WO2002042311A3 - Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs - Google Patents

Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs Download PDF

Info

Publication number
WO2002042311A3
WO2002042311A3 PCT/FR2001/003669 FR0103669W WO0242311A3 WO 2002042311 A3 WO2002042311 A3 WO 2002042311A3 FR 0103669 W FR0103669 W FR 0103669W WO 0242311 A3 WO0242311 A3 WO 0242311A3
Authority
WO
WIPO (PCT)
Prior art keywords
vectors
carbon atoms
biotin derivatives
chain
methods
Prior art date
Application number
PCT/FR2001/003669
Other languages
English (en)
Other versions
WO2002042311A2 (fr
Inventor
Abdelakim Kharrat
Khalil Bouayadi
Jean-Charles Faye
Emmanuelle Fourcade
Rudi Baron
Daniel Tovar
Casimir Blonski
Original Assignee
Inst Nat Sante Rech Med
Millegen Sarl
Abdelakim Kharrat
Khalil Bouayadi
Jean-Charles Faye
Emmanuelle Fourcade
Rudi Baron
Daniel Tovar
Casimir Blonski
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Nat Sante Rech Med, Millegen Sarl, Abdelakim Kharrat, Khalil Bouayadi, Jean-Charles Faye, Emmanuelle Fourcade, Rudi Baron, Daniel Tovar, Casimir Blonski, Centre Nat Rech Scient filed Critical Inst Nat Sante Rech Med
Priority to AU2002222004A priority Critical patent/AU2002222004A1/en
Priority to EP01997495A priority patent/EP1347985A2/fr
Priority to US10/432,383 priority patent/US20040138446A1/en
Publication of WO2002042311A2 publication Critical patent/WO2002042311A2/fr
Publication of WO2002042311A3 publication Critical patent/WO2002042311A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/415—1,2-Diazoles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/555—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound pre-targeting systems involving an organic compound, other than a peptide, protein or antibody, for targeting specific cells
    • A61K47/557—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound pre-targeting systems involving an organic compound, other than a peptide, protein or antibody, for targeting specific cells the modifying agent being biotin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/185—Heterocyclic compounds containing sulfur atoms as ring hetero atoms in the condensed system
    • C12P17/186—Heterocyclic compounds containing sulfur atoms as ring hetero atoms in the condensed system containing a 2-oxo-thieno[3,4-d]imidazol nucleus, e.g. Biotin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Dérivés de la biotine de formule générale (I) suivante, dans laquelle: R1 représente un composé déterminé, X1 représente une chaîne carbonnée, de préférence de 1 à 10 atomes de carbone, substituée ou non, comportant le cas échéant un ou plusieurs groupes carbonyle ou oxycarbonyle, et/ou un groupe -NH- notamment une chaîne de formule -HN(CH2)n-O-CO- dans laquelle n est un nombre entier de 1 à 5 et comportant le cas échéant une ou des fonctions ether, amide, et/ou amine. Y représente une chaîne hycdrocarbonée de 1 à 5 atomes de carbone, substituée ou non, R2 représente un atome d'hydrogène, ou un groupement protecteur, notamment R2 représente un groupe alkyle de l à 5 atomes de carbone. Application: utilisation en tant que vecteurs, notamment dans le cadre de la mise en oeuvre de méthodes de détection d'interactions entre des composés biologiques, ainsi que dans des compositions pharmaceutiques.
PCT/FR2001/003669 2000-11-22 2001-11-21 Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs WO2002042311A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002222004A AU2002222004A1 (en) 2000-11-22 2001-11-21 Biotin derivatives, methods for making same and uses thereof as vectors
EP01997495A EP1347985A2 (fr) 2000-11-22 2001-11-21 Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs
US10/432,383 US20040138446A1 (en) 2000-11-22 2001-11-21 Biotin derivatives, methods for making same and uses thereof as vectors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0015049 2000-11-22
FR0015049A FR2816943B1 (fr) 2000-11-22 2000-11-22 Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs

Publications (2)

Publication Number Publication Date
WO2002042311A2 WO2002042311A2 (fr) 2002-05-30
WO2002042311A3 true WO2002042311A3 (fr) 2002-09-19

Family

ID=8856739

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2001/003669 WO2002042311A2 (fr) 2000-11-22 2001-11-21 Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs

Country Status (5)

Country Link
US (1) US20040138446A1 (fr)
EP (1) EP1347985A2 (fr)
AU (1) AU2002222004A1 (fr)
FR (1) FR2816943B1 (fr)
WO (1) WO2002042311A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002097197A (ja) * 2000-07-18 2002-04-02 Yanaihara Kenkyusho:Kk エストラジオール誘導体およびこれを使用した測定法
US8338648B2 (en) * 2004-06-12 2012-12-25 Signum Biosciences, Inc. Topical compositions and methods for epithelial-related conditions
EP2324076B1 (fr) 2008-07-31 2017-11-01 3M Innovative Properties Company Compositions à base de fluoropolymère et leur procédé de fabrication et d utilisation
WO2010014276A1 (fr) * 2008-07-31 2010-02-04 3M Innovative Properties Company Compositions d'azoture et leurs procédés de préparation et d'utilisation
KR20210126136A (ko) 2016-12-27 2021-10-19 에프. 호프만-라 로슈 아게 신규한 비오틴-특이적 단일클론 항체 및 그 용도

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0254646A1 (fr) * 1986-07-22 1988-01-27 Institut Pasteur Sondes de détection d'acides nucléiques renfermant des dérivés de désoxy-2' adénosine
WO1991007087A1 (fr) * 1989-11-13 1991-05-30 Affymax Technologies N.V. Immobilisation adressable dans l'espace d'anti-ligands sur des surfaces
US5128476A (en) * 1991-02-20 1992-07-07 The Midland Certified Reagent Company Biotinylated oligonucleotides and reagents for preparing the same
WO1995004069A1 (fr) * 1993-07-30 1995-02-09 Affymax Technologies N.V. Biotinylation de proteines
WO1995025172A1 (fr) * 1994-03-17 1995-09-21 Universite Louis Pasteur Fragments d'anticorps recombinants synthetises et biotinyles dans e. coli, leur utilisation en immunodosages et en purification par immunoaffinite
WO2000040712A1 (fr) * 1999-01-07 2000-07-13 Medical Research Council Technique de tri optique

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252743A (en) * 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US6242610B1 (en) * 1999-05-27 2001-06-05 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Derivatized biotin compounds and methods of use
EP1111050B1 (fr) * 1999-12-22 2007-03-14 Dade Behring Marburg GmbH Solutions de procalcitonine humaine

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0254646A1 (fr) * 1986-07-22 1988-01-27 Institut Pasteur Sondes de détection d'acides nucléiques renfermant des dérivés de désoxy-2' adénosine
WO1991007087A1 (fr) * 1989-11-13 1991-05-30 Affymax Technologies N.V. Immobilisation adressable dans l'espace d'anti-ligands sur des surfaces
US5128476A (en) * 1991-02-20 1992-07-07 The Midland Certified Reagent Company Biotinylated oligonucleotides and reagents for preparing the same
WO1995004069A1 (fr) * 1993-07-30 1995-02-09 Affymax Technologies N.V. Biotinylation de proteines
WO1995025172A1 (fr) * 1994-03-17 1995-09-21 Universite Louis Pasteur Fragments d'anticorps recombinants synthetises et biotinyles dans e. coli, leur utilisation en immunodosages et en purification par immunoaffinite
WO2000040712A1 (fr) * 1999-01-07 2000-07-13 Medical Research Council Technique de tri optique

