WO2002055188A3 - System and process for microfluidics-based automated chemistry - Google Patents

System and process for microfluidics-based automated chemistry Download PDF

Info

Publication number
WO2002055188A3
WO2002055188A3 PCT/US2002/000370 US0200370W WO02055188A3 WO 2002055188 A3 WO2002055188 A3 WO 2002055188A3 US 0200370 W US0200370 W US 0200370W WO 02055188 A3 WO02055188 A3 WO 02055188A3
Authority
WO
WIPO (PCT)
Prior art keywords
rotary
valves
computer
small
control
Prior art date
Application number
PCT/US2002/000370
Other languages
French (fr)
Other versions
WO2002055188A9 (en
WO2002055188A2 (en
Inventor
Michael Powell
Paul Tempst
Original Assignee
Sloan Kettering Inst Cancer
Michael Powell
Paul Tempst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sloan Kettering Inst Cancer, Michael Powell, Paul Tempst filed Critical Sloan Kettering Inst Cancer
Priority to AU2002241818A priority Critical patent/AU2002241818A1/en
Priority to US10/203,235 priority patent/US6813568B2/en
Publication of WO2002055188A2 publication Critical patent/WO2002055188A2/en
Publication of WO2002055188A9 publication Critical patent/WO2002055188A9/en
Publication of WO2002055188A3 publication Critical patent/WO2002055188A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/122—Hydrolysis with acids different from HF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K11/00—Multiple-way valves, e.g. mixing valves; Pipe fittings incorporating such valves
    • F16K11/02—Multiple-way valves, e.g. mixing valves; Pipe fittings incorporating such valves with all movable sealing faces moving as one unit
    • F16K11/06—Multiple-way valves, e.g. mixing valves; Pipe fittings incorporating such valves with all movable sealing faces moving as one unit comprising only sliding valves, i.e. sliding closure elements
    • F16K11/072—Multiple-way valves, e.g. mixing valves; Pipe fittings incorporating such valves with all movable sealing faces moving as one unit comprising only sliding valves, i.e. sliding closure elements with pivoted closure members
    • F16K11/074—Multiple-way valves, e.g. mixing valves; Pipe fittings incorporating such valves with all movable sealing faces moving as one unit comprising only sliding valves, i.e. sliding closure elements with pivoted closure members with flat sealing faces
    • F16K11/0743—Multiple-way valves, e.g. mixing valves; Pipe fittings incorporating such valves with all movable sealing faces moving as one unit comprising only sliding valves, i.e. sliding closure elements with pivoted closure members with flat sealing faces with both the supply and the discharge passages being on one side of the closure plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/00391—Rotary valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/00391—Rotary valves
    • B01J2219/00394—Rotary valves in multiple arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Mechanical Engineering (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention is a chemical system and related processes that utilize small-volume rotary selector valves and small-volume rotary switching valves in combination under the control of a computer. The small rotary valves are partivular well-suited to computer control. As a result, a single system can be programmed to do a variety of tasks by changing the program but leaving the system's components substantially intact. As a result, in a general aspect the invention is a system for carrying out one or more chemical reactions such as protein sequencing, said system comprising a rotary selector valve and a rotary switching valve, each valve under the control of a computer, the internal volumes of the selector and switching valves each being 1.5 ul o5 less.
PCT/US2002/000370 2001-01-10 2002-01-09 System and process for microfluidics-based automated chemistry WO2002055188A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002241818A AU2002241818A1 (en) 2001-01-10 2002-01-09 System and process for microfluidics-based automated chemistry
US10/203,235 US6813568B2 (en) 2002-01-09 2002-01-09 System and process for microfluidics-based automated chemistry

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26072601P 2001-01-10 2001-01-10
US60/260,726 2001-01-10

Publications (3)

Publication Number Publication Date
WO2002055188A2 WO2002055188A2 (en) 2002-07-18
WO2002055188A9 WO2002055188A9 (en) 2002-10-31
WO2002055188A3 true WO2002055188A3 (en) 2003-04-17

Family

ID=22990354

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000370 WO2002055188A2 (en) 2001-01-10 2002-01-09 System and process for microfluidics-based automated chemistry

Country Status (2)

Country Link
AU (1) AU2002241818A1 (en)
WO (1) WO2002055188A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7195026B2 (en) * 2002-12-27 2007-03-27 American Air Liquide, Inc. Micro electromechanical systems for delivering high purity fluids in a chemical delivery system
EP2537657A3 (en) 2005-08-09 2016-05-04 The University of North Carolina At Chapel Hill Methods and materials for fabricating microfluidic devices
US8298763B2 (en) * 2007-03-02 2012-10-30 Lawrence Livermore National Security, Llc Automated high-throughput flow-through real-time diagnostic system
CN102236023B (en) * 2011-03-30 2013-01-09 深圳市麦迪聪医疗电子有限公司 Flowing type multi-channel biochemical analyzer

