WO2002057482A3 - Method for differential analysis of bacteria in a sample - Google Patents

Method for differential analysis of bacteria in a sample Download PDF

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Publication number
WO2002057482A3
WO2002057482A3 PCT/DK2002/000036 DK0200036W WO02057482A3 WO 2002057482 A3 WO2002057482 A3 WO 2002057482A3 DK 0200036 W DK0200036 W DK 0200036W WO 02057482 A3 WO02057482 A3 WO 02057482A3
Authority
WO
WIPO (PCT)
Prior art keywords
gram
bacteria
sample
positive
negative bacteria
Prior art date
Application number
PCT/DK2002/000036
Other languages
French (fr)
Other versions
WO2002057482A2 (en
Inventor
Lene Jespersen
Claus Holm
Original Assignee
Royal Veterinary & Agricultural Univ
Danish Dairy Board
Lene Jespersen
Claus Holm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Royal Veterinary & Agricultural Univ, Danish Dairy Board, Lene Jespersen, Claus Holm filed Critical Royal Veterinary & Agricultural Univ
Priority to AU2002224757A priority Critical patent/AU2002224757A1/en
Publication of WO2002057482A2 publication Critical patent/WO2002057482A2/en
Publication of WO2002057482A3 publication Critical patent/WO2002057482A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

A method for analysing a bacterial smaple, such as a milk sample, by fluorescence determination, in particular by flow cytometry. In particular, there is provided a method for the determination of the proportion of Gram-positive and Gram-negative bacteria, respectively, in a one step procedure in a sample containing said bacteria in an unfixed state, by contacting the sample wiht a detectable moiety capable of selectively binding to either Gram-positive or Gram-negative bacteria, and a detectable moiety capable of binding to both Gram-positive and Gram-negative bacteria. The method is useful in e.g. the dairy industry for a rapid determination of the total number of bacteria and to distinguish between Gram-positive and Gram-negative bacteria in raw, pasteurised or processed milk.
PCT/DK2002/000036 2001-01-17 2002-01-17 Method for differential analysis of bacteria in a sample WO2002057482A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002224757A AU2002224757A1 (en) 2001-01-17 2002-01-17 Method for differential analysis of bacteria in a sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200100085 2001-01-17
DKPA200100085 2001-01-17

Publications (2)

Publication Number Publication Date
WO2002057482A2 WO2002057482A2 (en) 2002-07-25
WO2002057482A3 true WO2002057482A3 (en) 2003-06-05

Family

ID=8160043

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000036 WO2002057482A2 (en) 2001-01-17 2002-01-17 Method for differential analysis of bacteria in a sample

Country Status (2)

Country Link
AU (1) AU2002224757A1 (en)
WO (1) WO2002057482A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013134689A1 (en) * 2012-03-09 2013-09-12 Environmental Technology Solutions Llc A biochemical sensor for quantitative simultaneous multi-species bacteria detection in situ
FR3082523B1 (en) 2018-06-18 2022-11-18 Maat Pharma METHOD FOR DETECTING BACTERIA ACCORDING TO THEIR GRAM SIGNAL IN A COMPLEX SAMPLE
CN112945914A (en) * 2019-12-10 2021-06-11 中国科学院大连化学物理研究所 Fluorescence detection method for rapidly distinguishing gram types of bacteria
CN113403364A (en) * 2019-12-16 2021-09-17 中国计量科学研究院 Rapid quantitative detection method and kit for total number of bacteria in cow milk
CN112575054B (en) * 2019-12-16 2022-12-16 中国计量科学研究院 Rapid synchronous multiple detection method and kit for total number of listeria monocytogenes and bacteria
CN112553292A (en) * 2019-12-16 2021-03-26 中国计量科学研究院 Rapid synchronous multiple detection method and kit for total number of salmonella and bacteria

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2031457A (en) * 1978-09-25 1980-04-23 Baylor College Medicine Staining and analysis of bacteria
US4665024A (en) * 1984-10-01 1987-05-12 Becton, Dickinson And Company Fluorescent gram stain
EP0494085A1 (en) * 1985-09-27 1992-07-08 The Regents Of The University Of California Monoclonal antibodies binding determinants of gram negative bacteria

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2031457A (en) * 1978-09-25 1980-04-23 Baylor College Medicine Staining and analysis of bacteria
US4665024A (en) * 1984-10-01 1987-05-12 Becton, Dickinson And Company Fluorescent gram stain
EP0494085A1 (en) * 1985-09-27 1992-07-08 The Regents Of The University Of California Monoclonal antibodies binding determinants of gram negative bacteria

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1985, RODRIGUES U M ET AL: "THE DIRECT EPIFLUORESCENT FILTER TECHNIQUE INCREASED SELECTIVITY SENSITIVITY AND RAPIDITY", XP002182342, Database accession no. PREV198681091449 *
DOMMETT T W ET AL: "Estimation of residual shelf life of pasteurised milk using the PCA-ANS method.", AUSTRALIAN JOURNAL OF DAIRY TECHNOLOGY, vol. 49, no. 1, 1994, pages 48 - 53, XP002182340, ISSN: 0004-9433 *
JOURNAL OF APPLIED BACTERIOLOGY, vol. 59, no. 6, 1985, pages 493 - 500, ISSN: 0021-8847 *
KWEE W S ET AL: "FLUOROGENIC MEDIUM FOR DIFFERENTIAL GRAM-NEGATIVE GRAM-POSITIVE AND TOTAL BACTERIAL COUNTS IN LIQUID MILKS", AUSTRALIAN JOURNAL OF DAIRY TECHNOLOGY, vol. 43, no. 2, 1988, pages 50 - 53, XP002182341, ISSN: 0004-9433 *

Also Published As

Publication number Publication date
WO2002057482A2 (en) 2002-07-25
AU2002224757A1 (en) 2002-07-30

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