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
AN. ACAD. BRAS. CIENC. (1980), 52(3), 493-501 *
BIOMED. HEALTH RES. (1999), 27(ENZYMATIC MECHANISMS), 131-138 *
BIOORG. CHEM. (1986), 14(3), 249-61 *
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; AMSPACHER, DAVID R. ET AL: "Synthesis and characterization of a slow-binding inhibitor of biotin carboxylase", XP002164940, retrieved from STN Database accession no. 131:141217 *
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; AMSPACHER, DAVID R. ET AL: "Synthesis of a Reaction Intermediate Analogue of Biotin-Dependent Carboxylases via a Selective Derivatization of Biotin", XP002164939, retrieved from STN Database accession no. 131:157677 *
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; BERKESSEL, ALBRECHT ET AL: "On the structures of some adducts of biotin with electrophiles: does sulfur transannular interaction with the carbonyl group play a role in the chemistry or biochemistry of biotin?", XP002164941, retrieved from STN Database accession no. 106:15042 *
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; MONTEIRO, HUGO J.: "A stereospecific synthesis of dl-biotin using an intramolecular nitrone [2 + 3]-cycloaddition", XP002164942, retrieved from STN Database accession no. 94:208767 *
F.J. GERMINO ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 90, February 1993 (1993-02-01), NATIONAL ACADEMY OF SCIENCE. WASHINGTON., US, pages 933 - 937, XP002164938, ISSN: 0027-8424 *
ORG. LETT. (1999), 1(1), 99-102 *
PIRRUNG M C ET AL: "A General Method for the Spatially Defined Immobilization of Biomolecules on Glass Surfaces Using Caged Biotin", BIOCONJUGATE CHEMISTRY,US,AMERICAN CHEMICAL SOCIETY, WASHINGTON, vol. 7, no. 3, May 1996 (1996-05-01), pages 317 - 321, XP002095758, ISSN: 1043-1802 *
ZHEN X W ET AL: "Vectors for a @?double-tagging@? assay for protein-protein interactions: localization of the CDK2-binding domain of human p21", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 173, no. 2, 16 September 1996 (1996-09-16), pages 147 - 154, XP004043207, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
FR2816943B1 (fr) 2004-02-13
FR2816943A1 (fr) 2002-05-24
WO2002042311A2 (fr) 2002-05-30
AU2002222004A1 (en) 2002-06-03
US20040138446A1 (en) 2004-07-15
EP1347985A2 (fr) 2003-10-01

Similar Documents

Publication Publication Date Title
GB9803226D0 (en) Chemical compounds
SG82630A1 (en) 4,1-benzoxazepin derivatives and their use
AU9419601A (en) Nitrogenous five-membered ring compounds
MX235505B (fr)
WO2005087685A8 (fr) Agent réduisant le retrait de séchage
DK1008592T3 (da) Cykliske amidderivater, som inhiberer cathepsin K
EP1167367A4 (fr) Composes de thienopyrimidine et leurs sels, et procede de preparation desdits composes et sels
WO2002042311A3 (fr) Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs
NZ335252A (en) Substituted 3-thia-1,2,3,4-tetrahydrocarbazole, 6,7,8,9-tetrahydropyrido[1,2-a]indole, 4,5-dihydro-thiopyrano[3,4-b]indole, pyrano[3,4-b]indole or beta-carbolinyl derivatives
DE69809727D1 (de) Modifizierte isocyanate mit tensideigenschaften, diese enthaltende zusammensetzungen und daraus hergestellte beschichtungen
HK1071764A1 (en) Synthesis and use of alkylalkoxy acyloxysilanes and blends thereof
NZ510303A (en) Hydroxamate-containing cysteine and serine protease inhibitors suitable for treatment of neurodegeneration, Alzheimer's, inflammation, cataracts, bone resorption, CNS injury
HRP20031055B1 (en) Novel immunomodulating compounds
MXPA03010946A (es) Aditivos de hidracido de alquilo para lubricantes.
SI0668279T1 (en) 6-Azaindole thromboxane synthase inhibitors
DE60201903D1 (de) Eisenpulverzusammensetzung mit einem amid-schmiermittel und verfahren zu ihrer herstellung
MXPA02007973A (es) Compuestos de aminoacidos y su uso como inhibidores de nep, ace y ece.
TW349104B (en) A base resin for use in chemically amplified resist and a manufacturing method thereof the invention relates to a base resin for use in a chemically amplified resist and a manufacturing method thereof, the base resin containing two t-butyl groups
AU3648093A (en) Pyrrolidine derivatives useful as 5-HT3-antagonists
GB9715709D0 (en) Novel compounds
CA2118052A1 (fr) Derives du n-(aminosubstitue)pyrrole, leur procede de preparation et compositions herbicides
BR9911402A (pt) Uso de corantes amino azóicos, e, compostos
TW200502227A (en) Novel lactone compound
TW200510300A (en) Amino alcohol compounds
CA2362039A1 (fr) Nouveaux agents glissants et films de polypropylene prepares a partir de ces agents

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2001997495

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001997495

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 10432383

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2001997495

Country of ref document: EP