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541114A (en) * 1992-07-23 1996-07-30 City Of Hope Sequencing of protein immobilized on polytetrafluoroethylene supports
JPH0968486A (en) * 1995-08-31 1997-03-11 Toyota Central Res & Dev Lab Inc Time sharing high speed sampler, automatic analyzer and analyzing method
US5695720A (en) * 1995-04-03 1997-12-09 B.C. Research Inc. Flow analysis network apparatus
WO2000064557A1 (en) * 1999-04-23 2000-11-02 Advanced Bioanalytical Services, Inc. High-throughput parallel liquid chromatography system
FR2809490A1 (en) * 2000-05-23 2001-11-30 Hocer INSTALLATION AND METHOD FOR THE AUTOMATIC PREPARATION OF SAMPLES

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541114A (en) * 1992-07-23 1996-07-30 City Of Hope Sequencing of protein immobilized on polytetrafluoroethylene supports
US5695720A (en) * 1995-04-03 1997-12-09 B.C. Research Inc. Flow analysis network apparatus
JPH0968486A (en) * 1995-08-31 1997-03-11 Toyota Central Res & Dev Lab Inc Time sharing high speed sampler, automatic analyzer and analyzing method
WO2000064557A1 (en) * 1999-04-23 2000-11-02 Advanced Bioanalytical Services, Inc. High-throughput parallel liquid chromatography system
FR2809490A1 (en) * 2000-05-23 2001-11-30 Hocer INSTALLATION AND METHOD FOR THE AUTOMATIC PREPARATION OF SAMPLES

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DAIXIN TONG ET AL.: "Principles and applications of unified chromatography", JOURNAL OF CHROMATOGRAPHY A, vol. 703, no. 1, 26 May 1995 (1995-05-26), NL, pages 17 - 35, XP004023358, ISSN: 0021-9673 *
DATABASE WPI Section Ch Week 199720, Derwent World Patents Index; Class J04, AN 1997-222795, XP002228887 *
HANS G.J. MOL ET AL.: "Trace level analysis of micropollutants in aqueous samples using gas chromatography with on-line sample enrichment and large volume injection", JOURNAL OF CHROMATOGRAPHY A, vol. 703, no. 1, 26 May 1995 (1995-05-26), NL, pages 277 - 307, XP004023368, ISSN: 0021-9673 *
MICHAEL POWELL & PAUL TEMPST: "Microflow-Based Automated Chemistries: Application to Protein Sequencing", ANALYTICAL CHEMISTRY, vol. 73, no. 4, 19 January 2001 (2001-01-19), AMERICAN CHEMICAL SOCIETY. COLUMBUS., US, pages 776 - 786, XP001092853, ISSN: 0003-2700 *
PATENT ABSTRACTS OF JAPAN vol. 1997, no. 07 31 July 1997 (1997-07-31) *

Also Published As

Publication number Publication date
AU2002241818A1 (en) 2002-07-24
WO2002055188A9 (en) 2002-10-31
WO2002055188A2 (en) 2002-07-18

Similar Documents

Publication Publication Date Title
DE69917527D1 (en) Flow control valve, especially for fumigated liquids
WO2000051720A3 (en) Chemical processing microsystems and methods for preparing and using same
WO2000062051A3 (en) Methods and compositions for conducting processes in microfluidic devices
EP1454085A4 (en) Process control valve
DE59804229D1 (en) Valve for controlling liquids
MXPA03007157A (en) Improved mixture concentration control in manufacturing processes.
BR9609792A (en) Dna gene epsps protein mutated chimeric vector gene for plant transformation plant cell plant method for plant production and plant treatment method
WO2002094864A3 (en) Human cdnas and proteins and uses thereof
WO2002055188A3 (en) System and process for microfluidics-based automated chemistry
WO2003045862A3 (en) Method for the production of borosilicate glass with a surface suitable for modification, glass obtained according to said method and the use thereof
WO2004046685A3 (en) Quality control of assays
AU2002349344A1 (en) Microfluidic extraction method
WO2006021584A3 (en) Purification of factor xiii polypeptides from biological materials
WO2002042475A8 (en) Gene hd3a inducing flowering of plant and utilization thereof
PL322885A1 (en) Flow control ports for controlling inflow into a thermostatic mixing valve
EP1239351A3 (en) Methods and computer program products for automated experimental design
DE59809801D1 (en) Valve for controlling liquids
WO1999043791A3 (en) Diisopropyl fluorophosphatase and the utilization and production thereof
WO2004035734A3 (en) Plastid transformation
WO2003014350A1 (en) Novel rice gene regulating salt stress tolerance
FR2839660B1 (en) MICROREACTOR, PROCESS FOR PREPARING THE SAME, AND METHOD FOR REALIZING BIOCHEMICAL OR BIOLOGICAL REACTION
WO2002101543A3 (en) Tool and programming method
WO2002092221A8 (en) A system for performing experiments, in particular for high throughput experimentation
WO2003012116A3 (en) Caax prenyl protease nucleic acids and polypeptides and methods of use thereof
Lyttle Combinatorial chemistry: a conservative perspective

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: C2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/17-17/17, DRAWINGS, REPLACED BY NEW PAGES 1/17-17/17; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

Free format text: PAGES 1/17-17/17, DRAWINGS, REPLACED BY NEW PAGES 1/17-17/17; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

WWE Wipo information: entry into national phase

Ref document number: 10203235

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP