WO2002051982A3 - Bacterial promoters and methods of use - Google Patents

Bacterial promoters and methods of use Download PDF

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Publication number
WO2002051982A3
WO2002051982A3 PCT/US2001/050250 US0150250W WO02051982A3 WO 2002051982 A3 WO2002051982 A3 WO 2002051982A3 US 0150250 W US0150250 W US 0150250W WO 02051982 A3 WO02051982 A3 WO 02051982A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
fusion promoters
promoters
fusion
embodiments include
Prior art date
Application number
PCT/US2001/050250
Other languages
French (fr)
Other versions
WO2002051982A2 (en
Inventor
Robert Haselbeck
Dan Wall
Molly Gross
Original Assignee
Elitra Pharmaceuticals Inc
Robert Haselbeck
Dan Wall
Molly Gross
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elitra Pharmaceuticals Inc, Robert Haselbeck, Dan Wall, Molly Gross filed Critical Elitra Pharmaceuticals Inc
Publication of WO2002051982A2 publication Critical patent/WO2002051982A2/en
Publication of WO2002051982A3 publication Critical patent/WO2002051982A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Abstract

Compositions and methods are disclosed herein that relate to the development of fusion promoters for regulating gene expression in bacteria. Embodiments include fusion promoters comprising one or more operators linked to a promoter that is modified to have altered activity in Gram-positive organisms. Vectors and cells containing these fusion promoters are also described. Other embodiments include, methods of using these fusion promoters to regulate nucleic acid and/or polypeptide expression, methods of using these fusion promoters to identify proliferation-required genes, and methods of using these fusion promoters to identify molecules having potential antibiotic activity.
PCT/US2001/050250 2000-12-27 2001-12-21 Bacterial promoters and methods of use WO2002051982A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US25943400P 2000-12-27 2000-12-27
US60/259,434 2000-12-27
US94899301A 2001-09-06 2001-09-06
US09/948,993 2001-09-06

Publications (2)

Publication Number Publication Date
WO2002051982A2 WO2002051982A2 (en) 2002-07-04
WO2002051982A3 true WO2002051982A3 (en) 2009-08-06

Family

ID=22864826

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050250 WO2002051982A2 (en) 2000-12-27 2001-12-21 Bacterial promoters and methods of use

Country Status (2)

Country Link
AU (1) AU2002241706A1 (en)
WO (1) WO2002051982A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110195075B (en) * 2019-05-21 2023-02-28 天津科技大学 Streptococcus gene induction expression system and application thereof
WO2023166132A1 (en) * 2022-03-04 2023-09-07 Gen-H Genetic Engineering Heidelberg Gmbh Microorganism strain for antibiotic-free plasmid-based fermentation and method for generation thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0186069A2 (en) * 1984-12-17 1986-07-02 F. Hoffmann-La Roche Ag New expression control sequence
US4689406A (en) * 1983-08-10 1987-08-25 Amgen Enhancement of microbial expression of polypeptides
WO1999028508A1 (en) * 1997-12-04 1999-06-10 Smithkline Beecham Corporation Method of generating conditionally expressed mutant cells using expressible antisense sequences
US6017730A (en) * 1992-04-06 2000-01-25 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their environment
US6303338B1 (en) * 1996-08-16 2001-10-16 Human Genome Sciences, Inc. Pancreas-derived plasminogen activator inhibitor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4689406A (en) * 1983-08-10 1987-08-25 Amgen Enhancement of microbial expression of polypeptides
EP0186069A2 (en) * 1984-12-17 1986-07-02 F. Hoffmann-La Roche Ag New expression control sequence
US6017730A (en) * 1992-04-06 2000-01-25 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their environment
US6303338B1 (en) * 1996-08-16 2001-10-16 Human Genome Sciences, Inc. Pancreas-derived plasminogen activator inhibitor
WO1999028508A1 (en) * 1997-12-04 1999-06-10 Smithkline Beecham Corporation Method of generating conditionally expressed mutant cells using expressible antisense sequences

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GARTNER ET AL.: "Regualtion of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose", MOL. GEN. GENET., vol. 232, May 1992 (1992-05-01), pages 415 - 422 *
WEILAND ET AL.: "A promoter-screening plasmid and xylose-inducible, glucose- repressible expression vectors for Staphylococcus carnosus", GENE, vol. 158, June 1995 (1995-06-01), pages 91 - 96 *

Also Published As

Publication number Publication date
WO2002051982A2 (en) 2002-07-04
AU2002241706A1 (en) 2002-07-08

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