WO2002056004A3 - Thin film electrophoresis apparatus and method - Google Patents

Thin film electrophoresis apparatus and method Download PDF

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Publication number
WO2002056004A3
WO2002056004A3 PCT/US2002/000985 US0200985W WO02056004A3 WO 2002056004 A3 WO2002056004 A3 WO 2002056004A3 US 0200985 W US0200985 W US 0200985W WO 02056004 A3 WO02056004 A3 WO 02056004A3
Authority
WO
WIPO (PCT)
Prior art keywords
data
thin film
electrophoresis apparatus
electrophoresis
film electrophoresis
Prior art date
Application number
PCT/US2002/000985
Other languages
French (fr)
Other versions
WO2002056004A2 (en
WO2002056004A8 (en
Inventor
Andras Guttman
Bart Wanders
Phil Alei
Original Assignee
Syngenta Participations Ag
Andras Guttman
Bart Wanders
Phil Alei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, Andras Guttman, Bart Wanders, Phil Alei filed Critical Syngenta Participations Ag
Priority to EP02705777A priority Critical patent/EP1412733A2/en
Priority to CA002434469A priority patent/CA2434469A1/en
Priority claimed from US10/047,461 external-priority patent/US20030132117A1/en
Publication of WO2002056004A2 publication Critical patent/WO2002056004A2/en
Publication of WO2002056004A8 publication Critical patent/WO2002056004A8/en
Publication of WO2002056004A3 publication Critical patent/WO2002056004A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Electrochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

High throughput electrophoresis having a plurality of samples migrating through a highly resistive separatory media under an electric field. Multiple electrophoresis runs are conducted using a single separatory media. Automated methods of data analysis yields quantitative and qualitative data using optical densitometer or other detection systems. Controlled operating temperatures and small geometry reduces dispersion inaccuracy in banded data.
PCT/US2002/000985 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method WO2002056004A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02705777A EP1412733A2 (en) 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method
CA002434469A CA2434469A1 (en) 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US75998901A 2001-01-12 2001-01-12
US09/759,989 2001-01-12
US10/047,461 2002-01-14
US10/047,461 US20030132117A1 (en) 2002-01-14 2002-01-14 Thin film electrophoresis apparatus and method

Publications (3)

Publication Number Publication Date
WO2002056004A2 WO2002056004A2 (en) 2002-07-18
WO2002056004A8 WO2002056004A8 (en) 2003-01-16
WO2002056004A3 true WO2002056004A3 (en) 2004-02-19

Family

ID=26725040

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2002/000985 WO2002056004A2 (en) 2001-01-12 2002-01-14 Thin film electrophoresis apparatus and method
PCT/US2002/000993 WO2002055189A2 (en) 2001-01-12 2002-01-14 Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000993 WO2002055189A2 (en) 2001-01-12 2002-01-14 Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics

Country Status (3)

Country Link
AU (1) AU2002239908A1 (en)
CA (1) CA2434469A1 (en)
WO (2) WO2002056004A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2422908B (en) * 2003-09-22 2007-11-21 Shimadzu Corp Electrophoretic member electrophoretic device electrophoretic method and sample dispensing probe
GB2439233B (en) * 2003-09-22 2008-04-23 Shimadzu Corp An electrophoretic separation method
CN100447567C (en) * 2004-09-22 2008-12-31 杭州生源医疗保健技术开发有限公司 Composite ionic membrane micro electric chromatography
US8206974B2 (en) 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
ES2674101T3 (en) 2007-04-04 2018-06-27 Ande Corporation Plastic microfluidic separation and detection platforms
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
EP2443254A2 (en) 2009-06-15 2012-04-25 NetBio, Inc. Improved methods for forensic dna quantitation
CA2841752A1 (en) 2011-07-14 2013-06-13 The George Washington University Plume collimation for laser ablation electrospray ionization mass spectrometry
GB2502953B (en) * 2012-05-24 2017-02-08 Imp Innovations Ltd Catalytic converter substrate
NL2009191C2 (en) * 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB2517951A (en) 2013-09-05 2015-03-11 Imp Innovations Ltd A substrate and a method of manufacturing a substrate
US10115577B1 (en) * 2017-09-07 2018-10-30 California Institute Of Technology Isotope ratio mass spectrometry

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2560220A1 (en) * 1984-02-27 1985-08-30 Int Biotechnologies In HORIZONTAL GEL ELECTROPHORESIS DEVICE
EP0492769A2 (en) * 1990-12-20 1992-07-01 Wisconsin Alumni Research Foundation Horizontal gel electrophoresis apparatus
US5338426A (en) * 1992-08-28 1994-08-16 Applied Biosystems, Inc. Horizontal polyacrylamide gel electrophoresis
EP0911631A1 (en) * 1991-01-04 1999-04-28 Sebia Apparatus for the application of biological samples to an electrophoresis plate
WO2000065336A1 (en) * 1999-04-26 2000-11-02 Mj Research, Inc. Electrophoresis assembly and method of casting electrophoresis gels
WO2000068677A1 (en) * 1999-05-06 2000-11-16 University Of Southampton Gel electrophoresis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5990373A (en) * 1996-08-20 1999-11-23 Kansas State University Research Foundation Nanometer sized metal oxide particles for ambient temperature adsorption of toxic chemicals
US6057488A (en) * 1998-09-15 2000-05-02 Nantek, Inc. Nanoparticles for the destructive sorption of biological and chemical contaminants

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2560220A1 (en) * 1984-02-27 1985-08-30 Int Biotechnologies In HORIZONTAL GEL ELECTROPHORESIS DEVICE
EP0492769A2 (en) * 1990-12-20 1992-07-01 Wisconsin Alumni Research Foundation Horizontal gel electrophoresis apparatus
EP0911631A1 (en) * 1991-01-04 1999-04-28 Sebia Apparatus for the application of biological samples to an electrophoresis plate
US5338426A (en) * 1992-08-28 1994-08-16 Applied Biosystems, Inc. Horizontal polyacrylamide gel electrophoresis
WO2000065336A1 (en) * 1999-04-26 2000-11-02 Mj Research, Inc. Electrophoresis assembly and method of casting electrophoresis gels
WO2000068677A1 (en) * 1999-05-06 2000-11-16 University Of Southampton Gel electrophoresis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ERFLE H ET AL: "SIMULTANEOUS LOADING OF 200 SAMPLE LANES FOR DNA SEQUENCING ON VERTICAL AND HORIZONTAL, STANDARD AND ULTRATHIN GELS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 11, 1997, pages 2229 - 2230, XP000858128, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2002055189A2 (en) 2002-07-18
WO2002056004A2 (en) 2002-07-18
WO2002056004A8 (en) 2003-01-16
AU2002239908A1 (en) 2002-07-24
WO2002055189A3 (en) 2004-10-28
CA2434469A1 (en) 2002-07-18

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