WO2002046754A3 - Functional proteomics using double phage display screening - Google Patents

Functional proteomics using double phage display screening Download PDF

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Publication number
WO2002046754A3
WO2002046754A3 PCT/US2001/047836 US0147836W WO0246754A3 WO 2002046754 A3 WO2002046754 A3 WO 2002046754A3 US 0147836 W US0147836 W US 0147836W WO 0246754 A3 WO0246754 A3 WO 0246754A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
antibody
phagemid
library
specific
Prior art date
Application number
PCT/US2001/047836
Other languages
French (fr)
Other versions
WO2002046754A2 (en
Inventor
Robert S Gilmour
Keith Wright Marvin
Original Assignee
Neuronz Ltd
Robert S Gilmour
Keith Wright Marvin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neuronz Ltd, Robert S Gilmour, Keith Wright Marvin filed Critical Neuronz Ltd
Priority to US10/433,738 priority Critical patent/US20040053218A1/en
Priority to AU2002230749A priority patent/AU2002230749A1/en
Publication of WO2002046754A2 publication Critical patent/WO2002046754A2/en
Publication of WO2002046754A3 publication Critical patent/WO2002046754A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to protein identification. In one aspect, the invention provides a method for the identification of a first protein or of a second protein containing an idiotypic region of the first protein comprising (a) contacting said first protein with an antibody phagemid library to form a complex between said first protein and at least one member of said library, (b) screening a cDNA phagemid library of the proteome of a tissue of interest with the complex forming antibody to identify a protein-specific phagemid that displays a protein that binds the antibody phagemidi, (c) identifying the second protein from the cDNA of the protein-specific phagemid. In a second aspect, the method involves as step (b) screening a ribosome display library of the proteome of the tissue of interest with the complex forming antibody obtained from step (a) to identify a protein-specific ribosome that dis plays a protein that binds the antibody phagemid. If the antibody phage yield is low, the phage DNA may be amplified prior to reinfecting bacteria.
PCT/US2001/047836 2000-12-08 2001-12-07 Functional proteomics using double phage display screening WO2002046754A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/433,738 US20040053218A1 (en) 2001-12-07 2001-12-07 Functional proteomics using double phage display screening
AU2002230749A AU2002230749A1 (en) 2000-12-08 2001-12-07 Functional proteomics using double phage display screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25446600P 2000-12-08 2000-12-08
US60/254,466 2000-12-08

Publications (2)

Publication Number Publication Date
WO2002046754A2 WO2002046754A2 (en) 2002-06-13
WO2002046754A3 true WO2002046754A3 (en) 2003-05-22

Family

ID=22964415

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/047836 WO2002046754A2 (en) 2000-12-08 2001-12-07 Functional proteomics using double phage display screening

Country Status (2)

Country Link
AU (1) AU2002230749A1 (en)
WO (1) WO2002046754A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999039210A1 (en) * 1998-01-29 1999-08-05 Miller, Samuel High density arrays for proteome analysis and methods and compositions therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999039210A1 (en) * 1998-01-29 1999-08-05 Miller, Samuel High density arrays for proteome analysis and methods and compositions therefor

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
LITTLE M ET AL: "Generation of a large complex antibody library from multiple donors", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 231, no. 1-2, 10 December 1999 (1999-12-10), pages 3 - 9, XP004187630, ISSN: 0022-1759 *
LIU BIN ET AL: "Applying phage antibodies to proteomics: Selecting single chain Fv antibodies to antigens blotted on nitrocellulose", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 286, no. 1, 1 November 2000 (2000-11-01), pages 119 - 128, XP002187878, ISSN: 0003-2697 *
LUFT B J ET AL: "The 93-kilodalton protein of Borrelia burgdorferi: an immunodominant protoplasmic cylinder antigen", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, US, vol. 60, no. 10, October 1992 (1992-10-01), pages 4309 - 4321, XP002127125, ISSN: 0019-9567 *
SCHAFFITZEL C ET AL: "Ribosome display: an in vitro method for selection and evolution of antibodies from libraries", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 231, no. 1-2, 10 December 1999 (1999-12-10), pages 119 - 135, XP004187639, ISSN: 0022-1759 *
SOERENSEN A ET AL: "PURIFICATION AND CHARACTERIZATION OF A LOW-MOLECULAR-MASS T-CELL ANTIGEN SECRETED BY MYCOBACTERIUM TUBERCULOSIS", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, US, vol. 63, no. 5, 1 May 1995 (1995-05-01), pages 1710 - 1717, XP002068818, ISSN: 0019-9567 *

Also Published As

Publication number Publication date
AU2002230749A1 (en) 2002-06-18
WO2002046754A2 (en) 2002-06-13

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