WO2002044204A3 - Cell-free assembly of lentiviral capsids - Google Patents

Cell-free assembly of lentiviral capsids Download PDF

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Publication number
WO2002044204A3
WO2002044204A3 PCT/US2001/046919 US0146919W WO0244204A3 WO 2002044204 A3 WO2002044204 A3 WO 2002044204A3 US 0146919 W US0146919 W US 0146919W WO 0244204 A3 WO0244204 A3 WO 0244204A3
Authority
WO
WIPO (PCT)
Prior art keywords
capsids
lentiviral
methods
assembly
compounds
Prior art date
Application number
PCT/US2001/046919
Other languages
French (fr)
Other versions
WO2002044204A2 (en
Inventor
Stephen Campbell
Alan Rein
Eric Towler
Robert Fisher
Haleem J Issaq
Original Assignee
Us Gov Health & Human Serv
Stephen Campbell
Alan Rein
Eric Towler
Robert Fisher
Haleem J Issaq
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Stephen Campbell, Alan Rein, Eric Towler, Robert Fisher, Haleem J Issaq filed Critical Us Gov Health & Human Serv
Priority to AU2002230647A priority Critical patent/AU2002230647A1/en
Publication of WO2002044204A2 publication Critical patent/WO2002044204A2/en
Publication of WO2002044204A3 publication Critical patent/WO2002044204A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15023—Virus like particles [VLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods and compositions for assembling full-sized, stable lentiviral capsids from purified lentiviral Gag polypeptides in vitro are disclosed. The inclusion of phosphorylated compounds in the assembly reaction yields spherical particles indistinguishable from the viral capsids produced by infected cells. Such particles are useful to deliver nucleic acids or other therapeutic molecules to cells. Also provided are methods to identify other compounds that promote capsid assembly, as well as methods to identify therapeutic compounds that interfere with viral assembly. Pharmaceutical compositions containing the in vitro assembled lentiviral capsids are also provided.
PCT/US2001/046919 2000-12-01 2001-11-30 Cell-free assembly of lentiviral capsids WO2002044204A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002230647A AU2002230647A1 (en) 2000-12-01 2001-11-30 Cell-free assembly of lentiviral capsids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25078600P 2000-12-01 2000-12-01
US60/250,786 2000-12-01

Publications (2)

Publication Number Publication Date
WO2002044204A2 WO2002044204A2 (en) 2002-06-06
WO2002044204A3 true WO2002044204A3 (en) 2003-09-12

Family

ID=22949141

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046919 WO2002044204A2 (en) 2000-12-01 2001-11-30 Cell-free assembly of lentiviral capsids

Country Status (2)

Country Link
AU (1) AU2002230647A1 (en)
WO (1) WO2002044204A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008500364A (en) * 2004-05-25 2008-01-10 キメラコア， インコーポレイテッド Self-assembling nanoparticle drug delivery system
WO2008108776A1 (en) 2006-04-07 2008-09-12 Chimeros, Inc. Compositions and methods for treating b- cell malignancies
WO2010120874A2 (en) 2009-04-14 2010-10-21 Chimeros, Inc. Chimeric therapeutics, compositions, and methods for using same
JP2014527072A (en) 2011-09-09 2014-10-09 バイオメド リアルティー， エル．ピー． Methods and compositions for controlling the assembly of viral proteins

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035062A1 (en) * 1997-02-07 1998-08-13 Lingappa Jaisri R Multistep, atp-dependent cell-free system for the assembly of human immunodeficiency virus capsids
US6099847A (en) * 1997-05-15 2000-08-08 The United States Of America As Represented By The Department Of Health And Human Services Chimeric Gag pseudovirions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035062A1 (en) * 1997-02-07 1998-08-13 Lingappa Jaisri R Multistep, atp-dependent cell-free system for the assembly of human immunodeficiency virus capsids
US6099847A (en) * 1997-05-15 2000-08-08 The United States Of America As Represented By The Department Of Health And Human Services Chimeric Gag pseudovirions

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CAMPBELL STEPHEN ET AL: "In vitro assembly properties of human immunodeficiency virus type 1 Gag protein lacking the p6 domain.", JOURNAL OF VIROLOGY, vol. 73, no. 3, March 1999 (1999-03-01), pages 2270 - 2279, XP002236517, ISSN: 0022-538X *
CAMPBELL STEPHEN ET AL: "Modulation of HIV-like particle assembly in vitro by inositol phosphates.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 19, 11 September 2001 (2001-09-11), September 11, 2001, pages 10875 - 10879, XP002236518, ISSN: 0027-8424 *
GROSS INGOLF ET AL: "In vitro assembly properties of purified bacterially expressed capsid proteins of human immunodeficiency virus.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 249, no. 2, 1997, pages 592 - 600, XP008015567, ISSN: 0014-2956 *
NGUYEN DZUNG H ET AL: "Evidence for budding of human immunodeficiency virus type 1 selectively from glycolipid-enriched membrane lipid rafts.", JOURNAL OF VIROLOGY, vol. 74, no. 7, April 2000 (2000-04-01), pages 3264 - 3272, XP002236519, ISSN: 0022-538X *

Also Published As

Publication number Publication date
AU2002230647A1 (en) 2002-06-11
WO2002044204A2 (en) 2002-06-06

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