WO2002059351A3 - Accessing microbial diversity by ecological methods - Google Patents

Accessing microbial diversity by ecological methods Download PDF

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Publication number
WO2002059351A3
WO2002059351A3 PCT/IS2002/000003 IS0200003W WO02059351A3 WO 2002059351 A3 WO2002059351 A3 WO 2002059351A3 IS 0200003 W IS0200003 W IS 0200003W WO 02059351 A3 WO02059351 A3 WO 02059351A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
microbial diversity
ecological methods
accessing
microbial population
Prior art date
Application number
PCT/IS2002/000003
Other languages
French (fr)
Other versions
WO2002059351A2 (en
Inventor
Viggo T Marteinsson
Cedric Hobel
Olafur H Fridjonsson
Gudmundur Oli Hreggvidsson
Jakob K Kristjansson
Original Assignee
Prokaria Ehf
Viggo T Marteinsson
Cedric Hobel
Olafur H Fridjonsson
Gudmundur Oli Hreggvidsson
Jakob K Kristjansson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ehf, Viggo T Marteinsson, Cedric Hobel, Olafur H Fridjonsson, Gudmundur Oli Hreggvidsson, Jakob K Kristjansson filed Critical Prokaria Ehf
Priority to AU2002225323A priority Critical patent/AU2002225323A1/en
Publication of WO2002059351A2 publication Critical patent/WO2002059351A2/en
Publication of WO2002059351A3 publication Critical patent/WO2002059351A3/en
Priority to IS6884A priority patent/IS6884A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics

Abstract

Methods for enriching a microbial population from a natural environment, such as an oligotrophic environment (e.g., aquatic, marine, extreme or geothermal) are described. Also described are methods for screening for bioactive small molecules in an enriched microbial population. Previously uncultivated microorganisms can be cultured, isolated and their nucleic acids identified by methods described herein. Methods also describe phylogenetic characterization of enriched microbial populations.
PCT/IS2002/000003 2001-01-26 2002-01-25 Accessing microbial diversity by ecological methods WO2002059351A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002225323A AU2002225323A1 (en) 2001-01-26 2002-01-25 Accessing microbial diversity by ecological methods
IS6884A IS6884A (en) 2001-01-26 2003-07-24 Methods of utilizing biodiversity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77077101A 2001-01-26 2001-01-26
US09/770,771 2001-01-26

Publications (2)

Publication Number Publication Date
WO2002059351A2 WO2002059351A2 (en) 2002-08-01
WO2002059351A3 true WO2002059351A3 (en) 2003-02-13

Family

ID=25089626

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IS2002/000003 WO2002059351A2 (en) 2001-01-26 2002-01-25 Accessing microbial diversity by ecological methods

Country Status (3)

Country Link
AU (1) AU2002225323A1 (en)
IS (1) IS6884A (en)
WO (1) WO2002059351A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2923491B1 (en) 2007-11-14 2012-12-14 Deinove Sa USE OF BACTERIA FOR THE PRODUCTION OF BIOENERGY SOURCES
EP2210935A1 (en) 2009-01-19 2010-07-28 Deinove Methods for isolating bacteria
EP2218773A1 (en) 2009-02-17 2010-08-18 Deinove Compositions and methods for degrading lignocellulosic biomass
EP2251415A1 (en) 2009-05-14 2010-11-17 Deinove Recombinant bacteria and the uses thereof for producing ethanol
EP2251414A1 (en) 2009-05-14 2010-11-17 Deinove High performance metabolic bacteria
CN102884175A (en) 2010-03-02 2013-01-16 德诺芙公司 Bacteria and the uses thereof
WO2017209990A1 (en) 2016-05-31 2017-12-07 Exxonmobil Upstream Research Company METHODS FOR lSOLATING NUCLEIC ACIDS FROM SAMPLES
US10895666B2 (en) 2016-07-01 2021-01-19 Exxonmobil Upstream Research Company Methods for identifying hydrocarbon reservoirs
US10132144B2 (en) 2016-09-02 2018-11-20 Exxonmobil Upstream Research Company Geochemical methods for monitoring and evaluating microbial enhanced recovery operations
CN114350508B (en) * 2022-03-17 2022-07-12 南方海洋科学与工程广东省实验室(广州) High-pressure environment marine microorganism enrichment culture and gravity type separation device

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3892130A (en) * 1974-02-11 1975-07-01 Us Navy Deep sea microbiological sampling and culturing apparatus and method
US5559295A (en) * 1994-12-01 1996-09-24 Sheryll; Richard P. Underwater sampling method and apparatus
JPH09311727A (en) * 1996-05-22 1997-12-02 Mitsubishi Heavy Ind Ltd Method and unit for heating and controlling pressurized tank
WO1999010472A1 (en) * 1997-08-26 1999-03-04 Diversa Corporation Coated surfaces for selective enrichment of microbial populations
US6001574A (en) * 1996-06-18 1999-12-14 Diversa Corporation Production and use of normalized DNA libraries
US20010041333A1 (en) * 1997-06-16 2001-11-15 Short Jay M. High throughput screening for a bioactivity or biomolecule

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3892130A (en) * 1974-02-11 1975-07-01 Us Navy Deep sea microbiological sampling and culturing apparatus and method
US5559295A (en) * 1994-12-01 1996-09-24 Sheryll; Richard P. Underwater sampling method and apparatus
JPH09311727A (en) * 1996-05-22 1997-12-02 Mitsubishi Heavy Ind Ltd Method and unit for heating and controlling pressurized tank
US6001574A (en) * 1996-06-18 1999-12-14 Diversa Corporation Production and use of normalized DNA libraries
US20010041333A1 (en) * 1997-06-16 2001-11-15 Short Jay M. High throughput screening for a bioactivity or biomolecule
WO1999010472A1 (en) * 1997-08-26 1999-03-04 Diversa Corporation Coated surfaces for selective enrichment of microbial populations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199807, Derwent World Patents Index; Class J04, AN 1998-073530, XP002902563 *
GROSSKOPF R ET AL: "Diversity and structure of the methanogenic community in anoxic rice paddy soil microcosms as examined by cultivation and direct 16S rRNA gene sequence retrieval.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 3, March 1998 (1998-03-01), pages 960 - 969, XP002207615 *
HUBER R ET AL: "Thermocrinis ruber gen. nov., sp. nov., a pink-filament-forming hyperthermophilic bacterium isolated from yellowstone national park.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 10, October 1998 (1998-10-01), pages 3576 - 3583, XP002902564 *

Also Published As

Publication number Publication date
WO2002059351A2 (en) 2002-08-01
AU2002225323A1 (en) 2002-08-06
IS6884A (en) 2003-07-24

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