive de cibles fractionnees d'echantillons d'acides nucleiques multiples Download PDF

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Publication number
WO2002061145A3
WO2002061145A3 PCT/US2002/003169 US0203169W WO02061145A3 WO 2002061145 A3 WO2002061145 A3 WO 2002061145A3 US 0203169 W US0203169 W US 0203169W WO 02061145 A3 WO02061145 A3 WO 02061145A3
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WO
WIPO (PCT)
Prior art keywords
sample
targets
nucleic acid
target
amplification
Prior art date
Application number
PCT/US2002/003169
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English (en)
Other versions
WO2002061145A2 (fr
Inventor
Matthew M Winkler
David Brown
Original Assignee
Ambion Inc
Matthew M Winkler
David Brown
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc, Matthew M Winkler, David Brown filed Critical Ambion Inc
Publication of WO2002061145A2 publication Critical patent/WO2002061145A2/fr
Publication of WO2002061145A3 publication Critical patent/WO2002061145A3/fr
Priority to US10/632,539 priority Critical patent/US20040110191A1/en
Priority to US10/632,534 priority patent/US20040058373A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés permettant de comparer une ou plusieurs cibles sur deux ou plus de deux populations d'acides nucléiques. On mélange les populations d'échantillons à comparer avant de fractionner le mélange d'échantillons en cibles, par hybridation avec des polynucléotides ou des oligonucléotides susceptibles d'être dissociés du mélange d'échantillons proprement dit. Ensuite, on amplifie la ou les cibles fractionnées pour que les cibles de chaque échantillon entrent en compétition vis-à-vis de réactifs d'amplification. Enfin, une quantification des produits d'amplification intervient pour différencier l'échantillon ayant engendré tel ou tel produit d'amplification. L'abondance relative de produits d'amplification issus de chaque population d'échantillons traduit le niveau de cible propre à chacun des échantillons originaux, donnant une comparaison directe du degré d'abondance des séquences cibles présentes dans les échantillons caractérisés.
PCT/US2002/003169 2001-01-31 2002-01-31 Amplification competitive de cibles fractionnees d'echantillons d'acides nucleiques multiples WO2002061145A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/632,539 US20040110191A1 (en) 2001-01-31 2003-07-31 Comparative analysis of nucleic acids using population tagging
US10/632,534 US20040058373A1 (en) 2001-01-31 2003-07-31 Competitive amplification of fractionated targets from multiple nucleic acid samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26569201P 2001-01-31 2001-01-31
US60/265,692 2001-01-31

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/632,534 Continuation US20040058373A1 (en) 2001-01-31 2003-07-31 Competitive amplification of fractionated targets from multiple nucleic acid samples
US10/632,539 Continuation US20040110191A1 (en) 2001-01-31 2003-07-31 Comparative analysis of nucleic acids using population tagging

Publications (2)

Publication Number Publication Date
WO2002061145A2 WO2002061145A2 (fr) 2002-08-08
WO2002061145A3 true WO2002061145A3 (fr) 2003-07-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/003169 WO2002061145A2 (fr) 2001-01-31 2002-01-31 Amplification competitive de cibles fractionnees d'echantillons d'acides nucleiques multiples

Country Status (1)

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WO (1) WO2002061145A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0870842A2 (fr) * 1997-04-07 1998-10-14 Japan Science and Technology Corporation PCR compétitive muni d'un adaptateur
WO2000024939A1 (fr) * 1998-10-27 2000-05-04 Affymetrix, Inc. Gestion de la complexite et analyse d'adn genomique

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0870842A2 (fr) * 1997-04-07 1998-10-14 Japan Science and Technology Corporation PCR compétitive muni d'un adaptateur
WO2000024939A1 (fr) * 1998-10-27 2000-05-04 Affymetrix, Inc. Gestion de la complexite et analyse d'adn genomique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAKAI KOHJI ET AL: "Microarray hybridization with fractionated cDNA: Enhanced identification of differentially expressed genes.", ANALYTICAL BIOCHEMISTRY, vol. 287, no. 1, 1 December 2000 (2000-12-01), pages 32 - 37, XP002225843, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
WO2002061145A2 (fr) 2002-08-08

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