WO2002048187A3 - Peptides du signal de secretion, leurs sequences adn, vecteurs d'expression pour cellules eucaryotes preparees a partir de ceux-ci, et leur utilisation pour la preparation biotechnologique de proteines - Google Patents
Peptides du signal de secretion, leurs sequences adn, vecteurs d'expression pour cellules eucaryotes preparees a partir de ceux-ci, et leur utilisation pour la preparation biotechnologique de proteinesInfo
- Publication number
- WO2002048187A3 WO2002048187A3 PCT/EP2001/014588 EP0114588W WO0248187A3 WO 2002048187 A3 WO2002048187 A3 WO 2002048187A3 EP 0114588 W EP0114588 W EP 0114588W WO 0248187 A3 WO0248187 A3 WO 0248187A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- eukaryotic cells
- proteins
- same
- expression vectors
- secretion signal
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2002235771A AU2002235771A1 (en) | 2000-12-14 | 2001-12-12 | Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE2000162302 DE10062302A1 (de) | 2000-12-14 | 2000-12-14 | Sekretionssignalpeptide, deren DNA-Sequenzen, damit herstellbare Expressionsvektoren für eukaryotische Zellen und deren Verwendung zur biotechnologischen Herstellung von Proteinen |
DE10062302.6 | 2000-12-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002048187A2 WO2002048187A2 (fr) | 2002-06-20 |
WO2002048187A3 true WO2002048187A3 (fr) | 2003-01-30 |
Family
ID=7667112
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2001/014588 WO2002048187A2 (fr) | 2000-12-14 | 2001-12-12 | Peptides du signal de secretion, leurs sequences adn, vecteurs d'expression pour cellules eucaryotes preparees a partir de ceux-ci, et leur utilisation pour la preparation biotechnologique de proteines |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2002235771A1 (fr) |
DE (1) | DE10062302A1 (fr) |
WO (1) | WO2002048187A2 (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10252245A1 (de) * | 2002-11-07 | 2004-05-27 | Prof. Dr. Danilo Porro Università degli Studi di Milano-Bicocca Dipartimento die Biotechnologie e Bioscienze | Verfahren zur Expression und Sekretion von Proteinen mittels der nicht-konventionellen Hefe Zygosaccharomyces bailii |
US8815580B2 (en) | 2008-08-08 | 2014-08-26 | Vib Vzw | Cells producing glycoproteins having altered glycosylation patterns and method and use thereof |
EP2617732A1 (fr) | 2012-01-19 | 2013-07-24 | Vib Vzw | Outils et procédés pour l'expression de protéines de membrane |
WO2015032899A1 (fr) | 2013-09-05 | 2015-03-12 | Vib Vzw | Cellules produisant des molécules contenant fc et présentant des motifs de glycosylation modifiés et leurs procédés d'utilisation |
US11293012B2 (en) | 2015-07-09 | 2022-04-05 | Vib Vzw | Cells producing glycoproteins having altered N- and O-glycosylation patterns and methods and use thereof |
GB201901608D0 (en) | 2019-02-06 | 2019-03-27 | Vib Vzw | Vaccine adjuvant conjugates |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998054339A1 (fr) * | 1997-05-27 | 1998-12-03 | Hanil Synthetic Fiber Co., Ltd. | TECHNIQUE DE MISE AU POINT DE PROTEINES DE RECOMBINAISON FAISANT INTERVENIR UN VECTEUR D'EXPRESSION DES PLUS EFFICACE ISSU DE $i(SACCHAROMYCES CEREVISIAE) |
-
2000
- 2000-12-14 DE DE2000162302 patent/DE10062302A1/de not_active Ceased
-
2001
- 2001-12-12 AU AU2002235771A patent/AU2002235771A1/en not_active Abandoned
- 2001-12-12 WO PCT/EP2001/014588 patent/WO2002048187A2/fr not_active Application Discontinuation
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998054339A1 (fr) * | 1997-05-27 | 1998-12-03 | Hanil Synthetic Fiber Co., Ltd. | TECHNIQUE DE MISE AU POINT DE PROTEINES DE RECOMBINAISON FAISANT INTERVENIR UN VECTEUR D'EXPRESSION DES PLUS EFFICACE ISSU DE $i(SACCHAROMYCES CEREVISIAE) |
Non-Patent Citations (10)
Title |
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BASI G ET AL: "TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility.", GENE. NETHERLANDS 15 JAN 1993, vol. 123, no. 1, 15 January 1993 (1993-01-15), pages 131 - 136, XP002211006, ISSN: 0378-1119 * |
CARTWRIGHT C P ET AL: "Use of beta-lactamase as a secreted reporter of promoter function in yeast.", YEAST (CHICHESTER, ENGLAND) ENGLAND APR 1994, vol. 10, no. 4, April 1994 (1994-04-01), pages 497 - 508, XP001089672, ISSN: 0749-503X * |
HEINTEL T ET AL: "Expression, processing and high level secretion of a virus toxin in fission yeast.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. GERMANY JUL 2001, vol. 56, no. 1-2, July 2001 (2001-07-01), pages 165 - 172, XP002211010, ISSN: 0175-7598 * |
LEE JEEWON ET AL: "Novel secretion system of recombinant Saccharomyces cerevisiae using an N-terminus residue of human IL-1beta as secretion enhancer.", BIOTECHNOLOGY PROGRESS, vol. 15, no. 5, 1999, pages 884 - 890, XP002211009, ISSN: 8756-7938 * |
MAUNDRELL K: "nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 5 JUL 1990, vol. 265, no. 19, 5 July 1990 (1990-07-05), pages 10857 - 10864, XP002211007, ISSN: 0021-9258 * |
MAUNDRELL K: "Thiamine-repressible expression vectors pREP and pRIP for fission yeast.", GENE. NETHERLANDS 15 JAN 1993, vol. 123, no. 1, 15 January 1993 (1993-01-15), pages 127 - 130, XP001074164, ISSN: 0378-1119 * |
SCHMITT M J ET AL: "Sequence of the M28 dsRNA: preprotoxin is processed to an alpha/beta heterodimeric protein toxin.", VIROLOGY. UNITED STATES 10 NOV 1995, vol. 213, no. 2, 10 November 1995 (1995-11-10), pages 341 - 351, XP002211003, ISSN: 0042-6822 * |
SCHMITT MANFRED J: "Cloning and expression of a cDNA copy of the viral K-28 killer toxin gene in yeast.", MOLECULAR & GENERAL GENETICS, vol. 246, no. 2, 1995, pages 236 - 246, XP001074299, ISSN: 0026-8925 * |
SCMITT, J. M. & EISFELD, K.: "'Killer' viruses in Saccharamyces cerevisiae and their general importance in understanding eukaryotic cell biology.", RECENT RES. VIROL., vol. 1, 1999, pages 525 - 545, XP008007237 * |
TOKUNAGA M ET AL: "STRUCTURE OF YEAST PGKL 128-KDA KILLER-TOXIN SECRETION SIGNAL SEQUENCE PROCESSING OF THE 128-KDA KILLER-TOXIN-SECRETION-SIGNAL-ALPHA-AMYLASE FUSION PROTEIN", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 203, no. 3, 1992, pages 415 - 424, XP008007305, ISSN: 0014-2956 * |
Also Published As
Publication number | Publication date |
---|---|
DE10062302A1 (de) | 2002-07-11 |
AU2002235771A1 (en) | 2002-06-24 |
WO2002048187A2 (fr) | 2002-06-20 |
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