WO2002052034A8 - Bioscopy method and method of nucleic acid amplification - Google Patents

Bioscopy method and method of nucleic acid amplification

Info

Publication number
WO2002052034A8
WO2002052034A8 PCT/JP2001/011489 JP0111489W WO02052034A8 WO 2002052034 A8 WO2002052034 A8 WO 2002052034A8 JP 0111489 W JP0111489 W JP 0111489W WO 02052034 A8 WO02052034 A8 WO 02052034A8
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
cells
bioscopy
dna
acid amplification
Prior art date
Application number
PCT/JP2001/011489
Other languages
French (fr)
Japanese (ja)
Other versions
WO2002052034A1 (en
Inventor
Joji Oshima
Original Assignee
Joji Oshima
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joji Oshima filed Critical Joji Oshima
Priority to JP2002553513A priority Critical patent/JPWO2002052034A1/en
Publication of WO2002052034A1 publication Critical patent/WO2002052034A1/en
Publication of WO2002052034A8 publication Critical patent/WO2002052034A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

A bioscopy method whereby life or death of cells and/or bacteria is judged by applying nucleic acid quantification; and a method of nucleic acid amplification whereby cells and/or nucleic acid genes are efficiently and directly amplified. For example, DNA stable and unstable parts in samples containing viable cells and dead cells at different mixing ratios are amplified by PCR. Then PCR products are observed in a sample A wherein the DNA unstable part comprises viable cells exclusively while no PCR product is observed in a sample H wherein the DNA unstable part comprises dead cells exclusively. Thus, the life/death and mixing ratios of the samples can be understood.
PCT/JP2001/011489 2000-12-26 2001-12-26 Bioscopy method and method of nucleic acid amplification WO2002052034A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2002553513A JPWO2002052034A1 (en) 2000-12-26 2001-12-26 Life and death discrimination method and nucleic acid amplification method

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP2000-404592 2000-12-26
JP2000404592 2000-12-26
JP2001060901 2001-01-30
JP2001-60901 2001-01-30
JP2001-205386 2001-06-04
JP2001205386 2001-06-04

Publications (2)

Publication Number Publication Date
WO2002052034A1 WO2002052034A1 (en) 2002-07-04
WO2002052034A8 true WO2002052034A8 (en) 2002-08-29

Family

ID=27345654

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/011489 WO2002052034A1 (en) 2000-12-26 2001-12-26 Bioscopy method and method of nucleic acid amplification

Country Status (2)

Country Link
JP (1) JPWO2002052034A1 (en)
WO (1) WO2002052034A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1992690B1 (en) 2006-02-17 2014-05-21 Morinaga Milk Industry Co., Ltd. Method for detection of microorganism and kit for detection of microorganism
KR101120270B1 (en) 2007-08-16 2012-03-06 모리나가 뉴교 가부시키가이샤 Method and kit for detection of microorganism
JP2010046016A (en) * 2008-08-21 2010-03-04 Toyo Seikan Kaisha Ltd Method for microorganism detection
MX340406B (en) 2009-01-15 2016-07-07 Nat Univ Corp Univ Of Toyama Enzymatic preparation containing thermostable dna polymerase, process for producing same, and method for detecting analyte organism.
AU2010275576B2 (en) 2009-07-24 2013-02-28 Morinaga Milk Industry Co., Ltd. Method and kit for detection of microorganism
WO2012092238A1 (en) * 2010-12-31 2012-07-05 Zeus Scientific, Inc. Improved methods for determining cell viability using molecular nucleic acid-based techniques
DE102012014981B3 (en) 2012-07-26 2013-07-18 Sartorius Stedim Biotech Gmbh Method for differentiation between living and dead cells
CN109207616A (en) * 2018-11-14 2019-01-15 北京工业大学 A kind of method of dead bacterium or suspend mode bacterium in detection activated sludge

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10234377A (en) * 1997-02-27 1998-09-08 Asahi Breweries Ltd Oligonucleotide for detecting lactobacillus and detection of the bacterium
FR2766825B1 (en) * 1997-08-04 2001-04-13 Pasteur Institut SPECIFIC OLIGONUCLEOTIDE OF THE SPECIES ESCHERICHIA COLI AND METHOD FOR THE DETECTION AND VISUALIZATION OF THE BACTERIA OF THIS SPECIES
ES2275336T3 (en) * 1998-01-23 2007-06-01 Biomerieux B.V. EF-TU mRNA TO DETERMINE THE VIABILITY OF BACTERIA.

Also Published As

Publication number Publication date
JPWO2002052034A1 (en) 2004-04-30
WO2002052034A1 (en) 2002-07-04

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