WO2002052276A3 - Protein s functional assay - Google Patents

Protein s functional assay Download PDF

Info

Publication number
WO2002052276A3
WO2002052276A3 PCT/US2001/050338 US0150338W WO02052276A3 WO 2002052276 A3 WO2002052276 A3 WO 2002052276A3 US 0150338 W US0150338 W US 0150338W WO 02052276 A3 WO02052276 A3 WO 02052276A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
clotting time
assay
purified
functional assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/050338
Other languages
French (fr)
Other versions
WO2002052276A2 (en
WO2002052276A9 (en
Inventor
Yong Dai
Biqing Ye
Kui Chen
Pau Bruguera
Shamay Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Werfen North America
Original Assignee
Instrumentation Laboratory Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instrumentation Laboratory Co filed Critical Instrumentation Laboratory Co
Priority to JP2002553124A priority Critical patent/JP2004528534A/en
Priority to EP01994446A priority patent/EP1344066B1/en
Priority to CA002432118A priority patent/CA2432118C/en
Priority to AU2002246838A priority patent/AU2002246838B2/en
Priority to DE60139572T priority patent/DE60139572D1/en
Priority to AT01994446T priority patent/ATE439601T1/en
Publication of WO2002052276A2 publication Critical patent/WO2002052276A2/en
Publication of WO2002052276A3 publication Critical patent/WO2002052276A3/en
Anticipated expiration legal-status Critical
Publication of WO2002052276A9 publication Critical patent/WO2002052276A9/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates generally to a new functional protein S assay and kit that is based on the ability of endogenous protein S to prolong clotting time. In the assay procedure, a test plasma sample is diluted with protein S deficient plasma, followed by the addition of purified or recombinant tissue factor (pTF or rTF), purified natural or synthetic phospholipid (pPL or sPL) and activated protein C (APC) or protein C activator (PCA). The clotting time is then measured and compared to a standard curve or a normal control.
PCT/US2001/050338 2000-12-19 2001-12-19 Protein s functional assay Ceased WO2002052276A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2002553124A JP2004528534A (en) 2000-12-19 2001-12-19 Protein S functional assay
EP01994446A EP1344066B1 (en) 2000-12-19 2001-12-19 Protein s functional assay
CA002432118A CA2432118C (en) 2000-12-19 2001-12-19 Protein s functional assay
AU2002246838A AU2002246838B2 (en) 2000-12-19 2001-12-19 Protein S functional assay
DE60139572T DE60139572D1 (en) 2000-12-19 2001-12-19 FUNCTIONAL ASSAY FOR PROTEIN S
AT01994446T ATE439601T1 (en) 2000-12-19 2001-12-19 FUNCTIONAL ASSAY FOR PROTEIN S

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25670300P 2000-12-19 2000-12-19
US60/256,703 2000-12-19

Publications (3)

Publication Number Publication Date
WO2002052276A2 WO2002052276A2 (en) 2002-07-04
WO2002052276A3 true WO2002052276A3 (en) 2003-04-17
WO2002052276A9 WO2002052276A9 (en) 2003-07-31

Family

ID=22973254

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050338 Ceased WO2002052276A2 (en) 2000-12-19 2001-12-19 Protein s functional assay

Country Status (8)

Country Link
EP (1) EP1344066B1 (en)
JP (1) JP2004528534A (en)
AT (1) ATE439601T1 (en)
AU (1) AU2002246838B2 (en)
CA (1) CA2432118C (en)
DE (1) DE60139572D1 (en)
ES (1) ES2328453T3 (en)
WO (1) WO2002052276A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2922024B1 (en) * 2007-10-04 2011-03-25 Stago Diagnostica METHOD FOR ADJUSTING THE CALIBRATION OF DIAGNOSTIC TESTS
CN102597777A (en) * 2009-05-07 2012-07-18 国家健康与医学研究院 Methods for diagnosing thrombophilia
JPWO2012124798A1 (en) * 2011-03-14 2014-07-24 株式会社シノテスト Reagent for measuring activity of total protein S in sample and method for measuring activity
EP3414576B1 (en) * 2016-02-12 2024-04-03 Instrumentation Laboratory Company A two component "mix and use" liquid thromboplastin reagent, methods of making, and methods of use thereof
CN106546574A (en) * 2016-10-28 2017-03-29 山西太钢不锈钢股份有限公司 The spectrum analyses and bearing calibration of trace nitrogen in a kind of steel
IL257229B (en) * 2018-01-30 2022-07-01 Reddress Ltd A blood-conducting device for tissue treatment
SG11202100132UA (en) * 2018-07-06 2021-02-25 Lsi Medience Corp Liposome-containing liquid reagent for measuring blood coagulation ability
WO2025132651A1 (en) * 2023-12-20 2025-06-26 Pentapharm Ag Method for analyzing a blood sample with respect to a hemostatic regulator

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0406971A1 (en) * 1989-07-07 1991-01-09 INSTRUMENTATION LABORATORY S.p.A. Method and kit for determining the functional activity of protein S in human plasma
EP0445626A2 (en) * 1990-03-03 1991-09-11 BEHRINGWERKE Aktiengesellschaft Functional test for determining protein S activity
EP0696642A1 (en) * 1994-08-08 1996-02-14 BEHRINGWERKE Aktiengesellschaft Process for the detection of disorders of the protein C/protein S system
US5780255A (en) * 1995-06-09 1998-07-14 Instrumentation Laboratory, S.P.A. Protein C pathway screening test

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0406971A1 (en) * 1989-07-07 1991-01-09 INSTRUMENTATION LABORATORY S.p.A. Method and kit for determining the functional activity of protein S in human plasma
EP0445626A2 (en) * 1990-03-03 1991-09-11 BEHRINGWERKE Aktiengesellschaft Functional test for determining protein S activity
EP0696642A1 (en) * 1994-08-08 1996-02-14 BEHRINGWERKE Aktiengesellschaft Process for the detection of disorders of the protein C/protein S system
US5780255A (en) * 1995-06-09 1998-07-14 Instrumentation Laboratory, S.P.A. Protein C pathway screening test

Also Published As

Publication number Publication date
WO2002052276A2 (en) 2002-07-04
ATE439601T1 (en) 2009-08-15
CA2432118C (en) 2008-12-09
WO2002052276A9 (en) 2003-07-31
JP2004528534A (en) 2004-09-16
CA2432118A1 (en) 2002-07-04
EP1344066B1 (en) 2009-08-12
DE60139572D1 (en) 2009-09-24
AU2002246838B2 (en) 2006-12-07
ES2328453T3 (en) 2009-11-13
EP1344066A2 (en) 2003-09-17

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